Relevant bibliographies by topics / SMIM10

Academic literature on the topic 'SMIM10'

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Published: 14 March 2023

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Journal articles on the topic "SMIM10"

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Kulinska, Karolina Iwona, Mirosław Andrusiewicz, Anna Dera-Szymanowska, Maria Billert, Marek Skrzypski, Krzysztof Szymanowski, Ewa Nowak-Markwitz, Małgorzata Kotwicka, and Maria Wołuń-Cholewa. "Phoenixin as a New Target in the Development of Strategies for Endometriosis Diagnosis and Treatment." Biomedicines 9, no. 10 (October 9, 2021): 1427. http://dx.doi.org/10.3390/biomedicines9101427.

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Small integral membrane protein 20/phoenixin (SMIM20/PNX) and its receptor GPR173 (G Protein-Coupled Receptor 173) play a role in the regulation of the hypothalamic–pituitary–gonadal axis (HPG). The aim of the study was to determine PNX, FSH, LH, and 17β-estradiol association in women with endometriosis, and the expression of SMIM20/PNX signaling via GPR173. Serum PNX, FSH, LH, and 17β-estradiol concentrations were measured by enzyme and electrochemiluminescence immunoassay. SMIM20/PNX and GPR173 expression in the eutopic and ectopic endometrium was assessed by qPCR and immunohistochemistry. Reduced PNX level, increased LH/FSH ratio and elevated 17β-estradiol concentration were found in patients with endometriosis. No differences in SMIM20 expression were observed between the studied endometria. GPR173 expression was lower in ectopic than in eutopic endometria. SMIM20 expression was mainly restricted to stroma. GPR173 was detected in some eutopic and ectopic stromal cells and in eutopic glandular epithelial cells. Discriminant analysis indicates the diagnostic relevance of PNX and LH/FSH ratio in patients with endometriosis. In women with endometriosis, reduced PNX levels and GPR173 expression may be responsible for HPG axis dysregulation. These new insights may contribute to a better understanding of the pathophysiology of endometriosis and provide the basis for a new strategy for diagnosis and treatment of endometriosis.
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Nett, Jeniel E., Hiram Sanchez, Michael T. Cain, Kelly M. Ross, and David R. Andes. "Interface of Candida albicans Biofilm Matrix-Associated Drug Resistance and Cell Wall Integrity Regulation." Eukaryotic Cell 10, no. 12 (June 10, 2011): 1660–69. http://dx.doi.org/10.1128/ec.05126-11.

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ABSTRACTCandida albicansfrequently infects medical devices by growing as a biofilm, i.e., a community of adherent organisms entrenched in an extracellular matrix. During biofilm growth,Candidaspp. acquire the ability to resist high concentrations of antifungal drugs. One recently recognized biofilm resistance mechanism involves drug sequestration by matrix β-1,3 glucan. Using a candidate gene approach, we investigated potentialC. albicansβ-1,3-glucan regulators, based on their homology toSaccharomyces cerevisiae, includingSMI1and protein kinase C (PKC) pathway components. We identified a role for theSMI1in biofilm matrix glucan production and development of the associated drug resistance phenotype. This pathway appears to act through transcription factor Rlmp and glucan synthase Fks1p. The phenotypes of these mutant biofilms mimicked those of thesmi1Δ/smi1Δ biofilm, and overexpression ofFKS1in thesmi1Δ/smi1Δ mutant restored the biofilm resistant phenotype. However, control of this pathway is distinct from that of the upstream PKC pathway because thepkc1Δ/pkc1Δ,bck1Δ/bck1Δ,mkk2Δ/mkk2Δ, andmkc1Δ/mkc1Δ biofilms retained the resistant phenotype of the parent strain. In addition, resistance to cell-perturbing agents and gene expression data do not support a significant role for the cell wall integrity pathway during the biofilm formation. Here we show that Smi1p functions in conjunction with Rlm1p and Fks1p to produce drug-sequestering biofilm β-glucan. Our work provides new insight into how theC. albicansbiofilm matrix production and drug resistance pathways intersect with the planktonic cell wall integrity pathway. This novel connection helps explain how pathogens in a multicellular biofilm community are protected from anti-infective therapy.
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Brilhante, Raimunda Sâmia Nogueira, Xhaulla Maria Quariguasi Cunha Fonseca, Vandbergue Santos Pereira, Géssica dos Santos Araújo, Jonathas Sales de Oliveira, Lana Glerieide Silva Garcia, Anderson Messias Rodrigues, et al. "In vitro inhibitory effect of statins on planktonic cells and biofilms of the Sporothrix schenckii species complex." Journal of Medical Microbiology 69, no. 6 (June 1, 2020): 838–43. http://dx.doi.org/10.1099/jmm.0.001195.

