Academic literature on the topic 'Séquençage en cellule unique'
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Journal articles on the topic "Séquençage en cellule unique"
Quesada, Stanislas, and Philippe Jay. "De nouveaux types cellulaires identifiés par séquençage haut débit sur cellule unique." médecine/sciences 32, no. 5 (May 2016): 447–49. http://dx.doi.org/10.1051/medsci/20163205007.
Full textMathieu, Maxime, Amandine Girousse, and Coralie Sengenès. "Et si l’origine des progéniteurs fibro-adipeux contribuait à leur hétérogénéité dans le muscle ?" médecine/sciences 39 (November 2023): 15–21. http://dx.doi.org/10.1051/medsci/2023129.
Full textDariane, Charles, Manon Baures, Julien Anract, Nicolas Barry Delongchamps, Jacques-Emmanuel Guidotti, and Vincent Goffin. "Progéniteurs luminaux prostatiques." médecine/sciences 39, no. 5 (May 2023): 429–36. http://dx.doi.org/10.1051/medsci/2023058.
Full textGrandjean-Closson, Eva, Camille Heckmann, Corentin Le Coz, Isaline Louvet, Matthieu Neri, and Corine Bertolotto. "L’analyse des mélanomes uvéaux primaires à l’aide de la technique de séquençage d’ARN de cellules uniques." médecine/sciences 38, no. 8-9 (August 2022): 737–39. http://dx.doi.org/10.1051/medsci/2022113.
Full textGranier, Sébastien. "Compter les protéines d’une cellule unique." médecine/sciences 23, no. 5 (May 2007): 478–79. http://dx.doi.org/10.1051/medsci/2007235478.
Full textBajaj, Anubha. "The Flowing Cellule-Medullary Thyroid Carcinoma." Journal of Clinical and Biomedical Investigation 1, no. 1 (February 22, 2021): 1–4. http://dx.doi.org/10.52916/jcbi21404.
Full textDelahaye-Duriez, Andrée, Brigitte Benzacken, Michael Johnson, and Enrico Petretto. "Intégration des données de RNAseq sur cellule unique du cerveau." Morphologie 101, no. 335 (December 2017): 240–41. http://dx.doi.org/10.1016/j.morpho.2017.07.006.
Full textRonzitti, Emiliano, Dimitrii Tanese, Alexis Picot, Benoît C. Forget, Valentina Emiliani, and Eirini Papagiakoumou. "Holographie numérique pour la photostimulation de circuits neuronaux." Photoniques, no. 92 (July 2018): 34–37. http://dx.doi.org/10.1051/photon/20189234.
Full textMelzi, Silvia, Guillaume Marcy, Cyril Degletagne, and Christelle Peyron. "Analyses transcriptomiques à cellule unique et à noyau unique de l’hypothalamus dans un état neuro-inflammatoire induit par le LPS." Médecine du Sommeil 20, no. 1 (March 2023): 5–6. http://dx.doi.org/10.1016/j.msom.2023.01.155.
Full textAnderson, Maureen, Ashok Chhetri, Edith Halyk, Amanda Lang, Ryan McDonald, Julie Kryzanowski, Jessica Minion, and Molly Trecker. "Une éclosion de COVID-19 associée à un centre d’entraînement physique en Saskatchewan : leçons pour la prévention." Relevé des maladies transmissibles au Canada 47, no. 11 (November 10, 2021): 538–44. http://dx.doi.org/10.14745/ccdr.v47i11a08f.
Full textDissertations / Theses on the topic "Séquençage en cellule unique"
Foulon, Sophie. "Développement du séquençage ARN ciblé sur cellules uniques en microfluidique de gouttes et applications." Thesis, Paris Sciences et Lettres (ComUE), 2019. http://www.theses.fr/2019PSLET037.
Full textSingle cells technologies were introduced a few years ago and have been dramatically evolving ever since. These technologies have revolutionized biology, making it possible to better understand how heterogeneous cell systems works. For example, they permit to discover and follow cell subtypes, with applications in oncology or neurobiology. We have developed a technology to study the expression profile of genes of interest at the level of a single cell, using droplet-based microfluidics. By limiting the number of genes studied compared to commercial whole-transcriptome technologies, the targeted approach has several potential benefits: gaining deeper sequencing, increasing the number of cells studied, optimizing detection for low levels of expression, while reducing the complexity of data and costs. Targeting is sometimes essential, especially when the RNAs do not carry a generic primer sequence, as in the case of viral RNAs. Two applications are presented: the analysis of inflammation of the immune cells of the brain in the early stages of development, as well as the study of genetic recombination in the virus
Deprez, Marie. "Étude de l’hétérogénéité cellulaire et des dynamiques de régénération de l’épithélium respiratoire sain par analyses des signatures transcriptionnelles sur cellules uniques." Electronic Thesis or Diss., Université Côte d'Azur (ComUE), 2019. http://www.theses.fr/2019AZUR6022.
