Relevant bibliographies by topics / SERS assay

Academic literature on the topic 'SERS assay'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Contents

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'SERS assay.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "SERS assay"

1

Muhammad, Pir, Sumaira Hanif, Jiliang Yan, Fawad Ur Rehman, Jiefei Wang, Maqbool Khan, Roger Chung, et al. "SERS-based nanostrategy for rapid anemia diagnosis." Nanoscale 12, no. 3 (2020): 1948–57. http://dx.doi.org/10.1039/c9nr09152a.

Full text
Abstract:
A novel multimodal anemia diagnosis assay was developed using the SERS and compared with the routinely used clinical assays. The dual-target (iron ion/metalloprotein) capturing efficiency via strong organic cyanide affinity was utilized for whole blood analyses.
APA, Harvard, Vancouver, ISO, and other styles
2

Rashid, Md Abdur, Saiqa Muneer, Yahya Alhamhoom, and Nazrul Islam. "Rapid Assay for the Therapeutic Drug Monitoring of Edoxaban." Biomolecules 12, no. 4 (April 17, 2022): 590. http://dx.doi.org/10.3390/biom12040590.

Full text
Abstract:
Edoxaban is a direct oral anticoagulant (DOAC) that has been recently indicated for the treatment of pulmonary embolism (PE) in SARS-CoV-2 infections. Due to its pharmacokinetic variability and a narrow therapeutic index, the safe administration of the drug requires its therapeutic drug monitoring (TDM) in patients receiving the treatment. In this work, we present a label-free method for the TDM of edoxaban by surface enhanced Raman spectroscopy (SERS). The new method utilises the thiol chemistry of the drug to chemisorb its molecules onto a highly sensitive SERS substrate. This leads to the formation of efficient hotspots and a strong signal enhancement of the drug Raman bands, thus negating the need for a Raman reporter for its SERS quantification. The standard samples were run with a concentration range of 1.4 × 10−4 M to 10−12 M using a mobile phase comprising of methanol/acetonitrile (85:15 v/v) at 291 nm followed by the good linearity of R2 = 0.997. The lowest limit of quantification (LOQ) by the SERS method was experimentally determined to be 10−12 M, whereas LOQ for HPLC-UV was 4.5 × 10−7 M, respectively. The new method was used directly and in a simple HPLC-SERS assembly to detect the drug in aqueous solutions and in spiked human blood plasma down to 1 pM. Therefore, the SERS method has strong potential for the rapid screening of the drug at pathology labs and points of care.
APA, Harvard, Vancouver, ISO, and other styles
3

Zhang, Hao, Yu Yi, Chunhui Zhou, Guoqing Ying, Xiangdong Zhou, Chaopeng Fu, Yifeng Zhu, and Youqing Shen. "SERS detection of microRNA biomarkers for cancer diagnosis using gold-coated paramagnetic nanoparticles to capture SERS-active gold nanoparticles." RSC Advances 7, no. 83 (2017): 52782–93. http://dx.doi.org/10.1039/c7ra10918k.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Cordina, Nicole M., Wei Zhang, Nicolle H. Packer, and Yuling Wang. "Rapid and sensitive glycan targeting by lectin-SERS assay." Molecular Omics 16, no. 4 (2020): 339–44. http://dx.doi.org/10.1039/c9mo00181f.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Zhang, Hao, Chaopeng Fu, Yu Yi, Xiangdong Zhou, Chunhui Zhou, Guoqing Ying, Youqing Shen, and Yifeng Zhu. "A magnetic-based SERS approach for highly sensitive and reproducible detection of cancer-related serum microRNAs." Analytical Methods 10, no. 6 (2018): 624–33. http://dx.doi.org/10.1039/c7ay02727c.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Maneeprakorn, W., S. Bamrungsap, C. Apiwat, and N. Wiriyachaiporn. "Surface-enhanced Raman scattering based lateral flow immunochromatographic assay for sensitive influenza detection." RSC Advances 6, no. 113 (2016): 112079–85. http://dx.doi.org/10.1039/c6ra24418a.

Full text
Abstract:
A novel sensitive SERS-lateral flow immunochromatographic integration system using Raman active molecule-coated gold nanostar as reporters for influenza virus detection is reported. Qualitative and quantitative SERS signal detection can be achieved.
APA, Harvard, Vancouver, ISO, and other styles
7

Chuong, Tracy T., Alessia Pallaoro, Chelsea A. Chaves, Zhe Li, Joun Lee, Michael Eisenstein, Galen D. Stucky, Martin Moskovits, and H. Tom Soh. "Dual-reporter SERS-based biomolecular assay with reduced false-positive signals." Proceedings of the National Academy of Sciences 114, no. 34 (August 7, 2017): 9056–61. http://dx.doi.org/10.1073/pnas.1700317114.

Full text
Abstract:
We present a sensitive and quantitative protein detection assay that can efficiently distinguish between specific and nonspecific target binding. Our technique combines dual affinity reagents with surface-enhanced Raman spectroscopy (SERS) and chemometric analysis. We link one Raman reporter-tagged affinity reagent to gold nanoparticles and another to a gold film, such that protein-binding events create a “hot spot” with strong SERS spectra from both Raman reporter molecules. Any signal generated in this context is indicative of recognition by both affinity labels, whereas signals generated by nonspecific binding lack one or the other label, enabling us to efficiently distinguish true from false positives. We show that the number of hot spots per unit area of our substrate offers a quantitative measure of analyte concentration and demonstrate that this dual-label, SERS-linked aptasensor assay can sensitively and selectively detect human α-thrombin in 1% human serum with a limit of detection of 86 pM.
APA, Harvard, Vancouver, ISO, and other styles
8

Chen, Lei, Youngjo Sa, Yeonju Park, Hoon Hwang, Ho Ji, Bing Zhao, and Young Mee Jung. "Au-MPY/DTNB@SiO 2 SERS nanoprobe for immunosorbent assay." Vibrational Spectroscopy 87 (November 2016): 34–39. http://dx.doi.org/10.1016/j.vibspec.2016.09.004.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Gudun, Kristina, Zarina Elemessova, Laura Khamkhash, Ekaterina Ralchenko, and Rostislav Bukasov. "Commercial Gold Nanoparticles on Untreated Aluminum Foil: Versatile, Sensitive, and Cost-Effective SERS Substrate." Journal of Nanomaterials 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/9182025.

Full text
Abstract:
We introduce low-cost, tunable, hybrid SERS substrate of commercial gold nanoparticles on untreated aluminum foil (AuNPs@AlF). Two or three AuNP centrifugation/resuspension cycles are proven to be critical in the assay preparation. The limits of detection (LODs) for 4-nitrobenzenethiol (NBT) and crystal violet (CV) on this substrate are about 0.12 nM and 0.19 nM, respectively, while maximum analytical SERS enhancement factors (AEFs) are about 107. In comparative assays LODs for CV measured on AuNPs@Au film and AuNPs@glass are about 0.35 nM and 2 nM, respectively. The LOD for melamine detected on AuNPs@ Al foil is 27 ppb with 3 orders of magnitude for linear response range. Overall, AuNPs@AlF demonstrated competitive performance in comparison with AuNPs@ Au film substrate in SERS detection of CV, NBT, and melamine. To check the versatility of the AuNPs@AlF substrate we also detected KNO3 with LODs of 0.7 mM and SERS EF around 2 × 103, which is on the same order with SERS EF reported for this compound in the literature.
APA, Harvard, Vancouver, ISO, and other styles
10

Li, Ju-Mei, Chuan Wei, Wan-Fu Ma, Qiao An, Jia Guo, Jun Hu, and Chang-Chun Wang. "Multiplexed SERS detection of DNA targets in a sandwich-hybridization assay using SERS-encoded core–shell nanospheres." Journal of Materials Chemistry 22, no. 24 (2012): 12100. http://dx.doi.org/10.1039/c2jm30702b.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "SERS assay"

1

Ojha, Yagya Raj. "Selection and Characterization of ssDNA Aptamers for Salivary Peptide Histatin 3 and Their Application Towards Assay and Point-of-Care Biosensing." University of Toledo / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1575992671104993.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Sabatte, Gwenola. "Development of a SERRS antibody assay." Thesis, University of Strathclyde, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.486539.

