Dissertations / Theses on the topic 'Sequence-function'
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McDougall, Rachel Clare. "Schizosaccharomyces pombe : from sequence to function." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627369.
Full textSpencer, Sarah Jean. "Linking sequence to function in microbial genomics." Thesis, Massachusetts Institute of Technology, 2017. http://hdl.handle.net/1721.1/113437.
Full textThis electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references (pages 129-140).
Microbial genomes show high plasticity due to horizontal transfer, large community sizes, and rapid growth paired with adaptive mutations. Despite this mutability of gene content, most studies of microbial communities still rely on bulk, single-gene amplicon sequencing. In this thesis, I present methods that interrogate the gene content of single cells derived from complex natural communities. In the first project, I present a novel molecular biology method to link a bacterial functional gene to its host species with single-cell resolution. This high-throughput protocol is applied to assess the distribution of anaerobic respiration genes in a lake ecosystem. In the second project, I demonstrate extensions of this methodology to link genes between spatially proximal microbial cells, and apply this approach to probe the spatial organization of human dental plaque using DNA sequencing. In the final project, I completed whole-genome sequencing of environmental isolates derived from single, cultivable cells and employ mutational and horizontal transfer analysis to demonstrate adaptation to harsh environmental conditions in contaminated groundwater. These projects demonstrate the rich information stored within each microbial genome and the impact of spatial distribution in the environment. Each effort also contributes or highlights new molecular biology techniques to generate genomic data from individual microbial cells.
by Sarah Jean Spencer.
Ph. D.
Warnecke, Tobias. "Determinants of coding sequence evolution- beyong protein function." Thesis, University of Bath, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531341.
Full textOtto, Gary Edward. "Sequence comparison and the prediction of protein function." Thesis, Massachusetts Institute of Technology, 1986. http://hdl.handle.net/1721.1/14888.
Full textHamilton, Russell S. "DAROGAN : enzyme function prediction from multiple sequence alignments." Thesis, University of Edinburgh, 2006. http://hdl.handle.net/1842/14972.
Full textColafranceschi, Mauro. "Distribution of Hydrophobicity patterns in proteins primary structures: a statistical study." Doctoral thesis, La Sapienza, 2005. http://hdl.handle.net/11573/916823.
Full textRünger, Dennis. "On the generation and function of conscious sequence knowledge." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät II, 2008. http://dx.doi.org/10.18452/15845.
Full textThere is a general consensus that incidental learning can produce conscious knowledge about a hidden sequential regularity, even though the underlying learning mechanisms are still poorly understood. By contrast, whether sequence learning can also be “implicit” or nonconscious is a matter of intense debate. Progress can be achieved by grounding research on conscious and nonconscious learning in larger theoretical frameworks of consciousness. Rünger and Frensch (2008a) show how “conscious sequence knowledge” can be defined and operationalized in reference to global workspace theory of consciousness that depicts “inferential promiscuity” as the functional hallmark of conscious mental representations. Rünger and Frensch (2008b) test a central prediction of the unexpected-event hypothesis — a theoretical account of the generation of conscious knowledge in incidental learning situations. In a series of experiments, unexpected events were induced experimentally by disrupting the incidental learning process. In line with the unexpected-event hypothesis, the authors observed an increased availability of conscious sequence knowledge. Finally, Rünger, Nagy, and Frensch (in press) explore the function of conscious sequence knowledge in the context of a sequence recognition test. The empirical results suggest that conscious sequence knowledge provides the epistemic basis for reasoned — as opposed to intuitive or heuristic — judgments.
Kandaswamy, Krishna Kumar [Verfasser]. "Sequence function classification by machine learning methods / Krishna Kumar Kandaswamy." Lübeck : Zentrale Hochschulbibliothek Lübeck, 2012. http://d-nb.info/1023624257/34.
Full textPryde, Fiona E. "Function of subtelomeric repeat sequence in the yeast Saccharomyces cerevisiae." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302603.
Full textWang, Pam Shou-Ping. "Exploring the sequence-structure-function relationship in beta-peptide foldamers." Thesis, Yale University, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=3580893.
