Relevant bibliographies by topics / Single-ion channel

Academic literature on the topic 'Single-ion channel'

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Published: 4 June 2021
Last updated: 29 January 2023

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Journal articles on the topic "Single-ion channel"

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Plested, Andrew J. R. "Single-Channel Recording of Ligand-Gated Ion Channels." Cold Spring Harbor Protocols 2016, no. 8 (August 2016): pdb.top087239. http://dx.doi.org/10.1101/pdb.top087239.

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Mortensen, Martin, and Trevor G. Smart. "Single-channel recording of ligand-gated ion channels." Nature Protocols 2, no. 11 (November 2007): 2826–41. http://dx.doi.org/10.1038/nprot.2007.403.

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JANG, J. H., S. D. KIM, J. B. PARK, S. J. HONG, and P. D. RYU. "Ion channels ofFasciola hepaticaincorporated into planar lipid bilayers." Parasitology 128, no. 1 (January 2004): 83–89. http://dx.doi.org/10.1017/s0031182003004232.

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Ion channels are important target sites of anthelmintics, but little is known about those inFasciola hepatica. In this work, we applied a planar lipid bilayer technique to characterize the properties of single ion channels inF. hepatica. Under a 200/40 mMKCl gradient, a large conductance channel of 251 pS was observed in 18% of the membranes studied. The channel was selective to K+over Cl−with a permeability ratio of K+to Cl−(PK/PCl) of 4·9. Open state probability (Po) of the channel was less than 0·5 and dependent on voltage (−60~+40 mV) and Ca2+(~100 μM). The other two types of single channels observed in 11 and 5% of membranes, respectively, were a K+-permeable channel of 80 pS (PK/PCl=4·6) and a Cl−-permeable channel of 64 pS (PK/PCl=0·058). Open state probability of both channels showed little voltage dependence. The results indicate that distinct single channels of 60~251 pS are present in relative abundance and, in addition, that the planar lipid bilayer technique can be a useful tool for the study of single ion channels inF. hepatica.
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Baker, Mariah R., Guizhen Fan, and Irina I. Serysheva. "Single-particle cryo-EM of the ryanodine receptor channel." European Journal of Translational Myology 25, no. 1 (January 12, 2015): 35. http://dx.doi.org/10.4081/ejtm.2015.4803.

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Ryanodine receptors (RyRs) are tetrameric ligand-gated Ca2+ release channels that are responsible for the increase of cytosolic Ca2+ concentration leading to muscle contraction. Our current understanding of RyR channel gating and regulation is greatly limited due to the lack of a high-resolution structure of the channel protein. The enormous size and unwieldy shape of Ca2+ release channels make X-ray or NMR methods difficult to apply for high-resolution structural analysis of the full-length functional channel. Single-particle electron cryo-microscopy (cryo-EM) is one of the only effective techniques for the study of such a large integral membrane protein and its molecular interactions. Despite recent developments in cryo-EM technologies and break-through single-particle cryo-EM studies of ion channels, cryospecimen preparation, particularly the presence of detergent in the buffer, remains the main impediment to obtaining atomic-resolution structures of ion channels and a multitude of other integral membrane protein complexes. In this review we will discuss properties of several detergents that have been successfully utilized in cryo-EM studies of ion channels and the emergence of the detergent alternative amphipol to stabilize ion channels for structure-function characterization. Future structural studies of challenging specimen like ion channels are likely to be facilitated by cryo-EM amenable detergents or alternative surfactants.
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Fox, J. A., B. A. Pfeffer, and G. L. Fain. "Single-channel recordings from cultured human retinal pigment epithelial cells." Journal of General Physiology 91, no. 2 (February 1, 1988): 193–222. http://dx.doi.org/10.1085/jgp.91.2.193.

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We have applied patch-clamp techniques to on-cell and excised-membrane patches from human retinal pigment epithelial cells in tissue culture. Single-channel currents from at least four ion channel types were observed: three or more potassium-selective channels with single-channel slope conductances near 100, 45, and 25 pS as measured in on-cell patches with physiological saline in the pipette, and a relatively nonselective channel with subconductance states, which has a main-state conductance of approximately 300 pS at physiological ion concentrations. The permeability ratios, PK/PNa, measured in excised patches were 21 for the 100-pS channels, 3 for the 25-pS channels, and 0.8 for the 300-pS nonselective channel. The 45-pS channels appeared to be of at least two types, with PK/PNa's of approximately 41 for one type and 3 for the other. The potassium-selective channels were spontaneously active at all potentials examined. The average open time for these channels ranged from a few milliseconds to many tens of milliseconds. No consistent trend relating potassium-selective channel kinetics to membrane potential was apparent, which suggests that channel activity was not regulated by the membrane potential. In contrast to the potassium-selective channels, the activity of the nonselective channel was voltage dependent: the open probability of this channel declined to low values at large positive or negative membrane potentials and was maximal near zero. Single-channel conductances observed at several symmetrical KCl concentrations have been fitted with Michaelis-Menten curves in order to estimate maximum channel conductances and ion-binding constants for the different channel types. The channels we have recorded are probably responsible for the previously observed potassium permeability of the retinal pigment epithelium apical membrane.
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Rodriguez-Contreras, Adrian, Ping Lv, Jun Zhu, Hyo Jeong Kim, and Ebenezer N. Yamoah. "Effects of Strontium on the Permeation and Gating Phenotype of Calcium Channels in Hair Cells." Journal of Neurophysiology 100, no. 4 (October 2008): 2115–24. http://dx.doi.org/10.1152/jn.90473.2008.