Full text
Abstract:
Surf.'1ce enhanced resonance Raman scattering (SERRS) is an extremely sensitive detection technique, comparable to or, in some circumstances, better than fluorescence. Thanks to the molecularly specific vibrational signals, SERRS enables the identification of a specific label in situ in the presence of other materials or other labels. SERRS is produced when an analyte containing a chromophore is bound onto a.suitably roughened metal surface. The signals are recorded using Raman spectroscopy and the excitation frequency must match or be close to both the plasmon resonance frequency of the metal and an electronic transition of the chromophore. This thesis reports the development of an antibody assay using SERRS detection using colloidal silver particles as the substrate. An appropriate SERRS labelling chemistry was developed, by synthesising new SERRS labels using dyes, a polymer dye and SERRS active beads. They were conjugated to an antibody. SERRS of the labelled antibody is intense and gives specitic peaks which identify the label. Under the conditions used, antibodies were detected with a better sensitivity by SERRS detection (2.79 x 10-13 mol.dm-1 than by fluorescence (3.46 x 10-10 mol.dm·3 ). A sandwich assay was developed for two targets, brain natriuretic peptide and holotranscobalamin: The first target was used to assess the potential of several bioassay formats. The second target was added later to determine the potential for a multiple target assay. Optimisation of a complete SERRS assay using magnetic microparticles was carried out. Quantitative results were obtained with anyone run but there was variability between runs. A final investigation focused on trying to understand the source of the variability.
APA, Harvard, Vancouver, ISO, and other styles
3

Harper, Mhairi. "DNA diagnostic assays using Surface Enhanced Raman Scattering (SERS)." Thesis, University of Strathclyde, 2013. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=22401.

Full text
Abstract:
DNA is the prerequisite for all biological life and its discovery has revolutionised the understanding of biomolecular interactions and disease expression. This has enabled significant improvements in patient diagnosis and medical treatment to be carried out. The advancements in technology and instrumentation have continually progressed this knowledge and continue to push the boundaries of diagnostic and clinical advancements. One effective way to achieve this is through application of dye labelled DNA sequences and metallic nanoparticle suspensions. This research details an understanding of the interaction between dye labelled oligonucleotides and silver nanoparticle surfaces, which generate strong surface enhanced Raman scattering (SERS) responses through specific hybridisation events which correlate to the presence of targeted sequences. During this study, the attraction of oligonucleotides onto metal nanoparticles was shown to be driven through the DNA nucleobases. Therefore, the increased exposure of the base groups within single stranded DNA sequences generated a higher affinity for metal surfaces which in turn produced stronger SERS responses when compared to double stranded DNA. This principle was utilised within a DNA detection assay to successfully demonstrate the presence of target DNA sequences. Two novel DNA detection assays were also investigated which utilised SERS to determine the presence of sequences relating to the methicillin resistant Staphylococcus aureus (MRSA) strain. A solution based detection method was developed through coupling a TaqMan assay with SERS. This combination enabled highly specific detection of clinically relevant sequences of MRSA to be obtained with 7 fM limits of detection achievable. The multiple detection of different genomic S. aureus strains was achieved through the molecularly specific and narrow emission spectral profiles obtained. A contrasting DNA detection strategy which relies upon the hybridisation of comple mentary sequences on a solid substrate surface was shown. Silver nanoparticles were functionalised with specific DNA sequences and a variety of SERS active molecules, enabling the selective detection of target sequences from nitrocellulose membranes. This thesis has exploited SERS to enable the specific identification of DNA sequences to be achieved via utilisation of silver nanoparticles. Through SERS, an insight into the interactions of DNA and silver nanoparticles surfaces has been gained as well as enhancing the sensitivity and specificity achievable within SERS detection assays.
APA, Harvard, Vancouver, ISO, and other styles
4

Gracie, Kirsten. "Development of biologically relevant assays for the detection of disease DNA using SERS." Thesis, University of Strathclyde, 2014. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=24256.

Full text
Abstract:
DNA is the fundamental material responsible for storing the genetic coding required for the development of all living organisms. Since its discovery, there has been an intense amount of research into biorecognition events and the detection of DNA sequences coding for specific diseases. The development of the polymerase chain reaction (PCR) involved the amplification of small quantities of DNA allowing for subsequent analysis. However, fluorescence-based methods such as PCR have their limitations, for example the difficulties encountered when detecting multiple targets simultaneously. Therefore, there is a need for novel techniques that overcome these limitations associated with fluorescence-based methods. This research involves the use of SERS for the multiplex detection of target DNA, investigating the possible interactions between fluorescent dyes and DNA and SERS analysis of G-quadruplex formations. A SERS-based detection assay was designed for the simultaneous detection of three bacterial meningitis pathogens; Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae. By using SERS instead of fluorescence-based methods, multiplex detection was readily achieved and by using chemometric analysis it was the first report of pathogen quantification post-assay. To gain an understanding into interactions that can occur between fluorescent dyes (FAM and TAMRA), DNA and spermine, fluorescence and SERS studies were undertaken. Fluorescent studies gave an insight into the interactions that happen off the nanoparticle surface, while the SERS studies demonstrated the competitive interactions that occur between the nanoparticle surface and the two fluorescent dyes. These studies highlighted the consideration needed when selecting fluorescent dyes and target DNA sequences when designing a multiplex SERS assay. SERS was then applied to the detection of G-quadruplex formation. Previous reports used fluorescence-based methods, for example FID assays. Three ligands that selectively bind to and stabilise G-quadruplex DNA, previously used in fluorescence studies, were used and shown to have the ability to aggregate nanoparticles and act as Raman reporters. These ligands allowed for the design of the "on to off" SERS analysis of three G-quadruplex sequences.
APA, Harvard, Vancouver, ISO, and other styles
5

Desjardins, Marc. "Feasibility of vaccination for chancroid: Sero-immunology and virulence assay in an experimental model of infection." Thesis, University of Ottawa (Canada), 1996. http://hdl.handle.net/10393/9969.