Full textThe interplay between sequence, structure and function is an underlying theme in biological systems. Proteins, in particular, have evolved the ability to access a virtually infinite set of three-dimensional architectures from a small collection of building blocks; it is precisely this complexity of form that finely tunes their functional specificity. β-Peptides are a class of unnatural polyamides known to adopt structural motifs that are in many ways reminiscent of protein folds in nature. This dissertation first investigates the relationship between sequence and structure in self-assembling β-peptides, then demonstrates how the latter translates into function.
Chapter 1 provides an overview of the fundamental principles guiding β-peptide helix formation and self-assembly, and describes their applications both within and outside of the biological context. The ability of β-peptides to mimic natural α-helices while maintaining proteolytic resistance allows them to serve as therapeutic agents by targeting, for example, protein-protein interactions. Their unique stability in both aqueous and organic environments further enables the development of β-peptide-based nanomaterials and organocatalysts.
Chapter 2 elucidates the relationship between β-peptide primary sequence and quaternary structure based on the biophysical characterization of the Acid-3Y bundle. Acid-3Y was designed by substituting isoleucine for leucine side-chains in the sequence of the previously characterized octamer, Acid-1Y. The finding that Acid-3Y assembles into a tetrameric bundle suggests that branching at the γ-carbon of hydrophobic residues plays a critical role in determining β-peptide bundle stoichiometry.
Chapter 3 explores the potential of β-peptide bundles to mimic enzyme structure and function. The demonstration of β-peptide mutarotase activity in benzene highlights the importance of macromolecular preorganization in catalysis, while the ability of rationally designed β-peptide bundles to catalyze ester hydrolysis in water represents a crucial step towards the functionalization of these unnatural macromolecules. The dependence of catalytic activity on both active site geometry and bundle assembly, together with their substrate selectivity, underscores the unique biomimetic capacity of β-peptides.
Chapter 4 describes the rational design of a β-peptide ligand for the parathyroid hormone 1 receptor (PTH1R). Using previous strategies that led to the identification of p53 and GLP-1 mimics, a 12-member β-peptide library was constructed and tested in vitro for binding to the receptor protein. Although no hits were found from this initial screen, subsequently designed α/β-peptide chimeras showed promise as synthetic antagonists of PTH1R with improved pharmacokinetic properties.
Chapter 5 summarizes the key results of this dissertation and offers a perspective on possible future research directions. A breakthrough in the field of β-peptides would rely on the development of a method to synthesize genuine "β-proteins" with more sophisticated structure and function.
Crowley, Louis J. "Structure-function studies of conserved sequence motifs of cytochrome b5 reductase." [Tampa, Fla] : University of South Florida, 2007. http://purl.fcla.edu/usf/dc/et/SFE0001913.
Full textHvidsten, Torgeir R. "Predicting Function of Genes and Proteins from Sequence, Structure and Expression Data." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4490.
Full textBerti, Paul J. (Paul Joseph). "Cysteine proteases : interaction with cystatin C and sequence to structure-function relationships." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41350.
Full textMukendi, Mujinga Grace. "Sequence and function-based screening of goat rumen metagenome for novel lipases." Thesis, Vaal University of Technology, 2019. http://hdl.handle.net/10352/463.
