Relevant bibliographies by topics / SiRNA

Academic literature on the topic 'SiRNA'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 May 2024

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Journal articles on the topic "SiRNA"

1

Rodriguez-Salazar, Carlos Andrés, Delia Piedad Recalde-Reyes, Juan Pablo Bedoya, Leonardo Padilla-Sanabria, Jhon Carlos Castaño-Osorio, and Maria Isabel Giraldo. "In Vitro Inhibition of Replication of Dengue Virus Serotypes 1–4 by siRNAs Bound to Non-Toxic Liposomes." Viruses 14, no. 2 (February 7, 2022): 339. http://dx.doi.org/10.3390/v14020339.

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Dengue virus is a ssRNA+ flavivirus, which produces the dengue disease in humans. Currently, no specific treatment exists. siRNAs regulate gene expression and have been used systematically to silence viral genomes; however, they require controlled release. Liposomes show favorable results encapsulating siRNA for gene silencing. The objective herein was to design and evaluate in vitro siRNAs bound to liposomes that inhibit DENV replication. siRNAs were designed against DENV1–4 from conserved regions using siDirect2.0 and Web-BLOCK-iT™ RNAiDesigner; the initial in vitro evaluation was carried out through transfection into HepG2 cells. siRNA with silencing capacity was encapsulated in liposomes composed of D-Lin-MC3-DMA, DSPC, Chol. Cytotoxicity, hemolysis, pro-inflammatory cytokine release and antiviral activity were evaluated using plaque assay and RT-qPCR. A working concentration of siRNA was established at 40 nM. siRNA1, siRNA2, siRNA3.1, and siRNA4 were encapsulated in liposomes, and their siRNA delivery through liposomes led to a statistically significant decrease in viral titers, yielded no cytotoxicity or hemolysis and did not stimulate release of pro-inflammatory cytokines. Finally, liposomes were designed with siRNA against DENV, which proved to be safe in vitro.
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Vijayaraghavan, Bhooma, Giri Padmanabhan, and Kumaresan Ramanathan. "Assessment of siRNA as a therapeutic molecule in Transient Receptor Potential Channel 5 gene silencing: a computational approach." Biomedical Research and Therapy 5, no. 1 (January 19, 2018): 1911–22. http://dx.doi.org/10.15419/bmrat.v5i1.405.

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Background: Ion channels play a crucial role in Glomerular filter damage that contributes to albuminuria. Transient receptor potential channel 5 (TRPC5) gene mediating such damage, demand for its target specific inhibition by RNA interference mechanism. Designing and selecting potential siRNA for TRPC5 gene silencing by computational analysis. Materials & Methods: The mRNA sequence was retrieved from NCBI (National Center for Biotechnology Information). siRNA sequences were designed specifically from target genes using InvivoGen siRNA wizard software. Thermodynamic RNA-RNA interactions were used to evaluate the gene silencing efficiency by minimum free energy of hybridization; the hybridization structures were also obtained using BIBISERV2-RNAHybrid. Results: The minimum free energy of hybridization of the three designed siRNAs (siRNA1, siRNA2 and siRNA3) were as follows: -28.2 kcal/mol, -24.1 kcal/mol, and-25.6 kcal/mol. Their corresponding GC content were 47.62%, 52.38% and 47.62%, respectively. Thus, siRNA1 had the least minimum free energy of hybridization (i.e. -28.2 kcal/mol) with low GC content (47.62%), and high linearity with minimal h-b index and loop structure. Conclusion: RNAi therapy can provide a new platform for efficient and targeted therapeutics. Further in vivo investigations are necessary to further validate their efficacy.
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Yu, Ji-wei, Shou-lian Wang, Ju-gang Wu, Rui-qi Lu, Xiao-chun Ni, Cheng Cai, and Bo-jian Jiang. "Study on the Biological Characteristics of CD133+ Cells Interfered by RNA Interference in Gastric Cancer." ISRN Gastroenterology 2014 (March 19, 2014): 1–11. http://dx.doi.org/10.1155/2014/329519.

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Background. To detect the changes of biological characteristics in gastric cancer cells interfered by CD133-specific small interfering RNA (siRNA). Methods. First to select the siRNA which has the strongest interference effect among 3 siRNAs (i.e., siRNA1, siRNA2, and siRNA3) in KATO-III cells by RT-PCR and Western blotting assays. Then, CD133+ cells were sorted out from KATO-III cells using an immunomagnetic bead sorting method and transfected with the selected siRNA. Furthermore, the proliferating characteristics, the antichemotherapeutic assessment, Transwell invasion assay, monoclonal sphere formation assay, and subcutaneous transplanted tumor formation assay in nude mice were investigated. Results. siRNA3 showed the strongest interference effect in KATO-III cells. As compared to the uninterfered control group, the CD133+ cells treated by siRNA3 showed significant decreases in the abilities of proliferation, invasion, clone sphere formation, and resistance to antitumour drugs as well as the weight and size of the transplanted tumor, which was nearly similar to that of CD133− cells. Additionally, the protein expression level of the EMT factor E-cadherin increased while those of EMT-related Snail and N-cadherin decreased in CD133+ cells interfered by siRNA3. Conclusion. Inhibition of CD133 gene expression reduces the abilities of gastric cancer cells in proliferation, invasion, clonal sphere formation, and chemoresistance as well as tumor formation in nude mice.
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Kadhim, Kadhim Kamil, Anas Y. Al-Hayawi, and Fawzyia A. R. Ibrahim. "The role of siRNA in inhibition the biofilm formation as a first line of antibiotic resistance by regulation the MsrA drug efflux pump in Staphylococcus saprophyticus." International journal of health sciences 6, S1 (March 20, 2022): 1336–44. http://dx.doi.org/10.53730/ijhs.v6ns1.4904.

