Dissertations / Theses on the topic 'Seminal vesicles'
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陳德華 and Tak-wah Chan. "Epithelial-mesenchymal interactions in development and cytodifferentiation of seminal vesicle." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1994. http://hub.hku.hk/bib/B31211239.
Full textChan, Tak-wah. "Epithelial-mesenchymal interactions in development and cytodifferentiation of seminal vesicle /." Hong Kong : University of Hong Kong, 1994. http://sunzi.lib.hku.hk/hkuto/record.jsp?B13762692.
Full textWilliams, R. L. "Organisation and control of androgen-responsive genes of rat seminal vesicles." Thesis, University of Leeds, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355711.
Full textSchultheiss, Willem Andreas. "Some aspects of the aetiology of vesiculitis in a Sussex herd." Pretoria : [s.n.], 1998. http://explore.up.ac.za/record=b1411086.
Full textTam, Chuen-chu. "Hormonal effects of the lateral prostate and seminal vesicle of the guinea pig : an ultrastructural, morphometric and cytochemical study /." Hong Kong : University of Hong Kong, 1989. http://sunzi.lib.hku.hk/hkuto/record.jsp?B12418833.
Full textSilva, Yamê Fabres Robaina Sancler da [UNESP]. "Efeito do tratamento local de vesiculite seminal sobre a qualidade e longevidade so sêmen equino." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/110631.
Full textA vesiculite seminal possui grande relevância na clínica reprodutiva devido à dificuldade de tratamento, elevados índices de recidiva, risco de contaminação de fêmeas com agentes patogênicos, inutilização de animais e baixos índices de fertilidade. O tratamento local tem sido apontado por diversos autores como a melhor alternativa terapêutica, porém nenhum estudo avaliou seus efeitos na qualidade e longevidade seminal. Nesse sentido, os objetivos do presente estudo incluíram: investigar quais são as principais bactérias envolvidas na enfermidade e os principais fatores de risco para os garanhões; avaliar e comparar o efeito do tratamento local de vesiculite seminal quanto aos índices de cinética e morfologia espermática, integridade de membrana plasmática, porcentagem de leucócitos e contagem de unidades formadoras de colônias (UFC) de amostras seminais frescas, refrigeradas e congeladas e o teor de óxido nítrico no plasma seminal, antes (M0), após uma semana (M1) e um mês (M2) da terapia. Os resultados obtidos permitiram inferir que a vesiculite seminal foi mais prevalente em animais adultos e idosos, a evolução da enfermidade ocorreu de forma crônica em todos os animais selecionados, a monta natural constituiu um fator predisponente para o surgimento da doença, a coloração amarelada do sêmen e a dificuldade na ejaculação são sinais indicativos de alteração da glândula e a bactéria mais prevalente envolvida na etiopatogenia da infecção foi a Pseudomonas aeruginosa. De maneira geral, o tratamento local levou a uma melhora da qualidade seminal após uma semana (M1), no sêmen a fresco, refrigerado e congelado, quanto aos índices de cinética espermática e integridade de membrana plasmática (p<0,05). No sêmen a fresco promoveu redução do volume seminal, incremento da concentração espermática, redução da porcentagem de leucócitos e de UFC/mL no M1 em relação aos demais momentos ...
Seminal vesiculitis has great relevance in reproductive clinic due to the difficulty of treatment, high rates of recurrence, risk of female contamination with pathogenic agents, retirement of animals and low fertility rates. Local treatment has been reported as the best therapeutic alternative, although no other studies have evaluated its effects on seminal quality and longevity. The aims of this study included: to investigate what are the main bacteria involved in the disease and the risk factors for stallions; to evaluate and to compare the effect of local treatment of seminal vesiculitis on the sperm kinetic parameters, morphology and viability (plasma membrane integrity), percentage of leukocytes and counting colony forming units (CFU) of fresh, cooled and frozen semen samples and the content of nitric oxide in the seminal plasma, before (M0) and after one week (M1) and one month (M2) therapy. The results allow us to infer that the seminal vesiculitis was more prevalent in adults and aged animals, the evolution of the disease appeared chronically in all selected animals, natural breeding was a risk factor for onset of the disorder, the bege coulor of semen and ejaculatory dysfunction were signs indicative of abnormalities in the gland and the most common bacteria involved in the pathogenesis of the infection was Pseudomonas aeruginosa. In general, the local treatment produced an improvement in semen quality after a week (M1) in fresh, cooled and frozen semen on the kinetic parameters and sperm viability (p<0.05). In fresh semen promoted reduction in the volume of ejaculate, sperm concentration increased, reducing the percentage of leukocytes and CFU/mL in M1 compared to the other moments (p<0.05). In frozen semen promoted reduction of lipid peroxidation and of ROS content in M1 compared to other moments (p<0.05). The cooled semen for 24 hours at 5°C and 15°C demonstrated similar efficiency in the pre and post-treatment ...
Silva, Yamê Fabres Robaina Sancler da. "Efeito do tratamento local de vesiculite seminal sobre a qualidade e longevidade so sêmen equino /." Botucatu, 2014. http://hdl.handle.net/11449/110631.
