Academic literature on the topic 'SLNPV'

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Journal articles on the topic "SLNPV"

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Sinambela, D. P., D. Bakti, M. C. Tobing, and Y. M. Kusumah. "Molecular characterization of Spodoptera litura Nucleopolyhedrovirus (SlNPV) Labuhan Batu isolate." IOP Conference Series: Earth and Environmental Science 974, no. 1 (January 1, 2022): 012011. http://dx.doi.org/10.1088/1755-1315/974/1/012011.

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Abstract Spodoptera litura F. is a new pest that potentially becomes a serious pest threatening palm oil industry. The control can be carried out using biological control agents S.litura Nucleopolyhedrovirus (SlNPV). Molecular approach using polymerase chain reaction (PCR) has been used to characterize the SlNPV isolate. The aim of this study was to determine the molecular characteristics of SlNPV Labuhan Batu isolate based on DNA sequence analysis. The NPV isolate was collected from oil palm plantation Negeri Lama Estate Group, PT. Hari sawit Jaya, Asian Agri, Labuhan Batu. The SlNPV DNA extraction was conducted using the DNA gSYNC kit. The amplification of SlNPV DNA was done by specific primers with oligonucleotide sequences forward 5′-ACGTTACGATCTAGATCC-3′ and reverse 5′-AACGTTAGACAGACATAT-3′ with ±700bp of amplicon. Homology and phylogeny characteristics were used to determine the relationship of SlNPV isolate from Labuhan Batu to NPV from other countries were reported in the GeneBank of National Center for Biotechnology Information (NCBI). The results showed that SlNPV isolate from Labuhan Batu had the closest related to SlNPV from Australia, SliNPV from Canada and SlNPV from China, with the similarity percentage 100%, 96% and 90% respectively. They belong to the same group as the NPVs that infected the Spodoptera genus.
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Hasanah, Uswatun, and Nanang Tri Haryadi. "EFEKTIVITAS BAHAN PELINDUNG Spodoptera litura NUCLEAR POLYHEDROSIS VIRUS (SlNPV) UNTUK MENGENDALIKAN Spodoptera litura (F.) PADA TANAMAN KEDELAI." Jurnal Hama dan Penyakit Tumbuhan 10, no. 3 (September 16, 2022): 148–55. http://dx.doi.org/10.21776/ub.jurnalhpt.2022.010.3.5.

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Spodoptera litura dapat menyebabkan kehilangan hasil hingga 80% bahkan gagal panen. Nuclear Polyhedrosis Virus merupakan agensi hayati dari kelompok patogen serangga famili Baculoviridae yang banyak digunakan sebagai agens hayati dan Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) merupakan salah satu pemanfaatan NPV yang efektif dalam mengendalikan Spodoptera litura. Kendala penggunaan SlNPV yaitu tidak tahan terhadap sinar matahari sehingga perlu bahan pelindung agar virus tidak mengalami inaktif. Penelitian ini bertujuan untuk mengetahui pengaruh bahan pelindung dalam mempertahankan efektivitas SlNPV dari paparan sinar matahari. Penelitian dilakukan di rumah kaca dengan menggunakan Rancangan Acak Lengkap (RAL) non faktorial. Perlakuan meliputi SlNPV 100 ml tanpa pemaparan UV A (a), SlNPV 100 ml tanpa bahan pelindung + UV A (b), SlNPV 100 ml + Molase 40% + UV A (c), SlNPV 100 ml + Kaolin 40% + UV A (d), SlNPV 100 ml + Tinopal 1% + UV A (e), SlNPV 100 ml + Sunblock SPF 50 5% + UV A (f). Setiap perlakuan diulang sebanyak 4 kali sehingga didapatkan 24 satuan percobaan. Hasil penelitian menunjukkan bahwa pada uji skala rumah kaca perlakuan SlNPV + kaolin 40% dapat menyebabkan mortalitas S. litura 92,5% diikuti oleh perlakuan SlNPV + molase 40% dengan mortalitas sebesar 75% pada pengamatan 9 hari setelah aplikasi. Kaolin efektif digunakan sebagai bahan pelindung SlNPV dibandingkan bahan pelindung lainnya karena dapat melindungi SlNPV dari paparan sinar Ultra Violet (UV) karena membentuk lapisan seperti lapisan film.
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Afidah, Nafisatul, Tutung Hadiastono, Bedjo Bedjo, and Fery Abdul Choliq. "Compatibility of Lemongrass Extract with Spodoptera litura Nuclear Polyhedrosis Virus Against Spodoptera litura on Soybean Plants." Journal of Tropical Plant Protection 2, no. 1 (January 1, 2021): 7–13. http://dx.doi.org/10.21776/ub.jtpp.2021.002.1.2.

