Relevant bibliographies by topics / Serum amino acids

Academic literature on the topic 'Serum amino acids'

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Published: 4 June 2021
Last updated: 20 February 2023

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Journal articles on the topic "Serum amino acids"

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Nagata, Yoko, Toyoaki Akino, and Kimiyoshi Ohno. "Microdetermination of serum d-amino acids." Analytical Biochemistry 150, no. 1 (October 1985): 238–42. http://dx.doi.org/10.1016/0003-2697(85)90465-8.

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Hamilton, Paul B. "ION EXCHANGE CHROMATOGRAPHY OF AMINO ACIDS - MICRODETERMINATION OF FREE AMINO ACIDS IN SERUM." Annals of the New York Academy of Sciences 102, no. 1 (December 15, 2006): 55–75. http://dx.doi.org/10.1111/j.1749-6632.1962.tb13625.x.

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Hawser, Stephen P., and Khalid Islam. "Binding of Candida albicans to Immobilized Amino Acids and Bovine Serum Albumin." Infection and Immunity 66, no. 1 (January 1, 1998): 140–44. http://dx.doi.org/10.1128/iai.66.1.140-144.1998.

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ABSTRACT In this study, we examined the binding of Candida albicans synchronized yeast-phase cells to plastic, immobilized amino acids and bovine serum albumin (BSA) and quantified the binding by using an XTT tetrazolium salt assay and absorbance determination. Our results show that C. albicans binds efficiently and specifically to several nonpolar aliphatic amino acids and positively charged amino acids and to BSA immobilized on tissue culture plastic but not to polar uncharged, negatively charged, or aromatic amino acids. Adhesion of yeasts to immobilized amino acids was not affected by preincubation of cells with BSA, whereas binding to immobilized BSA was affected by preincubation of yeasts with alanine, proline, and leucine but not by arginine or lysine. The ability to distinguish the chirality of these amino acids was also examined by using both thed and l amino acid configurations, and the results show that C. albicans yeasts recognize only thel configuration of these amino acids. The observations thatC. albicans specifically binds to certain amino acids indicate that these amino acids may prove useful tools for studying the binding interactions of C. albicans yeasts with host proteins such as components of the extracellular matrix.
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Łuszczewska-Sierakowska, Iwona, Marcin R. Tatara, Maria Szpetnar, and Jacek Kurzepa. "Free amino acid concentration in serum and trapezius muscle from male and female silver foxes (Vulpes vulpes)." Czech Journal of Animal Science 64, No. 3 (March 12, 2019): 130–40. http://dx.doi.org/10.17221/42/2018-cjas.

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Serum and muscle concentrations of 29 amino acids were determined in Silver fox. Serum concentrations of proline, alanine, tyrosine and aromatic amino acids were significantly higher in males than in females (all P = 0.05). Taurine and glycine concentrations in skeletal muscles were significantly higher in males than in females (P < 0.01). Muscle concentrations of cysteic acid, taurine, aspartate, threonine, serine, glycine, alanine, citruline, valine, leucine, gamma-amino-butyrate, ethanoloamine, lysine and histidine were significantly higher than in serum in both sexes (P < 0.05). In females, the concentrations of glutamate, glutamine, cystathionine, isoleucine, tyrosine, phenylalanine, arginine and amino-adipic acid were significantly higher in muscles than in serum (P < 0.05). Tryptophan concentration was significantly higher in serum from males than in muscles (P = 0.01). The concentration of branched-chain amino acids in skeletal muscles was approximately two times higher than in serum in both groups of foxes (P ≤ 0.01). Similar differences were obtained for aromatic amino acids in females (P = 0.04). The elaborated experimental model may serve for further studies focused on amino acid metabolism regulation in Canide and other monogastric mammals, especially with the use of environmental, dietary, pharmacological and toxicological factors. The elaborated experimental model may be an attractive alternative to replace some experiments on dogs.
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Blake, Amanda, Patricia Ishii, Robert Phillips, Jonathan Lidbury, Joerg Steiner, and Jan Suchodolski. "Analytical Validation of an Assay for Concurrent Measurement of Amino Acids in Dog Serum and Comparison of Amino Acid Concentrations between Whole Blood, Plasma, and Serum from Dogs." Metabolites 12, no. 10 (September 22, 2022): 891. http://dx.doi.org/10.3390/metabo12100891.

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Amino acids play an important role in metabolism. Comprehensive analytical validation of an assay for the concurrent measurement of a large number of amino acids in dogs is lacking, which precludes its usefulness in a clinical setting. Amino acids are often measured in plasma or whole blood. However, serum is commonly used for gastrointestinal diagnostic testing in dogs and is therefore convenient to use. This study aimed to analytically validate an assay for the concurrent measurement of amino acids in dog serum and to evaluate differences in amino acid concentrations in whole blood, plasma, and serum in dogs. Analytical validation of the assay (Biochrom 30+ Amino Acid Analyzer) was performed on fresh or banked serum samples from dogs. Whole blood, plasma, and serum from 36 healthy dogs were analyzed, and concentrations of the three sample types were compared. The assay was demonstrated to be precise, reproducible, accurate, linear, and stable for the measurement of the majority of compounds detected in dog serum. Cystine, glutamic acid, and ethanolamine were shown to be unstable at conditions commonly encountered in clinical settings. Significant differences in concentrations were identified between whole blood, plasma, and serum for 33 of 42 compounds. Amino acid profiles in serum and plasma were more similar to each other than to those in whole blood. While some amino acids are present in similar concentrations in whole blood, plasma, and serum, others are highly dependent on the type of biofluid, and measurements warrant strict adherence to sample type-based reference intervals.
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Niu, Yu-Cun, Ren-Nan Feng, Yan Hou, Kang Li, Zhen Kang, Jian Wang, Chang-Hao Sun, and Ying Li. "Histidine and arginine are associated with inflammation and oxidative stress in obese women." British Journal of Nutrition 108, no. 1 (October 14, 2011): 57–61. http://dx.doi.org/10.1017/s0007114511005289.

