Dissertations / Theses on the topic 'Short rotation forestry'

Despite the negative effects of fossil fuels on the environment, these remain as the primary contributors to the energy sector. In order to mitigate global warming risks, many countries aim at reducing greenhouse gas emissions. Bioenergy crops are being used as a substitute for fossil fuels and short rotation forestry is a prime example. In order to examine the sustainability of energy crops for fuel, typical European short rotation forestry (SRF) biomass, willow (Salix spp.) and poplar (Populus spp.) are examined and compared to rapeseed (Brassica napus L.) in respect to various aspects of soil respiration and combustion heat obtained from the extracted products per hectare. Various approaches are used to look at an As-contaminated site not only in the field but also in a soil-column experiment that examines the fate of trace elements in SRF soils, and in an analysis using MICMAC to describe the driving factors for SRF crop production. Based on the cause-effect chain, the impacts of land-use change and occupation on ecosystem quality are assessed when land-use is changed from degraded land (grassland) to willow and poplar SRF. A manual opaque dynamic closed chamber system (SEMACH-FG) was utilized to measure CO2 emissions at a willow/poplar short rotation forest in Krummenhennersdorf, Germany during the years 2013 and 2014, and at a rapeseed site in 2014. Short rotation forest soils showed higher CO2 emission rates during the growing season than the dormant season – with a CO2 release of 5.62±1.81 m-2 s-1 for willows and 5.08±1.37 µmol CO2 m-2 s-1 for poplars in the growing season. However, during the dormant season the soil sites with willow emitted 2.54±0.81 µmol CO2 m-2 s-1 and with poplar 2.07±0.56 µmol CO2 m-2 s-1. The highest emission rates for the studied plantations were observed in July for both years 2013 and 2014, during which the highest air and soil temperatures were recorded. Correlations between soil emission of CO2 and some meteorological parameters and leaf characteristics were investigated for the years 2013 and 2014. For example, for the willow clone (Jorr) and poplar clone (Max 3), high correlations were found for each between their soil emission of CO2 and both soil temperature and moisture content. Fitted models can explain about 77 and 75% of the results for Jorr and Max 3 clones, respectively. Moreover, a model of leaf area (LA) can explain about 68.6% of soil CO2 emission for H275. Estimated models can be used as a gap-filling method, when field data is not available. The ratio between soil respiration and the combustion heat calculated from the extracted products per hectare was evaluated and compared for the study’s willow, poplar and rapeseed crops. The results show that poplar and willow SRF has a very low ratio of 183 kg CO2 GJ 1 compared to rapeseed, 738 kg CO2 GJ 1. The soil-column experiment showed that by continuing the SRF plantation at the As-contaminated site, remediation would need only about 3% of the time needed if the site was left as a fallow field. In order to understand the complex willow and poplar short rotation forestry production system, 50 key variables were identified and prioritized to describe the system as a step to enhance the success of such potentially sustainable projects. The MICMAC approach was used in order to find the direct and the indirect relationships between those parameters and to classify them into different clusters depending on their driving force and interdependency. From this, it can be summarized that in order to enhance the success of a SRF system, decision makers should be focussing on: ensuring a developed wood-fuel market, increasing farmers’ experience/training, improving subsidy regulations and recommending a proper harvesting year cycle. Finally, the impacts of land-use change and occupation on the ecosystem quality were assessed. Results show that establishing SRF plantations on degraded lands improved the ecosystem structural quality (ESQ) by about 43% and ecosystem functional quality (EFQ) by about 12%. Based on overall results, poplar and willow SRF biomass can be recommended as renewable and sustainable sources for bioenergy.

Cette thèse s'est focalisée sur l'étude de la pertinence de l'utilisation des Taillis à Très Courte Rotation (TTCR) pour la production d'énergie via une gazéification, et sur la compréhension du comportement spécifique des TTCR lors de leur gazéification. Les TTCR ont une proportion d'écorce élevée conduisant à des propriétés particulières et potentiellement problématiques pour la gazéification. Les principales propriétés des TTCR, i. E. Leur composition en inorganiques et leur composition structurale, ont été mesurées sur des parties de plantes de TTCR d'eucalyptus et des fractions granulométriques de TTCR de peuplier. Le comportement des TTCR lors de la gazéification a ensuite été étudié. Dans un premier temps, la cinétique de torréfaction des TTCR a été étudiée par analyse thermogravimétrique (ATG), couplée à une analyse statistique pour analyser les corrélations avec les propriétés structurales. Les co-produits condensables de la torréfaction de TTCR de peuplier sont apparus globalement similaires quel que soit l'échantillon torréfié. Le taux élevé d'inorganiques dans les TTCR est susceptible d'influencer la gazéification du char, qui a donc été étudiée par ATG sous vapeur d'eau. Les caractéristiques cinétiques de la gazéification des TTCR ont été analysées statistiquement afin de modéliser l'impact catalytique des éléments inorganiques. L'étude des cendres au microscope électronique à balayage, couplé à un spectromètre X à dispersion d'énergie, a révélé la formation de silicates de potassium qui inhibent l'effet catalytique du potassium. Au regard de ces résultats, la gazéification des TTCR paraît réalisable pourvu qu'une préparation adaptée de la biomasse soit réalisée
In a context of increasing interest for bioenergy, this thesis focused on the opportunity of using short rotation forestry (SRF) feedstock for bioenergy production via the gasification process, and thus on the understanding of the specific gasification behavior of SRF. SRF feedstocks contain a large proportion of bark that impacts feedstock properties. The main characteristics of SRF, i. E. Inorganic composition and structural composition, were characterized on eucalyptus SRF plant parts and particle size fractions from poplar SRF. Larger particles were mainly composed of wood while particles below 0. 2 mm were fairly similar to bark. Then the behavior of SRF in gasification process was investigated. The focus was first put on torrefaction kinetics that was studied using thermogravimetric analysis (TGA), coupled with a statistical analysis to investigate the correlations with structural properties. Then the influence of the high inorganic content in SRF on the steam gasification of char was studied by TGA. The gasification kinetics data were processed with statistical tools to model the catalytic impact of inorganic elements. The characterization of gasification ash by scanning electron microscopy, coupled with energy-dispersive X-ray spectroscopy, showed that potassium silicates were formed that inhibited gasification kinetics. Based on these results, SRF feedstocks appeared to be suitable for gasification process, together with an adapted pretreatment of SRF feedstock