Full textBanca: João Carlos Pinheiro Ferreira
Banca: André Maciel Crespilho
Resumo: A vesiculite seminal possui grande relevância na clínica reprodutiva devido à dificuldade de tratamento, elevados índices de recidiva, risco de contaminação de fêmeas com agentes patogênicos, inutilização de animais e baixos índices de fertilidade. O tratamento local tem sido apontado por diversos autores como a melhor alternativa terapêutica, porém nenhum estudo avaliou seus efeitos na qualidade e longevidade seminal. Nesse sentido, os objetivos do presente estudo incluíram: investigar quais são as principais bactérias envolvidas na enfermidade e os principais fatores de risco para os garanhões; avaliar e comparar o efeito do tratamento local de vesiculite seminal quanto aos índices de cinética e morfologia espermática, integridade de membrana plasmática, porcentagem de leucócitos e contagem de unidades formadoras de colônias (UFC) de amostras seminais frescas, refrigeradas e congeladas e o teor de óxido nítrico no plasma seminal, antes (M0), após uma semana (M1) e um mês (M2) da terapia. Os resultados obtidos permitiram inferir que a vesiculite seminal foi mais prevalente em animais adultos e idosos, a evolução da enfermidade ocorreu de forma crônica em todos os animais selecionados, a monta natural constituiu um fator predisponente para o surgimento da doença, a coloração amarelada do sêmen e a dificuldade na ejaculação são sinais indicativos de alteração da glândula e a bactéria mais prevalente envolvida na etiopatogenia da infecção foi a Pseudomonas aeruginosa. De maneira geral, o tratamento local levou a uma melhora da qualidade seminal após uma semana (M1), no sêmen a fresco, refrigerado e congelado, quanto aos índices de cinética espermática e integridade de membrana plasmática (p<0,05). No sêmen a fresco promoveu redução do volume seminal, incremento da concentração espermática, redução da porcentagem de leucócitos e de UFC/mL no M1 em relação aos demais momentos ...
Abstract: Seminal vesiculitis has great relevance in reproductive clinic due to the difficulty of treatment, high rates of recurrence, risk of female contamination with pathogenic agents, retirement of animals and low fertility rates. Local treatment has been reported as the best therapeutic alternative, although no other studies have evaluated its effects on seminal quality and longevity. The aims of this study included: to investigate what are the main bacteria involved in the disease and the risk factors for stallions; to evaluate and to compare the effect of local treatment of seminal vesiculitis on the sperm kinetic parameters, morphology and viability (plasma membrane integrity), percentage of leukocytes and counting colony forming units (CFU) of fresh, cooled and frozen semen samples and the content of nitric oxide in the seminal plasma, before (M0) and after one week (M1) and one month (M2) therapy. The results allow us to infer that the seminal vesiculitis was more prevalent in adults and aged animals, the evolution of the disease appeared chronically in all selected animals, natural breeding was a risk factor for onset of the disorder, the bege coulor of semen and ejaculatory dysfunction were signs indicative of abnormalities in the gland and the most common bacteria involved in the pathogenesis of the infection was Pseudomonas aeruginosa. In general, the local treatment produced an improvement in semen quality after a week (M1) in fresh, cooled and frozen semen on the kinetic parameters and sperm viability (p<0.05). In fresh semen promoted reduction in the volume of ejaculate, sperm concentration increased, reducing the percentage of leukocytes and CFU/mL in M1 compared to the other moments (p<0.05). In frozen semen promoted reduction of lipid peroxidation and of ROS content in M1 compared to other moments (p<0.05). The cooled semen for 24 hours at 5°C and 15°C demonstrated similar efficiency in the pre and post-treatment ...
Mestre
Davey, Tamara. "Functional characterisation of a novel osteoclast-derived factor." University of Western Australia. School of Surgery and Pathology, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0219.
Full textSahlén, Göran. "Formation,Storage and Secretion of Prostasomes in Benign and Malignant Cells and Their Immunogenicity in Prostate Cancer Patients." Doctoral thesis, Uppsala University, Department of Surgical Sciences, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7511.
Full textProstasomes are submicron-sized, membrane-bound organelles produced by the epithelial cells of the prostate and normally found in the secretion in the gland ducts. Their physiological role is in the promotion of sperm-function in human reproduction. This thesis contains four papers dealing with the production of prostasomes and some possible applications in clinical urology of the prostasome.
Paper I and II provided an ultrastructural description of the synthesis, storage and secretion of prostasomes in benign as well as in malignant tissue. Most notable were the extracellular appearances of prostasomes in metastatic lesions whereby the prostasomes become exposed to the immune system of the patient. This supported findings in earlier studies in which patients with advanced prostate cancer had elevated levels of anti-prostasome antibodies. The results of paper III reinforced the view of the prostate-unique origin of the prostasome. In particular, there were no indications in SDS-PAGE patterns or flow-cytometric studies of material from seminal vesicle secretion that it contained components that could be associated with a production of prostasomes.
Some possible clinical functions of the prostasomes were investigated in paper IV. Exposure of prostasomes to the immune system through mechanical and thermal trauma to the prostate did not induce an evident formation of anti-prostasome autoantibodies. Furthermore, the serum levels of anti-prostasome antibodies registered by assays with preparations of prostasomes from seminal plasma as antigen did not correlate with existing prostate cancer. Seminal prostasomes seemed not to function as substitute markers for prostate cancer in the test kit used. A possible explanation could be underestimated differences in antigen properties between seminal or prostate gland-derived prostasomes and prostasomes from tumor tissue.