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To minimize the effects of Ca2+ buffering and signaling, this study sought to examine single Ca2+ channel properties using Sr2+ ions, which substitute well for Ca2+ but bind weakly to intracellular Ca2+ buffers. Two single-channel fluctuations were distinguished by their sensitivity to dihydropyridine agonist (L-type) and insensitivity toward dihydropyridine antagonist (non-L-type). The L- and non-L-type single channels were observed with single-channel conductances of 16 and 19 pS at 70 mM Sr2+ and 11 and 13 pS at 5 mM Sr2+, respectively. We obtained KD estimates of 5.2 and 1.9 mM for Sr2+ for L- and non-L-type channels, respectively. At Ca2+ concentration of ∼2 mM, the single-channel conductances of Sr2+ for the L-type channel was ∼1.5 and 4.0 pS for the non-L-type channels. Thus the limits of single-channel microdomain at the membrane potential of a hair cell (e.g., −65 mV) for Sr2+ ranges from 800 to 2,000 ion/ms, assuming an ECa of 100 mV. The channels are ≥4-fold more sensitive at the physiological concentration ranges than at concentrations >10 mM. Additionally, the channels have the propensity to dwell in the closed state at high concentrations of Sr2+, which is reflected in the time constant of the first latency distributions. It is concluded that the concentration of the permeant ion modulates the gating of hair cell Ca2+ channels. Finally, the closed state/s that is/are altered by high concentrations of Sr2+ may represent divalent ion-dependent inactivation of the L-type channel.
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Oh, Y., and D. J. Benos. "Single-channel characteristics of a purified bovine renal amiloride-sensitive Na+ channel in planar lipid bilayers." American Journal of Physiology-Cell Physiology 264, no. 6 (June 1, 1993): C1489—C1499. http://dx.doi.org/10.1152/ajpcell.1993.264.6.c1489.

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We have purified an amiloride-inhibitable Na+ channel protein from bovine renal papillae using ion-exchange and immunoaffinity chromatography. In the present study, these purified Na+ channels were reconstituted into planar lipid bilayers, and their single-channel characteristics were studied. We observed both large- and small-conductance Na(+)-selective ion channels in planar lipid bilayers. Single-channel conductance for the large- and small-conductance channels saturated as a function of Na+ concentration. These relations could be fitted by a simple Langmuir isotherm with a Michaelis constant of 55 and 45 mM and a maximum open-state conductance of 56 or 8.4 pS, respectively. Both channels were perfectly cation selective, with a Na(+)-to-K+ permeability ratio of 6.7:1 for the large channel and 7.8:1 for the small channel, and their open single-channel current-voltage relations were linear when bathed with symmetrical Na+ solutions. The percent open time of the reconstituted large or small channels varied between 10 and 50% or 1 and 20%, respectively. After application of amiloride, both the large- and small-conductance Na+ channels were inhibited in a dose-dependent manner.
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Lozanović Šajić, Jasmina, Sonja Langthaler, and Christian Baumgartner. "Creating a Novel Mathematical Model of the Kv10.1 Ion Channel and Controlling Channel Activity with Nanoelectromechanical Systems." Applied Sciences 12, no. 8 (April 11, 2022): 3836. http://dx.doi.org/10.3390/app12083836.

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The use of nanoelectromechanical systems or nanorobots offers a new concept for sensing and controlling subcellular structures, such as ion channels. We present here a novel method for mathematical modeling of ion channels based on control system theory and system identification. We investigated the use of nanoelectromechanical devices to control the activity of ion channels, particularly the activity of the voltage-gated ion channel Kv10.1, an important channel in cancer development and progression. A mathematical model of the dynamic behavior of the selected ion channel Kv10.1 in the Laplace (s) domain was developed, which is given in the representation of a transfer function. In addition, we addressed the possibilities of controlling ion channel activity by nanoelectromechanical devices and nanorobots and finally presented a control algorithm for the Kv10.1 as a control object. A use case demonstrates the potential of a Kv10.1 controlled nanorobot for cancer treatment at a single-cell level.
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Gao, Jianzhao, Hong Wei, Alberto Cano, and Lukasz Kurgan. "PSIONplusm Server for Accurate Multi-Label Prediction of Ion Channels and Their Types." Biomolecules 10, no. 6 (June 7, 2020): 876. http://dx.doi.org/10.3390/biom10060876.