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Introduction. Sporotrichosis, caused by species of the Sporothrix schenckii complex, is the most prevalent subcutaneous mycosis in many areas of Latin America. Statins are a class of drugs widely used for lowering high sterol levels through their action on 3-hydroxy-3-methylglutaryl-CoA reductase, a key enzyme in the synthesis of sterol. Aim. In this study, the antifungal activity of statins (simvastatin, atorvastatin, pravastatin) against planktonic cells and biofilms of S. schenckii complex species was evaluated, as well as the interaction of pravastatin with classical antifungals (amphotericin B, itraconazole, terbinafine). Methodology. Eighteen strains of Sporothrix species were used. The antifungal susceptibility assay was performed using the broth microdilution method. Mature biofilms were exposed to statins and metabolic activity was measured by the XTT reduction assay. Results. MICs of statins ranged from 8 to 512 μg ml−1 and from 8 to 256 μg ml−1 for filamentous and yeast forms, respectively. Regarding mature biofilms, MICs of 50 % inhibition (SMIC50) were 128 μg ml−1 for simvastatin and atorvastatin and >2048 μg ml−1 for pravastatin. MICs of 90 % inhibition (SMIC90) were 512 μg ml−1 for simvastatin and >2048 μg ml−1 for atorvastatin and pravastatin. Conclusion. These results highlight the antifungal and antibiofilm potential of statins against S. schenckii complex species.
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Yu, Ying, Zhejiong Wang, Linchao Zhu, Yushiang Lin, Haochun Chang, and Huaxi Xu. "The Polymorphism of SMIM1 Gene in Chinese Dividuals." Indian Journal of Hematology and Blood Transfusion 35, no. 1 (June 21, 2018): 137–43. http://dx.doi.org/10.1007/s12288-018-0963-8.

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Ballif, Bryan A., Virginie Helias, Thierry Peyrard, Cécile Menanteau, Carole Saison, Nicole Lucien, Sébastien Bourgouin, Maude Le Gall, Jean‐Pierre Cartron, and Lionel Arnaud. "Disruption of SMIM1 causes the Vel− blood type." EMBO Molecular Medicine 5, no. 5 (April 15, 2013): 751–61. http://dx.doi.org/10.1002/emmm.201302466.

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Rajeswari, Jithine Jayakumar, Ayelén Melisa Blanco, and Suraj Unniappan. "Phoenixin-20 suppresses food intake, modulates glucoregulatory enzymes, and enhances glycolysis in zebrafish." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 318, no. 5 (May 1, 2020): R917—R928. http://dx.doi.org/10.1152/ajpregu.00019.2020.

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Phoenixin is a 20-amino acid peptide (PNX-20) cleaved from the small integral membrane protein 20 (SMIM20), with multiple biological roles in mammals. However, its role in nonmammalian vertebrates is poorly understood. This research aimed to determine whether PNX-20 influences feeding and metabolism in zebrafish. The mRNAs encoding SMIM20 and its putative receptor, super conserved receptor expressed in brain 3 (SREB3), are present in both central and peripheral tissues of zebrafish. Immunohistochemical analysis confirmed the presence of PNX-like immunoreactivity in the gut and in zebrafish liver (ZFL) cell line. We also found that short-term fasting (7 days) significantly decreased smim20 mRNA expression in the brain, gut, liver, gonads, and muscle, which suggests a role for PNX-20 in food intake regulation. Indeed, single intraperitoneal injection of 1,000 ng/g body wt PNX-20 reduced feeding in both male and female zebrafish, likely in part by enhancing hypothalamic cart and reducing hypothalamic/gut preproghrelin mRNAs. Furthermore, the present results demonstrated that PNX-20 modulates the expression of genes involved in glucose transport and metabolism in ZFL cells. In general terms, such PNX-induced modulation of gene expression was characterized by the upregulation of glycolytic genes and the downregulation of gluconeogenic genes. A kinetic study of the ATP production rate from both glycolytic and mitochondrial pathways demonstrated that PNX-20-treated ZFL cells exhibited significantly higher ATP production rate associated with glycolysis than control cells. This confirms a positive role for PNX-20 on glycolysis. Together, these results indicate that PNX-20 is an anorexigen with important metabolic roles in zebrafish.
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Davis, D. R., J. P. Brion, A. M. Couck, J. M. Gallo, D. P. Hanger, K. Ladhani, C. Lewis, et al. "The phosphorylation state of the microtubule-associated protein tau as affected by glutamate, colchicine and β-amyloid in primary rat cortical neuronal cultures." Biochemical Journal 309, no. 3 (August 1, 1995): 941–49. http://dx.doi.org/10.1042/bj3090941.

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The effects of the excitatory amino acid glutamate, the microtubule destabilizing agent colchicine, and beta 25-35-amyloid peptide on the phosphorylation state of tau were studied in rat cortical neurons in primary culture. Using immunocytochemistry and Western-blot analysis, we demonstrated that a proportion of tau in these cultures is normally highly phosphorylated, but most of this tau fraction is dephosphorylated after treatment of the cultures with glutamate or colchicine, but not with beta-amyloid; the glutamate- and colchicine-induced changes in tau phosphorylation commenced before cell death, as assessed by release of lactate dehydrogenase. Dephosphorylation of tau was readily revealed by using the monoclonal antibodies Tau.1 and AT8, which have phosphate-sensitive epitopes that both centre around serine-199 and -202 (numbering of the largest tau isoform). On Western blots and by immunocytochemistry, AT8 labelling strongly decreased after glutamate and colchicine treatments, whereas Tau.1 staining was more intense. Neurofilament monoclonal antibodies, including RT97, 8D8, SMI31 and SMI310, all additionally known to recognize tau in a phosphorylation-dependent manner, also demonstrated that glutamate and colchicine treatments of the cultures induced a dephosphorylation of tau. We also showed immunocytochemically that there is an increase in tau immunoreactivity in neuronal perikarya in response to glutamate and colchicine treatment, and this occurs concomitantly with the dephosphorylation of tau. Treatment of the primary rat cortical neuronal cultures with beta 25-35-amyloid peptide, under conditions which induce neuronal degeneration, did not induce a change in tau phosphorylation, and failed to act synergistically with glutamate to produce an increase in dephosphorylation of tau over that produced by glutamate treatment alone. These findings demonstrate that glutamate and colchicine induce tau dephosphorylation, as opposed to increased tau phosphorylation, which would be more indicative of Alzheimer-type neurodegeneration.
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Unfried, Juan Pablo, and Puri Fortes. "SMIM30, a tiny protein with a big role in liver cancer." Journal of Hepatology 73, no. 5 (November 2020): 1010–12. http://dx.doi.org/10.1016/j.jhep.2020.07.015.

