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Relevant bibliographies by topics / Single Strand Conformation Polymorphism

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Academic literature on the topic 'Single Strand Conformation Polymorphism'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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Journal articles on the topic "Single Strand Conformation Polymorphism"

1

Maréchal, V. "SSCP (single strand conformation polymorphism)." EMC - Biologie médicale 3, no. 1 (January 2008): 1–5. http://dx.doi.org/10.1016/s2211-9698(08)71401-8.

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Kaczanowski, Radoslaw, Lech Trzeciak, and Krzysztof Kucharczyk. "Multitemperature single-strand conformation polymorphism." ELECTROPHORESIS 22, no. 16 (September 2001): 3539–45. http://dx.doi.org/10.1002/1522-2683(200109)22:16<3539::aid-elps3539>3.0.co;2-t.

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Jedlicka, Anne E., and Steven R. Kleeberger. "Single-Strand Conformation Polymorphism Analysis." Cold Spring Harbor Protocols 2006, no. 1 (June 2006): pdb.prot4118. http://dx.doi.org/10.1101/pdb.prot4118.

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Castellv�-Bel, S., A. S�nchez, C. Badenas, J. Mallolas, A. Barcel�, D. Jim�nez, M. Villa, X. Estivill, and M. Mil�. "Single-strand conformation polymorphism analysis in theFMR1." American Journal of Medical Genetics 84, no. 3 (May 28, 1999): 262–65. http://dx.doi.org/10.1002/(sici)1096-8628(19990528)84:3<262::aid-ajmg18>3.0.co;2-v.

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Fujita, K., and J. Silver. "Single-strand conformational polymorphism." Genome Research 4, no. 3 (December 1, 1994): S137—S140. http://dx.doi.org/10.1101/gr.4.3.s137.

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Krizkova, L., R. Sakthivel, S. A. Olowe, P. K. Rogan, and J. Floros. "Human SP-A: genotype and single-strand conformation polymorphism analysis." American Journal of Physiology-Lung Cellular and Molecular Physiology 266, no. 5 (May 1, 1994): L519—L527. http://dx.doi.org/10.1152/ajplung.1994.266.5.l519.

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We have previously characterized two surfactant protein A (SP-A) cDNAs termed 1A and 6A, as well as a 6A allelic variant termed 6A1. These sequences are quite heterogeneous at the 3' untranslated region (3'UT). Differences between 6A and 6A1 alleles include an 11-bp insertion/deletion 407 bases downstream from the start of the translation termination codon and a base pair polymorphism (C or G) in exon 1 (position 1,193; White, Damm, Miller, Spratt, Schilling, Hawgood, Benson, and Cordell. Nature Lond. 317: 361–363, 1985). The 11-bp (GCCCACTGCCT) segment is present in 6A1 and absent in 6A. The 6A/6A genotype, in a small number of specimens, showed a trend toward a higher frequency in the black Nigerian population compared with Caucasians. In this report, we examine the frequency of the 6A genotype in a larger number of samples from Caucasians and black Nigerians as well as the meiotic stability of the 3'UT heterogeneity. Slot-blot analysis and allele-specific oligonucleotide probes have confirmed that the 6A/6A genotype is more frequent in the Nigerian population. Single-strand conformation polymorphisms in the 3'UT appear to be stably inherited by members of a three-generation family, suggesting that these nucleotide variants represent natural polymorphisms in the population.

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Satoh, T., M. Kobayashi, M. Kaneda, M. Tanihiro, K. Okada, and K. Ueda. "Genotypical classification of neutrophil Fc gamma receptor III by polymerase chain reaction-single-strand conformation polymorphism." Blood 83, no. 11 (June 1, 1994): 3312–15. http://dx.doi.org/10.1182/blood.v83.11.3312.3312.

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Abstract We classified the genotype of neutrophil Fc gamma receptor III (FcRIII) (CD16) with a new method. Genomic DNA from mononuclear cells of 39 unrelated healthy donors (13 NA1/NA1, 13 NA2/NA2, and 13 NA1/NA2 typed serologically) were subjected to polymerase chain reaction (PCR) to amplify the polymorphic third exon of the FcRIII genes. The PCR products were heat denatured, electrophoresed, and visualized by silver staining. Allelic differences were detected by distinctive electrophoretic patterns of each single strand, depending on their sequence specific conformations (single-strand conformation polymorphism [SSCP]). The genotypes of neutrophil FcRIII determined by this method were consistent with the phenotypes of NA antigens determined by serologic examinations. These results indicate that the PCR-SSCP system is a very useful tool for genotyping of the neutrophil FcRIII.

