Dissertations / Theses on the topic 'Seed enhance'
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Kornacki, Catherine. "MANIPULATING OIL SEED BIOCHEMISTRY TO ENHANCE THE PRODUCTION OF ACETYL-TAGS." Thesis, Kansas State University, 2016. http://hdl.handle.net/2097/35753.
Full textBiochemistry and Molecular Biophysics Interdepartmental Program
Timothy P. Durrett
Using vegetable oils directly as an alternative biofuel presents several problems as such oils typically possess poor fuel qualities including high viscosity, low volatility, and poor cold temperature properties. The ornamental shrub Euonymus alatus produces unusual acetyl-1,2-diacyl-sn-glycerols (acetyl-TAGs) that have an acetyl group in the sn-3 position instead of a long chain fatty acid. The presence of this sn-3 acetyl-group give acetyl-TAGs properties desirable for biofuels, such as reduced viscosity, comparted to the normal long chain triacyglycerols found in most vegetable oils. Acetyl-TAGs are synthesized by the Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) and Euonymus fortunei diacylglycerol acetyltransferase (EfDAcT) enzymes. Both enzymes catalyze the transfer of an acetyl group from acetyl-CoA to diaclglycerol (DAG) to produce acetyl-TAGs. Previous work demonstrated that expression of EaDAcT combined with the suppression of a diacylglycerol aceyltransferase (DGAT1) in Camelina sativa led to seeds with 85 mol % acetyl-TAGs. Increasing acetyl-TAG levels further was explored using two strategies. Over expression of citrate lyase to increase the pool of acetyl-CoA to be used as a substrate for the acetyltransferase enzymes failed to increased levels of acetyl-TAGs. A second approach involved expressing EfDAcT in Camelina sativa. EfDAcT has demonstrated higher activity in vitro and in vivo and its expression in yeast leads to approximately 50 % higher levels of acetyl-TAGs compared to EaDAcT. The expression of EfDAcT coupled with the suppression of DGAT1 in Camelina sativa resulted in 90 mol % acetyl-TAGs in the transgenic seeds. Levels of EfDAcT protein analyzed in developing transgenic Camelina sativa seeds across a 40 day time period were highest at 15 and 20 days after flowering. Following these time points acetyl-TAG accumulation increased rapidly, coinciding with the higher enzyme expression levels. The optimization of additional promoters to ensure expression of EfDAcT in the last half of seed development could represent another way to further increase acetyl-TAGs in the future.
Brits, Yvette. "A comparison of selected enhanced (coated) and non-enhanced grass seed types for re-seeding of disturbed areas / Yvette Brits." Thesis, North-West University, 2007. http://hdl.handle.net/10394/4252.
Full textThesis (M.Sc. (Environmental Science and Management))--North-West University, Potchefstroom Campus, 2008.
McNickle, Cathy, and n/a. "Enhanced access for re-entry into education, training is is seen to enable women to achieve self-fulfilment and to enhance employment opportunities." University of Canberra. Education, 1994. http://erl.canberra.edu.au./public/adt-AUC20061013.143535.
Full textShanor, C., T. Ensley, D. J. Hagan, Stryland E. W. Van, E. M. Wright, and M. Kolesik. "Numerical investigation of enhanced femtosecond supercontinuum via a weak seed in noble gases." OPTICAL SOC AMER, 2016. http://hdl.handle.net/10150/621524.
Full textMunder, Simon Manuel [Verfasser]. "Plant oil from sunflower seeds - Process modelling and optimisation to enhance quality / Simon Manuel Munder." Aachen : Shaker, 2018. http://d-nb.info/1188549472/34.
Full textMunder, Simon [Verfasser]. "Plant oil from sunflower seeds - Process modelling and optimisation to enhance quality / Simon Manuel Munder." Aachen : Shaker, 2018. http://nbn-resolving.de/urn:nbn:de:101:1-2019061605390395479412.
Full textIhemere, Uzoma Enyinnaya. "Somatic embryogenesis and transformation of cassava for enhanced starch production." Connect to this title online, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1070549008.