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The aims of the present study were to examine the serum amino acid profiles in obese and non-obese women and investigate the relationships between the serum amino acids and inflammation and oxidative stress in a human case–control study. Serum amino acids, inflammatory biomarkers (C-reactive protein and IL-6) and oxidative biomarkers (superoxide dismutase, malondialdehyde and glutathione peroxidase) were measured and compared in 235 obese women and 217 non-obese controls. The relationships between serum amino acids and inflammatory and oxidative biomarkers were examined using multiple linear regression. Among the amino acids determined, serum histidine, arginine, threonine, glycine, lysine and serine were found to be significantly lower in obese women as compared to non-obese controls (P < 0·001). The difference was the greatest for histidine (P < 0·001). In obese women, both histidine and arginine were negatively associated with inflammation and oxidative stress. In non-obese controls, histidine was negatively associated with oxidative stress. The findings in this study indicate that the metabolism of amino acids is abnormal in obese women in whom histidine and arginine have close relationships with inflammation and oxidative stress.
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Thompson, Dana K., Richard Sloane, James R. Bain, Robert D. Stevens, Christopher B. Newgard, Carl F. Pieper, and Virginia B. Kraus. "Daily variation of serum acylcarnitines and amino acids." Metabolomics 8, no. 4 (August 6, 2011): 556–65. http://dx.doi.org/10.1007/s11306-011-0345-9.

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Akahoshi, Noriyuki, Shotaro Kamata, Masashi Kubota, Takako Hishiki, Yoshiko Nagahata, Tomomi Matsuura, Chiho Yamazaki, et al. "Neutral aminoaciduria in cystathionine β-synthase-deficient mice, an animal model of homocystinuria." American Journal of Physiology-Renal Physiology 306, no. 12 (June 15, 2014): F1462—F1476. http://dx.doi.org/10.1152/ajprenal.00623.2013.

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The kidney is one of the major loci for the expression of cystathionine β-synthase (CBS) and cystathionine γ-lyase (CTH). While CBS-deficient ( Cbs−/−) mice display homocysteinemia/methioninemia and severe growth retardation, and rarely survive beyond the first 4 wk, CTH-deficient ( Cth−/−) mice show homocysteinemia/cystathioninemia but develop with no apparent abnormality. This study examined renal amino acid reabsorption in those mice. Although both 2-wk-old Cbs−/− and Cth−/− mice had normal renal architecture, their serum/urinary amino acid profiles largely differed from wild-type mice. The most striking feature was marked accumulation of Met and cystathionine in serum/urine/kidney samples of Cbs−/− and Cth−/− mice, respectively. Levels of some neutral amino acids (Val, Leu, Ile, and Tyr) that were not elevated in Cbs−/− serum were highly elevated in Cbs−/− urine, and urinary excretion of other neutral amino acids (except Met) was much higher than expected from their serum levels, demonstrating neutral aminoaciduria in Cbs−/− (not Cth−/−) mice. Because the bulk of neutral amino acids is absorbed via a B0AT1 transporter and Met has the highest substrate affinity for B0AT1 than other neutral amino acids, hypermethioninemia may cause hyperexcretion of neutral amino acids.
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Onozato, Mayu, Kana Kobata, Tatsuya Sakamoto, Hideaki Ichiba, and Takeshi Fukushima. "LC–MS/MS Analysis of Thiol-Containing Amino Acids in Exosomal Fraction of Serum." Journal of Chromatographic Science 58, no. 7 (June 24, 2020): 636–40. http://dx.doi.org/10.1093/chromsci/bmaa028.

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Abstract It has been suggested that thiol-containing amino acids could be used as biomarkers for diseases associated with oxidative stress. We investigated the thiol-containing amino acids, homocysteine (Hcy), cysteine (Cys), glutathione (GSH) and γ-glutamylcysteine (γ-GluCys), in commercial human serum by using liquid chromatography–tandem mass spectrometry (LC–MS/MS) after precolumn derivatization with 4-fluoro-7-sulfobenzofurazan. This method was applied to determine the composition of thiol-containing amino acids in exosomes prepared from the serum. Hcy, Cys, GSH and γ-GluCys could be detected in the exosomal fraction, and the ratio of each thiol-containing amino acid was similar to those in the corresponding native serum. Cys (94.76%) was most enriched in the exosomal fraction, followed by GSH (2.97%), γ-GluCys (1.59%) and Hcy (0.68%). These findings suggest that thiol-containing amino acids, Hcy, Cys, GSH and γ-GluCys, are included in exosomes in human serum.
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Fernstrom, M. H., E. A. Volk, and J. D. Fernstrom. "In vivo inhibition of tyrosine uptake into rat retina by large neutral but not acidic amino acids." American Journal of Physiology-Endocrinology and Metabolism 251, no. 4 (October 1, 1986): E393—E399. http://dx.doi.org/10.1152/ajpendo.1986.251.4.e393.

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The uptake of tyrosine into rat retina and brain was studied in vivo after its peripheral injection alone or in combination with other amino acids. Both retinal and brain tyrosine levels increased monotonically for at least 60 min after tyrosine administration. When tyrosine was injected along with branched-chain amino acids, but not with acidic amino acids, such increments in retinal and brain tyrosine levels were significantly attenuated. The postinjection tyrosine levels in retina and brain paralleled better the serum ratio of tyrosine to the sum of the other large neutral amino acids (which include the branched-chain amino acids) than the serum tyrosine level alone. These results suggest that tyrosine uptake into rat retina, like that into brain, is mediated by a competitive transport system shared among the large neutral amino acids.
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Dissertations / Theses on the topic "Serum amino acids"

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Mule, Hazarath Reddy Chiba Lee I. "The effect of early dietary amino acid restrictions on serum metabolites in pigs selected for lean growth efficiency." Auburn, Ala., 2005. http://hdl.handle.net/10415/1259.

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Kamalakar, Rajesh Babu Chiba Lee I. "Effect of the degree and duration of early dietary amino acid restrictions on growth performance, carcass traits, and serum metabolites of pigs, and physical and sensory characteristics of pork." Auburn, Ala, 2008. http://repo.lib.auburn.edu/EtdRoot/2008/FALL/Animal_Sciences/Thesis/Kamalakar_Rajesh_17.pdf.