Full text
Abstract:
Because of the well established epidemiologic and biologic interactions between human immunodeficiency virus (HIV) transmission and genital ulcer disease, measures to control chancroid could have a significant impact on the epidemic of HIV. I developed an IgG and IgM antibody serologic enzyme linked immunosorbent assay (ELISA) using pooled sera from clinically and microbiologically proven cases of chancroid as positive controls, and pooled sera from normal individuals without a history of sexually transmitted diseases as negative controls. Cross reactivity was minimized by absorption of serum samples with a sorbent prepared from three other Haemophilus species. Performance of the ELISA was enhanced in the period of early convalescence from acute primary chancroid and was not diminished in the presence of HIV coinfection, reflecting the tempo of the natural serologic response to and acute infection. I also developed an inhibition ELISA to determine antigenicity of potential vaccine candidates in human H. ducreyi infection, using lipooligosaccharide (LOS) as the test antigen. In a panel of 10 sera from cases of primary natural H. ducreyi infection reactive to H. ducreyi 35000 soluble antigen, only 4 samples were identified with reaction to H. ducreyi 35000 LOS. Inhibition ELISA confirmed that pre-adsorption with LOS substantially diminished reactivity of these sera to LOS but not to soluble antigen. Using the ELISA to detect immunogenicity by measuring the serologic response to vaccine candidates in rabbits, we further tested the feasibility of three bacterial antigen preparations to induce protective immunity against infection and disease. LOS carbohydrate and a pilus preparation were purified from H. ducreyi 35000 and used in a booster immunization procedure. Virulence was assayed by intra-epithelial challenge and measurement of disease for homologous strain 35000 or the virulent clinical isolate RO-34. LOS and the pilus preparation both induced humoral responses to the corresponding antigen, but the carbohydrate preparation did not. Immunization with LOS or carbohydrate did not modify virulence of infection with H. ducreyi 35000. Immunization with the strain 35000 pilus preparation significantly reduced the severity of disease, and the duration of infection and disease compared with controls. I conducted passive immunization experiments, and characterized the inflammatory infiltrate of chancroidal lesions. Naive rabbits were passively immunized with 24 or 48 mg of purified polyclonal IgG intravenously and 24 hours after infusion, challenged with the homologous strain 35000. No significant difference in disease resulting from infection with the homologous strain was observed. I then comparatively evaluated the serial immunohistology of lesions produced by infectious challenge with the homologous strain in sham-immunized or rabbits immunized with the pilus preparation. Flow cytometric analysis of rabbit peripheral blood leucocytes with CD5 and CD4 rabbit lymphocyte markers prior to infectious challenge revealed two T lymphocyte populations: CD5+CD4+ and CD5+CD4$-$. Pilus preparation vaccinee lesions showed significant quantitative acceleration and increase in T lymphocyte infiltration, and similar early increased recruitment of the CD4+ T lymphocyte subset preceding early lesion sterilization and early healing without ulceration. (Abstract shortened by UMI.)
APA, Harvard, Vancouver, ISO, and other styles
6

Phillips, Mallory Elizabeth. "Epitope mapping of African swine fever virus p72 capsid protein using polyclonal swine sera and monoclonal antibodies." Thesis, Kansas State University, 2016. http://hdl.handle.net/2097/34528.

Full text
Abstract:
Master of Science
Department of Diagnostic Medicine/Pathobiology
Raymond R. R. Rowland
African swine fever is a hemorrhagic disease of domestic pigs caused by African swine fever virus (ASFV), a double-stranded DNA virus and the only member of the family Asfarviridae. The structure of this multilayer virion contains more than 34 proteins including the protein p72 which is the major capsid protein. A single conformational neutralizing epitope has been identified on p72, but information on the other antigenic regions (epitopes) is lacking. The objective of this study was to identify p72 epitopes using polyclonal swine sera and a panel of monoclonal antibodies with the ultimate goal being the development of a blocking ELISA assay for the detection of anti-ASFV antibodies. The segment of the p72 protein from amino acids 1 to 345 was divided into five overlapping fragments which were then commercially synthesized. These fragments were cloned into the pHUE expression vector and transformed into Escherichia coli competent cells. The recombinant proteins were expressed in vitro, purified, and used as antigens in indirect ELISAs and western blots to test monoclonal antibodies and polyclonal swine sera. The monoclonal antibodies were produced against the p72 protein based on the ASFV Georgia/07 strain. The polyclonal sera were obtained from pigs immunized with a defective alphavirus replicon particle, RP-sHA-p72, expressing a recombinant protein composed of the extracellular domain of the ASFV HA protein together with the whole p72 protein. The polyclonal sera reacted to p72 in two distinct regions: between amino acids 1 and 83 and between amino acids 250 and 280. The anti-p72 reactive monoclonal antibodies reacted with p72 in three regions: between amino acids 100 and 171, amino acids 180 and 250, and amino acids 280 and 345. Fine mapping with oligopeptides allowed for the identification of six different linear epitopes. Among the monoclonal antibodies selected for blocking assay development, two have been shown to be promising candidates for further evaluation using sera from ASFV-infected pigs.
APA, Harvard, Vancouver, ISO, and other styles
7

Paiva, Lisane Mari?dne Melo de. "O sert?o (r)existe: a peleja entre o real e a inven??o na poesia de Patativa do Assar?" PROGRAMA DE P?S-GRADUA??O EM ESTUDOS DA LINGUAGEM, 2016. https://repositorio.ufrn.br/jspui/handle/123456789/21861.

Full text
Abstract:
Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-02-02T11:39:11Z No. of bitstreams: 1 LisaneMariadneMeloDePaiva_DISSERT.pdf: 939416 bytes, checksum: 8e798800e6a3cc4bbf5ca01661e0fa5c (MD5)
Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-02-03T20:26:36Z (GMT) No. of bitstreams: 1 LisaneMariadneMeloDePaiva_DISSERT.pdf: 939416 bytes, checksum: 8e798800e6a3cc4bbf5ca01661e0fa5c (MD5)
Made available in DSpace on 2017-02-03T20:26:36Z (GMT). No. of bitstreams: 1 LisaneMariadneMeloDePaiva_DISSERT.pdf: 939416 bytes, checksum: 8e798800e6a3cc4bbf5ca01661e0fa5c (MD5) Previous issue date: 2016-02-29
As poesias de Patativa do Assar? publicadas em seu primeiro livro, Inspira??o Nordestina, inspiram o desenvolvimento desta pesquisa, tendo em vista que por meio delas o estudo prop?e leituras acerca da complexidade da representa??o do Sert?o no discurso do poeta. A an?lise ? norteada pela observa??o de que tal espa?o foi cantado por Patativa com profunda fidedignidade ao seu olhar sobre ele, sem necessariamente possibilitar a constru??o de retratos similares ao que ? considerado como ?real?, ou ao que foi cristalizado pelo discurso hegem?nico. O estudo, ainda nesse sentido, interpreta as diversas tens?es sobre o signo Sert?o expostas na lira patativana, sugerindo-as como elementos constituintes de uma po?tica em estado de fronteira. Para tanto, a pesquisa apoiou-se, especialmente, nas delimita??es de Silviano Santiago (2000) sobre o conceito de entre-lugar, nos estudos sobre a tradi??o desenvolvidos por Oct?vio Paz (1984) e Amadou Hampat? B? (1982), bem como nos olhares sobre campo e cidade / sert?o e metr?poles de Raymond Williams (1989) e Durval Muniz (2011). Adentramos o discurso de Patativa partindo da compreens?o da sua constru??o enquanto sujeito, embrenhando-nos no Sert?o cantado por ele como tamb?m naquele que entra em di?logo com o dito pelo outro e, por fim, equilibramo-nos no meio da peleja desse Sert?o que existe e resiste.
Patativa do Assar??s poems published in his first book, Inspira??o Nordestina, are the inspiration to development of this study, owing to them our study showed the complexity of the representation of Sert?o in Patativa?s discourse. The analysis is based on observation that the region was sung for Patativa with deep fidelity to his view about it, without necessarily help the building of similar images to what means ?real? or to what is known by the hegemonic discourse. This study, in this sense, interpret various tensions about the sign of Sert?o shown in Patativa?s poetry, suggest them like elements which constitute a poetic in frontier condition. For that, the research, especially, is based on the theories of Silviano Santiago (2000) about the concept of ?entre-lugar?, on studies about the traditions by Oct?vio Paz (1984) and Amadou Hampat? B? (1982), as well as on researches about country and city / sert?o and metropolis by Raymond Williams (1989) and Durval Muniz (2011). The discourse of Patativa was analysed starting from the idea about his subject construction, entering in the Sert?o which was sing by him and in that one which dialogues with what is said by the other, lastly, the study is balanced in the middle of the fight of this Sert?o which exists and resists.
APA, Harvard, Vancouver, ISO, and other styles
8

Wang, Xiaodong. "Design, Syntheses, and Bioactivities of Conformationally Locked Pin1 Ground State Inhibitors." Diss., Virginia Tech, 2005. http://hdl.handle.net/10919/26625.

