Journal articles on the topic 'Sentinel prey'

Abstract Diverse and robust predator communities are important for effective prey suppression in natural and managed communities. Ants are ubiquitous components of terrestrial systems but their contributions to natural prey suppression is relatively understudied in temperate regions. Growing evidence suggests that ants can play a significant role in the removal of insect prey within grasslands, but their impact is difficult to separate from that of nonant predators. To test how ants may contribute to prey suppression in grasslands, we used poison baits (with physical exclosures) to selectively reduce the ant population in common garden settings, then tracked ant and nonant ground predator abundance and diversity, and removal of sentinel egg prey for 7 wk. We found that poison baits reduced ant abundance without a significant negative impact on abundance of nonant ground predators, and that a reduction in ant abundance decreased the proportion of sentinel prey eggs removed. Even a modest decrease (~20%) in abundance of several ant species, including the numerically dominant Lasius neoniger Emery (Hymenoptera: Formicidae), significantly reduced sentinel prey removal rates. Our results suggest that ants disproportionately contribute to ground-based predation of arthropod prey in grasslands. Changes in the amount of grasslands on the landscape and its management may have important implications for ant prevalence and natural prey suppression services in agricultural landscapes.

The sentinel behaviour of 38 Arabian babbler adult floaters, who lived alone within a territory belonging to a foreign group, was studied and compared with their own sentinel behaviour in the past, when they were group members. All floaters acted as sentinels and uttered ‘alarm calls’. This suggests that sentinel activity is due at least, in part, to selfish motives. Floaters sentinelled less than they did as group members, with the decrease in sentinel activity sharper for ex-dominants than for ex-subordinates. One possible explanation for these differences is that sentinel activity is aimed not only at detecting predators, but also at detecting foreign conspecifics. Within a group, the latter incentive is stronger for breeding dominants than for subordinates, whereas all floaters alike may be trying to detect the owners of the territory in which they were roaming but also to avoid being detected by them. Other possible explanations are that floaters have less time and energy for sentinel activity because they are weaker or because foraging is more difficult in a foreign territory. This may be especially so for dominants who used to enjoy privileged access to food in their group. No significant difference was found in the rate of sentinels' ‘alarm calls’ between floaters and group members, suggesting that their main purpose is predator–prey communication, of which warning groupmates may be a side benefit.

Complex landscapes including semi-natural habitats are expected to favour natural enemies thereby enhancing natural pest biocontrol in crops. However, when considering a large number of situations, the response of natural biocontrol to landscape properties is globally inconsistent, a possible explanation being that local agricultural practices counteract landscape effects. In this study, along a crossed gradient of pesticide use intensity and landscape simplification, we analysed the interactive effects of landscape characteristics and local pesticide use intensity on natural biocontrol. During 3 years, using a set of sentinel prey (weed seeds, aphids and Lepidoptera eggs), biocontrol was estimated in 80 commercial fields located in four contrasted regions in France. For all types of prey excepted weed seeds, the predation rate was influenced by interactions between landscape characteristics and local pesticide use intensity. Proportion of meadow and length of interface between woods and crops had a positive effect on biocontrol of aphids where local pesticide use intensity was low but had a negative effect elsewhere. Moreover, the landscape proportion of suitable habitats for crop pests decreased the predation of sentinel prey, irrespectively of the local pesticide use intensity for weed seeds, but only in fields with low pesticide use for Lepidoptera eggs. These results show that high local pesticide use can counteract the positive expected effects of semi-natural habitats, but also that the necessary pesticide use reduction should be associated with semi-natural habitat enhancement to guarantee an effective natural biocontrol.

Urbanization is one of the most important global trends which causes habitat reduction and alteration which are, in turn, the main reasons for the well-documented reduction in structural and functional diversity in urbanized environments. In contrast, effects on ecological mechanisms are less known. Predation is one of the most important ecological functions because of its community-structuring effects. We studied six forest habitats along a riverside urbanization gradient in Szeged, a major city in southern Hungary, crossed by the river Tisza, to describe how extreme events (e.g., floods) as primary selective pressure act on adaptation in riparian habitats. We found a generally decreasing predation pressure from rural to urban habitats as predicted by the increasing disturbance hypothesis (higher predator abundances in rural than in urban habitats). The only predators that reacted differently to urbanization were ground active arthropods, where results conformed to the prediction of the intermediate disturbance hypothesis (higher abundance in moderately disturbed suburban habitats). We did not find any evidence that communities exposed to extreme flood events were preadapted to the effects of urbanization. The probable reason is that changes accompanied by urbanization are much faster than natural landscape change, so the communities cannot adapt to them.

Tea (Camellia sinensis) is an important food product with thousands of years of human use. Being a non-washable food, no pesticide residues are allowed, which increases the importance of natural means of plant protection. Predation, a component of natural pest control, is an important contributor to this, but its level and sustainability are not known in most of the areas of tea production. We quantified predation intensity using the artificial sentinel prey method in a tea-growing landscape containing remnants of the original forest vegetation in Fujian Province, China. The most common predators were chewing arthropods (49.8% of predation events) and birds (48.1%). Overall, predation rates at the edges of forest fragments (18.9% d-1) were lower than either in fragment interiors (25.4%d−1) or in the surrounding tea plantations (19.2–24.1%d-1). Arthropod predation was higher inside, and at the edge of, forest fragments than within plantations, and generally decreased with increasing distance from a fragment edge, indicating limited spillover of arthropod predators from the native habitat remnants to the cultivated matrix at the local scale. Bird predation, though, showed a different trend: it was lower on the inside of forest fragments than in the tea planation, and bird attack rates increased at increasing distances (up to 40 m) from the forest fragment edge. We also found a reciprocal relationship between attack rates by birds and arthropods, suggesting intra-guild predation. Measures protecting arthropod natural enemies could increase the combined pest suppression effect, contributing to pesticide-free tea production in China.

There is an urgent need for a better understanding of animal migratory ecology under the influence of climate change. Most current analyses require long-term monitoring of populations on the move, and shorter-term approaches are needed. Here, we analysed the ecological drivers of seabird migration within the framework of the energyscape concept, which we defined as the variations in the energy requirements of an organism across geographical space as a function of environmental conditions. We compared the winter location of seabirds with their modelled energy requirements and prey fields throughout the North Atlantic. Across six winters, we tracked the migration of 94 little auks ( Alle alle ), a key sentinel Arctic species, between their East Greenland breeding site and wintering areas off Newfoundland. Winter energyscapes were modelled with Niche Mapper™, a mechanistic tool which takes into account local climate and bird ecophysiology. Subsequently, we used a resource selection function to explain seabird distributions through modelled energyscapes and winter surface distribution of one of their main prey, Calanus finmarchicus . Finally, future energyscapes were calculated according to IPCC climate change scenarios. We found that little auks targeted areas with high prey densities and moderately elevated energyscapes. Predicted energyscapes for 2050 and 2095 showed a decrease in winter energy requirements under the high emission scenario, which may be beneficial if prey availability is maintained. Overall, our study demonstrates the great potential of the energyscape concept for the study of animal spatial ecology, in particular in the context of global change.

Accumulating scientific evidence has demonstrated widespread shifts in the biological seasons. These shifts may modify seasonal interspecific interactions, with consequent impacts upon reproductive success and survival. However, current understanding of these impacts is based upon a limited number of studies that adopt a simplified ‘bottom-up’ food-chain paradigm, at a local scale. I argue that there is much insight to be gained by widening the scope of phenological studies to incorporate food-web interactions and landscape-scale processes across a diversity of ecosystem types, with the ultimate goal of developing a generic understanding of the systems most vulnerable to synchrony effects in the future. I propose that co-location of predator and prey phenological monitoring at sentinel sites, acting as research platforms for detailed food-web studies, experimentation and match-up with earth observation data, would be an important first step in this endeavour.