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McGregor, Neil Roland. "An investigation of the association between toxin producing staphylococcus, biochemical changes and jaw muscle pain." University of Sydney. Prosthetic Dentistry, 2000. http://hdl.handle.net/2123/369.

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Objectives: To assess the expression of the symptoms of jaw muscle pain and its association with alterations in biochemistry, other symptoms and the carriage of staphylococci. Methods: Three different study populations were assessed. The first was selected and examined by the author and consisted of 43 pain and 41 age and sex matched controls. The second was a study of CFS patients who were blinded to the author and the author subsequently examined the associations between jaw muscle symptom reporting and the standardised biochemistry measures. The third study was also blinded to the author but included an investigation of staphylococci and certain cytokine and biochemistry measures. Results: The three studies clearly establish an association between the carriage of toxicogenic coagulase negative staphylococci and the expression of jaw muscle pain in both males and females. These associations were homogeneous and were found whether the patients were selected on the basis of having jaw muscle pain or selected from within a population of patients selected on the basis of having Chronic Fatigue Syndrome. The studies associated the changes with variations in biochemistry and these were in turn associated with symptom expression within the jaw muscle pain patients. These biochemical alterations included the dysregulation of immune cell counts, cytokines, electrolyte and protein metabolism. These symptoms and biochemical changes were associated with pain severity and illness duration and staphylococcal toxin production. From the data a model was developed which shows the mechanisms involved in the development of chronic pain in the jaw muscles. Conclusions: The carriage of toxicogenic coagulase-negative staphylococci were found to be associated with the expression of jaw muscle pain and the alterations in biochemistry associated with these symptoms.
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McGregor, Neil Roland. "An investigation of the association between toxin producing staphylococcus, biochemical changes and jaw muscle pain." Thesis, The University of Sydney, 1999. http://hdl.handle.net/2123/369.

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Objectives: To assess the expression of the symptoms of jaw muscle pain and its association with alterations in biochemistry, other symptoms and the carriage of staphylococci. Methods: Three different study populations were assessed. The first was selected and examined by the author and consisted of 43 pain and 41 age and sex matched controls. The second was a study of CFS patients who were blinded to the author and the author subsequently examined the associations between jaw muscle symptom reporting and the standardised biochemistry measures. The third study was also blinded to the author but included an investigation of staphylococci and certain cytokine and biochemistry measures. Results: The three studies clearly establish an association between the carriage of toxicogenic coagulase negative staphylococci and the expression of jaw muscle pain in both males and females. These associations were homogeneous and were found whether the patients were selected on the basis of having jaw muscle pain or selected from within a population of patients selected on the basis of having Chronic Fatigue Syndrome. The studies associated the changes with variations in biochemistry and these were in turn associated with symptom expression within the jaw muscle pain patients. These biochemical alterations included the dysregulation of immune cell counts, cytokines, electrolyte and protein metabolism. These symptoms and biochemical changes were associated with pain severity and illness duration and staphylococcal toxin production. From the data a model was developed which shows the mechanisms involved in the development of chronic pain in the jaw muscles. Conclusions: The carriage of toxicogenic coagulase-negative staphylococci were found to be associated with the expression of jaw muscle pain and the alterations in biochemistry associated with these symptoms.
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Hernandez, Cid Aaron. "New NMR methods for mixture analysis." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/new-nmr-methods-for-mixture-analysis(8ee7b062-e1cf-4c31-9a4b-3e6603b2d29c).html.

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This thesis is focussed on the investigation of matrices for matrix-assisted diffusion-ordered spectroscopy (MAD). Diffusion-ordered spectroscopy (DOSY) is a family of experiments where the resonances in the chemical shift dimension are further dispersed in an extra dimension according to diffusion coefficient. A typical DOSY spectrum shows one single diffusion coefficient for all the resonances coming from one single species. However, If two or more resonances overlap, the diffusion resolution of the DOSY spectrum is compromised and a spurious diffusion coefficient results, intermediate between the species. In case of signal overlap, the use of more advanced processing methods aids to separate two analytes that differ by at least 30% in diffusion coefficient. In practice, many mixtures contain species of similar diffusion coefficients whose resonances overlap in the chemical shift dimension. The addition of co-solutes can modify the chemical environment (matrix), with which different analytes interact to different extents, and enhance the diffusion resolution of DOSY. However, the addition of co-solutes can risk the benefits of DOSY by increasing the probability of signal overlap. Signal overlap in MAD is avoided by using a 1H NMR-invisible surfactant such as sodium perfluorooctanoate (NaPFO), which has replaced each proton by a fluorine atom. PFO micelles are a tunable matrix which allows the separation of analytes via coulombic interactions by adjusting the pH. Differences in diffusion coefficient in NaPFO solution can be analysed using a modified Lindman's law to model the diffusion coefficient as a function of pH. The model rationalises the binding constants of analytes to PFO micelles with good accuracy, subject to the spectral data quality. Another alternative to resolve diffusion coefficients using the invisible MAD approach is by means of a commercially available alkyl surfactant like cetyltrimethylammonium bromide (CTAB). CTAB in high ionic strength solution forms worm-like micelles whose resonances can be filtered out from the final DOSY spectrum. CTAB worm-like micelles have short transverse relaxation times compared to all of the analytes in the mixture. If a transverse relaxation filter is positioned at the beginning of a standard DOSY pulse sequence, as in PROJECT-Oneshot, the strong CTAB signals vanish and leave behind only the analyte resonances and hence avoid signal overlap. Finally, the use of bovine serum albumin (BSA) as a potential invisible matrix, using a similar approach to CTAB worm-like micelles is investigated, using a relaxation-weighted DOSY pulse sequence to suppress most of the BSA background signal (at a cost in analyte signal to noise ratio). An alternative to suppress most of the BSA background and preserve most of the analyte signal is by means of mild transverse relaxation filtration and spectral editing to obtain an edited DOSY spectrum that shows only the analyte signals. Nonetheless, it is a shame that useful MAD results can only be obtained under a narrow set of conditions: i) different mole ratios BSA: analyte to aid diffusion resolution, ii) mild T2 filtration to improve analyte signal to noise ratio and iii) spectral editing to remove residual BSA background.
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Mariusso, Ana Carolina Bossi. "Estudo do enriquecimento de massas alimenticias com subprodutos agroindustriais visanto melhoramento funcional e tecnologico de massas frescas." [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256705.