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Armyworm (Spodoptera litura) is an important pest that damages the leaves of soybean. SlNPV is one type of pathogen that has potential as a biocontrol agent to control armyworms because it is specific, selective, and effective. This study aimed to investigate the compatibility between the lemongrass and SlNPV in controlling armyworm. The Research was conducted at the Laboratory of Indonesian Legumes and Tuber Crops Research Institute (ILETRI) from February 2016 until April 2016. The study using completely randomized design with 7 treatments and 3 replication with a treatment that consists of control, SlNPV-JTM 97C 1,5 × 106 PIB/ml, 10% of lemongrass extract, a combination SlNPV-JTM 97C fixed concentrate of 1,5 × 106 PIB/ml with 2.5%, 5%, 7.5%, and 10% of lemongrass extract. The results showed that the combination of SlNPV-JTM 97C 1.5 × 106 PIB/ml with 10% lemongrass had the highest level of virulence, with 96% of mortality on larva 100% on the pupa stage, so that the combination of SlNPV and lemongrass extracts was compatible in controlling S.litura.
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Putri, Nahdia, Tri Wardhani, Untung Sugiarti, Toto Suharjanto, and Firman Hidayat. "POTENSI MANGKOKAN (Nothopanax scutellarium) DAN KEMANGI (Ocimum sanctum L.) SEBAGAI UV PROTEKTAN SLNPV JTM 97C." Agrika 15, no. 1 (March 3, 2022): 55. http://dx.doi.org/10.31328/ja.v15i1.3557.