AbstractBirds of prey, owls and falcons are widely used as sentinel species in raptor biomonitoring programmes. A major current challenge is to facilitate large-scale biomonitoring by coordinating contaminant monitoring activities and by building capacity across countries. This requires sharing, dissemination and adoption of best practices addressed by the Networking Programme Research and Monitoring for and with Raptors in Europe (EURAPMON) and now being advanced by the ongoing international COST Action European Raptor Biomonitoring Facility. The present perspective introduces a schematic sampling protocol for contaminant monitoring in raptors. We provide guidance on sample collection with a view to increasing sampling capacity across countries, ensuring appropriate quality of samples and facilitating harmonization of procedures to maximize the reliability, comparability and interoperability of data. The here presented protocol can be used by professionals and volunteers as a standard guide to ensure harmonised sampling methods for contaminant monitoring in raptors.

Non-crop vegetation around farmland can be valuable habitats for enhancing ecosystem services but little is known of the importance of field margins in supporting natural enemies of insect pests in tropical agriculture. This study was conducted in smallholder bean fields in three elevation zones to assess the importance of field margin vegetation to natural enemy populations and movement to the bean crop for biological pest control. The pests and natural enemies were assessed using different coloured water pan traps (to ensure the capture of insects with different colour preferences) and the interactions of the two arthropod groups with the margin vegetation and their movement to the bean crop were monitored using fluorescent dye. Sentinel plants were used to assess predation and parasitism levels. A total of 5003 natural enemies were captured, more in the field margin than within the bean field for low and mid elevation zones, while in the high elevation zone, they were more abundant within the bean field. Pests were more abundant in the crop than margins for all the elevation zones. The use of a dye applied to margin vegetation demonstrated that common natural enemy taxa moved to the crop during the days after dye application. The proportion of dye-marked natural enemies (showing their origin to be margin vegetation) sampled from the crop suggest high levels of spatial flux in the arthropod assemblage. Aphid mortality rates (measured by prey removal and parasitism levels on sentinel plants) did not differ between the field edges and field centre in any of the three elevation zones, suggesting that for this pest taxon, the centre of the fields still receive comparable pest control service as in the field edges. This study found that field margins around smallholder bean fields are useful habitats to large numbers of natural enemy taxa that move to adjacent crops providing biological pest control service.

Invertebrates perform many vital functions in agricultural production, but many taxa are in decline, including pest natural enemies. Action is needed to increase their abundance if more sustainable agricultural systems are to be achieved. Conservation biological control (CBC) is a key component of integrated pest management yet has failed to be widely adopted in mainstream agriculture. Approaches to improving conservation biological control have been largely ad hoc. Two approaches are described to improve this process, one based upon pest natural enemy ecology and resource provision while the other focusses on the ecosystem service delivery using the QuESSA (Quantification of Ecological Services for Sustainable Agriculture) project as an example. In this project, a predictive scoring system was developed to show the potential of five seminatural habitat categories to provide biological control, from which predictive maps were generated for Europe. Actual biological control was measured in a series of case studies using sentinel systems (insect or seed prey), trade-offs between ecosystem services were explored, and heatmaps of biological control were generated. The overall conclusion from the QuESSA project was that results were context specific, indicating that more targeted approaches to CBC are needed. This may include designing new habitats or modifying existing habitats to support the types of natural enemies required for specific crops or pests.

Habitat use by sexually dimorphic Bar-tailed Godwits (Limosa lapponica) was examined in an Australian estuary during the midwintering (November–December) and premigratory (March) periods of a nonbreeding season. In an area with two available habitats, Zostera seagrass beds and unvegetated sand flats, males occurred almost exclusively in seagrass, whereas the females occurred at equal densities in both habitats. Estimates of intake rates seemed greater for individuals foraging in seagrass (0.114–0.128 g dry mass (DM)·min–1) than on sand (0.064–0.087 g DM·min–1). Males appeared less well adapted for foraging on sand than in seagrass, and the presence of females could interfere with males' foraging on sand. The reason for the equal distribution of the females between the habitats probably lies in the inability of one particular habitat to support the entire population of females. Little interhabitat movement by individuals was detected. Within a habitat, sexes moved synchronously at the 1-ha scale but showed a degree of segregation at the individual scale, suggesting that some avoidance existed. Three diet types were observed in the godwits in the area: (1) dominated by soldier crabs (Mictyris longicarpus) on sand, (2) dominated by sentinel crabs (Macrophthalmus spp.) in seagrass, and (3) dominated by soft-bodied prey in seagrass.

Game-theoretical models have been highly influential in behavioural ecology. However, these models generally assume that animals choose their action before observing the behaviour of their opponents while, in many natural situations, individuals in fact continuously react to the actions of others. A negotiation process then takes place and this may fundamentally influence the individual attitudes and the tendency to cooperate. Here, I use the classical model system of vigilance behaviour to demonstrate the consequences of such behavioural negotiation among selfish individuals, by predicting patterns of vigilance in a pair of animals foraging under threat of predation. I show that the game played by the animals and the resulting vigilance strategies take radically different forms, according to the way predation risk is shared in the pair. In particular, if predators choose their target at random, the prey respond by displaying moderate vigilance and taking turns scanning. By contrast, if the individual that takes flight later in an attack endures a higher risk of being targeted, vigilance increases and there is always at least one sentinel in the pair. Finally, when lagging behind its companion in fleeing from an attacker becomes extremely risky, vigilance decreases again and the animals scan simultaneously.

<p><strong>Abstract.</strong> Land cover change is a dynamic phenomenon addressing environmental issues including natural calamities. Recent advancements in geospatial technology and availability of remote sensor data have fostered monitoring and mapping of land cover changes more precisely. Remote sensing is widely used where emerging research findings are focused mainly on coastal hazard studies. Tropical cyclones being an extreme weather event are more powerful and hazardous to southern parts of the Indian subcontinent. Aftermath of the cyclone is extreme causing land cover changes like defoliation, water logging, destruction of cultivable lands, plantations shrub vegetation, dissolving salt pans etc. The tropical cyclones are fierce to devastate the coastal districts of Tamil Nadu and make it a prey to these cyclones. In this paper, an attempt has been made to assess the pre and post cyclonic land cover change by utilizing potential microwave Synthetic Aperture Radar (SAR) dataset. The study portrays the occurrence of a severe cyclonic storm named <q>Gaja</q> that was formed over Bay of Bengal which hit Tamil Nadu on 15^th of November 2018 causing high death toll and demolition. The study focuses on the pre and post damage assessment provoked by Gaja cyclone. For analysis, a methodical procedure was followed by utilizing the Sentinel 1 SAR dataset. Random Forest (RF) classifier approach was incorporated for mapping land cover types as it reduces the variance among the classes thus yielding accurate predictions. Results demonstrate that classified imagery using dual polarization SAR dataset outperforms well for RF classifier thus escalating the overall accuracy.</p>

There is a paucity of information on the foraging ecology, especially individual use of sea-ice features and icebergs, over the non-breeding season in many seabird species. Using geolocators and stable isotopes, we defined the movements, distribution and diet of adult Antarctic petrels Thalassoica antarctica from the largest known breeding colony, the inland Svarthamaren, Antarctica. More specifically, we examined how sea-ice concentration and free-drifting icebergs affect the distribution of Antarctic petrels. After breeding, birds moved north to the marginal ice zone (MIZ) in the Weddell sector of the Southern Ocean, following its northward extension during freeze-up in April, and they wintered there in April–August. There, the birds stayed predominantly out of the water (60–80% of the time) suggesting they use icebergs as platforms to stand on and/or to rest. Feather δ 15 N values encompassed one full trophic level, indicating that birds fed on various proportions of crustaceans and fish/squid, most likely Antarctic krill Euphausia superba and the myctophid fish Electrona antarctica and/or the squid Psychroteuthis glacialis . Birds showed strong affinity for the open waters of the northern boundary of the MIZ, an important iceberg transit area, which offers roosting opportunities and rich prey fields. The strong association of Antarctic petrels with sea-ice cycle and icebergs suggests the species can serve, year-round, as a sentinel of environmental changes for this remote region.