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The bacterium Staphylococcus saprophyticus (S. saprophyticus) is a common cause of urinary tract infections (UTI) in the community. To determine the effect of siRNA on the mRNA of the msrA gene in S. saprophyticus, 21-23 bp small interfering RNA (siRNA) duplexes were constructed against the mRNA of the msrA gene. The effect of siRNA on msrA mRNA expression was determined using reverse transcription PCR (RT-PCR). To assess changes in biofilm formation (BF) in response to siRNA activity, the usual tube technique was adopted. In vitro, msrA-siRNAs inhibited S. saprophyticus mRNA expression and activity. The efficacy of siRNA was determined by comparing the BF in S. saprophyticus before and after msrA-siRNA was introduced into the bacteria. In this investigation, two msrA-siRNA sequences, siRNA1 and siRNA2, were employed. qRT-PCR revealed that two msrA-siRNA sequences significantly suppressed the expression of msrA-mRNA, P = (0.010 and 0.002 ) respectively at (P< 0.05) comparison with control. Regarding the BF results after treatment with two siRNA sequences, they were 5/6 (83.5%) and 4/6 (67%) negative formation and 1/6 (16.5%) and 2/6 (33%) positive formation, respectively compared with control.
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Shmushkovich, Taisia, Kathryn R. Monopoli, Diana Homsy, Dmitriy Leyfer, Monica Betancur-Boissel, Anastasia Khvorova, and Alexey D. Wolfson. "Functional features defining the efficacy of cholesterol-conjugated, self-deliverable, chemically modified siRNAs." Nucleic Acids Research 46, no. 20 (August 29, 2018): 10905–16. http://dx.doi.org/10.1093/nar/gky745.

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Abstract Progress in oligonucleotide chemistry has produced a shift in the nature of siRNA used, from formulated, minimally modified siRNAs, to unformulated, heavily modified siRNA conjugates. The introduction of extensive chemical modifications is essential for conjugate-mediated delivery. Modifications have a significant impact on siRNA efficacy through interference with recognition and processing by RNAi enzymatic machinery, severely restricting the sequence space available for siRNA design. Many algorithms available publicly can successfully predict the activity of non-modified siRNAs, but the efficiency of the algorithms for designing heavily modified siRNAs has never been systematically evaluated experimentally. Here we screened 356 cholesterol-conjugated siRNAs with extensive modifications and developed a linear regression-based algorithm that effectively predicts siRNA activity using two independent datasets. We further demonstrate that predictive determinants for modified and non-modified siRNAs differ substantially. The algorithm developed from the non-modified siRNAs dataset has no predictive power for modified siRNAs and vice versa. In the context of heavily modified siRNAs, the introduction of chemical asymmetry fully eliminates the requirement for thermodynamic bias, the major determinant for non-modified siRNA efficacy. Finally, we demonstrate that in addition to the sequence of the target site, the accessibility of the neighboring 3′ region significantly contributes to siRNA efficacy.
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Guo, Yong, Hongyan Guo, Liang Zhang, Hongying Xie, Xin Zhao, Fangxun Wang, Ze Li, et al. "Genomic Analysis of Anti-Hepatitis B Virus (HBV) Activity by Small Interfering RNA and Lamivudine in Stable HBV-Producing Cells." Journal of Virology 79, no. 22 (November 15, 2005): 14392–403. http://dx.doi.org/10.1128/jvi.79.22.14392-14403.2005.

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ABSTRACT Hepatitis B virus (HBV) causes acute and chronic hepatitis and hepatocellular carcinoma. Small interfering RNA (siRNA) and lamivudine have been shown to have anti-HBV effects through different mechanisms. However, assessment of the genome-wide effects of siRNA and lamivudine on HBV-producing cell lines has not been reported, which may provide a clue to interrogate the HBV-cell interaction and to evaluate the siRNA's side effect as a potential drug. In the present study, we designed seven siRNAs based on the conserved HBV sequences and tested their effects on the expression of HBV genes following sorting of siRNA-positive cells. Among these seven siRNAs, siRNA-1 and siRNA-7 were found to effectively suppress HBV gene expression. We further addressed the global gene expression changes in stable HBV-producing cells induced by siRNA-1 and siRNA-7 by use of human genome-wide oligonucleotide microarrays. Data from the gene expression profiling indicated that siRNA-1 and siRNA-7 altered the expression of 54 and 499 genes, respectively, in HepG2.2.15 cells, which revealed that different siRNAs had various patterns of gene expression profiles and suggested a complicated influence of siRNAs on host cells. We further observed that 18 of these genes were suppressed by both siRNA-1 and siRNA-7. Interestingly, seven of these genes were originally activated by HBV, which suggested that these seven genes might be involved in the HBV-host cell interaction. Finally, we have compared the effects of siRNA and lamivudine on HBV and host cells, which revealed that siRNA is more effective at inhibiting HBV expression at the mRNA and protein level in vitro, and the gene expression profile of HepG2.2.15 cells treated by lamivudine is totally different from that seen with siRNA.
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Bolhassani, Azam, and Alireza Milani. "Small Interfering RNAs and their Delivery Systems: A Novel Powerful Tool for the Potential Treatment of HIV Infections." Current Molecular Pharmacology 13, no. 3 (July 9, 2020): 173–81. http://dx.doi.org/10.2174/1874467212666191023120954.

