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Journal articles on the topic 'Single Strand Conformation Polymorphism'

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Published: 4 June 2021
Last updated: 1 February 2022

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1

Maréchal, V. "SSCP (single strand conformation polymorphism)." EMC - Biologie médicale 3, no. 1 (January 2008): 1–5. http://dx.doi.org/10.1016/s2211-9698(08)71401-8.

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2

Kaczanowski, Radoslaw, Lech Trzeciak, and Krzysztof Kucharczyk. "Multitemperature single-strand conformation polymorphism." ELECTROPHORESIS 22, no. 16 (September 2001): 3539–45. http://dx.doi.org/10.1002/1522-2683(200109)22:16<3539::aid-elps3539>3.0.co;2-t.

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3

Jedlicka, Anne E., and Steven R. Kleeberger. "Single-Strand Conformation Polymorphism Analysis." Cold Spring Harbor Protocols 2006, no. 1 (June 2006): pdb.prot4118. http://dx.doi.org/10.1101/pdb.prot4118.

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4

Castellv�-Bel, S., A. S�nchez, C. Badenas, J. Mallolas, A. Barcel�, D. Jim�nez, M. Villa, X. Estivill, and M. Mil�. "Single-strand conformation polymorphism analysis in theFMR1." American Journal of Medical Genetics 84, no. 3 (May 28, 1999): 262–65. http://dx.doi.org/10.1002/(sici)1096-8628(19990528)84:3<262::aid-ajmg18>3.0.co;2-v.

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5

Fujita, K., and J. Silver. "Single-strand conformational polymorphism." Genome Research 4, no. 3 (December 1, 1994): S137—S140. http://dx.doi.org/10.1101/gr.4.3.s137.

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6

Krizkova, L., R. Sakthivel, S. A. Olowe, P. K. Rogan, and J. Floros. "Human SP-A: genotype and single-strand conformation polymorphism analysis." American Journal of Physiology-Lung Cellular and Molecular Physiology 266, no. 5 (May 1, 1994): L519—L527. http://dx.doi.org/10.1152/ajplung.1994.266.5.l519.

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We have previously characterized two surfactant protein A (SP-A) cDNAs termed 1A and 6A, as well as a 6A allelic variant termed 6A1. These sequences are quite heterogeneous at the 3' untranslated region (3'UT). Differences between 6A and 6A1 alleles include an 11-bp insertion/deletion 407 bases downstream from the start of the translation termination codon and a base pair polymorphism (C or G) in exon 1 (position 1,193; White, Damm, Miller, Spratt, Schilling, Hawgood, Benson, and Cordell. Nature Lond. 317: 361–363, 1985). The 11-bp (GCCCACTGCCT) segment is present in 6A1 and absent in 6A. The 6A/6A genotype, in a small number of specimens, showed a trend toward a higher frequency in the black Nigerian population compared with Caucasians. In this report, we examine the frequency of the 6A genotype in a larger number of samples from Caucasians and black Nigerians as well as the meiotic stability of the 3'UT heterogeneity. Slot-blot analysis and allele-specific oligonucleotide probes have confirmed that the 6A/6A genotype is more frequent in the Nigerian population. Single-strand conformation polymorphisms in the 3'UT appear to be stably inherited by members of a three-generation family, suggesting that these nucleotide variants represent natural polymorphisms in the population.
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7

Satoh, T., M. Kobayashi, M. Kaneda, M. Tanihiro, K. Okada, and K. Ueda. "Genotypical classification of neutrophil Fc gamma receptor III by polymerase chain reaction-single-strand conformation polymorphism." Blood 83, no. 11 (June 1, 1994): 3312–15. http://dx.doi.org/10.1182/blood.v83.11.3312.3312.