Full textLipases have been one of the important biocatalysts that catalyse the transformation of lipids to yield very important products that can be of beneficial in food, agriculture, pharmaceutical medicine and for the biodiesel production. In the search for novel biocatalysts, notably lipases, the conventional culture-based techniques were used but it can only address sourcing the biomolecule from 1-10% of the microbial population leaving the wealth of the biomolecules packed in 90-99% of the microbial community unaccounted for. Metagenomic technique, which is culture-independent, was developed as a comprehensive approach to address literally 100% of the microbial population thereby maximizing the chances of obtaining novel biocatalysts with superior physico-chemical and catalytic traits. In principle, any biomolecule including lipase could be sourced from any biologically-active environment, of which animal rumen is one. However, among the rumenant animals goat has diverse feeding habit, thereby laying ground for increased microbial diversity in its gastro-intestinal tract. It was thus, postulated that goat rumen could be potential source of novel lipase isoforms. Therefore, the aim of the study was to extract metagenomic DNA from goat rumen and construct a metagenomic fosmid library and screen the library for lipase isoforms. The fosmid clones were functionally screened using 1% tributyrin as a substrate and five positive clones were selected. From the five clones, two fosmids were selected for further study. Following nucleotide sequencing and in-silico analysis of the insert of the two selected clones, one lipase encoding open reading frame (Lip-VUT3 and Lip-VUT5) from each fosmid clones of approximately 212 and 248 amino acids, respectively, was identified. The amino acid sequences of the Lip-VUT3 ORF contained a classical conserved lipase GSDL sequence motif while the amino acid sequences of the Lip-VUT5 ORF contained a classical G-L-S-L-G conserved lipase/esterase motif sequence. The two genes (Lip-VUT3 and Lip-VUT5) were successfully expressed in Escherichia coli BL21 (DE3) and the purified enzymes exhibited respective temperature optima of 60 °C and 70 °C, and respective pH optima of 6.0 and 10.0. Further biochemical characterisation indicated that Lip-VUT3 and Lip-VUT5 lipases showed tolerance towards a wide concentration (50%-100%) of methanol. Lip-VUT3 had a Km value of 0.287 mM while Lip-VUT5 had a Km value of 0.556 Mm. This shows that Lip-VUT3 lipase has a higher affinity for olive oil than Lip-VUT5. Lip-VUT3 and Lip-VUT5 were characterised to be true lipases that have been recovered from the rumen environment through metagenomic approach. Therefore, the study proved that metagenomic approach helps in recovering novel lipase isoforms with potential down stream industrial and therapeautic applications from goat rumen metagenome, a rich but untapped source.
Wass, Mark Nicholas. "Development of a sequence based method for the prediction of protein function." Thesis, Imperial College London, 2008. http://hdl.handle.net/10044/1/11542.
Full textDi, Domenico Tomás. "Computational Analysis and Annotation of Proteome Data: Sequence, Structure, Function and Interactions." Doctoral thesis, Università degli studi di Padova, 2014. http://hdl.handle.net/11577/3423805.
Full textCon l'avvento delle tecnologie di sequenziamento moderne, la quantità di dati biologici disponibili ha cominciato a sfidare la nostra capacità di elaborarli. È diventato quindi essenziale sviluppare nuovi strumenti e tecniche capaci di produrre dei risultati basati su grandi moli di informazioni. Questa tesi si concentra sullo sviluppo di tali strumenti computazionali e dei metodi per lo studio dei dati proteici. Viene dapprima presento il lavoro svolto per la comprensione delle proteine intrinsecamente disordinate. Attraverso lo sviluppo di nuovi predittori di disordine, siamo stati in grado di sfruttare le fonti di dati attualmente disponibili per annotare qualsiasi proteina avente sequenza nota. Memorizzando queste predizioni, insieme ai dati provenienti da altre fonti, è stato creato MobiDB. Questa risorsa fornisce una visione completa sulle annotazioni di disordine disponibili per una qualsiasi proteina di interesse presente nel database UniProt. Sulla base delle osservazioni ottenute da questo strumento, è stato quindi creato un workflow di analisi dei dati con l'obiettivo di approfondire la nostra comprensione delle proteine intrinsecamente disordinate. La seconda parte della tesi si concentra sulle proteine ripetute. Il metodo RAPHAEL è stato sviluppato per contribuire nell'identificazione di strutture proteiche ripetute all'interno dei file PDB. Le strutture selezionate da questo strumento sono state poi catalogate manualmente utilizzando uno schema formale di classificazione, e pubblicate quindi come parte del database RepeatsDB. Infine, viene descritto lo sviluppo di strumenti basati su grafi per l'analisi di dati proteici. RING consente all'utente di visualizzare e studiare la struttura di una proteina come una rete di nodi collegati da tra loro da proprietà fisico-chimiche. Il secondo metodo, PANADA, consente all'utente di creare reti di similarità di proteine e di valutare la trasferibilità delle annotazioni funzionali tra cluster diversi.
Milburn, Duncan. "Computational analysis of the functional sites in proteins." Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325850.
Full textAl-Shahib, Ali Walid. "Addressing the core challenges in predicting protein function from sequence using machine learning." Thesis, University of Glasgow, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.425167.
Full textYekta, Soraya. "An evolutionary perspective on the sequence, mechanism, and regulatory function of animal microRNAs." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/43791.
Full textThesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, June 2008.
Includes bibliographical references.
