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Oostingh, Gertie Janneke. "Human sensitisation to porcine transplantation antigens." Thesis, Open University, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272953.
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Patel, Manisha. "Singlet oxygen sensitisation in supercritical fluids." Thesis, Loughborough University, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.418373.
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Green, Brett James. "Detection and diagnosis of fungal allergic sensitisation." University of Sydney, 2005. http://hdl.handle.net/2123/978.
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Doctor of Philosophy(PhD),Airborne fungi are ubiquitous in the environment and human exposure is inevitable. Such fungi differ greatly in their taxonomic, physical, ecological and pathogenic characteristics. Currently, 69 000 species have been taxonomically classified and more than 80 of these are recognised to be aeroallergen sources. Many strategies have evolved to sample, identify and interpret fungal exposure to these species, however no strategy serves all purposes as exposure is a complex and dynamic process confounded by spatial, temporal and geographic variations in airborne counts, in addition to the inadequacies of the immunodiagnostic techniques available. To date, the interpretation of personal exposure and sensitisation to fungal allergens has been restricted to a few select species and the contribution of other genera, airborne hyphae and fragmented conidia to allergic disease are all poorly understood. The aim of the thesis was to utilize the Halogen Immunoassay (HIA) to diagnose fungal allergic sensitisation, to investigate the distribution and factors influencing allergens of fungi in the air and to understand what is actually inhaled in exposure settings. The novelty of the HIA derives from its unique ability to provide allergen sources that are actively secreted by the collected fungal spores and hyphae, which are bound to protein binding membranes (PBM) and then immunoprobed. In Chapter 2, the HIA was compared to the commercial in vitro Pharmacia UniCap assay (CAP) and the in vivo skin prick test (SPT), using 30 sera from subjects SPT positive to Aspergillus fumigatus and/or Alternaria alternata and 30 who were SPT negative to these fungi but sensitised to non-fungal allergens. Sera were analysed by CAP and the HIA against A. alternata, A. fumigatus, Cladosporium herbarum and Epicoccum purpurascens and compared statistically. Between 3% and 7% of SPT negative sera were identified to have specific IgE towards A. fumigatus and A. iv alternata, respectively. For the SPT positive sera, significant associations were found between the HIA and CAP scores for all fungal species tested (P<0.0001). Correlations between the HIA and SPT however, were weakly correlated for A. alternata (rs = 0.44, P<0.05) but not for A. fumigatus. In Chapter 3, personal exposure to indoor fungal aerosols was examined using the HIA to identify the fungal components that people were allergic to. Personal air sampling pumps (PASs) collected airborne fungal propagules onto PBMs for 2.5 hours indoors (n=21). Collected fungi were incubated overnight in a humid chamber to promote the germination of conidia. The membranes were then immunostained with pooled human Alternaria species-positive sera. All air samples contained fungal hyphae that expressed soluble allergens and were significantly higher in concentration than counts of conidia of individual well-characterised allergenic genera. Approximately 25% of all hyphae expressed detectable allergen compared to non-stained hyphae (P<0.05) and the resultant localisation of immunostaining was heterogeneous among hyphae. Fungal conidia of ten genera that were previously uncharacterised as allergen sources accounted for 8% of the total conidia that demonstrated IgE binding. In Chapter 4, the number and identity of fungi inhaled by 34 adults in an outdoor community setting was measured over 2 hour periods by people wearing Intra-nasal air samplers (INASs) and compared to fungal counts made with a Burkard spore trap and filter air samplers worn on the lapel. Using INAS, the most prevalent fungi inhaled belonged to soil borne spores of Alternaria, Arthrinium, Bipolaris, Cladosporium, Curvularia, Epicoccum, Exserohilum, Fusarium, Pithomyces, Spegazzinia, Tetraploa and Xylariaceae species, in addition to hyphal fragments. These results showed that inhaled exposure in most people varied in a 2-fold range with 10-fold outliers. In addition, the INAS and personal air filters agreed more with each other than with Burkard spore trap counts. The analysis was further confounded by different sampling efficiencies, locations of devices and ability to visualise and count fungal propagules. In Chapter 5, a double immunostaining technique based on the HIA was developed and applied to the conidia, hyphae and fungal fragments of A. alternata, A. fumigatus and Penicillium chrysogenum to discriminate between sources of allergens, v using IgE and to identify the fungi, using a fungal-specific antibody. The localisation of immunostaining was heterogeneous between both conidia and the state of germination with greater concentrations of double immunostaining detected following germination for each fungal species (P<0.0001). Fragmented A. alternata hyphae and morphologically indiscernible fragments could be identified for the first time using this technique. In Chapter 6, the factors affecting the release of allergen from the spores of eleven different species were studied. For nine of eleven species, between 5.7% and 92% of spores released allergen before germination. Ungerminated spores of P. chrysogenum and Trichoderma viride did not release detectable allergen. After germination, all spores that germinated eluted allergen from their hyphae. Upon germination there was a significant increase in the percentage of spores eluting detectable allergen (P<0.0001) and the localisation of allergen along the hyphae varied between species. Increased elution of allergen post germination might be a common feature of many species of allergenic fungi following inhalation. Additionally, Chapter 6 explored the extent to which inhaled spores or hyphae germinate after deposition in the nasal cavity and thus cause exposure to allergens. Twenty subjects had their noses lavaged at three separate intervals, (1) at the beginning of the experiment, (2) after one hour indoors and (3) after one hour outdoors. The recovery of spores and hyphal fragments from the nasal cavity varied between individuals and was significantly greater after outdoor exposures. Germinated fungal spores were recovered often in high concentrations for Aspergillus-Penicillium species, however the proportion between ungerminated and germinated spores were much lower for other genera recovered. Conclusions: Our analysis of cultured and wild-type fungi presents a new paradigm of natural fungal exposure, which in addition to commonly recognized species, implicates airborne hyphae, fragmented conidia and the conidia of a much more diverse range of genera as airborne allergens. Exposure is heterogeneous between individuals in the same geographic locality and the spectrum of fungal genera inhaled differs with the method of analysis. Many of the spores inhaled are likely to be allergenic, however upon germination there is an increased elution of allergen and this might be a common vi feature of many fungal species following inhalation. This project also provides novel techniques to diagnose fungal allergy by immunostaining wild-type fungi to which a patient is exposed with the patient’s own serum. Such an immunoassay combines environmental with serological monitoring on a patient specific basis and potentially avoids many problems associated with extract variability, based on the performance of current diagnostic techniques for fungal allergy.
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Wildman, Scott Shaw. "Purinergic signalling : sensitisation of recombinant P2X receptors." Thesis, University College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325339.
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Nilsson, Caroline. "Cytokine profiles, infections and IgE sensitisation in childhood /." Stockholm : Department of Clinical Science and Education, Södersjukhuset : Sachs' Children's Hospital : Karolinska institutet, 2006. http://diss.kib.ki.se/2006/91-7140-720-0/.
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Davies, Catherine L. "Intermolecular sensitisation of Lanthanide Luminescence in Amphiphilic Systems." Thesis, University of York, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.520025.
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De, Silva Mahappuque Anushika Sumali. "Ca²⁺ sensitisation and desensitisation mechanisms in pulmonary artery." Thesis, King's College London (University of London), 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499978.
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Modulation of Ca²⁺sensitivity of the smooth muscle contractile apparatus plays a fundamental role in the regulation of force. Increased CA²⁺ sensitivity may contribute towards various disease conditions such as pulmonary hypertension. Ca²⁺ sensitivity is regulated by the balance between myosin light chain kinase (MLCK)-dependent phosphorylation and myosin phosphatase (MP)-dependent dephosphorylation of myosin light chain (MLC-20). Several protein kinases have been implicated in modulating this process. In particular RhoA and its effector Rho kinase (Rhok) inhibits MP activity via the MYPTl subunit; whereas protein kinase C (PKC) activates MP inhibitory protein CPI-17. Additionally the mitogen activated protein kinases (MAPK) and heat shock protein 27 (HSP27) may effectively modulate Ca²⁺ sensitivity via the cytoskeleton.
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Wright, Phillip James. "Sensitisation of lanthanide luminescence by rhenium tetrazolato complexes." Thesis, Curtin University, 2016. http://hdl.handle.net/20.500.11937/1135.
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Luminescent inorganic compounds have received a great deal of attention due to the ability to modulate their properties and obtain highly emissive compounds for applications in light emitting devices and cellular labelling. This study targets the formation of emissive, multinuclear, tetrazolato complexes of Re(I), and utilises these complexes as sensitisers for lanthanide luminescence. The results increase the understanding of energy transfer mechanisms in d-f metal complexes, and highlight an interesting area for potential investigation.
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Boziaris, Ioannis S. "Bacterial injury and sensitisation of gram-negatives to nisin." Thesis, University of Surrey, 2000. http://epubs.surrey.ac.uk/842954/.