Full text
Abstract:
Pin1 (protein interacting with NIMA 1) is a peptidyl-prolyl isomerase involved in mitosis. As a potential anti-cancer drug target, Pin1 interacts and regulates the activity of an increasing number of cell cycle enzymes by an unknown mechanism. These cell cycle enzymes include Cdc25, Cdc27, Cyclin D1, Myt1, Wee1, NIMA, Cdc2, Plk1 and c-Myc. Recent research has revealed that Pin1 is overexpressed in a variety of cancer cell lines and Pin1 inhibitors inhibit proliferation activity of several cancer cells overexpressing Pin1. The most potent Pin1 inhibitors identified so far are in the micromolar range and no pharmacophore has been identified. In order to assist the understanding of the biological function of Pin1 using molecular probes, two amide isosteres of Ser-trans-Pro and Ser-cis-Pro dipeptides were designed and stereoselectively synthesized. The conformationally locked Ser–trans–Pro mimic, Boc-SerΨ[(E)CH=C]Pro–OH, was synthesized through the use of an Ireland-Claisen [3,3]-sigmatropic rearrangement in nine steps with 13% overall yield from a serine derivative. The Ser-cis-Pro mimic, Boc-SerΨ[(Z)CH=C]Pro–OH, was synthesized through the use of a Still-Wittig [2,3]-sigmatropic rearrangement in 11 steps with an overall yield of 20% from the same starting material. Conformationally locked peptidomimetics, including two exactly matched peptidomimetics, Ac–Phe–Phe–pSer–Ψ(E)CH=C]Pro–Arg–NH2 and Ac–Phe–Phe–pSer–Ψ[(Z)CH=C]Pro–Arg–NH2, were synthesized from these Ser-Pro isosteres using Fmoc SPPS. A protocol for in vitro Pin1 inhibition assay was established for measuring the inhibition constant for these peptidomimetics. A conformationally locked cis peptidomimetic inhibits Pin1 with a Ki of 1.7 μM, 23-fold more potent than its trans counterpart, illustrating the preference of Pin1 for a cis amide bond in its PPIase domain. The A2780 ovarian cancer cell antiproliferation activity of these peptidomimetics parallels their respective Pin1 inhibition data. This research provides a start toward more drug-like Pin1 inhibitor design. Gly–trans–Pro isosteres were synthesized using the Ireland-Claisen route. The construction of a non-peptidic (Z)-alkene library for Pin1 inhibition was attempted using the Ser-cis-Pro mimic, Boc—SerΨ[(Z)CH=C]Pro–OH as the core.
Ph. D.
APA, Harvard, Vancouver, ISO, and other styles
9

Costa, Cássia Cinara da. "Avaliação do dano e do reparo de DNA antes e depois do teste da caminhada dos seis minutos em pacientes portadores de doenças pulmonar obstrutiva crônica (DPOC)." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2008. http://hdl.handle.net/10183/14714.

Full text
Abstract:
A doença pulmonar obstrutiva crônica (DPOC) é uma doença inflamatória com participação de macrófagos, neutrófilos e linfócitos CD8, associada a estímulos oxidantes diretamente nas estruturas pulmonares, causada mais comumente pelo tabagismo. Recentemente foi demonstrado que o teste de caminhada dos seis minutos (TC6) é capaz de aumentar a inflamação e gerar o aumento de espécies reativas de oxigênio. O presente estudo teve como objetivo avaliar os níveis de dano e reparo de DNA em portadores de DPOC, quando submetidos ao TC6. Para avaliar o dano de DNA, amostras de sangue periférico foram coletadas antes e imediatamente após o TC6. Para avaliar a capacidade de reparo, foi analisada uma amostra coletada 48 horas após o TC6. Todas as amostras foram processadas pela técnica do cometa. Vinte e sete pacientes portadores de DPOC foram avaliados, sendo 59% homens. A média de idade foi de 64,7 ± 8,4 anos. A média do VEF1 foi de 40,3% ± 18,4% do previsto, com relação média de VEF1/CVF de 52,5% ± 11,9%. Todos fumaram em média 36,7 ± 17,0 anos, uma média de 48,1 ± 37,5 maços/ano. As médias das variáveis obtidas no início e no final do TC6 foram: SpO2 (92,0 ± 4,5 vs. 91,4 ± 4,6), percepção da dispnéia pela escala de BORG (1,2 ± 1,0 vs. 2,4 ± 1,7) e a distância percorrida foi em média de 380,1± 84,4 m. As taxas médias de dano de DNA antes do TC6 (27,9 ± 19,2) e imediatamente após (29,6±29,6) não apresentaram diferenças significativas (p=0,904). A análise realizada 48 horas após o TC6 demonstrou uma redução não significativa deste dano (18,3 ± 13,0, p=0,099). Conclui-se neste estudo que o esforço físico realizado durante o TC6 não provoca aumento imediato nas taxas de dano de DNA, nem estimula os mecanismos de reparo do DNA em portadores de DPOC.
Chronic obstructive pulmonary disease is an inflammatory disease in which macrophages, neutrophils and CD8 T lymphocytes play an important role. It is associated with direct oxidant stimuli of lung structures, which are most frequently triggered by smoking. A recent study showed that the 6-minute walk test (6MWT) increases inflammation and reactive oxygen species. This study evaluated DNA damage and repair in patients with COPD that took the 6MWT. To evaluate DNA damage, peripheral blood samples were collected before and immediately after 6MWT. To evaluate repair, a sample was collected 48 hours after the 6MWT. All samples were prepared for the comet assay. Twenty-seven patients with COPD were evaluated; 59% were men and mean age was 64.7 ± 8.4. Mean FEV1 was 40.3% ± 18.4% of predicted value, and mean FEV1/FVC was 52.5% ± 11.9%. Mean values before and after 6MWT were: SpO2 = 92.0 ± 4.5 vs. 91.4 ± 4.6; Borg score for dyspnea = 1.2 ± 1.0 vs. 2.4 ± 1.7; and mean distance walked – 380.1 ± 84.4 m. Mean DNA damage values before (27.9 ± 19.2) and immediately after (29.6 ± 29.6) 6MWT were not significantly different (p = 0.904). The analysis performed 48 hours after 6MWT showed a nonsignificant reduction of damage (18.3 ± 13.0; p = 0.099). Conclusions The physical effort during 6MWT did not cause an immediate increase in DNA damage, and did not stimulate DNA repair mechanisms in patients with COPD.
APA, Harvard, Vancouver, ISO, and other styles
10

Zhao, Song. "Part 1 Design, Synthesis and Bioactivity of a Phosphorylated Prodrug for the Inhibition of Pin1; Part 2 Conformational Specificity of Cdc25c Substrate for Cdc2 Kinase using LC-MS/MS." Diss., Virginia Tech, 2007. http://hdl.handle.net/10919/25989.