(cont.) The vertebrate-specific miR-196 family is encoded at three paralogous loci in the mammalian Hox clusters, and has complementarity to messages of several Hox genes, including Hox8 paralogues. RNA fragments diagnostic of miR-196-directed cleavage of Hoxb8 were detected in mouse embryos. Cell culture experiments demonstrated down-regulation of Hoxb8, Hoxc8, Hoxd8, and Hoxa7 and supported a cleavage mechanism for miR-196-directed repression of Hoxb8. These results point to a miRNA-mediated mechanism for the posttranscriptional restriction of Hox gene expression during vertebrate development and demonstrate that metazoan miRNAs can repress expression of their natural targets through mRNA cleavage in addition to inhibiting a translational step. Inhibition of the two Hox miRNAs in chick embryos, resulted in axial skeletal patterning defects in domains that overlap considerably with Hox target and miRNA expression, supporting a specialization of miRNAs in Hox gene regulation, and consistent with action by miR-196 and miR-10 to refine posterior boundaries at relative levels of expression for multiple Hox genes. The genomic distribution of target sites and Hox patterns of expression suggest that the miRNAs further act in concert with more posteriorly expressed Hox genes to impose a functional hierarchy over more anterior ones, a molecular mechanism consistent with 'posterior prevalence'. The posttranscriptional downregulation of more 3' and anteriorly expressed Hox genes by miR-196 constitutes an evolutionarily recent regulatory layer of the highly constrained Hox network, one which recapitulates modes of interactions existing at multiple levels of gene expression.
Noncoding RNAs are encoded by diverse genomes and play many functional roles. MicroRNAs (miRNAs) are endogenous -22-nucleotide noncoding RNAs, derived from larger hairpin precursors, which act by base-pairing to mRNAs to target these transcripts for destruction or translational repression. miRNA genes have been discovered in viral and multicellular genomes. The computational procedure MiRscan was developed to identify miRNA genes conserved in more than one genome and applied to the identification of vertebrate miRNAs. Starting with conserved mouse and human sequences with potential for hairpin formation, and subsequent comparison to fish, 15,000 human genomic loci were identified within aligned regions outside protein coding genes, and ranked according to criteria based on shared features of a training set of the first 50 experimentally verified C. elegans miRNAs. 188 high-scoring candidates, including 74% of human miRNAs known in 2002, were further examined. Fourteen candidate miRNAs were close paralogues of known miRNAs, and 38 candidates were experimentally validated using cDNA libraries of small RNAs made from zebrafish. Of the 38 verified miRNAs, 21 were sequences identified by random cloning and sequencing of cDNA libraries, while 17 were found by applying a directed PCR approach to the same libraries. The miR-196 and miR-10 families are transcribed from genomic loci within clusters of Hox transcription factor genes, and in turn mediate the posttranscriptional repression of neighbouring Hox transcripts, with conserved and extensive targeting of Hox genes located in paralogous groups that are 3' but not 5' of each miRNA locus, relative to the direction of transcription within a given cluster.
by Soraya Yekta.
Ph.D.
Lai, Meng-Jiun. "From sequence to structure and function : an analysis of the A20-like family." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613812.
Full textStaniforth, Gemma Louise. "Characterisation of the function, sequence polymorphism and toxicity of the yeast Rnq1p prion protein." Thesis, University of Kent, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.590014.
Full textSteffen-Munsberg, Fabian [Verfasser]. "Structure– and sequence–function relationships in (S)-amine transaminases and related enzymes / Fabian Steffen-Munsberg." Greifswald : Universitätsbibliothek Greifswald, 2015. http://d-nb.info/1074259610/34.
Full textKoester, Dirk [Verfasser]. "Hierarchy, sequence, function: a contribution to the architecture of the human neurocognitive system / Dirk Koester." Bielefeld : Universitätsbibliothek Bielefeld, 2016. http://d-nb.info/1122285779/34.
Full textHetherington, Simon Lee. "Sequence variation within the platelet adenosine diphosphate receptor genes, platelet function and premature myocardial infarction." Thesis, University of Leicester, 2006. http://hdl.handle.net/2381/27869.
Full textDamry, Adam. "From Protein Sequence to Motion to Function: Towards the Rational Design of Functional Protein Dynamics." Thesis, Université d'Ottawa / University of Ottawa, 2019. http://hdl.handle.net/10393/39211.
Full textChoi, Hyunjin. "An Interdisciplinary Approach: Computational Sequence Motif Search and Prediction of Protein Function with Experimental Validation." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/51762.