陳良 and Leung Franky Chan. "A morphological, histochemical and experimental study of the prostate gland and seminal vesicles of the guinea pig, with special referenceto the stroma." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1989. http://hub.hku.hk/bib/B30425773.
Full textKim, Julie M. "Androgen-induced norepinephrine release in male accessory sex organ smooth muscle growth and differentiation." Morgantown, WV : [West Virginia University Libraries], 1999. http://etd.wvu.edu/templates/showETD.cfm?recnum=417.
Full textTitle from document title page. Document formatted into pages; contains vi, 125 p. : ill. Vita. Includes abstract. Includes bibliographical references (p. 107-122).
Chan, Leung Franky. "A morphological, histochemical and experimental study of the prostate gland and seminal vesicles of the guinea pig, with special reference to the stroma /." [Hong Kong : University of Hong Kong], 1989. http://sunzi.lib.hku.hk/hkuto/record.jsp?B12439794.
Full text譚銓株 and Chuen-chu Tam. "Hormonal effects of the lateral prostate and seminal vesicle of the guinea pig: an ultrastructural, morphometricand cytochemical study." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1989. http://hub.hku.hk/bib/B3123169X.
Full textBarrachina, Villalonga Ferran. "Proteòmica del plasma seminal i de les seves vesícules extracel·lulars: nova font de biomarcadors útils en l’estudi de la funció espermàtica i la infertilitat masculina." Doctoral thesis, Universitat de Barcelona, 2020. http://hdl.handle.net/10803/672925.
Full textInfertility is a common and rising problem worldwide. However, the lack of understanding of male reproductive biology and the molecular mechanisms altered in infertile patients results in a limited and insufficient availability of male infertility diagnostic and prognostic tools, as well as fertility treatments. Although the sperm is the key piece in the transmission of paternal information to the embryo, there is evidence that the fluids secreted by the epididymis and male accessory sex glands, including the seminal EVs, play a pivotal role in male reproduction more than simply being a medium to carry the spermatozoa. The hypothesis of this thesis is that the use of high-throughput proteomic techniques for the study of the seminal plasma of infertile patients could allow the identification of novel and non-invasive clinical biomarkers, which could result in an improvement of the current male infertility stratification and an optimization of their diagnostic and treatment, moving towards a personalized evaluation of male infertility. Also, the study of the intercellular communication between seminal EVs and sperm, and the application of new methodologies to obtain specific populations of the different types of EVs, could contribute to revealing the real biological roles of seminal EVs from the epididymis and the male accessory sex glands in sperm function. These findings could provide the basis for future studies aimed at identifying new diagnostic and prognostic biomarkers, as well as developing new therapies to improve male fertility. Therefore, the objectives of this doctoral thesis are: 1. To analyze the seminal plasma proteomic profile of patients with different types of infertility in order to identify new potential biomarkers of fertility/infertility useful for clinical care. 1.1. To define the seminal plasma proteome signatures of infertile patients categorized according to their seminal parameters (normozoospermia, asthenozoospermia, oligozoospermia, and azoospermia). 1.2. To characterize the seminal plasma proteome of infertile patients with hypogonadotropic hypogonadism, before and after testosterone replacement therapy. 2. To explore the potential intercellular communication between seminal EVs and sperm, and to determine the impact of this on sperm function. 2.1. To demonstrate the participation of epididymosomes (EVs secreted by the epididymis) in protein transfer between epididymis and sperm. 2.2. To determine the impact of CD63+ seminal EVs on sperm function by isolating the EVs with immunoaffinity techniques, and to characterize their protein content by high-throughput proteomics.
Кравчук, О. М. "Вплив гіпертермії різного ступеня на органометричні параметри сім'яних пухирців статевонезрілих щурів." Thesis, Сумський державний університет, 2015. http://essuir.sumdu.edu.ua/handle/123456789/41704.
Full textNUGYEN, QUOC HIEN GEORGES. "Imagerie des vesicules seminales." Lille 2, 1990. http://www.theses.fr/1990LIL2M242.
Full textBUTTIN, FRANCOIS-XAVIER. "Vesiculectomie seminale : comment, pourquoi ?" Lyon 1, 1994. http://www.theses.fr/1994LYO1M055.
Full textFawell, S. E. "Androgenic regulation of secretory protein synthesis in rat seminal vesicle." Thesis, University of Leeds, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355701.
Full textKessler, Damien. "L'adenocarcinome des vesicules seminales : a propos de deux cas : revue de la litterature." Université Louis Pasteur (Strasbourg) (1971-2008), 1991. http://www.theses.fr/1991STR1M199.
Full text呂小楓 and Xiaofeng Lu. "Changes in cytodifferentiation of the dunning prostatic adenocarcinomainduced by neonatal rat seminal vesicle mesenchyme." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31215610.
Full textLu, Xiaofeng. "Changes in cytodifferentiation of the dunning prostatic adenocarcinoma induced by neonatal rat seminal vesicle mesenchyme /." Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19852216.
Full textSeidensticker, Mathias [Verfasser]. "Drug Effects on the Excretory Ductal System of the Prostate, Seminal Vesicle and Epididymis / Mathias Seidensticker." Gießen : Universitätsbibliothek, 2020. http://d-nb.info/1209159805/34.