Full textImprovements made in nucleic acid sequencing and cell handling technologies now offer the opportunity to analyze simultaneously the content of numerous single cells (RNA, DNA, ...) by global and unbiased approaches. This single-cell ‘omics’ revolution provides a new framework to revisit the “Cell Theory”, elaborated over several centuries, and essentially based on morphological and functional features. The many cell modalities now accessible at single- cell level, such as their transcriptome, spatial localization, developmental trajectories, enrich considerably this definition, and set a renewed context to precisely reassess the definition of ‘cell types’, ‘cell states’ as well as their different interactions and fates.My thesis work initially set up ad hoc approaches and statistical framework to analyze appropriately these single-cell data, which deeply differ from standard bulk RNA-seq. High variance, presence of a huge percentage of null values, large volume of data are among the specific characteristics of these datasets. My work was centered on the main experimental model of my host laboratory, e.g. the human airway epithelium. Human airways are lined by a pseudostratified epithelium mainly composed of basal, secretory, goblet and multiciliated cells. Airways also constitute a true cellular ecosystem, in which the epithelial layer interacts closely with immune and mesenchymal cells. This coordination between cells ensures proper defense of the respiratory system and its correct regeneration in case of external aggression and injuries. A better understanding of the operating sequences in normal and physiopathological situations is relevant in pathologies such as chronic obstructive pulmonary disease, asthma or cystic fibrosis.First, I characterized at a single cell level the precise and cell-specific sequence of events leading to functional regeneration of the epithelium, using a 3D model of human cells. I then built a single-cell atlas of the different cell types that are lining healthy human airways from the nose to the 12th generation of bronchi.By applying computational and statistical approaches, I have identified cell lineage hierarchies and was able to reconstruct a comprehensive cell trajectory roadmap in human airways. I not only confirmed previously described cell lineages, but I have also discovered a novel trajectory that links goblet cells to multiciliated cells, identifying novel cell populations and molecular interactors involved in the process of healthy human airway epithelium regeneration. The profiling of 12 healthy volunteers then generated a dataset of 77,969 cells, derived from 35 distinct locations. The resulting atlas is composed of more than 26 epithelial, immune and stromal cell types demonstrating the cellular heterogeneity present in the airways. Its analysis has revealed a strong proximo-distal gradient of expression in suprabasal, secretory, or multiciliated cells between the nose and lung airways. My work has also improved the characterization of rare cells, including “hillock” cells that have been previously described in mice.In conclusion, this work probably represents one of the first single-cell investigations in human airways. It brings original contributions to our understanding of differentiation’s dynamics and cellular heterogeneity in healthy human airways. The resulting resource will be extremely useful for any future single-cell investigators and also for establishing a very useful joint between clinical and biological works. As such, it will constitute a reference in any future project aiming to precisely analyze specific disease conditions
Saviano, Antonio. "Physiopathologie du foie à l'échelle de la cellule unique : caractérisation de l'hétérogénéité cellulaire et identification de nouvelles cibles thérapeutiques dans les maladies hépatiques chroniques et le cancer hépatocellulaire." Thesis, Strasbourg, 2019. http://www.theses.fr/2019STRAJ093.
Full textHepatocellular carcinoma (HCC) is a leading cause of death worldwide and the current treatments are unsatisfactory. One reason is the limited knowledge on the complexity and microenvironment of healthy and diseased liver. To address this gap, we have developed a single cell RNA sequencing (scRNA-seq) pipeline for primary human liver tissues. We have assembled an atlas of human liver cells and compared the scRNA-seq profile of normal liver and HCC. The atlas revealed an unknown heterogeneity within the main populations of liver cells, the transcriptomic zonation of endothelial cells and the existence of an epithelial progenitor in the adult liver capable of differentiating into both cholangiocytes and hepatocytes. ScRNA-seq analysis uncovered the marked cell heterogeneity of HCC, its microenvironment changes at single-cell level and the interactions between tumor cells and hepatitis B virus discovering previously unknown pathways and drivers of hepatocarcinogenesis
Ozier-Lafontaine, Anthony. "Kernel-based testing and their application to single-cell data." Electronic Thesis or Diss., Ecole centrale de Nantes, 2023. http://www.theses.fr/2023ECDN0025.