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Abstract We classified the genotype of neutrophil Fc gamma receptor III (FcRIII) (CD16) with a new method. Genomic DNA from mononuclear cells of 39 unrelated healthy donors (13 NA1/NA1, 13 NA2/NA2, and 13 NA1/NA2 typed serologically) were subjected to polymerase chain reaction (PCR) to amplify the polymorphic third exon of the FcRIII genes. The PCR products were heat denatured, electrophoresed, and visualized by silver staining. Allelic differences were detected by distinctive electrophoretic patterns of each single strand, depending on their sequence specific conformations (single-strand conformation polymorphism [SSCP]). The genotypes of neutrophil FcRIII determined by this method were consistent with the phenotypes of NA antigens determined by serologic examinations. These results indicate that the PCR-SSCP system is a very useful tool for genotyping of the neutrophil FcRIII.
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8

Satoh, T., M. Kobayashi, M. Kaneda, M. Tanihiro, K. Okada, and K. Ueda. "Genotypical classification of neutrophil Fc gamma receptor III by polymerase chain reaction-single-strand conformation polymorphism." Blood 83, no. 11 (June 1, 1994): 3312–15. http://dx.doi.org/10.1182/blood.v83.11.3312.bloodjournal83113312.

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We classified the genotype of neutrophil Fc gamma receptor III (FcRIII) (CD16) with a new method. Genomic DNA from mononuclear cells of 39 unrelated healthy donors (13 NA1/NA1, 13 NA2/NA2, and 13 NA1/NA2 typed serologically) were subjected to polymerase chain reaction (PCR) to amplify the polymorphic third exon of the FcRIII genes. The PCR products were heat denatured, electrophoresed, and visualized by silver staining. Allelic differences were detected by distinctive electrophoretic patterns of each single strand, depending on their sequence specific conformations (single-strand conformation polymorphism [SSCP]). The genotypes of neutrophil FcRIII determined by this method were consistent with the phenotypes of NA antigens determined by serologic examinations. These results indicate that the PCR-SSCP system is a very useful tool for genotyping of the neutrophil FcRIII.
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9

Burri, Nathalie, and Pascal Chaubert. "Complex Methylation Patterns Analyzed by Single-Strand Conformation Polymorphism." BioTechniques 26, no. 2 (February 1999): 232–34. http://dx.doi.org/10.2144/99262bm10.

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10

Blanché, Hélène, Christel Valette, and Christine Bellanné-Chantelot. "Optimization of Nonisotopic PCR–Single-Strand Conformation Polymorphism Analysis." Clinical Chemistry 43, no. 11 (November 1, 1997): 2190–92. http://dx.doi.org/10.1093/clinchem/43.11.2190.

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11

Iizuka, M., K. Hayashi, and T. Sekiya. "Single-strand conformation polymorphism (SSCP) at the D8S86 locus." Nucleic Acids Research 19, no. 22 (1991): 6346. http://dx.doi.org/10.1093/nar/19.22.6346.

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12

Lee, James Chun-I., Hsing-Mei Hsieh, Hsiao-Feng Teng, Sheng-Chiang Lo, Adrian Linacre, and Li-Chin Tsai. "ABO genotyping by single strand conformation polymorphism - using CE." ELECTROPHORESIS 30, no. 14 (July 28, 2009): 2544–48. http://dx.doi.org/10.1002/elps.200800815.

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13

Lizuka, M., K. Hayashi, and T. Sekiya. "Single-strand conformation polymorphism (SSCP) at the D8S86 locus." Nucleic Acids Research 20, no. 2 (1992): 388. http://dx.doi.org/10.1093/nar/20.2.388.

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14

AL-ADHAMI, BATOL H., FLORENCE HUBY-CHILTON, BURTON W. BLAIS, AMALIA MARTINEZ-PEREZ, NEIL B. CHILTON, and ALVIN A. GAJADHAR. "Rapid Discrimination of Salmonella Isolates by Single-Strand Conformation Polymorphism Analysis." Journal of Food Protection 71, no. 10 (October 1, 2008): 1960–66. http://dx.doi.org/10.4315/0362-028x-71.10.1960.