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Barrese, Vincenzo, Jennifer B. Stott, Samuel N. Baldwin, Gema Mondejar-Parreño, and Iain A. Greenwood. "SMIT (Sodium-Myo-Inositol Transporter) 1 Regulates Arterial Contractility Through the Modulation of Vascular Kv7 Channels." Arteriosclerosis, Thrombosis, and Vascular Biology 40, no. 10 (October 2020): 2468–80. http://dx.doi.org/10.1161/atvbaha.120.315096.

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Objective: The SMIT1 (sodium:myo-inositol transporter 1) regulates myo-inositol movement into cells and responses to hypertonic stimuli. Alteration of myo-inositol levels has been associated with several diseases, including hypertension, but there is no evidence of a functional role of SMIT1 in the vasculature. Recent evidence showed that in the nervous system SMIT1 interacted and modulated the function of members of the Kv7 family of voltage-gated potassium channels, which are also expressed in the vasculature where they regulate arterial contractility. Therefore, in this study, we evaluated whether SMIT1 was functionally relevant in arterial smooth muscle. Approach and Results: Immunofluorescence and polymerase chain reaction experiments revealed that SMIT1 was expressed in rat renal and mesenteric vascular smooth muscle cells. Isometric tension recordings showed that incubation of renal arteries with raffinose and myo-inositol (which increases SMIT1 expression) reduced the contractile responses to methoxamine, an effect that was abolished by preincubation with the pan-Kv7 blocker linopirdine and by molecular knockdown of Kv7.4 and Kv7.5. Knockdown of SMIT1 increased the contraction of renal arteries induced by methoxamine, impaired the response to the Kv7.2–Kv7.5 activator ML213 but did not interfere with the relaxant responses induced by openers of other potassium channels. Proximity ligation assay showed that SMIT1 interacted with heteromeric channels formed by Kv7.4 and Kv7.5 proteins in both renal and mesenteric vascular smooth muscle cells. Patch-clamp experiments showed that incubation with raffinose plus myo-inositol increased Kv7 currents in vascular smooth muscle cells. Conclusions: SMIT1 protein is expressed in vascular smooth muscle cells where it modulates arterial contractility through an association with Kv7.4/Kv7.5 heteromers.
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Aniweh, Yaw, Prince B. Nyarko, Evelyn Quansah, Laty Gaye Thiam, and Gordon A. Awandare. "SMIM1 at a glance; discovery, genetic basis, recent progress and perspectives." Parasite Epidemiology and Control 5 (May 2019): e00101. http://dx.doi.org/10.1016/j.parepi.2019.e00101.

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Dissertations / Theses on the topic "SMIM10"

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LUBRANO, SIMONE. "Yeast S. cerevisiae as a tool to study BRAFV600E kinase isoforms." Doctoral thesis, Università di Siena, 2018. http://hdl.handle.net/11365/1040150.

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BRAFV600E causes an altered regulation of the MAPK pathway (mitogen-activated protein kinase or RAS / RAF / MEK / ERK pathway), involved in cell division and differentiation. This mutation consists of the substitution of a valine with glutamic acid at position 600 (V600E), resulting in a change in conformation, responsible for a constitutive activation of the protein, even in the presence of a low level of RAS, its activator. In this work we have demonstrated, for the first time, that hBRAFV600E kinase activity is preserved and can be studied in yeast. Indeed, hBRAFV600E complements the activity of MAPKKK kinases belonging to the osmotic stress (HOG) pathway and is toxic in yeast strains deleted for phosphatases of the same pathway. Moreover, we have demonstrated that a yeast genetic context allows the one-by-one analysis of the 3 BRAF protein isoforms that always coexist in human cells (BRAF-Ref, BRAF-X1 and BRAF-X2). In addition, we provide experimental evidence that yeast can be used to perform high throughput screenings and identify new BRAFV600E functional interactors. In fact, two screenings have been performed in this model system, one using a cDNA Library and another one taking advantage of the Yeast Deletion Pool collection. The screening performed with the cDNA library, deriving from HeLa cells, was performed in a yeast strain deleted for two phosphates PTC1 and PTP3. ~105 transformed cells have been screened. We obtained 16 complete CDS, 13 out of 16 have been validated using the spot assay. Among them, we found the Small Integral Membrane Protein 10 (SMIM10), a protein of unknown function that has been further characterized because of its different expression levels in human melanoma cell lines with mutated BRAF as compared to those with wild type (wt) BRAF. Interestingly, SMIM10 overexpression causes a dramatic decrease in the levels of BRAFV600E both in yeast and in human cells. These results suggest that SMIM10 is a negative functional interactors (FI) of BRAFV600E with a possible oncosuppressive role. The second screening was performed using the Yeast Deletion Pool, a pool of yeast S. cerevisiae clones, that has a deletion in non-essential genes (4,741 clones). Each clone is identifiable by means of two specific DNA sequences called "barcodes". 4 Through the use of the yeast deletion pool, it is possible to identify functional interactors of proteins related to diseases which do not have homologous counterparts in yeast, such as BRAF. As a result of this screening, we have identified 9 genes affecting the fitness of cells expressing BRAFV600E-X1/Ref, compared to cells transformed with the empty vector. Four out of nine affected the fitness with both isoforms. Interestingly, among deleted genes altering the fitness of BRAFV600E expressing cells when deleted, there are RAS, a SWI/SNF remodeling factor and Arf2 (GTPase). The addition of salt to the growth of the YDP highlighted differences between the two isoforms. In fact, while the comparison pYES2-BRAFV600E-Ref versus pYES2-BRAFV600E-Ref + NaCl showed differences in 4 clones, the comparison of pYES2-BRAFV600E-X1 versus pYES2-BRAFV600E-X1+ NaCl showed differences in 21 clones. Among them, an ABC transporter, a Rho GTPase, and a subunit of TORC2 membrane-associated complex have been found. Finally, this work has yielded a list of modulators of BRAFV600E to be further characterized experimentally and eventually translated into innovative therapeutic strategies that could be used in addition to the existing BRAF inhibitors.
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Abdellaoui, El. "Pratiques agricoles et dynamique socio-techniques: cas des éleveurs agriculteurs de la commune rurale de Ben Smim Moyen Atlas Maroc." Doctoral thesis, Universite Libre de Bruxelles, 2005. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210904.