Full text
Abstract:
The phosphorylation-dependent PPIase (peptidyl prolyl isomerase), Pin1 (Protein interacting with NIMA#1), has been found to regulate cell cycle through a simple conformational change, the cis-trans isomerization of phospho-Ser/Thr-Pro amide bonds. A variety of key cell cycle regulatory phosphoproteins, including Cdc25 phosphatase,Cdc27, p53 oncogene, c-Myc oncogene, Wee1 kinase, Myt1 kinase, and NIMA kinas, have been confirmed as substrates of Pin1. Pin1 was also observed to be overexpressed in a variety of cancer cell lines, and the inhibitors of Pin1 showed antiproliferative activities towards these cancer cells. These results implied that Pin1 might serve as a potential anti-cancer drug target. Besides, Pin1 has an important neuroprotective function and represents a potential new therapeutic agent for Alzheimerâ s disease. In order to understand the interaction between Pin1 and Cdc25c and the role of Pin1 in the mechanism for the regulation of mitosis, two amide isosteres, Ser-Ψ[(Z)CH=C]-Pro-OH and Ser-Ψ[(E)CH=C]-Pro-OH were incorporated into two peptidomimetics derived from human Cdc25c. Phosphorylation of these two peptidomimetics by the incubation with Cdc2 was studied using LC-MS/MS technique. It was found that Cdc2 kinase was conformationally specific to its Cdc25c substrate. Only the trans conformer of Cdc25c at its Ser168-Pro position can be recognized and phosphorylated by Cdc2 kinase, thereby creating the binding site for Pin1. In an effort to improve the cell permeability of the charged inhibitors of Pin1, bisPOM (pivaloyloxymethyl) prodrug moiety was introduced to mask the phosphate group of Fmoc-pSer-Ψ[(Z)CH=C]-Pro-(2)-N-(3)-ethylaminoindole, which is one inhibitor of Pin1. Fmoc-pSer-Ψ[(Z)CH=C]-Pro-(2)-N-(3)-ethylaminoindole and its bisPOM prodrug were synthesized efficiently starting with Boc-Ser-Ψ[(Z)CH=C]-Pro-OH in 24% and 12% yields respectively. The charged inhibitor showed a moderate inhibition towards Pin1 (IC50 = 28.3 μM). Its antiproliferative activity towards A2780 ovarian cancer cells (IC50 = 46.2 μM) was significantly improved by its bisPOM prodrug (IC50 = 26.9 μM), which is comparable to the IC50 of the charged inhibitor towards Pin1 enzymatic activity. These results not only established the bisPOM strategy as an efficient prodrug choice for Pin1 inhibitors, but also added additional evidence for Pin1 as a potential anticancer drug target.
Ph. D.
APA, Harvard, Vancouver, ISO, and other styles

Books on the topic "SERS assay"

1

Barton, Richard. Serology of fungal disease. Edited by Christopher C. Kibbler, Richard Barton, Neil A. R. Gow, Susan Howell, Donna M. MacCallum, and Rohini J. Manuel. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780198755388.003.0042.

Full text
Abstract:
Examination of serum and other body fluids for the presence of antibodies to fungi, or the direct detection of the fungal antigens themselves, can play an important role in the diagnosis of fungal disease. Various methods have been applied, though currently the most commonly used is some form of enzyme-linked immunosorbent assay. Antigen detection has become a standard method for diagnosing cryptococcosis and can play a key role in detecting aspergillosis, and to a lesser extent candidiasis, depending on the underlying disease. Antibody testing is routine for many fungal diseases, including coccidioidomycosis, histoplasmosis, and many forms of aspergillosis. Beta-D-glucan is a generic fungal antigen found in the cell walls of many fungi, and detection of BDG is a test which many find useful when screening the sera of at-risk patients. Increasingly, physicians and scientists are looking to serodiagnostic tests not only to diagnose, but also to monitor treatment outcomes.
APA, Harvard, Vancouver, ISO, and other styles
2

Misra, Udayon. Unresolved Issues of Partition Politics. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780199478361.003.0005.

Full text
Abstract:
The concluding chapter takes up what it sees to be some of the major unresolved issues of Partition politics. While it tries to trace the roots of the violence centred around land in several areas of Assam, especially in the Bodo-inhabited region, it shows how issues such as the controversy over the cut-off year for immigrants to acquire citizenship are carry-overs from Partition days. Other major issues that are discussed include the status of Hindu refugees/displaced persons in the state, the National Register of Citizens, and the larger question of language and Assamese identity. It shows how with the new wave of immigrants being assimilated into the Assamese nationality, its transformation is underway and how this transformation itself throws up new challenges and equations.
APA, Harvard, Vancouver, ISO, and other styles
3

Lima, Tatiane do Nascimento, and Rogério Rodrigues Faria. Ecótono Cerrado Pantanal: meio ambiente e história natural. Editora Amplla, 2021. http://dx.doi.org/10.51859/amplla.ecp672.1121-0.

Full text
Abstract:
O termo Bioma refere-se a uma área do espaço geográfico representada por um tipo uniforme de ambiente, dentro do qual é possível identificar características similares de macroclima, fitofisionomia, solo e altitude (WALTER, 1986). Dentro dessas áreas espécies surgiram e se desenvolveram em resposta à essas características do ambiente. Tal processo permite que por exemplo, dentro dessas áreas os vegetais apresentem aspectos, formas e processos fisiológicos característicos (CRAWLEY, 1989). Dessa maneira, a manutenção desses biomas, com suas características ambientais únicas, é de fundamental importância para a manutenção da biodiversidade e dos serviços ecossistêmicos que ali ocorrem (regulação climática, ciclo de matéria, segurança alimentar, entre outros) (PBMC/BPBES, 2018; JOLY et al., 2019). O Brasil é formado por seis grandes biomas: Amazônia, Caatinga, Cerrado, Mata Atlântica, Pampas e Pantanal (IBGE, 2019). Dentro desses ambientes são encontrados uma grande diversidade de fauna e flora e características únicas de relevo e clima. Essa variedade de biomas está relacionada a grande extensão territorial do Brasil e a sua posição geográfica. Todas essas características fazem do Brasil o maior detentor de biota continental do mundo, sendo estimado um valor entre 15% e 20% das aproximadamente 1,5 milhões de espécies descritas no planeta. Só de plantas vasculares os números mais recentes citados são de 56108 espécies, com 12400 (22%) endêmicas. Esses dados representam aproximadamente 22% do total mundial (LEWINSOHN; PRADO, 2002; SHEPHERD, 2002; HUBBELL, 2008; GIAM et al., 2010). Dentro desse contexto, os biomas Cerrado e Pantanal se integram por meio dos rios que nascem nos planaltos do Cerrado. Esses rios contribuem na formação do Pantanal, nas planícies inundáveis da bacia do Paraguai (BRASIL, 2007). No Domínio Cerrado, a dinâmica ambiental é proveniente de uma marcada sazonalidade climática com duas estações bem definidas, o período seco e o período chuvoso (ASSAD, 1994; SILVA, 2011). Essa sazonalidade climática modifica constantemente as propriedades do solo, da flora e da paisagem e a reestruturação de muitas comunidades (AMARAL et al., 2013; MALHEIROS, 2016). No Pantanal as áreas conhecidas como planícies de inundação se caracterizam pela presença de hábitats que variam de aquáticos a terrestres, de acordo com o grau de comunicação com o rio principal (PAZ; TUCCI, 2010). Os ciclos de secas e cheias são um importante fenômeno hídrico para a região, criando um sistema complexo e dinâmico (JUNK; DA SILVA, 1999; RESENDE, 2008). O Cerrado é uma das 25 áreas do mundo consideradas críticas para a conservação, devido à riqueza biológica e à alta pressão antrópica a que vem sendo submetido (MYERS et al., 2000). O Pantanal, por sua vez, é reconhecido mundialmente pela abundância de sua fauna (MITTERMEIER et al., 1990; HARRIS et al., 2005) e é considerado Reserva da Biosfera e Patrimônio Natural da Humanidade pela Unesco (BRASIL, 2018). O conhecimento dos aspectos que envolvem a fauna, a flora e as características dessas paisagens são de extrema importância para a sua conservação e preservação. As áreas de transição entre esses dois biomas, chamadas áreas de ecótono, se fazem presentes no estado do Mato Grosso do Sul. Nessa região, os biomas Cerrado e Pantanal possuem correlações quanto aos aspectos geomorfológicos e fitogeográficos (RODRIGUES et al., 2017). Na região o encontro entre o Planalto de Maracaju-Campo Grande e a Planície Pantaneira é uma área comum de elementos bióticos e abióticos entre o planalto e a planície (FILHO et al., 2009). A transição entre dois ecossistemas implica a existência de uma área com valores intermediários para diversos parâmetros ambientais (NEIFF, 2003). Por um lado, a área de transição pode gerar um aumento na biodiversidade, dado o fato dessas áreas apresentarem representantes de fauna e flora dos dois ecossistemas (VELOSO et al., 1991). Contudo, essas áreas de transição podem também representarem barreira ou área de isolamento com ecossistemas vizinhos (MALANSON, 1997). Desta forma, uma análise voltada para as áreas de ecótono entre esses dois biomas faz-se necessária, uma vez que a preservação de um depende da preservação do outro. Sobretudo para o entendimento de que essas paisagens de ecótono podem ser responsáveis pelo isolamento e amortecimento das alterações dentro dos biomas Cerrado e Pantanal. Este E-book traz estudos desenvolvidos na área de ecótono Cerrado Pantanal no município de Aquidauana (MS) e entorno. O município está localizado a 130 Km a oeste da capital Campo Grande. Aquidauana por se tratar de um município com influência dos biomas Cerrado e Pantanal, abriga uma grande biodiversidade, sendo citada pelo Ministério do Meio Ambiente (BRASIL, 2002) como área prioritária para conservação da biodiversidade. Na mesma via, o município se destaca por sua vocação turística e agropecuária, o que demanda atenção, devido ao processo de intensa ocupação e exploração antrópica dos recursos naturais. Dessa maneira, o conhecimento de suas características ambientais e dos processos ecológicos desempenhados por sua fauna e flora contribuem para sua preservação e manutenção.
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "SERS assay"