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: Small interfering RNAs (siRNAs) have rapidly developed into biomedical research as a novel tool for the potential treatment of various human diseases. They are based on altered gene expression. In spite of the availability of highly active antiretroviral therapy (HAART), there is a specific interest in developing siRNAs as a therapeutic agent for human immunodeficiency virus (HIV) due to several problems including toxicity and drug resistance along with long term treatment. The successful use of siRNAs for therapeutic goals needs safe and effective delivery to specific cells and tissues. Indeed, the efficiency of gene silencing depends on the potency of the carrier used for siRNA delivery. The combination of siRNA and nano-carriers is a potent method to prevent the limitations of siRNA formulation. Three steps were involved in non-viral siRNA carriers such as the complex formation of siRNA with a cationic carrier, conjugation of siRNA with small molecules, and encapsulation of siRNA within nanoparticles. : In this mini-review, the designed siRNAs and their carriers are described against HIV-1 infections both in vitro and in vivo.
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Wang, Xuan, and Fa Zhang. "Prediction of siRNA Efficacy Using BP Neural Network and Support Vector Machine." Applied Mechanics and Materials 701-702 (December 2014): 214–18. http://dx.doi.org/10.4028/www.scientific.net/amm.701-702.214.

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RNA interference (RNAi) is a mechanism for sequence-specific, post-transcriptional down-regulation of gene expression. The success of RNAi gene silencing depends on siRNA feature design. The shortcoming of previously reported methods which design siRNA sequences based on limited rules is that they are difficult to accurately predict the efficacy that a candidate siRNA sequence will silence the target gene. With validated siRNA databases have been developed in recent years, machine learning methods can be applied to predict siRNA accuracy and optimize design. This paper proposed a combined prediction method of BP neural network and support vector machine (SVM) for selecting effective siRNA sequences. With SVM, siRNA sequences were classified into effective or ineffective siRNAs. Subsequently, BP neural network model with great learning ability selected highly effective candidate sequences from effective siRNAs. We applied this method to published siRNAs datasets, and the experimental results confirmed good prediction capability.
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Chernikov, Ivan V., Ulyana A. Ponomareva, and Elena L. Chernolovskaya. "Structural Modifications of siRNA Improve Its Performance In Vivo." International Journal of Molecular Sciences 24, no. 2 (January 4, 2023): 956. http://dx.doi.org/10.3390/ijms24020956.

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The use of small interfering RNA (siRNA) in the clinic gives a wide range of possibilities for the treatment of previously incurable diseases. However, the main limitation for biomedical applications is their delivery to target cells and organs. Currently, delivery of siRNA to liver cells is a solved problem due to the bioconjugation of siRNA with N-acetylgalactosamine; other organs remain challenging for siRNA delivery to them. Despite the important role of the ligand in the composition of the bioconjugate, the structure and molecular weight of siRNA also play an important role in the delivery of siRNA. The basic principle is that siRNAs with smaller molecular weights are more efficient at entering cells, whereas siRNAs with larger molecular weights have advantages at the organism level. Here we review the relationships between siRNA structure and its biodistribution and activity to find new strategies for improving siRNA performance.
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Rajeswaran, Rajendran, Victor Golyaev, Jonathan Seguin, Anna S. Zvereva, Laurent Farinelli, and Mikhail M. Pooggin. "Interactions of Rice Tungro Bacilliform Pararetrovirus and Its Protein P4 with Plant RNA-Silencing Machinery." Molecular Plant-Microbe Interactions® 27, no. 12 (December 2014): 1370–78. http://dx.doi.org/10.1094/mpmi-07-14-0201-r.