Full textCOLUNA, João Marcelo Martins. "Efeitos do uso de probiótico sobre a toxicidade do dicromato de potássio no sistema reprodutor masculino e adrenais de ratos Wistar." Universidade do Oeste Paulista, 2014. http://bdtd.unoeste.br:8080/jspui/handle/jspui/1050.
Full textMade available in DSpace on 2017-08-31T20:12:04Z (GMT). No. of bitstreams: 1 João Marcelo Martins Coluna.pdf: 316916 bytes, checksum: e3ff1f0c4795f8a26f2d99c916a84f41 (MD5) Previous issue date: 2014-03-24
The aim of this study was to evaluate the effect of probiotics on the toxicity of increasing doses of potassium dichromate on the reproductive tract and adrenal glands of rats . Material and Methods: During a trial period of 90 days , we used 80 Wistar rats were divided randomly into 8 groups of 10 animals : GC (control ) , G1 (12 mg.kg - 1 K2Cr2O7 ) ; G24 ( 24 mg.kg - 1 K2Cr2O7 ) ; G36 ( 36 mg.kg - 1 K2Cr2O7 ) ; GP Control ( 0.2% probiotic ); G12P ( 12 mg.kg - 1 K2Cr2O7 + Probiotic 0.2 % ) ; G24P ( 24 mg.kg - 1 K2Cr2O7 + Probiotic 0.2 % ) ; G36P ( 36 mg.kg - 1 K2Cr2O7 Probiotic + 0.2% ) . Subsequently , weighing and histological evaluation of the reproductive organs and adrenal glands was performed , and plasma testosterone dosage . Results: Testes were heavier in group 7 There were statistical differences between the weights of the seminal vesicles only in groups 2:07 Histologically observed development of atypical hyperplasia and squamous metaplasia in the testes and seminal vesicles and degeneration of the germinal epithelium and tubular contraction testes despite the use of probiotics. Conclusion : The use of probiotics has not protected the epithelium of the reproductive system in this study .
O objetivo deste estudo foi avaliar o efeito do probiótico sobre a toxicidade de doses crescentes de dicromato de potássio sobre o trato reprodutivo e glândulas adrenais de ratos Wistar. Material e Métodos: Durante um período experimental de 90 dia, utilizou-se 80 ratos Wistar distribuídos aleatoriamente em 8 grupos de 10 animais: GC (controle), G12 (12 mg.kg-1 K2Cr2O7); G24 (24 mg.kg-1 K2Cr2O7); G36 (36 mg.kg-1 K2Cr2O7); GP (Controle, 0,2% probiótico); G12P (12 mg.kg-1 K2Cr2O7 + 0,2% Probiótico); G24P (24 mg.kg-1 K2Cr2O7 + 0,2% Probiótico); G36P (36 mg.kg-1 K2Cr2O7 + 0,2% Probiótico). Posteriormente foi realizada, pesagem e avaliação histológica dos órgãos reprodutivos e das glândulas adrenais, bem como dosagem de testosterona plasmática. Resultados: Os testículos pesaram mais no grupo 7. Houve diferença estatística entre os pesos das vesículas seminais somente nos grupos 2 e 7. Histologicamente observou-se desenvolvimento de hiperplasia atípica e metaplasia escamosa nos testículos e vesículas seminais e degeneração do epitélio germinativo e contração tubular nos testículos apesar do uso de probióticos. Conclusão: O uso de probiótico não protegeu o epitélio do sistema reprodutivo nesse estudo.
Wong, Pik-fan, and 黃碧芬. "The effects of castration and testosterone replacement on the gene expression of adrenomedullin and its receptor component proteins inthe rat epididymis, seminal vesicle and coagulating gland." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B42924613.
Full textBROCHARD, DENIS. "Etude structurale et fonctionnelle du promoteur du gene codant pour une proteine de secretion de la vesicule seminale de souris." Clermont-Ferrand 2, 1998. http://www.theses.fr/1998CLF22035.
Full textWong, Pik-fan. "The effects of castration and testosterone replacement on the gene expression of adrenomedullin and its receptor component proteins in the rat epididymis, seminal vesicle and coagulating gland." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B42924613.
Full textGUILBAUD, CECILE. "Etude de l'expression et de la regulation au cours du developpement de proteines androgeno-dependantes de la vesicule seminale de souris." Clermont-Ferrand 2, 1994. http://www.theses.fr/1994CLF21639.
Full textNORMAND, THIERRY. "Etude experimentale du role des androgenes dans l'expression des proteines de la vesicule seminale de souris a l'age et au cours du developpement." Clermont-Ferrand 2, 1991. http://www.theses.fr/1991CLF21252.
Full textChaulin, Bertrand. "Imagerie par résonance magnétique dans le bilan d'extension du cancer de la prostate aux vésicules séminales." Bordeaux 2, 1991. http://www.theses.fr/1991BOR23003.
Full textSIMON, ANNE-MARIE. "Etude de l'expression au cours du developpement et a l'age adulte de deux arnm androgeno-dependants de la vesicule seminale de souris. Isolement et caracterisation d'un des genes correspondant : msvsp99." Clermont-Ferrand 2, 1995. http://www.theses.fr/1995CLF21734.
Full textCAI, WEI-BO, and 蔡偉博. "Characterization of seminal vesicles autoantigen (SVA)." Thesis, 1991. http://ndltd.ncl.edu.tw/handle/03428763624300790619.