Short Rotation Forestry (SRF) has been introduced to the UK as a method to increase biomass production. However, some SRF species have raised concerns about potential impacts on the environment. A largely unknown aspect of SRF is the quality and quantity of leaf litter, and its impact on soil fauna, of which the earthworm community is a major component. Earthworms have direct impacts on the soil biogeochemistry of SRF systems, and the tree species can impact on the associated earthworm community. The aim of this study was to investigate the effects of SRF species and litter quality on earthworm communities, their diversity and activity. In addition, the effects of earthworms on SRF litter decomposition, carbon-nutrient cycling and tree growth were assessed. Field surveys, laboratory experiments and field experiments were utilised. Survey results suggested that SRF species, tree age, land-used history and soil type exhibited an interactive effect on overall earthworm community development. Further, growth of eucalyptus, as SRF on marginal-arable or reclaimed sites, led to relatively rapid earthworm colonisation and community development. SRF litter quality showed a direct effect on earthworm food selection, growth and reproduction. The native Alnus glutinosa, Betula pendula and Fraxinus excelsior litter supported earthworms and their activities over non-native Acer pseudoplatanus, Castaneas sativa and Eucalyptus nitens. Native British earthworms indicated a significant preference (p < 0.05) for E. nitens litter over A. pseudoplatanus and C. sativa. Earthworms showed a significant contribution (p < 0.05) to SRF litter decomposition, carbon and nutrient release within SRF systems and the degree of contribution varied with SRF species, earthworm density and diversity. Field studies demonstrated that a mixed earthworm community utilised non-native species but favoured particular native trees. Earthworm influence on nutrient uptake, tree growth and biomass production varied with SRF species. A one year field experiment showed that rapidly growing E. nitens benefited more from earthworm activity than relatively slow growing B. pendula. Overall, the current work supports the production of SRF, as with only one exception (C. sativa), results tended to show that SRF-earthworm interactions were positive. It is perhaps most interesting that non-native E. nitens showed a positive interaction with native British earthworms.

Fast growing hybrids of Salix and Populus can be grown in a short rotation coppice (SRC) system to produce renewable energy. This PhD investigates the interactions between the environment and productivity, with a view to finding the key limiting factors to yield and the potential of these crops to fulfil UK renewable energy obligations, now and in the future. An empirical modelling technique, using partial least squares regression was developed to extrapolate actual field observations to a national scale. Genotype x age x environment interactions were studied to examine the key limiting factors to productivity. Modelled yields differed between genotypes, with mean annual aboveground biomass ranging from 4.9 to 10.7 oven dry tonnes (odt) per hectare for Populus trichocarpa x P. deltoides genotype ‘Beaupré’ and Salix triandra x S. viminalis genotype ‘Q83’, respectively. Variation in yield was primarily described by spring and summer precipitation, suggesting water availability is the key limiting factor to yield. Output from the model was up-scaled across the UK using a geographic information system (GIS), and scenarios were developed to better understand the role and impact of land use management and policy development on potential crop distribution. For example, to meet UK biomass and biofuel targets without compromising food security or ecosystem services, would require 5 % of grade 3 land, 56 % grade 4 land and 47 % of grade 5 land. This quantity of biomass would produce 7.5 M tonnes of biomass per annum and would theoretically generate 15.5 TWh yr-1 of electrical energy, displacing 3.3 M tonnes of oil – approximately 4% of current UK electricity demand. The South West and North West alone producing over a third of this figure (5.2 TWh yr-1). These results suggest that SRC has the potential to become a significant component of a mixed portfolio of renewables. Furthermore, climate change is predicted to have far reaching consequences on crop growth. Process-based models can help quantify these interactions and predict future productivity. Here we use ForestGrowth-SRC, a process-based model originally designed for high-forest species and parameterised for a coppice system. Climate change scenarios (UK Climate Projections) were run with the model to assess the impact of a changing climate on the growth and spatial distribution of SRC poplar. Results suggest ForestGrowth-SRC is capable of accurately simulating growth over a large spatial and temporal scale. However, pests and disease were found to significantly affect yield. In the absence of pests and disease, productivity could increase by 20 % nationwide by 2080 (under a medium emissions scenario), suggesting we will see a future increase in the value and production of these crops as feedstocks for heat, power and liquid transportation fuels

The study focused on four research objectives: 1. To identify the species and provenances of eucalypts most suitable for biomass production in Great Britain. 2. To compare growth of eucalypts with other promising short rotation forestry genera. 3. To develop volume and biomass functions for E. gunnii. 4. To estimate yields and patterns of growth for E. gunnii. Searches on CAB abstracts and World of Science showed that there was limited research conducted on eucalypts in the UK. This research provides an original contribution to knowledge through; a long term assessment of the performance of species of cold tolerant eucalypts across a range of sites, identification of the basis for the rapid growth of eucalypts in comparison with trees from other genera, identification of the best fit function to describe stem form in E.gunnii and a characterisation of the pattern of growth in this species. The thesis provides an account of the long history of eucalypts in the UK, the first record of a eucalypt being planted in Britain probably being Eucalyptus obliqua in the late 1700s (Aiton 1789). A review is then provided of the experience and constraints to growing nine eucalypt species in the UK and their potential for short rotation forestry are described. The rapid growth of eucalypts makes them well suited to short rotation forestry, but there are considerable risks from frosts and extreme winters. Results from a trial established in Cumbria, north west England are described. Survival and growth was compared between E.gunnii, E. nitens and native or naturalised species, identified by Hardcastle (2006) as having potential for short rotation forestry. The rapid rate of growth of E. gunnii was attributed to a combination of large leaf area, a long period of growth during the year and a high specific leaf area. There was 99% mortality of E. nitens at the trial over winter, preventing comparison with other species. At the same trial and assessment was made of frost damage during the winter of 2009-2010, which proved to be the coldest for thirty years (Met Office 2010). E. gunnii was found to be more cold-tolerant than E. nitens, with 35% of the former surviving the winter and less than 1% of the latter. Larger trees were damaged more so than smaller trees reinforcing the argument for good silviculture to promote rapid, early growth. The study on stem form and growth of E. gunnii represents the first in the UK. Volume, height and dbh of a total of 636 trees, measured by felling, optical dendrometer and terrestrial laser scanner were used to test the goodness of fit of a volume function developed in France by AFOCEL and is South America by Shell Oil. The AFOCEL function was found to predict volume with less bias and be suitable for all but the smallest trees. Characterisation of growth curves using mined historic data indicated yields of 16 m3 ha-1 y-1 or approximately 8 t ha-1 y-1 at 20 years old. In contrast, growth curves derived from stem analysis of nine trees from Chiddingfold (south east England) and Glenbranter (central western Scotland) indicated lower yields at 7 m3 ha-1 y-1 at age 28 years and 4.5 m3 ha-1 y-1 at age 30 years respectively. Evidence from plantings elsewhere in the UK show that higher rates of growth are possible, but also that yields are often compromised by high mortality.