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Small interfering RNA (siRNA)-directed gene silencing plays a major role in antiviral defense. Virus-derived siRNAs inhibit viral replication in infected cells and potentially move to neighboring cells, immunizing them from incoming virus. Viruses have evolved various ways to evade and suppress siRNA production or action. Here, we show that 21-, 22-, and 24-nucleotide (nt) viral siRNAs together constitute up to 19% of total small RNA population of Oryza sativa plants infected with Rice tungro bacilliform virus (RTBV) and cover both strands of the RTBV DNA genome. However, viral siRNA hotspots are restricted to a short noncoding region between transcription and reverse-transcription start sites. This region generates double-stranded RNA (dsRNA) precursors of siRNAs and, in pregenomic RNA, forms a stable secondary structure likely inaccessible to siRNA-directed cleavage. In transient assays, RTBV protein P4 suppressed cell-to-cell spread of silencing but enhanced cell-autonomous silencing, which correlated with reduced 21-nt siRNA levels and increased 22-nt siRNA levels. Our findings imply that RTBV generates decoy dsRNA that restricts siRNA production to the structured noncoding region and thereby protects other regions of the viral genome from repressive action of siRNAs, while the viral protein P4 interferes with cell-to-cell spread of antiviral silencing.
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Dissertations / Theses on the topic "SiRNA"

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Edinger, Daniel. "siRNA therapy for cancer." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-158123.

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Gotthardt, Carl Martin [Verfasser], and Regine [Akademischer Betreuer] Süss. "Entwicklung und Charakterisierung von LAH4-L1-Peptid-Protamin-siRNA-Partikeln zum siRNA-Transport." Freiburg : Universität, 2017. http://d-nb.info/1141053462/34.

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Chen, Chao. "Amphiphilic dendrimers for siRNA delivery." Thesis, Aix-Marseille, 2015. http://www.theses.fr/2015AIXM4738.

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Le défi majeur de la thérapie génique à base de siARN est sa délivrance sûre et efficace. Récemment, notre groupe a mis au point des dendrimères amphiphiles comme vecteurs robustes et efficaces de délivrance non-virale de siARN, qui combinent les avantages de délivrance des vecteurs lipidiques et polymèriques. J’ai effectué au cours de ma thèse de doctorat une analyse de la relation structure/activité (SAR) d'une série de dendrimères comportant des queues hydrophobes de différentes longueurs. Nos résultats démontrent qu’un équilibre optimal entre la longueur de la chaîne alkyle hydrophobe et la partie hydrophile dendritique joue un rôle crucial sur leur capacité d’auto-assemblage, ainsi que sur leur activité de transport des siRNA. En outre, en combinant bola-amphiphiles et nos dendrimères amphiphiles, nous avons développé un nouveau dendrimère bola-amphiphile dont nous avons étudié les propriétés d’auto-assemblage et l'efficacité de transport du siARN correspondant. Ce dendrimère bola-amphiphile particulier a été en mesure de réagir à des espèces réactives de l'oxygène pour la délivrance spécifique, ouvrant ainsi de nouvelles perspectives pour la conception de vecteurs stimuli-déclencheurs pour siARN ciblés. Enfin, nous avons étudié l’«effet d'éponge à protons» des vecteurs dendritiques amphiphiles à l'aide de la technique du film Langmuir en monocouche. Nos résultats ont prouvé le gonflement des vecteurs dendritiques amphiphiles par protonation, offrant ainsi des données expérimentales permettant de soutenir sans ambiguïté l’hypothèse de l'«effet d'éponge à protons»
A key challenge in RNAi-based gene therapy is the safe and effective siRNA delivery. Recently, our group has established amphiphilic dendrimers as robust and effective nonviral delivery vectors for siRNA, which combine the beneficial delivery features of both lipid and dendritic polymer vectors while overcoming their shortcomings.With the desire to understand the underlying mechanism of amphiphilic dendrimers for efficient delivery, I performed a structure/activity relationship (SAR) analysis of a series of dendrimers featuring hydrophobic tails of different lengths during my PhD thesis. We systematically investigated these dendrimers for their self-assembling characters and their capacities for both binding and delivery of siRNA. Our results demonstrate that an optimal balance between the hydrophobic alkyl chain length and the hydrophilic dendritic portion plays a crucial role in the self-assembly and the delivery activity towards siRNA.Furthermore, we developed a novel bola-amphiphilic dendrimer by combining bola-amphiphiles and our amphiphilic dendrimers and studied their self-assembly properties and the corresponding siRNA delivery efficiency. This peculiar bola-amphiphilic vector was able to respond to reactive oxygen species for specific delivery, opening a new perspective for the design of stimuli-trigged vectors for targeted siRNA delivery.Finally, I studied the “proton sponge effect” of the amphiphilic dendrimer vectors using the Langmuir monolayer film technique. Our results gave direct evidence of swelling of the amphiphilic dendrimers upon protonation, offering unambiguous experimental data to support the “proton sponge effect”
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Chen, Chao. "Amphiphilic dendrimers for siRNA delivery." Electronic Thesis or Diss., Aix-Marseille, 2015. http://www.theses.fr/2015AIXM4738.