Full textHwang, Yan-Hwa, and 黃彥華. "Biochemical Characterization of the Mouse Seminal Vesicles Autoantigen (SVA)." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/3aer8f.
Full textChing-wei, Luo, and 羅清維. "Biochemical Characterization of the Mouse Seminal Vesicles Autoantigen (SVA)." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/80056083017870657226.
Full text國立臺灣大學
生化科學研究所
82
Mouse seminal vesicle autotigen (SVA) has been identified recently from our laborary. It is a 19 kDa glycoprotein and the primary structure of tis protein core has been established by cDNA cloning and proyein sequening. The main task fo this work is to study the structure and the function of SVA. The protein was estimated to contain 25 % beta-structure and no helical structure on the basis of its CD. The complex formation of SVA and zinc ion caused no change in the protein conformation. The carbohydrate moeity of SVA composed of galactose, (L)-fucose, mannose,glucose,N-acetylglucosamine,NGNA, NANA. The results of lectin-glycoprotein agglutination test suggested galactose,(L)- fucose as terminal residues of the conjugated carbohydrate. SVA wae not detectable with antiserum angainst phosphoserine, phosphothreonine ,phosphotyrosine ,nor was phosphorylated by protein kinase C and endogeneouskinase. This revealed that SVA is not a phosphoprotein.The results of immunolocation and Western blotting showed that SVA could bind specifically on the midpiece of sperm.
CHEN, JIN-LONG, and 陳金龍. "Autoimmunization and isoimmunization of mouse seminal vesicle secretion:isolation and characterization of seminal vesicle autoantigens (SVA)." Thesis, 1990. http://ndltd.ncl.edu.tw/handle/17327474122678963264.
Full textWANG, BEN-NEN, and 王本甯. "Studies of Mouse Seminal Vesicle Autoantigen Promoter." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/54964076007150367955.
Full text國立臺灣大學
生化科學研究所
86
Semen comes mainly from the secretions of seminal vesicle and other accessory glands. An autoantigen，the seminal vesicle autoantigen (SVA)，has been purified from seminal vesicle secretion. It is a 19 kDa androgen-dependent glycoprotein. The preliminary data from our laboratory indicate that SVA is a sperm mobility inhibitor with the ability to suppress the BSA-induce sperm capacitation. By comparison，ten potential androgen response elements (AREs) have been mapped on the gene. In this work，we ligated different fragments of SVA promoter and intronic regions on the upstream of a reporter gene，chloramphenicol acetyltransferase (CAT). Eight plasmids were constructed and their transcriptional activities were evaluated by CAT assay. Including ARE-a，ARE-b，and TATA-box together，the plasmid could enhance 2.5 folds of CAT expression under induced by 10 nM R1881. Under the same condition，ARE-c，ARE-d，and TATA-box were cooperated to enhance 4.8 folds of CAT expression. Our preliminary study suggested that the protein(s) from nuclear extract of seminal vesicle could bind to -250~+8 region of SVA gene.
Yu, Long-Chih, and 余榮熾. "Biochemical Study of Mouse Seminal Vesicle Autoantigen." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/95043587554996347992.
Full text國立臺灣大學
生化科學研究所
82
A protein extract of mouse seminal vesicle secretion was used to immunize mature mice (BALB/c) of both sexes. Results of Western analyses for these secretory proteins indicated that only one minor protein component could be recognized by the autoantisera prepared from either autoimmunization of male mice or isoimmunization of female mice. The autoantigen was purified from seminal vesicle secretion. The autoantigen has glycoprotein characteristics: the majority of carbohydrate is N- linked and the remainder is O-linked carbohydrate. Rabbit antibodies to the autoantigen were used to isolate the corresponding cDNA from a mouse seminal vesicle cDNA library. The primary structure deduced from the cDNA sequence was confirmed with direct amino acid sequence determination. The results indicated that the core protein consists of 131 amino acid residues. The core protein and the carbohydrate portion together have a molecular weight of 19 KDa. Analysis of the primary structure indicated that the autoantigen has two potential sites for the N-linked carbohydrate at Asn-12 and Asn-122. Results of Western and Northern analyses for various tissues indicated that the seminal vesicle is the sole organ to produce this autoantigen. The gene expression of this autoantigen was stimulated by testosterone. When the autoantibody been induced in the mice, the fertility tended to decrease but the significance was not prominant. An genomic fragment of this autoantigen was isolated from a mouse genomic library. Analysis of the partial sequence of the 5''-flanking region of this DNA fragment suggested the existence of two potential androgen response elements. These two elements exhibited the binding ability to a specific nuclear protein in mouse seminal vesicles.
CHEN, XUAN-FEI, and 陳璿妃. "Studies on seretory protease inhibitors of mouse seminal vesicle." Thesis, 1989. http://ndltd.ncl.edu.tw/handle/54070237151370028940.
Full textYa-Ling, Hsiao, and 蕭雅鈴. "The Genomic Structure of A Mouse Seminal Vesicle Autoantigen." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/93844826902645080667.
Full textShu, Jye-An, and 徐捷安. "Regulation Mechanism of Seminal Vesicle Autoantigen on Mouse Sperm Capacitation." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/42056614802740899594.
