Academic literature on the topic 'Septimus'
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Journal articles on the topic "Septimus"
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Egan, Pierce. "The magnificent septimus." Medical Journal of Australia 151, no. 11-12 (December 1989): 715–16. http://dx.doi.org/10.5694/j.1326-5377.1989.tb139657.x.
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Klaus, V. "CONGRESSUS SEPTIMUS INTERNATIONALIS FENNO-UGRISTARUM." Linguistica Uralica 27, no. 2 (1991): 124. http://dx.doi.org/10.3176/lu.1991.2.14.
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Albalawi, Mohammed. "The Manifestations of Woolf’s Life Experiences in Mrs. Dalloway." Advances in Language and Literary Studies 13, no. 1 (February 28, 2022): 16. http://dx.doi.org/10.7575/aiac.alls.v.13n.1.p.16.
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Mrs. Dalloway is one of Virginia Woolf’s greatest achievements. The novel continues to enthuse scholars across the globe, and there are myriad studies through which readers can gain a finer understanding of it. This paper attempts to show how Woolf implants in Mrs. Dalloway a plentiful range of experiences from her life. It argues that in order to have an ample understanding of a character’s state of mind or behavior, emphasis should be placed not only on the text but also on the role of the writer’s personal experiences in its formation. This paper discusses, more specifically, how Woolf’s own experiences are linked to Septimus’s, and showcases that Woolf’s life is a major influence on the story of Septimus.
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Van Wert. "The Early Life of Septimus Smith." Journal of Modern Literature 36, no. 1 (2012): 71. http://dx.doi.org/10.2979/jmodelite.36.1.71.
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Pappas, Nikos, and Michael K. Remson. "Septimus Winner: Two Lives in Music." American Music 22, no. 4 (2004): 589. http://dx.doi.org/10.2307/3592994.
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Poulain, Alexandra. "“A player, a playwright, and the most famous poet in the world”: Highs and Lows in The Player Queen." ABEI Journal 25, no. 2 (December 29, 2023): 33–45. http://dx.doi.org/10.11606/issn.2595-8127.v25i2p33-45.
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Yeats published The Player Queen, a play he had struggled with for more than a decade, in 1922, just a year before he was awarded the Nobel Prize. This article argues despite its appearance of complete wackiness, The Player Queen constitutes a significant landmark in Yeats’s elaboration of his own theatrical aesthetics, as well as a meditation on artistic responsibility—or failure thereof. Why does the poet Septimus fail to communicate his beautiful vision of the Unicorn to anyone, and why does no-one in the play listen to him? On the one hand, the citizens in the play are figures of the incompetent spectators, reminiscent of the audience who rejected Synge at the Abbey. On the other hand, Septimus himself is an incompetent spectator, who is so engrossed in his poetic vision that he fails to pay attention to the momentous change that is really going on before his eyes, although this concerns his own wife Decima, the eponymous Player Queen who comes to replace the real queen. Septimus fails to make himself heard because he is not paying attention to what really matters, he is not fulfilling his duty, as a playwright and a poet, of translating the shapeless chaos of reality into intelligible forms.
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Szabadváry, Tamás. "Septimus Severus „régi-új” medalionja Dunaújvárosból (Intercisa)." Communicationes Archaeologicae Hungariae 2020 (March 3, 2022): 135–44. http://dx.doi.org/10.54640/cah.2020.135.
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A tanulmány célja a Magyar Nemzeti Múzeum által 1907-ben megvásárolt Septimius Severus vörösréz medalion közlése. A Dunaújvárosban (Fejér m., H, korábban: Dunapentele, római kori Intercisa) előkerült ritka érem említés szintjén szerepelt az irodalomban, azonban részletes közlésére eddig nem került sor. A szórvány-lelet pontos lelőhelyének és lelőkörülményeinek azonosítása nehézségbe ütközik, azonban ikonográfiai háttere és kapcsolata a 20. század eleji (illegális) régiségkereskedelemmel a különleges numizmatikai emlékek sorába emeli.
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Soares, Lucas Gabriel. "Como respirar debaixo d’água." Travessias 18, no. 1 (January 30, 2024): e31892. http://dx.doi.org/10.48075/rt.v18i1.31892.
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O presente trabalho pretende analisar possíveis relações biográficas de Virginia Woolf no romance Mrs. Dalloway, de 1925. Na vasta bibliografia literária da autora, apenas em Mrs. Dalloway aparece uma personagem suicida, Septimus Warren-Smith, o que nos leva a refletir sobre as escolhas de Woolf na construção da personagem e em sua apresentação na trama. Segundo o teórico literário, Maurice Blanchot, a literatura é estritamente um espaço de morte, portanto, considerando esse aspecto, a personagem Septimus Warren-Smith poderia apresentar indícios de um evento premonitório: o suicídio da autora alguns anos depois. Virginia Woolf demonstra, por meio de alguns registros, a importância da escrita para sua vida ao utilizar do espaço literário como um exercício catártico de sua relação com o mundo. A intenção do artigo consiste em identificar uma possibilidade de sobrevida da autora a partir da morte de Septimus Warrren-Smith no romance. Para isso, recorreu-se ao conceito de ajustamento criativo da Gestalt-Terapia, através dos estudos de Perls (1977); Hefferline (1997); Goodman (1997), com objetivo de alucidar a relação da autora com seu fazer artístico e sua suposta sobrevida, como também, aos ensaios de Marice Blanchot (1987; 2011) acerca da atividade literária e sua correspondência com a morte. Além disso, com a intenção de possibilitar uma visão abrangente dos fatos envolvidos – estéticos e biográficos – foram necessários somar às ferramentas metodológicas da Teoria da Literatura, como James Wood (2012), e dos Estudos Culturais, como Foucault (2001), aspectos da psicanálise asseverados por Freud (2010) e estudos sobre atributos biográficos elaborados por Bourdieu (2006).