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Nisin is a bacteriocin produced by Lactococcus lactis subsp. lactis, which is active against Gram-positive organisms including bacterial spores. It is not generally active against Gram-negative bacteria, yeasts and fungi. Gram negatives show nisin-sensitivity when their outer membrane permeability is altered by various means, such as treatments with chelators, e.g. EDTA, osmotic shock, heating, freezing, freeze-drying, high- pressure etc. Application of chelators and nisin is effective against Gram-negatives when exogenous nisin is added. Nisin produced in situ and chelators are not an effective combination, since nisin production follows the pH drop caused by sugar fermentation, and this interferes with the sequestering ability of the chelators. Presence of nisin during thermal inactivation of Gram-negatives though is effective. Bacteria become structurally injured during heating showing sensitivity to agents like SDS and deoxycholate and extended detection times by impedimetry. These injured bacteria are inactivated by nisin, with a concomitant reduction of the measured D-values. Low pH and the presence of small amount of chelators enhance the injury and inactivation and reduce D-values further. Gram-negative bacteria injured by chilling and freezing are also sensitive to nisin. The effectiveness of nisin is reduced in a food environment mostly of nisin binding to fat, and food particles. D-values were decreased less or not at all in egg white and liquid whole egg, respectively, and rapid chilling of bacteria attached to chicken skin in presence of nisin did not give the effect seen in laboratory media. Nisin is active against heat-, chill-, and freezing-stressed Gram-negatives only if it is present during the treatments. When the stress factor is removed, the bacteria recover their nisin resistance, implying transient susceptibility to nisin, but not to smaller molecules. This is probably due to rapid reorganisation and restoration of OM permeability damage, rather than biochemical repair. The LPS chain length influences the sensitisation of Gram-negatives to nisin, only in the case of freezing, where the strain with the shorter LPS chain was more sensitive than the wild type. Heat-, and freezing-stressed bacteria lost lipopolysaccharides and increased their cell surface hydrophobicity. This was not seen with chill-stressed bacteria, which were sensitive to nisin though. This indicates that release of LPS is not a prerequisite for nisin sensitivity in Gram-negatives.
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Hamlin, Adam Scott. "Functional Neuroanatomy of Morphine-Induced Abstinence, Tolerance, and Sensitisation." University of Sydney, 2006. http://hdl.handle.net/2123/1164.
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Doctor of Philosophy (PhD)The investigation into the relationship between neural plasticity in the rat forebrain associated with opiate-induced behaviours yielded two major results. The major finding of the functional neuroanatomy of acute morphine dependence was that doses of naloxone that induced hyperalgesia following a brief exposure to morphine, in previously drug-naïve rats, caused a specific induction of the inducible transcription factor (itf) proteins c-Fos and zif268 in the extended amygdala. Moreover, doses of naloxone that caused a simple reversal in morphine analgesia failed to induce itf proteins in these same brain regions. This increase in itf proteins was specific to regions of the extended amygdala that receive and process nociceptive information relayed via the spino-parabrachio-amygdaloid pathway and was not observed in other regions that are involved in supraspinal pain modulation such as the rostral ventromedial medulla and the periaqueductal gray. We also found that acute morphine increased c-Fos protein in the basolateral amygdala and the major output nucleus of the central amygdala the medial subdivision. Acute morphine also up-regulated c-Fos protein in striatal, midbrain, and hypothalamic nuclei. A unique finding of the current study was that prolonged exposure to morphine was required to induce c-Fos in these brain regions, as the subsequent administration of naloxone 30-minutes after morphine either reversed or blocked this induction. These results indicate the potential role of the amygdala in analgesia following systemic morphine and in pain facilitation during acute morphine abstinence. Investigation into the neurons and circuitry that undergo long-term neuroplasticity in response to repeated morphine exposure revealed that network-level changes in the distribution of Fos protein in the nucleus accumbens and striatum predicted both tolerance to catalepsy and psychomotor sensitisation. Drug-naïve rats became profoundly cataleptic following morphine, an effect that rats with a drug-history became tolerant. Rats with a history of morphine exposure showed an increase in stereotyped behaviours compared to drug-naïve rats. The major finding of this study was that a shift in the induction of c-Fos protein from a matrix predominance in drug-naïve rats toward a patch predominance in drug-sensitised rats in the accumbens core predicted both tolerance to catalepsy and sensitisation of oral stereotyped behaviours. Acute injection of morphine in a drug-naïve rat induced catalepsy and increased the number of c-Fos-positive neurons in matrix striatopallidal projection neurons of the rostral accumbens core. An increase in activity of striatopallidal projection neurons, which give rise to the indirect pathway, could potentially increase inhibitory drive to the pedunculopontine nucleus (PPN). The PPN, long known as a site of termination for basal ganglia output, is thought to direct the outflow of incentive-motivational and sensorimotor information from the nucleus accumbens to pons, medullary, and spinal cord nuclei translating the incentive impact of the stimuli into appropriate motor, autonomic and emotive responses (Winn et al., 1997). Inhibition of this nucleus would cause the animal to be unable to initiate a movement and in effect lock up, which is precisely what cataleptic postures look like. In contrast c-Fos-positive neurons were decreased in the rostral matrix and increased in patch striatonigral projection neurons along the rostro-caudal extent of the accumbens core when morphine was administered to drug-sensitised rats. Striatonigral neurons located in the patch give rise to the direct pathway innervating the dopaminergic neurons in both substantia pars compacta and the dopamine rich islands in the substantia nigra pars reticulata (Berendse et al., 1992; Gerfen, 1992; Furuta et al., 2002). Activity of this pathway is thought to be involved in the initiation of movement (Gerfen, 1992; Gerfen and Wilson, 1996), however, when this pathway is overstimulated as is the case when morphine is injected in drug-sensitised rats this could potentially cause increased activity of PPN neurons leading to repetitive psychomotor behaviours or stereotypy. This data adds to the growing body of evidence that suggests that long-term neuroadaptations induced by drugs of abuse including morphine that lead to behavioural sensitisation involves the circuitry that includes the nucleus accumbens.
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Divkovic, Maja. "Probing skin sensitisation mechanisms using in vitro proteomics techniques." Thesis, Imperial College London, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.428574.
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McFie, Lauren. "Incentive-sensitisation : the effects of drugs, stress and reward." Thesis, Goldsmiths College (University of London), 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.418023.
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Dipp, Michelle. "The role of calcium sensitisation in hypoxic pulmonary vasoconstriction." Thesis, University of Oxford, 2001. http://ora.ox.ac.uk/objects/uuid:d14ca3ef-c5b8-4a4c-b9d4-5a1ee086cb4a.
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Coward, Sam M. "The role of leukotrienes in murine sensitisation to aeroallergens." Thesis, University of Sunderland, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.402696.
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Bolton, Sally. "Sensitisation of the trigeminovascular system : implications for migraine pathophysiology." Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1445374/.
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Migraine is an episodic brain disorder characterised often by attacks of throbbing head pain and in many patients by sensitisation of the facial skin. Current research indicates that the dural blood vessels and their neural connectivity with the trigeminocervical complex play a fundamental role in the perception of these symptoms. As such, characterisation of this pathway may provide an insight into the pathological processes occurring during a migraine attack and offer new targets for the development of novel anti-migraine treatment strategies. Utilising the techniques of in vivo electrophysiology, intravital microscopy and c-fos immunohistochemistry, the studies in this thesis have explored the consequences of trigeminal primary afferent neuronal activation during the following conditions: Exposure to glyceryl trinitrate, a nitric oxide releasing compound, known to induce migraine in susceptible individuals. Peripheral sensitisation following application of various mediators to the dural and facial receptive field. Following "wind-up" stimuli, known to hyper-excite spinal neurons The findings from these studies demonstrate that the behaviour of second order neurons in the spinal trigeminal nucleus is fundamentally different following input from the dura mater compared to input from facial cutaneous afferents. Furthermore, the time course of sensitisation seen following application of prostaglandin E2 to the dural receptive field correlates well to the allodynia and hyperalgesia reported during migraine. Sensitisation of dural afferents may thus underlie at least part of the pathophysiology associated with migraine.
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Tracey, Sandra Michelle. "Dye sensitisation of sol-gel derived titanium dioxide films." Thesis, Sheffield Hallam University, 1997. http://shura.shu.ac.uk/20448/.
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This thesis describes the development and characterisation of dye sensitised Inorganic:Organic (10) heterojunction photovoltaic (PV) cells of the form Au/MPc/TiO2/InSnO2 or F-SnO2 (where MPc= copper phthalocyanine, chloroaluminium phthalocyanine or lead phthalocyanine). The transparent TiO2 films were prepared by Sol-Gel techniques and characterised optically, structurally and electrically. The effects of and interactions between Sol-Gel process parameters have shown that the parameters undergo significant interaction with particular effects on the TiO2 film thickness and thickness related properties obtained during dip coating. The film refractive index n was in the range 2.73-1.81 and wavelength dependent. The conductivity of the films derived from Au/TiO2/InSnO2 structure was 4.26x10[-6]S/cm. E[0] varied from 3.4-3.35eV where delta=2 indicting an indirect allowed transition in 1 layer thick films to E[0] ~3.2eV where delta=3 indicating indirect forbidden transition as the number of coating layers increased. The spectral response, dark and illuminated J(V) and dark C(V) characteristics of the dye sensitised 10 PV cells have been determined. Spectral response indicates that all of the organic dyes studied can be used to sensitise Sol-Gel derived TiO2 into the visible region Corresponding photovoltaic and junction parameters were derived. Photovoltaic effects were observed in all devices studied, however cell efficiencies were poor, in the range (eta~0.0001-0.046%). The low quantum efficiencies were anticipated to be a consequence of the presence of recombination centres at the TiO2/MPc heterointerfaces and the high observed series resistance due to the low conductivity of the MPc films. The MPc/TiO2 junction formed a rectifying contact. C(V) analysis indicated that the junction was electrically abrupt. The dark J(V) characteristics were divided into three regimes, (i) A reverse bias regime in which the device acts as a p-n heterojunction, (ii) an intermediate forward voltage regime, where the derived values of m > 2 indicating the presence of a high density of interface states (iii) High forward regime. All devices deviate from the standard diode equation as a consequence of space charge effects in the organic layer, the ideality factors m"2. Temperature dependence measurements of PbPc/TiO2 heterojunctions show that the junction currents are a composite of tunnelling and recombination. PV cell parameters were influenced by changes in ambient conditions, the thickness of organic layers and variations in incident intensity. The J[sc] and V[oc] were proportionally and logarithmically dependent on the incident intensity respectively. The high R[s] is believed to be responsible for the poor cell efficiencies reducing FF, J[sc] and eta. A 30 fold increase in eta was observed when the organic film thickness was reduced from 500nm to 100nm. Exposure to atmosphere reduced eta and is likely to be a consequence of O2 trapping centres. The work has demonstrated the feasibility of a low cost solid state 10 heterojunction photovolatic cell. However improvements in efficiency are required to produce a commercially viable device.