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Orientador: Glaucia Maria Pastore
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
Made available in DSpace on 2018-08-11T05:24:27Z (GMT). No. of bitstreams: 1 Mariusso_AnaCarolinaBossi_M.pdf: 451123 bytes, checksum: 21c6b468642fe226f7319a3edcd7cd1a (MD5) Previous issue date: 2008
Resumo: Há na atualidade uma grande preocupação em atender às necessidades nutricionais da população brasileira como melhoria do ¿status¿ de saúde. A Indústria de Alimentos Brasileira tem uma grande importância no contexto socioeconômico do país e produz uma variedade de subprodutos tais como: soro de leite, extrato de levedura e farinha de soja, sem qualquer utilização mais nobre que não seja produção de rações ou queima para gerar energia. Este trabalho teve como objetivo propor um alimento de baixo custo, de grande aceitação e com um valor nutritivo alto, usando subprodutos das indústrias de alimentos para um melhora nas condições de saúde da população, especialmente da população carente, principalmente das crianças que se alimentam na escola. As massas alimentícias, sendo uma das formas mais antigas de alimentação, são também muito versáteis, tanto do ponto de vista nutricional quanto do ponto de vista gastronômico, podendo ser preparadas e servidas de formas diversas. É por este motivo que se propõe a utilização da massa para a produção de um alimento de alto valor protéico. Foram analisadas as características físicas, físico-químicas e sensoriais do macarrão tradicional e nos macarrões enriquecidos com soro de leite em pó, extrato de levedura e farinha de soja desengordurada. Foi verificado, através dos resultados obtidos, que as misturas acrescidas com 9% de farinha de soja desengordurada apresentaram melhor comportamento, sendo, portanto um produto de boa qualidade nutricional, boa aceitabilidade geral (sabor, odor, textura, cor e consistência) e baixo custo. Neste contexto, conclui-se que o enriquecimento do produto desenvolvido com farinha de soja desengordurada é viável tecnicamente oferecendo uma excelente alternativa para a fabricação do macarrão fresco. O acréscimo de proteínas apresentou vantagens quantitativas e qualitativas ao produto desenvolvido quando comparado ao produto tradicional, o qual tem alta concentração de carboidratos e baixo teor de proteínas
Abstract: There is currently a major concern to meet the nutritional needs of the Brazilian population as improving the "status" of health. The Brazilian Food Industry has a very important socio-economic context in the country and produces a variety of products such as serum of milk, extract, yeast and flour, soybeans, without any use more noble than production of feed or burning to generate energy. This work aims to propose a low cost food with high acceptance and high nutritional value, for an improvement in the health conditions of poor population, mainly children who have meals at school. For this reason it was chosen the pasta, as it is one of the oldest recipes in history, it is also very versatile, regarding nutritional and gastronomic aspect and, can be prepared and served in a many ways. For this reason it¿s proposed the use of pasta for the production of a high value food protein. There has been analyzed the physical, physical-chemical and sensory of traditional pasta and also pasta fortified with whey milk powder, yeast extract, and defatted soybean. Flour. It has been verified through obtained results, that the mixes with 9% of defatted soy flour, had the proportion which presented the best effects, being therefore a product of good nutrition, good general acceptance (taste, appearance, color, smell, consistency) and low cost. In this context, it was concluded that the enrichment of the product developed with, defatted soy flour is technically feasible offering an excellent alternative for the manufacture of fresh pasta. The addition of protein presented quantitative and qualitative advantages of the product developed compared to the traditional product, which has mainly carbohydrates, and low content of protein
Mestrado
Mestre em Ciência de Alimentos
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Rajamanickam, Jeyaganesh. "The serum and glucocorticoid inducible kinase in the regulation of sodium coupled amino acid transporters." [S.l. : s.n.], 2007.

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Naruse(Miura), Midori. "Effects of taste stimulation on the behavior of serum amino acid concentrations and amylase and trypsin activities in fasting rats." Kyoto University, 2001. http://hdl.handle.net/2433/150203.

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Nelson, Geoffrey Winston. "Surface characterization and functional properties of carbon-based materials." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:f22b95ce-65f3-4d9e-ac3d-a88f6e142c1a.

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Carbon-based materials are poised to be an important class of 21st century materials, for bio-medical, bio-electronic, and bio-sensing applications. Diamond and polymers are two examples of carbon-based materials of high interest to the bio-materials community. Diamond, in its conductive form, can be used as an electrochemical bio-sensor, whilst its nanoparticle form is considered a non-inflammatory platform to deliver drugs or to grow neuronal cells. Polymers, especially when chemically modified, have been used extensively in biological environments, from anti-microbial use to drug delivery. The large-scale use of either material for biological use is limited by two factors: ease of chemical modification and the paucity of knowledge of their surface chemistry in aqueous media. This thesis addresses aspects of both these issues. The first study reported is an in situ study of the adsorption dynamics of an exemplar globular protein (bovine serum albumin, BSA) on nanodiamond using the relatively novel quartz crystal microbalance with dissipation (QCM-D) technique. For the first time, QCM-D enabled the detailed study of protein dynamics (i.e. kinetics, viscoelastic properties, overlayer structure, etc.) onto nanodiamond thin films having various surface chemistry and roughness. The dynamics of protein adsorption is found to be sensitive to surface chemistry at all stages of adsorption, but it is only sensitive to surface roughness during initial adsorption phases. Our understanding of the nanodiamond-biology interface is enhanced by this study, and it suggests that QCM-D is useful for the study of the surface chemistry of nanoparticle forms of inorganic materials. A second study concerns a novel surface functionalization scheme, based on carbene and azo-coupling chemistry, which has been recently introduced as a practical, facile method for modifying the surfaces of polymers. Using modern surface characterization techniques, it is demonstrated that a chemical linker can be attached to polystyrene surfaces using carbene-based chemistry, and that further chemical functionality can be added to this chemical linker via an azo-coupling reaction. In situ studies of protein dynamics at these interfaces were conducted using QCM-D, thus enabling a link between specific protein behaviour and the polymer surface chemical termination chemistry to be made. A third area of study of investigates the use of diamond electrodes as a bio-sensor for dopamine under physiological conditions. For these conditions, ascorbic acid interferes with the dopamine oxidation signal, in ways that render the two signals irresolvable. Various modifications are used in attempts to reduce this interference, including: small and large cathodic treatments, grafting of electro-active polymers, addition of carbon nanotubes, and hydrogen plasma treatment. Those modifications leading to the hydrogen-termination of diamond are shown to work the best. Notably, hydrogen plasma treatment effects the complete electrochemical separation of dopamine and ascorbic acid at a diamond electrode. This is the first time this has been accomplished without adding non-diamond materials to the diamond electrode surface.
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Chang, Cheng Fang, and 李金益. "Dynamics of Serum Free Amino Acid in Marine Shrimp Penaeus." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/73845489567541743053.

