Relevant bibliographies by topics / Secretion

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Secretion'

Author: Grafiati
Published: 4 June 2021
Last updated: 31 July 2024

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Secretion.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Secretion"

1

Park, Hyung Seo, Hyeok Yil Kwon, Yun Lyul Lee, William Y. Chey, and Hyoung Jin Park. "Role of GRPergic neurons in secretin-evoked exocrine secretion in isolated rat pancreas." American Journal of Physiology-Gastrointestinal and Liver Physiology 278, no. 4 (April 1, 2000): G557—G562. http://dx.doi.org/10.1152/ajpgi.2000.278.4.g557.

Full text
Abstract:
Effects of intrapancreatic gastrin-releasing peptide (GRP)-containing neurons on secretin-induced pancreatic secretion were investigated in the totally isolated perfused rat pancreas. Electrical field stimulation (EFS) increased secretin (12 pM)-induced pancreatic secretions of fluid and amylase. EFS induced a twofold increase in GRP concentration in portal effluent, which was completely inhibited by tetrodotoxin but not modified by atropine. An anti-GRP antiserum inhibited the EFS-enhanced secretin-induced secretions of fluid and amylase by 12 and 43%, respectively, whereas a simultaneous infusion of the antiserum and atropine completely abolished them. Exogenous GRP dose-dependently increased the secretin-induced pancreatic secretion with an additive effect on fluid secretion and a potentiating effect on amylase secretion, which was not affected by atropine. In conclusion, excitation by EFS of GRPergic neurons in the isolated rat pancreas results in the release of GRP, which exerts an additive effect on fluid secretion and a potentiating effect on amylase secretion stimulated by secretin. The release and action of GRP in the rat pancreas are independent of cholinergic tone.
APA, Harvard, Vancouver, ISO, and other styles
2

Possot, Odile M., Guillaume Vignon, Natalia Bomchil, Frank Ebel, and Anthony P. Pugsley. "Multiple Interactions between Pullulanase Secreton Components Involved in Stabilization and Cytoplasmic Membrane Association of PulE." Journal of Bacteriology 182, no. 8 (April 15, 2000): 2142–52. http://dx.doi.org/10.1128/jb.182.8.2142-2152.2000.

Full text
Abstract:
ABSTRACT We report attempts to analyze interactions between components of the pullulanase (Pul) secreton (type II secretion machinery) fromKlebsiella oxytoca encoded by a multiple-copy-number plasmid in Escherichia coli. Three of the 15 Pul proteins (B, H, and N) were found to be dispensable for pullulanase secretion. The following evidence leads us to propose that PulE, PulL, and PulM form a subcomplex with which PulC and PulG interact. The integral cytoplasmic membrane protein PulL prevented proteolysis and/or aggregation of PulE and mediated its association with the cytoplasmic membrane. The cytoplasmic, N-terminal domain of PulL interacted directly with PulE, and both PulC and PulM were required to prevent proteolysis of PulL. PulM and PulL could be cross-linked as a heterodimer whose formation in a strain producing the secreton required PulG. However, PulL and PulM produced alone could also be cross-linked in a 52-kDa complex, indicating that the secreton exerts subtle effects on the interaction between PulE and PulL. Antibodies against PulM coimmunoprecipitated PulL, PulC, and PulE from detergent-solubilized cell extracts, confirming the existence of a complex containing these four proteins. Overproduction of PulG, which blocks secretion, drastically reduced the cellular levels of PulC, PulE, PulL, and PulM as well as PulD (secretin), which probably interacts with PulC. The Pul secreton components E, F, G, I, J, K, L, and M could all be replaced by the corresponding components of the Out secretons of Erwinia chrysanthemi and Erwinia carotovora, showing that they do not play a role in secretory protein recognition and secretion specificity.
APA, Harvard, Vancouver, ISO, and other styles
3

Blocker, Ariel, Pierre Gounon, Eric Larquet, Kirsten Niebuhr, Véronique Cabiaux, Claude Parsot, and Philippe Sansonetti. "The Tripartite Type III Secreton of Shigella flexneri Inserts Ipab and Ipac into Host Membranes." Journal of Cell Biology 147, no. 3 (November 1, 1999): 683–93. http://dx.doi.org/10.1083/jcb.147.3.683.