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Thomson, Jean. "Virginia Woolf and the Case of Septimus Smith." San Francisco Jung Institute Library Journal 23, no. 3 (August 2004): 55–71. http://dx.doi.org/10.1525/jung.1.2004.23.3.55.
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Khalid Ali, Maysaloon. "Madness vs. Culture in Virginia Woolf’s Mrs. Dalloway." Journal of Education College Wasit University 2, no. 41 (November 6, 2020): 636–50. http://dx.doi.org/10.31185/eduj.vol2.iss41.1847.
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The purpose of this research is to explain the relationship between madness and the culture of societies, where madness is closely related to cultures. Madness is defined as a group of behaviors characterized by abnormal mental or behavioral patterns. Culture has a tremendous influence on the individual values framework of a society as it is a set of traditional beliefs, rituals, customs and values transmitted and shared in a particular society. Anyone who deviates from these rules will be considered insane.
Virginia Woolf’s Mrs. Dalloway is a novel that expresses her perceptions of the idea of madness throughout history; especially since the writer herself was diagnosed as mentally ill. In this novel, the writer chooses the character of Septimus and his relationship with the outside world. Septimus was responsible, obedient, and loved by his employer, yet his inner world was separated from the outside one. It was exacerbated by the fact that he was distanced from the daily habits of the masses and became a stranger and unfit for normal life. Trapped between the past and the present, he failed to leap over a painful memory, and he gradually fell into a state of madness.
Dissertations / Theses on the topic "Septimus"
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Dickerhof-Borello, Elisabeth. "Ein liber septimus für das Corpus iuris canonici : der Versuch einer nachtridentinischen Kompilation /." Köln : Böhlau, 2002. http://catalogue.bnf.fr/ark:/12148/cb400510838.
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Haynes, I. P. "The Romanization of the alae and cohortes of the Roman imperial army from Augustus to Septimus Severus." Thesis, University of Oxford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358502.
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Hjersing, Charlotte. "Representations of Clarissa and Septimus in Virginia Woolf’s Mrs Dalloway : A deconstructive approach combined with aspects of feminist and psychoanalytical criticism." Thesis, Högskolan Dalarna, Engelska, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:du-4172.
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Ben, Aros Mohamed. "Les développements architecturaux à Leptis Magna pendant l'époque sévérienne (193 – 235)." Thesis, Paris 4, 2013. http://www.theses.fr/2013PA040008.
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Leptis Magna a joué un rôle essentiel dans l'histoire de l'Afrique du Nord. Ce rôle se base sur des données économiques de la ville et de la bonne gestion de ses élites, qui sont ouvertes à la politique de romanisation en adoptant les coutumes et les modèles de l'architecture romaine. Parmi les élites les plus célèbres, celle de la famille des Spetimii : Septime Sévère qui accéda au trône en 193 après J-.C, sous son règne Leptis Magna atteint son apogée et devient la Rome Africaine, en y édifiant les bâtiments sévériens qui font l’objet de cette étude. Le choix de ce sujet se justifie essentiellement par l’importance de l'urbanisme lepcitain et ses caractéristiques à l’époque sévérienne. Qui ont généré des travaux foisonnants en plusieurs langues. Un bilan est désormais nécessaire qui aura pour objectif de mettre en évidence l’importance et l’originalité de cette phase sévérienne: aussi bien pour la ville elle-même que pour l’idéologie impériale, qui s’est manifestée ici de manière éclatante. Nous essayerons ici de connaitre pour quelles raisons Septime Sévère accorda toute son attention à construire ces édifices somptueux pendant une courte période. Parce qu'elle était sa ville natale ? Où bien, présentait-elle un bénéfice avantageux pour Rome ? L'intérêt de ces monuments grandioses datés de l’époque romaine nécessite une recherche historico-scientifique au niveau du tissu urbain : connaitre leur fonctionnement et leur rôle dans la société romaine; étudier leurs composants esthétiques en essayant de trouver les points communs entre eux; également mesurer l’amplitude de la production artistique et sa relation avec le développement politique et économique de la villeLeptis Magna has played a vital role in the history of North Africa.This role is based on the economic data of the city and the good management of its elites who are opened the policy of Romanization by adopting the Roman customs and patterns of Roman architecture. Among elites, most famous are those of the family Septimii which allowed his child, Septimius Severus, came to the throne in 193 AD. Under the reign of this emperor, Leptis Magna reached its peak of prosperity and became the Rome of Africa by setting up a massive constructions program: “The Severan Buildings” are the subject of this study. The choice of this subject is essentially justified by the importance of planning lepcitain characteristics at the Severan period, which generated abundant work in multiple languages. Now an assessment is necessary to highlight the importance and originality of this Severan phase: both for the city itself as well as for imperial ideology, which is conveyed brilliantly. We will try here to know why Septimius Severus gave his full attention to build these magnificent buildings in a short period. Perhaps because it was his hometown? Or was the town an advantageous asset for Rome? The beauty of these great monuments dating from the Roman era requires a historical and scientific research in the urban fabric: To know their operation and their role in Roman society; to study their aesthetic components and to find the common points between them, also to measure the amplitude of the artistic production and its relationships with the political and economic development of the city
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Boubakar, Leila. "Rôle des Septines dans la transmission de traits morphologiques au cours de la neurogenèse des ganglions des racines dorsales." Thesis, Lyon, 2016. http://www.theses.fr/2016LYSE1139.