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碩士
國立中山大學
海洋生物研究所
81
Dynamics of free amino acids in the serum of Penaeus odon were studied. Two experiments were conducted.the first experiment, the shrimp(15-20g) were fedstalline arginine or cellulose acetate phthalateroencapsulated arginine . In the second one, fishmealplemented with either crystalline or encapsulatedinine were compared. Diet was giving by force feedingng a micropipet. Hemolymph of individual shrimp washdrawn repeatedly for amino acid analysis. Theult indicated that microencapsulation significantlyreased (up to 10 times of that crystalline arginine groupprolonged (at least 8 hr vs. 3 hr) the serum argininecentration. Supplementation to fishmeal of arginine alsowed similar patterns. The results strongly suggest thatinability of the shrimp to utilize crystalline aminod is caused by the poor absorption of crystalline aminods. Microencapsulation not only improve the absorptionalso prolong the duration time of high argininecentration in the serum. Serum ornithine concentrationreased with serum arginine and thus indicates that itht be the major metabolite when serum arginine was high. ithine is probably metabolized further in the urea cycle.
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Books on the topic "Serum amino acids"

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Pearl, Phillip L., and William P. Welch. Pediatric Neurotransmitter Disorders. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780199937837.003.0059.

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The pediatric neurotransmitter disorders represent an enlarging group of neurological syndromes characterized by inherited abnormalities of neurotransmitter synthesis, metabolism, and transport. Disorders involving monoamine synthesis include guanosine triphosphate cyclohydrolase deficiency (Segawa disease or classical Dopa-responsive dystonia as the heterozygous form), aromatic amino acid decarboxylase deficiency, tyrosine hydrolase deficiency, sepiapterin reductase deficiency, and disorders of tetrahydrobiopterin synthesis. These disorders can be classified according to whether they feature elevated serum levels of phenylalanine. Disorders of γ-amino butyric acid (GABA) metabolism include succinic semialdehyde dehydrogenase deficiency and GABA-transaminase deficiency. Glycine encephalopathy is typically manifested by refractory neonatal seizures due to a defect in the glycine degradative pathway. Pyridoxine-responsive seizures have now been associated with deficiency of α-aminoadipic semialdehyde dehydrogenase as well as a variants requiring therapy with pyridoxal-5-phosphate and folinic acid.
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Book chapters on the topic "Serum amino acids"

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Baccino, E., J. Caroff, J. C. Bigot, J. F. Menez, and H. H. Floch. "Is serum tryptophan a good indicator of CNS serotonin metabolism in chronic alcoholics with hepatopathy?" In Amino Acids, 858–60. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-011-2262-7_104.

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Claes, M., M. Maes, L. Delbeke, C. Schotte, Y. Jacquemyn, and S. Scharpe. "The relationships between the availability of L-tryptophan in serum and HPA-axis function in postpartum females." In Amino Acids, 1035–39. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-011-2262-7_129.

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Stepuro, I. I., and N. A. Chaikovskaya. "Interaction of glycosylated amino acids with human serum albumin." In Spectroscopy of Biological Molecules: New Directions, 39–40. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4479-7_17.

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Hemminki, K. "Binding of Styrene Oxide to Amino Acids, Human Serum Proteins and Hemoglobin." In Archives of Toxicology, 286–90. Berlin, Heidelberg: Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-71248-7_46.

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Cappelli, P., K. Langer, M. Evangelista, P. F. Palmieri, S. Marzan, G. del Rosso, B. Di Paolo, and A. Albertazzi. "Serum Branch-Chain Amino and Keto Acids in the Nutritional and Immunological Assessment of Uremics on Conservative and Dialysis Treatment." In Current Therapy in Nephrology, 275–77. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4613-0865-2_73.

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Moorad, Deborah R., Gregory E. Garcia, and B. P. Doctor. "Amino Acid Sequence of Horse Serum Butyrylcholinesterase." In Structure and Function of Cholinesterases and Related Proteins, 145–46. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4899-1540-5_37.

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Davison, A. S., B. P. Norman, E. A. Smith, J. Devine, J. Usher, A. T. Hughes, M. Khedr, A. M. Milan, J. A. Gallagher, and L. R. Ranganath. "Serum Amino Acid Profiling in Patients with Alkaptonuria Before and After Treatment with Nitisinone." In JIMD Reports, 109–17. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/8904_2018_109.

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Fernández-Quintela, A., M. T. Macarulla, A. S. Del Barrio, and J. A. Martinez. "Serum Amino Acid Profile and Protein Utilization in Rats Fed on a Pea Protein Isolate." In Plant Proteins from European Crops, 203–8. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-662-03720-1_35.

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Miyazaki, Teruo, Hironori Nagasaka, Haruki Komatsu, Ayano Inui, Ichiro Morioka, Hirokazu Tsukahara, Shunsaku Kaji, et al. "Serum Amino Acid Profiling in Citrin-Deficient Children Exhibiting Normal Liver Function During the Apparently Healthy Period." In JIMD Reports, 53–61. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/8904_2018_99.

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Egashira, Yukari, Makiko Sato, Atsushi Tanabe, Kuniaki Saito, Suwako Fujigaki, and Hiroo Sanada. "Dietary Linoleic Acid Suppresses Gene Expression of Rat Liver α-Amino-β-Carboxymuconate-ε-Semialdehyde Decarboxylase (ACMSD) and Increases Quinolinic Acid in Serum." In Advances in Experimental Medicine and Biology, 671–74. Boston, MA: Springer US, 2003. http://dx.doi.org/10.1007/978-1-4615-0135-0_79.