Full text臺北醫學大學
醫學研究所
96
Capacitation processes are complex biological events for sperm to aquire the ability for fertilization. It was tightly regulated by capacitation factors and decapacitation factors. Many attempts have been shown to demonstrate the capacitation mechanisms; however, the decapacitation regulation remains unclear. In the past ten years, our lab has been demonstrated a seminal vesicle autoantigen (SVA), a novel 19 kDa phospholipids-binding protein in seminal plasma, could play a role as a decapacitation factor to suppress the capacitation factor (like BSA, PAF, and cyclodextran)-induced mouse sperm capacitation. The mechanism of SVA suppression including decrease of the intracellular calcium and cAMP levels, capacitation-associated protein tyrosine phosphorylation, sperm motility, and acrosome reaction. In addition, SVA also enhanced the Ca2+ efflux through activation of the plasma membrane Ca2+-ATPase (PMCA) activity, and, decreased the mitochondria membrane potential of sperm cells. Given SVA interacts with sphingomyelin (SPM) which is a major component in lipid raft, we therefore tried to exam the interaction of SVA and lipid raft on sperm membrane. In our results, we found that SVA is able to suppress the cyclodextran-induced capacitation and its-associated protein tyrosine phosphorylation in sperm. However, by using GM1 and caveolin as markers, we could not observe the inhibition effect of SVA on the BSA-induced distribution pattern of lipid-raft on sperm membrane. This result is further supported by the cell model of cyclodextran-TGF-beta induced PAI-1 gene expression. In addition, SVA significantly suppressed the sAC activity of mouse sperm. In summary, SVA may interact with non-lipid raft region on sperm membrane, by which to suppress the sAC and intracellular signals. As how SVA signal pathway through non-lipid raft region to downstream molecular events still requires further studies.
KUO, SHIN-PEI, and 郭欣珮. "Decapacitation Mechanism of Sperm Induced by Mouse Seminal Vesicle Autoantigen." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/60177925994152174664.
Full text臺北醫學大學
醫學研究所
92
Sperm capacitation involves a complex biological molecular event to acquire the capacity for acrosome reaction and fertilization. During the transit of sperm from male testis to female oviduct, capacitation should take place at right time and right place in the reproductive tract after ejaculation. Factors promoted or inhibited sperm activity should interplay to prevent the gamete prematuriza- tion. Recently, the molecular events associated with capacitation are well documented, but less progress has been made to study the decapacitation effect. Here, we demonstrated a seminal vesicle autoantigen (SVA), a novel 19kDa phospholipids-binding protein, which serves as a decapacitation factor to suppress the mouse sperm capacitation induced by BSA / PAF, by reducing intracellular pH value, calcium ion concentration, and cAMP concentration. These data implicates the involvement of cAMP-dependent pathway in the decapacitation effect caused by SVA. Moreover, we found that the SVA effected the descending of mitochondria membrane potential as detected by Mitotracker- red feeding. The relevance of this observation to the SVA ability in the suppression of sperm motility awaits further study.
CHEN, XI-DI, and 陳希迪. "Anandrogen-dependent Kazal type trypsin inhibitor from mouse seminal vesicle." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/86184828961370258874.
Full textTSENG, HUAN-CHIN, and 曾煥清. "Study of mouse SVS I protein in mouse seminal vesicle secretion." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/50863159613582794136.
Full text國立臺灣大學
生化科學研究所
91
The genomic structure of MpSv-2, a novel androgen-regulated gene exclusively expressed in mouse seminal vesicle, was analyzed to establish a 5-flanking region of 2002 bp, five exons of 2038、277、134、139 and 133 bp and 4 introns of 1060、278、240 and 509 bp.The length of MpSv-2 cDNA is 2733bp.The putative MpSv-2 translate protein contains 820 amino aids that sum to give a molecular mass of 90,200 Da. The translate protein was predicted to a secretory protein and the molecular mass of MpSv-2 translate protein is similar to mouse seminal vesicle secretion protein SVS I. Trypsin digestion of SVS I and MALDI-TOF mass spectral analysis supported this protein as derived from MpSv-2.SVS I was immunolocalized to both the epithelium of the primary and secondary folds of the seminal vesicle.All of mouse SVS I-III were proven to be substrates of transglutaminase and could be cross-linked readily after the enzyme reaction.
Guo, Hong-Wen, and 郭鴻文. "Physiological Study of a Trypsin Inhibitor from Mouse Seminal Vesicle Secretion." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/18323136940431232478.
Full text國立臺灣大學
生化科學研究所
86
A kazal-type trypsin inhibitor (TI) has been previously purified from seminal vesicle secretion in our laboratory. The protein contains no carbohydrate and its molecular weight is 6.4 kDa as estimated by SDS-electrophoresis. Among the reproductive tracts of mice, TI is product only male accessory sexual gland such as seminal vesicles, prostate and coagulating gland. This work aimed to assess the effect of TI on sperm activity. Moreover, TI could suppressed the bovine secretion serum albumin induced sperm capacitation and acrosome reaction as lecular weig assayed by the chlortetracycline (CTC) fluorescence method and the uctive trac Coomassie Blue (CB) staining technique. A protein with a Mr around seminal ves 15 kDa could be isolated from the supernatant of frozen-thawed s the effect of epididymal sperm suspension through an affinity column of agrose erum albumin linked with TI. acitation and acrosome reaction as assayed by the chlortetrac
Luo, Ching-Wei, and 羅清維. "Structure and Function of SVS VII Secreted from Mouse Seminal Vesicle." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/49316805019095828141.