Full textSingle-cell technologies generate data at the single-cell level. They are coumposed of hundreds to thousands of observations (i.e. cells) and tens of thousands of variables (i.e. genes). New methodological challenges arose to fully exploit the potentialities of these complex data. A major statistical challenge is to distinguish biological informationfrom technical noise in order to compare conditions or tissues. This thesis explores the application of kernel testing on single-cell datasets in order to detect and describe the potential differences between compared conditions.To overcome the limitations of existing kernel two-sample tests, we propose a kernel test inspired from the Hotelling-Lawley test that can apply to any experimental design. We implemented these tests in a R and Python package called ktest that is their first useroriented implementation. We demonstrate the performances of kernel testing on simulateddatasets and on various experimental singlecell datasets. The geometrical interpretations of these methods allows to identify the observations leading a detected difference. Finally, we propose a Nyström-based efficient implementationof these kernel tests as well as a range of diagnostic and interpretation tools
Marcy, Guillaume. "Etude des spécificités transcriptionnelles et de la compétence des progéniteurs neuraux postnataux du cerveau antérieur chez la souris." Thesis, Paris Sciences et Lettres (ComUE), 2018. http://www.theses.fr/2018PSLEP070/document.
Full textDuring development, a remarkable coordination of molecular and cellular events leads to the generation of the cortex, which orchestrates most sensorimotor and cognitive functions. Cortex development occurs in a stepwise manner: radial glia cells (RGs) - the neural stem cells (NSCs) of the developing brain - and progenitor cells from the ventricular zone (VZ) and the subventricular zone (SVZ) sequentially give rise to distinct waves of nascent neurons that form cortical layers in an inside-out manner. Around birth, RGs switch fate to produce glial cells. A fraction of neurogenic RGs that lose their radial morphology however persists throughout postnatal life in the subventricular zone that lines the lateral ventricles. These NSCs give rise to different subtypes of olfactory bulb interneurons and glial cells, according to their spatial origin and location within the postnatal SVZ. These observations raise important unresolved questions on 1) the transcriptional coding of postnatal SVZ regionalization, 2) the potential of postnatal NSCs for cellular regeneration and forebrain repair, and 3) the lineage relationship and transcriptional specificities of postnatal NSCs and of their progenies. My PhD work built upon a previously published comparative transcriptional study of defined microdomains of the postnatal SVZ. This study highlighted a high degree of transcriptional heterogeneity within NSCs and progenitors and revealed transcriptional regulators as major hallmarks sustaining postnatal SVZ regionalization. I developed bioinformatics approaches to explore these datasets further and relate expression of defined transcription factors (TFs) to the regional generation of distinct neural lineages. I then developed a model of targeted ablation that can be used to investigate the regenerative potential of postnatal progenitors in various contexts. Finally, I participated to the development of a pipeline for exploring and comparing select populations of pre- and postnatal progenitors at the single cell level. Objective 1: Transcriptomic as well as fate mapping were used to investigate the relationship between regional expression of TFs by NSCs and their acquisition of distinct neural lineage fates. Our results supported an early priming of NSCs to produce defined cell types depending of their spatial location in the SVZ and identified HOPX as a marker of a subpopulation biased to generate astrocytes. Objective 2: I established a cortical lesion model, which allowed the targeted ablation of neurons of defined cortical layers to investigate the regenerative capacity and appropriate specification of postnatal cortical progenitors. Quantitative assessment of surrounding brain regions, including the dorsal SVZ, revealed a transient response of defined progenitor populations. Objective 3: We developed a transgenic mouse line, i.e. Neurog2CreERT2Ai14, which allowed the conditional labeling of birth-dated cohorts of glutamatergic progenitors and their progeny. We used fate-mapping approaches to show that a large fraction of Glu progenitors persist in the postnatal forebrain after closure of the cortical neurogenesis period. Postnatal Glu progenitors do not accumulate during embryonal development but are produced by embryonal RGs that persist after birth in the dorsal SVZ and continue to give rise to cortical neurons, although with low efficiency. Single-cell RNA sequencing revealed a dysregulation of transcriptional programs, which correlates with the gradual decline in cortical neurogenesis observed in vivo. Altogether, these data highlight the potential of transcriptomic studies to unravel but also to approach fundamental questions such as transcriptional changes occurring in a population of progenitors over time and participating to changes in their fate potential. This knowledge will be key in developing innovative approaches to recruit and promote the generation of selected cell types, including neuronal subtypes in pathologies
Benavente, Diaz Maria. "Investigation of the molecular diversity defining muscle stem cell heterogeneity." Electronic Thesis or Diss., Sorbonne université, 2020. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2020SORUS072.pdf.