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Spodoptera litura NPV (SlNPV) dapat berfungsi sebagai biopestisida yang digunakan untuk mengendalikan hama tanaman. SlNPV memiliki beberapa kelebihan dalam mengendalikan S. litura yaitu memiliki inang yang spesifik sehingga tidak membahayakan organisme selain hama sasaran maupun lingkungan. Kelemahannya adalah bahwa SlNPV menjadi inaktivasi setelah terpapar sinar ultraviolet (UV). SlNPV mulai kehilangan keefektifan setelah 12 jam terpapar sinar matahari secara langsung dan tingkat patogenitasnya (virulensi) menurun 50%. Upaya meningkatkan keefektifan SlNPV di lapangan dilakukan dengan rekayasa formulasi yaitu menyertakan bahan tambahan (adjuvant) yang dapat melindungi SlNPV dari sinar ultraviolet. Kaolin selama ini digunakan sebagai UV protektan bagi SlNPV JTM 97C karena sifat menghantarkan panasnya rendah tetapi bukan sumber daya alam terbarukan. Penelitian ini dilakukan untuk mencari bahan adjuvant sebagai UV protektan pengganti kaolin. Daun mangkokan dan daun kemangi memiliki potensi sebagai UV protektan karena mengandung senyawa flavonoid dan fenolik yang bermanfaat sebagai antioksidan dan berkhasiat sebagai tabir surya. Penelitian dilakukan secara faktorial dengan faktor pertama UV protektan berupa kaolin, ekstrak daun mangkokan & ekstrak daun kemangi. Faktor perlakuan kedua adalah durasi penyinaran. Hasil penelitian menunjukkan bahwa flavonoid, fenolik dan tanin pada ekstrak daun kemangi secara kualitatif lebih banyak dibanding ekstrak daun mangkokan. Pada 4 jam setelah inokulasi jumlah larva Spodotera litura yang stop feeding pada kombinasi perlakuan UV protektan kaolin yang disinari UV 9 jam lebih tinggi dibanding kombinasi perlakuan ekstrak daun kemangi yang disinari 6 jam, dan juga lebih tinggi dibanding perlakuan ekstrak daun mangkokan yang disinari UV 9 jam. Namun larva S. litura yang mati sampai dengan 10 hari setelah aplikasi tidak berbeda antara UV protektan kaolin, ekstrak daun kemangi dan ekstrak daun mangkokan. ABSTRACTSpodoptera litura NPV (SlNPV) can function as a biopesticide used to control plant pests. SlNPV has several advantages in controlling S. litura, namely specific host so that it does not harm organisms other than the target pest and the environment. The disadvantage is that SlNPV becomes inactivated upon exposure to ultraviolet (UV) light. SlNPV began to lose effectiveness after 12 hours of direct sunlight and decreased its pathogenicity (virulence) by 50%. Efforts to increase the effectiveness of SlNPV in the field are carried out by engineering formulations that include adjuvants that can protect SlNPV from ultraviolet light. Kaolin has been used as a UV protector for SlNPV JTM 97C because of its low heat conductivity, but it is not a renewable natural resource. This research was conducted to find adjuvants as UV protectors to replace kaolin. Mangkokan leaves and basil leaves have potential as UV protectors because they contain flavonoid and phenolic compounds that are useful as antioxidants and are efficacious as sunscreens. The research was carried out in a factorial experiment with the first factor was UV protectors in the form of caolin, mangkokan leaf & basil leaf extract. The second treatment factor is the duration of irradiation. The results showed that the flavonoids, phenolics and tannins in the basil leaf extract were qualitatively more than the mangkokan leaf extract. At 4 hours after inoculation the number of Spodotera litura larvae that stopped feeding in the combination of UV protectant kaolin treatment with 9 hours UV irradiation was higher than the combination of basil leaf extract treatment with 6 hours irradiation, and also higher than that of the mangkokan leaf extract treatment with 9 hours UV irradiation. . However, the larvae of S. litura that died up to 10 days after application did not differ between the UV protectors of kaolin, basil leaf extract and mangkokan leaf extract.
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Du, Quansheng, Dana Lehavi, Ouriel Faktor, Yipeng Qi, and Nor Chejanovsky. "Isolation of an Apoptosis Suppressor Gene of theSpodoptera littoralis Nucleopolyhedrovirus." Journal of Virology 73, no. 2 (February 1, 1999): 1278–85. http://dx.doi.org/10.1128/jvi.73.2.1278-1285.1999.

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ABSTRACT Spodoptera frugiperda SF9 cells infected with mutants of the Autographa californica nucleopolyhedrovirus (AcMNPV) which lack a functional p35 gene undergo apoptosis, aborting the viral infection. The Spodoptera littoralis nucleopolyhedrovirus (SlNPV) was able to suppress apoptosis triggered by vΔP35K/pol+, an AcMNPVp35 null mutant. To identify the putative apoptotic suppressor gene of SlNPV, overlapping cosmid clones representing the entire SlNPV genome were individually cotransfected along with genomic DNA of vΔP35K/pol+. Using this complementation assay, we isolated a SlNPV DNA fragment that was able to rescue the vΔP35K/pol+ infection in SF9 cells. By further subcloning and rescue, we identified a novel SlNPV gene, Slp49. TheSlp49 sequence predicted a 49-kDa polypeptide with about 48.8% identity to the AcMNPV apoptotic suppressor P35. SLP49 displays a potential recognition site, TVTDG, for cleavage by death caspases. Recombinant AcMNPVs deficient inp35 bearing the Slp49 gene did not induce apoptosis and showed successful productive infections in SF9 cells, indicating that Slp49 is a functional homologue ofp35. A 1.5-kbp Slp49-specific transcript was identified in SF9 cells infected with SlNPV or with vAc496, a vΔP35K/pol+-recombinant bearing Slp49. The discovery of Slp49 contributes to the identification of important functional motifs conserved in p35-like apoptotic suppressors and to the future isolation of p35-like genes from other baculoviruses.
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Bayu, Marida Santi Yudha Ika, Yusmani Prayogo, and Gatut Wahyu Anggoro Susanto. "INTEGRATED BIOLOGICAL TECHNOLOGY TO CONTROL MUNGBEAN PESTS AND DISEASES." Indonesian Journal of Agricultural Science 22, no. 1 (July 9, 2021): 8. http://dx.doi.org/10.21082/ijas.v22n1.2021.p8-16.