Worldwide there is an increasing demand of natural resources. In future, non renewable resources get substituted by renewable resources in the energetic sector as well as in the material sector. That implies a stronger usage of renewable resources especially - wood. In 2009 there was a usage of 77 million cubic meters of wood for material applications and a quantity of 55 million cubic meters for energetic applications in Germany alone. Furthermore, there is an increasing demand on wood for energetic purposes. In 2007 this problematic development led to the first supply bottlenecks. To meet the increasing demands of the future, Short Rotation Coppices (SRC) can help to improve the wood provision. An SRC is a planting of fast growing coppice on agricultural areas, which is managed more intensively than usual forestry practices for a quicker production of wooden biomass. With a comparative LCA of conventional wood and wood from SRC the present study evaluates if wood from SRC is reasonable to cover the increasing demand of wood for material and energetic purposes in an environmental friendly way. A comprehensive literature research regarding LCAs of wood and wooden products shows that there are no previous studies comparing the two types of wood. Hence, the present study examines a particleboard production as the material scenario and the combustion of woodchips in a firing system as the energetic scenario to compare the ecological advantages and disadvantages of wood from SRC and conventional wood. The LCA is implemented with the Gabi software designed by PE International. Data is obtained from previous LCA studies evaluating the production of wood, the particleboard production and the combustion of wood. Additionally, data from the Ecoinvent database is used. Functional units are the production of 1m3 particleboard and the production of 1 MJ of thermal energy. The LCIA is implemented with the “Ecoindicator” as endpoint- and “CML 2001” as midpoint approach to cover broad range of environmental issues. Moreover a sensitivity analyses shows the impact of decisive variables on the results of “Ecoindicator” and “CML 2001”. Results reveal that outcomes of the LCIA are dependent of the assessment method and the processed part of trees from conventional forestry. The present study shows, that with an efficient land use, wood from SRC can help to cover the increasing demand of wood for material and energetic purposes in a sustainable way. However, an immediate usage of wood for energetic purposes has to be seen critical. Instead, a cascaded and sustainable utilization of wood is recommendable to counteract climate change and to improve the efficient use of the renew-able resource - “wood”.

Worldwide there is an increasing demand of natural resources. In future, non renewable resources get substituted by renewable resources in the energetic sector as well as in the material sector. That implies a stronger usage of renewable resources especially - wood. In 2009 there was a usage of 77 million cubic meters of wood for material applications and a quantity of 55 million cubic meters for energetic applications in Germany alone. Furthermore, there is an increasing demand on wood for energetic purposes. In 2007 this problematic development led to the first supply bottlenecks. To meet the increasing demands of the future, Short Rotation Coppices (SRC) can help to improve the wood provision. An SRC is a planting of fast growing coppice on agricultural areas, which is managed more intensively than usual forestry practices for a quicker production of wooden biomass. With a comparative LCA of conventional wood and wood from SRC the present study evaluates if wood from SRC is reasonable to cover the increasing demand of wood for material and energetic purposes in an environmental friendly way. A comprehensive literature research regarding LCAs of wood and wooden products shows that there are no previous studies comparing the two types of wood. Hence, the present study examines a particleboard production as the material scenario and the combustion of woodchips in a firing system as the energetic scenario to compare the ecological advantages and disadvantages of wood from SRC and conventional wood. The LCA is implemented with the Gabi software designed by PE International. Data is obtained from previous LCA studies evaluating the production of wood, the particleboard production and the combustion of wood. Additionally, data from the Ecoinvent database is used. Functional units are the production of 1m3 particleboard and the production of 1 MJ of thermal energy. The LCIA is implemented with the “Ecoindicator” as endpoint- and “CML 2001” as midpoint approach to cover broad range of environmental issues. Moreover a sensitivity analyses shows the impact of decisive variables on the results of “Ecoindicator” and “CML 2001”. Results reveal that outcomes of the LCIA are dependent of the assessment method and the processed part of trees from conventional forestry. The present study shows, that with an efficient land use, wood from SRC can help to cover the increasing demand of wood for material and energetic purposes in a sustainable way. However, an immediate usage of wood for energetic purposes has to be seen critical. Instead, a cascaded and sustainable utilization of wood is recommendable to counteract climate change and to improve the efficient use of the renew-able resource - “wood”.

The purpose of this thesis is the determination of a minimum market price for Short-Rotation Forestry (SRF) willow biomass grown in monoculture and windbreaks in Quebec and Ontario. Full cost budgeting was used and developed on a Microsoft Excel spreadsheet. Harvest cycles of 3 and 4 years for monoculture plantations, and 8 years for windbreaks were investigated. Estimates for establishment and other preharvest costs were obtained from mid-sized (5 hectares) commercial SRF willow plots in Quebec and Ontario.
For the monoculture plantations, irrespective of cycle length, and using the base case figures, the final delivery cost of willow biomass ranged between 74-126 $/odMg and 63--109 $/odMg based on current and projected costs respectively. These hold for yields between 7 and 11 odMg/ha/yr. Moreover, the 4-year cycle was revealed to be more economically efficient than a 3-year cycle. Along with yield, the main cost factors affecting the economics of SRF monoculture plantations are: harvesting, transportation to a processing plant, land lease management. For the two major energy markets, ethanol and electricity production (biomass in replacement of coal), SRF willow biomass in monoculture plantations does not appear to be a viable feedstock given present technology and yields. In the short run, a more promising outlet for willow biomass is space heat production for small buildings, farm complexes, etc. In this market, many potential buyers can afford to pay a higher price for biomass than ethanol or electricity utilities.
In the windbreak system, biomass could be produced for between 44 and 68 $/odMg, assuming that yields between 12 to 20 odMg/ha/yr can be achieved. At this price, windbreak biomass is not a competitive feedstock for ethanol or electricity generation, but is attractive for space heat production. (Abstract shortened by UMI.)