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Satoh, T., M. Kobayashi, M. Kaneda, M. Tanihiro, K. Okada, and K. Ueda. "Genotypical classification of neutrophil Fc gamma receptor III by polymerase chain reaction-single-strand conformation polymorphism." Blood 83, no. 11 (June 1, 1994): 3312–15. http://dx.doi.org/10.1182/blood.v83.11.3312.bloodjournal83113312.

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We classified the genotype of neutrophil Fc gamma receptor III (FcRIII) (CD16) with a new method. Genomic DNA from mononuclear cells of 39 unrelated healthy donors (13 NA1/NA1, 13 NA2/NA2, and 13 NA1/NA2 typed serologically) were subjected to polymerase chain reaction (PCR) to amplify the polymorphic third exon of the FcRIII genes. The PCR products were heat denatured, electrophoresed, and visualized by silver staining. Allelic differences were detected by distinctive electrophoretic patterns of each single strand, depending on their sequence specific conformations (single-strand conformation polymorphism [SSCP]). The genotypes of neutrophil FcRIII determined by this method were consistent with the phenotypes of NA antigens determined by serologic examinations. These results indicate that the PCR-SSCP system is a very useful tool for genotyping of the neutrophil FcRIII.

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Burri, Nathalie, and Pascal Chaubert. "Complex Methylation Patterns Analyzed by Single-Strand Conformation Polymorphism." BioTechniques 26, no. 2 (February 1999): 232–34. http://dx.doi.org/10.2144/99262bm10.

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10

Blanché, Hélène, Christel Valette, and Christine Bellanné-Chantelot. "Optimization of Nonisotopic PCR–Single-Strand Conformation Polymorphism Analysis." Clinical Chemistry 43, no. 11 (November 1, 1997): 2190–92. http://dx.doi.org/10.1093/clinchem/43.11.2190.

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Dissertations / Theses on the topic "Single Strand Conformation Polymorphism"

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Charinthon, Ngamamonpirat Jitra Waikagul. "Sequence variation of Gnathostoma spinigerum mitochondrial DNA by single-strand conformation polymorphism analysis /." Abstract, 2003. http://mulinet3.li.mahidol.ac.th/thesis/2546/46E-Charinthon-N.pdf.

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King, Stephanie. "Capillary Electrophoresis Single-Strand Conformation Polymorphism Analysis for Monitoring Bacteria during the Remediation of TNT-Contaminated Soil." Ohio University / OhioLINK, 2004. http://www.ohiolink.edu/etd/view.cgi?ohiou1108061640.

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ZIHA, ZARIFI ISABELLE. "Mutations de la p53 dans les cancers du sein : optimisation des conditions de single strand conformation polymorphism." Besançon, 1993. http://www.theses.fr/1993BESA3105.

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Genini, Sem. "Establishment of quick-methods to reveal DNA-polymorphisms single strand conformational polymorphism (SSCP) and heteroduplex analysis (HA) /." Zurich : Swiss Federal Institute of Technology, Department of Animal Sciences, Breeding Biology, 2002. http://e-collection.ethbib.ethz.ch/show?type=dipl&nr=50.

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Jorge, Simone Bordignon de. "Otimização da tecnica de SSCP (Single Strand Conformation Polymorphism) para triagem de mutações nos genes da globina [alfa] humana." [s.n.], 2002. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310884.

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Orientadores : Maria de Fatima Sonati, Monica Barbosa de Melo
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
Made available in DSpace on 2018-08-03T14:43:34Z (GMT). No. of bitstreams: 1 Jorge_SimoneBordignonde_M.pdf: 1672707 bytes, checksum: 1ca1b039159c022f6091792558490b00 (MD5) Previous issue date: 2002
Resumo: Mutações de ponto e pequenas inserções ou deleções nos genes da globina a humana podem produzir variantes estruturais de cadeia a e a-talassemia. As mutações podem ser identificadas tanto pelo sequenciamento total dos genes, como por métodos de triagem, os quais selecionam o exon mutado para, então, ser seqüenciado. Embora sejam pequenos (cerca de 1Kb, com 3 exons e 2 introns), os genes da globina a são duplicados (a2 and a1) e extremamente ricos em ligações G-C, o que torna difícil a desnaturação e reduz a eficiência do sequenciamento, causando freqüentes artefatos e necessidade de repetições. Como o sequenciamento é ainda um método demorado e de custo elevado para muitos laboratórios, nós modificamos algumas condições do Single Strand Conformation Polymorphism (SSCP) com o intuito de otimizar a triagem de mutações nos genes da globina a humana
Abstract: Point mutations and small insertions or deletions in the human a-globin genes may produce a-chain structural variants and a-thalassemia. Mutations can be detected either by direct sequencing of the whole gene or by screening methods, which select the mutated exon for sequencing. Although small (around 1 Kb, 3 exons and 2 introns), the a-globin genes are duplicate (a2 and a1) and extremely G-C rich, what makes them difficult to denature and reduces sequencing efficiency, causing frequent artifacts and the need of repetitions. As DNA sequencing is still a time-consuming and expensive method for many laboratories, we modified some conditions of the Single Strand Conformation Polymorphism (SSCP) in order to optimize mutation detection in the a-globin genes
Mestrado
Ciencias Biomedicas
Mestre em Ciências Médicas