Imprint cytology (touch prep) is often used for intraoperative examination of sentinel nodes in breast cancer. This allows axillary lymph node dissection (ALND) to be performed immediately for tumor-positive nodes. We evaluated the accuracy of touch prep examination of sentinel nodes and its role in the surgical treatment of breast cancer. We analyzed 169 breast cancer patients who underwent 170 lymphatic mapping procedures with intraoperative touch prep examination. Results from the touch prep were correlated with histopathology and clinical variables. There were 115 true-negative, 35 true-positive, 15 false-negative, and 5 false-positive results. Touch prep had a sensitivity of 70 per cent and specificity of 96 per cent. Positive predictive value, negative predictive value, and diagnostic accuracy were all 88 per cent. The false-negative rate was 30 per cent and correlated with the size of the nodal metastasis and number of involved nodes, but not other patient factors. Touch prep is useful for the evaluation of sentinel nodes in breast cancer, but it has a lower sensitivity than initially reported, particularly in patients with micrometastases. False positive results occur, although they may be reduced after experience with the technique. We recommend that suspicious findings on touch prep should be confirmed by frozen section and that ALND only be performed for histologically documented metastases. We currently perform touch prep only in patients who are at high risk of nodal metastasis or will undergo mastectomy. This improves operative efficiency and limits the impact of false positive and negative results without dramatically increasing the number of patients who require a second surgical procedure.

Undang-undang Cipta Kerja Omnibus law telah di sah kan, pengesahan tersebut mendapat penolakan dari berbagai elemen masyarakat. Hal itu disebabkan Omnibus Law UU Cipta Kerja, dinilai akan membawa dampak buruk bagi tenaga kerja atau buruh. Oleh karena itu peneliti melakukan analisis sentimen dalam bidang data mining terhadap UU Cipta Kerja Omnibus law pada media sosial Twitter. Penulis menggunakan metode Naïve Bayes dan KNN sebagai algoritma yang diterapkan dalam system berbasis java untuk memperbandingkan tingkat akurasi dari kedua metode tersebut. Peneliti menggunakan metode web scrapping untuk proses penggambilan data dari twitter secara real time. Hasil penelitian menunjukan bahwa analisis sentimen terhadap data Twitter terhadap UU Cipta Kerja Ombibus law dengan menggunakan metode Naïve Bayes mencapai tingkat akurasi 75% dengan class precision untuk pred. positive adalah 57%, pred negative adalah 72%, dan pred. neutral adalah 54%. Lalu pada metode KNN tingkat akurasi mencapai 88%. Dimana class precision untuk pred. positive adalah 94%, pred negative adalah 61%, dan pred. neutral adalah 58%.

BPJS is really helpful because one of its goal is to provide good service for the member in terms of healthiness. But, when there’s many people using the service, then it will cause more pros and contras. Therefore, researcher will be doing sentiment analysis in the field of data mining towards bpjs users on social media Twitter as much as 1000 data that later will be filtered to be 903 data because there are some data that has been duplicated. Researchers used the KNN, Decision Tree, and Naïve Bayes methods to compare the accuracy of the three methods. Researchers used the RapidMiner version 9.7.2 tools. The results showed that the sentiment analysis of Twitter data on BPJS services using the KNN method reached an accuracy level of 95.58% with class precision for pred. negative is 45.00%, pred. positive is 0.00%, and pred. neutral is 96.83%. Then the Decision Tree method the accuracy rate reaches 96.13% with the precision class for pred. negative is 55.00%, pred. positive is 0.00%, and pred. neutral is 97.28%. And the last one is the Naïve Bayes method which achieves 89.14% accuracy with precision class for pred. negative is 16.67%, pred. positive was 1.64%, and pred. neutral is 98.40%.

As virtual environments grow in complexity, size, and scope users will be increasingly challenged in assessing the situation in them. This will occur because of the difficulty in determining where to focus attention and in assimilating and assessing the information as it floods in. One technique for providing this type of assistance is to provide the user with a first-person, immersive, synthetic environment observation post, an observatory, that permits unobtrusive observation of the environment without interfering with the activity in the environment. However, for large, complex synthetic environments this type of support is not sufficient because the mere portrayal of raw, unanalyzed data about the objects in the virtual space can overwhelm the user with information. To address this problem, which exists in both real and virtual environments, we are investigating the forms of situation awareness assistance needed by users of large-scale virtual environments and the ways in which a virtual environment can be used to improve situation awareness of real-world environments. A technique that we have developed is to allow a user to place analysis modules throughout the virtual environment. Each module provides summary information concerning the importance of the activity in its portion of the virtual environment to the user. Our prototype system, called the Sentinel, is embedded within a virtual environment observatory and provides situation awareness assistance for users within a large virtual environment.

AbstractThe polar cod (Boreogadus saida) and the Antarctic silverfish (Pleuragramma antarctica) are pelagic fish endemic to the Arctic and Antarctica sea, respectively. Both species are abundant and play a central role as midtrophic wasp-waist species in polar ecosystems. Due to their biological and ecological characteristics (small size, complex life histories, relatively short generation cycles, movement capability, planktivorous diet, and importance as prey), the polar cod and the Antarctic silverfish are potentially good sentinels of ecosystem change. Changes in polar zooplankton communities are well documented. How changes impact ecosystems as a whole largely depend on the degree of diet specialization and feeding flexibility of midtrophic species. Here, we provide the ecomorphological characterization of polar cod and Antarctic silverfish feeding performances. A comparative functional ecology approach, based on the analysis of morpho-anatomical traits, including calculation of suction index and mechanical advantage in jaw closing, was applied to profile the feeding modes and flexibility of the two species. Ecomorphological evidence supports differences in food acquisition: the polar cod appears able to alternate particulate ram-suction feeding to a pump filter feeding, and the Antarctic silverfish results be both a particulate ram and a tow-net filter feeder. Both species exhibit opportunistic feeding strategies and appear able to switch feeding mode according to the abundance and size of the available prey, which is a clue of potential resilience to a changing environment.

Sentinel lymph node biopsy has become an accepted procedure for staging the axilla in early stage breast cancer. Our objectives were to review our practice of sentinel lymph node (SLN) mapping in breast cancer, to determine the impact of frozen section (FS) analysis of the SLN on patient management, and to compare our results to national data. We retrospectively reviewed the medical records of our patients with breast cancer who underwent SLN mapping with or without axillary lymph node dissection (ALND) between 1999 and 2006. During this period, 478 patients were treated for breast cancer, with 227 patients undergoing SLN mapping. The SLN was identified in 201 patients, with a positive SLN found in 52 patients (25.9%). There was a discrepancy between the intraoperative analysis (FS/touch prep) and final pathology in 20 patients (11.3%). Nineteen of those patients had a negative FS with positive final pathology. Six of these patients underwent completion ALND. One patient had a false-positive FS with a negative ALND. No axillary recurrences were observed. Eight patients (3.5%) developed postoperative complications. Our practice has been to use intraoperative evaluation of the SLN to reduce the number of patients requiring a secondary ALND. In our study, six patients returned to the operating room for a completion ALND. Our complication rate and axillary recurrence rates were similar to national data.