Full textTitle from first page of PDF file. Document formatted into pages; contains xxiii, 184 p.; also includes graphics (some color). Includes bibliographical references (p. 166-184).
Sanci, Rukiye. "Synthesis Of Colloidal Silver Particles With Different Sizes By Seeding Approach For Surface Enhanced Raman Scattering (sers) Studies." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/3/12611076/index.pdf.
Full textLebenius, Filippa, Ida Andersson, and Filippa Skoglund. "See what I'm saying? : A qualitative study of how sensory stimulation enhances the customer shopping experience in e-commerce." Thesis, Linnéuniversitetet, Institutionen för marknadsföring (MF), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-44618.
Full textGriffiths, Thomas Richard. "An Enhanced Data Model and Tools for Analysis and Visualization of Levee Simulations." Diss., CLICK HERE for online access, 2010. http://contentdm.lib.byu.edu/ETD/image/etd3477.pdf.
Full textRoussille, Ludovic. "Suivi quantitatif in situ d'interactions biomoléculaire par microscopie optique SEEC." Thesis, Le Mans, 2012. http://www.theses.fr/2012LEMA1030/document.
Full textThis thesis was supported by National Agency for Research with the project: ANR PNANO-07 SEEC. The Surface Enhanced Ellipsometric Contrast (SEEC) microscopy has invented in 2000 at Le Mans (France). This technique allows the visualization of nanoscopic object between crossed analyzer and polarizer. It’s possible if some special multilayer surfaces are used. There surfaces must have the particularity to not change the polarization of light during the reflection. Until the beginning of the project the SEEC microscopy was useful only for air observations. The goal of the thesis was to adapt this technique to observe on gold surfaces immerged in water and to compare the performance of the SEEC microscopy with Surface Plasmonique Resonance (SPR) in that configuration. The SPR is a biomolecular interaction study reference technique. SEEC microscopy lateral resolution was evaluate by fluorescence microscopy. Next, we realize two model experiments monitor in parallel by SEEC microscopy and by SPR: BSA immobilization and biotinylated IgG fixation by immobilized streptavidine. To compare measurements efficiently we did a huge preparation work (surface functionalizations and microfluidic) to have exactly same conditions in both techniques.Our results show SEEC microscopy cannot replace SPR for biomolecular interaction studies but it can be used as cheap immunological diagnostic technique. This work gives the path to follow on that direction
Factor, Daniel C. "Understanding Epigenetic Controllers of Stem Cell Fate and Function." Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1504285124759387.
Full textZubrickaitė, Rasa. "Optimalios gelio su kaštonų sėklų ekstraktu sudėties paieška, technologija ir vertinimas." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20110628_151417-94943.
Full textChronic venous insufficiency (CVI) describes a condition that affects the venous system of the lower extremities with venous hypertension causing various pathologies including pain, swelling, edema, skin changes, and ulcerations. Saponosides from horse chestnut extract are one of four groups of venoactive drugs, that have been evaluated in the treatment of CVI to reduce symptoms and help prevent the development of secondary complications and the progression of disease. This study assessed the following: (1) the permeability in vitro through cellulose membranes of polyphenolic compounds in twenty different horse chestnut seed extract (0,5% w/w) gel formulations (Ultrez 10, Ultrez 20, Carbopol 980 and Carbopol 940 gels (1%) with different peppermint oil concentrations (0; 0,1; 0,5; 1; 1,5%), propylenglycol, isopropyl alcohol, TEA, water); (2) the viscosity of all gels and its influence for in vitro permeation. Results: As the base-gels to investigate the effect of the in vitro penetration from different carbomers was formultions without peppermint oil. Gels with Ultrez 20 had the lowest and with Carbopol 980 the highest viscosity. By increasing concentration of peppermint oil, viscosity decreased and the difference between 0% and 1,5% in all carbomers was statistically significant (p<0,05). The quantity of the released polyphenols in time function was determined by spectrophotometric method. Ultrez 20 gel (0% peppermint oil) showed the highest flux during the first half hour (... [to full text]
Tavares, Lucas Alves. "O envolvimento da proteína adaptadora 1 (AP-1) no mecanismo de regulação negativa do receptor CD4 por Nef de HIV-1." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/17/17136/tde-06012017-113215/.