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Le défi majeur de la thérapie génique à base de siARN est sa délivrance sûre et efficace. Récemment, notre groupe a mis au point des dendrimères amphiphiles comme vecteurs robustes et efficaces de délivrance non-virale de siARN, qui combinent les avantages de délivrance des vecteurs lipidiques et polymèriques. J’ai effectué au cours de ma thèse de doctorat une analyse de la relation structure/activité (SAR) d'une série de dendrimères comportant des queues hydrophobes de différentes longueurs. Nos résultats démontrent qu’un équilibre optimal entre la longueur de la chaîne alkyle hydrophobe et la partie hydrophile dendritique joue un rôle crucial sur leur capacité d’auto-assemblage, ainsi que sur leur activité de transport des siRNA. En outre, en combinant bola-amphiphiles et nos dendrimères amphiphiles, nous avons développé un nouveau dendrimère bola-amphiphile dont nous avons étudié les propriétés d’auto-assemblage et l'efficacité de transport du siARN correspondant. Ce dendrimère bola-amphiphile particulier a été en mesure de réagir à des espèces réactives de l'oxygène pour la délivrance spécifique, ouvrant ainsi de nouvelles perspectives pour la conception de vecteurs stimuli-déclencheurs pour siARN ciblés. Enfin, nous avons étudié l’«effet d'éponge à protons» des vecteurs dendritiques amphiphiles à l'aide de la technique du film Langmuir en monocouche. Nos résultats ont prouvé le gonflement des vecteurs dendritiques amphiphiles par protonation, offrant ainsi des données expérimentales permettant de soutenir sans ambiguïté l’hypothèse de l'«effet d'éponge à protons»
A key challenge in RNAi-based gene therapy is the safe and effective siRNA delivery. Recently, our group has established amphiphilic dendrimers as robust and effective nonviral delivery vectors for siRNA, which combine the beneficial delivery features of both lipid and dendritic polymer vectors while overcoming their shortcomings.With the desire to understand the underlying mechanism of amphiphilic dendrimers for efficient delivery, I performed a structure/activity relationship (SAR) analysis of a series of dendrimers featuring hydrophobic tails of different lengths during my PhD thesis. We systematically investigated these dendrimers for their self-assembling characters and their capacities for both binding and delivery of siRNA. Our results demonstrate that an optimal balance between the hydrophobic alkyl chain length and the hydrophilic dendritic portion plays a crucial role in the self-assembly and the delivery activity towards siRNA.Furthermore, we developed a novel bola-amphiphilic dendrimer by combining bola-amphiphiles and our amphiphilic dendrimers and studied their self-assembly properties and the corresponding siRNA delivery efficiency. This peculiar bola-amphiphilic vector was able to respond to reactive oxygen species for specific delivery, opening a new perspective for the design of stimuli-trigged vectors for targeted siRNA delivery.Finally, I studied the “proton sponge effect” of the amphiphilic dendrimer vectors using the Langmuir monolayer film technique. Our results gave direct evidence of swelling of the amphiphilic dendrimers upon protonation, offering unambiguous experimental data to support the “proton sponge effect”
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Harder, Johannes. "Synthese und Anwendung dendritischer siRNA Strukturen." Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-168635.

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Serginson, James Michael. "Bio-reducible polyamines for siRNA delivery." Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/14688.

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Even though siRNA shows great promise in the treatment of genetic disease, cancer and viral infection; the lack of a suitable delivery vector remains a barrier to clinical use. Currently, viral vectors lead the field in terms of efficacy but are generally regarded as prohibitively dangerous. Synthetic alternatives such as cationic polymers could overcome this problem. Previous work in the group found that small, cationic, disulfide-containing, cyclic polyamines – despite being non-polymeric – were useful as vectors for pDNA transfection; this work focuses on adapting the material for siRNA. A branched analogue of the cyclic compounds was prepared and the synthetic procedures investigated are discussed. The suitability of both compounds for siRNA delivery was studied in depth. Characterisation of their interactions with nucleic acids under various conditions was carried out using light-scattering techniques, gel electrophoresis and fluorescent dye exclusion assays. Results from these experiments were used to allow successful use of the materials as vectors and enable understanding of the mechanism of the template-driven polymerisation. Early data concerning the efficacy of the materials as an siRNA delivery system in vitro was obtained using A549 lung carcinoma cells as a model system with siRNAs targeting the production of the enzyme GAPDH. Both compounds showed a hint of successful siRNA Delivery but the data was not overwhelmingly conclusive. Further experiments will be required to optimise the materials for maximum biological efficacy and to confirm they offer potential as a novel delivery system.
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Patikarnmonthon, Nisa. "PMPC-PDPA polymersomes-mediated siRNA delivery." Thesis, University of Sheffield, 2014. http://etheses.whiterose.ac.uk/5476/.

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Polymersomes made from the amphiphilic diblock copolymers, PMPC-PDPA, are proposed to serve as a siRNA carrier with pH-responsive property that provides endosomal escape. The main purpose of this work is to investigate the ability of polymersomes to provide effective intracellular delivery of siRNA into HeLa cells. Encapsulation of siRNA into polymersomes was performed by pH-switch and electroporation method, both techniques enable siRNA encapsulation. No alteration of polymersomes size and morphology was observed in DLS and TEM. Purification of polymersome was conducted to ensure that no free siRNA or polymer remained. Intracellular delivery was examined by using fluorescence-labelled siRNA to track the internalisation. Flow cytometry and fluorescence microscope were used to study the cellular uptake of polymersomes and siRNA. siRNA is successfully delivered with the distribution of siRNA signal throughout the cell, with stronger signal compared with Lipofectamine. Kinetic uptake of siRNA suggests that siRNA can be effectively delivered to most cells within 20 hours. In addition, evidence of endosomal escape of siRNA delivered by polymersomes was observed. Silencing activity of siRNA was determined by qPCR and Western blot, mRNA and protein expression of Lamin A/C as a target gene were not significantly decreased. Cytotoxicity and other cellular response, including pro-inflammatory response and interferon response, were investigated. Polymersomes provide very low cytotoxicity and no pro-inflammatory response, unlike Lipofectamine. Moreover, the gene expression profile of interferon response indicates the possible apoptosis occurrence in Lipofectamine treated cells, but not in polymersomes treated cells. The information suggests two possible factors that influence the silencing activity of siRNA delivered by polymersomes; the incomplete characterisation of siRNA process and the cellular response from carriers.
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Mui, Yuen-chi. "Comparison and improvement of siRNA design tools." Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B30722019.