Inter-annual variations in the diets of seabirds are often a reflection of resource availability, with population dynamics and community structure implications. We investigated the trophic niche of Magellanic penguins Spheniscus magellanicus during the pre-molt stage in 6 years (2009 and 2013-2017) at Martillo Island, Beagle Channel, Argentina, using carbon and nitrogen stable isotope analysis of feathers. We found higher values in 2009 compared to the other years for both isotopes and estimated different proportions of prey in the diet of Magellanic penguins throughout the years. In 2009, penguins consumed mainly a mixture of benthic and benthopelagic fish, whereas from 2013 to 2017, the proportion of the pelagic form of squat lobster Munida gregaria, considered a key species in the Beagle Channel, increased over time and nearly dominated diets in 2017. Our results confirm that Magellanic penguins act as sentinels, reflecting shifts in the marine community of the Beagle Channel via inter-annual variation in their trophic niche and diet composition.

94 Background: Routine node evaluation involves simple bivalving the node with slides made from the resultant surfaces. SLNs are more thoroughly evaluated with 2-3 mm slices and histologic evaluation of each slice, resulting in many more slides to review. These slides would often have immunohistochemical analysis adding to the time and cost. In early studies of SLN efficacy and accuracy, the mean number of nodes was between 2 and 3 per patient though with some variation. As the number of submitted SLNs increases, the concern of benefit of the procedure comes into question. This is a retrospective examination of all SLN procedures for the year 2012 to examine the variation in the number of SLNs submitted for focused pathologic evaluation. Variation in the number of nodes submitted by surgeon is determined along with the associated costs of the pathologic evaluation. In addition, intraoperative evaluation of lymph node status may be useful to allow for immediate axillary node dissection during the initial operation. Utilization of this procedure was also examined to determine ultimate correlation with permanent pathologic evaluation. Methods: Retrospective examination of all breast cancer patients >=18 yrs who underwent SLN procedures during 2012. Introperative evaluation of lymph node for metastatic disease was also examined for correlation with permanent pathologic result. Results: 259 cases among 21 surgeons had touch prep performed. True positive number: 29. True negative number: 224. False negative number: 6. Fasle negative rate (FNR): 17.14%. Cost Effectiveness Evaluation: CPT 88307 - surg path gross and micro level V. CPT 88342 - Immunohistochemistry. CPT 88333 - Touch Prep. Pathologist time: 30 minutes per case. Conclusions: There is significant variation among surgeons as to number of cases and number of nodes submitted. There is a significantly greater number of sentinel lymph nodes examined by the pathologist as compared to the number of nodes submitted by the surgeon. The frequency of outliers is infrequent, but when it occurs there is significant cost in terms of time and dollars. The false negative rate from pathological evaluation is under study for isolated tumor cells.

SummaryBetween 1998 and 2011 we monitored the winter ranging behaviour of eight female Saker Falcons Falco cherrug fitted with satellite-received transmitters. Our tracking revealed that the winter home range area occupied by individual Saker Falcons varied greatly (median = 166 km2, range = 5-18,469 km2). A random forest model showed that Saker Falcons wintering on the Qinghai-Tibetan Plateau preferentially occupied areas with rich grassland (cover > 50%) on high altitude plateaus (4,000–5,000 m asl) with low levels of anthropogenic influence. Plant biomass in rich grasslands can support high winter densities of plateau pikas Ochotona curzoniae, which likely explains the preference exhibited by Saker Falcons for grassland cover > 50%. Factors influencing the abundance and distribution of this ‘keystone’ prey species are likely to have an effect on Saker Falcons and other predatory species. A key element of rangeland management on the Qinghai-Tibetan plateau has been the establishment of extensive protected areas as part of a strategy to balance economic and social development with the requirement of sustainably managing water resources, maintaining rangelands for pastoralists and conserving biological diversity. Wide ranging predatory species, such as the Saker Falcon, can be useful indicators of biodiversity in protected areas and act as ‘sentinels’ for anthropogenic changes that may impact many different taxa.

Mastectomy has long been a standard option for patients with ductal carcinoma in situ (DCIS). It is preferentially chosen by some women and may be suggested for individuals with recurrent, multifocal, or multicentric disease. We chose to evaluate our recent experience with mastectomy for DCIS. A retrospective review was conducted of 83 patients (87 breasts) from 1995 to 2006 who underwent mastectomy for DCIS. Mastectomy for DCIS was performed in 49 postmenopausal, 33 premenopausal, and one male patient. The average age was 53 years and the mean follow up was 4.5 years. Sentinel lymph node (SLN) biopsy was performed on 44 cases; positive nodes were identified in two. Intraoperative analyses of SLN were all negative. Only one patient had ipsilateral recurrence of the skin (1.1%). DCIS with microinvasion was noted in 32 per cent of the patients; none of these patients had ipsilateral recurrence. Three patients had positive microscopic margins; none have recurred to date. These results confirm the usefulness of mastectomy for patients with DCIS. We recommend SLN biopsy without intraoperative touch prep analysis. Additional treatment may not be required in patients with microinvasion, positive or close margins because our series shows no local recurrence in these patients.

The objective of this article is to describe the epidemiology of lymphogranuloma venereum (LGV) and non-LGV Chlamydia trachomatis anorectal infections in France and to examine the characteristics of the affected populations via a voluntary sentinel surveillance system for LGV between 2010 and 2015. Anorectal samples positive for C. trachomatis (CT) were sent by the participating laboratories to the National Reference Center for CT for LGV identification. Biological and clinical data were collected by biologists and clinicians. There were 1740 LGV episodes and 2248 non-LGV episodes. Continuous monitoring highlighted a sharp increase in the number of LGV and non-LGV anorectal infections, which were 2.3-fold and 6.5-fold, respectively. Most of the infections occurred in men who have sex with men. LGV patients were older than non-LGV patients and were more frequently human immunodeficiency virus (HIV)-positive compared to non-LGV patients. Anorectal LGV was significantly associated with residence in Paris, HIV co-infection, concurrent syphilis and bloody anal discharge. Undocumented patient characteristics were strongly associated with anorectal LGV. The anorectal LGV epidemic is poorly controlled in France. Early detection and prompt treatment of patients and their sexual partners are required to prevent transmission in the context of pre-exposure prophylaxis (PrEP) for HIV infection.