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A molecular typing technique was developed for the differentiation of Salmonella isolates based on single-strand conformation polymorphism (SSCP) analysis of amplicons generated by PCR. Amplicons from parts of the fimA (both the 5′ and 3′ ends), mdh, invA, and atpD genes were generated separately from a panel of Salmonella strains representing Salmonella bongori, and four subspecies and 17 serovars of Salmonella enterica. These amplicons were subjected to SSCP analysis for differentiation of the salmonellae on the basis of different conformational forms arising due to nucleotide sequence variations in the target genes. Several distinct SSCP banding patterns (a maximum of 14 each for atpD and fimA 3′ end) were observed with this panel of Salmonella strains for amplicons generated from each target gene. The best discrimination of Salmonella subspecies and serovar was achieved from the SSCP analysis of a combination of at least three gene targets: atpD, invA, and either mdh or fimA 3′ end. This demonstrates the applicability of SSCP analysis as an important additional method to classical typing approaches for the differentiation of foodborne Salmonella isolates. SSCP is simple to perform and should be readily transferable to food microbiology laboratories with basic PCR capability.
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15

Ren, Jicun. "High-throughput single-strand conformation polymorphism analysis by capillary electrophoresis." Journal of Chromatography B: Biomedical Sciences and Applications 741, no. 2 (May 2000): 115–28. http://dx.doi.org/10.1016/s0378-4347(00)00090-6.

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16

Förnzler, Dorothee, Helen Her, Ela W. Knapik, Matthew Clark, Hans Lehrach, John H. Postlethwait, Leonard I. Zon, and David R. Beier. "Gene Mapping in Zebrafish Using Single-Strand Conformation Polymorphism Analysis." Genomics 51, no. 2 (July 1998): 216–22. http://dx.doi.org/10.1006/geno.1998.5386.

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17

Widjojoatmodjo, M. N., A. C. Fluit, and J. Verhoef. "Rapid identification of bacteria by PCR-single-strand conformation polymorphism." Journal of Clinical Microbiology 32, no. 12 (1994): 3002–7. http://dx.doi.org/10.1128/jcm.32.12.3002-3007.1994.

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18

Arakawa, Hidetoshi, Shuji Nakashiro, Masako Maeda, and Akio Tsuji. "Analysis of single-strand DNA conformation polymorphism by capillary electrophoresis." Journal of Chromatography A 722, no. 1-2 (January 1996): 359–68. http://dx.doi.org/10.1016/0021-9673(95)00430-0.

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19

Sekiya, Takao. "Detection of mutant sequences by single-strand conformation polymorphism analysis." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 288, no. 1 (July 1993): 79–83. http://dx.doi.org/10.1016/0027-5107(93)90209-x.

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20

Vallian, Sadeq, and Isar Nassiri. "Development of a Sensitive Deaminated Single-Strand Conformation Polymorphism (DSSCP)." Applied Biochemistry and Biotechnology 160, no. 3 (March 31, 2009): 927–31. http://dx.doi.org/10.1007/s12010-009-8595-y.

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21

Bannai, Makoto, Toshio Mazda, Katsushi Tokunaga, and Takeo Juji. "DNA single-strand conformation polymorphism method to distinguish DR4 alleles." Lancet 341, no. 8847 (March 1993): 769. http://dx.doi.org/10.1016/0140-6736(93)90551-q.

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22

Tokue, Yutaka, Kokichi Sugano, Takeshi Noda, Daizo Saito, Yukio Shimosato, Hisanao Ohkura, Tadao Kakizoe, and Takao Sekiya. "Identification of mycobacteria by nonradioisotopic single-strand conformation polymorphism analysis." Diagnostic Microbiology and Infectious Disease 23, no. 4 (December 1995): 129–33. http://dx.doi.org/10.1016/0732-8893(95)00198-0.

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23

Gasser, Robin B., Xingquan Zhu, and Wayne Woods. "GenotypingTaenia tapeworms by single-strand conformation polymorphism of mitochondrial DNA." Electrophoresis 20, no. 14 (October 1, 1999): 2834–37. http://dx.doi.org/10.1002/(sici)1522-2683(19991001)20:14<2834::aid-elps2834>3.0.co;2-f.

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24

Tisza, Ákos, Ádám Csikós, Ádám Simon, Gabriella Gulyás, András Jávor, and Levente Czeglédi. "Identification of poultry species using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) methods." Food Control 59 (January 2016): 430–38. http://dx.doi.org/10.1016/j.foodcont.2015.06.006.