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Willibald, Julian. "Anandamid-vermittelte Aufnahme von siRNA in Immunzellen." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-159962.

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Mui, Yuen-chi, and 梅宛芝. "Comparison and improvement of siRNA design tools." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B30722019.

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Books on the topic "SiRNA"

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Taxman, Debra J., ed. siRNA Design. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-119-6.

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Shum, Kato, and John Rossi, eds. SiRNA Delivery Methods. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3112-5.

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Hasan, Mustapa. Dangdanggula sirna rasa. Bandung: Kiblat, 2009.

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Sioud, Mouldy. Ribozymes and siRNA protocols. New Jersey: Humana Press, 2004. http://dx.doi.org/10.1385/1592597467.

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Mouldy, Sioud, ed. Ribozymes and siRNA protocols. 2nd ed. Totowa, N.J: Humana Press, 2004.

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Sioud, Mouldy, ed. siRNA and miRNA Gene Silencing. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-547-7.

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Firman, Abdul. Sinar yang tidak pernah sirna. [Jakarta]: Yayasan TRIP 4000 Besuki, 1997.

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Bulaa, Daggafaa. Maalummaa heeraafi sirna mootummaa federaalawaa. [Addis Ababa: s.n.], 2010.

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Ditzel, Henrik J., Martina Tuttolomondo, and Sakari Kauppinen, eds. Design and Delivery of SiRNA Therapeutics. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1298-9.

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Zaki, Siti Hajar Mohd. Sirna sebutir bintang: Kumpulan cerpen remaja. Kuala Lumpur: Dewan Bahasa dan Pustaka, 2004.

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Book chapters on the topic "SiRNA"

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Arnemann, J. "siRNA." In Springer Reference Medizin, 2173. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_3580.

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Nakatsura, Tetsuya. "SiRNA." In Encyclopedia of Cancer, 1–5. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-27841-9_5324-3.

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Morgan, Michael M., MacDonald J. Christie, Luis De Lecea, Jason C. G. Halford, Josee E. Leysen, Warren H. Meck, Catalin V. Buhusi, et al. "siRNA." In Encyclopedia of Psychopharmacology, 1238–39. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-68706-1_1065.

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Arnemann, J. "siRNA." In Lexikon der Medizinischen Laboratoriumsdiagnostik, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-49054-9_3580-1.

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Nakatsura, Tetsuya. "SiRNA." In Encyclopedia of Cancer, 4221–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-662-46875-3_5324.

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Nakatsura, Tetsuya. "SiRNA." In Encyclopedia of Cancer, 3413–16. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-16483-5_5324.

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Zhu, Yimei, Hiromi Inada, Achim Hartschuh, Li Shi, Ada Della Pia, Giovanni Costantini, Amadeo L. Vázquez de Parga, et al. "siRNA Delivery." In Encyclopedia of Nanotechnology, 2429. Dordrecht: Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-90-481-9751-4_100766.

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Salem, Aliasger K., and Mark A. Behlke. "SiRNA Delivery Vehicles." In RNA Interference, 174–215. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470923733.ch6.

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Hughes, Jeffrey, Preeti Yadava, and Ryan Mesaros. "Liposomal siRNA Delivery." In Methods in Molecular Biology, 445–59. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-360-2_31.

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Wright, R. Scott. "PCSK9 Inhibiting siRNA." In Stroke Revisited: Dyslipidemia in Stroke, 135–43. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-3923-4_12.

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Conference papers on the topic "SiRNA"

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Bachkova, I. K., S. A. Zhukov, M. S. Kupryushkin, M. A. Zenkova, E. L. Chernolovskaya, and I. V. Chernikov. "THE EFFECT OF MESYL PHOSPHORAMIDATE MODIFICATION ON SIRNA POTENCY IN VITRO." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-295.

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The introduction of chemical modifications into the composition of small interfering RNA (siRNA) improves its biological properties, but can inhibit the RNA interference (RNAi) process. Therefore, in this work, we screened the patterns of modification of siRNAs with mesyl groups (μ), covering 70% of positions in siRNAs. In most positions, μ did not inhibit the RNAi process, so the introduction of μ can potentially improve its biological properties in vivo.
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Kornev, A. A., V. V. Vysochinskaya, N. A. Knyazev, A. K. Emel'yanov, and A. A. Bogdanov. "Transfection of human peripheral blood T-lymphocytes with synthetic small interfering RNAs: selection of an effective technique." In Global science. Development and novelty. L-Journal, 2020. http://dx.doi.org/10.18411/gsdn-25-12-2020-04.