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La formation des neurites constitue une étape cruciale dans le processus de différenciation neuronale. Cependant, les mécanismes qui permettent de déterminer comment et à quelle position les neurites émergent sont toujours largement méconnus. Nous avons postulé qu'une marque moléculaire pouvait préfigurer la différenciation morphologique. Au cours de ma thèse, j'ai cherché à identifier de nouvelles molécules capables de s'accumuler aux sites d'initiation des neurites et d'en contrôler la protrusion. De manière intéressante, chez la levure, la marque moléculaire qui contrôle le site de protrusion du bourgeon a été caractérisée. Parmi les centaines de protéines contrôlant le site d'initiation chez la levure, les Septines constituent une famille de protéines bien conservée chez les vertébrés. Ces GTPases forment des filaments qui agissent comme barrière de diffusion ou « échafaudage » moléculaire. Au cours de ma thèse, je me suis donc intéressée au rôle des Septines lors de l'initiation axonale dans le modèle des neurones sensoriels de DRG chez l'embryon de poulet. Nous avons pu démontrer qu'aux stades précoces de leur développement, ces neurones formaient deux axones, un au pôle ventral et l'autre au pôle dorsal, indiquant que le nombre et la position des sites d'initiation des axones sont bien contrôlés dans ces neurones. Nous avons, ensuite, démontré que les Septines étaient bien exprimées dans les DRG aux stades précoces du développement. Mes analyses en vidéo-microscopie de la localisation de la septine 7 au cours de la différentiation des neurones de DRG montrent que les Septines s'accumulent au site d'émergence de l'axone, juste avant ou lors de sa formation. L'inhibition des Septines induite par une construction dominant-négative (DN) ou par ajout d'un inhibiteur pharmacologique bloque la formation des axones. De plus, cette inhibition entraine une modification précoce de la morphologie, qui se traduit par l'apparition de cellules multipolaires complexes et de cellules rondes sans prolongement suggérant que, conformément à notre hypothèse, les Septines sont impliquées dans l'initiation des neurites. L'ensemble de ces résultats montre que les Septines régulent la différenciation morphologique précoce des neurones sensorielsNeurite formation is a crucial step of neuronal differentiation. However, the mechanisms that determine how and at which position neurites emerge in the soma are still poorly understood. We postulated that a molecular polarity could prefigure the morphological differentiation, with some molecules that could accumulate at the future site of axon initiation. Interestingly, such molecular polarity has been evidenced in the contest of yeast budding, with bud forming at specific position relatively to the previous bud site. Genome-wide screen identified hundreds of proteins that control bud site location. Among the vertebrate molecules homologous to those involved in budding site selection, we selected the Septins as promising candidates. These GTP-ases form filaments that act as diffusion barriers and molecular scaffolds. We investigated the contribution of Septins to axon initiation using the chick dorsal root ganglion (DRG) neurons as a model. Monitoring of cell morphology in nascent ganglia indicates that DRG neurons form a single axon at the ventral pole and a second one at the dorsal pole and that these axons seem to emerge directly after their last division. This suggests that two initiation sites are selected at opposite pole of the soma.We found that Septins homologous with those controlling budding are expressed in the early DRG developmental stages. My analyses by time-lapse video-microscopy showed that Septin7 accumulate at the site of axon emergence, just before or during its formation.We observed that a pharmacological inhibitor and a dominant-negative construct block axon formation both in vitro and in vivo respectively. Furthermore, blocking Septin function leads to the appearance of uncommon round or sea urchin-like neurons. Thus, Septins appear to regulate early step of morphological differentiation of DRG neurons, possibly by controlling axon initiation site selection
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Sales, Elisa Morandé. "Estudos estruturais do processo de agregação entre proteínas amilóides em solução." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/43/43134/tde-06082012-170235/.
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Septinas fazem parte de uma família de proteínas de ligação ao nucleotídeo guanina que atuam no ciclo de divisão celular e também são amplamente encontradas em doenças neurodegenerativas tais como mal de Parkinson e Alzheimer e em alguns tipos de câncer como leucemia, linfoma e tumores sólidos. Neste trabalho investigamos como a temperatura e a concentração impactam na agregação do domínio GTPase da septina 2 (SEPT2G), podendo levar a formação de bras amilóides, por espalhamento de luz (DLS) e Raios-X a baixos ângulos (SAXS). Resultados de DLS revelaram que a cinética de agregação da proteína é da ordem de segundos para temperaturas maiores que 25ºC. Os dados de SAXS da proteina a 0,5 mg/ml mostraram que a SETP2G é um dímero em solução aquosa a 4ºC e esta conguração se mantém estável por cerca de 1 hora de observação experimental. A 15ºC, os resultados de SAXS revelaram uma coexistência de três populações em solução compostas por 88% de dímeros, 10% de agregados pequenos tipo-cilindros (protobrilas), e 2% de agregados grandes maiores que a resolução da técnica. Após cerca de 30 minutos existe um rearranjo preferencial de dímeros em favor de agregados muito grandes cuja contribuição à curva de espalhamento torna-se 8%. A 25ºC, a porcentagem de dímeros decresce para 70% com uma contribuição de cerca de 30% de agregados grandes já no início das medidas experimentais. Nas temperaturas de 37ºC e 45ºC, dímeros e agregados muito grandes coexistem em solução desde o início das medidas experimentais, cujo equilíbrio se desloca rapidamente tal que após 20 minutos de observação a solução é composta majoritariamente por agregados muito grandes, identicados como estruturas amilóides pela técnica de uorescência da tioavina, que se intercala em estruturas cross-. A 1 mg/mL e temperatura de 4ºC, a proteína permaneceu estável durante cerca de 1 hora de observação sendo que existe um equilíbrio de dímeros (93%) com agregados alongados (contendo cerca de 80 monômeros) em solução. Com o aumento da temperatura para 15ºC, a maioria da população ainda é dimérica. Já a 25ºC, a presença de agregados muito grandes é bem significativa (da ordem de 30% coexistindo com dímeros e oligômeros). A 37ºC e 45ºC existe a formação