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L’agriculture est un secteur d’activité privilégié pour notre objet qui est l’étude de la dynamique sociotechnique et du travail. Nous montrons dans cette étude comment des éleveurs transformés de plus en plus en agro-pasteurs, à la suite de la sécheresse et la surcharge des hommes et du cheptel sur les ressources naturelles des parcours collectifs, sont amenés à changer progressivement leurs systèmes de production et partant leurs rapports sociaux.

Au-delà d’une vision figée et homogénéisante de la paysannerie véhiculée par certains modèles sociologiques et par la vulgarisation agricole au Maroc, nous mettons l’accent sur l’hétérogénéité de la paysannerie et les aspects dynamiques de l’activité agricole et de ses acteurs.

Bien que les éleveurs/agriculteurs évoluent dans un environnement physique et économique souvent défavorable à leurs activités, ils manifestent de différentes stratégies pour améliorer leurs conditions de vie ou renforcer leurs acquis.

A partir d’une étude sur le terrain rurale de la Commune de Ben Smim, au Moyen Atlas berbère marocain et ayant mobilisé différents instruments de recueil d’informations, nous avons relevé que l’activité agricole n’est pas simplement une activité de production mais aussi de repositionnement des acteurs dans le système social. L’ethnique, le social et le politique se mêlent dans l’orientation des rapports de production. C’est pourquoi il est difficile d’isoler une pratique agricole des autres pratiques qui lui sont intimement liées et qui peuvent concerner d’autres domaines de vie des agriculteurs.

Avec la crise du nomadisme, les éleveurs/agriculteurs se fixent dans les douars ou les villages et élargissent ainsi leurs réseaux sociaux et professionnels. Ils deviennent ainsi de plus en plus perméables aux innovations techniques et organisationnelles et améliorent la performance de leurs troupeaux, introduisent de nouvelles cultures de marché et diversifient leurs stratégies de vente. Les minorités du point de vue ethnique et économique, d’intégration dans le système social local, les notables sont à même d’apporter de nouvelles variantes à leurs systèmes de production.

En fin de compte, chacun, en fonction de sa situation et de son projet, participe à la dynamique socio-technique locale.


Doctorat en sciences sociales, Orientation sciences du travail
info:eu-repo/semantics/nonPublished

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Díaz, Franulic Ignacio. "Efecto de la Normalización de la Expresión del Transportador Na+/Mioinositol SMIT1 Sobre la Función Colinérgica de una Línea Celular Neuronal Derivada de Corteza Cerebral de la Trisomía 16 Murina (Modelo del Síndrome de Down Humano)." Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/105662.