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It has been shown that the state of the human immune system and its activity determines the development, course and prognosis of many oncological diseases. To date, various immunotherapeutic approaches have been developed, of which the most promising is adoptive cell therapy (ACT). An increase in the effectiveness of this type of therapy could be achieved by using synthetic small interfering RNAs (siRNAs) to suppress the expression of key immune checkpoint (ICI) genes (PD1, CTL4) in T-lymphocytes, which are involved in cellular immunosuppression. However, there is a problem of selecting an effective method for delivering such siRNAs to T-lymphocytes. In the present study, we developed a synthetic siRNA specific to the mRNA sequence of the PD1 gene and evaluated the efficiency of its delivery to activated human T-lymphocytes using chemically modified siRNA, histone H1, – 18 – Global science. Development and novelty and the liposomal agent HiperFect. Ex vivo activated T-lymphocytes from healthy donors were used. The efficiency of siRNA transfection into cells and its confirmation was assessed using flow cytofluorometry and confocal microscopy. As a result of the study, a high (51%) efficiency of transfection into these cells using chemically modified "self-delivering" siRNA was shown. For other methods of delivery of miRNA to T-lymphocytes, low efficiency is shown. Thus, our data suggest that of the three approaches used in this work for miRNAs delivery to activated T-lymphocytes, the most effective is the use of “self-delivering” chemically modified cholesterol miRNA.
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Zhao, Wenzhong, and Terran Lane. "siRNA off-target search." In the 5th international workshop. New York, New York, USA: ACM Press, 2005. http://dx.doi.org/10.1145/1134030.1134040.

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Dongfang Wang, Xiang Chen, Fa Zhang, Peizhuo Zhang, Guohua Liu, and Xin Guo. "A Method to Improve the Universality of SiRNA Design Rules Based on siRNA Efficiency Distribution." In 2008 International Symposium on Information Science and Engineering (ISISE). IEEE, 2008. http://dx.doi.org/10.1109/isise.2008.284.

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Vysochinskaya, V. V., Y. A. Zabrodskaya, O. V. Dovbysh, M. A. Egorova, and M. A. Maslov. "CELL-PENETRATING PEPTIDE AND CATIONIC LIPOSOMES MEDIATED SIRNA DELIVERY TO ARREST GROWTH OF CHRONIC MYELOID LEUKEMIA CELLS IN VITRO." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-305.

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Comparison of the cell-penetrating peptide EB1 and original 2X3-DOPE-PEG cationic liposomes for anti-BCR-ABL siRNA delivery into cells of chronic myeloid leukemia showed that 2X3-DOPE-PEG cationic liposomes deliver siRNA more efficiently into K562 cells, that leads to more effective inhibition of expression of the targeted gene and cancer cell proliferation.
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Xiaohu Gao. "Traceable siRNA delivery with quantum dots." In 2009 Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2009. http://dx.doi.org/10.1109/iembs.2009.5334549.

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Duman Scheel, Molly. "siRNA larvicides for control of vector mosquitoes." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.94203.

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Sardo, Carla, Carmela Tommasino, Tiziana Esposito, Giulia Auriemma, and Rita Patrizia Aquino. "SIRNA Delivery Mediated By Inulin Based Polycations." In The 8th World Congress on Recent Advances in Nanotechnology. Avestia Publishing, 2023. http://dx.doi.org/10.11159/nddte23.127.

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Aliabadi, Hamidreza Montazeri, Parvin Mahdipoor, Cezary Kucharsky, Nicole Chan, and Hasan Uludag. "Abstract A57: Effects of pre-exposure to siRNA on silencing response: Do cells become resistant to siRNA silencing?" In Abstracts: AACR Precision Medicine Series: Drug Sensitivity and Resistance: Improving Cancer Therapy; June 18-21, 2014; Orlando, FL. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1557-3265.pms14-a57.

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Qiu, Shibin, and Terran Lane. "The RNA String Kernel for siRNA Efficacy Prediction." In 2007 IEEE 7th International Symposium on BioInformatics and BioEngineering. IEEE, 2007. http://dx.doi.org/10.1109/bibe.2007.4375581.

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Reports on the topic "SiRNA"

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Medina-Kauwe, Lali K. Targeting Sirna Missiles to Her2+ Breast Cancer. Fort Belvoir, VA: Defense Technical Information Center, June 2008. http://dx.doi.org/10.21236/ada486880.

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Medina-Kauwe, Lali K. Targeting siRNA Missiles to Her2+ Breast Cancer. Fort Belvoir, VA: Defense Technical Information Center, June 2009. http://dx.doi.org/10.21236/ada517257.

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Medina-Kauwe, Lali K. Targeting siRNA Missiles to HER2+ Breast Cancer. Fort Belvoir, VA: Defense Technical Information Center, June 2007. http://dx.doi.org/10.21236/ada472023.