Habitat fragmentation threatens terrestrial arthropod biodiversity, and thereby also leads to alterations of ecosystem functioning and stability. Predation on insects and seeds by arthropods are two very important ecological functions because of their community-structuring effects. We addressed the effect of fragment connectivity, fragment size, and edge effect on insect and seed predation of arthropods. We studied 60 natural fragments of two grassland ecosystems in the same region (Hungarian Great Plain), 30 forest-steppes, and 30 burial mounds (kurgans). The size of fragments were in the range of 0.16–6.88 ha for forest-steppe and 0.01–0.44 ha for kurgan. We used 2400 sentinel arthropod preys (dummy caterpillars) and 4800 seeds in trays for the measurements. Attack marks on dummy caterpillars were used for predator identification and calculation of insect predation rates. In the case of seeds, predation rates were calculated as the number of missing or damaged seeds per total number of exposed seeds. Increasing connectivity played a role only in generally small kurgans, with a negative effect on insect and seed predation rates in the edges. In contrast, fragment size moderated edge effects on insect and seed predation rates in generally large forest-steppes. The difference between edges and centres was more pronounced in small than in large fragments. Our study emphasizes the important role of landscape and fragment-scale factors interacting with edge effect in shaping ecosystem functions in natural grassland fragments of modified landscapes. Managing functional landscapes to optimize the assessment of ecosystem functions and services needs a multispatial scale approach.

Over a period of five years (2005&ndash;2010) a total of 358 red fox (Vulpes vulpes) serum samples were collected during regular hunting procedures, mostly in the continental part of Croatia. All samples were tested using the microscopic agglutination test (MAT) for the presence of specific antibodies (Ab) to 12 Leptospira spp. pathogenic serovars. Specific antibodies for 11 different Leptospira spp. serovars (Australis, Sejroe, Icterohaemorrhagiae, Saxkoebing, Grippotyphosa, Tarassovi, Ballum, Pomona, Poi, Batavie, and Canicola) were detected in 121 red fox serum samples (33.8%). Among the positive samples antibodies for serovar Australis were found most frequently (32.1%), followed by Abs for serovar Sejroe (18.2%) and Icterohaemorrhagiae (13.2%). The highest Ab titre (1 : 3200) was recorded for serovar Australis and together with serovars Sejroe and Icterohaemorrhagiae this serovar showed a statistically significant frequency in cross-reactions (recorded in 23.1% cases). High Ab titres for serovars Grippotyphosa and Tarassovi (1 : 800) were detected for the first time in red foxes from the continental part of Croatia. The significant percentage (&asymp; 35%) of seropositive reactions to Leptospira spp. serovars implies the presence of pathogenic agents in the natural habitats of red foxes in Croatian low-land territories. The high Ab prevalence for specific serovars (Australis and Sejroe) detected in the fox population suggests predator-prey chain transmission of leptospirosis between red foxes and small terrestrial mammals, whilst the high Ab prevalence for serovar Icterohaemorrhagiae indicates an occasional contact of red foxes with rats. Red foxes can be thought of as susceptible hosts for serovars Australis and Sejroe and a possible link in leptospirosis transmission to other wild carnivores such as martens, weasels and ferrets. In contrast, this serologic survey revealed no evidence of serovar Canicola interchange between domestic carnivores (dogs) and red foxes. It can be concluded that red foxes are reliable sentinels for the epidemiological monitoring of leptospirosis, especially in lowland habitats.

When do Salps bloom? In this MBIE Curious Minds funded project we sought to address the growing need to involve people at a young age in learning involving active scientific research, to enhance societal understanding of science and technology and promote careers in STEM/STEAM to groups traditionally less represented. We engaged Leigh Primary School children with Salp research led by Dr Moira Decima from NIWA, and with citizen science through involvement in the co-design of a mobile app to report salp (marine invertebrate) sightings in coastal and underwater environments in our case study site: Goat Island Marine Reserve. The goal of the project was to engage students in cutting-edge marine science research and conservation; and in technological development through the co-design of a mobile app to report salps sightings in local coastal environments. Salps constitute essential prey items for multiple species of fish (including commercially important species like Hoki and Oreos), and can play a major role in ocean biogeochemistry by enhancing carbon (CO2) sequestration. In addition, the presence and extent of population blooms has increased in some parts of the world, presumably as a consequence of global warming, making these organisms sentinels of climate change. They are also unique in New Zealand because they seem to predictably bloom during the summer in coastal areas, yet this information is anecdotal and hence constituted a real opportunity to involve Leigh School to contribute to globally-relevant marine research. Important to local communities, the presence and abundance of these organisms affects tourist and local enjoyment of marine habitats, as low densities can result in attractive items for underwater experiences, but high densities render diving, fishing, boating and other water activities problematic. Students participated in a series of face-to-face events, including two visits to the Goat Island Marine Discovery Centre (University of Auckland); vlogging with Dr Decima while leading the RV Tangaroa on the #SalpPOOP (Salp Particle expOrt and Oceanic Production) research voyage; snorkelling at Goat Island Marine Reserve; and learning presentations by Leigh School children during SeaWeek 2019, where students presented their learning, enjoyed a sausage BBQ, and tried out virtual reality experiences designed to learn about salp research and marine conservation in general. We also engaged with Goat Island Dive & Snorkel dive instructors and selected customers during the app co-design phase to gain app prototype feedback. A mobile application (salpcount.nz) following a citizen science engagement framework that will allow data collection of salps in New Zealand was created with input from Leigh School students and selected Goat Island Dive & Snorkel customers and instructors, and with the engagement of multiple partners and through a hands-on educational program. Leigh School students were able to learn about an interesting marine biology topic relevant to them, while contributing to an application that will hopefully produce data through citizen science on the patterns and frequency of salp blooms around New Zealand. Here we report on this case study project highlighting what worked and what we learned, and some implications for future practice.

Abstract While acute lymphoblastic leukemia (ALL) is a prototype of cancer that can be cured by chemotherapy alone, current ALL treatment regimens rely primarily on conventional cytotoxic agents with significant acute and long-term side effects. Better understanding of genomic landscape of ALL is critical for developing molecularly targeted therapy and implementing genomics-based precision medicine in this cancer. In particularly, sentinel chromosomal translocations are common in ALL and often involve key transcription factors important for hematopoiesis. Epigenetic regulator genes are also frequently targeted by somatic genomic alterations such as sequence mutations (e.g., CREBBP) and gene fusions (e.g., MLL, EP300). To comprehensively define transcriptomic abnormalities in childhood ALL, we performed RNA-seq of an unselected cohort of 231 children enrolled on the MaSpore frontline ALL protocols in Singapore or Malaysia. In total, we identified 58 putatively functional and predominant fusion genes in 125 patients (54.1%), the majority of which have not been reported previously. In particular, we described a distinct ALL subtype with a characteristic gene expression signature driven by chromosomal rearrangements of the ZNF384 gene with different partners (i.e., histone acetyl-transferases EP300 and CREBBP, TAF15, and TCF3). In 9 of 11 ALL cases with ZNF384 rearrangements, the breakpoint in this gene was invariably between exon 2 and exon 3, resulting in deletion of the 5'-UTR and then in-frame fusion of the entire ZNF384 coding sequence with the partner genes. The top two most significantly up-regulated genes in the ZNF384-rearranged group were CLCF1 and BTLA, whose expression levels were 15.5- and 15.0-fold higher than in ALL cases with wildtype ZNF384, respectively. In fact, ZNF384 binding was identified within the CLCF1 and BTLA loci (particularly the promoter regions) by chromatin immunoprecipitation sequencing in B lymphoblasoid cells. Using luciferase transcription driven by CLCF1 promoter in HEK293T cells as a model system, we observed significantly greater transcription activity with EP300-ZNF384 fusion compared to cells expressing wildtype ZNF384, suggesting that this chimeric gene resulted in gain of ZNF384 function. Similar results were obtained with luciferase transcription assay driven by the BTLA promoter. In human ALL cells, CLCF1 and BTLA promoter activities were consistently and significantly higher in ZNF384-rearranged ALL than in ALL cell line with wildtype ZNF384. To examine the effects of ZNF384 fusion on hematopoietic stem and progenitor cell (HSPCs) function, we also evaluated colony forming potential of HSPC in vitro upon ectopic expression of ZNF384 fusions. While there was marked suppression of colonies from myeloid and erythoid lineages, expression of EP300-ZNF384 or CREBBP-ZNF384 significantly stimulated preB cell colony formation. However, neither EP300- nor CREBBP-ZNF384 fusion was able to transform mouse hematopoietic precursor cell Ba/f3 in vitro, but instead increased the transforming potential of other oncogenic mutations (NRASG12D). EP300-ZNF384 and CREBBP-ZNF384 fusion proteins lacked the histone acetyltransferase (HAT) domain, and showed only 25% and 10% of HAT activity of full-length EP300 and CREBBP, respectively, with dominant-negative effects. Also, expression of EP300-ZNF384 led to significant decrease in global H3 acetylation in Ba/f3 cells in vitro. Finally, in NRASG12D-transformed Ba/f3 cells, co-expression of EP300-ZNF384 or CREBBP-ZNF384 substantially potentiated cytotoxic effects of histone deacetylase inhibitor vorinostat. Similarly, in a panel of human ALL cell lines, ZNF384-rearrangement was also associated with increased sensitivity to vorinostat, suggesting that some ZNF384-rearranged ALL may benefit from therapeutic agents targeting histone acetylation regulation. In conclusion, our results indicate that gene fusion is the major class of genomic abnormalities in childhood ALL and chromosomal rearrangements involving EP300 and CREBBP may cause global epigenetic deregulation in ALL with potentials for therapeutic targeting. Disclosures No relevant conflicts of interest to declare.