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El Síndrome de Down (SD) se determina por la trisomía del autosoma 21 humano, y sus alteraciones se asocian a sobreexpresión o bien desregulación de los productos génicos derivados de este cromosoma. La trisomía 16 (Ts16) murina es un modelo animal de este síndrome, debido a la gran homología entre los cromosomas humano y murino correspondiente. Desafortunadamente, la condición en el modelo murino no es viable. Frente a esto se han generado líneas celulares inmortalizadas derivadas de corteza de ratón normal y trisómico 16, denominadas CNh y CTb respectivamente. Estas líneas celulares sobreexpresan genes relacionados con el SD y muestran función colinérgica alterada (menor incorporación de colina de alta afinidad (IC), expresión de la acetilcolintransferasa (ChAT) y liberación fraccional de acetilcolina (LF) frente a una estimulación), similares a las previamente encontradas en cultivos primarios de corteza Ts16. También se han encontrado elevados niveles de Mioinositol en pacientes con SD, lo cual estar relacionado con la sobreexpresión del Transportador Na+ /Mioinositol SMIT1, cuyo gen se encuentra ubicado en cromosoma 16 murino. Con el objeto de dilucidar la participación de SMIT1 en la disfunción colinérgica descrita anteriormente, decidimos reducir la expresión de SMIT1 transfectando las células con secuencias de antisentido específicos para mRNA en nuestras líneas celulares derivadas de corteza cerebral de Ts16 (CTb) comparadas con la línea derivada de un animal normal (CNh). Posterior a la transfección, estudios de Western Blot (WB) mostraron una reducción en la expresión de SMIT1 en 10%, 40%, y 42% a 24, 48 y 10 72 horas respectivamente, comparadas con niveles de SMIT1 en cultivos de la líneas celulares trisómicas CTb sin transfectar (n=6 p<0,01). Una vez normalizada la expresión de SMIT1, estudiamos la IC en CTb y CNh, encontrando que la línea CTb exhibe una reducción en la IC de un 80%, 85% y a 2 y 5 minutos de incubación con 1µCi de [3 H]- colina respectivamente, comparada con CNh. A 72 horas post-transfección los niveles en la IC fueron esencialmente similares en ambos tipos celulares. Finalmente, la LF, la cual está reducida en CTb comparada con CNh después de una despolarización generada por glutamato, nicotina , muscarina y KCl, , muestra un progresivo incremento después del Knockdown del SMIT al ser estimulada con nicotina y muscarina, mostrando valores similares a CNh después de 48 horas post-transfección. Sin embargo, al inducir despolarización con KCl y estimular con Glutamato extracelular, las líneas CTB tranfectadas no exhibieron diferencias con respecto a la línea celular trisómica CTb sin transfectar. Los resultados sugieren que la normalización en la expresión de SMIT1 en la línea CTb mediante la transfección con oligonucleótidos antisentido revierten: 1) la reducción de la incorporación de 3H colina, y 2) el déficit en la liberación de acetilcolina frente a agonistas Colinérgicos (Nicotina y Muscarina). Los resultados pueden constituir un punto de partida para el diseño de una terapia farmacológica que apunte a recuperar las funciones cognitivas, en particular las relacionados con sistemas colinérgicos, en pacientes con SD.
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Aouameur, Rym. "Caractérisation du co-transporteur Na+/myo-inositol SMIT2 dans les membranes en bordure en brosse de rein de lapin et d’intestin de rat." Thèse, 2009. http://hdl.handle.net/1866/2846.

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Le myo-inositol (MI) est un soluté organique impliqué dans diverses fonctions physiologiques de la cellule dont la signalisation cellulaire. Il est également un osmolyte compatible reconnu. Trois co-transporteurs de type actif secondaire responsables de son absorption ont été identifiés. Deux d’entre eux sont couplés au transport du sodium (SMIT1 et SMIT2) et le troisième est couplé au transport de protons (HMIT). L’objectif de cette étude a été la caractérisation du transport du MI par SMIT2 dans des membranes en bordure en brosse (BBMv) issues du rein de lapin et de l’intestin de rat ainsi qu’après expression dans les ovocytes de Xenopus laevis. La quantification de l’ARNm de SMIT1 et de SMIT2 dans le rein nous a appris que SMIT1 est majoritairement présent dans la médullaire alors que SMIT2 est principalement localisé dans le cortex. Ces résultats ont été confirmés par immunobuvardage en utilisant un anticorps dirigé contre SMIT2. Grâce à l’inhibition sélective de SMIT1 par le L-Fucose et de SMIT2 par le D-chiro-inositol (DCI), nous avons démontré que SMIT2 semble le seul responsable du transport luminal de MI dans le tubule contourné proximal avec un Km de 57 ± 14 µM. Pour ce qui est de l’intestin, des études de transport de MI radioactif ont démontré une absence de transport de MI chez le lapin alors que l’intestin de rat présente un transport de MI très actif. Une quantification par qRT-PCR nous a permis de constater que l’intestin de lapin ne semble pas posséder les transporteurs de MI nécessaires. Comme pour le rein, SMIT2 semble le seul transporteur de MI présent au niveau du pôle apical des entérocytes intestinaux chez le rat. Il est chargé du prélèvement du MI de l'alimentation avec un Km de 150 ± 40 µM. Les analyses fonctionnelles exécutées sur SMIT2 de rat en électrophysiologie après expression dans les ovocytes de Xenopus laevis donnent sensiblement les mêmes résultats que pour les BBMv de rein de lapin et d’intestin de rat. Dans les ovocytes, SMIT2 présente une grande affinité pour le MI (270 ± 19 µM) et le DCI (310 ± 60 µM) et aucune affinité pour le L-fucose. Il est ii également très sensible à la phlorizine (16 ± 7 µM). Une seule exception persiste : la constante d’affinité pour le glucose dans les BBMv d’intestin de rat est 40 fois plus petite que celle observée sur les ovocytes de Xenopus laevis. Nous avons également testé la capacité de certains transporteurs de sucre présents à la surface des membranes apicales des entérocytes à prélever le MI. Vu que l'inhibition de ces transporteurs (SGLT1 et GLUT5) ne changeait rien au taux de MI radioactif transporté, nous en avons conclu qu'ils ne sont pas impliqués dans son transport. Finalement, l’efflux de MI à partir du pôle basolatéral des entérocytes n’est pas effectué par GLUT2 puisque ce dernier lorsqu'il est exprimé dans des ovocytes, est incapable de transporter le MI.
Myo-inositol (MI) is an organic solute involved in various aspects of cell physiology, including cell signaling. It is also known as a compatible osmolyte. Three secondary active MI cotransporters have been identified; two are Na+- coupled (SMIT1 and SMIT2) and one is H+-coupled (HMIT). The main aim of this study was to characterize MI uptake throught SMIT2 as expressed in epithelial cells and in Xenopus laevis oocytes. In order to achieve the characterization of this transport system, we used purified brush border membrane vesicles (BBMv) isolated from rabbit kidney and rat intestine. We first performed a quantification of mRNA levels in rabbit kidney using real time PCR for both SMIT1 and SMIT2. We found that SMIT1 is mainly expressed in the renal medulla while SMIT2 is mainly localized in the renal cortex. This result was confirmed on Western blots using an antibody raised against SMIT2. Through inhibition studies using selective substrates for SMIT1 (inhibited by L-fucose) and SMIT2 (inhibited by D-chiroinositol), we showed that SMIT2 seems to be responsible for all the apical transport of MI into the proximal convoluted tubule with a Km of 57 ± 14 µM. By transport studies we established that rabbit intestine seems to lack apical transport of MI while rat intestine has a very active uptake of this molecule. qRT-PCR quantification confirmed the absence of MI transporters in rabbit intestine. As for kidney, SMIT2 seems to be the only transporter responsible for apical MI uptake in enterocytes with a Km of 150 ± 40 µM. Functional analysis of rat SMIT2 activity, via electrophysiological studies in Xenopus oocytes, demonstrated similarities to the activities of SMIT2 from rat intestine and rabbit kidney. SMIT2 displays high affinities for MI (270 ± 19 µM), DCI (310 ± 60 µM) and no affinity for L-fucose. SMIT2 is very sensitive to phlorizin (Pz; 16 ± 7 µM). Although these functional characteristics essentially confirmed those found in rat intestine, a iv discrepancy exists between the two systems studied. Indeed, the affinity constant for glucose was approximately 40-fold lower in vesicles than in oocytes. We also tested the ability of SGLT1 and GLUT5, other sugar transport systems present in enterocytes apical membranes, to perform MI uptake. Because the inhibition of these transporters did not alter radiolabeled MI uptake, we concluded that they had no significant contribution to MI transport in rat intestine. Finally, the basolateral efflux of MI was not mediated by GLUT2 because when expressed in oocytes, this transporter was not able to transport MI.
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Books on the topic "SMIM10"