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Papenfuss, Kerstin. "Sensitisation to TRAIL-induced apoptosis by targeted inhibition of kinases." Thesis, Imperial College London, 2010. http://hdl.handle.net/10044/1/9014.
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The tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in cancer cell lines but not in normal cells. This property of TRAIL led to its development as a novel cancer drug. However, most primary tumour cells are TRAIL-resistant, yet, they can be sensitised by combining TRAIL with other cancer drugs. Kinase inhibitors have emerged as a new class of cancer drugs with high therapeutic potential and cancer cell specificity. The aim of this thesis was to determine the mechanism of TRAIL apoptosis sensitisation by inhibition of certain kinases that are specifically and aberrantly activated in cancer cells. When studying the TRAIL-induced phosphorylation of Bid it was discovered in this thesis that this phosphorylation was independent of ATM which has previously been described to phosphorylate Bid at this specific site. Remarkably, the ATM inhibitor KU-55933 used in this context was able to further sensitise HeLa cells to TRAIL-induced apoptosis and could break TRAIL resistance of the colon carcinoma cell line DLD1. As the combination of TRAIL and KU-55933 might represent a promising treatment option for cancer therapy this study focused on investigating the molecular mechanism that leads to TRAIL sensitisation by KU-55933. Surprisingly, TRAIL sensitisation by KU-55933 was independent of specific inhibition of ATM and, instead, achieved by inhibition of the phosphoinositide 3-kinase (PI3K) p110α isoform. Aberrant activation of PI3K α is a frequent tumour-specific alteration in various types of cancer including breast and colon carcinoma. It could be demonstrated that TRAIL apoptosis sensitisation of TRAIL-resistant DLD1 colon carcinoma cells by KU-55933 or PIK75, a specific inhibitor for p110α, required concomitant down-regulation of the cellular FLICE-inhibitory protein (cFLIP) and the X-linked Inhibitor of Apoptosis Protein (XIAP). Whilst suppression of cFLIP enhanced caspase-8 activation at the TRAIL death-inducing signalling complex (DISC), resulting in first cleavage of caspase-3, loss of XIAP enabled further cleavage and full activation of caspase-3. These results suggest that the combination of TRAIL or other TRAIL receptor agonists with inhibitors of PI3Kα may be an effective new strategy in cancer treatment capable of overcoming therapy resistance.
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Greeff, Mary Louise. "The influence of welding parameters on the sensitisation behaviour of 3CR12." Diss., Pretoria : [s.n.], 2006. http://upetd.up.ac.za/thesis/available/etd-04052007-124929.
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Jones, Amanda Clare. "Maternal and fetal immune responses during pregancy and the first year of life and the development of allergic disease." Thesis, University of Southampton, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242205.
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Zambreanu, Laura. "Central sensitisation and its relevance to chronic pain : FMRI studies in humans." Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.427610.
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McGregor, Pauline Lynda. "Investigation of the photodegradation of organic pollutants in water by dye sensitisation." Thesis, Edinburgh Napier University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.295375.
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Strid, Karin Jessica. "Immunoregulation after intestinal and cutaneous exposure to food proteins : tolerance versus sensitisation." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.407127.
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Afuwape, Adeyemi Olutosin. "Oral tolerance and sensitisation : immunoregulation after feeding of ovalbumin and cow's milk." Thesis, University College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312961.
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Lee, Michael Chin Han. "Identifying the neural correlates of central sensitisation and cannabinoid analgesia in humans." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608410.
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Lawler, Danielle Suzanne. "The role of respiratory viral infection and extracellular DNA in allergic sensitisation." Thesis, Lawler, Danielle Suzanne (2018) The role of respiratory viral infection and extracellular DNA in allergic sensitisation. Honours thesis, Murdoch University, 2018. https://researchrepository.murdoch.edu.au/id/eprint/43144/.
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The correlation between allergic disease and viral infections has been well established. While respiratory viral infections are strongly correlated with the development of allergic asthma, it is not known precisely how viral infection may produce, or alternatively protect, against allergic disease. Furthermore, little is known about the impact of viral infection on IgE sensitisation, due to conflicting and limited research. Recently, neutrophil extracellular traps (NETs) have been observed in viral infections and shown to induce a Th2 response. We aim to explore the link between virus infection and allergy and test if the link is mediated through NETs. We hypothesise that NETs are induced during viral respiratory infections, but that they do not adequately control the viral disease. Instead, they contribute to the development of allergic disease, and their removal will be beneficial to patients.
This hypothesis was addressed by sensitising rats to ovalbumin (OVA), during a respiratory viral infection and re-exposing the animals to OVA one week after sensitisation. To test the involvement of NETs, DNase-I was administered during viral infection to degrade NETs. OVA-specific IgE serum levels, along with cellular infiltrates into the airways, were compared between groups to assess allergic sensitisation before and after OVA re-exposure.
Although no effect on IgE sensitisation was observed with viral infection, DNase treatment reduced the risk of IgE sensitisation and increased T regulatory cell (Treg) proportions in the airways, which also displayed higher levels of FoxP3. We did not observe a difference between any of the groups in respect to allergic recall response suggesting that the achieved sensitisation was not sufficient to induce clinical disease. Our findings nevertheless suggest that DNase treatment induces a regulatory response, which may protect against allergic disease. Future studies should explore this immunoregulatory response, as a novel strategy for allergy protection.
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Benson, Christopher. "Characterising uncertainty and sensitisation in the response of MOSFET devices to mixed fields." Thesis, Lancaster University, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.421607.
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Propper, David J. "The influence of immunosuppressive agents and pregnancy on sensitisation to major histocompatibility antigens." Thesis, University of Aberdeen, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358128.
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The studies in this thesis concern alloantibody responses against MHC class I antigens. These antibodies are induced by blood transfusions and strongly associated with clinical kidney allograft rejection. These findings were: 1. Cytotoxic antibodies in 11 multiparous patients, who had become broadly sensitised by blood transfusions, were of IgG class and directed to HLA class I antigens. In all patients, some of these antibodies were directed against class I specificities expressed by the partner by whom the patient was parous. 2. In high responder multiparous rats, humoral responses against paternal MHC class I antigens reencountered in blood transfusions given after pregnancy were suppressed. In contrast, minor histocompatibility antigens shared between the paternal and later blood donor strains enhanced anti-MHC class I antibody responses. 3. In multiparous rats, anti-paternal antibodies stimulated by pregnancy were not influenced by materno-paternal disparities at MHC class II or minor antigenic loci, and were directed to conventional MHC class I epitopes. 4. In high responder rats, there was no evidence for either humoral or cellular tolerance to non-inherited maternal MHC antigens. 5. In high responder rats pre-treated with blood transfusions and concomitant cyclosporin A, subsequent antibody responses to third party MHC class I antigens encountered in challenge transfusions, given without cyclosporin A, were suppressed only when minor histocompatibility antigens were shared between the initial and challenge transfusions. 6. Three drugs:- cyclosporin A, FK506 and rapamycin abrogated anti-MHC class I alloantibody responses to blood transfusions by naive rats and, at the same time, induced humoral tolerance. None of these drugs, however, inhibited either ongoing, alloantibody synthesis or humoral anamnestic responses in high responder rats with established humoral immunity.
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Hussein, Athar Naser. "The mechanisms of plasticity in mesolimbic DA function underlying behavioural and neurochemical sensitisation." Thesis, University of Leicester, 2018. http://hdl.handle.net/2381/43031.