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The main constraints to increase mungbean production in Indonesia are pests and diseases. The application of integrated biological agents can improve the efficacy of controlling the mungbean pests and diseases. The study aimed to determine the efficacy of integrated biological agents to suppress mungbean pests and diseases. This field research was conducted from May to July 2018 using a randomized block design with seven treatments and four replicates. The treatments were: T1 = Trichol + NSP, T2 = Trichol + SlNPV, T3 = Trichol + NSP + SlNPV, T4 = Trichol + NSP + SlNPV + BeBas, T5 = Trichol + NSP + SlNPV + BeBas + GE, T6 = chemical pesticides, and T7 = control. The results showed that the highest efficacy occurred in T4 and T5 treatments which saved the yield loss from major pests and diseases attack, and did not differ significantly with chemical pesticides (T6). Treatments T4 was able to reduce the development of soil borne diseases by 3% and suppress Spodoptera litura attack by 9.8% as compared to chemical treatment. T4 was also more efficient than T5 because it uses less biological agents. The advantage of biological agents is compatible if they were used together with predators such as Oxyopes sp., Paederus sp. and Coccinella sp; and also Telenomus sp. and Trichogramma sp. parasitoids. On the other hand, the chemical pesticides (T6) killed all existing natural enemies. Therefore, T4 could be recommended for controlling mungbean pests and diseases.
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Syahroni, M. Novel Ghufron, and Nanang Tri Haryadi. "Uji Efektivitas Konsentrasi Spodoptera litura – Nuclear Polyhedrosis Virus (SlNPV) JTM 97C Formulasi Bubuk Terhadap Larva Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Pada Tanaman Kedelai." Jurnal Pengendalian Hayati 2, no. 2 (September 25, 2019): 46. http://dx.doi.org/10.19184/jph.v2i2.17140.

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Spodoptera litura (armyworm) is an important leaf pest that is polyphagic and has a broad range of hosts. Spodoptera litura is the main pest in soybean plants and can cause up to 50% damage and often results in decreased productivity and even crop failure. Spodoptera litura is widespread in areas with hot and humid climates from the subtropics to the tropics. S. litura pests attack cultivation plants in the vegetative and generative phases. The prospect of insect pathogens as a substitute for chemical insecticides is quite good in terms of their effectiveness and impact on the environment. Nuclear Polyhedrosis Virus (NPV) is one of the Baculoviridae viruses that infect the larval stage. Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) is a pathogenic virus that infects armyworms. SlNPV JTM 97C is an effective isolate in controlling larvae of Spodoptera litura. The larvae used in this study were larval instar 4, instar 5, and instar 6. The study was conducted using a Completely Randomized Design (CRD) with 6 treatments and 3 replications. The treatments consisted of controls, 1 g / l, 2g / l, 3 g / l, 4 g / l, and 5 g / l. Each treatment was applied to instar 4, instar 5 and 6 instar larvae. The results showed that SlNPV JTM 97C affected mortality, stopped eating and pupa formation of instar 4, instar 5 larvae, but did not affect instar 6 larvae. The younger the larval instar the higher the pathogenicity response.
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MASUDA, Toshio, Hidenori IWAHANA, and Kisaku AKUTU. "Studies on transovum transmission of the nuclear polyhedrosis virus of Spodoptera litura (SiNPV). I. Infection of SlNPV to the next generation larvae through the male moths contaminated with SlNPV." Japanese journal of applied entomology and zoology 34, no. 1 (1990): 1–6. http://dx.doi.org/10.1303/jjaez.34.1.