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Contador, Luciana. "Etude de la structure chimique et microbiologique de l'interface air-mer en baie de Guanabara (Rio de Janeiro, Brésil)." Paris 6, 2006. http://www.theses.fr/2006PA066158.

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La microcouche de surface (SML) correspond au premier millimètre de la colonne d’eau. Située à l’interface air-eau, la SML est un environnement unique, riche en microorganismes, en matières organiques, en hydrocarbures et présentant des conditions extrêmes par l’impact des radiations UV et les fortes concentrations de polluants. Les échantillons ont été prélevés dans la SML et dans l’eau sous-jacente (UW) à 7 stations de la Baie de Guanabara, Brésil. Cette étude a permis de caractériser l’abondance et la diversité des bactéries et des hydrocarbures présents dans la SML de la baie. L’origine des hydrocarbures a été analysée par des marqueurs chimiques par chromatographie en phase gazeuse. Sur le plan microbiologique, 43 souches bactériennes ont été isolées et identifiées. Leur résistance aux radiations UV a été évaluée à l’aide d’un simulateur solaire. La SML de la baie est bien définie par rapport à l’UW et enrichie en hydrocarbures et en bactéries. Le bactérioneuston est plus diversifié que le bactérioplancton. La plupart des souches testées sont très résistantes aux UV et sont des espèces potentiellement intéressantes sur le plan biotechnologique.

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Duthoit, Frédérique. "Dynamique des communautés microbiennes du fromage d'A. O. C. Salers par analyse Single-Strand Conformation Polymorphism : lien avec les caractéristiques sensorielles des fromages." Clermont-Ferrand 2, 2003. http://www.theses.fr/2003CLF21453.

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Le but de ce travail est de relier les dynamiques microbiennes de présence et d'activité suivies par analyse Single Strand Conformation Polymorphism au cours de la fabrication du fromage d'AOC Salers aux caractéristiques sensorielles du fromage affiné. Des régions des ADNr et ARNr 16S extraits des fromages ont été amplifiées par PCR et RT-PCR et analysées par SSCP. Les profils microbiens ont été reliés aux données sensorielles par régression Partial Least Square. Des séquences correspondant à des bactéries lactiques, entérobactéries, bactéries Gram positif à bas et haut GC% ont été identifiées. La comparaison des profils issus des ARN et des ADN montre des dynamiques bactériennes et des niveaux d'activité différents. Les corrélations avec les variables sensorielles sont complexes. Chaque descripteur est expliqué par de nombreux groupes microbiens. Cela montre l'intérêt de prendre en compte les dynamiques bactériennes de présence et d'activité lors de la fabrication des fromages

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Hedley, Paula. "The development of the single-strand conformation polymorphism (SSCP) technique to assess sequence level variation within the major histocompatibility complex (MHC) DRB1 gene in four South African buffalo (Syncerus caffer) populations." Master's thesis, University of Cape Town, 2003. http://hdl.handle.net/11427/4271.

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Includes bibliographical references.
This thesis reports the development of Single-Strand Conformation Polymorphism (SSCP) technique to assess sequence level variation within the Major Histocompatibility Complex (MHC) DRB1 gene in four South African buffalo populations. MHC gene products are involved in the immune response, and so variation within these genes provides information on the immunological fitness of the population under study. The aims of this study were: (i) to develop the SSCP technique; (ii) to investigate the level of genetic variation at the peptide binding region (PBR) of the DRB1 gene in four South African buffalo populations. (iii) This data was then compared to data generated previously in a study on the same populations using microsatellite DNA, (iv) the statistical comparisons were used to assess the appropriateness of SSCP data for population genetic analysis. Levels of heterozygosity, allelic diversity and population differentiation were quantified using MHC DRB1 gene. The amplified region (Exon 2 of the DRB1 gene) showed high levels of variability, with 77 alleles found in the 84 individuals examined using SSCP analyses.