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Background: Repeated psychostimulant exposure results in progressive and enduring behavioural sensitisation modelling increased drug craving observed in human psychostimulant abusers. Aims: This project aimed to evaluate mechanisms underlying sensitisation-related neuroadaptations during repeated nicotine or amphetamine administration, and to investigate whether behavioural sensitisation to each drug employed the same or different processes. Methods: Behavioural sensitisation during repeated daily nicotine (0.6 mg/kg) or amphetamine (1 mg/kg) administration was measured, compared to saline control (1 ml/kg). Male Lister-hooded rats received five daily injections of drug or saline and locomotor activity was recorded. Ten days later, rats were challenged with the same doses of drug/saline and their locomotor activity was measured. Using immunohistochemistry, we measure expression of immediate early genes (IEG), activity-regulated cytoskeleton-associated protein (Arc) and c-fos, and of Methy1-CpG-binding-protein 2 (MeCp2) and brain-derived neurotrophic factor (BDNF), in nucleus accumbens (NAc) sub-regions, and ventral tegmental area (VTA). Finally, effects of drug pre-treatment on dopamine receptor gene expression in NAc and VTA, and on dopamine release in NAc shell and core subregions were investigated. Results: Nicotine and amphetamine increased locomotor activity, during daily treatment and challenge ten days later. Behavioural sensitization, was accompanied by increased in Arc and MeCp2 expression in NAc, which differed between nicotine and amphetamine treatment, but no changes in either c-fos, or BDNF were observed. Although there was evidence of behavioural and immunohistochemistry cross-sensitisation from amphetamine to nicotine, no evidence for either behavioural or immunohistochemistry cross-sensitisation from nicotine to amphetamine was seen. Dopamine receptor gene expression increased in VTA after nicotine pre-treatment and NAc after amphetamine pre-treatment, but treatment history did not affect dopamine release in NAc shell or core. Conclusions: Behavioural sensitization was accompanied by increases in IEG, MeCp2, and dopamine receptors gene expression, which differed between nicotine and amphetamine suggesting that sensitisation to the two drugs occurs through separate mechanisms, perhaps involving increased neuronal plasticity within different sub-regions of NAc and VTA.
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Ndhlovu, Nomathamsanqa. "The relationship between immunization and food allergy and sensitisation in South African children." Master's thesis, University of Cape Town, 2017. http://hdl.handle.net/11427/25420.
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Abstract
The prevalence of food allergies is higher in children compared to adults and it is increasing. The factors that influence food allergies in children are not clear. In light of the hygiene hypothesis, vaccinations may contribute towards to a predominant allergen specific response or exposure to the virus or microbe in the vaccine may decrease the risk for allergy. Previous studies have shown that the effect of vaccinations on food allergy and food sensitisation varies. Therefore, the aim of this study is to determine if a relationship exists between vaccinations and food allergies and food sensitisation in children in the first 18 months of life who live in urban Cape Town and in rural Mqanduli in the Eastern Cape. Secondary data analysis of an observational cross sectional study was carried out which involved univariate logistic regression to calculate odds ratios between self-reported immunisation status and food sensitisation and food allergy at a 95% confidence interval in children between 12 and 36 months of age. The same method was employed to investigate the relationship between immunisation and atopy. Multivariate analysis was utilised to adjust for potential confounders. Food sensitisation and food allergy were determined through skin prick tests (SPT) and oral food challenges respectively. The results indicate that, the number of participants positive for food sensitisation and allergy, eczema, hay fever and asthma were significantly greater in the urban sample (n= 708) compared to the rural sample (n= 400) (P<0.05). Further, in 708 urban children, those who had a BCG vaccine at birth were 0.05 (OR 0.05; 95% CI: 0.004 - 0.6) times less likely to have an SPT ≥ 7mm. The BCG unvaccinated cohort consisted of three individuals. There were no other significant associations between childhood vaccinations and food sensitization at SPT ≥ 1mm ,≥ 3mm and ≥ 7mm. There was no significant association between vaccinations and food allergy or other forms of atopy. In conclusion, there was very little evidence of an association between BCG vaccination in children and food allergic sensitisation or food allergy. However, in a small subgroup, there was evidence in an association between BCG and SPT ≥ 7 mm.
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Ahuja, Varun [Verfasser]. "Investigation of the sensitisation potential of various textile dyes using a biphasic mice local lymph node assay (LLNA) and an in vitro loose-fit coculture-based sensitisation assay (LCSA) / Varun Ahuja." Berlin : Freie Universität Berlin, 2010. http://d-nb.info/1024541894/34.
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Feng, Jian Qiang /. Sam, and S3069785@student rmit edu au. "The Effect of Acupuncture on Temporal Summation of Pain: A Randomised, Double-Blind, Sham-Controlled Study." RMIT University. Health Sciences, 2008. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080723.115945.
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There was few human study evaluated the analgesic effect of acupuncture on central nervous system (CNS). The electrical temporal summation (TS) pain model has been validated and provides the opportunity to study the central inhibition effect of acupuncture in healthy humans. The present study aimed to: 1. systematically review available randomised, controlled trials (RCTs) of acupuncture on experimentally induced pain in healthy humans; 2. conduct a RCT to assess the effect of manual acupuncture (MA) and electro-acupuncture (EA) on TS of pain and the spatial (i.e. the local and remote sites to acupuncture stimulation) and the temporal (i.e. immediately after and 24-hours after the intervention) characteristics of this effect. The systematic review was carried out in accordance with the requirements of a Cochrane Systematic Review. The methodological quality and credibility of the acupuncture intervention of the included RCTs were assessed. The Review Management software (RevMan version 4.2, The Cochrane Library) was used for data extraction and data analysis. 605 papers were identified from four databases (Pubmed, Cochrane Library, CINAHL and EMBASE). Only nine papers met the inclusion criteria. The methodological quality and credibility of the acupuncture invention were satisfactory. The pain models and interventions applied varied substantially from study to study. Consequently, meta-analyses were not conducted. Comparing acupuncture with non-invasive control, significant acupuncture analgesia was reported. These studies also demonstrated that invasive controls produced analgesia. For the RCT of acupuncture on TS, 27 healthy volunteers were recruited and randomly assigned to either EA, MA or sham-acupuncture (SA) group, with nine volunteers in each group. To test pain thresholds, transcutaneous electrical stimulation was delivered to two sites on the anterior aspects of both legs and one site on the dorsum of the non-dominant forearm. Pain thresholds to single electrical stimulation (SPT) and to TS stimulation (TST) were assessed before, 30-minutes after and 24-hours after the intervention. Acupuncture was given to Zusanli (ST36) and Fenglong (ST 40) on the dominant leg. The level of anxiety was assessed before and after acupuncture with Spielberg State and Anxiety Inventory. The three groups were comparable at baseline. The level of anxiety did not change significantly after acupuncture. EA significantly increased SPT and TST on the treatment leg 24-hour after the treatment when compared with SA (p less than 0.05), but did not increase those measured on the non-treatment leg or the forearm. The fact that such an effect increased within 24 hours after acupuncture might indicate the potential role of neurohumoral mechanisms in acupuncture analgesia. The analgesia effect of EA on TS tended to be localised at the needling site. This observation is different from the understanding of the wide-spread effect of acupuncture. The discrepancy could be due to the small sample size of the current study. In conclusion, this is the first study that demonstrates EA elicits a strong inhibition on the CNS in health humans. Such a central effect lasts more than 24 hours, and limits to the site where acupuncture is applied. These findings need to be confirmed in other TS models.
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Gideon, Abdullah Mohammed Abdul Fatah, and barrygideon@hotmail com. "Structural Characterisation, Residual Stress Determination and Degree of Sensitisation of Duplex Stainless Steel Welds." RMIT University. Civil, Environmental and Chemical Engineering, 2009. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20091110.101453.
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Welding of duplex stainless steel pipeline material for the oil and gas industry is now common practice. To date, research has been conducted primarily on the parent material and heat affected zones in terms of its susceptibility to various forms of corrosion. However, there has been little research conducted on the degree of sensitisation of the various successive weld layers, namely the root, fill and cap layers. The focus of this research study was to: (i) provide an in-depth microstructural analysis of the various weld passes, (ii) study the mechanical properties of the weld regions; (iii) determine degree of sensitisation of the various weld passes; and (iv) investigate the residual stress levels within the various regions/ phases of the welds. Four test conditions were prepared using manual Gas Tungsten Arc Welding with 'V' and 'U' bevel configuration. Structural analysis consisted of (i) optical microscopy, scanning electron microscopy and magnetic force microscopy; (ii) ferrite determination using Magna-Gauge, Fischer Ferrite-scope and Point Count method. Mechanical testing consisted of Vickers hardness measurements, Charpy impact studies and transverse tensile testing. The degree of sensitisation was determined by three test methods: a modified ASTM A262, ASTM A923 and a modified Double Loop Electrochemical Potentiodynamic Reactivation (DL-EPR) test. Residual stress levels were determined using two neutron diffraction techniques: a reactor source and a time of flight spallation source. Microstructure observed by optical microscopy and magnetic force microscopy shows the formation of both fine and coarse structures within the weld metal. There was no evidence of secondary austenite, being present in any of the weld metal conditions examined. In addition, no detrimental intermetallic phases or carbides were present. The DL-EPR test results revealed that the fill layer regions for all four conditions and the base material showed the highest values for Ir/Ia and Qr/Qa. All four test conditions passed the ASTM A262 and A923 qualitative type tests, even under restricted and modified conditions. Residual stress measurements by neutron diffraction conducted at Lucas Heights Hi-Flux Reactor revealed that the ferrite phase stress was tensile in the heat affected zones and weld, and appeared to be balanced by a local compressive austenite phase stresses in the normal and transverse directions. Residual stress measurements by neutron diffraction conducted at Los Alamos Nuclear Science Centre revealed that in the hoop direction, ferrite (211) and austenite (311) exhibit tensile strains in the weld. In the axial and radial direction, the strains for both phases were more compressive. Correlations between the degree of sensitization and microstructural changes / ferrite content were observed. Higher degrees of sensitization (Ir/Ia and Qr/Qa) were associated with reduced ferrite (increased austenite) content. Correlations between the stresses generated, the evolved microstructures and degree of sensitization were evident. Stresses within the cap region were generally shown to be of a tensile nature in the transverse and longitudinal direction. In summary, the study has shown that correlations exist between the weld microstructure, susceptibility to sensitisation and levels / distribution of internal stresses within the weld regions.