de grandes agregados similar ao observado para a SEPT2G a 0,5 mg/mL. Em suma, os resultados de SAXS demonstraram que a SEPT2G tem uma cinética muito rápida de agregação a temperatura siológica, acentuada com o aumento de concentração da proteína em solução.Septins are proteins from the GTP-binding family and participate in cell division cycle performing functions such as secretion and cytoskeletal division. They can also be found in neurodegenerative conditions as Alzheimer\'s and Parkinson\'s diseases and some kinds of cancer as leukemia, lymphoma and solid tumors. In this work, we investigated the influence of temperature and concentration on the septin 2 GTPase domain (SEPT2G) aggregation using dynamic light scattering (DLS) and small angle x-ray scattering (SAXS). DLS results revealed the protein aggregation kinetic is around seconds for temperatures above 25ºC. SAXS data of the protein at 0.5 mg/mL showed that SEPT2G is a dimer in aqueous solution at 4_C and this condition is kept stable for approximately one hour of experimental observation. At 15ºC, SAXS results revealed the coexistence of three populations in solution composed by 88% of dimers, 10% of cylinder-like smaller aggregates (protofibrils) and 2% of aggregates bigger than the technique detection. After 30 minutes there is a preferential rearrangement of dimers into very large aggregates which contribution on the scattering curve becomes 8%. At 25ºC, the dimers percentage decreases to 70% with a contribution of circa 30% of bigger aggregates, even at the beginning of data acquisition. At temperatures of 37ºC and 45ºC, dimers and very large aggregates coexist in solution since the beginning of data acquisition, which equilibrium quickly shifts in such a way that after 20 minutes of observation the solution is mostly composed by very large aggregates, indented as amyloid structures by the thioflavine fluorescence technique, which intercalates in the cross- structures. At 1 mg/mL and 4ºC, the protein was stable over 1 hour of observation where an equilibrium of dimers (93%) and elongated structures (composed by approximately 80 monomers) in solution takes place. Increasing the temperature to 15ºC, most of the protein remains dimeric. On the other hand, at 25ºC the very large aggregates contribution is around 30% coexisting with dimers and oligomers. At 37ºC and 45ºC there is the formation of large aggregates, similar to what was observed at 0.5 mg/mL. In conclusion, our SAXS results indicated that the aggregation process of SEPT2G in solution may follow different pathways depending on concentration and temperature.
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Martins, Carla Silva. "Análise das interfaces de interação septina-septina." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-14092017-144809/.
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Septinas pertencem a uma família de proteínas de ligação a GTP e são encontradas em diversos eucariontes, participando de diferentes processos celulares citoesqueléticos. As septinas apresentam um domínio central de ligação a GTP (domínio G) flanqueado por uma região amino-terminal e outra carboxi-terminal. As septinas se caracterizam por interagirem entre si formando heterocomplexos que se polimerizam, constituindo filamentos. A única estrutura resolvida de um complexo de septinas é de um hexâmero, composto por duas subunidades de três septinas humanas diferentes: SEPT7-SEPT6-SEPT2-SEPT2-SEPT6-SEPT7. Esta estrutura revelou que a formação do filamento envolve interações conservadas entre os domínios G, estando o restante da estrutura desordenado no cristal. Além disso, mostrou que dois tipos de interface se alternam ao longo do filamento, as chamadas interfaces G (que incluem a região de ligação do nucleotídeo de duas subunidades) e interfaces NC (que incluem as regiões N e C-terminais do domínio G). Várias evidências sugerem que as regiões C-terminais da proteína sejam as principais responsáveis pela seleção do parceiro correto de interação para montagem dos heterocomplexos. Nesse contexto, buscou-se avaliar a importância das regiões C-terminais na seleção das parceiras SEPT6 e SEPT7 para formar a interface NC, frente ao domínio G. Inicialmente, uma septina quimérica foi produzida de forma a conter o domínio G de SEPT2 e o C-terminal de SEPT6, gerando SEPT2G6C. As proteínas SEPT7GC, SEPT6GC, SEPT2GC e SEPT2G6C foram expressas e purificadas separadamente. Análises de estabilidade térmica e de afinidade proteína-proteína dos pares indicou que a quimera foi capaz de interagir com SEPT7GC, gerando o heterodímero SEPT7GC-SEPT2G6C, mas este não se mostrou tão estável quanto o heterodímero fisiológico. Foi também avaliada a importância da ligação do nucleotídeo para formação da interface G e, para isso, foram construídos os mutantes SEPT2GT78M e SEPT2GD185N, cujos resíduos importantes para hidrólise e ligação do nucleotídeo, respectivamente, foram alterados. A análise de oligomerização por cromatografia de exclusão molecular mostrou deslocamento no volume de eluição das proteínas expressas sozinhas e coexpressas com SEPT6G, indicando que a formação do dímero via interface G depende da ligação do nucleotídeo, mas não da sua hidrólise. Finalmente, foi avaliada a estabilidade térmica e estrutural e a propensão à formação de amilóides do heterodímero SEPT6G-SEPT2G, o qual apresentou maior estabilidade estrutural quando comparado ao homodímero de SEPT2G, mas ainda exibiu alteração de sua estrutura para um estado capaz de ligar Thioflavina-T, sugerindo a formação de amilóides. Entretanto, isso foi observado em temperaturas cerca de 30 ºC acima daquela observada para o homodímero, confirmando a maior estabilidade do heterodímero e sugerindo que a formação da interface G com o parceiro correto pode ser um fator importante na prevenção da formação de estruturas amilóides à temperaturas fisiológicas.Septins belong to a family of GTP binding proteins and are found in several eucaryotes, participating in different cytoskeletal cell processes. The septins have a central GTP binding domain (G domain) flanked by an amino-terminal and a carboxy-terminal regions. The septins are characterized by the ability to interact with each other forming heterocomplexes which polymerize themselves, forming filaments. The only solved structure of a septin complex is a hexamer, formed by two subunits of three different human septins: SEPT7-SEPT6-SEPT2- SEPT2-SEPT6-SEPT7. This structure revealed that the filament arrangement involves conserved interaction between G domains, being the remainder of the structure disordered in the crystal. Moreover, two types of interface alternate along the filament were shown, socalled G interfaces (which include the