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25

Nakabayashi, Y., and K. Nishigaki. "Single-Strand Conformation Polymorphism (SSCP) Can Be Explained by Semistable Conformation Dynamics of Single-Stranded DNA." Journal of Biochemistry 120, no. 2 (August 1, 1996): 320–25. http://dx.doi.org/10.1093/oxfordjournals.jbchem.a021416.

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26

MUGGIA, Lucia, and Martin GRUBE. "Fungal composition of lichen thalli assessed by single strand conformation polymorphism." Lichenologist 42, no. 4 (June 3, 2010): 461–73. http://dx.doi.org/10.1017/s0024282909990752.

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AbstractFungi that are unrelated to the mycobiont species frequently colonize lichens. Some of these fungal colonists are described lichenicolous fungi, lichen parasites and pathogens that produce recognizable morphological characters, while others apparently produce no noticeable structures. Here we apply the single strand conformation polymorphism (SSCP) technique to directly assess the abundance of different fungi in lichens. Twenty-eight lichen thalli were chosen, some with and some without externally visible symptoms of parasite infection, and these were subjected to total DNA extraction. PCR was conducted with fungal-specific primers for the ITS region of ribosomal DNA. Single strands of the products were separated on native acrylamide gels. The majority of lichen specimens, both infected and those without symptoms, displayed more than one band in the stained gels. In one case, 14 bands were detected using SSCP. Some of these bands apparently represent other neighbouring lichens in the habitat, but many are apparently non-lichen-forming. Since few lichen-associated fungi have been cultured and sequenced, it is difficult to know if SSCP bands represent obligate lichenicolous fungi, other asymptomatic lichen parasites, or fungi not obligately associated with lichens, but our results indicate that large numbers of non-lichen-forming fungi commonly co-occur with lichens in nature. For specimens of the filamentous lichens Cystocoleus ebeneus and Racodium rupestre we used cloned sequences to compare the number of sequences obtained by the SSCP method to the number obtained by direct sequencing of thallus extracts, and we generally found that more sequences could be detected by SSCP than could be seen by direct sequencing.
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27

Bandyopadhyay, S., A. K. Bera, S. Sikdar, S. De, S. Das, T. Rana, D. Pan, S. Bandyopadhyay, and D. Bhattacharya. "Intra-species variability in ITS-1 sequences of Haemonchus contortus isolated from goats in West Bengal, India." Journal of Helminthology 85, no. 2 (August 31, 2010): 204–9. http://dx.doi.org/10.1017/s0022149x10000465.

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AbstractThis study evaluated the existence of different genotypes of Haemonchus contortus prevailing among goats in West Bengal, India. These parasites were isolated from the abomasum of goat intestine and the molecular characterization was performed by comparing variation of nucleotide sequences of the internal transcribed spacer 1 (ITS-1) gene region. Single-strand conformation polymorphism (SSCP) analysis of ITS-1 amplified product showed the presence of three distinct conformations both in male and female parasites. The sequence analysis of conformations showed two single nucleotide polymorphisms (SNP) in male parasites at nucleotide positions 106 and 107 and one SNP was detected in female parasites at nucleotide position 157. These nucleotide variations in different isolates did not alter the interior loop structure of the predicted secondary RNA, therefore we believe these variations may not be responsible for any evolutionary changes among conformations.
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28

Isam, Zahraa, Rabab Omran Al-jelawi-, and Ammad Hassan Mahmood. "DETECTION SINGLE NUCLEOTIDE POLYMORPHISMS IN UROMODULIN PROMOTER REGION ASSOCIATED WITH RENAL DISEASES USING SINGLE-STRAND CONFORMATION POLYMORPHISM-POLYMERASE CHAIN POLYMORPHISMS TECHNIQUE." Asian Journal of Pharmaceutical and Clinical Research 11, no. 1 (January 1, 2018): 205. http://dx.doi.org/10.22159/ajpcr.2017.v11i1.22063.