Seven spot ladybird beetle, (Coccinella septempunctata) is a widely distributed natural enemy of soft-bodied insect pests especially aphids worldwide. Both the adult and larvae of this coccinellid beetle are voracious feeders and serve as a commercially available biological control agent around the globe. Different techniques are adopted to enhance the mass rearing and storage of this natural enemy by taking advantage of its natural ability to withstand under extremely low temperatures and entering diapause under unfavorable low temperature conditions. The key objective of this study was to develop a cost effective technique for enhancing the storage life and predatory potential of the larvae of C. septempunctata through cold storage in conjunction with the use of nuclear techniques, gamma radiations. Results showed that the host eating potential of larvae was enhanced as the cold storage duration was increased. Gamma irradiation further enhanced the feeding potential of larvae that were kept under cold storage. Different irradiation doses also affected the development time of C. septempuntata larvae significantly. Without cold storage, the lower radiation doses (10 and 25 GY) prolonged the developmental time as compared to un-irradiated larvae. Furthermore, the higher dose of radiation (50GY) increased the developmental time after removal from cold storage. This study first time paves the way to use radiation in conjunction with cold storage as an effective technique in implementation of different biological control approaches as a part of any IPM programs.Key wordGamma irradiations; cold storage, Coccinella septempunctata larvae; predatory potential; integrated pest management programme.INTRODUCTIONNuclear techniques such as gamma radiations have a vast application in different programmes of biological control including continuous supply of sterilized host and improved rearing techniques (Greany and Carpenter, 2000; Cai et al., 2017). Similarly irradiation can be used for sentinel-host eggs and larvae for monitoring survival and distribution of parasitoids (Jordão-paranhos et al., 2003; Hendrichs et al., 2009; Tunçbilek et al., 2009; Zapater et al., 2009; Van Lenteren, 2012). Also, at the production level, such technique may facilitate the management of host rearing, improve quality and expedite transport of product (Fatima et al., 2009; Hamed et al., 2009; Wang et al., 2009). Gamma irradiations can also be used to stop insect’s development to enhance host suitability for their use in different mass rearing programs (Celmer-Warda, 2004; Hendrichs et al., 2009; Seth et al., 2009). Development and survival of all insects have a direct connection with temperatures which in turn affect the physical, functional and behavioral adaptations (Ramløy, 2000). Many insects living in moderate regions can survive at low temperature by process of diapause. A temperature between 0 to 10oC may cause some insects to become sluggish and they only become active when the temperature is suitable. Such insects show greater adaptations to flexible temperature regimes for better survival. Many studies have reported this concept of cold-hardiness in insects in general (Bale, 2002; Danks, 2006) and specifically in coccinellid beetles over past years (Watanabe, 2002; Koch et al., 2004; Pervez and Omkar, 2006; Labrie et al., 2008; Berkvens et al., 2010). Using this cold hardiness phenomenon, many coccinellids have been studied for the effect of cold storage such as Coccinella undecimpunctata (Abdel‐Salam and Abdel‐Baky, 2000), Coleomegilla maculata (Gagné and Coderre, 2001) and Harmonia axyridis (Watanabe, 2002). This natural phenomenon, therefore, can be a helpful tool in developing low temperature stockpiling for improving mass-rearing procedures (Mousapour et al., 2014). It may provide a significant output in terms of providing natural enemies as and when required during pest infestation peaks (Venkatesan et al., 2000). Use of irradiation in conjunction with cold storage proves to be an effective technique in implementation of different biological control approaches as a part of any IPM programme. A study reported that the pupate of house fly, Musca domestica irradiated at dose of 500 Gy and can stored up to 2 months at 6°C for future use for a parasitoid wasp Spalangia endius rearing (Zapater et al., 2009). Similarly, when irradiated at 20 GY, parasitic wasps Cotesia flavipes were stored safely up to two months without deterioration of their parasitic potential (Fatima et al., 2009). Similarly, bio-control program of sugarcane shoot borer Chilo infescatellus proved successful through the use of irradiation combined with cold storage of its egg and larval parasitoids Trichogramma chilonis and C. flavipes (Fatima et al., 2009). Less mobile life stages such as larvae are of significance in any IPM strategy because they remain on target site for more time period as compared to adults. Therefore, use of predatory larvae is very promising in different biological control approaches because of their immediate attack on pests and more resistance to unfavorable environmental conditions than delicate egg stage. In addition, with their augmentation into fields, larval stage shows their presence for longer time than adult stage and their feeding potential is also satisfactory as that of adults. For the best utilization of these predators in the field and maximum impact of 3rd and 4th larval instars on prey, we should encourage late 2nd second instar larvae of predatory beetles in the fields as these instars have more feeding capacity due to increased size and ability to handle larger preys.In spite of higher significance, there is little information available about the effect of cold storage on the survival of larval instars of different ladybird beetles and its effect on their predatory potential. Very few studies report the use of cold storage for non-diapausing larval stage like for Semiadalia undecimnotata and only one study reported the short-term storage (up to two weeks) of 2nd and 3rd instar coccinellid, C. maculate, without any loss in feeding voracity of larvae after storage (Gagné and Coderre, 2001). The survival of 3rd and 4th larval instars of C. undecimpunctata for 7 days after storage at 5oC was reported in a study but the survival rate declined after 15-60 days of storage (Abdel‐Salam and Abdel‐Baky, 2000). As C. septempunctata is considered one of the voracious predators (Afroz, 2001; Jandial and Malik, 2006; Bilashini and Singh, 2009; Xia et al., 2018) and diapause is a prominent feature of this beetle and it may undergo facultative diapause under suitable laboratory conditions (Suleman, 2015). No information is available to date about the combined effect of cold storage and irradiation on the larval instars of this species.OBJECTIVES The objective of this study was to devise a cost effective technique for the cold storage and its effect on the subsequent predatory potential of the seven spotted ladybird beetle larvae in conjunction with the use of gamma radiations. Hypothesis of the study was that an optimum length of low temperature treatment for storage purpose would not affect the predation capacity of C. septempunctata larvae and their developmental parameters including survival and pupation will remain unaffected. Furthermore, use of gamma irradiation will have some additional effects on survival and feeding capacity of irradiated C. septempunctata larvae. Such techniques can be utilized in different biocontrol programs where short term storage is required. So these larvae can be successfully imparted in different IPM programs against sucking complex of insect pests as a component of biological control strategyMATERIALS AND METHODSPlant materials: Collection and rearing of C. septempunctata: Adult C. septempunctata were collected from the wheat crop (in NIAB vicinity and farm area) in the month of March during late winter and early in spring season 2016-2017. They were kept in plastic jars and were fed with brassica aphids. Under controlled laboratory conditions (25+2oC, 16h: 8h L:D and 65+5% R.H.), eggs of C. septempuctata were obtained and after hatching, larvae were also given brassica aphids as dietary source. Larvae of second instar were selected for this experiment (as the first instar is generally very weak and vulnerable to mortality under low temperatures). As the larvae approached second instar, they were separated for the experimentation. Irradiation of larvae at different doses: Irradiation of larvae was carried out by the irradiation source 137CS at Radiation laboratory, and the larvae were then brought back to the IPM laboratory, Plant Protection Division, Nuclear Institute for Agriculture and Biology (NIAB) Faisalabad. Radiation