Full textThe Human Immunodeficiency Virus (HIV) is the etiologic agent of Acquired Immunodeficiency Syndrome (AIDS). AIDS is a disease which has a global distribution, and it is estimated that there are currently at least 36.9 million people infected with the virus. During the replication cycle, HIV promotes several changes in the physiology of the host cell to promote their survival and enhance replication. The fast progression of HIV-1 in humans and animal models is closely linked to the function of an accessory protein Nef. Among several actions of Nef, one is the most important is the down-regulation of proteins from the immune response, such as the CD4 receptor. It is known that this action causes CD4 degradation in lysosome, but the molecular mechanisms are still incompletely understood. Nef forms a tripartite complex with the cytosolic tail of the CD4 and adapter protein 2 (AP-2) in clathrin-coated vesicles, inducing CD4 internalization and lysosome degradation. Previous research has demonstrated that CD4 target to lysosomes by Nef involves targeting of this receptor to multivesicular bodies (MVBs) pathway by an atypical mechanism because, although not need charging ubiquitination, depends on the proteins from ESCRTs (Endosomal Sorting Complexes Required for Transport) machinery and the action of Alix, an accessory protein ESCRT machinery. It has been reported that Nef interacts with subunits of AP- 1, AP-2, AP-3 complexes and Nef does not appear to interact with AP-4 and AP-5 subunits. However, the role of Nef interaction with AP-1 or AP-3 in CD4 down-regulation is poorly understood. Furthermore, AP-1, AP-2 and AP-3 are potentially heterogeneous due to the existence of multiple subunits isoforms encoded by different genes. However, there are few studies to demonstrate if the different combinations of APs isoforms are form and if they have distinct functional properties. This study aim to identify and characterize cellular factors involved on CD4 down-modulation induced by Nef from HIV-1. More specifically, this study aimed to characterize the involvement of AP-1 complex in the down-regulation of CD4 by Nef HIV-1 through the functional study of the two isoforms of ?-adaptins, AP-1 subunits. By pull-down technique, we showed that Nef is able to interact with ?2. In addition, our data from immunoblots indicated that ?2- adaptin, not ?1-adaptin, is required in Nef-mediated targeting of CD4 to lysosomes and the ?2 participation in this process is conserved by Nef from different viral strains. Furthermore, by flow cytometry assay, ?2 depletion, but not ?1 depletion, compromises the reduction of surface CD4 levels induced by Nef. Immunofluorescence microscopy analysis also revealed that ?2 depletion impairs the redistribution of CD4 by Nef to juxtanuclear region, resulting in CD4 accumulation in primary endosomes. Knockdown of ?1A, another subunit of AP-1, resulted in decreased cellular levels of ?1 and ?2 and, compromising the efficient CD4 degradation by Nef. Moreover, upon artificially stabilizing ESCRT-I in early endosomes, via overexpression of HRS, internalized CD4 accumulates in enlarged HRS-GFP positive endosomes, where co-localize with ?2. Together, the results indicate that ?2-adaptin is a molecule that is essential for CD4 targeting by Nef to ESCRT/MVB pathway, being an important protein in the endo-lysosomal system. Furthermore, the results indicate that ?-adaptins isoforms not only have different functions, but also seem to compose AP-1 complex with distinct cell functions, and only the AP-1 variant comprising ?2, but not ?1, acts in the CD4 down-regulation induced by Nef. These studies contribute to a better understanding on the molecular mechanisms involved in Nef activities, which may also help to improve the understanding of the HIV pathogenesis and the related syndrome. In addition, this work contributes with the understanding of primordial process regulation on intracellular trafficking of transmembrane proteins.
Hung, Jian-Ting, and 洪健庭. "Co-precipitation of Bitter Gourd Seed Oil and Sucrose Stearate Using Gas Saturated Solutions to Protect α-ESA and Enhance its Oxidation Resistance." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/nuh994.