Short Rotation Forestry (SRF) for bioenergy could be used to meet biomass requirements and contribute to achieving renewable energy targets. As an important source of biomass it is important to gain an understanding of the implications of large-scale application of SRF on the soil-atmosphere greenhouse gas (GHG) exchange. This study examined the effects of land use change (LUC) from grassland to SRF on soil fluxes of methane (CH4), nitrous oxide (N2O) and carbon dioxide (CO2), and the important drivers in action. Examining soils from a range of sites across the UK, CO2 emission potentials were reduced under SRF with differences between coniferous and broadleaved transitions; these changes were found to be related to changes in soil pH and microbial biomass. However, there were limited effects of SRF tree species type on CH4 and N2O fluxes. A detailed study at an experimental SRF site over 16 months demonstrated a reduction in CH4 and net CO2 emissions from soils under SRF and revealed intriguing temporal dynamics of N2O under Sitka spruce and common alder. A significant proportion of the variation in soil N2O fluxes was attributed to differences between tree species, water table depth, spatial effects, and their interactions. The effects of microtopography (ridges, troughs, flats), and its interactions with water table depth on soil GHG fluxes under different tree species was tested using mesocosm cores collected in the field. Microtopography did not significantly affect soil GHG fluxes but trends suggested that considering this spatial factor in sampling regimes could be important. N2O fluxes from Sitka spruce soils did not respond to water table depth manipulation in the laboratory suggesting that they may also be determined by tree-driven nitrogen (N) availability, with other research showing N deposition to be higher in coniferous plantations. An N addition experiment lead to increased N2O emissions with greatest relative response in the Sitka spruce soils. Overall, LUC from rough grassland to SRF resulted in a reduction in soil CH4 emissions, increased N2O emissions and a reduction or no change in net CO2 emissions. These changes in emissions were influenced both directly and indirectly by tree species type with Sitka spruce having the greatest effect on N2O in particular, thus highlighting the importance of considering soil N2O emissions in any life cycle analysis or GHG budgets of LUC to SRF for bioenergy. This research can help inform decisions around SRF tree species selection in future large-scale bioenergy planting.

[Truncated abstract] Insufficient water use by annual crop and pasture species leading to costly rises in saline watertables has prompted research into potentially profitable deep-rooted perennial species in the Western Australian wheatbelt. Native mallee eucalypts are currently being developed as a short-rotation coppice crop for production of leaf oils, activated carbon and bio-electricity for low rainfall areas (300—450 mm) too dry for many of the traditional timber and forage species. The research in this study was aimed at developing a knowledge base necessary to grow and manage coppiced mallee eucalypts for both high productivity and salinity control. This firstly necessitated identification of suitable species, climatic and site requirements favourable to rapid growth, and understanding of factors likely to affect yield of the desirable leaf oil constituent, 1,8-cineole. This was undertaken using nine mallee taxa at twelve sites with two harvest regimes. E. kochii subsp. plenissima emerged as showing promise in the central and northern wheatbelt, particularly at a deep acid sand site (Gn 2.61; Northcote, 1979), so further studies focussed on physiology of its resprouting, water use and water-use efficiency at a similar site near Kalannie. Young E. kochii trees were well equipped with large numbers of meristematic foci and adequate root starch reserves to endure repeated shoot removal. The cutting season and interval between cuts were then demonstrated to have a strong influence on productivity, since first-year coppice growth was slow and root systems appeared to cease in secondary growth during the first 1.5—2.5 years after cutting. After decapitation, trees altered their physiology to promote rapid replacement of shoots. Compared to uncut trees, leaves of coppices were formed with a low carbon content per unit area, and showed high stomatal conductance accompanied by high leaf photosynthetic rates. Whole-plant water use efficiency of coppiced trees was unusually high due to their fast relative growth rates associated with preferential investments of photosynthates into regenerating canopies rather than roots. Despite relatively small leaf areas on coppice shoots over the two years following decapitation, high leaf transpiration rates resulted in coppices using water at rates far in excess of that falling as rain on the tree belt area. Water budgets showed that 20 % of the study paddock would have been needed as 0—2 year coppices in 5 m wide twin-row belts in order to maintain hydrological balance over the study period. Maximum water use occurred where uncut trees were accessing a fresh perched aquifer, but where this was not present water budgets still showed transpiration of uncut trees occurring at rates equivalent to 3—4 times rainfall incident on the tree belt canopy. In this scenario, only 10 % of the paddock surface would have been required under 5 m wide tree belts to restore hydrological balance, but competition losses in adjacent pasture would have been greater

During the last decades, short rotation forestry (SRF) plantations have become attractive for Italian farmers due both to their capacity to produce large quantities of woody biomass for energy purposes and to the considerable amount of public incentives allowed. The present study quantifies in a chronosequence 52 plantations in the Italian region of Friuli Venezia Giulia in order to acquire extensive knowledge of the productivity potential of SRF plantations in Northern Italy, their mortality rate, shoot sprouting capacity after coppicing and above-ground biomass production. Two newly selected clones of hybrid poplar, Pegaso and Sirio, specific for biomass production were employed under intensive biennial coppice management, planted at 3,0 m x 0,5 m. Experimental plots were measured during the winter 2007/2008. Average yields during the first cutting cycle are of 5 Mg ha-1 year-1 of dry matter, and at the end of the second cutting cycle average yields are of 9 Mg ha-1 year-1 for both clones. Constraining factors were found in all plantation soils. Yields of SRF plantation are lower than the values reported in literature for experimental plots, but are similar to the results of SRF in marginal areas. In 38,5% of experimental plantations, yields are insufficient for economic purposes. In order to improve yield, SRF plantations need high input management like fertilization and irrigation. An alternative to chemical fertilization is disposing of animal farm effluents. A test on swine effluent disposal effects on SRF of poplar, willow and black locust was carried out in order to evaluate yields capacity under different effluent management. Black locust’s and willow’s yields increase after one treatment. Poplar does not show any yield differences under different effluent management, probably because of a nutrient excess. Swine effluent disposal could prove useful in increasing biomass yield, but should be calibrated on a species’ phytoextraction potential to avoid pollution.
Nel corso dell’ultimo decennio la SRF da biomassa ha iniziato a diffondersi in Italia grazie alla presenza di cospicui finanziamenti. Al fine di evidenziare le reali potenzialità produttive e le problematiche della SRF realizzata in pieno campo in impianti produttivi non sperimentali è stata condotta un’analisi sincronica in 52 aree di saggio in impianti di SRF realizzati in pieno campo in Friuli Venezia Giulia. Sono stati analizzati il tasso di mortalità, l’emissione di getti dalla ceppaia a seguito della ceduazione, le principali caratteristiche dendrometriche e qualitative dei getti di due cloni di pioppo selezionati per la produzione di biomassa, Sirio e Pegaso, al fine di determinarne la produzione di biomassa e la sua relazione con le caratteristiche pedologiche. Al termine del primo ciclo colturale è stata registrata un produzione media di sostanza secca di 5 Mg ha-1 anno-1, e di 9 Mg ha-1 anno-1 al termine del secondo ciclo colturale, valori che risultano in linea con quelli evidenziati per piantagioni condotte con modelli colturali caratterizzati da bassi input o su terreni caratterizzati da limitata fertilità stazionale, mentre rese sensibilmente maggiori sono riportate per impianti soggetti a fertilizzazione ed irrigazione. È stata quindi condotta una seconda sperimentazione in un impianto di SRF in provincia di Cuneo composto da tre specie, pioppo, robinia e salice, testando tre differenti tipologie di spandimento di refluo zootecnico, apportando elevati quantitativi di nutrienti. Le rese in termini di biomassa sono risultate essere elevate, ma il pioppo non ha risposto ai trattamenti con un incremento significativo delle rese, probabilmente a causa di un eccesso di nutrienti. Il salice e la robinia hanno presentato un aumento della produzione di biomassa nei trattamenti con i reflui rispetto le prove non trattate, a dimostrazione che apporti di nutrienti possano influenzare positivamente la resa della SRF, senza ulteriori aggravi di costi.

The establishment of intensively managed woody energy crops on reclaimed surface mine lands provides an opportunity to diversify domestic biomass sources, while increasing the productivity and economic value of underutilized land. Our objective is to test the effect of fertilization and irrigation on the growth, survival, biomass accumulation, biomass allocation, leaf area, and nutrient dynamics of American sycamore (Platanus occidentalis L.) and black locust (Robinia pseudoacacia L.) planted on a reclaimed surface mine. In 2008, replicated plantings of sycamore and black locust were established on the Big Elk mine in eastern Kentucky. Treatments tested include annual granular fertilizer applications of 37 kg N, 30 kg P, and 16 kg K ha-1, irrigation, irrigation + fertilization, and control. Following two growing seasons, American sycamore exhibited significantly (p < 0.05) greater height, diameter, leaf area, and stem biomass in fertilizer treatment compared to all other species and treatment combinations. Treatments had no affect on survival, but American sycamore exhibited significantly higher survival than black locust. Poor locust survival and growth were likely attributed to excessive ungulate browsing. Our findings indicate that fertilizer applications at young plantations on reclaimed mines in Appalachia increases tree height, diameter, and biomass accumulation.

Holz aus Kurzumtriebsplantagen (KUP) mit Pappeln (Populus spp.) kann eine bedeutende Rolle im Mix der Bioenergieressourcen in Deutschland spielen. Trotz eines günstigen Energieinput-Energieoutput-Verhältnisses ist das Erreichen wirtschaftlich zufriedenstellender Ergebnisse mit KUP jedoch auf den meisten potenziellen Anbauflächen durch hohe Ansprüche der Pappeln an die Wasserversorgung erschwert. Hohe Produktivitätsraten der Pappeln sind an einen hohen Wasserverbrauch gebunden und viele Trockenheitsanpassungen führen zu deutlichen Ertragsrückgängen. In der vorgestellten Arbeit wurde eine sechsjährige Vollgeschwister-F1-Kartierungspopulation der Europäischen Zitter Pappel (Aspe, Populus tremula L., Wuchsperiode 1998-2003) physiologisch und genetisch untersucht, um mögliche Wege zu einer züchterischen Verbesserung der Trockenheitstoleranz von Pappeln diskutieren zu können. Dabei wurde das Zuchtziel der Trockenheitstoleranz als Minimierung der Ertragsrückgänge unter trockenen Bedingungen definiert. Neben wuchsleistungsbezogenen Größen (Biomassegesamtleistung (BM, oberirdische Dendromasse), Biomassezuwachs (iBM), Radialzuwachs (ir), Baumhöhe (h)) wurden physiologisch holzanatomische Eigenschaften untersucht, die retrospektiv anhand der Jahrringe messbar sind und zur nicht direkt messbaren Eigenschaft der Trockenheitstoleranz in einer Beziehung stehen. Diese waren die Kohlenstoff- und Sauerstoffisotopsignatur (δ13C, δ18O), die Faser- und Gefäßgliedlänge (FL, GL), die Gefäßlumenquerschnittsfläche (AG), die Gefäßdichte (GD), der potenziell Saftstrom leitende Querschnittsflächenanteil (LQ), der hydraulisch gewichtete Gefäßlumendurchmesser (Dh) und die röntgendensitometrische Holzdichte (RD). Um trockenheitsbedingte physiologische Reaktionen vom Einfluss der Juvenilität der Kartierungspopulation unterscheiden zu können, wurden regionaltypische Juvenilitätstrends der RD, FL, GL, und des ir anhand eines zweiten, für das östliche Deutschland repräsentativen Aspen-Kollektivs aus natürlicher Sukzession ermittelt. Bedingt durch Trockenheitsanpassungen bzw. eine bevorzugte Wurzelentwicklung nach dem Verpflanzen zeigte die Kartierungspopulation in den ersten drei Jahrringen Abweichungen von den regionaltypischen Juvenilitätstrends. In den Trendverläufen der Kartierungspopulation heben sich die beiden Trockenvegetationsperioden 2000 und 2003 ab, wobei bis zum Sommer 2003 infolge des Starkniederschlages 2002 (Flut) ein außergewöhnlich gutes Grundwasserangebot herrschte. Alle untersuchten phänotypischen Eigenschaften zeigten 2000 starke trockenheitsbedingte Abweichungen. Im Jahrring 2003 wichen nur die GL und die RD von ihren Juvenilitätstrends ab. Außerdem konnte anhand der δ13C und δ18O Werte eine signifikante Abnahme der Wassernutzungseffizienz bzw. eine Zunahme der Transpiration im Jahr 2003 gezeigt werden. Die übrigen Größen folgten ihren Juvenilitätstrends und stiegen an. Die Jahrringdatensätze 2000 und 2003 der RD waren nicht signifikant mit der BM korreliert, dagegen zeigten die δ13C Datensätze 2002 und 2003 schwach positive Korrelationen mit der BM. Der trockenheitstoleranteste Genotyp verband seine überdurchschnittliche BM mit einer hohen Wassernutzungseffizienz (angezeigt durch überdurchschnittliche δ13C Werte), mit einer überdurchschnittlichen AG und mit einer nicht unterdurchschnittlichen RD in Höhe des Populationsmittels. Aufgrund des Fehlens negativer Korrelationen zwischen BM und δ13C bzw. BM und RD in der vorliegenden Arbeit können δ13C und RD als nützliche Weiser für die Unterscheidung der Trockenheitstoleranz verschiedener Aspen zu züchterischen Zwecken vorgeschlagen werden. Außer der BM unter trockenen Bedingungen kann keine der untersuchten Eigenschaften als alleiniger Trockenheitstoleranzweiser empfohlen werden. Zu einer Trockenheitstoleranzbewertung sollten Merkmalspaare verwendet werden, von denen ein Merkmal positiv mit dem Ertrag korreliert ist und das andere eine Trockenheitsanpassung verkörpert. Dadurch werden sowohl das primäre Zuchtziel eines höchstmöglichen Ertrages als auch eine bessere Trockenheitsangepasstheit berücksichtigt. Zwei verschiedene Trockenheitstoleranzindizierungen wurden angewendet, um die Kartierungsnachkommen entsprechend ihrer Trockenheitstoleranz einem Ranking zu unterziehen. Dabei wurden in beiden Fällen Bäume mit einem höheren Ertrag besser platziert. Der zweite Schwerpunkt der Arbeit lag auf der genetischen Kartierung von Quantitative Trait Loci (QTL) für die untersuchten phänotypischen Jahrringeigenschaften mit Bezug zur Trockenheitstoleranz. Als Basis für das QTL Mapping wurden, der Pseudo-Testcross-Mapping-Strategie folgend, genetische Kopplungskarten für die Elternbäume der Kartierungspopulation konstruiert. Die maternale Karte (P. tremula, „Schandau 4“) deckte mit 157 Markern (144 AFLP, 13 SSR) in 30 Kopplungsgruppen 1.369 cM ab, die 21 paternalen Kopplungsgruppen mit 148 Markern (132 AFLP, 16 SSR) überspannten 1.079 cM des Genoms (P. tremula, „Lichtenhain 1“). Die im Vergleich zur haploiden Chromosomenzahl der Pappeln (19) hohen Zahlen an Kopplungsgruppen sowie die hohen Zahlen an Doublets und unkartierten Markern zeigten eine geringe Genomabdeckung an. So konnte nur eine begrenzte Zahl, höchstens zwei QTL für die untersuchten phänotypischen Jahrringeigenschaften mit Bezug zur Trockenheitstoleranz, kartiert werden. Ein QTL Bereich mit pleiotropem Effekt auf mehrere wachstumsbezogene Größen wurde auf der maternalen Kopplungsgruppe 1 (dem Populus Chromosom I zuzuordnen) detektiert. Die Signifikanz der Effekte dieses QTL auf den Radialzuwachs entwickelte sich steigend mit zunehmendem Baumalter
Wood production in short rotation coppices (SRC) with poplar (Populus spp.) can contribute significantly to the future bio energy supply mix in Germany. Although the energy-input to energy-output ratio is rather good, SRC often do not meet cost effectiveness due to high water demand of poplar species. High biomass productivity depends on optimal water supply. Also, numerous adaptations to water deficits result in an undesirable decrease of yield. Combined physiological and genetic investigations were conducted within a six-year old F1-full-sib crossbred population of European aspen (Populus tremula L., growing period 1998-2003). Possible implications on selection, breeding or improvement of poplar cultivars showing a high tolerance to water deficits are discussed. For the work presented here, the breeding goal of higher water deficit tolerance was defined as the minimisation of yield losses under dry conditions. Beyond growth related traits (aggregate yield (BM), aboveground woody biomass), biomass increment (iBM) and radial increment (ir), physiological and wood anatomical traits were included; these are related to reactions to water deficit and are measurable on tree rings retrospectively. These traits were the Carbon- and Oxygen isotope ratios (δ13C, δ18O), the fibre length and vessel element length (FL, GL), the vessel lumen cross sectional area (AG), the vessel density (GD), the cumulative vessel lumen area to cross sectional area ratio (LQ), the hydraulically weighted mean vessel lumen diameter (Dh) and wood density assessed by X-ray densitometry (RD). To distinguish the drought induced physiologic reactions from juvenile developmental patterns of the mapping population, juvenile trends of RD, FL, GL, and ir, which are representative of habitats in south-eastern Germany, were investigated in a second aspen collective that was selected from natural succession. During the first three years, the mapping population showed deviations from the juvenile trends due to water deficit adaptations or preferential root development, respectively. Due to drought in the growing seasons of 2000 and 2003, the juvenile trends show outstanding values for both years, although ground water supply in 2003 was exceptionally good following the intense rain event of 2002 (Elbe flood 2002). The tree ring traits of both years stand out from the juvenile trends due to drought adaptations. In 2000, all phenotypic traits showed a significant deviation from their respective trends. In 2003, only GL and RD showed an adaptation to drought as observable by a deviation from their juvenile trends. A significant decrease in water use efficiency (WUE) and an increase in net transpiration, respectively, were shown for 2003 by means of δ13C or δ18O values. All other traits showed an increase following their juvenile trends. RD data for 2000 and 2003 were not significantly correlated with BM, but δ13C data (and therefore WUE) for 2002 and 2003 revealed a weakly positive correlation with BM. The genotype that was most tolerant to water deficits showed a combination of a superior growth with a superior WUE (by means of δ13C), a superior AG, and an RD close to but not less than the population average. Due to the lack of negative correlation between BM and δ13C or BM and RD in the present work, δ13C and RD can be valuable proxies for the determination of drought tolerance of aspen trees for tree improvement purposes. Aside from BM under dry conditions, no other traits that were investigated can be recommended as a stand-alone proxy for water deficit tolerance. For a water deficit tolerance evaluation, pairs of traits should be used, of which one trait is positively correlated with yield and the other represents a water deficit adaptation. Both the primary breeding goal of the highest possible yield as well as a better water deficit tolerance should always be considered in this context. Two different drought tolerance indices were used to rank the individuals of the mapping population according to their water deficit tolerance. In both cases, trees with higher BM were ranked better. The second focus of the present work is on genetic mapping of Quantitative Trait Loci (QTL) for the investigated tree-ring traits that refer to water deficit reaction. As a basis for the QTL-mapping approach, genetic linkage maps were constructed for each parental tree of the F1-full-sib crossbred mapping-population following the two-way pseudo-testcross mapping strategy. The maternal map (P. tremula, “Schandau 4”) consisted of 157 markers (144 AFLP, 13 SSR) in 30 linkage groups and covered 1,369 cM. The 21 linkage groups of the paternal map (P. tremula, “Lichtenhain 1”) covered 1,079 cM of the genome (144 AFLP, 13 SSR). Compared with the haploid chromosome number (19) of the Populus genome, the high number of linkage groups, doublets and unlinked markers indicated low genome coverage. Only a low number of QTL was detected, maximal two per in¬vesti¬gated phenotypic trait with a relation to water deficit tolerance. One QTL having a pleiotropic effect on several growth related traits was detected on the maternal linkage group 1 (corresponding to the Populus Chromosome I). The significance of the QTL effects seemed to increase with tree age

Student Number : 9906840R - MSc research report - School of Animal, Plant and Environmental Science
The global increased demand for forest products has led to an increase in the area of exotic fast-growing forest plantations. An understanding of nutrient cycling in plantations is essential to enhance their productivity. Sustainable forest productivity involves the managing of nutrients and genetic factors to maximize yields such that they are increasing or non-declining through the maintenance of soil quality and selection of superior tree species and breeds. Komatiland Forests Limited (KFL), a South African forestry company, initiated a permanent sampling plot (PSP) programme in 1998, where it monitors over 30 foliar and soil parameters, as well as tree growth parameters. This study utilized a subset of the permanent sample plots (PSPs) database to compile a suite of foliar and soil parameters that can be used to better interpret stand productivity in pine plantations. Data from PSPs of pine species Pinus patula, Pinus elliottii and the hybrid Pinus elliottii x caribaea planted on dolomite, granite and shale were used in the statistical analyses as they were well represented in the dataset. The geological analysis revealed that parent material significantly affects soil organic carbon content; soil exchangeable K, soil Fe, soil Mn and foliar Mn concentrations. Exchangeable K was found to be low across the geologies ranging on average from 0.08 – 0.11cmol/kg. An accumulative effect was found in foliar concentration of Mn across the geologies and species, with average foliar Mn concentrations being as high as 1086ppm. No statistically significant differences were found at the geological level in soil N, P, exchangeable Ca, Mg & Na, Al, pH or soil texture. Neither were there any significant differences in foliar concentration of N, P, Ca, Mg, Na, Cu, Fe, Zn, B and S at the geological level of analysis. However significant correlations were found between soil cation exchange capacity, soil pH and foliar concentration of Zn, Mn, Mg and Ca. Pinus patula had significantly higher foliar concentrations of N (p<0.001), P (p<0.001). Mg (p=0.001), B (p=0.001) and S (p<0.001) than the other pine variants under analysis. However when species x geology interaction analysis was used P. patula only had significantly higher foliar concentrations with regard to N (p<0.001) and P (p<0.001), and lower foliar concentrations of Zn (p<0.001) and Na (p=0.041) than the other pine variants under analysis. Across the species and geologies, soil acidification resulted in low Ca (0.15-1.6 cmol/kg) and Mg (0.1-0.7cmol/kg) availability. Positive and significant correlations were found between foliar and soil concentrations of N (p=0.022), P (p=0.030), Mg (p<0.001) and Ca (p<0.001). Productivity of the hybrid was significantly higher than the other two species (p<0.001), while P. elliottii had significantly lower productivity than P. patula (p=0.001). Regression models and a principal component analyses revealed that from the dataset of soil and foliar chemical and physical parameters Mg Soil, CEC, N soil, N foliar, P foliar, K foliar, Cu foliar, B foliar, S foliar, C:N soil, Ca:Al soil, N:Ca foliar, N:K foliar, clay and silt are best correlated with stand productivity.

Holz aus Kurzumtriebsplantagen (KUP) mit Pappeln (Populus spp.) kann eine bedeutende Rolle im Mix der Bioenergieressourcen in Deutschland spielen. Trotz eines günstigen Energieinput-Energieoutput-Verhältnisses ist das Erreichen wirtschaftlich zufriedenstellender Ergebnisse mit KUP jedoch auf den meisten potenziellen Anbauflächen durch hohe Ansprüche der Pappeln an die Wasserversorgung erschwert. Hohe Produktivitätsraten der Pappeln sind an einen hohen Wasserverbrauch gebunden und viele Trockenheitsanpassungen führen zu deutlichen Ertragsrückgängen. In der vorgestellten Arbeit wurde eine sechsjährige Vollgeschwister-F1-Kartierungspopulation der Europäischen Zitter Pappel (Aspe, Populus tremula L., Wuchsperiode 1998-2003) physiologisch und genetisch untersucht, um mögliche Wege zu einer züchterischen Verbesserung der Trockenheitstoleranz von Pappeln diskutieren zu können. Dabei wurde das Zuchtziel der Trockenheitstoleranz als Minimierung der Ertragsrückgänge unter trockenen Bedingungen definiert. Neben wuchsleistungsbezogenen Größen (Biomassegesamtleistung (BM, oberirdische Dendromasse), Biomassezuwachs (iBM), Radialzuwachs (ir), Baumhöhe (h)) wurden physiologisch holzanatomische Eigenschaften untersucht, die retrospektiv anhand der Jahrringe messbar sind und zur nicht direkt messbaren Eigenschaft der Trockenheitstoleranz in einer Beziehung stehen. Diese waren die Kohlenstoff- und Sauerstoffisotopsignatur (δ13C, δ18O), die Faser- und Gefäßgliedlänge (FL, GL), die Gefäßlumenquerschnittsfläche (AG), die Gefäßdichte (GD), der potenziell Saftstrom leitende Querschnittsflächenanteil (LQ), der hydraulisch gewichtete Gefäßlumendurchmesser (Dh) und die röntgendensitometrische Holzdichte (RD). Um trockenheitsbedingte physiologische Reaktionen vom Einfluss der Juvenilität der Kartierungspopulation unterscheiden zu können, wurden regionaltypische Juvenilitätstrends der RD, FL, GL, und des ir anhand eines zweiten, für das östliche Deutschland repräsentativen Aspen-Kollektivs aus natürlicher Sukzession ermittelt. Bedingt durch Trockenheitsanpassungen bzw. eine bevorzugte Wurzelentwicklung nach dem Verpflanzen zeigte die Kartierungspopulation in den ersten drei Jahrringen Abweichungen von den regionaltypischen Juvenilitätstrends. In den Trendverläufen der Kartierungspopulation heben sich die beiden Trockenvegetationsperioden 2000 und 2003 ab, wobei bis zum Sommer 2003 infolge des Starkniederschlages 2002 (Flut) ein außergewöhnlich gutes Grundwasserangebot herrschte. Alle untersuchten phänotypischen Eigenschaften zeigten 2000 starke trockenheitsbedingte Abweichungen. Im Jahrring 2003 wichen nur die GL und die RD von ihren Juvenilitätstrends ab. Außerdem konnte anhand der δ13C und δ18O Werte eine signifikante Abnahme der Wassernutzungseffizienz bzw. eine Zunahme der Transpiration im Jahr 2003 gezeigt werden. Die übrigen Größen folgten ihren Juvenilitätstrends und stiegen an. Die Jahrringdatensätze 2000 und 2003 der RD waren nicht signifikant mit der BM korreliert, dagegen zeigten die δ13C Datensätze 2002 und 2003 schwach positive Korrelationen mit der BM. Der trockenheitstoleranteste Genotyp verband seine überdurchschnittliche BM mit einer hohen Wassernutzungseffizienz (angezeigt durch überdurchschnittliche δ13C Werte), mit einer überdurchschnittlichen AG und mit einer nicht unterdurchschnittlichen RD in Höhe des Populationsmittels. Aufgrund des Fehlens negativer Korrelationen zwischen BM und δ13C bzw. BM und RD in der vorliegenden Arbeit können δ13C und RD als nützliche Weiser für die Unterscheidung der Trockenheitstoleranz verschiedener Aspen zu züchterischen Zwecken vorgeschlagen werden. Außer der BM unter trockenen Bedingungen kann keine der untersuchten Eigenschaften als alleiniger Trockenheitstoleranzweiser empfohlen werden. Zu einer Trockenheitstoleranzbewertung sollten Merkmalspaare verwendet werden, von denen ein Merkmal positiv mit dem Ertrag korreliert ist und das andere eine Trockenheitsanpassung verkörpert. Dadurch werden sowohl das primäre Zuchtziel eines höchstmöglichen Ertrages als auch eine bessere Trockenheitsangepasstheit berücksichtigt. Zwei verschiedene Trockenheitstoleranzindizierungen wurden angewendet, um die Kartierungsnachkommen entsprechend ihrer Trockenheitstoleranz einem Ranking zu unterziehen. Dabei wurden in beiden Fällen Bäume mit einem höheren Ertrag besser platziert. Der zweite Schwerpunkt der Arbeit lag auf der genetischen Kartierung von Quantitative Trait Loci (QTL) für die untersuchten phänotypischen Jahrringeigenschaften mit Bezug zur Trockenheitstoleranz. Als Basis für das QTL Mapping wurden, der Pseudo-Testcross-Mapping-Strategie folgend, genetische Kopplungskarten für die Elternbäume der Kartierungspopulation konstruiert. Die maternale Karte (P. tremula, „Schandau 4“) deckte mit 157 Markern (144 AFLP, 13 SSR) in 30 Kopplungsgruppen 1.369 cM ab, die 21 paternalen Kopplungsgruppen mit 148 Markern (132 AFLP, 16 SSR) überspannten 1.079 cM des Genoms (P. tremula, „Lichtenhain 1“). Die im Vergleich zur haploiden Chromosomenzahl der Pappeln (19) hohen Zahlen an Kopplungsgruppen sowie die hohen Zahlen an Doublets und unkartierten Markern zeigten eine geringe Genomabdeckung an. So konnte nur eine begrenzte Zahl, höchstens zwei QTL für die untersuchten phänotypischen Jahrringeigenschaften mit Bezug zur Trockenheitstoleranz, kartiert werden. Ein QTL Bereich mit pleiotropem Effekt auf mehrere wachstumsbezogene Größen wurde auf der maternalen Kopplungsgruppe 1 (dem Populus Chromosom I zuzuordnen) detektiert. Die Signifikanz der Effekte dieses QTL auf den Radialzuwachs entwickelte sich steigend mit zunehmendem Baumalter.
Wood production in short rotation coppices (SRC) with poplar (Populus spp.) can contribute significantly to the future bio energy supply mix in Germany. Although the energy-input to energy-output ratio is rather good, SRC often do not meet cost effectiveness due to high water demand of poplar species. High biomass productivity depends on optimal water supply. Also, numerous adaptations to water deficits result in an undesirable decrease of yield. Combined physiological and genetic investigations were conducted within a six-year old F1-full-sib crossbred population of European aspen (Populus tremula L., growing period 1998-2003). Possible implications on selection, breeding or improvement of poplar cultivars showing a high tolerance to water deficits are discussed. For the work presented here, the breeding goal of higher water deficit tolerance was defined as the minimisation of yield losses under dry conditions. Beyond growth related traits (aggregate yield (BM), aboveground woody biomass), biomass increment (iBM) and radial increment (ir), physiological and wood anatomical traits were included; these are related to reactions to water deficit and are measurable on tree rings retrospectively. These traits were the Carbon- and Oxygen isotope ratios (δ13C, δ18O), the fibre length and vessel element length (FL, GL), the vessel lumen cross sectional area (AG), the vessel density (GD), the cumulative vessel lumen area to cross sectional area ratio (LQ), the hydraulically weighted mean vessel lumen diameter (Dh) and wood density assessed by X-ray densitometry (RD). To distinguish the drought induced physiologic reactions from juvenile developmental patterns of the mapping population, juvenile trends of RD, FL, GL, and ir, which are representative of habitats in south-eastern Germany, were investigated in a second aspen collective that was selected from natural succession. During the first three years, the mapping population showed deviations from the juvenile trends due to water deficit adaptations or preferential root development, respectively. Due to drought in the growing seasons of 2000 and 2003, the juvenile trends show outstanding values for both years, although ground water supply in 2003 was exceptionally good following the intense rain event of 2002 (Elbe flood 2002). The tree ring traits of both years stand out from the juvenile trends due to drought adaptations. In 2000, all phenotypic traits showed a significant deviation from their respective trends. In 2003, only GL and RD showed an adaptation to drought as observable by a deviation from their juvenile trends. A significant decrease in water use efficiency (WUE) and an increase in net transpiration, respectively, were shown for 2003 by means of δ13C or δ18O values. All other traits showed an increase following their juvenile trends. RD data for 2000 and 2003 were not significantly correlated with BM, but δ13C data (and therefore WUE) for 2002 and 2003 revealed a weakly positive correlation with BM. The genotype that was most tolerant to water deficits showed a combination of a superior growth with a superior WUE (by means of δ13C), a superior AG, and an RD close to but not less than the population average. Due to the lack of negative correlation between BM and δ13C or BM and RD in the present work, δ13C and RD can be valuable proxies for the determination of drought tolerance of aspen trees for tree improvement purposes. Aside from BM under dry conditions, no other traits that were investigated can be recommended as a stand-alone proxy for water deficit tolerance. For a water deficit tolerance evaluation, pairs of traits should be used, of which one trait is positively correlated with yield and the other represents a water deficit adaptation. Both the primary breeding goal of the highest possible yield as well as a better water deficit tolerance should always be considered in this context. Two different drought tolerance indices were used to rank the individuals of the mapping population according to their water deficit tolerance. In both cases, trees with higher BM were ranked better. The second focus of the present work is on genetic mapping of Quantitative Trait Loci (QTL) for the investigated tree-ring traits that refer to water deficit reaction. As a basis for the QTL-mapping approach, genetic linkage maps were constructed for each parental tree of the F1-full-sib crossbred mapping-population following the two-way pseudo-testcross mapping strategy. The maternal map (P. tremula, “Schandau 4”) consisted of 157 markers (144 AFLP, 13 SSR) in 30 linkage groups and covered 1,369 cM. The 21 linkage groups of the paternal map (P. tremula, “Lichtenhain 1”) covered 1,079 cM of the genome (144 AFLP, 13 SSR). Compared with the haploid chromosome number (19) of the Populus genome, the high number of linkage groups, doublets and unlinked markers indicated low genome coverage. Only a low number of QTL was detected, maximal two per in¬vesti¬gated phenotypic trait with a relation to water deficit tolerance. One QTL having a pleiotropic effect on several growth related traits was detected on the maternal linkage group 1 (corresponding to the Populus Chromosome I). The significance of the QTL effects seemed to increase with tree age.