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Bystrov, Peter I., and Vadim N. Sadovsky. Philosophical Logic and Logical Philosophy: Essays in Honour of Vladimir A. Smimov. Springer, 2010.

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Book chapters on the topic "SMIM10"

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Bosch, Karl. "Kolmogoroff-Smimov-Test — Wahrscheinlichkeitspapier." In Aufgaben und Lösungen zur angewandten Statistik, 88–91. Wiesbaden: Vieweg+Teubner Verlag, 1986. http://dx.doi.org/10.1007/978-3-322-85064-5_19.

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Bosch, Karl. "Kolmogoroff-Smimov-Test — Wahrscheinlichkeitspapier." In Aufgaben und Lösungen zur angewandten Statistik, 30–31. Wiesbaden: Vieweg+Teubner Verlag, 1986. http://dx.doi.org/10.1007/978-3-322-85064-5_7.

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Dawar, Siddharth, Vikram Goyal, and Debajyoti Bera. "SMIM Framework to Generalize High-Utility Itemset Mining." In Advanced Data Mining and Applications, 3–15. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-95408-6_1.

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Wang, Jiaming, Tao Lu, and Yanduo Zhang. "SMIM: Superpixel Mutual Information Measurement for Image Quality Assessment." In Algorithms and Architectures for Parallel Processing, 432–44. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-030-05054-2_34.

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Rubin, Kurt A., Yongliang Yang, Oskar Amster, David A. Scrymgeour, and Shashank Misra. "Scanning Microwave Impedance Microscopy (sMIM) in Electronic and Quantum Materials." In Electrical Atomic Force Microscopy for Nanoelectronics, 385–408. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-15612-1_12.

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Raj, Lakshmi, and Mallika Sankar M. "Exploring the Role of Social Media Influencer Marketing in the Tourism Sector." In Handbook of Research on Sustainable Tourism and Hotel Operations in Global Hypercompetition, 100–117. IGI Global, 2022. http://dx.doi.org/10.4018/978-1-6684-4645-4.ch005.

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The growing popularity of social media influencers (SMI) increasingly encourages destinations to use social media influencer marketing (SMIM) for their promotional campaigns. SMIs demonstrate the power of an individual based on certain factors employed to influence a broad segment of audience, and when it is used for marketing purposes, it is known as SMIM. SMIM is a part of the social media marketing that exercises all the commercial marketing techniques via social media channels. SMIM has impacted many industries including tourism, but looking at the progressive growth of SMIM today, it is surprising that such little attention had been paid to this area. However, while there is increasing use of SMIM by tourism organizations, there is a lack of research and limited knowledge on the roles of SMIM in travel and tourism. This chapter sheds light on the use of SMIM in the tourism sector where existing literature on the SMIM, its factors, and the influence of SMIM on the tourism sector by distinct authors are reviewed to identify the effectiveness of SMIM in the travel and tourism industry.
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Zineb, Sellal, Ouazzani Touhami Amina, Dahmani Jamila, Maazouzi Soukaina, Mouden Najoua, Chliyeh Mohamed, Selmaoui Karima, Benkirane Rachid, El Modafar Cherkaoui, and Douira Allal. "Diversity of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Argania spinosa in Morocco." In Mycorrhiza - New Insights [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.106162.