nucleotide binding region of the two subunits) and NC interfaces (which include the N- and C- terminal regions of G domain). Plenty of evidences suggest that C-terminal regions of the protein are the main responsible for the selection of the correct interaction partner to assembly of heterocomplexes. In this context, it was sought to evaluate the importance of the C-terminal regions in the selection of the partnerships SEPT6 and SEPT7 to form the NC interface, against the G domain. For this, a chimerical septin was designed so that contains the G-domain of SEPT2 and the C-terminal of SEPT6, creating SEPT2G6C. The SEPT7GC, SEPT6GC, SEPT2GC and SEPT2G6C proteins were expressed and purified individually. Thermal stability and protein-protein affinity analysis of the pairs indicated that the chimera was able to interact with SEPT7GC, forming the heterodimer SEPT7GC-SEPT2G6C, which, however, did not show as stable as the physiological heterodimer. The importance of nucleotide binding to the interaction through G interface was also evaluated and, for that, SEPT2 mutants on GTP-domain were constructed, SEPT2T78M and SEPT2D185N, whose important residues in the hydrolysis and linking of nucleotide, respectively, were changed. Oligomerization analysis by size exclusion chromatography showed a shift in the elution volume of proteins expressed alone and coexpressed with SEPT6, indicating that the complexation of proteins to form G interface depends on the nucleotide binding, but not on its hydrolysis. Finally, the thermal and structural stability and the propensity to amyloid formation of heterodimer SEPT6G-SEPT2G were evaluated, which showed greater structural stability when compared to SEPT2 homodimers, but still exhibited alteration of its structure to a state that was able to bind Thioflavin-T, suggesting amyloid formation. However, this was observed at temperatures around 30 ºC above that observed for the homodimer, confirming the greater conformational stability of the heterodimer and suggesting that the formation of G interface with the right partner can be an important factor of the amyloid filament prevention at physiological temperatures.
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Souza, Tatiana de Arruda Campos Brasil de. "Estudos funcionais e estruturais das septinas humanas 6, 8 e 10." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316870.
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Orientador: João Alexandre Ribeiro Gonçalves BarbosaTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Para que a divisão celular ocorra é necessário que uma célula passe por algumas etapas que possibilitem esta divisão. Ao conjunto destes processos denomina-se ciclo celular. A regulação espacial e temporal destes processos é fundamental para a preservação celular e do material genético. Falha neste processo pode levar à morte celular ou a alterações genéticas causando divisão desregulada e crescimento de tumores. Dentre diversas proteínas envolvidas no ciclo celular, encontram-se as septinas. Até o momento, foram encontrados em humanos 14 diferentes genes para septinas. Septinas são proteínas ligadoras de GTP, primeiramente caracterizadas em leveduras, que estão associadas a eventos biológicos importantes em eucariotos. Neste trabalho foram selecionadas três septinas humanas para estudos funcionais e estruturais: septina 6, septina 8 e septina 10. Nossos resultados deram origem a três artigos que descrevem: I) estratégias de clonagem, expressão, purificação e caracterização preliminar da septina humana 8; II) a capacidade das septinas 2, 6, 8 e 11 em ligar e hidrolisar GTP, mas com diferentes níveis de atividade GTPásica, sendo a septina 2 humana capaz de hidrolizar GTP mais rapidamente que as demais septinas e III) a interação entre a septina 10 humana e a proteína Promyelocytic leukemia zinc finger (PLZF), cuja expressão em linhagens celulares hematopoiéticas resultam na supressão do crescimento e interrupção do ciclo celular. A interação da septina 10 com a proteína PLZF foi confirmada in vitro por experimento de pull-down e a localização celular da septina 10 mostra que esta septina é expressa no citoplasma da célula. Além disso, resultados preliminares mostram a relação da ligação de GTP e formação de filamentos pela septina 6 e diversas estratégias para a cristalização das septinas estudadas como: microseeding, streak-seeding, variações de pH, precipitantes e aditivos, metilação de lisinas e screening de tampões, cujos resultados não foram satisfatórios para a resolução de uma estrutura de septina humana
Abstract: A cell passes through some steps to enable for cell division and all these processes are called Cell Cycle. The spatial and temporal regulation of this process is crucial to maintaining cellular and genetic material. Failure in this process can lead to cell death or genetic changes causing division and unregulated growth of tumors. Among several proteins involved in cell cycle, there are the septins. To date, 14 different human genes coding for septins were found. Septins are GTP binding proteins first characterized in yeast, which are associated with important biological events in eukaryotes. In this study we selected three human septins to study functionally and structurally: septin 6, septin 8 and septin 10. Our results led to three articles that describe: i) strategies for cloning, expression, purification and preliminary characterization of human septin 8; ii) the ability of septin 2, 6, 8 and 11 to bind and hydrolyze GTP, but with different levels GTPase activity, human septin 2 is capable of hydrolyzing GTP faster than the other septins and III) the interaction between the human septin 10 and promyelocytic leukemia zinc finger protein (PLZF), whose expression in hematopoietic cell lines results in growth suppression and cell cycle arrest. The interaction between septin 10 and PLZF was confirmed by in vitro pull down assay and the cellular localization of septin 10 shows that this septin is expressed in the cell cytoplasm. Furthermore, our studies preliminary results show the relation of GTP binding and filament formation of the septin 6 and several strategies for the crystallization of septins as micro-seeding, streak-seeding, variations in pH, precipitants and additives, methylation of lysine and screening of buffers, but the results were not satisfactory for the resolution of a human septin structure
Doutorado
Genetica de Microorganismos
Doutor em Genetica e Biologia Molecular
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Morais, Sinara Teixeira do Brasil. "Estudos estruturais da SEPT8 e análise de suas interações com SEPT5 e SEPT7." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-05082014-082458/.