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Objective: The uromodulin, a glycoprotein, expressed and secreted by epithelial kidney cells lining the thick ascending limb of the Henle’s loop. It is encoded by the UMOD gene in humans. Our objective was to analyze single nucleotide polymorphisms (SNPs) in the UMOD promoter region in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD).Methods: The blood samples were collected from 100 patients with CKD (50) and ESRD (50), who admitted at Merjan Teaching Hospital in Babylon Province, Iraq (February-July 2016). In addition, 50 blood samples of healthy control. The SNPs of UMOD promoter region was investigated using single-strand conformation polymorphism-polymerase chain polymorphisms (SSCP-PCR) and DNA sequencing techniques.Results: UOMD promoter region polymorphisms using PCR-SSCP and sequencing DNA appeared three different conformational haplotypes, including A\G 49 haplotype (5 bands), A\G 49 and C\A 247 haplotype (5 bands), and C\G 45 and A\G 49 haplotype (6 bands) distributed among CKD and ESRD cases, due to the presence of three SNPs. There was no association between band numbers of PCR-SSCP with ESRD and CKD compared with a control group.Conclusion: SSCP-PCR is a good screening method to detect genetic variations in an uromodulin promoter region.
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29

Isam, Zahraa, Rabab Omran Al-jelawi-, and Ammad Hassan Mahmood. "DETECTION SINGLE NUCLEOTIDE POLYMORPHISMS IN UROMODULIN PROMOTER REGION ASSOCIATED WITH RENAL DISEASES USING SINGLE-STRAND CONFORMATION POLYMORPHISM-POLYMERASE CHAIN POLYMORPHISMS TECHNIQUE." Asian Journal of Pharmaceutical and Clinical Research 11, no. 1 (January 1, 2018): 205. http://dx.doi.org/10.22159/ajpcr.2018.v11i1.22063.

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Objective: The uromodulin, a glycoprotein, expressed and secreted by epithelial kidney cells lining the thick ascending limb of the Henle’s loop. It is encoded by the UMOD gene in humans. Our objective was to analyze single nucleotide polymorphisms (SNPs) in the UMOD promoter region in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD).Methods: The blood samples were collected from 100 patients with CKD (50) and ESRD (50), who admitted at Merjan Teaching Hospital in Babylon Province, Iraq (February-July 2016). In addition, 50 blood samples of healthy control. The SNPs of UMOD promoter region was investigated using single-strand conformation polymorphism-polymerase chain polymorphisms (SSCP-PCR) and DNA sequencing techniques.Results: UOMD promoter region polymorphisms using PCR-SSCP and sequencing DNA appeared three different conformational haplotypes, including A\G 49 haplotype (5 bands), A\G 49 and C\A 247 haplotype (5 bands), and C\G 45 and A\G 49 haplotype (6 bands) distributed among CKD and ESRD cases, due to the presence of three SNPs. There was no association between band numbers of PCR-SSCP with ESRD and CKD compared with a control group.Conclusion: SSCP-PCR is a good screening method to detect genetic variations in an uromodulin promoter region.
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30

Doi, Kent, Hitomi Doi, Eisei Noiri, Akihide Nakao, Toshiro Fujita, and Katsushi Tokunaga. "High-throughput single nucleotide polymorphism typing by fluorescent single-strand conformation polymorphism analysis with capillary electrophoresis." ELECTROPHORESIS 25, no. 6 (March 2004): 833–38. http://dx.doi.org/10.1002/elps.200305721.

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31

Maekawa, Masato, Kayoko Sudo, Dilip Chandra Dey, Kazuo Kotani, and Takashi Kanno. "Effect of electrophoretic conditions on single strand conformation polymorphism (SSCP) pattern." SEIBUTSU BUTSURI KAGAKU 38, no. 2 (1994): 95–101. http://dx.doi.org/10.2198/sbk.38.95.

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32

Sekiya, Takao. "Detection of nucleotide sequence changes by single-strand conformation polymorphism analysis." SEIBUTSU BUTSURI KAGAKU 45, no. 4 (2001): 205–14. http://dx.doi.org/10.2198/sbk.45.205.