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Despite the importance of arbuscular mycorrhizal fungi (AMF) within forest and agroecosystems, few data are available about how AMF communities are structured in the root zone of the argan tree. Some studies have characterized endomycorrhizal fungi population occurring in rhizosphere soils of argan trees grown in southwest of Morocco, numerous sites in this area harbored unexplored communities. The endomycorrhizae diversity of rhizosphere soils collected from 15 argan forest stands located in Lakhssas, Smimou, Ait Baha, Tamanar, Essaouira, Taroudant (Elkodya), Irherm, Guelmim, Imsouane, Anzi, Tiznit, Taghazoute, Ait Melloul, Bouizakarne, and Oulad Teima have revealed the presence of different AMF communities sharing some species but dissimilar AMF community compositions are noted according to sampling time and site. Additionally, the diverse AMF structures detected such as vesicles, arbuscules and hyphae reflect implicitly the germination of AMF propagules in the rhizospheric area of the Argan tree. The pre-evaluation of AMF in the soil through spores’ density can indicate AMF community dynamics, signaling either the adaptability of mycorrhizal symbionts to the local conditions or its decline. In total, 39 morphotypes of endomycorrhizal fungal spores were identified and described, representing seven genera: Glomus (15 species), Scutellospora (3 species), Entrophospora (4 species), Pacispora (2 species), Gigaspora (4 species), Acaulospora (10 species), and Ambispora (1 species). The genus Glomus has a wide occurrence and had the largest number of species. This chapter gives a great overview of the mycorrhizal status of argan trees in their natural habitats of the main Moroccan argan forests.
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"Rattier M 363 Smart J 227, 287 Rattunde M 34 7 Smimov AB 89 Rees G J 263, 267, 355 Sodesawa J 29 Rehm R 339 Sorba L 389 Reznitsky A A 161 Sorokin S V 161 Rhee J-K 275, 279, 283 Stanley C 291 Ritchie D 371, 439 Stanley R P 359, 363,419 Rochat M 371 Steiner T D 49 Rollbiihler N 235 Stenzel R 255 Royo P 359 Stiff-Roberts AD 117 RudraA 183,415 Stintz A 69 Stolz W 105." In Compound Semiconductors 2002, 491. CRC Press, 2003. http://dx.doi.org/10.1201/9781482269109-79.

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Conference papers on the topic "SMIM10"

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Tolkmit, M. S., R. N. Davudova, S. O. Yeresko, and M. I. Airapetov. "Prolonged Alcohol Abuse Changes the Smim20 Gene mRNA Content in the Brain of Rats During Alcohol Withdrawal." In II Международная конференция, посвящеенная 100- летию И.А. Држевецкой. СКФУ, 2022. http://dx.doi.org/10.38006/9612-62-6.2022.301.302.

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Gray, W., D. Coulter, M. Frerking, and P. Encrenaz. "The FIRST/SMIM international collaboration." In 33rd Aerospace Sciences Meeting and Exhibit. Reston, Virigina: American Institute of Aeronautics and Astronautics, 1995. http://dx.doi.org/10.2514/6.1995-401.

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Picazo-Bueno, José Ángel, and Vicente Micó. "SMIM in reflection imaging mode." In Computational Optical Sensing and Imaging. Washington, D.C.: OSA, 2019. http://dx.doi.org/10.1364/cosi.2019.jw2a.2.

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Rubin, K. A., W. Jolley, and Y. Yang. "Finite-Element Modeling and Quantitative Measurement Using Scanning Microwave Microscopy to Characterize Dielectric Films." In ISTFA 2018. ASM International, 2018. http://dx.doi.org/10.31399/asm.cp.istfa2018p0561.

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Abstract Scanning Microwave Impedance Microscopy (sMIM) can be used to characterize dielectric thin films and to quantitatively discern film thickness differences. FEM modeling of the sMIM response provides understanding of how to connect the measured sMIM signals to the underlying properties of the dielectric film and its substrate. Modeling shows that sMIM can be used to characterize a range of dielectric film thicknesses spanning both low-k and medium-k dielectric constants. A model system consisting of SiO2 thin films of various thickness on silicon substrates is used to illustrate the technique experimentally.
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Iyer, D., A. Messinger, R. Crowder, Y. Zhang, O. Amster, S. Friedman, Y. Yang, and F. Stanke. "Measurement of Dielectric Constant and Doping Concentration of a Cross-Sectioned Device by Quantitative Scanning Microwave Impedance Microscopy." In ISTFA 2017. ASM International, 2017. http://dx.doi.org/10.31399/asm.cp.istfa2017p0613.