Full textAbstract:
Septinas são proteínas que ligam GTP e interagem entre si formando heterocomplexos, os quais formam filamentos e estruturas de maior nível de organização. Tais filamentos, além de se mostrarem importantes durante a citocinese também podem estar envolvidos em outros processos celulares tais como determinação da polaridade celular e reorganização do citoesqueleto. As septinas, inicialmente descobertas em Saccharomyces cerevisiae, já foram identificadas também em fungos, algas-verdes, mamíferos, porém nunca em plantas. Tipicamente, septinas apresentam três domínios estruturais compostos por um domínio central de ligação ao GTP flanqueado por um N-terminal variável e um C-terminal que pode conter sequências do tipo coiled-coil. Este trabalho teve como objetivo a caracterização do domínio de ligação a GTP da septina 8 humana (SEPT8G) por meio de estudos biofísicos. Nesse sentido, SEPT8G foi eficientemente produzida em E. coli, tendo seu estado dimérico em solução confirmado por cromatografia de exclusão molecular. Diferentemente das outras septinas já reportadas, mesmo dimérica a SEPT8G apresentou-se na forma apo. Ensaios de atividade GTPásica foram realizados, confirmando a incapacidade dessa septina em hidrolisar o GTP. Ainda, análises de estabilidade térmica por Dicroísmo Circular revelaram que a presença do íon magnésio leva à diminuição de sua estabilidade estrutural. Baseando-se em resultados prévios de interação obtidos pela técnica do duplo-híbrido em leveduras, estudos voltados à análise da interação entre as septinas 7 e 8 e, posteriormente, entre as septinas 5, 7 e 8 foram realizados. A co-purificação do complexo formado pelas septinas 7 e 8 mostrou-se dependente da região C-terminal completa de SEPT7 de modo que, quando ausente, a interação mostrou-se expressivamente prejudicada. Já o complexo formado pelas septinas 5/7/8 foi obtido contendo as três proteínas em frações equimolares e solúveis, disponibilizando assim um novo heterocomplexo de septinas para ensaios funcionais e estruturais.Septins are GTP-binding proteins that interact with each other to form heterocomplexes, which form filaments and higher order structures. These filaments are important for cytokinesis and may be involved in other cellular processes such as the determination of cell polarity and cytoskeleton reorganization. The septins, initially discovered in Saccharomyces cerevisiae, have also been identified in fungi, green algae, mammals but not in plants. Typically, septins have three structural domains comprising a central GTP binding domain flanked by a variable N-terminal and a C-terminal which can contain coiled-coil structures. This work sought to characterize the GTP-binding domain of the human septin 8 (SEPT8G) through biophysical studies. Accordingly, SEPT8G was efficiently produced in E. coli, and its dimeric state in solution was confirmed by size exclusion chromatography. Compared to other septins previously reported, the dimeric SEPT8G presented itself as an apo-protein. GTPase activity assays were performed, confirming the inability of this septin to hidrolyse GTP. Additionally, thermal stability analyses by Circular Dichroism showed that the presence of the magnesium ion leads to a decrease of structural stability. Considering previous results of septins interactions from yeast two-hybrid experiments, we have analyzed the interaction between the septins 7, 8 and subsequently among septins 5, 7 and 8. Co-purification of the complex formed by the septins 7 and 8 showed to be dependent on the complete SEPT7 C-terminal region so that, when absent, the interaction was significantly impaired. The obtained complex formed by septins 5/7/8 contained the three proteins in soluble and equimolar fractions, providing a new septin heterocomplex for functional and structural studies.
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Castro, Danielle Karoline Silva do Vale. "Reconhecimento molecular de septinas: estudos da interface entre SEPT7 e SEPT12." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/75/75133/tde-04122018-135816/.
Full textAbstract:
A família das septinas caracteriza-se pela capacidade de ligar nucleotídeos de guanina e de se associarem formando filamentos. Diversas funções biológicas têm sido reportadas para esses filamentos e sua dissociação pode estar relacionada a patologias. A septina 12 humana expressa especificamente em testículos, foi identificada em filamentos que compõem o annulus do espermatozoide, cuja integridade está relacionada com a morfologia deste. Embora muitos estudos tenham sido reportados, vários aspectos das bases moleculares e fisiológicas de sua função e automontagem permanecem desconhecidos. Neste trabalho, procurou-se obter informações estruturais para o domínio de ligação ao nucleotídeo da SEPT12 (SEPT12G), do mutante SEPT12GT89M e do heterodímero SEPT7-SEPT12. A expressão destas proteínas foi realizada em células de E. coli da linhagem Rosetta(DE3) pelos vetores de expressão pET28a(+) e pETDuet-1. As etapas de purificação foram cromatografia de afinidade e exclusão molecular. A proteína SEPT12G foi submetida à avaliação do estado oligomérico, fluorescência intrínseca, ensaios de conteúdo de nucleotídeo, atividade GTPásica e transição térmica. O heterodímero SEPT7-SEPT12 foi submetido à avaliação do estado oligomérico e conteúdo de nucleotídeo. Ensaios de cristalização foram realizados para todas as proteínas. A coleta de dados realizada na linha I24 do Diamond Light Source (Didcot, Inglaterra) resultou em conjuntos de dados de alta resolução para a SET12G, SEPT12GT89M e baixa resolução para a SEPT7NGc. Os estudos biofísicos mostraram que a SEPT12G foi obtida em sua forma nativa ou, seja, capaz de ligar e hidrolisar o nucleotídeo GTP e que o heterodímero obtido apresentou ambas as proteínas. As estruturas cristalográficas foram resolvidas e permitiram realizar observações importantes para o grupo I das septinas humanas (SEPT3, SEPT9 e SEPT12). Para a SEPT12 pôde-se observar como a mudança que ocorre no motivo G4 pode alterar a estabilidade da interface G. No contexto do grupo I esta estrutura permitiu concluir que todas as proteínas deste subgrupo podem formar duas interfaces NC, dos tipos aberta e fechada. Além disso, reforçou a observação da orientação diferencial da hélice α5\', cuja função ainda não está esclarecida, mas sem dúvidas é um diferencial que caracteriza este grupo, possivelmente