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Wylde, Vikki. "The role of pre-operative pain sensitisation in chronic pain after total knee replacement." Thesis, University of Bristol, 2010. http://hdl.handle.net/1983/3fc6eda2-7973-447b-b6d3-5610848780d5.
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Vance, Gillian Helen Sarah. "Early life exposure to a dietary allergen : characteristics, and consequences for allergic sensitisation and disease." Thesis, University of Lincoln, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269644.
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Bonnington, Jennifer Karen. "The role of nerve growth factor in rapid sensitisation of sensory neurones to painful stimuli." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620503.
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Aldossary, Sara Abdulrahman M. "The sensitisation of TRPV1 mediated responses by prostaglandin and bradykinin and the signalling pathways involved." Thesis, University of Leicester, 2015. http://hdl.handle.net/2381/33359.
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TRPV1 ion channels have crucial roles in inflammatory hyperalgesia as TRPV1-/- mice show reduced thermal hyperalgesia response in response to tissue inflammation. The activity of TRPV1 is upregulated in the case of inflammation due to the release of different inflammatory mediators which can modulate the function of TRPV1. Bradykinin is an algogenic substance which is released at the site of inflammation and it’s known to produce thermal hyperalgesia. However, the mechanism underlying the sensitising effect of bradykinin has not been full elucidated. The aim of present project is to investigate how bradykinin mediates the sensitisation of capsaicin evoked calcium responses in DRG neurons. With the use of immunocytochemical and morphometric techniques, the present study has demonstrated the expression and distribution of EP4, TRPV1, COX-1 and B2 proteins mainly in small diameter (<1,000μm2) cell bodies of rat DRG neurons which are typically nociceptors. Cultured rat DRG neurons were utilised to elucidate the direct and sensitising effects of bradykinin on sensory neurons. Following direct application of bradykinin, a group of cells evoked calcium responses which were mediated via the B2 receptor. In addition, bradykinin showed the ability to sensitise TRPV1 in the presence of thapsigargin, conditions under which bradykinin does not elicit a Ca2+ responses on its own. With the use of various pharmacological ligands that modulate prostaglandin biosynthesis, the formation of the second messenger, PGE2, acting through EP4 receptors was identified as a key signalling pathway mediating the effect of bradykinin on sensory neurons. The present study provides evidence for a novel signalling pathway through which bradykinin can regulate the TRPV1 ion channel function.
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Haus, Matthias. "Genetic and environmental influences on cord blood atopic markers and on atopic sensitisation in infancy." Doctoral thesis, University of Cape Town, 1988. http://hdl.handle.net/11427/27216.
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HISTORICAL PERSPECTIVE: It has recently been shown that intensive prophylactic dietary and environmental control measures during early infancy may reduce the incidence and/or postpone the onset of atopic disease. In order to institute this prophylactic regime, early identification of the infants genetically "at risk" for atopic disease is essential, since sensitisation begins at birth, or even during intra-uterine life. European and Scandinavian studies have shown that a raised concentration of cord blood serum immunoglobulin E (CBsIgE) is an excellent predictive marker for the subsequent development of atopic disease. Other potential predictive atopic markers such as cord blood eosinophils, platelets and anti-cow's milk serum IgG have also been suggested as having possible predictive relevance for newborns in terms of the development of subsequent atopic disease. PROBLEM DEFINITION: Most of the work in this field has been done on Caucasian neonates, in Westernised, First World countries. In South Africa, it has been shown that the Black adult ethnic group has serum immunoglobulin E concentrations (sIgE) which are significantly higher than that found in the South African White adult ethnic group. Furthermore, it has been suggested that the elevated sIgE in the adult Blacks may be raised independently of allergic disease. It is, therefore, important to ascertain whether this elevation of sIgE in Black South African adults is evident already at birth in the cord blood sera of Black South African newborns. If so, it is imperative to ascertain whether any such elevation is reflective of a high genetic load for atopy in these Black newborns, and furthermore whether these Black newborns are consequently "high-risk" for the development of subsequent atopic disease, as has been previously reported in the literature for White newborns. Arising from an awareness of these specific South African problems, the following hypothesis was developed. HYPOTHESIS: The hypothesis states that: "Black South African newborns without an atopic family history (aFH) have significantly higher CBslgE values than similar White and Mixed newborns. An aFH does not influence the CBsIgE values in the Black newborns, as it does in the White and Mixed newborns. The CBsIgE values in Black newborns are not, furthermore, predictive for the development of subsequent atopy in infancy, as they are in the other ethnic groups". A description of the three South African ethnic groups considered in this study is provided in Section IV, (Pg. 74). AIMS OF THE STUDY: The aims of the study were three-fold: 1. To test the hypothesis. 2. To assess the relevance of alternative cord blood markers (eosinophils, platelets and anti-cow's milk serum IgG) as predictive atopic markers in each of the three ethnic groups. 3. To provide epidemiological information with regard to genetic and environmental influences on CBslgE, cord blood total eosinophil counts (CBTEC's) cord blood platelet counts (CBPlC's) and cord blood anti-cow's milk serum IgG concentrations (CBacmlgG).
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Mirotti, Luciana Cristina. "The role of natural lipids in an in vivo model of sensitisation to Ber e 1." Thesis, University of Nottingham, 2010. http://eprints.nottingham.ac.uk/11530/.
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The prevalence of food allergy is increasing in westernized countries, affecting 5-8% of children and 1-3% of adults. Although innumerous proteins are encountered in normal diets, only few proteins are commonly implicated as food allergens. In this vein, the major focus in allergy studies falls into intrinsic features of allergens; however, it is known that extrinsic factors can play a role in allergic processes. The allergenicity of Ber e 1, the major allergen from Brazil nuts, is well established and it has been shown that natural lipids from Brazil nuts are essential for the development of an immune response towards Ber e 1. The present study aimed to characterize the humoral response induced by recombinant (r)Ber e 1 alone or in the presence of lipids, and to investigate the mechanism(s) by which natural lipids influence the development of an immune response. BALB/c mice were sensitised intraperitoneally with rBer e 1 alone or in the presence of different lipid fractions. It was found that rBer e 1 alone did not induce an immune response and only one specific fraction of Brazil nut lipids (SPC fraction C), composed of a mixture of lipid classes, was able to induce a Th2-type humoral response, with the presence of Ber-specific anaphylactic antibodies, high levels of Ber-specific IgG1, and low levels of Ber-specific IgG2a. CD1-restricted natural killer (NK)T cells recognize lipids and therefore to test the hypothesis that NKT cells may be involved in the response, the sensitisation protocol with rBer e 1 and SPC lipid fraction C was tested in mice lacking these cells (J18 KO mice). These animals presented significantly lower titers of Ber-specific anaphylactic antibodies, Ber-specific IgG1, and total IgE than sensitised wild type mice, indicating that one of the pathways by which lipids triggered an immune response involved NKT cells. In conclusion, the present work found that lipids from Brazil nuts were essential for the development of a Th2-type humoral response to rBer e 1 and that the immune response induced by lipids involved NKT cells.
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Bestall, Samuel. "Diabetic neuropathy : a mechanism of TRPV1 sensitisation and the treatment with Vascular Endothelial Growth Factor-A165b (VEGF-A165b)." Thesis, University of Nottingham, 2017. http://eprints.nottingham.ac.uk/47650/.