1

Eremenko, Arkadiy, Il'ya Kurochkin, and Nataliya Nechaeva. "Bioanalytical systems based on cholinesterases for detection of organophosphates." In ORGANOPHOSPHORUS NEUROTOXINS, 205–18. ru: Publishing Center RIOR, 2020. http://dx.doi.org/10.29039/32_205-218.

Full text
Abstract:
Various types of electrochemical sensors based on the inhibition of butyrylcholinesterase (BChE) have been presented for the analysis of organophosphates (OPC). A special design of thick film sensors and electrochemical detector for cholinesterases assay and their inhibitors in aqueous samples has been developed. For this assay, thiol sensitive sensors based on screen printed graphite electrode modified with nanoparticles of manganese dioxide were used. High sensitivity of manganese dioxide modified thick film sensors towards thiocholine and therefore low detection limit of BChE (1 pM) enabled their use for subnanomolar detection of an organophosphate pesticide diazinon, and other irreversible inhibitors of BChE. This work also presents modern innovative approach for the analysis of BChE by Raman spectroscopy. New SERS-substrates based on silver paste for sensitive quantification of BChE activity were obtained, characterized and applied to thiocholine detection, with LOD (TCh) being 260 nM. Real samples of human plasma were analyzed; a good correlation between spectrophotometric detection and Raman detection was shown. The developed technique is inexpensive and easy-to-use and has promising potential for analysis of OPC.
APA, Harvard, Vancouver, ISO, and other styles
2

Eremenko, Arkadiy, Il'ya Kurochkin, and Nataliya Nechaeva. "Bioanalytical systems based on cholinesterases for detection of organophosphates." In Organophosphorous Neurotoxins, 0. ru: Publishing Center RIOR, 2020. http://dx.doi.org/10.29039/chapter_5e4132b6096d14.18045940.

Full text
Abstract:
Various types of electrochemical sensors based on the inhibition of butyrylcholinesterase (BChE) have been presented for the analysis of organophosphates (OPC). A special design of thick film sensors and electrochemical detector for cholinesterases assay and their inhibitors in aqueous samples has been developed. For this assay, thiol sensitive sensors based on screen printed graphite electrode modified with nanoparticles of manganese dioxide were used. High sensitivity of manganese dioxide modified thick film sensors towards thiocholine and therefore low detection limit of BChE (1 pM) enabled their use for subnanomolar detection of an organophosphate pesticide diazinon, and other irreversible inhibitors of BChE. This work also presents modern innovative approach for the analysis of BChE by Raman spectroscopy. New SERS-substrates based on silver paste for sensitive quantification of BChE activity were obtained, characterized and applied to thiocholine detection, with LOD (TCh) being 260 nM. Real samples of human plasma were analyzed; a good correlation between spectrophotometric detection and Raman detection was shown. The developed technique is inexpensive and easy-to-use and has promising potential for analysis of OPC.
APA, Harvard, Vancouver, ISO, and other styles
3

Cottrez, Françoise, Elodie Boitel, Claude Auriault, and Hervé Groux. "The SENS-IS Assay." In Alternatives for Dermal Toxicity Testing, 361–75. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-50353-0_25.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Kitikoon, Pravina, Phillip C. Gauger, and Amy L. Vincent. "Hemagglutinin Inhibition Assay with Swine Sera." In Methods in Molecular Biology, 295–301. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0758-8_24.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Hu, Xiaoxia, and Quan Yuan. "Sandwich Assays Based on QCM, SPR, Microcantilever, and SERS Techniques for Nucleic Acid Detection." In Biosensors Based on Sandwich Assays, 149–65. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-7835-4_9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Zhan, Shenshan, Xiaoding Lou, Pei Zhou, and Fan Xia. "Sandwich Assays Based on SPR, SERS, GMR, QCM, Microcantilever, SAW, and RRS Techniques for Protein Detection." In Biosensors Based on Sandwich Assays, 69–91. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-7835-4_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Zauli, D., C. Crespi, F. B. Bianchi, M. Musiani, and P. Tazzari. "Immunofluorescent Methods for the Assay of Cytoskeleton Antibodies in Human Sera." In Reviews on Immunoassay Technology, 165–76. London: Palgrave Macmillan UK, 1988. http://dx.doi.org/10.1007/978-1-349-09854-5_8.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Kinders, R., J. Slota, J. Patrick, C. Plate, I. Kafer, W. Caminiti, H. Rittenhouse, and G. Manderino. "An Assay for Cryptic Tumor Antigens in Sera of Women with Breast Cancer." In Breast Cancer Immunodiagnosis and Immunotherapy, 55–67. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4757-1296-4_6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Algaissi, Abdullah, and Anwar M. Hashem. "Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay." In Methods in Molecular Biology, 107–16. New York, NY: Springer US, 2019. http://dx.doi.org/10.1007/978-1-0716-0211-9_9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Pape, P. Le, and J. Deunff. "Detection of Aspergillus Glycoprotein in Sera of Patients with Invasive Aspergillosis by an Enzyme-Linked Immunosorbent Assay." In Fungal Antigens, 382. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-0773-0_56.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "SERS assay"