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Computational prediction of ion channels facilitates the identification of putative ion channels from protein sequences. Several predictors of ion channels and their types were developed in the last quindecennial. While they offer reasonably accurate predictions, they also suffer a few shortcomings including lack of availability, parallel prediction mode, single-label prediction (inability to predict multiple channel subtypes), and incomplete scope (inability to predict subtypes of the voltage-gated channels). We developed a first-of-its-kind PSIONplusm method that performs sequential multi-label prediction of ion channels and their subtypes for both voltage-gated and ligand-gated channels. PSIONplusm sequentially combines the outputs produced by three support vector machine-based models from the PSIONplus predictor and is available as a webserver. Empirical tests show that PSIONplusm outperforms current methods for the multi-label prediction of the ion channel subtypes. This includes the existing single-label methods that are available to the users, a naïve multi-label predictor that combines results produced by multiple single-label methods, and methods that make predictions based on sequence alignment and domain annotations. We also found that the current methods (including PSIONplusm) fail to accurately predict a few of the least frequently occurring ion channel subtypes. Thus, new predictors should be developed when a larger quantity of annotated ion channels will be available to train predictive models.
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Lu, Tao, Li Wu, Jun Xiao, and Jian Yang. "Permeant Ion-Dependent Changes in Gating of Kir2.1 Inward Rectifier Potassium Channels." Journal of General Physiology 118, no. 5 (November 1, 2001): 509–22. http://dx.doi.org/10.1085/jgp.118.5.509.

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We studied the effect of monovalent thallium ion (Tl+) on the gating of single Kir2.1 channels, which open and close spontaneously at a constant membrane potential. In cell-attached recordings of single-channel inward current, changing the external permeant ion from K+ to Tl+ decreases the mean open-time by ∼20-fold. Furthermore, the channel resides predominantly at a subconductance level, which results from a slow decay (τ = 2.7 ms at −100 mV) from the fully open level immediately following channel opening. Mutation of a pore-lining cysteine (C169) to valine abolishes the slow decay and subconductance level, and single-channel recordings from channels formed by tandem tetramers containing one to three C169V mutant subunits indicate that Tl+ must interact with at least three C169 residues to induce these effects. However, the C169V mutation does not alter the single-channel closing kinetics of Tl+ current. These results suggest that Tl+ ions change the conformation of the ion conduction pathway during permeation and alter gating by two distinct mechanisms. First, they interact with the thiolate groups of C169 lining the cavity to induce conformational changes of the ion passageway, and thereby produce a slow decay of single-channel current and a dominant subconductance state. Second, they interact more strongly than K+ with the main chain carbonyl oxygens lining the selectivity filter to destabilize the open state of the channel and, thus, alter the open/close kinetics of gating. In addition to altering gating, Tl+ greatly diminishes Ba2+ block. The unblocking rate of Ba2+ is increased by >22-fold when the external permeant ion is switched from K+ to Tl+ regardless of the direction of Ba2+ exit. This effect cannot be explained solely by ion–ion interactions, but is consistent with the notion that Tl+ induces conformational changes in the selectivity filter.
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Dissertations / Theses on the topic "Single-ion channel"

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Selepova, Pavla. "Single ion channel dynamics." Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=65415.

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Kubota, Shintaro. "Single Channel Analysis of Ion Transport across Membranes Containing Gramicidin A and KAT1 Channels." Kyoto University, 2016. http://hdl.handle.net/2433/215593.

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Kyoto University (京都大学)
0048
新制・課程博士
博士(農学)
甲第19767号
農博第2163号
新制||農||1040(附属図書館)
学位論文||H28||N4983(農学部図書室)
32803
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 加納 健司, 教授 三芳 秀人, 教授 三上 文三
学位規則第4条第1項該当
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Livesey, Matthew Robert. "Molecular determinants of single channel conductance and ion selectivity in cationic Cys-loop receptor channels." Thesis, University of Dundee, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.510623.

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Rosales, Rafael Andres. "Bayesian analysis of hidden Markov models for single ion channel records." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621590.

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Hoyles, Matthew, and Matthew Hoyles@anu edu au. "Computer Simulation of Biological Ion Channels." The Australian National University. Theoretical Physics, 2000. http://thesis.anu.edu.au./public/adt-ANU20010702.135814.

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This thesis describes a project in which algorithms are developed for the rapid and accurate solution of Poisson's equation in the presence of a dielectric boundary and multiple point charges. These algorithms are then used to perform Brownian dynamics simulations on realistic models of biological ion channels. An iterative method of solution, in which the dielectric boundary is tiled with variable sized surface charge sectors, provides the flexibility to deal with arbitrarily shaped boundaries, but is too slow to perform Brownian dynamics. An analytical solution is derived, which is faster and more accurate, but only works for a toroidal boundary. Finally, a method is developed of pre-calculating solutions to Poisson's equation and storing them in tables. The solution for a particular configuration of ions in the channel can then be assembled by interpolation from the tables and application of the principle of superposition. This algorithm combines the flexibility of the iterative method with greater speed even than the analytical method, and is fast enough that channel conductance can be predicted. The results of simulations for a model single-ion channel, based on the acetylcholine receptor channel, show that the narrow pore through the low dielectric strength medium of the protein creates an energy barrier which restricts the permeation of ions. They further show that this barrier can be removed by dipoles in the neck of the channel, but that the barrier is not removed by shielding by counter-ions. The results of simulations for a model multi-ion channel, based on a bacterial potassium channel, show that the model channel has conductance characteristics similar to those of real potassium channels. Ions appear to move through the model multi-ion channel via rapid transitions between a series of semi-stable states. This observation suggests a possible physical basis for the reaction rate theory of channel conductance, and opens up an avenue for future research.
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Orr, Galya. "Modulation of synaptic plasticity by theta rhythm and structure-function relationships in a single ion channel." Diss., The University of Arizona, 2002. http://hdl.handle.net/10150/280098.