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Lu, Liqun, Quansheng Du, and Nor Chejanovsky. "Reduced Expression of the Immediate-Early Protein IE0 Enables Efficient Replication of Autographa californica Multiple Nucleopolyhedrovirus in Poorly Permissive Spodoptera littoralis Cells." Journal of Virology 77, no. 1 (January 1, 2003): 535–45. http://dx.doi.org/10.1128/jvi.77.1.535-545.2003.

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ABSTRACT Infection of Spodoptera littoralis SL2 cells with the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) results in apoptosis and low yields of viral progeny, in contrast to infection with S. littoralis nucleopolyhedrovirus (SlNPV). By cotransfecting SL2 cells with AcMNPV genomic DNA and a cosmid library representing the complete SlNPV genome, we were able to rescue AcMNPV replication and to isolate recombinant virus vAcSL2, which replicated efficiently in SL2 cells. Moreover, vAcSL2 showed enhanced infectivity for S. littoralis larvae compared to AcMNPV. The genome of vAcSL2 carried a 519-bp insert fragment that increased the distance between the TATA element and the transcriptional initiation site (CAGT) of immediate-early gene ie0. This finding correlated with low steady-state levels of IE0 and higher steady-state levels of IE1 (the product of the ie1 gene, a major AcMNPV transactivator, and a multifunctional protein) than of IE0. Mutagenesis of the ie0 promoter locus by insertion of the chloramphenical acetyltransferase (cat) gene yielded a new recombinant AcMNPV with replication properties identical to those of vAcSL2. Thus, the analysis indicated that increasing the steady-state levels of IE1 relative to IE0 should enable AcMNPV replication in SL2 cells. This suggestion was confirmed by constructing a recombinant AcMNPV bearing an extra copy of the ie1 gene under the control of the Drosophila hsp70 promoter. These results suggest that IE0 plays a role in the regulation of AcMNPV infection and show, for the first time, that significant improvement in the ability of AcMNPV to replicate in a poorly permissive cell line and organism can be achieved by increasing the expression of the main multiple functional protein, IE1.
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AHMED, YASMEIN E., HEBA YOUSEF, and SHIMAA M. DESOKY. "EFFECTS OF MELIA AZEDARACH METHANOL EXTRACT AND NUCLEOPOLYHEDROVIRUS (SLNPV) ON SPODOPTERA LITTORALIS (BOISD.)." Egyptian Journal of Agricultural Research 92, no. 2 (May 15, 2014): 513–26. http://dx.doi.org/10.21608/ejar.2014.155173.

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Dissertations / Theses on the topic "SLNPV"

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Tahir, Hanan Ismail Mohamed. "Tritrophic relationships between entomopathogens, lepidopteran crop pests and host plant factors using Spodoptera littoralis (Boisd.), SLNPV, Bacillus thuringiensis Berliner and Beauveria bassiana Balsamo as model systems." Thesis, University of Reading, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.397837.

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Biswakarma, Dipen. "Development of Stimuli-Responsive Self-Assembled Materials for Biomolecular Applications." Thesis, 2019. https://etd.iisc.ac.in/handle/2005/4567.