Full text國立臺灣大學
生化科學研究所
89
Mouse seminal vesicle secretion contains seven well-defined major proteins designated SVS I-VII in decreasing order of molecular weight. We used various proteases to carry out proteolysis of the protein components of mouse SVS after their resolution in SDS/PAGE copolymerized with gelatin. Only SVS VII was detected in the gel. SVS VII was purified to homogeneity by chromatography and its antiserum was prepared. The primary structure was established using cDNA cloning and confirmed by protein sequencing. Accordingly, it has a theoretical molecular mass of 8538, which was proven by the electrospray mass spectrum. SVS VII consists of 76 amino acid residues in which 10 cysteines are crosslinked into five disulfide bonds. The CD spectrum indicated that the structure of SVS VII was stable over a wide range of pH values but labile after heating. Result of Western and Northern blot analyses for various tissues indicated that SVS VII protein and its RNA message are expressed only in the seminal vesicle. SVS VII was immunolocalized in luminal epithelium of seminal vesicle. The developmental profile of SVS VII expression in the accessory gland showed no positive correlation with the animal age. Even in the animals that had been castrated, SVS VII and its mRNA remained detectable in the seminal vesicles. A cytochemical study illustrated the presence of the SVS VII-binding region on the entire surface of mouse sperm. We found that the SVS VII-sperm binding was influenced by the salt concentration in the culture medium. The data from binding assay at physiological condition showed that there were two types of SVS VII-binding sites on sperm surface, a high-affinity site with Ka of 5.2x10-6 M-1 and a low-affinity site with Ka of 1.5x10-6 M-1. The SVS VII-sperm binding was inhibited by the dispersed sperm lipids, suggesting the binding sites on spermatozoa are lipid in nature. The results of TLC overlay assay for the binding of 125I-SVS VII to phospholipids and the interaction between SVS VII and phospholipid liposomes demonstrated specific binding of this protein to both phosphatidylethanolamine and phosphatidylserine. The SVS VII-sperm binding greatly enhanced the sperm motility but did not induce the sperm capacitation. SVS VII after heat treatment could enhance sperm motility initially but immobilized the sperm thereafter. After reductive alkylation, SVS VII lost the ability to enhance motility of sperm.
Yan-hwa, Lin, and 林彥輝. "Biochemical Study of a Trypsin Inhibitor from Mouse Seminal Vesicle Secretion." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/03456896046561484169.
Full text國立臺灣大學
生化科學研究所
82
A Kazal-type trypsin inhibitor from mouse seminal vesicle se- cretion was purified to homogeneity via a series of purification steps including ammonium sulfate fractionation, gel filtration, and HPLC on a reverse phase C4 column.The molecular weight of the protein was determined to be 7 kDa by both gel chromatography and SDS PAGE.The protein contained no carbohydrate. Results of direct amino acid sequence determination indicated this protein was the product of MP12 cDNA identified from mouse prostate . By recombinant DNA manipulation,E.coli cells were transformed with an expression vector constructed by inserting the DNA se- quence coding for the inhibitor into pGEX-2.The cloned cells were able to produce high yield of a chimeric polypeptide made by fu- sing the trypsin inhibitor to glutathione-S-transferase.The anti- serum was used for the Western blot procedure to examine tissue distribution of MSVS TI among reproductive tracts. MSVS TI was found only in prostate glands and seminal vesicles. The results were confirmed by Northern blot analysis.Production of MSVS TI in seminal vesicles increased with the growth of the organ and could be stimulated by testosterone. Results of fluorescence immunolocalization demonstrated a specific binding of MSVS TI on the acrosome region of sperm head.
Yang, Yun-Hsin, and 楊允馨. "Preliminary study of the gene structure of mouse seminal vesicle autoantigen." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/14603954992729095722.
Full text國立臺灣大學
生化科學研究所
82
From the analysis of a genomic clone screened from mouse DNA Library, 1016-bp of SVA gene was established. They covered the first exon (122bp), part of the first intron(172bp) and the upstream 722bp from transcriptional start site. Sequence analysis revealed two putative AREs as follows:one between position -124 and -110 (element A, EA) and the other between -213 and -199 (element B, EB)。 EA (AGAACAaagAGTGTG) and EB( AGAACAttcTAATCT) have 66.7% and 83.3% sequence homology with the consensus ARE (AGAACAnnnTGTTCT). Nuclear protein extract of seminal vesicle was able to retard the synthetic EA or EB as evident from mobility shift DNA assay. The interaction between either elements and nuclear protein was specific and EB showed stronger than EA in its affinity. In order to test if EA or EB is able to act as an androgen- dependent transcriptional enhancer, series of SVA 5'-flanking fragments were ligated to pCAT-Basic while EA or EB fragment was inserted into pCAT- Promoter. The constructed CAT-fusion plasmids could be introduced into LNCap cells to examine the androgenic inducibility of transcriptional activity. The systems of cell culure, transfection and CAT assay have been successfully established. Future work will be the test of these constructed CAT-fusion plasmids to obtain more insight into the mechanism of androgen action on SVA.
Lai, Min-Long, and 賴明龍. "Biochemical study of a kazal type trypsin inhibitor from mouse seminal vesicle secretion." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/03063349080939818375.