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9

Peyruchaud, Olivier. "Analyse des polymorphismes du complexe glycoprotéique GPIIb-IIIa plaquettaire par PCR-SSCP : application à la Thrombasthénie de Glanzmann et aux systèmes alloantigéniques." Bordeaux 2, 1995. http://www.theses.fr/1995BOR28356.

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Contador, Luciana. "Étude de la structure chimique et microbiologique de l'interface air-mer en Baie de Guanabara (Rio de Janeiro, Brésil)." Phd thesis, Université Pierre et Marie Curie - Paris VI, 2006. http://tel.archives-ouvertes.fr/tel-00122547.

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La microcouche de surface (SML) correspond au premier millimètre de la colonne d'eau. Située à l'interface air-eau, la SML est un environnement unique, riche en microorganismes, en matières organiques, en hydrocarbures et présentant des conditions extrêmes par l'impact des radiations UV et les fortes concentrations de polluants. Les échantillons ont été prélevés dans la SML et dans l'eau sous-jacente (UW) à 7 stations de la Baie de Guanabara, Brésil. Cette étude a permis de caractériser l'abondance et la diversité des bactéries et des hydrocarbures présents dans la SML de la baie. L'origine des hydrocarbures a été analysée par des marqueurs chimiques par chromatographie en phase gazeuse. Sur le plan microbiologique, 43 souches bactériennes ont été isolées et identifiées. Leur résistance aux radiations UV a été évaluée à l'aide d'un simulateur solaire. La SML de la baie est bien définie par rapport à l'UW et enrichie en hydrocarbures et en bactéries. Le bactérioneuston est plus diversifié que le bactérioplancton. La plupart des souches testées sont très résistantes aux UV et sont des espèces potentiellement intéressantes sur le plan biotechnologique.

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Book chapters on the topic "Single Strand Conformation Polymorphism"

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Sekiya, Takao. "Single-Strand Conformation Polymorphism Analysis." In Technologies for Detection of DNA Damage and Mutations, 281–90. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-0301-3_21.

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Leung, Kim Hung, and Shea Ping Yip. "Single Strand Conformation Polymorphism (SSCP) Analysis." In Springer Protocols Handbooks, 117–31. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-375-6_9.

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Jordan, William C., Katherine Foley, and Michael W. Bruford. "Single-Strand Conformation Polymorphism (SSCP) Analysis." In Molecular Tools for Screening Biodiversity, 152–56. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-009-0019-6_30.

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Qian, Feng, and Gregory G. Germino. "Single-Strand Conformation Polymorphism (SSCP) Analysis." In Techniques in Molecular Medicine, 82–85. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59811-1_5.

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Vorechovsky, Igor. "Single-Strand Conformation Polymorphism (SSCP) Analysis." In Medical Biomethods Handbook, 73–77. Totowa, NJ: Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-870-6:073.

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Musić, Martina Šeruga, and Dijana Škorić. "Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains." In Methods in Molecular Biology, 217–22. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-089-2_18.

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Schmalenberger, Achim, and Christoph C. Tebbe. "Profiling the Diversity of Microbial Communities with Single-Strand Conformation Polymorphism (SSCP)." In Methods in Molecular Biology, 71–83. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-712-9_6.

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Lum, Michelle R., and Ann M. Hirsch. "Detecting the Components of Botanical Mixtures by Single-Strand Conformation Polymorphism Analysis." In ACS Symposium Series, 351–62. Washington, DC: American Chemical Society, 2011. http://dx.doi.org/10.1021/bk-2011-1081.ch024.

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Offermans, M. T. C., H. Meyer, and N. D. Zegers. "Identification of Pathogens Using Single/Double Strand Conformation Polymorphism (SSCP/DSCP) Analysis." In Rapid Methods for Analysis of Biological Materials in the Environment, 251–58. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-015-9534-6_20.

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Lin, Shu-Wha, Shu-Rung Lin, and Ming-Ching Shen. "Hemophilia A: Characterization of Genetic Defects by the Single Strand Conformation Polymorphism (SSCP)." In Current Aspects of Blood Coagulation, Fibrinolysis, and Platelets, 55–57. Tokyo: Springer Japan, 1993. http://dx.doi.org/10.1007/978-4-431-68323-0_9.