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A few studies support the idea that the theta rhythm modulates synaptic plasticity by demonstrating that the phase of the theta cycle at which the induction stimuli are delivered determines the nature of the resulting synaptic plasticity. These studies were conducted in urethane-anesthetized animals and in vitro slice preparations where the theta rhythm is artificially generated. Our goal was to find whether and how natural theta activity affects synaptic plasticity in the hippocampus of adult and old freely behaving animals. In both adult and aged, memory-impaired rats, LTP lasting at least 48 h was induced when stimuli were delivered at theta peak. No change in synaptic strength was observed when stimuli were delivered at theta trough. These observations indicate that the naturally occurring theta rhythm modulates synaptic plasticity, and suggest a mechanism by which the phase of firing could contain meaningful information. The degree of LTP, however, was significantly smaller in the old animals. To better understand the conformational changes and the dynamic interactions that govern ion-channel kinetics we developed a new approach using simultaneous single-channel patch-clamp recording and single-molecule fluorescence microscopy. Gramicidin monomers were tagged with a fluorescence dye and single-channel current was recorded from gramicidin channels in the bilayer that was formed at the tip of a patch pipette. Co-localization and fluorescence resonance energy transfer (FRET) within a single gramicidin dimer were probed. The new technique made it possible to directly capture the conformational dynamics between the two gramicidin monomers by observing the changes in the distance between the attached dye molecules. The molecular interactions of the NMDA receptor with its ligands determine the dynamic properties of activation and desensitization that in turn shape NMDA receptor mediated currents. We have monitored the occurrence and intensity changes of FRET between two fluorescence-labeled agonists at the glutamate binding site of the receptor, simultaneously with single channel current recording. These observations can be translated to dissociation/association rates and aid in our understanding of the mechanisms that underlie the transitions of the receptor between different kinetic states.
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Boassa, Daniela, and Andrea Yool. "Single amino acids in the carboxyl terminal domain of aquaporin-1 contribute to cGMP-dependent ion channel activation." BioMed Central, 2003. http://hdl.handle.net/10150/610075.

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BACKGROUND:Aquaporin-1 (AQP1) functions as an osmotic water channel and a gated cation channel. Activation of the AQP1 ion conductance by intracellular cGMP was hypothesized to involve the carboxyl (C-) terminus, based on amino acid sequence alignments with cyclic-nucleotide-gated channels and cGMP-selective phosphodiesterases.RESULTS:Voltage clamp analyses of human AQP1 channels expressed in Xenopus oocytes demonstrated that the nitric oxide donor, sodium nitroprusside (SNP
3-14 mM) activated the ionic conductance response in a dose-dependent manner. Block of soluble guanylate cyclase prevented the response. Enzyme immunoassays confirmed a linear dose-dependent relationship between SNP and the resulting intracellular cGMP levels (up to 1700 fmol cGMP /oocyte at 14 mM SNP). Results here are the first to show that the efficacy of ion channel activation is decreased by mutations of AQP1 at conserved residues in the C-terminal domain (aspartate D237 and lysine K243).CONCLUSIONS:These data support the idea that the limited amino acid sequence similarities found between three diverse classes of cGMP-binding proteins are significant to the function of AQP1 as a cGMP-gated ion channel, and provide direct evidence for the involvement of the AQP1 C-terminal domain in cGMP-mediated ion channel activation.
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Thei, Federico <1976&gt. "A hybrid technology for parallel recording of single ion channels." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2011. http://amsdottorato.unibo.it/3594/.

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Hybrid technologies, thanks to the convergence of integrated microelectronic devices and new class of microfluidic structures could open new perspectives to the way how nanoscale events are discovered, monitored and controlled. The key point of this thesis is to evaluate the impact of such an approach into applications of ion-channel High Throughput Screening (HTS)platforms. This approach offers promising opportunities for the development of new classes of sensitive, reliable and cheap sensors. There are numerous advantages of embedding microelectronic readout structures strictly coupled to sensing elements. On the one hand the signal-to-noise-ratio is increased as a result of scaling. On the other, the readout miniaturization allows organization of sensors into arrays, increasing the capability of the platform in terms of number of acquired data, as required in the HTS approach, to improve sensing accuracy and reliabiity. However, accurate interface design is required to establish efficient communication between ionic-based and electronic-based signals. The work made in this thesis will show a first example of a complete parallel readout system with single ion channel resolution, using a compact and scalable hybrid architecture suitable to be interfaced to large array of sensors, ensuring simultaneous signal recording and smart control of the signal-to-noise ratio and bandwidth trade off. More specifically, an array of microfluidic polymer structures, hosting artificial lipid bilayers blocks where single ion channel pores are embededed, is coupled with an array of ultra-low noise current amplifiers for signal amplification and data processing. As demonstrating working example, the platform was used to acquire ultra small currents derived by single non-covalent molecular binding between alpha-hemolysin pores and beta-cyclodextrin molecules in artificial lipid membranes.
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Scheppach, Christian Othmar. "Properties of single calcium-permeable ion channels in neocortical neurons." Thesis, University of Cambridge, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708942.