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Diabetic neuropathy affects up to 50% of diabetic patients and commonly presents as neuropathic pain. Streptozotocin (STZ) injected type 1 diabetic rats exhibit thermal hypersensitivity and this is caused by the sensitization of transient receptor potential vanilloid 1 (TRPV1) on DRG neurons. Thermal hypersensitivity is ameliorated in STZ diabetic rats with the systemic treatment of vascular endothelial growth factor-A165b (VEGF-A165b). This thesis investigated the role of the activation of the receptor for advanced glycated end products (RAGE) on the sensitization of TRPV1 on DRG neurons and determined the effects of VEGF-A165b on this mechanism. Capsaicin-evoked TRPV1 activity was measured in DRG neurons from adult STZ diabetic rats to determine a sensitization effect and these effects were modeled in vitro by exposing DRG neuronal cultures from naïve rats to hyperglycemic conditions. STZ diabetic rats had sensitized agonist-evoked TRPV1 activity, increased RAGE expression on the DRG neurons, and increased expression of high mobility group box-1 (HMGB1, a RAGE agonist) protein around nociceptor terminals. In vitro it was determined that hyperglycemic conditions sensitize capsaicin-evoked TRPV1 activity and this effect was mediated by the activation of RAGE, indicating that RAGE activation in diabetes is likely to cause TRPV1 sensitization. It was determined that HMGB1 binds to RAGE on DRG neurons and this binding results in the sensitization of TRPV1 activity and this sensitization event was a PKC mediated effect. PKC is able to phosphorylate serine residues on the intracellular domain of TRPV1. This phosphorylation causes increased agonist-evoked TRPV1 activity. Here it was demonstrated that there was increased TRPV1 phosphorylation at serine 800, a PKC dependent phosphorylation site, on DRG neurons in hyperglycemia and this event is likely to contribute towards the TRPV1 sensitization effect induced by HMGB1-RAGE binding. In vivo VEGF-A165b did not alter the expression of HMGB1 around nociceptor terminals but it did reduce the expression of RAGE on DRG neurons. In vitro VEGF-A165b blocked TRPV1 sensitization in DRG neurons exposed to hyperglycemic conditions, blocked the PKC mediated phosphorylation event, and blocked the HMGB1 mediated sensitization of TRPV1. VEGF-A165b may, therefore, be preventing this sensitization effect by blocking by the PKC activation that occurs downstream of RAGE activation. These results demonstrate a novel mechanism of neuronal TRPV1 sensitization in vitro involving the activation of RAGE on the DRG neurons. This mechanism may contribute to the sensitization of nociceptors in diabetes and consequently the development of neuropathic pain. VEGF-A165b blocks this mechanism indicating that 1) VEGF-A165b has direct actions on the DRG neurons in preventing them from hyperglycemia mediated damage and 2) VEGF-A165b may be exerting its analgesic effects in STZ diabetic rats through this mechanism.
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Meredith, Gavin Simon. "Friction stir processing for the reversal and mitigation of sensitisation and intergranular corrosion in aluminium alloy 5083-H321." Thesis, University of Birmingham, 2014. http://etheses.bham.ac.uk//id/eprint/4932/.
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AA5083-H321 is an aluminum alloy commonly used in ship hull superstructures as it has a corrosion resistance which affords an excellent degree of protection in chloride-rich marine environments. Corrosion performance can degrade in a process called sensitisation, due to the precipitation of a β-phase onto grain boundaries with exposure to elevated temperatures over decades of service. Friction Stir Processing (FSP) has been evaluated as a method for locally reversing the degraded microstructure and removing a susceptibility to Intergranular Corrosion (IGC) in immersed and atmospheric conditions. Both the mechanical stirring and heat input to the plate by an FSP tool have been shown to remove the β-phase from grain boundaries which had been precipitated by a sensitisation heat treatment. Sensitisation was shown to cause intergranular corrosion of the alloy; however this susceptibility was removed after microstructural modification by FSP. A re-sensitisation treatment of the previously sensitised and FSP’d region was seen to precipitate coarser and more discrete β-phase particles onto grain boundaries, which corroded at a faster rate than the once-sensitised material under electrochemical testing. This indicates that corrosion resistance degrades more quickly with subsequent sensitisation.
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Gray, Claudia Liesel. "The prevalence and patterns of IgE-mediated food allergy and sensitisation in South African children with atopic dermatitis." Doctoral thesis, University of Cape Town, 2014. http://hdl.handle.net/11427/12874.
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Includes bibliographical references.Background: The prevalence of food allergy in South Africa is unknown, but previously thought to be low, particularly in black South Africans. We hypothesised that food allergies would be low in Xhosa patients, even those at increased risk of food allergy such as children with atopic dermatitis (AD). This study aimed to determine the prevalence of, patterns and risk factors for, IgE-mediated food allergy in South African children with moderate to severe AD. It is the first food allergy prevalence study in South Africa to utilise controlled food challenges and component analysis, and is unique for its comparison of food allergy patterns between ethnic groups in the same geographical area. Methodology: This was a prospective, observational study in a paediatric university hospital in Cape Town. Children with moderate to severe AD, aged 6 months to 10 years, were randomly recruited from the dermatology clinic. They were assessed for sensitisation and allergy by questionnaire, skin prick tests (SPT), Immuno Solid Phase Allergen Chip (ISAC) test and incremental food challenges. Sensitised patients were also tested for specific IgE by ImmunoCAP test. Results: One hundred participants (59 black Africans and 41 of mixed race) were enrolled, median age 42 months. There were high overall rates of food sensitisation (66%) and food allergy (40%). Egg (25%) and peanut (24%) were the most common allergies. Black participants had comparable sensitisation (69% vs 61%) but lower allergy rates (34% vs 46%) than mixed race participants. This was especially evident for peanut allergy (15% vs 37%, p=0.01). Early onset AD (< 6 months), severe eczema, and young age < 2 years were significant risk factors for food allergy. The ISAC test was less sensitive than SPT and ImmunoCAP tests. Only 42% of cases of perceived food allergy were confirmed as true food allergy.
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Smyth, Lucy J. C. "Activation of cells of the mast cell/basophil lineage in response to potential allergens in the absence of IgE sensitisation." Thesis, University of Sheffield, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324450.
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Jeebhay, Mohamed Fareed. "Predictors of occupational sensitisation to grain dust allergens and changes in lung function among grain mill workers in Cape Town." Master's thesis, University of Cape Town, 1998. http://hdl.handle.net/11427/26261.
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Occupationally-related airway diseases, including asthma and chronic obstructive lung disease, have emerged as having substantial public health importance. The aim of this study was to identify the predictors of occupational sensitisation to grain dust allergens and changes in lung function among grain mill workers in Cape Town. There were two major objectives of the study. Firstly, to determine which of the following factors determine the distribution of serum ECP (eosinophilic cationic protein): age, gender, grain dust exposure, smoking status, atopy and sensitisation to workplace allergens. Secondly, to investigate the risk factors associated with the following outcomes: i) sensitisation to occupational allergens; ii) diagnosis of occupational asthma; iii) diagnosis of chronic obstructive airways disease; and iv) longitudinal changes in lung function. The risk factors studied included age, gender, smoking habits, occupational exposure, lung function status on baseline survey (1989), and allergic sensitisation assessed at follow up (1996). The methods employed involved a repeat measures cross-sectional design including a cohort followed up at different points over a seven year period. Survey instruments included a questionnaire, spirometry and allergy tests (phadiotop, RAST for wheat, rye, Lepidoglyphus destructor, Tyrophagus putrescentiae and Sitophilus granarius). The results indicated an association of grain dust with pulmonary function and allergic sensitisation to grain dust constituents. After adjusting for known confounders such as age, gender and smoking, significant associations were found between employment duration and both decrements in lung function and sensitisation to wheat grain. A decrement of 278 ml in FEY 1 and 328 ml in FYC was associated with occupational sensitisation to wheat (and rye). Increasing employment duration resulted in annual decrements of 18.3 ml in FEY1 and 23 ml in FYC for every year employed. The odds for developing occupational asthma was only mildly elevated (OR=l.35) with increasing employment duration. Age, however, was found to be protective (OR=0.85). Although we were unable to demonstrate a relationship between across-week changes in lung function, at inception, and rapid longitudinal lung function decline, our findings suggested that longitudinal change was related to the degree of airway obstruction at inception. Sensitisation to grain dust allergens was also found to be an independent predictor for FEY 1 and FYC. The prevalence of sensitisation was the highest for wheat (26.4%), followed by Tyrophagus putrescentiae (22.6%), rye (21.7%), Lepidoglyphus destructor (15.1 %) and Sitophilus granarius (15.1 %). Sensitisation to wheat was highly correlated with sensitisation to rye (r = 0.92) and so were Lepidoglyphus destructor and Tyrophagus putrescentiae (r = 0.85). Although a large proportion of the workforce ( 41.5 % ) were sensitised to occupational allergens, the prevalence of respiratory symptoms was between 15.6% and 23.9%. There were 16.7% of workers with health outcomes which fulfilled our criteria for occupational asthma. Atopic workers in our study had at least a nine-fold increased odds of becoming sensitised to grain dust allergens (OR: 8.9-74.7) and a two-fold increased odds of developing occupational asthma (OR= 1.9-84.9). Furthermore, the study found that smokers had a twofold increased odds of becoming atopic, thereby placing them at greater risk of developing respiratory health problems. The mean ECP in this population was 15.4 ug/1 (SD:2.5). Although 45.3% of the workers were atopic, it was not found to be predictor of elevated ECP levels. We were however able to demonstrate a significant association between ECP and sensitisation to grain allergens. Workers sensitised to wheat (positive RAST) had, on average, 1. 78 ug/1 higher ECP levels. The odds of having an elevated ECP (> 15 ug/1) increased by 2.9 for workers sensitised to wheat grain. In conclusion, the results of the study indicate that selection effects are in operation, demonstrating the health worker effect. The findings also suggest that across week reactions may be less sensitive than the across shift changes in predicting rapid longitudinal decline in lung function. While we were able to characterise the distribution of ECP according to exposure, we were however unable to define the temporal relationship between elevated between exposures, ECP and lung function outcomes due to limitations of the study design.
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Deshmukh, Abhijeet Popatrao. "The Role of Secreted Frizzled-related Protein-4 (sFRP4) in the Epigenetics, Metabolism and Chemo-sensitisation of Cancer Stem Cells." Thesis, Curtin University, 2017. http://hdl.handle.net/20.500.11937/65388.