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Conference papers on the topic "Single Strand Conformation Polymorphism"

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Zhao, Yan, Jianzheng Li, Jie Zhang, Dong Li, and Shuang Zhao. "Dynamics of Methangens During Steady-State of Anaerobic Baffled Reactor Using Single Strand Conformation Polymorphism." In 2009 Asia-Pacific Power and Energy Engineering Conference. IEEE, 2009. http://dx.doi.org/10.1109/appeec.2009.4918306.

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Ono, Koichi, Mitsuyasu Koike, Takuhito Ohse, Atsuko Takagi, and Yasuyuki Ikeda. "High-throughput Single-strand Conformation Polymorphism Analysis of an LPL Gene Mutation by Temperature-controlled On-chip Capillary Electrophoresis." In 2006 International Conference on Microtechnologies in Medicine and Biology. IEEE, 2006. http://dx.doi.org/10.1109/mmb.2006.251527.

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Maghsoodi, Ameneh, Anupam Chatterjee, Ioan Andricioaei, and Noel Perkins. "An Approximate Model of the Dynamics of the Bacteriophage T4 Injection Machinery." In ASME 2016 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/detc2016-60281.

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Bacteriophage T4 is one of the most common and complex of the tailed viruses that infect host bacteria using an intriguing contractile tail assembly. Despite extensive progress in resolving the structure of T4, the dynamics of the injection machinery remains largely unknown. This paper contributes a first model of the injection machinery that is driven by elastic energy stored in a structure known as the sheath. The sheath is composed of helical strands of protein that suddenly collapse from an energetic, extended conformation prior to infection to a relaxed, contracted conformation during infection. We employ Kirchhoff rod theory to simulate the nonlinear dynamics of a single protein strand coupled to a model for the remainder of the virus, including the coupled translation and rotation of the head (capsid), neck and tail tube. Doing so provides an important building block towards the future goal of modeling the entire sheath structure which is composed of six interacting helical protein strands. The resulting numerical model exposes fundamental features of the injection machinery including the time scale and energetics of the infection process, the nonlinear conformational change experienced by the sheath, and the contribution of hydrodynamic drag on the head (capsid).

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Chang, Tsung-Yao, and Chii-Wann Lin. "Solutions of SAT Problems Solved by a SPR-Based DNA Processor." In ASME 2008 First International Conference on Micro/Nanoscale Heat Transfer. ASMEDC, 2008. http://dx.doi.org/10.1115/mnht2008-52036.

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DNA computation has shown its potential not only on mathematics, but on various practical areas such as diagnosis, single nucleotide polymorphism (SNP) detection, smart amplification, encryption and drug delivery etc. However, most previous researches about DNA computation possess a couple of weaknesses, including time-wasting, effort-wasting, lack of reusability and no miniaturization. In order to address this issue and improve weaknesses mentioned above, we built up a surface plasmon resonance (SPR) system for the detection on the DNA array chip to solve a 3-clause satisfiability (SAT) problem with 3 variables x, y and z. Every variable is defined as a 15-nt single strand DNA (ssDNA) which is immobilized on one spot of the gold surface. We further define SPR reflective intensity changes 0, 0.2 and 2 A.U. caused by changes of molecular weight on surface as Boolean signals False, True and None, respectively. Moreover, False signal represents a positive hybridization reaction via the hydrogen bond that binds the complementary ssDNA conjugating to IgG (150 kDa) by the crosslinker Sulfo-SMPB which can links thiol group labeled on ssDNA and amine group existed on IgG; True signal represents the hybridization reaction that binds the complementary ssDNA-IgG-Antigen which can result in a much larger intensity change. For a SAT problem F = (XT ∪ YF)∩(XF ∪ ZT)∩(YT ∪ ZT), there are 8 possible answers. Therefore, we established a 3-spot array as a set which is immobilized sequence x, y and z. After one calculation, we read out the solution of this set and then regenerate it by injecting 0.05 N sodium hydroxide solution. In this work, we used 200 ng/ml Xc-Human IgG, Yc-Rabbit IgG and Zc-Goat IgG as reaction agents for hybridization which represent False signal. Furthermore, 100 ng/ml Anti-Human IgG, Rabbit IgG and Goat IgG were taken as True signal agents. A full reaction can be completed within 30 minutes at room temperature. The buffer in the study is 1 x phosphate buffered solution (PBS) with 100 mM sodium chloride. The proposed platform has improved drawbacks that occurred in previous researches about DNA computer. Besides, it is provided with abilities a processor should have which are recalculable and realtime measurement so that provides us a novel approach for addressable and reusable diagnosis.

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