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Sunderman, Elizabeth R. "Single-channel kinetic analysis of the allosteric transition of rod cyclic nucleotide-gated channels /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/10526.

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Books on the topic "Single-ion channel"

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Sakmann, Bert. Single-Channel Recording. Boston, MA: Springer Science+Business Media, LLC, 2009.

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1942-, Sakmann Bert, and Neher Erwin 1944-, eds. Single-channel recording. 2nd ed. New York: Plenum Press, 1995.

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Single-Channel Recording. 2nd ed. Springer, 2007.

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Thalamocortical Assemblies: How Ion Channels, Single Neurons and Large-Scale Networks Organize Sleep Oscillations. Oxford University Press, USA, 2001.

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Jef ferys, John G. R. Cortical activity: single cell, cell assemblages, and networks. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199688395.003.0004.

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This chapter describes how the activity of neurons produces electrical potentials that can be recorded at the levels of single cells, small groups of neurons, and larger neuronal networks. It outlines how the movement of ions across neuronal membranes produces action potentials and synaptic potentials. It considers how the spatial arrangement of specific ion channels on the neuronal surface can produce potentials that can be recorded from the extracellular space. Finally, it outlines how the layered cellular structure of the neocortex can result in summation of signals from many neurons to be large enough to record through the scalp as evoked potentials or the electroencephalogram.
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Levitan, Irwin B., and Leonard K. Kaczmarek. The Neuron. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199773893.001.0001.

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The Fourth Edition of The Neuron provides a comprehensive first course in the cell and molecular biology of nerve cells. It begins with properties of the many newly discovered ion channels that have emerged through mapping of the genome and which shape the way a single neuron generates varied patterns of electrical activity. It also covers the molecular mechanisms that convert electrical activity into the secretion of neurotransmitter hormones at synaptic junctions between neurons. It discusses the biochemical pathways that are linked to the action of neurotransmitters and that can alter the cellular properties of neurons or sensory cells that transduce information from the outside world into the electrical code used by neurons, and the rapidly expanding knowledge of the molecular factors that induce an undifferentiated cell to become a neuron, and then guide it to form appropriate synaptic connections with its partners. Also addressed is the role of ongoing experience and activity in shaping these connections, and the mechanisms thought to underlie the phenomena of learning and memory.
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Eyre, Janet. Neurodevelopmental disorders. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198569381.003.0189.

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Remarkable advances in the neurosciences, particularly in the fields of genetics, molecular biology, metabolism, and nutrition, have greatly advanced our understanding of how the brain develops and responds to environmental influences. Neurodevelopmental disorders arise from perturbation of these normal developmental processes, by insults from heterogeneous aetiological factors. These factors trigger a sequence of molecular, biochemical, and morphological alterations of the brain, resulting in a morphologically and/ or functionally abnormal brain. Rapidly advancing understanding of basic neurodevelopmental processes has direct relevance to understanding human neurodevelopmental disorders, providing insights into pathogenic mechanisms and revealing new pathways that can be exploited in diagnosis and treatment. Conversely the identification of the molecular bases of several neurodevelopmental disorders has also provided invaluable insights into the mechanisms of normal brain development. Technical advances have also improved methods for identifying brain regions involved in developmental disorders, for tracing connections between parts of the brain, for visualizing individual neurons in living brain preparations, for recording the activities of neurons, and for studying the activity of single-ion channels and the receptors for various neurotransmitters. During the past 10 years the genetic basis of an ever increasing number of neurodevelopmental disorders has been discovered and has led to better understanding of the neurobiological basis of even common disorders such as global developmental delay, cerebral palsy, and autism. Current research should reveal their underlying molecular biology and eventually the possibility of targeted chemotherapy and the prevention of many neurodevelopmental disorders.
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Book chapters on the topic "Single-ion channel"

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Eaton, D. C., Y. Marunaka, and B. N. Ling. "Ion Channels in Epithelial Tissue: Single-Channel Properties." In Membrane Transport in Biology, 73–165. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-76983-2_3.

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Monyer, Hannah, and Peter Jonas. "Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices." In Single-Channel Recording, 357–73. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4419-1229-9_16.

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Manafirad, Arash. "Single Ion-Channel Analysis in Droplet Interface Bilayer." In Methods in Molecular Biology, 187–95. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0806-7_14.

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Pitts, Susan M. "Approximations for Semi-Markov Single Ion Channel Models." In Semi-Markov Models and Applications, 103–15. Boston, MA: Springer US, 1999. http://dx.doi.org/10.1007/978-1-4613-3288-6_6.

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Bezrukov, Sergey M., and John J. Kasianowicz. "Dynamic Partitioning of Neutral Polymers into a Single Ion Channel." In Structure and Dynamics of Confined Polymers, 117–30. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/978-94-010-0401-5_7.

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Lilienhof, H. J., and H. W. Hölscher. "Buried Single-Mode Channel Waveguides in BK 7 by Field Assisted Cs-Ion Exchange." In Springer Series in Optical Sciences, 71–74. Berlin, Heidelberg: Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-540-39452-5_17.

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Petersen, Ole. "Single-Channel and Whole-Cell Patch-Clamp Experiments on Gland Cells: Activation of Ion Channels Via Internal Messengers." In Cell Membrane Transport, 437–50. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4757-9601-8_22.