1

Baritaux, Jean-Charles, Antoine Hoang, and Nathalie Morel. "Automated readout of a SERS lateral flow assay." In Preclinical and Clinical Optical Diagnostics, edited by J. Quincy Brown and Ton G. van Leeuwen. SPIE, 2019. http://dx.doi.org/10.1117/12.2527039.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Yu, Wei W., and Ian M. White. "Optofluidic SERS on Paper: A Lateral Flow Concentration Assay Using Inkjet Fabricated SERS-Active Substrates." In CLEO: Science and Innovations. Washington, D.C.: OSA, 2012. http://dx.doi.org/10.1364/cleo_si.2012.cth4l.1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Mu, Zhongde, Xiangwei Zhao, and Zhongze Gu. "Multiplex and Label-free SERS Protein Assay Based on Colloidal Photonic Crystal Beads." In Biomedical Optics. Washington, D.C.: OSA, 2014. http://dx.doi.org/10.1364/biomed.2014.bs3a.46.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Yu, Qian, Xianming Kong, Yibo Ma, and Alan X. Wang. "Multi-functional cellulose fiber decorated with plasmonic Au nanoparticles for colorimetry and SERS assay." In CLEO: Applications and Technology. Washington, D.C.: OSA, 2018. http://dx.doi.org/10.1364/cleo_at.2018.jw2a.180.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Shende, Chetan, Atanu Sengupta, Hermes Huang, and Stuart Farquharson. "Detection of pathogens in food using a SERS-based assay in just a few hours." In SPIE Sensing Technology + Applications, edited by Moon S. Kim and Kuanglin Chao. SPIE, 2014. http://dx.doi.org/10.1117/12.2054286.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Perumal, Jayakumar, Ran Zhi Tong Chua, Mohesh Moothanchery, Poongkulali Rajarahm, Aniza Puteri Mahyuddin, Ghayathri Balasundaram, Mahesh Choolani, and Malini Olivo. "Rapid and sensitive detection of disease biomarkers using SERS assay in a simplified Raman POC device." In Optical Diagnostics and Sensing XXIII: Toward Point-of-Care Diagnostics, edited by Gerard L. Coté. SPIE, 2023. http://dx.doi.org/10.1117/12.2664254.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Schechinger, Monika, Haley Marks, Andrea Locke, Mahua Choudhury, and Gerard Coté. "Optimization of surface enhanced Raman scattering (SERS) assay for the transition from benchtop to handheld Raman systems." In SPIE BiOS, edited by Gerard L. Coté. SPIE, 2017. http://dx.doi.org/10.1117/12.2255949.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Locke, Andrea K., Gerard L. Coté, and Nicolaas E. P. Deutz. "Development of a paper-based vertical flow SERS assay for citrulline detection using aptamer-conjugated gold nanoparticles." In Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics, edited by Gerard L. Coté. SPIE, 2018. http://dx.doi.org/10.1117/12.2290604.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Bhardwaj, Vinay, Supriya Srinivasan, and Anthony J. McGoron. "On-chip surface-enhanced Raman spectroscopy (SERS)-linked immuno-sensor assay (SLISA) for rapid environmental-surveillance of chemical toxins." In SPIE Sensing Technology + Applications, edited by Tuan Vo-Dinh, Robert A. Lieberman, and Günter G. Gauglitz. SPIE, 2015. http://dx.doi.org/10.1117/12.2182591.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Han, Sungyub, Luke A. Oaks, Andrea K. Locke, Yi-Shing Lisa Cheng, and Gerard L. Coté. "Development of a free-solution SERS-based assay for point-of-care oral cancer biomarker detection using DNA-conjugated gold nanoparticles." In Optical Diagnostics and Sensing XVIII: Toward Point-of-Care Diagnostics, edited by Gerard L. Coté. SPIE, 2018. http://dx.doi.org/10.1117/12.2290516.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "SERS assay"

1

Perk, Simon, Egbert Mundt, Alexander Panshin, Irit Davidson, Irina Shkoda, Ameera AlTori, and Maricarmen Garcia. Characterization and Control Strategies of Low Pathogenic Avian Influenza Virus H9N2. United States Department of Agriculture, November 2012. http://dx.doi.org/10.32747/2012.7697117.bard.

Full text
Abstract:
The avian influenza virus, subtype H9N2 subtype, defined as having a low pathogenicity, causes extensive economical losses in commercial flocks, probably due to management and synergism with other pathogens. AIV H9N2 was first identified in Israel in the year 2000, and since then it became endemic and widespread in Israel. Control by vaccination of commercial flocks with an inactivated vaccine has been introduced since 2007. In face of the continuous H9N2 outbreaks, and the application of the vaccination policy, we aimed in the present study to provide a method of differentiating naturally infected from vaccinated animals (DIVA). The aim of the assay would be detect only antibodies created by a de-novo infection, since the inactivated vaccine virus is not reproducing, and might provide a simple tool for mass detection of novel infections of commercial flocks. To fulfill the overall aim, the project was designed to include four operational objectives: 1. Evaluation of the genetic evolution of AIV in Israel; 2. Assessment of the diagnostic value of an NS1 ELISA; 3. NS1 ELISA as evaluation criteria for measuring the efficacy of vaccination against H9N2 AIV; 4. Development of an AIV H9 subtype specific ELISA systems. Major conclusion and implications drawn from the project were: 1. A continuous genetic change occurred in the collection of H9N2 isolates, and new introductions were identified. It was shown thatthe differences between the HA proteins of viruses used for vaccine productionand local fieldisolatesincreasedin parallelwith the durationand intensity ofvaccine use, therefore, developing a differential assay for the vaccine and the wild type viruses was the project main aim. 2. To assess the diagnostic value of an NS1 ELISA we first performed experimental infection trials using representative viruses of all introductions, and used the sera and recombinant NS1 antigens of the same viruses in homologous and heterologous NS1 ELISA combination. The NS1 ELISA was evidently reactive in all combinations, and did not discriminate significantly between different groups. 3. However, several major drawbacks of the NS1 ELISA were recognized: a) The evaluation of the vaccination effect in challenged birds, showed that the level of the NS1 antibodies dropped due to the vaccination-dependent virus level drop; b) the applicability of the NS1-ELISA was verified on sera of commercial flocks and found to be unusable due to physico-chemical composition of the sera and the recombinant antigen, c) commercial sera showed non-reactivity that might be caused by many factors, including vaccination, uncertainty regarding the infection time, and possibly low antigen avidity, d) NS1 elevated antibody levels for less than 2 months in SPF chicks. Due to the above mentioned reasons we do not recommend the application of the DIVA NS1 ELISA assay for monitoring and differentiation AIV H9N2 naturally-infected from vaccinated commercial birds.
APA, Harvard, Vancouver, ISO, and other styles
2

Anderson, Olin, and Gad Galili. Development of Assay Systems for Bioengineering Proteins that Affect Dough Quality and Wheat Utilization. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568781.bard.