relacionado com a ancoragem da região polibásica na conformação aberta. A estrutura cristalográfica do mutante SEPT12T89M permitiu observar que a mutação levou a uma alteração na primeira esfera de coordenação do íon Mg2+, mudança que interrompe o mecanismo do switch universal e deixa a proteína catalíticamente inativa. Por fim, o estudo cristalográfica do complexo entre a SEPT12 e SEPT7 não foi possível, uma vez que todas as tentativas resultaram em cristais contendo apenas a SEPT7, o que pode ser consequência da precipitação da SEPT12 ou da condição de cristalização utilizada, que desestabiliza o heterodímero.The septin family of proteins is characterized by their ability to bind guanine nucleotides and associate into filaments. Several biological functions have been reported for these filaments and their dissociation may be related to pathologies. Human septin is 12 specifically expressed in testes and has been identified in filaments that form the sperm annulus, whose integrity is related to its morphology. Although many studies have been reported, the molecular and physiological bases of septin filament function and self-assembly have yet to be completely elucidated. This study aims to obtain structural information for the nucleotide binding domain of SEPT12 (SEPT12G), the SEPT12GT89M mutant and the SEPT7-SEPT12 heterodimer. Expression of these proteins was performed in E. coli Rosetta(DE3) strain using the pET28a (+) and pETDuet-1 expression vectors. Purification was performed by affinity and size exclusion chromatography. The SEPT12G protein was submitted to an evaluation of its oligomeric state, intrinsic fluorescence, nucleotide content, GTPase activity and thermal transition. The oligomeric state and nucleotide content of SEPT7-SEPT12 was also evaluated. Crystallization assays were performed for all proteins. Data collection on line I24 of the Diamond Light Source (Didcot, England) resulted in high-resolution data sets for SET12G and SEPT12GT89M but only low resolution data for the SEPT7NGc. Biophysical studies showed that SEPT12G was obtained in its native form or, in other words, capable of binding and hydrolyzing GTP and that the purified heterodimer presented both proteins. The crystallographic structures were solved by molecular replacement allowing the identification of features characteristics of the group I septins (SEPT3, SEPT9 and SEPT12). The structure also confirmed that all the proteins of this group are able to form two different NC interfaces: open and closed. In addition, it reinforced the observation that the α5\' helix assumes a different orientation, whose function has not yet been clarified, but without doubt is a characteristic of this group which may be related to anchoring the polybasic regions whilst in the open conformation. The SEPT12T89M mutant crystal structure shows that the first shell coordination of the Mg2+ ion is altered, leading to an interruption of the universal switch mechanism and a consequent lack of catalytic activity. Finally, structural studies of the interaction between SEPT12 and SEPT7 were not possible, since all attempts resulted in crystals containing only SEPT7. This may be a consequence of SEPT12 precipitation or the crystallization condition used, destabilizing the heterodimeric interface.
Books on the topic "Septimus"
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Janet, Zoro, and Royal National Life-Boat Institution for the Preservation of Life from Shipwreck (Great Britain), eds. Septimus: Rescues again. Fowey: Carlton House, 2001.
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Digby, Sheila. Septimus comes to town. Fowey: Carlton House, 1998.
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Digby, Sheila. Septimus meets the smugglers. Fowey: Carlton House, 1998.
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Sage, Angie. The magykal papers: Septimus Heap. London: Bloomsbury, 2009.
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Digby, Sheila. Septimus to the rescue: Story. Fowey: Carlton House Publications, 1996.
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Septimus. Barcelona: Debolsillo, 2007.
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Locke, William John. Septimus. Dodo Press, 2008.
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Locke, William John. Septimus. Kessinger Publishing, 2004.
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Locke, William John. Septimus. Classic Books, 2000.
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Septimus. Circulo de Lectores, 2005.
Find full textBook chapters on the topic "Septimus"
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Puu, Tönu. "CAUDEX SEPTIMUS." In Disequilibrium Economics, 271–73. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-74415-5_17.
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Harcourt, G. C. "Keith Septimus Frearson, 18 September 1922–2 February 2000: A Memoir and a Tribute (2000)." In On Skidelsky’s Keynes and Other Essays, 290–93. London: Palgrave Macmillan UK, 2012. http://dx.doi.org/10.1057/9780230348646_17.
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Parker, H. M. D., and B. H. Warmington. "Septimius Severus." In A History of the Roman World from A.D. 138 to 337, 55–79. London: Routledge, 2024. http://dx.doi.org/10.4324/9781003480112-7.
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Daviaud, F., and F. Pène. "Choc septique." In Références en réanimation. Collection de la SRLF, 255–72. Paris: Springer Paris, 2013. http://dx.doi.org/10.1007/978-2-8178-0389-0_16.
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Leclerc, F., A. Botte, M. É. Lampin, R. Cremer, and S. Leteurtre. "Choc septique." In Réanimation pédiatrique, 51–75. Paris: Springer Paris, 2013. http://dx.doi.org/10.1007/978-2-8178-0407-1_3.
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Mathieu, Solène, Nicolas Ducrocq, Antoine Kimmoun, Céline Bridey, and Bruno Lévy. "Choc septique." In Références en réanimation. Collection de la SRLF, 3–7. Paris: Springer Paris, 2014. http://dx.doi.org/10.1007/978-2-8178-0503-0_1.
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Columella], Columella [Lucius Junius Moderatus. "Liber Septimus." In Oxford Classical Texts: L. Iuni Moderati Columellae: Res Rustica; Incerti Auctoris: Liber de Arboribus, edited by R. H. Rodgers, 284. Oxford University Press, 2010. http://dx.doi.org/10.1093/oseo/instance.00148292.