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Moczydlowski, Edward. "Single-Channel Enzymology." In Ion Channel Reconstitution, 75–113. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4757-1361-9_4.

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Fejtl, Michael, and David O. Carpenter. "Single-Channel Studies in Molluscan Neurons." In Ion Channels, 333–76. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1775-1_9.

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Colquhoun, David, and Alan G. Hawkes. "The Principles of the Stochastic Interpretation of Ion-Channel Mechanisms." In Single-Channel Recording, 397–482. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4419-1229-9_18.

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Conference papers on the topic "Single-ion channel"

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Fu-Chiang Young and Ching-Hsing Luo. "Model single ion channel for structure investigation." In 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering (NANOMED). IEEE, 2009. http://dx.doi.org/10.1109/nanomed.2009.5559107.

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Rossi, M., M. Bennati, F. Lodesani, S. Branchetti, and M. Tartagni. "A Compact System for Single Ion Channel Recording." In 2007 IEEE Sensors. IEEE, 2007. http://dx.doi.org/10.1109/icsens.2007.4388607.

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Huang, Zhongchao, Qiulin Tan, and Liqun Gu. "Restoring of single ion-channel current based on wavelet transform." In 2010 3rd International Congress on Image and Signal Processing (CISP). IEEE, 2010. http://dx.doi.org/10.1109/cisp.2010.5647800.

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Xiaowen Liu, Lin Li, and Andrew J. Mason. "High throughput single-ion-channel array microsystem with CMOS instrumentation." In 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2014. http://dx.doi.org/10.1109/embc.2014.6944196.

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Li, Haitao, Sina Parsnejad, and Andrew J. Mason. "Single ion channel CMOS electrochemical instrument for high throughput recording arrays." In 2015 IEEE 58th International Midwest Symposium on Circuits and Systems (MWSCAS). IEEE, 2015. http://dx.doi.org/10.1109/mwscas.2015.7282111.

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Kamimura, T., K. Yamamoto, and K. Matsumoto. "Ultra -low Energy Nitrogen -ion Irradiation for Improvement of Carbon Nanotube Channel Single Electron Transistor." In 2003 International Conference on Solid State Devices and Materials. The Japan Society of Applied Physics, 2003. http://dx.doi.org/10.7567/ssdm.2003.d-7-3.

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Li, Yonghong, Chaohui He, and Chunmei Xia. "Simulation of Heavy Ion Source-Drain Penetration Effect in SOI MOS and Bulk MOS." In 18th International Conference on Nuclear Engineering. ASMEDC, 2010. http://dx.doi.org/10.1115/icone18-29782.

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The Single Event Effects (SEE) of Silicon On Insulator (SOI) and bulk-silicon NMOS are simulated using the SENTAURUS-TCAD device simulator. The Source-Drain Penetration Effect, which is caused by a heavy ion, was shown. It is proved that when the feature size of device become less than a certain scale, both Direct Channel Effect and Indirect Channel Effect occur. By comparing the distributions of equipotential lines in the MOSFETs’ channels of the different feature size devices during the ion strikes indirectly, the Source-Drain-Penetration Effect occurs more evidently when the device feature size is getting smaller.
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Effertz, T., L. Becker, D. Beutner, and AJ Ricci. "Cell membrane lipids affect the mechano-electrical transduction channel. PIP2 (phosphatidylinositol-4,5-bisphosphat) specifically modulating single channel conductance and ion selectivity." In Abstract- und Posterband – 90. Jahresversammlung der Deutschen Gesellschaft für HNO-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn – Digitalisierung in der HNO-Heilkunde. Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-1686365.

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Schmidt, Tony, Rainer Schindl, Vedran Đerek, Linda Waldherr, Marta Nowakowska, Marie Jakešová, Muammer Üçal, et al. "Single light pulse stimulation of organic photocapacitors induces ion channel gating and action potentials in neurons." In Light Actuators for Optical Stimulation of Living Systems. València: FUNDACIO DE LA COMUNITAT VALENCIANA SCITO, 2022. http://dx.doi.org/10.29363/nanoge.liv-act.2022.001.

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Chu, Hon Fai, and Wai-Chee Shui. "Theoretical studies of AlGaAs-GaAs multiple-quantum-well single-channel waveguide defined by ion-implantation-induced intermixing." In Symposium on Integrated Optoelectronics, edited by Rolf H. Binder, Peter Blood, and Marek Osinski. SPIE, 2000. http://dx.doi.org/10.1117/12.391415.

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Reports on the topic "Single-ion channel"

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Shani, Uri, Lynn Dudley, Alon Ben-Gal, Menachem Moshelion, and Yajun Wu. Root Conductance, Root-soil Interface Water Potential, Water and Ion Channel Function, and Tissue Expression Profile as Affected by Environmental Conditions. United States Department of Agriculture, October 2007. http://dx.doi.org/10.32747/2007.7592119.bard.