Full textPh. D.
Yedida, Venkata Rama Kumar Swamy. "Protein Function Prediction Using Decision Tree Technique." University of Akron / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=akron1216313412.
Full textCrowley, Louis J. "Structure-Function Studies of Conserved Sequence Motifs of Cytochrome b5 Reductase:." Scholar Commons, 2007. https://scholarcommons.usf.edu/etd/682.
Full textGhebru, Alexander. "Metodik för testning av styrsystem." Thesis, KTH, Mikro- och nanosystemteknik, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-109524.
Full textIn this thesis, a general test methodology is created for the testing of control system for pumps. The test methodology is described with models that in a structured way help planning the testing preparation and execution. The methodology is then applied through a stepwise template for how to do the testing in practice. A method for optimizing the numbers of tests is presented. This method will reduce the number of test using the signals with the highest priority that are sent between the controller and pump. A calculation scheme in Excel has been created that brings out the prioritized test procedure for the tester to follow.
Alderson, Rosanna Grace. "Tracking the evolution of function in diverse enzyme superfamilies." Thesis, University of St Andrews, 2016. http://hdl.handle.net/10023/10496.
Full textChan, Yvonne H. "The Complex Role of Sequence and Structure in the Stability and Function of the TIM Barrel Proteins." eScholarship@UMMS, 2011. http://escholarship.umassmed.edu/gsbs_diss/934.
Full textChan, Yvonne H. "The Complex Role of Sequence and Structure in the Stability and Function of the TIM Barrel Proteins." eScholarship@UMMS, 2017. https://escholarship.umassmed.edu/gsbs_diss/934.
Full textLiu, Kwong Ip. "Digital net experimental designs, function interpolations using low discrepancy sequence and goodness of fit tests by discrepancy." HKBU Institutional Repository, 2007. http://repository.hkbu.edu.hk/etd_ra/807.
Full textChia, Nicholas Lee-Ping. "Sequence alignment." Columbus, Ohio : Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1154616122.
Full textCanty, James Ignatius. "Investigating the properties of brown dwarfs using intermediate-resolution spectroscopy." Thesis, University of Hertfordshire, 2015. http://hdl.handle.net/2299/15194.
Full textVogel, Constantin [Verfasser], and Jürgen [Akademischer Betreuer] Pleiss. "Systematic analysis of the sequence-structure-function relationships of thiamine diphosphate-dependent enzymes / Constantin Vogel. Betreuer: Jürgen Pleiss." Stuttgart : Universitätsbibliothek der Universität Stuttgart, 2015. http://d-nb.info/1069815462/34.
Full textBlasingame, Tiffany. "Characterization of a novel aggregate-gland-derived spider silk protein in Latrodectus Hesperus : from sequence to propsoed function." Scholarly Commons, 2009. https://scholarlycommons.pacific.edu/uop_etds/741.
Full textZhang, Zhiling. "The structure and function of troponin T upon metal ion binding and the detection of nucleic acid sequence variations." Thesis, University of North Texas, 2005. https://digital.library.unt.edu/ark:/67531/metadc5565/.
Full textJung, Guhung. "Structure-function analysis of PRD1 DNA polymerase; nucleotide sequence, overexpression and in vitro mutagenesis of the PRD1 DNA polymerase gene." Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184654.
Full textHsieh, Jui-Cheng. "Structure-function analysis of the bacteriophage PRD1 DNA terminal protein: Nucleotide sequence, overexpression, and site-directed mutagenesis of the terminal protein gene." Diss., The University of Arizona, 1990. http://hdl.handle.net/10150/184974.
Full textBuchholz, Patrick Christopher Frank [Verfasser], and Jürgen [Akademischer Betreuer] Pleiss. "Data integration and data mining for the exploration of enzymatic sequence-structure-function relationships / Patrick Christopher Frank Buchholz ; Betreuer: Jürgen Pleiss." Stuttgart : Universitätsbibliothek der Universität Stuttgart, 2018. http://d-nb.info/1173163808/34.
Full textBuchholz, Patrick C. F. [Verfasser], and Jürgen [Akademischer Betreuer] Pleiss. "Data integration and data mining for the exploration of enzymatic sequence-structure-function relationships / Patrick Christopher Frank Buchholz ; Betreuer: Jürgen Pleiss." Stuttgart : Universitätsbibliothek der Universität Stuttgart, 2018. http://d-nb.info/1173163808/34.