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"LIBER SEPTIMUS." In Die Pseudoklementinen, edited by Bernhard Rehm and Georg Strecker. Berlin, New York: DE GRUYTER, 1994. http://dx.doi.org/10.1515/9783110887945.197.
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"LIBER SEPTIMUS." In Confessionum Libri XIII, 124–52. B. G. Teubner, 1996. http://dx.doi.org/10.1515/9783110948646.124.
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"LIBER SEPTIMUS." In Polistoria de virtutibus et dotibus Romanorum, 188–201. B. G. Teubner, 1995. http://dx.doi.org/10.1515/9783110966268.188.
Full textConference papers on the topic "Septimus"
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Zhang, Hengliang. "The Death of Septimus and Virginia Woolf’s Attitude Towards Rationality." In 2020 International Conference on Language, Communication and Culture Studies (ICLCCS 2020). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/assehr.k.210313.067.
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Hong, Dezhi, Ben Zhang, Qiang Li, Shahriar Nirjon, Robert Dickerson, Guobin Shen, Xiaofan Jiang, and John Stankovic. "SEPTIMU." In the 11th international conference. New York, New York, USA: ACM Press, 2012. http://dx.doi.org/10.1145/2185677.2185727.
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"SePTIS Track Committee." In 2008 IEEE International Conference on Signal Image Technology and Internet Based Systems. IEEE, 2008. http://dx.doi.org/10.1109/sitis.2008.111.
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Hu, Pan, Robert Dickerson, John A. Stankovic, Guobin Shen, Xiaofan Jiang, Shao-fu Shih, Donghuan Lu, et al. "Septimu2- earphones for continuous and non-intrusive physiological and environmental monitoring." In the 10th ACM Conference. New York, New York, USA: ACM Press, 2012. http://dx.doi.org/10.1145/2426656.2426722.
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Vorakunthada, Y., and W. Lilitwat. "Clostridium Septicum Bacteremia Presenting as a Para-Neoplastic Sepsis." In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a6585.
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Hong, Dezhi, Ben Zhang, Qiang Li, Shahriar Nirjon, Robert Dickerson, Guobin Shen, Xiaofan Jiang, and John A. Stankovic. "Demo abstract: SEPTIMU — Continuous in-situ human wellness monitoring and feedback using sensors embedded in earphones." In 2012 ACM/IEEE 11th International Conference on Information Processing in Sensor Networks (IPSN). IEEE, 2012. http://dx.doi.org/10.1109/ipsn.2012.6920929.
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BRARA, AHMED, and NAIMAT OULOUM. "Deterioration patterns of building limestone in the Septimius Severus family Temple in the Romain ruins of Djemila Algeria." In Fourth International Conference on Advances in Civil, Structural and Environmental Engineering - ACSEE 2016. Institute of Research Engineers and Doctors, 2016. http://dx.doi.org/10.15224/978-1-63248-114-6-74.
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Mouraret, A., E. Gerard, J. Le Gall, and R. Curien. "Ostéonécrose du prémaxillaire consécutive à une coagulation intravasculaire disséminée : à propos d’un cas." In 66ème Congrès de la SFCO. Les Ulis, France: EDP Sciences, 2020. http://dx.doi.org/10.1051/sfco/20206603011.
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La coagulation intravasculaire disséminée (CIVD) est une pathologie rare correspondant à l’activation systémique de la cascade de coagulation. Les thrombi fibrineux formés, auxquels s’ajoute un éventuel vasospasme, conduisent à l’ischémie et à la nécrose des tissus. La CIVD est fréquemment associée à un traumatisme, une blessure, une complication obstétrique ou à un choc septique. De nombreux cas d’ostéonécrose dus à une CIVD ont été décrits dans la littérature orthopédique (principalement concernant la tête fémorale), mais très peu de cas sont retrouvés concernant la région maxillo-mandibulaire. Une patiente de 83 ans se présente en consultation pour l’apparition récente de mobilités au niveau du bloc incisivo-canin maxillaire. L’interrogatoire révèle le diagnostic deux mois auparavant d’une leucémie myéloïde aigue, découverte de manière fortuite suite à un choc septique à E. Coli. Les bilans biologiques réalisés au moment du sepsis étaient en faveur d’une coagulation intravasculaire disséminée (CIVD). Quelques jours après, la patiente se plaignait de douleurs et de mobilités anormales localisées sur les dents antérieures maxillaires. Les examens cliniques et radiographiques (orthopantomogramme et cone beam) objectivent une nécrose osseuse du prémaxillaire limitée par les canines, avec un séquestre indépendant du reste du maxillaire. L’étude d’imputabilité conclut à la responsabilité de la CIVD en l’absence d’autres facteurs déclenchants et en raison d’une chronologie concordante. Le diagnostic de nécrose consécutive à une CIVD est alors posé. La prise en charge consiste en une séquestrotomie sous anesthésie générale et fermeture muqueuse. Un suivi régulier est mis en place avec une évolution favorable. Le prémaxillaire possède une vascularisation très riche assurée par les branches terminales de l’artère carotide externe, et par l’artère maxillaire. Les nécroses avasculaires sont par conséquent rares dans cette région. Cette vascularisation abondante explique le peu de cas décrits dans la littérature. On retrouve deux cas de nécrose consécutive à une CIVD au niveau de la mandibule et deux cas pour le prémaxillaire.
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"SePTIS: Workshop on Security and Privacy in Telecommunications and Information System." In Internet-Based Systems (SITIS 2009). IEEE, 2009. http://dx.doi.org/10.1109/sitis.2009.88.
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Amoakoh, Josephine. ""Give Me the Oars": Revisiting Septima Poinsette Clark's English Literacy Pedagogy With Gullah-Speaking Children (1916–1919)." In 2020 AERA Annual Meeting. Washington DC: AERA, 2020. http://dx.doi.org/10.3102/1583611.
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