doses of 10 GY (Grey), 25 GY and 50 GY were used to treat the second instar larvae. There were three replicates for each treatment and five larvae per replicate were used. Control treatment was left un-irradiated.Cold storage of irradiated larvae: In present work, second instar C. septempunctata larvae were studied for storage at low temperature of 8oC. The larvae were kept at 8oC for 0, I and II weeks where week 0 depicts no cold treatment and this set of larvae was left under laboratory conditions for feeding and to complete their development. For larvae that were kept under cold storage for one week at 8°C, the term week I was devised. Similarly, week II denotes the larvae that remained under cold conditions (8°C) for two continuous weeks. Larvae were removed from cold storage in their respective week i.e., after week I and week II and were left under laboratory conditions to complete their development by feeding on aphids. Data collection: For recording the predatory potential of C. septempunctata larvae, 100 aphids were provided per larva per replicate on a daily basis until pupation as this number was more than their feeding capacity to make sure that they were not starved (personal observation). Observations were recorded for survival rate, developmental time and feeding potential. Data analysis: Data were statistically analysed by Statistical Software SPSS (Version 16.0). The data were subjected to normality check through the One-sample Kolmogorov-Smirnov test. Non normal data were transformed to normal data which were then used for all parametric variance tests. One-way and two-way analyses of variance were used. For comparison between variables, LSD test at α 0.05 was applied.RESULTSFeeding potential of irradiated larvae after removal from cold storage: Results showed an increase in the feeding potential of C. septempunctata larvae with increased cold storage duration. The feeding potential was significantly higher for the larvae that spent maximum length of time (week II) under cold storage conditions followed by week I and week 0. Gamma irradiations further enhanced the feeding potential of larvae that were kept under cold storage. When larvae were irradiated at 10 GY, the eating capacity of larvae increased significantly with the duration of cold storage. Similarly, larvae that were irradiated at 25 GY, showed increase in feeding potential on aphids as the time period of cold storage increased. The feeding potential of larvae that were irradiated at 50 GY, was again significantly increased with increase of cold storage duration. When different radiation doses were compared to week 0 of storage, there was a significant difference in feeding potential and larvae irradiated at 50 GY consumed the maximum numbers of aphids when no cold storage was done followed by larvae irradiated at 10 and 25 GY. With the other treatment, where larvae were kept under cold storage for one week (week I) the larvae irradiated at 50GY again showed the highest feeding potential. The feeding potential of irradiated larvae was again significantly higher than the un-irradiated larvae that were kept for two weeks (week II) under cold storage (table 1).Two-way ANOVA was performed to check the interaction between the different radiation doses and different lengths of storage durations for feeding potential of C. septempunctata larvae on aphids. The feeding potential of larvae irradiated at different doses and subjected to variable durations of cold storage were significantly different for both the radiation doses and cold storage intervals. Furthermore, the interaction between the radiation doses and storage duration was also significant meaning that the larvae irradiated at different doses with different length of cold storage were having significant variations in feeding levels (table 2).Developmental time of irradiated larvae after removal from cold storage: Significant difference was found in the development time of the larvae of C. septempunctata when irradiated at different doses at week 0 (without cold storage). The larvae irradiated at 10 GY took the maximum time for development and with the increase in irradiation dosage, from 25 to 50 GY, the time of development was shortened. The larvae irradiated at 50 GY had the same development time as the un-irradiated ones. When, the irradiated larvae were subjected to cold storage of one week duration (week I), their development time after removal from storage condition varied significantly. The larvae irradiated at 25 GY took the maximum time for development followed by larvae irradiated at 50 GY and 10 GY. There was an indication that the development time was extended for irradiated larvae as compared to un-irradiated larvae.Results also depicted a significant difference in the time taken by irradiated larvae to complete their development after taken out from cold storage of two weeks duration (week II). As the storage time of irradiated larvae increased, the development time was prolonged. Results showed that the larvae that were irradiated at 25 and 50 GY, took the maximum time to complete their development. With the prolonged duration of cold storage up to two weeks (week II), this difference of development time was less evident at lower doses (10 GY). The larvae irradiated at 10 GY showed a significant difference in their developmental duration after being taken out of cold storage conditions of the week 0, I and II. There was no difference in the developmental duration of larvae that were un-irradiated and subjected to different regimes of storage. Un-irradiated larvae were least affected by the duration of storage. With the increase in the storage time, a decrease in the developmental time was recorded. Larvae that were irradiated at 10 GY, took the maximum period to complete their development when no cold storage was done (week 0) followed by week I and II of cold storage. When the larvae irradiated at 25 GY were compared for their development time, there was again significant difference for week 0, I and II of storage duration. Maximum time was taken by the larvae for their complete development when removed from cold storage after one week (week I). With the increase in storage duration the time taken by larvae to complete their development after removal from cold storage reduced.When the larvae were removed after different lengths of cold storage duration i.e., week 0, week I and week II, there was a significant difference in the developmental time afterwards. Results have shown that the higher dose of radiation, increased the developmental time after removal from cold storage. The larvae irradiated at 50 GY took the longest time to complete their development after removal from cold storage (week I and week II) as compared the larvae that were not kept under cold storage conditions (week 0) (table 3).Interaction between the different radiation doses and different lengths of storage durations for development time of larvae were checked by two-way ANOVA. The development time of larvae irradiated at different doses and subjected to variable durations of cold storage were significantly different for both the doses and cold storage intervals. Furthermore, the interaction between the radiation doses and storage duration was also significant meaning that the larvae irradiated at different doses with different length of cold storage were having significant variations in development times (table 4). DISCUSSIONThe present research work indicates the possibility of keeping the larval instars of C. septempunctata under cold storage conditions of 8oC for a short duration of around 14 days without affecting its further development and feeding potential. Furthermore, irradiation can enhance the feeding potential and increase the development time of larval instars. This in turn could be a useful technique in mass rearing and field release programmes for biological control through larval instars. Usually temperature range of 8-10oC is an optimal selection of low temperature for storage as reported earlier for eggs two spotted ladybird beetle, Adalia bipunctata and the eggs of C. septempunctata (Hamalainen and Markkula, 1977), Trichogramma species (Jalali and Singh, 1992) and fairyfly, Gonatocerus ashmeadi (Hymenoptra; Mymaridae) (Leopold and Chen, 2007). However, a study reported more than 80% survival rate for the coccinellid beetle, Harmonia axyridis for up to 150 days at moderately low temperature of 3-6oC (Ruan et al., 2012). So there is great flexibility in coccinellid adults and larvae for tolerating low temperature conditions. After removal from cold storage, larvae showed better feeding potential with consumption of more aphids when compared to normal larvae that were not placed under low temperature