Full textLangan, Paul. "X-ray and neutron diffraction studies on the variation of the B conformation of deoxyribonucleic acid as a function of base sequence." Thesis, Keele University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304459.
Full textMalomo, Sunday. "Structure-function properties of hemp seed proteins and protein-derived acetylcholinesterase-inhibitory peptides." Elsevier, 2014. http://hdl.handle.net/1993/30736.
Full textOctober 2015
Roma, Glenn W. "Systematic Analysis of Structure-Function Relationships of Conserved Sequence Motifs in the NADH-Binding Lobe of Cytochrome b5 Reductase." [Tampa, Fla] : University of South Florida, 2008. http://purl.fcla.edu/usf/dc/et/SFE0002558.
Full textSouza, Antonio Marcos de. "A sequencia Fedathi para uma aprendizagem significativa da funÃÃo afim: uma proposta didÃtica com o uso do software geogebra." Universidade Federal do CearÃ, 2015. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14602.
Full textIn the scenario of the Brazilian Public School, every generation of education workers that join in the teaching of Mathematics becomes even more challenging. Consequently, large-scale assessment indicators unveil undesired results relating to learning processes. Considering that, the present work shows a proposal of teaching Affine Function, structured from the assumption of Meaningful Learning Theory and from the methodological proposal of teaching Fedathi Sequence, using as an auxiliary technological resource the software Geogebra. The research was directed to students of the first grade of high school of a State Public School in CearÃ, Brazil. The goal was offer conditions for students, beginning with pre-arranged activities, to build the concept of Affine Function, starting from a problem and, after that, have a clue of this concept by doing simulations in the Geogebra environment, taking the first role in this process. As a methodological procedure of investigation, didactic sessions were achieved, which are classes structured from an environmental and theoretical analysis, following the step of Fedathi Sequence (positioning, maturation, solution and test) that also were considered the programmatic principles of the content (Progressive Differentiation, Integrative Reconciliation, Organizational Sequence and Consolidation) and the Conceptual Maps. During the process of construction and differentiation of concepts by students, the researcher teacher took the so called âhands-in-the-pocket attitudeâ, avoiding give prepared answers to studentsâ questions. The results of qualitative analyses of the didactic sessions and quantitative of pre-test, after-test and questionnaires reveal that: students experienced the process of construction of the concept, taking independent attitude in relation to his/her learning process.; The learned content was represented in a substantive way; The software Geogebra and the conceptual maps facilitated the Progressive Differentiation of the concept of Affine Function, as well as the Integrative Reconciliation of specifications of this concept. Students did not know the software Geogebra, and do not even know another software to study functions before this proposal; the majority of the students successfully learned the exploited concepts. Concluding, the new challenges of learning moves the 21st century teachers to a continuous enrichment of the pedagogic practices from the theories, methodologies and the pedagogic usage of technological resources integrates to the scholar curriculum.
MendonÃa, Adriana Ferreira. "SequÃncia Fedathi na formaÃÃo docente: o conceito de funÃÃo." Universidade Federal do CearÃ, 2017. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=19279.
Full textO objetivo deste trabalho foi verificar se a proposta SequÃncia Fedathi contribui para o ensino do conceito matemÃtico de funÃÃo. Os participantes desta pesquisa foram professores de uma escola da rede pÃblica que fizeram uma formaÃÃo para apreenderem os fundamentos da proposta. Especificamente identificou-se as concepÃÃes docentes acerca deste conceito, contribuindo com sua (re)construÃÃo, discutiu-se elementos histÃricos e epistemolÃgicos e, ainda, analisou-se as mudanÃas na prÃtica docente apÃs a formaÃÃo continuada. A escolha da SequÃncia Fedathi foi associada à sua finalidade de orientar as aÃÃes docentes no processo de elaboraÃÃo e planejamento de aulas e, ainda, porque tem uma conexÃo muito forte com o ensino de conceitos ao valorizar a resoluÃÃo de problemas, a investigaÃÃo, generalizaÃÃo e formalizaÃÃo. As produÃÃes Andrade (2011), Fontenele (2013), Sousa (2015), Pinheiro (2016), Borges Neto (2013, 2017) fazem parte de nosso referencial acerca da proposta Fedathi, enquanto Descartes (1989), Kleiner (1989), Davis e Hersh (1986), Fischbein (2002) Ãvila (2010) compÃem a referencial sobre a intuiÃÃo e a formalizaÃÃo de conceitos. Metodologicamente, esta pesquisa empregou os princÃpios da etnometodologia com suporte na pesquisa participante que permitiu a interaÃÃo do pesquisador com os participantes da pesquisa, flexibilidade para mudanÃas de percurso devido Ãs visÃes dos sujeitos e Ãnfase no processo. Os resultados apontam para mudanÃas significativas nas atitudes dos professores que consideraram que a proposta Fedathi contribui no ensino do conceito de funÃÃo e que pode ser utilizada em contextos diversos propiciando uma aula mais dinÃmica, com a participaÃÃo ativa dos alunos.