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This study investigated molecular signals involved in sustaining CSCs and ways to target their activity using sFRP4 alone or in combination with chemotherapeutic drugs, possible involvement of methylation-mediated silencing of the sFRP gene family, and metabolic reprogramming by relocating metabolic flux to glycolysis or oxidative phosphorylation. sFRPs have an ability to sensitize tumour cells to chemotherapeutic drugs, thereby enhancing cell death. Altogether, sFRP4 treatment compromises the cell proliferation, and critically affects the cell survival mechanisms of CSCs.
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Jõgi, Rain. "Asthma : Respiratory Symptoms, Atopy and Bronchial Hyperresponsiveness in Young Adults in Estonia and Sweden." Doctoral thesis, Uppsala University, Department of Medical Sciences, 2001. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1417.
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Morbidity of asthma has increased over the world. The reasons for this increase have remained unclear. Studies in children have reported considerable East-West difference in the prevalence of atopy and respiratory allergies.
The aim of this thesis was to compare the prevalence and risk factors of respi-ratory symptoms, atopic sensitisation and bronchial hyperresponsiveness (BHR) in young adults in Estonia and Sweden.
Following the protocol of the European Community Respiratory Health Survey (ECRHS), two random population samples, 3000 from Tartu, Estonia, and 3600 from Uppsala, Sweden were investigated with postal questionnaires. Random sub samples and subjects with asthma-like complaints were subsequently interviewed, BHR was tested and serum samples analysed for total and specific IgE and eosinophil cationic protein (ECP). In a separate study two methacholine challenge methods, using either Spira Elektro2 or Mefar MB3 as dosimeters, were compared on 28 mild to moderate asthma patients.
Symptoms of asthma and hay fever were less common in Esto-nia than in Sweden, while respiratory symptoms in general were more common in Estonia. The prevalence of BHR was high and the prevalence of atopy and the levels of serum ECP were low in Tartu. The differences between the two centres in the prevalence of atopy and allergic rhinitis diminished with age, indicating a probable cohort effect. Current smoking was a dominant risk factor for BHR and for all respiratory symptoms, except attacks of asthma, both in Tartu and Uppsala. There was some difference between risk factors for BHR and atopy between Tartu and Uppsala, mostly of social and environmental origin. The low prevalence of hay fever and asthma in Tartu seemed to be partly explained by a lack of awareness of atopy and allergic diseases in the Estonian society. The estimated cumulative dose causing a 20% fall in FEV1 was smaller and the decline of FEV1 /log(dose) curve steeper, using the Spira, compared to the Mefar protocol.
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Bjerg, Bäcklund Anders. "Epidemiology of asthma in primary school children : the Obstructive Lung Disease in Northern Sweden (OLIN) studies thesis VIII." Doctoral thesis, Umeå universitet, Institutionen för folkhälsa och klinisk medicin, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1587.
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Background: Childhood asthma has increased worldwide, although recent studies report a prevalence plateau in some western countries. Aims: To investigate the prevalence of asthma and the associated risk factor patterns from ages 7-8 to 11-12 with special emphasis on the hereditary component, and further to study prevalence trends at age 7-8 from 1996 to 2006 and the possible determinants of these trends. Methods: The studies involved two cohorts from Kiruna, Luleå and Piteå: one previously identified cohort of 3430 children age 7-8 followed by yearly questionnaires until age 11-12 with 97% yearly participation. Skin-prick tests for allergic sensitisation were performed at ages 7-8 and 11-12 in subsets of 2148 and 2155 children respectively (88% of invited). In 2006 a new cohort of 7-8-year-olds was identified and examined identically. 2585 (96% of invited) and 1700 (90% of invited) participated in the questionnaire and skin-prick tests, respectively. The questionnaire included questions about symptoms of asthma, allergic rhinitis and eczema, and possible risk factors. Results: In the 1996 cohort, from age 7-8 to 11-12 the prevalence of physician-diagnosed asthma increased (5.7%-7.7%, P<0.01) while current wheeze decreased (11.7%-9.4%, P<0.01), and 34.7% reported ever wheee at ≥one occasion. Remission was 10% of which half relapsed during the study. Remission was significantly lower among sensitised children. The strongest risk factors for current asthma at ages 7-8 and 11-12 were allergic sensitisation (OR 5) and family history of asthma (OR 3). Several other significant risk factors, e.g. respiratory infections, damp house and low birth weight, had lost importance at age 11-12. At age 7-8, parental asthma was a stronger risk factor (OR 3-4) than parental rhinitis or eczema (OR 1.5-2). Sibling asthma had no independent effect. Biparental asthma had a multiplicative effect (OR 10). Maternal and paternal asthma was equally important, regardless of the child’s sex and sensitisation status. From 1996 to 2006 the prevalence of current wheeze and asthma at age 7-8 did not increase (P=0.13, P=0.18), while lifetime prevalence of ever wheeze and physician-diagnosed asthma increased (P<0.01, P=0.01). Symptoms of rhinitis and eczema were unchanged, despite 45% increase (P<0.01) in allergic sensitisation. For current asthma the adjusted population attributable fractions of sensitisation and parental asthma increased (35%-41%, 27%-45%). This was however balanced by decreased exposure to infections, maternal smoking and home dampness, resulting in stable asthma prevalence. Stratification by sex revealed that current wheeze increased in boys (P<0.01) but tended to decrease in girls (P=0.37), seemingly due to symptom persistence in males. Several asthma indices followed this pattern. The boy-to-girl ratio in exposure to all studied risk factors increased, which may explain the sex-specific prevalence trends in wheeze. Conclusions: The prevalence of current asthma and wheeze did not increase statistically significantly. However, the risk factor pattern has changed considerably since 1996, which will presumably affect the clinical features of childhood wheeze in this region. Sex-specific trends in wheeze can be explained by changes in exposure, and trends in risk factors should be explored parallel to prevalence trends.
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Mezue, Melvin Nnanyelu. "Defining the neural correlates of pain and analgesia in health and disease." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:e8dfd540-b991-49fa-8b02-0ae21e2086e0.
Full textAbstract:
Chronic neuropathic pain affects up to 8% of the United Kingdom population and is a difficult condition to manage. It is established and maintained through many mechanisms, including central sensitisation (CS) in the spinal cord and brainstem. Neuropathic pain manifests as spontaneous pain, sensory loss and evoked hypersensitivity. The development of novel treatments for neuropathic pain is challenging, in part due to inadequate experimental models of clinically relevant pain. The use of functional magnetic resonance imaging (fMRI) techniques for imaging acute and increasingly tonic states enables the assessment of the neural correlates of evoked hypersensitivity and persistent pain, with the goal of developing appropriate biomarkers to test new therapies. This thesis develops novel techniques for the assessment of ongoing pain states and their modulation by therapies. We first identified a suitable human experimental model of CS using topical capsaicin, and an fMRI pipeline for the investigation of supraspinal involvement in pain hypersensitivity. In a placebo-controlled study, we then demonstrated the improved sensitivity of fMRI above subjective reports in detecting the efficacy of a known analgesic as compared to an ineffective active compound in a small cohort. To translate this to the more clinically relevant symptom of spontaneous pain, we developed and validated the use of a multi-inversion time pseudo-continuous arterial spin labelling (ASL) imaging and analysis pipeline for the neural assessment of tonic states and the absolute quantification of cerebral blood flow (CBF). Current evidence from structural and functional studies suggests a direct role for the posterior insula cortex in the encoding of nociception and pain. Using the ASL pipeline, we found that only a CBF change in the posterior insula region was correlated with the changing perception of persistent capsaicin-induced pain, and in a separate experiment showed that suppression of CBF in this region by gabapentin was related to the drug's suppression of subjective pain perception. We also demonstrated in a cohort of phantom limb patients that pain relief resulting from transcranial direct current stimulation of the deprived sensorimotor cortex is neurally represented by a decrease in posterior insula CBF. In a separate study, we showed that baseline CBF in the periaqueductal grey can predict individuals who are most vulnerable to pain and hypersensitivity following the induction of capsaicin-related CS. Taken together, these findings suggest that fMRI can be used as a tool to assess the efficacy of established and novel analgesics, with the midbrain reticular formation and posterior insula cortex being prime candidates as biomarkers of CS mechanisms and persistent pain respectively. Relatedly, ASL-fMRI may also be an effective technique for evaluating individuals' susceptibility to pain following inflammation or injury.
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Ribeiro, Ana Catarina de Guimarães. "Comparative study of the prevalence, clinical features, sensitisation profiles and risk factors for Allergic Rhinitis between elderly and young in Cova da Beira." Master's thesis, Universidade da Beira Interior, 2010. http://hdl.handle.net/10400.6/854.
Full textAbstract:
Background: Allergic Rhinitis (AR) is the most common allergic disease worldwide. Very few
studies have analysed whether the prevalence of AR decreases with age. Therefore, the aim of
the present study was to compare the prevalence, pattern of aeroallergen sensitisation and
risk factors for AR between elderly and young adults.
Method: This was a cross-sectional study using a simple random sample. The population
consisted of 2 groups of individuals from Beira Interior: one of young adults (aged between 18
and 35 years) and another of elderly individuals (aged 65 years or older). A standardised
allergy and rhinitis questionnaire as well as skin prick tests (SPT) were carried out in all
volunteers, except for those who answered the questionnaire by telephone. All patients signed
a written informed consent and the study was approved by the Regional Health Authority
Ethics Committee. Chi-square test, Mann Whitney U test and logistic regression test were used
for statistical analysis. A p value less than 0.05 was considered statistically significant.