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Abstract Scanning microwave impedance microscopy (sMIM) is an emerging technique that can provide detailed information beyond that of conventional scanning capacitance microscopy (SCM), and other electrical scanning probe microscopy (SPM) techniques, for the investigation and failure analysis (FA) of semiconductor devices. Integration of new dielectric materials at lower levels of the device structure with the need for quantification of dielectric and dopants in semiconductor devices with sub-micron spatial resolution pushes the practical boundaries of typical atomic force microscopy (AFM) electrical modes. sMIM can measure both linear and non-linear materials (insulators and doped semiconductors, respectively) simultaneously. sMIM has a linear response to log k (dielectric number) and log N (doping concentration) making it an ideal method for providing quantitative measurements of semiconductor devices over a large range of values. This work demonstrates an example of a practical application of sMIM for quantitative measurement of the dopant concentration profile in production semiconductor devices. A planar dopant calibration sample is used to calibrate the sMIM prior to performing the measurements on an “unknown” production device. We utilize nanoscale C-V data to establish a calibration curve for both n- and p-type carriers and apply the calibration curve to an “unknown” device, presenting the measurements in units of doping concentration.
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Friedman, Stuart, Oskar Amster, Yongliang Yang, and Fred Stanke. "Nanoscale Capacitance and Capacitance-Voltage Curves for Advanced Characterization of Electrical Properties of Si and GaN Structures Using Scanning Microwave Impedance Microscopy." In ISTFA 2016. ASM International, 2016. http://dx.doi.org/10.31399/asm.cp.istfa2016p0449.

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Abstract The use of Atomic Force Microscopy (AFM) electrical measurement modes is a critical tool for the study of semiconductor devices and process development. A relatively new electrical mode, scanning microwave impedance microscopy (sMIM), measures a material’s change in permittivity and conductivity at the scale of an AFM probe tip [1]. sMIM provides the real and imaginary impedance (Re(Z) and Im(Z)) of the probe-sample interface. By measuring the reflected microwave signal as a sample of interest is imaged with an AFM, we can in parallel capture the variations in permittivity and conductivity and, for doped semiconductors, variations in the depletion-layer geometry. An existing technique for characterizing doped semiconductors, scanning capacitance microscopy, modulates the tip-sample bias and detects the tip-sample capacitance with a lock-in amplifier. A previous study compares sMIM to SCM and highlights the additional capabilities of sMIM [2], including examples of nano-scale capacitance-voltage curves. In this paper we focus on the detailed mechanisms and capabilities of the nano-scale C-V curves and the ability to extract semiconductor properties from them. This study includes analytical and finite element modeling of tip bias dependent depletion-layer geometry and impedance. These are compared to experimental results on reference samples for both doped Si and GaN doped staircases to validate the systematic response of the sMIM-C (capacitive) channel to the doping concentration.
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Hu, Wei-Shan, Jeng-Han Lee, Ming-Hong Kao, Hui-Wen Yang, Peter De Wolf, and Oskar Amster. "Device Dielectric Quality Analysis and Fault Isolation at the Contact Level by Scanning Microwave Impedance Microscopy." In ISTFA 2016. ASM International, 2016. http://dx.doi.org/10.31399/asm.cp.istfa2016p0463.

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Abstract Dielectric film quality is one of the most important factors that will greatly impact device performance and reliability. Device level electrical analysis techniques for dielectric quality monitoring are highly needed. In this paper we present results using a new electrical AFM mode, scanning Microwave Impedance Microscopy (sMIM), for characterization of device oxide quality and for fault isolation. Devices with poor oxide quality show sMIM nano C-V and dC/dV hysteresis behavior during forward and reverse bias sweep. The sMIM capacitance sensitivity is below 1 aF allowing one to capture C-V spectra from the MOS structure formed by the gate and gate oxide with excellent signal/noise ratio and observe subtle variations between different sites.
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De Wolf, Peter, Zhuangqun Huang, and Bede Pittenger. "Spectroscopy-Based Mapping with Scanning Microwave Impedance Microscopy." In ISTFA 2018. ASM International, 2018. http://dx.doi.org/10.31399/asm.cp.istfa2018p0550.

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Abstract Methods are available to measure conductivity, charge, surface potential, carrier density, piezo-electric and other electrical properties with nanometer scale resolution. One of these methods, scanning microwave impedance microscopy (sMIM), has gained interest due to its capability to measure the full impedance (capacitance and resistive part) with high sensitivity and high spatial resolution. This paper introduces a novel data-cube approach that combines sMIM imaging and sMIM point spectroscopy, producing an integrated and complete 3D data set. This approach replaces the subjective approach of guessing locations of interest (for single point spectroscopy) with a big data approach resulting in higher dimensional data that can be sliced along any axis or plane and is conducive to principal component analysis or other machine learning approaches to data reduction. The data-cube approach is also applicable to other AFM-based electrical characterization modes.
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Dewolf, Peter, and Wanxin Sun. "Multi-Dimensional Nanoscale Characterizations on Devices by DataCube-sMIM." In 2020 IEEE International Symposium on the Physical and Failure Analysis of Integrated Circuits (IPFA). IEEE, 2020. http://dx.doi.org/10.1109/ipfa49335.2020.9260896.

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Antoniou, Nicholas, Ravi Chintala, and Yongliang Yang. "Scanning microwave impedance microscopy (sMIM) for materials characterization and metrology." In Metrology, Inspection, and Process Control for Semiconductor Manufacturing XXXV, edited by Ofer Adan and John C. Robinson. SPIE, 2021. http://dx.doi.org/10.1117/12.2584560.

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Reports on the topic "SMIM10"

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Chernick, C. M. Federal SMIME V3 client profile. Gaithersburg, MD: National Institute of Standards and Technology, 2002. http://dx.doi.org/10.6028/nist.sp.800-49.

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Leiba, B. Creation of a Registry for smime-type Parameter Values. RFC Editor, January 2014. http://dx.doi.org/10.17487/rfc7114.

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