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Constraints on water resources and the environment necessitate more efficient use of water. The key to efficient management is an understanding of the physical and physiological processes occurring in the soil-root hydraulic continuum.While both soil and plant leaf water potentials are well understood, modeled and measured, the root-soil interface where actual uptake processes occur has not been sufficiently studied. The water potential at the root-soil interface (yᵣₒₒₜ), determined by environmental conditions and by soil and plant hydraulic properties, serves as a boundary value in soil and plant uptake equations. In this work, we propose to 1) refine and implement a method for measuring yᵣₒₒₜ; 2) measure yᵣₒₒₜ, water uptake and root hydraulic conductivity for wild type tomato and Arabidopsis under varied q, K⁺, Na⁺ and Cl⁻ levels in the root zone; 3) verify the role of MIPs and ion channels response to q, K⁺ and Na⁺ levels in Arabidopsis and tomato; 4) study the relationships between yᵣₒₒₜ and root hydraulic conductivity for various crops representing important botanical and agricultural species, under conditions of varying soil types, water contents and salinity; and 5) integrate the above to water uptake term(s) to be implemented in models. We have made significant progress toward establishing the efficacy of the emittensiometer and on the molecular biology studies. We have added an additional method for measuring ψᵣₒₒₜ. High-frequency water application through the water source while the plant emerges and becomes established encourages roots to develop towards and into the water source itself. The yᵣₒₒₜ and yₛₒᵢₗ values reflected wetting and drying processes in the rhizosphere and in the bulk soil. Thus, yᵣₒₒₜ can be manipulated by changing irrigation level and frequency. An important and surprising finding resulting from the current research is the obtained yᵣₒₒₜ value. The yᵣₒₒₜ measured using the three different methods: emittensiometer, micro-tensiometer and MRI imaging in both sunflower, tomato and corn plants fell in the same range and were higher by one to three orders of magnitude from the values of -600 to -15,000 cm suggested in the literature. We have added additional information on the regulation of aquaporins and transporters at the transcript and protein levels, particularly under stress. Our preliminary results show that overexpression of one aquaporin gene in tomato dramatically increases its transpiration level (unpublished results). Based on this information, we started screening mutants for other aquaporin genes. During the feasibility testing year, we identified homozygous mutants for eight aquaporin genes, including six mutants for five of the PIP2 genes. Including the homozygous mutants directly available at the ABRC seed stock center, we now have mutants for 11 of the 19 aquaporin genes of interest. Currently, we are screening mutants for other aquaporin genes and ion transporter genes. Understanding plant water uptake under stress is essential for the further advancement of molecular plant stress tolerance work as well as for efficient use of water in agriculture. Virtually all of Israel’s agriculture and about 40% of US agriculture is made possible by irrigation. Both countries face increasing risk of water shortages as urban requirements grow. Both countries will have to find methods of protecting the soil resource while conserving water resources—goals that appear to be in direct conflict. The climate-plant-soil-water system is nonlinear with many feedback mechanisms. Conceptual plant uptake and growth models and mechanism-based computer-simulation models will be valuable tools in developing irrigation regimes and methods that maximize the efficiency of agricultural water. This proposal will contribute to the development of these models by providing critical information on water extraction by the plant that will result in improved predictions of both water requirements and crop yields. Plant water use and plant response to environmental conditions cannot possibly be understood by using the tools and language of a single scientific discipline. This proposal links the disciplines of soil physics and soil physical chemistry with plant physiology and molecular biology in order to correctly treat and understand the soil-plant interface in terms of integrated comprehension. Results from the project will contribute to a mechanistic understanding of the SPAC and will inspire continued multidisciplinary research.
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Schwindt, Peter C. Role of Spatially Distributed Ion Channels in Single Neuron Computation. Fort Belvoir, VA: Defense Technical Information Center, May 1993. http://dx.doi.org/10.21236/ada265972.

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Brozik, Susan Marie, Laura J. Douglas Frink, George David Bachand, David J. Keller, Elizabeth L. Patrick, Jason A. Marshall, Lauren A. Meyer, Ryan W. Davis, James A. Brozik, and Jeb Hunter Flemming. Integration of biological ion channels onto optically addressable micro-fluidic electrode arrays for single molecule characterization. Office of Scientific and Technical Information (OSTI), December 2004. http://dx.doi.org/10.2172/920735.

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Reid, M. S., X. Wang, N. Utting, and C. Jiang. Comparison of water chemistry of hydraulic-fracturing flowback water from two geological locations at the Duvernay Formation, Alberta, Canada. Natural Resources Canada/CMSS/Information Management, 2021. http://dx.doi.org/10.4095/329276.

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We analyzed and compared the water chemistry between 17 Fox Creek region samples, each from a different well, and 23 Three Hills region samples from a single well. Overall, the two regions were similar in chemical composition but showed small differences in some lower abundance dissolved elements. Additionally, we investigated changes in water chemistry of FPW over time from a single well. The majority of water quality parameters and water chemistry remained constant over the 7-month sampling time. Major ion chemistry showed increasing concentrations of Ca and Mg, and a decreasing concentration of SO4. Several trace elements also showed small trends of both increasing and decreasing concentrations over time. There was a strong correlation between Ca and Mg concentrations in both the Fox Creek region samples and Three Hills region samples, which is an indication of the mixing of formation water. However, the correlation between B and Sr was different among two region samples, which is likely due to the delayed mixing of formation water with the fracturing fluids during the flowback at different time periods of post fracturing. Likewise, Fox Creek region samples showed correlations between concentrations of Cl and Ca, Na and Ca, and Na and Mg, but these correlations were not seen in the Three Hills region samples. Geochemical modeling demonstrates that there are potential scales formed in the flowback water, but most of the minerals are still in the dissolution state in the formation. Stable isotopic analysis confirmed the mixing of injection water and the formation water.
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Hansen, Peter J., and Zvi Roth. Use of Oocyte and Embryo Survival Factors to Enhance Fertility of Heat-stressed Dairy Cattle. United States Department of Agriculture, August 2011. http://dx.doi.org/10.32747/2011.7697105.bard.