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Joyce, Christine, and Deidre Mountain. Optimization of Liposomal Encapsulation Efficiency. University of Tennessee Health Science Center, 2021. http://dx.doi.org/10.21007/com.lsp.2018.0002.

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Introduction: My project was a continuation of the Vascular Research Lab’s (VRL) ongoing research at the University of Tennessee Medical Center Knoxville (UTMCK) aimed at optimizing liposomal encapsulation efficiency of small interfering RNA (siRNA) which can be used to silence genes to prevent a variety of disease pathologies. Methods: Assay siRNA loading capacity of liposomes based on lipid concentration Development of a method for liposome purification: HPLC & HiTRAP Column Results & Conclusion: siRNA loading capacity Higher lipid:siRNA resulted in increased encapsulation efficiency HPLC – did not work as expected HiTRAP Column – currently being optimized to be used as part of standard operating procedures
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Lichy, Jack H. Inducible siRNA Vectors for Probing Signaling Pathways in Breast Cancer Cells. Fort Belvoir, VA: Defense Technical Information Center, September 2004. http://dx.doi.org/10.21236/ada432997.

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O'Keefe, Regis J. Development of siRNA Technology to Prevent Scar Formation in Tendon Repair. Fort Belvoir, VA: Defense Technical Information Center, October 2012. http://dx.doi.org/10.21236/ada575075.

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O'Keefe, Regis J. Development of siRNA Technology to Prevent Scar Formation in Tendon Repair. Fort Belvoir, VA: Defense Technical Information Center, December 2013. http://dx.doi.org/10.21236/ada598989.

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Gal-On, Amit, Shou-Wei Ding, Victor P. Gaba, and Harry S. Paris. role of RNA-dependent RNA polymerase 1 in plant virus defense. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7597919.bard.

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Objectives: Our BARD proposal on the impact of RNA-dependent RNA polymerase 1 (RDR1) in plant defense against viruses was divided into four original objectives. 1. To examine whether a high level of dsRNA expression can stimulate RDR1 transcription independent of salicylic acid (SA) concentration. 2. To determine whether the high or low level of RDR1 transcript accumulation observed in virus resistant and susceptible cultivars is associated with viral resistance and susceptibility. 3. To define the biogenesis and function of RDR1-dependent endogenous siRNAs. 4. To understand why Cucumber mosaic virus (CMV) can overcome RDR1-dependent resistance. The objectives were slightly changed due to the unique finding that cucumber has four different RDR1 genes. Background to the topic: RDR1 is a key plant defense against viruses. RDR1 is induced by virus infection and produces viral and plant dsRNAs which are processed by DICERs to siRNAs. siRNAs guide specific viral and plant RNA cleavage or serve as primers for secondary amplification of viral-dsRNA by RDR. The proposal is based on our preliminary results that a. the association of siRNA and RDR1 accumulation with multiple virus resistance, and b. that virus infection induced the RDR1-dependent production of a new class of endogenous siRNAs. However, the precise mechanisms underlying RDR1 induction and siRNA biogenesis due to virus infection remain to be discovered in plants. Major conclusions, solutions and achievements: We found that in the cucurbit family (cucumber, melon, squash, watermelon) there are 3-4 RDR1 genes not documented in other plant families. This important finding required a change in the emphasis of our objectives. We characterized 4 RDR1s in cucumber and 3 in melon. We demonstrated that in cucumber RDR1b is apparently a new broad spectrum virus resistance gene, independent of SA. In melon RDR1b is truncated, and therefore is assumed to be the reason that melon is highly susceptible to many viruses. RDR1c is dramatically induced due to DNA and RNA virus infection, and inhibition of RDR1c expression led to increased virus accumulation which suggested its important on gene silencing/defense mechanism. We show that induction of antiviral RNAi in Arabidopsis is associated with production of a genetically distinct class of virus-activated siRNAs (vasiRNAs) by RNA dependent RNA polymerase-1 targeting hundreds of host genes for RNA silencing by Argonaute-2. Production of vasiRNAs is induced by viruses from two different super groups of RNA virus families, targeted for inhibition by CMV, and correlated with virus resistance independently of viral siRNAs. We propose that antiviral RNAi activate broad-spectrum antiviral activity via widespread silencing of host genes directed by vasiRNAs, in addition to specific antiviral defense Implications both scientific and agricultural: The RDR1b (resistance) gene can now be used as a transcription marker for broad virus resistance. The discovery of vasiRNAs expands the repertoire of siRNAs and suggests that the siRNA-processing activity of Dicer proteins may play a more important role in the regulation of plant and animal gene expression than is currently known. We assume that precise screening of the vasiRNA host targets will lead in the near future for identification of plant genes associate with virus diseases and perhaps other pathogens.
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Chakraborty, Srijani. The Dawn of RNA Therapeutics. Spring Library, December 2020. http://dx.doi.org/10.47496/sl.blog.19.

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Li, Benyi. Inhibition of Androgen-Independent Growth of Prostate Cancer by siRNA-Mediated Androgen Receptor Gene Silencing. Fort Belvoir, VA: Defense Technical Information Center, February 2005. http://dx.doi.org/10.21236/ada435609.

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