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33

Ozawa, Satoshi, Kokichi Sugano, Tsuyoshi Sonehara, Shin'ichi Fukuzono, Akira Ichikawa, Noriko Fukayama, Mariko Taylor, Yuji Miyahara, and Takashi Irie. "High Resolution for Single-Strand Conformation Polymorphism Analysis by Capillary Electrophoresis." Analytical Chemistry 76, no. 20 (October 2004): 6122–29. http://dx.doi.org/10.1021/ac049385k.

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34

King, Stephanie, Bruce R. McCord, and R. Guy Riefler. "Capillary electrophoresis single-strand conformation polymorphism analysis for monitoring soil bacteria." Journal of Microbiological Methods 60, no. 1 (January 2005): 83–92. http://dx.doi.org/10.1016/j.mimet.2004.08.014.

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35

Olivares-Fuster, O., M. Hernandez-Garrido, J. Guerri, and L. Navarro. "Plant somatic hybrid cytoplasmic DNA characterization by single-strand conformation polymorphism." Tree Physiology 27, no. 6 (June 1, 2007): 785–92. http://dx.doi.org/10.1093/treephys/27.6.785.

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36

Sasaki, Yoshitoshi, Nori Nakayashiki, Kiyoshi Saigusa, Masataka Takamiya, and Yasuhiro Aoki. "An application of PCR-single strand conformation polymorphism to MN genotyping." Legal Medicine 2, no. 3 (October 2000): 171–74. http://dx.doi.org/10.1016/s1344-6223(00)80020-6.

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37

Mora, B., F. Petronzelli, R. Grillo, P. Ferrante, and M. C. Mazzilli. "SINGLE-STRAND CONFORMATION POLYMORPHISM (SSCP) ANALYSIS OF HLA-DRB1*1101-06." International Journal of Immunogenetics 23, no. 5 (October 1996): 345–52. http://dx.doi.org/10.1111/j.1744-313x.1996.tb00007.x.

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38

Mohabeer, Ajay J., Alan L. Hiti, and John W. Martin. "Non-radioactive single strand conformation polymorphism (SSCP) using the Pharmacia ‘PhastSystem’." Nucleic Acids Research 19, no. 11 (1991): 3154. http://dx.doi.org/10.1093/nar/19.11.3154.

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39

Sugano, Kokichi, Noriko Fukayama, Hisanao Ohkura, Yukio Shimosato, Yasushi Yamada, Tamotsu Inoue, Takao Sekiya, and Kenshi Hayashi. "Single-strand conformation polymorphism analysis by perpendicular temperature-gradient gel electrophoresis." Electrophoresis 16, no. 1 (1995): 8–10. http://dx.doi.org/10.1002/elps.1150160103.

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40

HIDAYAT, TOPIK, and ADI PANCORO. "Single Strand Conformation Polymorphism Method for Initial Detection DNA Sequences Homogeneity." HAYATI Journal of Biosciences 17, no. 1 (March 2010): 50–52. http://dx.doi.org/10.4308/hjb.17.1.50.

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41

Nair, S., T. K. Lin, T. Pang, and M. Altwegg. "Characterization of Salmonella Serovars by PCR-Single-Strand Conformation Polymorphism Analysis." Journal of Clinical Microbiology 40, no. 7 (July 1, 2002): 2346–51. http://dx.doi.org/10.1128/jcm.40.7.2346-2351.2002.

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42

Spagnolo, Dominic V., Gavin R. Turbett, Brett Dix, and Barry Iacopetta. "Polymerase Chain Reaction and Single-Strand Conformation Polymorphism Analysis (PCR–SSCP)." Advances in Anatomic Pathology 1, no. 2 (September 1994): 61–77. http://dx.doi.org/10.1097/00125480-199409000-00001.

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43

Gasser, Robin B., Min Hu, Neil B. Chilton, Bronwyn E. Campbell, Aaron J. Jex, Domenico Otranto, Claudia Cafarchia, Ian Beveridge, and Xingquan Zhu. "Single-strand conformation polymorphism (SSCP) for the analysis of genetic variation." Nature Protocols 1, no. 6 (December 2006): 3121–28. http://dx.doi.org/10.1038/nprot.2006.485.