Full text
Abstract:
The quality and utilization of wheat is largely dependent upon the exact physical/chemical properties of the doughs made from flour/water mixtures. Among the wheat seed components most correlated with dough visoelastic parameters are the high-molecular-weight (HMW) glutenin subunits whose disulfide cross-linked macropolymer is critical for dough functionality. We have used the tools of molecular biology, wheat transformation, heterologous expression of HMW-glutenin subunits in bacteria, and dough micro-mixing experiments to examine some of the molecular basis of HMW-glutenin functionality. In addition, we have developed sets of modified and synthetic gene constructs and transgenic wheat lines that will allow further examination of the role of the HMW-glutenins. Among the results from this work is evidence that the HMW-glutenin repeat domain is directly related to dough properties, the demonstration that interaction between subunits is dependent upon domain presence but not order, a novel understanding of the restrictions on intra-vs inter-chain disulfide bonds, the demonstration that HMW-glutenin genes can be transformed into wheat for simultaneously high expression of the transgene and suppression of the endogenous genes, and the construction of a set of modified HMW-glutenins capable of being epitope tagged for studying polypeptide subcellular processing and the fate of HMW-glutenins in dough mixing experiments.
APA, Harvard, Vancouver, ISO, and other styles
3

Heifetz, Yael, and Michael Bender. Success and failure in insect fertilization and reproduction - the role of the female accessory glands. United States Department of Agriculture, December 2006. http://dx.doi.org/10.32747/2006.7695586.bard.

Full text
Abstract:
The research problem. Understanding of insect reproduction has been critical to the design of insect pest control strategies including disruptions of mate-finding, courtship and sperm transfer by male insects. It is well known that males transfer proteins to females during mating that profoundly affect female reproductive physiology, but little is known about the molecular basis of female mating response and no attempts have yet been made to interfere with female post-mating responses that directly bear on the efficacy of fertilization. The female reproductive tract provides a crucial environment for the events of fertilization yet thus far those events and the role of the female tract in influencing them are poorly understood. For this project, we have chosen to focus on the lower reproductive tract because it is the site of two processes critical to reproduction: sperm management (storage, maintenance, and release from storage) and fertilization. E,fforts during this project period centered on the elucidation of mating responses in the female lower reproductive tract The central goals of this project were: 1. To identify mating-responsive genes in the female lower reproductive tract using DNA microarray technology. 2. In parallel, to identify mating-responsive genes in these tissues using proteomic assays (2D gels and LC-MS/MS techniques). 3. To integrate proteomic and genomic analyses of reproductive tract gene expression to identify significant genes for functional analysis. Our main achievements were: 1. Identification of mating-responsive genes in the female lower reproductive tract. We identified 539 mating-responsive genes using genomic and proteomic approaches. This analysis revealed a shift from gene silencing to gene activation soon after mating and a peak in differential gene expression at 6 hours post-mating. In addition, comparison of the two datasets revealed an expression pattern consistent with the model that important reproductive proteins are pre-programmed for synthesis prior to mating. This work was published in Mack et al. (2006). Validation experiments using real-time PCR techniques suggest that microarray assays provide a conservativestimate of the true transcriptional activity in reproductive tissues. 2.lntegration of proteomics and genomics data sets. We compared the expression profiles from DNA microarray data with the proteins identified in our proteomic experiments. Although comparing the two data sets poses analyical challenges, it provides a more complete view of gene expression as well as insights into how specific genes may be regulated. This work was published in Mack et al. (2006). 3. Development of primary reproductive tract cell cultures. We developed primary cell cultures of dispersed reproductive tract cell types and determined conditions for organ culture of the entire reproductive tract. This work will allow us to rapidly screen mating-responsive genes for a variety of reproductive-tract specifi c functions. Scientific and agricultural significance. Together, these studies have defined the genetic response to mating in a part of the female reproductive tract that is critical for successful fertllization and have identified alarge set of mating-responsive genes. This work is the first to combine both genomic and proteomic approaches in determining female mating response in these tissues and has provided important insights into insect reproductive behavior.
APA, Harvard, Vancouver, ISO, and other styles
4

Blum, Abraham, Henry T. Nguyen, and N. Y. Klueva. The Genetics of Heat Shock Proteins in Wheat in Relation to Heat Tolerance and Yield. United States Department of Agriculture, August 1993. http://dx.doi.org/10.32747/1993.7568105.bard.

Full text
Abstract:
Fifty six diverse spring wheat cultivars were evaluated for genetic variation and heritability for thermotolerance in terms of cell-membrane stability (CMS) and triphenyl tetrazolium chloride (TTC) reduction. The most divergent cultivars for thermotolerance (Danbata-tolerant and Nacozari-susceptible) were crossed to develop an F8 random onbred line (RIL) population. This population was evaluated for co-segragation in CMS, yield under heat stress and HSP accumulation. Further studies of thermotolerance in relations to HSP and the expression of heterosis for growth under heat stress were performed with F1 hybrids of wheat and their parental cultivars. CMS in 95 RILs ranged from 76.5% to 22.4% with 71.5% and 31.3% in Danbata and Nacozari, respectively. The population segregated with a normal distribution across the full range of the parental values. Yield and biomass under non-stress conditions during the normal winter season at Bet Dagan dit not differ between the two parental cultivar, but the range of segregation for these traits in 138 RILs was very high and distinctly transgressive with a CV of 35.3% and 42.4% among lines for biomass and yield, respectively. Mean biomass and yield of the population was reduced about twofold when grown under the hot summer conditions (irrigated) at Bet Dagan. Segregation for biomass and yield was decreased relative to the normal winter conditions with CV of 20.2% and 23.3% among lines for biomass and yield, respectively. However, contrary to non-stress conditions, the parental cultivars differed about twofold in biomass and yield under heat stress and the population segregated with normal distribution across the full range of this difference. CMS was highly and positively correlated across 79 RILs with biomass (r=0.62**) and yield (r=0.58**) under heat stress. No such correlation was obtained under the normal winter conditions. All RILs expressed a set of HSPs under heat shock (37oC for 2 h). No variation was detected among RILs in high molecular weight HSP isoforms and they were similar to the patterns of the parental cultivars. There was a surprisingly low variability in low molecular weight HSP isoforms. Only one low molecular weight and Nacozari-specific HSP isoform (belonging to HSP 16.9 family) appeared to segregate among all RILs, but it was not quantitatively correlated with any parameter of plant production under heat stress or with CMS in this population. It is concluded that this Danbata/Nacozari F8 RIL population co-segregated well for thermotolerance and yield under heat stress and that CMS could predict the relative productivity of lines under chronic heat stress. Regretfully this population did not express meaningful variability for HSP accumulation under heat shock and therefore no role could be seen for HSP in the heat tolerance of this population. In the study of seven F1 hybrids and their parent cultivars it was found that heterosis (superiority of the F1 over the best parent) for CMs was generally lower than that for growth under heat stress. Hybrids varied in the rate of heterosis for growth at normal (15o/25o) and at high (25o/35o) temperatures. In certain hybrids heterosis for growth significantly increased at high temperature as compared with normal temperature, suggesting temperature-dependent heterosis. Generally, under normal temperature, only limited qualitative variation was detected in the patterns of protein synthesis in four wheat hybrids and their parents. However, a singular protein (C47/5.88) was specifically expressed only in the most heterotic hybrid at normal temperature but not in its parent cultivars. Parental cultivars were significantly different in the sets of synthesized HSP at 37o. No qualitative changes in the patterns of protein expression under heat stress were correlated with heterosis. However, a quantitative increase in certain low molecular weight HSP (mainly H14/5.5 and H14.5.6, belonging to the HSP16.9 family) was positively associated with greater heterosis for growth at high temperature. None of these proteins were correlated with CMS across hybrids. These results support the concept of temperature-dependent heterosis for growth and a possible role for HSP 16.9 family in this respect. Finally, when all experiments are viewed together, it is encouraging to find that genetic variation in wheat yield under chronic heat stress is associated with and well predicted by CMS as an assay of thermotolerance. On the other hand the results for HSP are elusive. While very low genetic variation was expressed for HSP in the RIL population, a unique low molecular weight HSP (of the HSP 16.9 family) could be associated with temperature dependant heterosis for growth.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'SERS assay' for particular source types:
Journal articles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography