Academic literature on the topic 'Soil PLFA analysis'

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Journal articles on the topic "Soil PLFA analysis"

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Roslev, Peter, and Niels Iversen. "Radioactive Fingerprinting of Microorganisms That Oxidize Atmospheric Methane in Different Soils." Applied and Environmental Microbiology 65, no. 9 (1999): 4064–70. http://dx.doi.org/10.1128/aem.65.9.4064-4070.1999.

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ABSTRACT Microorganisms that oxidize atmospheric methane in soils were characterized by radioactive labelling with14CH4 followed by analysis of radiolabelled phospholipid ester-linked fatty acids (14C-PLFAs). The radioactive fingerprinting technique was used to compare active methanotrophs in soil samples from Greenland, Denmark, the United States, and Brazil. The 14C-PLFA fingerprints indicated that closely related methanotrophic bacteria were responsible for the oxidation of atmospheric methane in the soils. Significant amounts of labelled PLFAs produced by the unknown soil methanotrophs coe
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Johnsen, Anders R., Anne Winding, Ulrich Karlson, and Peter Roslev. "Linking of Microorganisms to Phenanthrene Metabolism in Soil by Analysis of 13C-Labeled Cell Lipids." Applied and Environmental Microbiology 68, no. 12 (2002): 6106–13. http://dx.doi.org/10.1128/aem.68.12.6106-6113.2002.

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ABSTRACT Phenanthrene-metabolizing soil microbial communities were characterized by examining mineralization of [14C]phenanthrene, by most-probable-number (MPN) counting, by 16S-23S spacer DNA analysis of the numerically dominant, culturable phenanthrene-degrading isolates, and by examining incorporation of [13C]phenanthrene-derived carbon into sterols and polar lipid fatty acids (PLFAs). An unpolluted agricultural soil, a roadside soil diffusely polluted with polycyclic aromatic hydrocarbons (PAHs), and two highly PAH-polluted soils from industrial sites were analyzed. Microbial phenanthrene
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Hanajík, Peter, Milan Zvarík, Hannu Fritze, Ivan Šimkovic, and Róbert Kanka. "Composition of microbial PLFAs and correlations with topsoil characteristics in the rare active travertine spring-fed fen." Ekológia (Bratislava) 35, no. 4 (2016): 295–308. http://dx.doi.org/10.1515/eko-2016-0024.

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Abstract We studied soil PLFAs composition and specific soil properties among transect of small-scale fen in Stankovany, Slovakia. The aim of this study was to determine potential differences in the microbial community structure of the fen transect and reveal correlations among PLFAs and specific soil characteristics. PCA analyses of 43 PLFAs showed a separation of the samples along the axis largely influenced by i14:0, 16:1ω5, br17:0, 10Me16:0, cy17:0, cy17:1, br18:0 and 10Me17:0. We measured a high correlation of sample scores and distance from fen edge (Kendall’s test τ = 0.857, P < 0.01
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Swallow, Mathew J. B., and Sylvie A. Quideau. "Evidence of rapid non-targeted effects of cycloheximide on soil bacteria using 13C-PLFA analysis." Canadian Journal of Soil Science 100, no. 4 (2020): 356–62. http://dx.doi.org/10.1139/cjss-2019-0152.

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Stable isotope probing of phospholipid fatty acids (PLFA-SIP) is useful when studying bacterial contributions to soil processes, and it is an effective way to separate fungal and bacterial activity by linking 13C enrichment to specific PLFAs. Distinguishing bacterial contributions to soil processes often employs selective inhibitors; however, studies demonstrating their efficacy when using PLFA-SIP are less common. Here, we determined the effect of the fungal inhibitor cycloheximide (4.8 mg g−1 dry soil) and the bacterial inhibitor bronopol (0.48 mg g−1 dry soil) on microbial communities white
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Hanson, Jessica R., Jennifer L. Macalady, David Harris, and Kate M. Scow. "Linking Toluene Degradation with Specific Microbial Populations in Soil." Applied and Environmental Microbiology 65, no. 12 (1999): 5403–8. http://dx.doi.org/10.1128/aem.65.12.5403-5408.1999.

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ABSTRACT Phospholipid fatty acid (PLFA) analysis of a soil microbial community was coupled with 13C isotope tracer analysis to measure the community’s response to addition of 35 μg of [13C]toluene ml of soil solution−1. After 119 h of incubation with toluene, 96% of the incorporated13C was detected in only 16 of the total 59 PLFAs (27%) extracted from the soil. Of the total 13C-enriched PLFAs, 85% were identical to the PLFAs contained in a toluene-metabolizing bacterium isolated from the same soil. In contrast, the majority of the soil PLFAs (91%) became labeled when the same soil was incubate
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Li, Xin, Yan Jiao, and Ming De Yang. "Diversity of Soil Microbial Communities under Different Soil Salinity Levels Analyzing by PLFA." Advanced Materials Research 955-959 (June 2014): 314–20. http://dx.doi.org/10.4028/www.scientific.net/amr.955-959.314.

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Under different soil salinity levels, diversity of soil microbial communities from Hetao irrigated land of Inner Mongolia was studied by phospholipid fatty acid (PLFA) analysis. The study found that PLFAs biomass in saline soil was significantly lower than those of strongly salinized soil and slight salinized soil. Microbes was bacteria-based from these soil. The bacterial PLFA loading in saline soil is significantly less than those of strongly salinized soil and slight salinized soil . Cluster analysis showed that changes had obviously taken place on soil microbial composition and quantity un
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Jiao, Hai Hua, Zhi Hui Bai, Ying Liu, Kai Wang, and Zhan Bin Huang. "Impact of Super Absorbent Polymer and Plants on Microbial Community and Petroleum Hydrocarbon Degradation in Contaminated Soil." Advanced Materials Research 807-809 (September 2013): 353–60. http://dx.doi.org/10.4028/www.scientific.net/amr.807-809.353.

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A greenhouse pot test, in which wheat, cabbage, spinach were cultivated separately in petroleum contaminated soil with and without super absorbent polymer (SAP), was conducted to evaluated the effect of plants and SAP on soil microbiological properties. phospholipid fatty acids (PLFAs) profiles were analyzed to reveal the microbial communities. As a measure of the functional activity of soil microbial community, the ratio of degraded to total petroleum hydrocarbon (TPH) in soil was estimated. The results indicated that SAP had an important effect on the soil microbial community and its degradi
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Jiao, Hai Hua, Kai Wang, Jian Gang Pan, De Cai Jin, Zhan Bin Huang, and Zhi Hui Bai. "Impact of Humics and Plants on Microbial Community and Petroleum Hydrocarbon Degradation in Contaminated Soil." Advanced Materials Research 726-731 (August 2013): 131–40. http://dx.doi.org/10.4028/www.scientific.net/amr.726-731.131.

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A greenhouse pot experiment, in which 3 different crops (wheat, cabbage, spinach) were cultivated in soil with and without humics (HS), was conducted to evaluate the effect of HS on soil microbiological properties. Phospholipid fatty acid (PLFA) profiles were analyzed to reveal the microbial community structure. As a measure of the functional activity of soil microbial community, the ratio of degraded to total petroleum hydrocarbon in soil was estimated. The results indicated that HS had an important effect on the soil microbial community and its functional activities. First, the principal com
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Guan, Huiling, Jiangwen Fan, Haiyan Zhang, and Warwick Harris. "Comparison of Drivers of Soil Microbial Communities Developed in Karst Ecosystems with Shallow and Deep Soil Depths." Agronomy 11, no. 1 (2021): 173. http://dx.doi.org/10.3390/agronomy11010173.

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Soil erosion is prevalent in karst areas, but few studies have compared the differences in the drivers for soil microbial communities among karst ecosystems with different soil depths, and most studies have focused on the local scale. To fill this research gap, we investigated the upper 20 cm soil layers of 10 shallow–soil depth (shallow–SDC, total soil depth less than 100 cm) and 11 deep–soil depth communities (deep–SDC, total soil depth more than 100 cm), covering a broad range of vegetation types, soils, and climates. The microbial community characteristics of both the shallow–SDC and deep–
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Bach, Lisbet Holm, Åsa Frostegård, and Mikael Ohlson. "Variation in soil microbial communities across a boreal spruce forest landscape." Canadian Journal of Forest Research 38, no. 6 (2008): 1504–16. http://dx.doi.org/10.1139/x07-232.

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We investigated soil microbial community structure by phospholipid fatty acid (PLFA) analysis in a mature boreal spruce forest landscape in southern Norway, with low diversity of vascular plants. We investigated the spatial variation in PLFAs and the importance of environmental variables in 10 plots (each 13 samples) in a study area of 1 km × 1 km. The scales investigated were 15 cm to 10 m within study plots and 100 m to 1 km between study plots. Soil microbial biomass varied 10-fold and we found a large variation in microbial community structure, even at distances of 15 cm. Samples aggregate
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Dissertations / Theses on the topic "Soil PLFA analysis"

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FERRE', CHIARA. "Monitoring of greenhouse gas emissions from agricultural and forest soils." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2009. http://hdl.handle.net/10281/7483.

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Global climate change is becoming a central issue in contemporary science as well as politics. There is a long-lasting debate about the cause of the climate change: anthropogenic activity versus the natural cycle. However, a scientific consensus is coming a conclusion that the contemporary climate change is mainly caused by anthropogenic emissions of the greenhouse gases (GHG), including carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4). The main objective of the thesis is the monitoring of such GHG emissions from two ecosystem types: a forest and a rice paddy ecosystem. The fores
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Hendricks, Luanne R. "Soil Bioavailability of Aminomethylphosphonic Acid: A Metabolite of Glyphosate." The Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1592583268434087.