Full text國立臺灣大學
生化科學研究所
81
A Kazal-type trypsin inhibitor purified from mouse seminal vesicle secretion. It was shown to be a weak basic protein with an isoelectric point of 8.7 and it contained no carbohydrate. The protein had a specific activity of 184 U/ miligram protein. Analysis of the kinetic data revealed that the protein was a competitive inhibitor with an inhibitory constant (Ki) of 0.15 nana Mol. The molcular mass of the protein was determined to be 7000. Results of direct amino acid sequence determinations indicated that this protein corresponded to the reading frame of MP12 cDNA manipulated from mouse prostate (Mills et al., 1987a). E. coli cells were transformed with an expression vector constructed by inserting the DNA sequence encoded for this inhibitor into pGEX-2. The chimeric polypeptide could be partially purified, was digested further with thrombin and a recombinant trypsin inhibitor was isolated. Antibody induced from the chimeric polypeptide could recognize native inhibitor specifically. The trypsin inhibitor bound to the acrosomal cap region of sperm head which appeared as a crescent-shaped fluorescence as the cells were stained by indirect immunofluorescent technique. Immunoaggregation of the bound trypsin inhibitor on the sperm surface resulted in acrosome reaction. The trypsin inhibitor showed caltrin activity. It could suppress the calcium uptake of the capacitated spermatozoa. Cleavage at the reactive site Arg-Ile of the protein lost the inhibitory effect on trypsin activity but retained the caltrin activity even stronger than the native protein. This trypsin inhibitor may function during the early stages of sperm transit through the female reproductive tract to prevent a premature onset of the acrosome reaction by inhibition of the calcium transport prior to acrosome reaction.
Huang, Hsiu-Ni, and 黃岫妮. "Biochemical Characterization of the Mouse Seminal Vesicle Secretion Sulfhydryl Oxidase -2（SOx-2）." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/81946113468584089103.
Full text國立臺灣大學
生化科學研究所
91
Sulfhydryl Oxidase-2, SOx-2, is a 66kDa, FAD-binding , monomeric protein. It catalyzes protein disulfide bridge formation and helps unfolded proteins to be matured and biologically functional. From previous studies, SOx is expressed ubiquitously, but most strongly in secretory tissues, such as seminal vesicle, skin apocrine glands and so on.（Colin Thorpe et al.,2002）. In this thesis, we purified SOx-2 protein from mouse seminal vesicle fluid and produced anti-SOx-2 anti-serum. Tissue distributions survey of SOx-2 mRNA showed SOx-2 mRNA is expressed extremely highly in seminal vesicle, but in an androgen-independent manner. From immunohistochemistry experiment focused on seminal vesicle, we found that SOx-2 protein is distributed in the epical region of the mucosa fold columnal cells，which is a typical pattern of secretory proteins. Moreover, using LCM（laser capture microdissection）technique, we proved that SOx-2 mRNA is expressed in the mucosa fold cells but not smooth muscle cells. In the sperm capacitation assay, we showed that exogenous SOx-2 protein exhibited inhibitory effects on sperm capacitation. In this thesis, we did the SOx-2 protein enzyme activity assay and revealed that SOx-2 protein catalyzes disulfide bridge formation. From a primary test of crude SVS（seminal vesicle secretion）on SDS-PAGE, we can see in the reductant-treated SVS the high molecular weight polymer had been broken down into the low molecular weight monomers. In further research, we may concentrate on whether if SOx-2 protein has the ability to crosslink the major proteins in mouse SVS to form polymer complex of copulatory plug.
林玲宜. "Biochemical study of a recombinant kazal type trypsin inhibitor from mouse seminal vesicle secretion." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/86086194063145120339.
Full textLee, Chin-Mei, and 李金美. "Genetic and Functional Study of the Rat Seminal Vesicle Secretion Protein III, RSVS III." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/24250274396889114322.
Full text國立臺灣大學
生化科學研究所
92
Rat seminal vesicle secretion protein contains five well-defined major components designated RSVS I-V in decreasing order of molecular weight. RSVS I-III were covalent cross-linked to form high molecular complexes by transglutaminase. RSVS III was confirmed to be derived from RpSV-1 mRNA. RSVS III was immunolocalized in the luminal epithelium of seminal vesicle and the copulatory plug. The genomic structure of RpSv-1(RSVS IIIα)was analyzed to establish a 5’-flanking region of 1771 bp, three exons of 92, 1482 and 327 bp, and two introns of 230 and 89 bp. The transcription unit is organized with the first exon encoding a signal peptide, and the second exon encoding a protein in its entirety, whereas the third encompasses a 3’-untranslated region, that shares common features with the arrangement of transcription units of the rapid evolving substrate of tansglutaminase (REST) gene family. The RSVS IIIα gene was mapped to chromosome 3q42 locus. We found another paralog, RSVS IIIβ gene, which shares 99.9% nucleotide sequence identity to RSVS IIIα in the same locus. Northern blotting and RT-PCR were performed to demonstrate the exclusive expression of this duplicated pair in seminal vesicle with a ratio of RSVS IIIα mRNA to RSVS IIIβ mRNA ≒ 2.0. The mRNA expression of RSVS IIIα/ RSVS IIIβ were stimulated by testosterone. The genes in human chromosome 20q13, mice chromosome 2H3 and rat chromosome 3q42 were identified to have the arrangement of their transcription units similar to a REST gene. The molecular evolution of these REST genes is discussed.