Full text國立中興大學
化學工程學系所
103
In this study the method of supercritical particles from gas-saturated solution (PGSS) has been used for producing the micro-particles of bitter gourd seed oil (BGSO) coated by sucrose stearate (SS). During supercritical PGSS co-precipitation preliminary experiment, total yield, drug content of the coating have been investigated by changing the pressure, temperature, and feed ratio of BGSO and SS. And then the response surface methodology (RSM) experimental design based on the two variables of pressure (120 bar to 160 bar) and the feed ratio (0.5 to 1.5) of BGSO and SS has been implemented to investigate the impacts of the two variables on total yield, drug content, and α-eleostearic acid (α-ESA) recovery rate of the coating. The experimental results indicate the correlation of opposite trend between the total yield and drug content of the coating. The three responses predicted by RSM with optimal conditions of pressure at 120 bar, feed ratio at 1.5 are the total yield of coating at 45.96%, the drug content at 25.76%, and α-ESA recovery rate at 29.82%, all of which are similar to the experimental values. It has been revealed by the induction that the drug content of the coating has been significantly affected by the feed ratio. As for the stability test, keeping the PGSS coating in room temperature for 30 days can slow down the decline of α-ESA concentration. The bitter gourd seed oil coated by sucrose stearate produced by supercritical PGSS can indeed slow down the degradation of α-ESA, which can serve as a protection. Keywords: particles from gas-saturated solution, bitten melon, α-eleostearic acid, sucrose stearate, encapsulation
Lin, Hang, and 林涵. "Engineering cytokinin-related genes to enhance cytokinin level and using RNA interference-based gene silencing of CASBENE SYNTHASE 1 for seed detoxification in Jatropha curcas L." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/26030320614148540826.
Full text國立臺灣大學
植物科學研究所
102
Jatropha curcas L. is one of the best candidates for biodiesel production owing to high oil content of seeds. However, there are several disadvantages, such as low seed yields and phorbol esters toxicity of seed cake for feedstuff use. Previous studies indicated that cytokinin spraying was able to promote cell division, repressed apical dominance and led to increase seed yield of J. curcas. Our preliminary study revealed that exogenous application of 6-benzyladenine on inflorescence caused phenotypical changes of numerous flowers and higher seed yield. Previous studies have shown that ISOPENTENYLTRANSFERASE and CYTOKININ OXIDASE were two genes playing the major roles in controlling endogenous cytokinin level and seed yield in plants. In order to increase endogenous cytokinin level in J. curcas, we have constructed CKX5-RNAi and 35S::JcIPT1 vectors based on the spatial expression profile of JcIPTs and JcCKXs and transformed to J. curcas mediated by A. tumefaciens. Data revealed the JcIPT1 expression level in 35S::JcIPT1 transgenic line 15 is much higher than empty-vector transformed J. curcas. The 35S::JcIPT1 transgenic line 1 has higher cytokinin level than empty-vector transformed J. curcas. In parallel, the seed detoxification by engineering JcCAS1-RNAi is in progress. Casbene synthases (CASs) catalyze the first step of phorbol ester (PE) biosynthesis. PEs are the main toxic compounds in J. curcas. Our spatial expression profile of JcCAS1 indicated that JcCAS1 had higher expression level in roots and germination seeds but did not express in leaves. The expression level of JcCAS1 increased towards fruit maturity. Thus far two transgenic lines were obtained.
Loy, Gareth. "Computer vision to see people : a basis for enhanced human computer interaction." Phd thesis, 2003. http://hdl.handle.net/1885/148589.
Full textKerr, Pamela Pearl. "Guidelines for a psycho-educational programme to enhance self-actualisation of retired people." Thesis, 2002. http://hdl.handle.net/10500/1041.
Full textMbewe, Jeremiah. "Evaluation of pass-on the gift concept on the socioeconomic welfare of rural households: the case of SACHZEP and ELITE Projects in Katete District, Zambia." Diss., 2019. http://hdl.handle.net/10500/26687.
Full textDevelopment Studies
M.A. (Development Studies)