Full textAdult skeletal muscle has a remarkable regenerative capacity, being able to recover after repeated trauma. This property depends on the presence of muscle stem cells (MuSCs), which are mostly quiescent in homeostatic conditions, re-enter the cell cycle after injury and proliferate to give rise to committed myoblasts that will eventually fuse to restore the damaged fibres. Numerous studies have investigated the cell state transitions that MuSCs undergo from cell cycle entry to differentiation. Although several genetically modified reporter mice have been generated to study these events, detailed studies on the initiation of differentiation, which is generally defined by expression of the myogenic regulatory factor Myogenin, have been hampered by the lack of a reliable reporter mouse. Therefore, we developed a fluorescent reporter line where differentiating myogenic cells expressing Myogenin are marked by the expression of a tdTomato fluorescent protein. This novel knock-in mouse line allowed us to monitor the kinetics of Myogenin expression during cell differentiation in vitro, and perform preliminary experiments on the behaviour of myogenic cells in vivo by intravital imaging. Although all mouse MuSCs are characterised by the expression of the transcription factor Pax7 and they share several properties, some studies have reported differences in proliferation, engraftment ability, and sensitivity to disease of MuSCs from cranial and limb muscles. To investigate the gene regulatory networks that govern this functional heterogeneity, we have integrated single-cell transcriptomic analyses with cell biology approaches using mouse reporter lines to identify key regulators that confer distinct properties to high performing (extraocular muscles) and lower performing (limb, Tibialis anterior muscle) MuSCs in quiescence and activated states. We identified a delayed lineage progression of extraocular MuSCs in culture that was accompanied with the expression of distinct extracellular matrix remodelling factors and membrane receptors, and we validated the expression of some of these candidates at the protein level. Advanced computational analyses highlighted the dynamics underlying the maintenance of a stem-like progenitor population in extraocular MuSCs, controlled by a singular network of transcription factors acting as a co-regulating module. Taken together, these studies provide novel insights into the mechanisms underlying the differential properties of muscle stem cells in distinct anatomical locations
Cussat-Blanc, Sylvain. "Créatures Artificielles : Développement d'Organismes à partir d'une Cellule Unique." Phd thesis, Université des Sciences Sociales - Toulouse I, 2009. http://tel.archives-ouvertes.fr/tel-00449673.
Full textCaccianini, Laura. "Imagerie de l'architecture dynamique de la chromatine dans la cellule unique." Thesis, Paris Sciences et Lettres (ComUE), 2019. https://tel.archives-ouvertes.fr/tel-02896692.
Full textChromatin structure and cellular function are tightly linked in the nucleus of mammalian cells. Disruption of chromatin spatial organisation dramatically affects the life of a cell and eventually leads to severe pathologies in entire organisms. Two nuclear factors, CTCF and Cohesin, have been found to play a crucial role in the regulation and maintenance of DNA architecture. Huge advancements have been made in the understanding of the mechanisms behind chromatin arrangement but the field is still lacking a dynamic picture at the single cell and single molecule level. This study provide this study provides insight into the dynamics of CTCF and Cohesin through single particle tracking of CTCF and Cohesin dynamics achieved with single molecule tracking in living mouse embryonic stem cells. The interplay between these two factors was studied by looking at Cohesin’s behaviour in the absence of CTCF and in the context of other biological alterations
Bontoux, Nathalie. "Analyse du transcriptome d'une cellule unique à l'aide d'une puce microfluidique." Paris 6, 2006. http://www.theses.fr/2006PA066600.
Full textFarina, Francesca. "Transport de l'ADN dans le cytoplasme d'une cellule eucaryote." Paris 6, 2011. http://www.theses.fr/2011PA066283.
Full textBook chapters on the topic "Séquençage en cellule unique"
Coulon, Antoine, Guillaume Beslon, François Chatelain, Alexandra Fuchs, Olivier Gandrillon, Mathieu Gineste, Jean-Jacques Kupiec, Camila Mejia-Perez, and Andras Páldi. "Chapitre 3. Mécanismes moléculaires et fonction biologique de la variabilité de l’expression génique à l’échelle de la cellule unique : une approche systémique." In Le hasard au cœur de la cellule, 82. Editions Matériologiques, 2011. http://dx.doi.org/10.3917/edmat.kupie.2011.01.0082.
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