Results: Our study sample included 1460 volunteers. To date, 473 volunteers have been
analysed (312 elderly (median age = 72 years; 168 females) and 161 young adults (median age
= 29 years; 85 females). Both groups were paired regarding gender. The prevalence of AR was
significantly lower in elderly (26.7%) than in young adult volunteers (40.6%) (p=0.0194; Chisquare test). For both groups, association between overall positivity of SPT and self-reported
symptoms of AR was highly significant (p<0.0001 for elderly; p=0.0069 for young adults).
Significant differences were observed in the sensitisation patterns between the two groups
with the elderly being mostly sensitised to Dermatophagoides pteronyssinus (11.0%),
Parietaria judaica (10.5%) and Olive tree (7.3%) and young adults mostly to Dermatophagoides
pteronyssinus (25.7%), Dermatophagoides farinae (15.8%) and cereal pollen (16.8%). A
significant association was found between AR and urban residence in the elderly group
(p=0.047; Chi-square) Conclusions: Our data suggest that the prevalence of AR decreases with age and also that
there may be differences in the profile of sensitisation to aeroallergens between young and
elderly individuals.Contextualização: A rinite Alérgica (RA) é a doença alérgica mais comum em todo o mundo. Muito poucos estudos têm avaliado se a prevalência da RA diminui com a idade. Assim sendo, o objectivo deste estudo foi comparar a prevalência, o padrão de sensibilização a aeroalergénios e factores de risco da RA entre uma população de idosos e outra de adultos jovens. Métodos: Estudo transversal usando uma amostra aleatória simples. A população do estudo consistiu em 2 grupos de indivíduos da Beira Interior: um de adultos jovens (com idades entre os 18 e 35 anos) e outro de idosos (com 65 anos ou mais). Um questionário estandardizado de alergia (incluindo RA) e testes cutâneos de alergia (TCA) foram aplicados a todos os voluntários, à excepção dos que participaram por telefone. Todos os voluntários assinaram o consentimento informado e o estudo foi aprovado pelo Comité de Ética da Autoridade Regional de Saúde. Para a análise estatística recorreu-se aos seguintes testes: Qui-quadrado, Mann Whitney U e regressão logística. Um valor de p < 0,05 foi considerado estatisticamente significativo. Resultados: A população do nosso estudo incluiu 1460 voluntários. Até agora, foram analisados 473 voluntários (312 idosos (idade média = 72 anos; 168 mulheres) e 161 adultos jovens (idade média = 29 anos; 85 mulheres). Ambos os grupos estavam emparelhados quanto à distribuição por sexos. A prevalência de RA foi significativamente menor nos idosos (26,7%) em relação aos adultos jovens (40,6%) (p=0,0194; teste do Qui-quadrado). Para ambos os grupos, a associação entre positividade geral dos TCAs e os sintomas de RA foi estatisticamente significativa (p<0,0001 para idosos; p=0,0069 para adultos jovens). Diferenças significativas foram observadas no perfil de sensibilização entre os 2 grupos, estando os idosos mais sensibilizados a Dermatophagoides pteronyssinus (11,0%), Parietaria judaica (10,5%) e oliveira (7,3%) e os adultos jovens principalmente a Dermatophagoides pteronyssinus (25,7%) Dermatophagoides farinae (15,8%) e pólenes de cereais (16,8%). Uma associação estatisticamente significativa foi encontrada entre RA e residência urbana no grupo dos idosos (p=0,047; teste do Qui-quadrado). Conclusão: Os nossos dados sugerem que a prevalência da RA diminui com a idade e também que parecem existir diferenças entre os perfis de sensibilização de idosos e adultos jovens.
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Moreira, Sónia Manuela Rodrigues. "Comparative study of the prevalence, clinical features, sensitisation profiles and risk factors for bronchial asthma between elderly and young adults in Cova da Beira." Master's thesis, Universidade da Beira Interior, 2010. http://hdl.handle.net/10400.6/803.
Full textAbstract:
Background: Bronchial asthma is one of the most common allergic diseases
worldwide. Very few studies have analysed whether the prevalence of asthma decreases
with age. Therefore, the aim of the present study was to compare the prevalence, pattern
of aeroallergen sensitization and clinical features of bronchial asthma between elderly
and young adults. In addition, we also wanted to analyse possible risk factors for the
development of asthma.
Methods: This study followed a cross-sectional design. A standardised allergy and
asthma questionnaire and skin prick tests (SPT) were carried out in all volunteers. The
study population included two groups of individuals: elderly (aged 65 years and older)
and young adults (aged between 18-35 years) from Beira Interior. The sample was
selected by simple randomization, after calculating a confidence interval of 95% and an
estimated error below 5%. Statistical analysis was carried out using Chi-square tests,
Mann-Whitney U test, univariate regression analysis, binary logistic regression analysis
and Odds Ratio. A p value less than 0.05 was considered statistically significant. All
patients signed a written informed consent and the study was approved by the Regional
Health Authority Ethics Committee.
Results: A total sample of 1460 volunteers was included. Thus far, we have analyzed a
total of 27.6% of elderly volunteers in the Health Care Centre (median age = 73 years;
41.5% males) and 14.1% of the young group (median age = 28 years; 44.2% males). A
short questionnaire was applied by telephone to 11% of the total sample. We found
significant differences in the prevalence of bronchial asthma between elderly and young
adults (19.7% vs 29.4%, respectively; p=0.02; Chi-square test). In addition, the
prevalence of atopic bronchial asthma was also significantly lower in elderly than in young adult patients (32.1% vs 57.1%, respectively; p=0.02, Chi-square test). Both
groups were mostly sensitised to Dermatophagoides pteronyssinus and
Dermatophagoides farinae. Significant differences were found in terms of sensitisation
to grass pollens amongst elderly and young patients (11.8% vs 70.8%, respectively;
p=0.0006, Chi-square test). The majority of the asthmatic patients were sensitised to
more than one allergen. Finally, differences were observed in terms of risk factors for
bronchial asthma between both groups, with gender as a significant risk factor in elderly
patients and concomitant rhinitis in young patients. The presence of rhinitis augmented
the risk of having asthma 3.2 times, in the group of young adults.Introdução: A asma brônquica é uma das doenças mais comuns a nível mundial. Poucos estudos analisaram se a sua prevalência diminui com a idade. Assim sendo, o principal objectivo deste estudo foi comparar a prevalência, perfil de sensibilização, características clínicas e possíveis factores de risco para o desenvolvimento da asma em idosos e adultos jovens. Métodos: Estudo transversal que implicou a realização de questionário padrão sobre alergia e asma, bem como realização de testes cutâneos de alergia (TCA) a todos os voluntários. A população do estudo abrangeu dois grupos de indivíduos: idosos (com idade igual ou superior a 65 anos de idade) e adultos jovens (com idades compreendidas entre 18 e 35 anos) da Beira Interior. A amostra foi calculada através de randomização simples. A análise estatística consistiu na aplicação dos testes de Qui-quadrado, MannWhitney U, análise de regressão univariável, análise de regressão logística binária e Odds Ratio. Um valor de p abaixo de 0,05 foi considerado estatisticamente significativo. Todos os voluntários que participaram nesta investigação assinaram consentimento informado e o estudo foi aprovado pelo Comité de Ética da Autoridade Regional de Saúde. Resultados: A população do estudo consistiu em 1460 voluntários. Contudo, até ao momento, apenas analisámos um total de 27,6% de idosos no centro de saúde (idade média = 73 anos; 41,5% sexo masculino) e 14,1% do grupo de jovens (idade média 28; 44,2% sexo masculino). Foi aplicado um questionário simples por telefone a 11% do total da amostra. Encontrámos diferenças significativas relativas à prevalência da asma entre idosos e adultos jovens (19,7% vs 29,4%, respectivamente; p = 0,02; teste do Quiquadrado). Para além disto, a prevalência de asma brônquica atópica foi significativamente menor em idosos que em adultos jovens (32,1% vs 57,1%, respectivamente p=0,02). Ambos os grupos de doentes asmáticos demonstraram elevada sensibilização para Dermatophagoides pteronyssinus e Dermatophagoides farinae. Encontrámos diferenças significativas na sensibilização dos idosos e jovens para pólenes de ervas (11,8% vs 70,8%, respectivamente; p=0,0006, teste do Qui-quadrado). A maioria dos doentes asmáticos estava sensibilizada a mais de um alergéneo. Finalmente, foram constatadas diferenças relativamente aos factores de risco para desenvolvimento de asma nos diferentes grupos, sendo o sexo significativo no caso dos idosos e a concomitância de rinite nos jovens. A presença de rinite aumentou em 3,2 vezes a probabilidade de desenvolver asma nos adultos jovens.
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Vetma, Vesna [Verfasser], and Markus [Akademischer Betreuer] Morrison. "Assessment of TRAIL sensitisation by IAP antagonist TL32711 in malignant melanoma and development of a framework for response prediction / Vesna Vetma ; Betreuer: Markus Morrison." Stuttgart : Universitätsbibliothek der Universität Stuttgart, 2020. http://d-nb.info/1212034449/34.
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