conditions. This indicates that when the adult or immature insect stages are subjected to low temperature environment, they tend to reduce their metabolic activity for keeping them alive on the reserves of their body fats and sustain themselves for a substantial length of time under such cold environment. Hereafter, the larval instars that were in cold storage were behaving as if starved for a certain length of time and showed more hunger. This behavior of improved or higher feeding potential of stored larvae has been reported previously (Chapman, 1998). Hence, the feeding potential of C. septempunctata larvae significantly increased after cold storage. Gagné and Coderre (2001) reported higher predatory efficacy in larvae of C. maculata when stored at the same temperature as in the present study i.e., 8oC. Similarly, Ruan et al. (2012) showed that the multicolored Asian ladybug, H. axyridis, when stored under cold conditions, had more eating capacity towards aphids Aphis craccivora Koch than the individuals that were not stored. Such studies indicate that the higher feeding potential in insects after being subjected to low temperature environmental conditions could be due to the maintenance of their metabolism rate to a certain level while utilizing their energy reserves to the maximum extent (Watanabe, 2002).The individuals coming out from cold storage are therefore capable of consuming more pray as they were in a condition of starvation and they have to regain their energy loss through enhanced consumption. Furthermore, the starvation in C. septempunctata has previously been reported to affect their feeding potential (Suleman et al., 2017). In the present study, the larval development was delayed after returning to normal laboratory conditions. Cold storage affects the life cycle of many insects other than coccinellids. The cold storage of green bug aphid parasitoid, Lysiphlebus testaceipes Cresson (Hymenoptra; Braconidae) mummies increased the life cycle 3-4 times. Nevertheless, in current study the development process of stored larvae resumed quickly after taking them out and larvae completed their development up to adult stage. Similar kinds of results were reported for resumption of larval development after removal from cold storage conditions. Such studies only report satisfactory survival rates and development for a short duration of cold storage but as the length of storage is increased, it could become harmful to certain insects. Gagné and Coderre (2001) reported that cold storage for longer period (three weeks) proved fatal for almost 40% of larvae of C. maculata. Furthermore, in the same study, the feeding potential of C. maculata larvae was also affected beyond two weeks of cold storage due to the loss of mobility after a long storage period. Many studies have reported that longer durations of low temperature conditions can either damage the metabolic pathways of body cells or may increase the levels of toxins within the bodies of insects. Also, low temperature exposure for longer duration may cause specific interruptions in the insect body especially neuro-hormones responsible for insect development, which could be dangerous or even life threatening.Chen et al. (2004) also reported that the biological qualities of parasitized Bemisia tabaci pupae on population quality of Encarsia formosa were affected negatively with increase in cold storage duration. Similarly, the egg hatchability of green lacewing Chrysoperla carnea Stephen was lost completely beyond 18 days of cold storage (Sohail et al., 2019). However, in the present study the cold storage was done for maximum two weeks and it is to be regarded as a short term storage hence the survival rate was satisfactory. Longer periods of cold storage for larvae are not considered safe due to their vulnerable state as compared to adults which are hardier. Also 2nd instar larvae used in the present study for cold storage for being bigger in size and physical stronger than 1st instar. Abdel‐Salam and Abdel‐Baky (2000) reported that in C. undecimpunctata the cold storage of 3rd and 4th larval instars was higher and considered safer than early larval instars. The same study showed sharp decline in survival rate after two weeks and there was no survival beyond 30-60 days of cold storage. The present study showed that short term storage of the larvae of C. septempunctata could be done without any loss of their feeding potential or development so the quality of predator remained unaffected. Similar kind of work for many other insects had been reported previously where cold storage technique proved useful without deteriorating the fitness of stored insects. For example, the flight ability of reared codling moth Cydia pomonella Linnaeus remained unaffected after removal from cold storage (Matveev et al., 2017). Moreover, a sturdy reported that pupae of a parasitoid wasp Trichogramma nerudai (Hymenoptera; Trichogrammatidae) could be safely put in cold storage for above than 50 days (Tezze and Botto, 2004). Similarly, a technique of cold storage of non-diapausing eggs of black fly Simulium ornaturm Meigen was developed at 1oC. Another study reported safe storage of a predatory bug insidious flower bug Orius insidiosus for more than 10 days at 8°C (Bueno et al., 2014).In present study without cold storage, the lower doses of 10 and 25 GY prolonged the developmental time as compared to un-irradiated larvae and higher doses of irradiations in conjunction with cold storage again significantly prolonged the developmental time of larvae when returned to the laboratory conditions. Salem et al. (2014) also reported that Gamma irradiations significantly increased the duration of developmental stages (larvae and pupae) in cutworm, Agrotis ipsilon (Hufnagel). In another study, where endoparasitic wasps Glyptapanteles liparidis were evaluated with irradiated and non-irradiated gypsy moth Lymantria dispar larvae for oviposition, it was found that non-irradiated larvae had a shorter time to reach the adult stage as compared to irradiated larvae (Novotny et al., 2003). Both for higher doses with cold storage and lower doses without cold storage extended the larval duration of C. septempunctata. In another study when the parasitoid wasp Habrobracon hebetor was irradiated at the dose of 10 GY, it resulted in prolonged longevity (Genchev et al., 2008). In the same study, when another parasitoid Ventruria canescens was irradiated at lower doses of 4GY and 3 GY, it resulted in increased emergence from the host larvae, while gamma irradiations at the dose of 1 GY and 2 GY significantly stimulated the rate of parasitism (Genchev et al., 2008). The current study also indicated higher rates of predation in the form of increased feeding potential of larvae as a result of irradiations at lower doses.CONCLUSIONThe outcome of the current study shows that storage of 2nd instar C. septempunctata at low temperature of 8oC for a short duration of about 14 days is completely safe and could have broader application in different biocontrol programs. Such flexibility in storage duration can also assist in different mass rearing techniques and commercial uses. The combination of gamma radiation with low temperature cold storage could be a useful tool in developing different biological pest management programs against sucking insect pests. Incidence of periodic occurrence of both the target insect pests with their predatory ladybird beetles in synchrony is an important aspect that could be further strengthened by cold storage techniques. Therefore, short or long term bulk cold storage of useful commercial biocontrol agents and then reactivating them at appropriate time of pest infestation is a simple but an advantageous method in mass rearing programs. Increased feeding capacity of stored larvae is another edge and hence such larvae may prove more beneficial as compared to unstored larvae. Both cold storage and improved feeding of the C. septempuctata larvae can be utilized for implementation of IPM for many sucking insect pests of various crops, fruits and vegetables. Due to some constraints this study could not be continued beyond two weeks but for future directions, higher doses and longer duration periods could further elaborate the understanding and better application of such useful techniques in future IPM programmes on a wider scale. Also, some other predatory coccinellid beetle species can be tested with similar doses and cold storage treatments to see how effective this technique is on other species as well.ACKNOWLEDGMENTS We acknowledge the Sugarcane Research and Development Board for providing a research grant (No. SRDB/P/4/16) to carry out this research work. 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