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The overall goal was to identify survival factors that can improve pregnancy success following insemination or embryo transfer in lactating dairy cows exposed to heat stress. First, we demonstrated that oocytes are actually damaged by elevated temperature in the summer. Then we tested two thermoprotective molecules for their effect on oocyte damage caused by heat shock. One molecule, ceramide was not thermoprptective. Another, insulin-like growth factor-1 (IGF) reduced the effects of heat shock on oocyte apoptosis and oocyte cleavage when added during maturation. We also used lactating cows exposed to heat stress to determine whether bovine somatotropin (bST), which increases IGF1 levels in vivo, would improve fertility in summer. Cows treated with bST received a single injection at 3 days before insemination. Controls received no additional treatment. Treatment with bST did not significantly increase the proportion of inseminated cows diagnosed pregnant although it was numerically greater for the bST group (24.2% vs 17.8%, 124–132 cows per group). There was a tendency (p =0.10) for a smaller percent of control cows to have high plasma progesterone concentrations (≥ 1 ng/ml) at Day 7 after insemination than for bST-treated cows (72.6 vs 81.1%). When only cows that were successfully synchronized were considered, the magnitude of the absolute difference in the percentage of inseminated cows that were diagnosed pregnant between bST and control cows was reduced (24.8 vs 22.4% pregnant for bST and control). Results failed to indicate a beneficial effect of bST treatment on fertility of lactating dairy cows. In another experiment, we found a tendency for addition of IGF1 to embryo culture medium to improve embryonic survival after embryo transfer when the experiment was done during heat stress but not when the experiment was done in the absence of heat stress. Another molecule tested, granulocyte-macrophage colony-stimulating factor (GM-CSF; also called colony-stimulating factor-2), improved embryonic survival in the absence of heat stress. We also examined whether heat shock affects the sperm cell. There was no effect of heat shock on sperm apoptosis (programmed cell death) or on sperm fertilizing ability. Therefore, effects of heat shock on sperm function after ejaculation if minimal. However, there were seasonal changes in sperm characteristics that indicates that some of the decrease in dairy cow fertility during the summer in Israel is due to using semen of inferior quality. Semen was collected from five representative bulls throughout the summer (August and September) and winter (December and January). There were seasonal differences in ion concentration in seminal plasma and in the mRNA for various ion channels known to be involved in acrosome reactions. Furthermore, the proportion of sperm cells with damaged acrosomes was higher in post-thaw semen collected in the summer than in its counterpart collected in winter (54.2 ± 3.5% vs. 51.4 ± 1.9%, respectively; P < 0.08Further examination is required to determine whether such alterations are involved in the low summer fertility of dairy cows.
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Mevarech, Moshe, Jeremy Bruenn, and Yigal Koltin. Virus Encoded Toxin of the Corn Smut Ustilago Maydis - Isolation of Receptors and Mapping Functional Domains. United States Department of Agriculture, September 1995. http://dx.doi.org/10.32747/1995.7613022.bard.

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Ustilago maydis is a fungal pathogen of maize. Some strains of U. maydis encode secreted polypeptide toxins capable of killing other susceptible strains of U. maydis. Resistance to the toxins is conferred by recessive nuclear genes. The toxins are encoded by genomic segments of resident double-strande RNA viruses. The best characterized toxin, KP6, is composed of two polypeptides, a and b, which are not covalently linked. It is encoded by P6M2 dsRNA, which has been cloned, sequenced and expressed in a variety of systems. In this study we have shown that the toxin acts on the membranes of sensitive cells and that both polypeptides are required for toxin activity. The toxin has been shown to function by creating new pores in the cell membrane and disrupting ion fluxes. The experiments performed on artificial phospholipid bilayers indicated that KP6 forms large voltage-independent, cation-selective channels. Experiments leading to the resolution of structure-function relationship of the toxin by in vitro analysis have been initiated. During the course of this research the collaboration also yielded X-ray diffracion data of the crystallized a polypeptide. The effect of the toxin on the pathogen has been shown to be receptor-mediated. A potential receptor protein, identified in membrane fractions of sensitive cells, was subjected to tryptic hydrolysis followed by amino-acid analysis. The peptides obtained were used to isolate a cDNA fragment by reverse PCR, which showed 30% sequence homology to the human HLA protein. Analysis of other toxins secreted by U. maydis, KP1 and KP4, have demonstrated that, unlike KP6, they are composed of a single polypeptide. Finally, KP6 has been expressed in transgenic tobacco plants, indicating that accurate processing by Kex2p-like activity occurs in plants as well. Using tobacco as a model system, we determined that active antifungal toxins can be synthesized and targeted to the outside of transgenic plant cells. If this methodology can be applied to other agronomically crop species, then U. maydis toxins may provide a novel means for biological control of pathogenic fungi.
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