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The thesis entitled ‘‘Development of Stimuli-Responsive Self-Assembled Materials for Biomolecular Applications’’ deals with the design, synthesis, and characterisation of low molecular weight hydrogel systems as well as small molecular probes and their responses towards various stimuli. Depending upon the nature of their association, the present work has been divided into two main subsections. Chapter 1 presents an overview of the general area of the low molecular weight hydrogel and stimuli-responsive hydrogel, different types of gelator molecules, their self-assembly patterns, along with applications of the LMOGs hydrogels. Chapter 2A deals with the synthesis of pyrene-based monosaccharides namely D-gluconic acid and glucose and their gelation propensity. Selective gelation of pyrene linked gluconic acid-based amphiphilic compound, both in aqueous medium and EtOH. Further, the gelator was utilized to differentiate between alcoholic and non-alcoholic beverages and the sensing of insulin. Chapter 2B describes the synthesis and self-assembly properties of two different pyrene-disaccharide compounds (with maltose and lactose) in an aqueous medium. Lactose based Hydrogel is successfully utilized for studying the sustainable release of anticancer drugs, such as Doxorubicin (DOX) and mitoxantrone (MT). In addition, lactose-based amphiphile also showed specific interaction with Cholera Toxin B (CTB). Chapter 3A. describes the self-assembly studies as well as hydrogelation of a tyrosine-functionalized low molecular weight pyrenyl probe (L-PyTyrOH) in a buffered medium (pH 7.4). Then lanthanide ion (Eu3+) is doped into the hydrogel network and the resultant metallogel is used for the detection of pathogenic biomarkers such as dipicolinic (DPA) acid and phytic acid at physiological pH. Chapter 3B describes the formation of a two-component ‘charge transfer’ (CT) hydrogel system, comprising of a pyrene-amino acid conjugate and a 4-Chloro-7-nitrobenzofurazan (NBD) derivative in 1:1 molar ratio. CT gel was used for the ‘Turn-on’ sensing (cyan emission) of the biogenic amino acids, namely cysteine and homocysteine Chapter 4A. describes the design and self-assembly studies of the pyrene-based amphiphilic compounds having different amino acids residues at the terminal position, such as histidine and tryptophan. Moreover, the present system was also used for detecting the α-chymotrypsin in a human blood serum sample Chapter 4B. describes the synthesis and self-assembly studies of the of gallate attached pyrenyl derivatives connected via aromatic amino acid namely phenylalanine and tyrosine. The hydrogels were then involved in designing sensor array for Cu2+, Hg2+, and Fe3+ and selective sensing of hypochlorite Chapter 4C. deals with the studies of different hydrogel-nanocomposite hybrid materials where graphene oxide has been involved as carbon nanomaterial. The hybrid hydrogels are utilized for the removal of organic dye namely methylene Blue (MB) and rhodamine B (RhB) from the aqueous solution and spermine estimation. Chapter 5A presents the design and synthesis of a ruthenium complex-based charge transfer probe and its application in selective detection of Spodoptera Litura Nuclear Polyhedrosis Virus (SLNPV). Chapter 5B describes the ratiometric sensing of the Spilosoma Obliqua Nuclear Polyhedrosis Virus (SONPV) by acetyl ester derivative of pyranine dye.
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Books on the topic "SLNPV"

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Eberlinc, Nataša. Greh kamnitih slnov. Trbovlje: samozal, 2009.

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Conference papers on the topic "SLNPV"

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Carvalho, Hugo E. T., Natalia C. Fernandes, Otto Carlos M. B. Duarte, and Guy Pujolle. "SLAPv: A service level agreement enforcer for virtual networks." In 2012 International Conference on Computing, Networking and Communications (ICNC). IEEE, 2012. http://dx.doi.org/10.1109/iccnc.2012.6167514.

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Mbarek, Nader, Francine Krief, and Daniel Negru. "SLNP usage for QoS negotiation in heterogeneous environments." In 2008 IEEE/ACS International Conference on Computer Systems and Applications (AICCSA). IEEE, 2008. http://dx.doi.org/10.1109/aiccsa.2008.4493660.

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Chalouf, M. A., X. Delord, and F. Krief. "Introduction of Security in the Service Level Negotiated with SLNP Protocol." In 2008 New Technologies, Mobility and Security (NTMS). IEEE, 2008. http://dx.doi.org/10.1109/ntms.2008.ecp.53.

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Zhang, Guiyang, Jiashan Cui, Yunhui Li, and Yinglong Li. "Semi-time-varying H∞ controller for SLPV systems with modal persistent dwell time." In 2016 35th Chinese Control Conference (CCC). IEEE, 2016. http://dx.doi.org/10.1109/chicc.2016.7553311.

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