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Conference papers on the topic "Serum amino acids"

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ISMAILOV, Ismail Sagidovich, Nina Vladimirovna TREGUBOVA, Rashid Hasanbievich KOCHKAROV, Anna Victorovna MORGUNOVA, and Natalija Alecseevna DRIZHD. "THE RELATIONSHEEP OF AMINO ACID METABOLISM WITH PRODUCTIVITY OF GROWING YOUNG SHEEP." In RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.125.

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A number of studies on digestion of the ruminants have shown the process of synthesis of all the essential amino acids in the rumen (Abu Fadel, 2004; Trukhachev, V., Zlidnev, N. 2008). However, according to some researches, microbial protein is deficient in methionine and lysine. This assumption also proves the need for further study of the influence of balanced amino acids to these diets on the growth, development and productive performance of the ruminants. Scientific approbation of the issues related to establishing the requirements of young sheep in lysine and methionine with cystine and development of recommendations for their proper balance in animal rations have been conducted along with a series of physiological, scientific and practical experiments. For this purpose, we used the following research methods: preparative, analytical, measuring and calculating. Zootechnical evaluation of young sheep’s diet with different levels of lysine and methionine with cysteine has been conducted, and influence of these components on growth, development, metabolism, the use of amino acids and wool productivity have been studied. The importance of studying the content of amino acids in plasma is evident because they are the indicators of protein metabolism in the animals’ organism and represent themselves the exchange fund when used in the biochemical transformations in the process of updating the protein of the body tissues and the synthesis of animal products. Increase in young sheep’s diet of lysine and methionine can improve their productivity, reduce the cost of feed energy and improve biochemical indexes of meat, taking into account the optimization of its amino acid composition. Efficiency increase of young sheep during the process of the individual development is linked to conditions of feeding and in particular the usefulness of a protein food, which is primarily determined by sufficient intake of essential amino acids - lysine and methionine with cystine. Thus, the inclusion in the diet of growing young sheep some amount of synthetic amino acids – 6–8 grams of lysine and 3–4 g of methionine per 1 fodder unit, enhances oxidation-reduction processes, increase productivity, i.e. wool yield and other indicators. The content of free amino acids in plasma directly depends on their availability in feed. The use of synthetic amino acids (lysine, methionine and cystine) contributed to the increase of their content in a free state in the blood serum, indicating their increased demand in sheep. With the use of different doses of synthetic amino acids (lysine, methionine, cystine) in the diets of young sheep it has been found that the organization of optimum conditions of feeding, care and management of sheep in winter season help to avoid abrupt changes in the structure of the skin, to ensure the normal development of the histological structure and morphogenetic processes.)
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Kulikov, Denis, Ruzaliya Ulanova, and Valentina Kolpakova. "COMPREHENSIVE BIOTECHNOLOGICAL APPROACH TO PROCESSING OF PEA FLOUR FOR FOOD AND FODDER PURPOSES." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/06.

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Investigations were carried out to optimize the growth parameters of the symbiosis of cultures of the yeast Saccharomyces cerevisiae 121 and the fungus Geotrichum candidum 977 on whey waters formed from pea flour as a secondary product in the production of protein concentrates after precipitation of proteins at the isoelectric point. The whey remaining after protein precipitation is bioconverted at optimal parameters of crop growth (pH of the medium, amount of inoculum, temperature) with the formation of microbial plant concentrate (MPC) for feed purposes. Serum cultures assimilated stachyose, glucose, maltose, arabinose, and other pentoses. The mass fraction of protein in the concentrate was 57.90-61.68 % of DS. The composition of MPC obtained from biomass is balanced in essential amino acids with a speed of 107-226 %. The fatty acid composition is represented by 97 % fatty acids and 3 % - esters, aldehydes, ketones with the properties of fragrances, photo stabilizers, odor fixers, preservatives and other compounds. The ratio of the sum of saturated and unsaturated acids is 1:3, the content of cis-isomers is 91.1 %, trans-isomers are 5.1 %, omega-6 fatty acids are 19.73 %. The quality and safety indicators indicated that it is promising for use in the diet of animals.
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Steck, Roland, and Melissa L. Knothe Tate. "Application of Stochastic Network Models for the Study of Molecular Transport Processes in Bone." In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-59746.

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Osteocytes are the most abundant cells in bone. They are entombed in lacunae within the bone matrix, but are interconnected via their processes that run within the canaliculi with other osteocytes, as well as with the osteoblasts and bone lining cells on the bone surfaces, and thus from a cellular syncytium. However, the osteocytes are not immediately connected with the vasculature of bone, which means that the transport of nutrients and hormones to the cells and the removal of waste products from the cells, as well as transport of signaling molecules between the cells, has to occur either via the pericellular fluid spaces in the lacunocanalicular network, via the matrix micropores between the collagen fibers and the apatite crystals, or via intracellular transport mechanisms. Only recently our laboratory and other research groups have started to examine the transport pathways of different molecular size substances within bone systematically, using experimental tracer methods (e.g. [1, 7]). These experiments have unveiled the molecular sieving characteristics of bone: While small tracers with molecular weights of 300 Daltons (Da, e.g. glucose and small amino acids) are found in abundance throughout the bone matrix and the lacunocanalicular network, larger molecules (e.g. cytokines and serum derived proteins) are only transported through the pericellular spaces of the lacunocanalicular network. Furthermore, the transport of these substances through the lacunocanalicular network can be enhanced by mechanical loading of bone [1]. These findings highlight the importance of the lacunocanalicular network for the survival of the osteocytes and thereby tissue health. However, the state of the osteocyte syncytium is affected by age and bone diseases. It has been shown that the number of osteocytes in cortical bone decreases with age [6]. Furthermore, a histological study of cortical bone tissue samples from donors undergoing hip replacement surgery has shown that the morphology of the lacunocanalicular network is altered in diseased bone [2].
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Pokidova, Olesya Viktorovna, Nina Sergeevna Emel’yanova, Alexander Vasilievich Kulikov, Alexander Ivanovich Kotelnikov, and Natalia Alekseevna Sanina. "STUDY OF THE TRANSFORMATION OF NITROSYL IRON COMPLEX WITH N-ETHYLTHIOUREA LIGANDS IN MODEL BIOLOGICAL SYSTEMS." In NEW TECHNOLOGIES IN MEDICINE, BIOLOGY, PHARMACOLOGY AND ECOLOGY. Institute of information technology, 2021. http://dx.doi.org/10.47501/978-5-6044060-1-4.52.