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44

Pinheiro, N. A., R. P. Moura, E. Monteiro, and L. L. Villa. "Detection of point mutations by non-isotopic single strand conformation polymorphism." Brazilian Journal of Medical and Biological Research 32, no. 1 (January 1999): 55–58. http://dx.doi.org/10.1590/s0100-879x1999000100008.

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45

Blasczyk, R., U. Hahn, N. Schwella, and D. Huhn. "PCR-single-strand conformation polymorphism analysis of the HLA-A gene." Human Immunology 39, no. 2 (February 1994): 140. http://dx.doi.org/10.1016/0198-8859(94)90188-0.

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46

Summers, Colin, William Fergusson, David Gokhale, and Malcolm Taylor. "Donor-recipient bone-marrow matching by single strand conformation polymorphism analysis." Lancet 339, no. 8793 (March 1992): 621. http://dx.doi.org/10.1016/0140-6736(92)90911-l.

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47

Kakavas, Konstantinos V. "Sensitivity and applications of the PCR Single-Strand Conformation Polymorphism method." Molecular Biology Reports 48, no. 4 (April 2021): 3629–35. http://dx.doi.org/10.1007/s11033-021-06349-2.

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48

Martins-Lopes, P., H. Zhang, and R. Koebner. "Detection of single nucleotide mutations in wheat using single strand conformation polymorphism gels." Plant Molecular Biology Reporter 19, no. 2 (June 2001): 159–62. http://dx.doi.org/10.1007/bf02772158.

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49

Pieneman, W. C., P. H. Reitsma, and E. Briët. "Double Strand Conformation Polymorphism (DSCP) Detects Two Point Mutations at Codon 280 (AAC→ATC) and at Codon 431 (TAC→AAC) of the Blood Coagulation Factor VIII Gene." Thrombosis and Haemostasis 69, no. 05 (1993): 473–75. http://dx.doi.org/10.1055/s-0038-1651635.

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SummaryHemophilia A is a hereditary, X-linked, bleeding disorder that is caused by a defect in the factor VIII gene. Here, we report two novel point mutations in the factor VIII gene that result in an aberrant electrophoretic mobility of double strand PCR fragments (double strand conformation polymorphism, DSCP). In exon 9 a TAC→AAC mutation at codon 431, replacing Tyr by Asn, was observed in a family (A211) with moderately severe hemophilia A. A family with mild hemophilia A revealed an A→T mutation in codon 280 (exon 7) that results in the replacement of Asn by Ile. One of these two mutations was not detected in an analysis based on single strand conformation polymorphisms (SSCP).At present we have no explanation for the effect of the nucleotide changes on the electrophoretic mobility of double strand DNA. Although DSCP is not able to detect all mutations the combination of DSCP analysis with SSCP analysis increases the sensitivity in a screening for factor VIII mutations.
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BARROSO, ANGEL, SUSANA DUNNER, and JAVIER CAÑON. "Use of a single-strand conformation polymorphism analysis to perform a simple genotyping of bovine κ-casein A and B variants." Journal of Dairy Research 64, no. 4 (November 1997): 535–40. http://dx.doi.org/10.1017/s0022029997002471.

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We propose an alternative method for casein genotyping, generally carried out using polymerase chain reaction followed by restriction fragment length polymorphism analysis. Application of the single-strand conformation polymorphism technique detects nucleotide changes in the fragment amplified by means of polymerase chain reaction and thus avoids the use of restriction enzymes. A 453 bp fragment from exon IV of κ-casein has been amplified. The two variants (A and B), found with the highest frequencies in most bovine breeds and included in some dairy cattle selection schemes, can be discriminated using single-strand conformation polymorphism analysis of heat denatured fragments in acrylamide–bis-acrylamide (100[ratio ]1) gels followed by silver staining. κ-Casein genotyping is therefore simplified, although variants A and E on the one hand, and B and C on the other, are not distinguishable with this technique.
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