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LISBOA, Francy Junio Gon?alves. "Uso da abordagem estat?stica procrusteana em Ecologia de Solo: caso de estudo envolvendo sistema de integra??o lavoura-pecu?ria-floresta no Cerrado." Universidade Federal Rural do Rio de Janeiro, 2015. https://tede.ufrrj.br/jspui/handle/jspui/1570.

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Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-05-02T21:19:46Z No. of bitstreams: 1 2015 - Francy Junio Gon?alves Lisboa.pdf: 2939884 bytes, checksum: c4aa4152d0d3ca90c85ecb78ff7e5da6 (MD5)<br>Made available in DSpace on 2017-05-02T21:19:46Z (GMT). No. of bitstreams: 1 2015 - Francy Junio Gon?alves Lisboa.pdf: 2939884 bytes, checksum: c4aa4152d0d3ca90c85ecb78ff7e5da6 (MD5) Previous issue date: 2015-02-25<br>CAPES<br>This thesis is part of a multiple scientific effort seeking to support the replacement of degraded brazilian pastures by systems which integrate different land use
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Románeková, Ivana. "Stanovení mikroplastů PLA v půdě pyrolýzními metodami." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2020. http://www.nusl.cz/ntk/nusl-413552.

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Nowadays, plastic waste poses one of the greatest risks to the environment. Plastics affect the environment at all stages of their life cycle. Bioplastics have become widely used as a substitute for conventional plastics, without detailed examination of their behavior in real environmental conditions. As a result, it is assumed that they can accumulate in the environment and the question arose as to how to identify them. The main goal of this thesis is to develop a method based on sample pyrolysis that is suitable for the identification and determination of the amount of PLA microplastics in s
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Baretta, Carolina Riviera Duarte Maluche. "Diversidade microbiana em solos sob florestas de Araucaria angustifolia." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/11/11140/tde-06032008-163038/.

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A Floresta Ombrófila Mista também chamada de Floresta de Araucária representa um dos mais ricos remanescentes de florestas pluviais subtropicais brasileiras, tendo como principal representante a Araucaria angustifolia, espécie considerada ameaçada de extinção. A diversidade microbiana possui um importante papel no funcionamento e manutenção do equilíbrio dos ecossistemas florestais, mas é desconhecida em solos com araucária. O presente estudo teve como objetivo avaliar a diversidade, funcionalidade e estrutura das comunidades microbianas em florestas de Araucaria angustifolia natural, introduz
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KOTAS, Petr. "Various aspects of soil microbial ecology as revealed by phospholipid fatty acid (PLFA) analysis." Doctoral thesis, 2018. http://www.nusl.cz/ntk/nusl-373873.

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The PLFA profiling method was adopted and used to determine changes in microbial community structure and abundance along natural and human-induced environmental gradients. The presented studies were based on field sampling campaigns combined with targeted laboratory experiments. According to the aims of particular studies, microbial PLFA fingerprinting was combined with the auxiliary below- and aboveground ecosystem characteristics to identify the drivers of microbial responses to environmental changes or with 13C-labelling and metagenomics to obtain more complex information about running proc
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Conference papers on the topic "Soil PLFA analysis"

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Géczy, Attila, Lívia Gyenizse, Csaba Farkas, Dániel Rigler, Balázs Sáfár, and András Csiszár. "Biodegradable PCBs with PLA/Flax Substrate: FTIR and SEM Analysis of Soil Degradation." In 2024 IEEE 10th Electronics System-Integration Technology Conference (ESTC). IEEE, 2024. http://dx.doi.org/10.1109/estc60143.2024.10712063.

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Mariam Paul, Nivya, and Variampally Sankar Harikumar. "Effects of biochar on soil microbial community composition using PLFA profiling- A review." In 7th GoGreen Summit 2021. Technoarete, 2021. http://dx.doi.org/10.36647/978-93-92106-02-6.5.

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Biochar is a charcoal like substance produced from organic biomass after pyrolysis. Biochar act as a good soil conditioner by increasing microbial activities, soil nutrition and soil structure. Soil microorganisms are involved in litter decomposition and soil nutrient mineralization which is important in the sustainable development of plants and trees. The functioning of an ecosystem is controlled by biogeochemical cycles driven by microorganisms. The cell membrane of all microorganisms is composed of phospholipids that are easily metabolized after the cell death. Hence, phospholipid fatty aci
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Jie Liu, Xiawei Peng, and Zhihui Bai. "Effect of pyrene contamination on soil microbial biomass and community structure using PLFA analysis." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5965917.

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Liu, Ruihua, Yan Wang, Yingying Liu, Huifen Liu, and Jianning Zhao. "The effect of transgenic cotton planting on soil microbial community structure based on PLFA analysis." In 2018 7th International Conference on Energy, Environment and Sustainable Development (ICEESD 2018). Atlantis Press, 2018. http://dx.doi.org/10.2991/iceesd-18.2018.50.

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