Prokop, Jeremy W. "The SRY Gene and Reductionism in Molecular Biology: How to Move from the Benchtop to a Systems Approach." University of Akron / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=akron1373365929.
Full textVan, Niekerk Christopher. "Investigating self-fabrication in the context of artificial chemistries." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/96051.
Full textENGLISH ABSTRACT: This thesis gives a broad overview of what artificial chemistries (ACs) are, a brief review of several ACs and their applications, and an in depth analysis of one speci c AC: the four-bit binary string system. The model designed by Banzhaf [1] for in silico examination was recreated using the Python programming language. The initial motivation was to identify an existing AC that could be used to elucidate the sequence-function relationship, which led to the simultaneous investigation of self-organization in AC systems [7]. The interest in sequence-function relationships stems from their importance for self-production of objects [35]. For self-replication to be possible in larger organizations, the components of the organization must be able to continuously produce themselves [3, 7]. We chose the four-bit binary string system for investigation because of its simple design and implementation, its ability to yield complex results from interactions between a small population of objects, and its analogy to the DNA{RNA{protein organisation. When a population of objects are allowed to continuously interact, self-production and self-organization occur, even in simple arti cial systems [7, 8]. The stability of the emergent organizations depends on the interactions of its components, which must be capable of self-production if they are to maintain the organization [27]. Self-production of objects depends on their sequence-function relationship, which determines their rate of replication when interacting with other objects.
AFRIKAANSE OPSOMMING: Hierdie tesis verskaf `n bree oorsig van die algemene aard van artifisiele chemies (ACs), `n kort opsomming van `n paar ACs en hul toepassings, en `n diepgaande analise van een spesifieke AC: die 4-bis binere stringstelsel. Die model wat Banzhaf [1] ontwerp het vir in silico eksperimentering is hier herskep in die Python programmeringstaal. Die aanvanklike motivering was om `n bestaande AC te identifiseer wat gebruik kon word om die sekwens-funksie verwantskap te ontrafel, en dit het gelei tot die gelyktydige ondersoek van self-organisasie in AC stelsels [7]. Ons belangstelling in sekwens-funksie verwantskappe spruit uit hul belang vir die selfproduksie van objekte [35]. Om selfreplisering in meer omvangryke organisasies moontlik te maak moet die komponente in staat wees om hulself eenstryk te produseer [3, 7]. Ons het `n 4-bis stelsel vir hierdie studie gekies omdat die ontwerp en implementering eenvoudig is, omdat interaksies binne `n klein populasie van objekte komplekse resultate gee, en omdat die stelsel se organisasie analoog aan die DNA-RNA-proteien organisasie is. Wanneer `n populasie van objekte toegelaat word om eenstryk op mekaar te reageer vind self-produksie en self-organisasie vanself plaas, selfs in eenvoudige artifsiele stelsels [7, 8]. Die stabiliteit van die emergente organisasies hang af van die interaksies tussen die komponente, wat self die vermoe tot selfproduksie moet he indien hulle die organisasie in stand wil hou [27]. Selfproduksie van objekte hang af van hul sekwens-funsieverwantskap, wat op hul beurt bepaal hoe vinnig hulle repliseer wanneer in interaksie met ander objekte.
Sangar, Vineet Lesk Arthur M. "Protein function inferences from sequence and structure /." 2008. http://etda.libraries.psu.edu/theses/approved/WorldWideIndex/ETD-2453/index.html.
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