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The process of transformation of a mononuclear cationic complex with N-ethylthiourea ligands in Tris-HCl buffer, as well as in a reaction mixture with reduced glutathione and bovine serum albumin, has been studied. It was found that in the presence of glutathione, the complex dimer-izes, while its initial ligands are replaced by glutathione. In the presence of albumin, the decay product of the complex is coordinated with amino acid residues (Cys34 and His39) to form a protein-bound complex.
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Reiners, J. N., and K. C. Swanson. "Effects of graded amounts of dietary leucine to milk-fed calves on serum amino acid concentrations." In 6th EAAP International Symposium on Energy and Protein Metabolism and Nutrition. The Netherlands: Wageningen Academic Publishers, 2019. http://dx.doi.org/10.3920/978-90-8686-891-9_55.

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Marshinskaia, О. V., E. V. Kiyaeva, S. V. Notova, T. V. Kazakova, and N. S. Gedulianova. "The Interdependency of Elemental and Amino Acid Composition of Blood Serum Among the Physically Challenged Athletes." In Proceedings of the International Conference on Health and Well-Being in Modern Society (ICHW 2019). Paris, France: Atlantis Press, 2019. http://dx.doi.org/10.2991/ichw-19.2019.43.

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Kolpakova, Valentina, Denis Kulikov, Ruzaliya Ulanova, Nikolay Lukin, and Irina Gaivoronskaya. "BIOCONVERSION OF CEREAL SERUM - A SECONDARY PRODUCT FOR PRODUCING PROTEIN CONCENTRATES FROM PEA AND CHICK PEAS." In GEOLINKS International Conference. SAIMA Consult Ltd, 2020. http://dx.doi.org/10.32008/geolinks2020/b1/v2/06.

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Studies on the bioconversion of whey water formed from chickpea and pea grains in the preparation of protein concentrates have been performed. The serum remaining after precipitation of the main part of the protein was subjected to a symbiotic transformation of Saccharomyces cerevisiae 121 and Geotrichum candidum 977 yeast cultures with the formation of protein-containing products with a mass fraction of protein (52.27-57.90% of DS) and a complementary amino acid composition. A microbial-plant concentrate was used as an additive in the feeding of Wistar laboratory rats. After 25 days of feeding, there was no negative effect on the physiological parameters and behavior of animals, which indicates the high quality of the protein product and the prospects of its inclusion in the composition of animal feed and diets.
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Morinaga, T., Y. Itagaki, A. Suzuki, H. Yasuda, and K. Higashio. "PURIFICATION AND CHARACTERIZATION OF TISSUE PLASMINOGEN ACTIVATOR PRODUCED BY IMR-90 CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644393.

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Tissue plasminogen activator ( t-PA ) produced by IMR-90 ( human embryonic fibroblast ) cells cultured in the serum-free medium ( DMEM ) containing 1% proteos^e peptone and 1.6 - 3.6mM CaCl2 was purified by the procedure consisted of ultrafiltration, immunoadsorpt ion chromatography, HPLC and lysine-Sepharose chromatography. The yield of t-PA from the culture broth was approximately 47%. The purified t-PA migrated as a single band on SDS-polyacrylamide gels. The molecular weight of the t-PA was estimated to be 66,000 by SDS-polyacrylamide gel electrophoresis and 69,000 by gel filtration method. Purified t-PA had a specific activity of 36 × 104 IU/mg protein by fiblin plate method or 54 - 56 X 104 IU/mg protein by clot lysis method using t-PA obtained from WHO as a standard. The amino acid composition of fibroblast t-PA was very similar to those of melanoma t-PA and uterine t-PA. Isoelectric point of fibroblast t-PA ranged from 5-7 to 8.2. The t-PA had twice as much affinity for fiblin as did high molecular weight urokinase ( UK ). Both t-PA and UK had optimum temperature at 41°C and optimum pH between 8.0 - 9.0. The polyclonal and monoclonal antibodies raised against t-PA quenched t-PA activity but had no effect on UK activity. The inhibitors of serine proteases, difluorophos-phate and gabexate mesilate, strongly inhibited the activities of fibroblast t-PA and UK. The nucleotide sequence analysis of the t-PA cDNA isolated from the cDNA library prepared from IMR-90 mRNA revealed the nucleotide changes at two positions in the coding region as compared to that of melanoma t-PA cDNA. Neither of the changes replaced the coded amino acid. The N-terminal amino acid of fibroblast t-PA was determined to be valine, indicatig the structural similarity of fibroblast t-PA to uterine t-PA.
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Verheijen, J. M., M. P. M. Caspers, G. A. W. de Munk, B. E. Enger-Valk, G. T. G. Chang, and P. H. Pouwels. "SITES IN TISSUE-TYPE PLASMINOGEN ACTIVATOR INVOLVED IN THE INTERACTION WITH FIBRIN, PLASMINOGEN AND LOW MOLECULAR WEIGHT LIGANDS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644613.

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Tissue-type plasminogen activator (t-PA) activates the proenzyme plasminogen to the active protease plasmin which degrades fibrin. The unique properties of t-PA, fibrin binding and stimulation of activity by fibrin make it an interesting molecule for specific thrombolysis. t-PA is thought to consist of five structural regions designated finger (F), growth factor (G), kringle 1 (Kl), kringle 2 (K2) and protease (P). Previous studies have shown that the interaction of t-PA with fibrin is mediated by the F and K2 regions.Mutated t-PA cDNA molecules were expressed in Chinese hamster ovary cells and t-PA analog proteins were purified from serum free culture media using affinity chromatography with immobilized monoclonal antibodies. Besides FGK1K2P (native t-PA) the following analogs were used GK1K2P, klK2P, K2P, P, FP and FGKlk2P (kl and k2 have partial deletions of the kringle). All the molecules comprising K2P could be stimulated in plasminogen activation activity by fibrinogen fragments comparable to normal t-PA. The activities of FP and FGKlk2P were only slightly influenced by these fragments. It was shown that the fibrin binding site in K2 was plasminogen dependent whereas that in F was not. K2 was found to contain a binding site for lysine, 6-amino-hexanoic acid but also 6-amino-hexane and thus to differ from the high affinity lysine binding sites in plasminogen.Chemical modification of lysine and arginine residues in t-PA with citraconic anhydride and cyclohexanedione respectively, revealed no involvement of these residues in interaction with lysine or analogs nor in stimulation of activity by fibrinogen fragments. Arginine modification led to inhibition of plasminogen activation activity, both in the presence and absence of fibrinogen fragments, but the amidolytic activity as measured with a tripeptide paranitroanilide was not changed. The involvement of one or more arginine residues in interaction of t-PA with plasminogen seems likely.
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Bauer, K. A., B. L. Kass, M. Bednarek, M. Kloczewiak, J. Hawiger, and R. D. Rosenberg. "DETECTION OF FACTOR X ACTIVATION IN HUMANS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643828.

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The activation of factor X by factor VII/VIIa-tissue factor or factor IXa plays a pivotal role in the hemostatic mechanism. This reaction results in the liberation of a peptide from the zymogen for which we have developed a sensitive and specific radioimmunoassay (RIA), The native peptide was purified from activated human factor X by hydroxylapatite chromatography and reverse-phase high pressure liquid chromatography (HPLC). Gel filtration experiments demonstrated that the peptide was not physically associated with the enzyme. A 15 amino acid peptide with the COOH-terminal sequence of the activation fragment was synthesized using the solid-phase method of Merrifield. Antisera were raised in rabbits to the synthetic analogue coupled to bovine serum albumin with glutaraldehyde. The antibody population obtained was used to construct a double antibody RIA and was able to measure as little as 0.02 nM of this component. The antibody reactivity toward the factor X zymogen was negligible (less than 1/36,000 that of the activation peptide on a molar basis). However because other plasma constituents contributed to a nonspecific basal signal in the RIA, we developed an extraction procedure for the native peptide utilizing perchloric acid. Plasma peptide levels in normal individuals were ∼0.1 nM, and elevations up to 0.8 nM were observed in patients with evidence of disseminated intravascular coagulation. Individuals chronically anticoagulated with coumarin derivatives had plasma levels of this peptide suppressed to ∼0.02 nM. The validity of our measurements of factor X activation in vivo is supported by the fact that the immunoreactive signal migrates on reverse-phase HPLC in a manner identical to that of the native activation peptide and can be quantitatively recovered. This assay should be useful for studying the pathophysiology of thrombotic as well as bleeding disorders in humans.
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Reports on the topic "Serum amino acids"

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Sela, Shlomo, and Michael McClelland. Investigation of a new mechanism of desiccation-stress tolerance in Salmonella. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7598155.bard.

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Low-moisture foods (LMF) are increasingly involved in foodborne illness. While bacteria cannot grow in LMF due to the low water content, pathogens such as Salmonella can still survive in dry foods and pose health risks to consumer. We recently found that Salmonella secretes a proteinaceous compound during desiccation, which we identified as OsmY, an osmotic stress response protein of 177 amino acids. To elucidate the role of OsmY in conferring tolerance against desiccation and other stresses in Salmonella entericaserovarTyphimurium (STm), our specific objectives were: (1) Characterize the involvement of OsmY in desiccation tolerance; (2) Perform structure-function analysis of OsmY; (3) Study OsmY expression under various growth- and environmental conditions of relevance to agriculture; (4) Examine the involvement of OsmY in response to other stresses of relevance to agriculture; and (5) Elucidate regulatory pathways involved in controlling osmY expression. We demonstrated that an osmY-mutant strain is impaired in both desiccation tolerance (DT) and in long-term persistence during cold storage (LTP). Genetic complementation and addition of a recombinantOsmY (rOsmY) restored the mutant survival back to that of the wild type (wt). To analyze the function of specific domains we have generated a recombinantOsmY (rOsmY) protein. A dose-response DT study showed that rOsmY has the highest protection at a concentration of 0.5 nM. This effect was protein- specific as a comparable amount of bovine serum albumin, an unrelated protein, had a three-time lower protection level. Further characterization of OsmY revealed that the protein has a surfactant activity and is involved in swarming motility. OsmY was shown to facilitate biofilm formation during dehydration but not during bacterial growth under optimal growth conditions. This finding suggests that expression and secretion of OsmY under stress conditions was potentially associated with facilitating biofilm production. OsmY contains two conserved BON domains. To better understand the role of the BON sites in OsmY-mediated dehydration tolerance, we have generated two additional rOsmY constructs, lacking either BON1 or BON2 sites. BON1-minus (but not BON2) protein has decreased dehydration tolerance compared to intact rOsmY, suggesting that BON1 is required for maximal OsmY-mediated activity. Addition of BON1-peptide at concentration below 0.4 µM did not affect STm survival. Interestingly, a toxic effect of BON1 peptide was observed in concentration as low as 0.4 µM. Higher concentrations resulted in complete abrogation of the rOsmY effect, supporting the notion that BON-mediated interaction is essential for rOsmY activity. We performed extensive analysis of RNA expression of STm undergoing desiccation after exponential and stationary growth, identifying all categories of genes that are differentially expressed during this process. We also performed massively in-parallel screening of all genes in which mutation caused changes in fitness during drying, identifying over 400 such genes, which are now undergoing confirmation. As expected OsmY is one of these genes. In conclusion, this is the first study to identify that OsmY protein secreted during dehydration contributes to desiccation tolerance in Salmonella by facilitating dehydration- mediated biofilm formation. Expression of OsmY also enhances swarming motility, apparently through its surfactant activity. The BON1 domain is required for full OsmY activity, demonstrating a potential intervention to reduce pathogen survival in food processing. Expression and fitness screens have begun to elucidate the processes of desiccation, with the potential to uncover additional specific targets for efforts to mitigate pathogen survival in desiccation.
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