Full text
Abstract:
Bacterial type III secretion systems serve to translocate proteins into eukaryotic cells, requiring a secreton and a translocator for proteins to pass the bacterial and host membranes. We used the contact hemolytic activity of Shigella flexneri to investigate its putative translocator. Hemolysis was caused by formation of a 25-Å pore within the red blood cell (RBC) membrane. Of the five proteins secreted by Shigella upon activation of its type III secretion system, only the hydrophobic IpaB and IpaC were tightly associated with RBC membranes isolated after hemolysis. Ipa protein secretion and hemolysis were kinetically coupled processes. However, Ipa protein secretion in the immediate vicinity of RBCs was not sufficient to cause hemolysis in the absence of centrifugation. Centrifugation reduced the distance between bacterial and RBC membranes beyond a critical threshold. Electron microscopy analysis indicated that secretons were constitutively assembled at 37°C before any host contact. They were composed of three parts: (a) an external needle, (b) a neck domain, and (c) a large proximal bulb. Secreton morphology did not change upon activation of secretion. In mutants of some genes encoding the secretion machinery the organelle was absent, whereas ipaB and ipaC mutants displayed normal secretons.
APA, Harvard, Vancouver, ISO, and other styles
4

Pugsley, Anthony P., Nicolas Bayan, and Nathalie Sauvonnet. "Disulfide Bond Formation in Secreton Component PulK Provides a Possible Explanation for the Role of DsbA in Pullulanase Secretion." Journal of Bacteriology 183, no. 4 (February 15, 2001): 1312–19. http://dx.doi.org/10.1128/jb.183.4.1312-1319.2001.

Full text
Abstract:
ABSTRACT When expressed in Escherichia coli, the 15Klebsiella oxytoca pul genes that encode the so-called Pul secreton or type II secretion machinery promote pullulanase secretion and the assembly of one of the secreton components, PulG, into pili. Besides these pul genes, efficient pullulanase secretion also requires the host dsbA gene, encoding a periplasmic disulfide oxidoreductase, independently of disulfide bond formation in pullulanase itself. Two secreton components, the secretin pilot protein PulS and the minor pseudopilin PulK, were each shown to posses an intramolecular disulfide bond whose formation was catalyzed by DsbA. PulS was apparently destabilized by the absence of its disulfide bond, whereas PulK stability was not dramatically affected either by adsbA mutation or by the removal of one of its cysteines. The pullulanase secretion defect in a dsbA mutant was rectified by overproduction of PulK, indicating reduced disulfide bond formation in PulK as the major cause of the secretion defect under the conditions tested (in which PulS is probably present in considerable excess of requirements). PulG pilus formation was independent of DsbA, probably because PulK is not needed for piliation.
APA, Harvard, Vancouver, ISO, and other styles
5

Villanger, O., T. Veel, and M. G. Raeder. "Secretin causes H+ secretion from intrahepatic bile ductules by vacuolar-type H(+)-ATPase." American Journal of Physiology-Gastrointestinal and Liver Physiology 265, no. 4 (October 1, 1993): G719—G724. http://dx.doi.org/10.1152/ajpgi.1993.265.4.g719.

Full text
Abstract:
Intrahepatic bile duct epithelial cells contribute to bile formation by hormone-dependently secreting HCO3- to bile and H+ to periductular fluid. The present study was undertaken to determine whether the secretin-induced H+ secretion is due to activation of a H(+)-ATPase or Na(+)-H+ exchange. H+ secretion was estimated from the rate of intracellular pH (pHi) recovery after acid loading (24 mM NH4Cl) of microdissected bile ductules from pig liver mounted in a flow-through chamber on the stage of a microscope. pHi was measured from an estimated average of 10-15 epithelial cells using the fluorescent pHi indicator 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein and dual-wavelength excitation of fluorescence. The ducts were superfused with HCO3(-)-free N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid buffers. We found that secretin induced net H+ secretion of 4.53 +/- 0.7 mumol.ml cell volume-1 x min-1. This H+ secretion was blocked by 10(-6) M bafilomycin A1 but was unaffected by Na+ substitution with choline in the superfusion buffer. The experiments also showed that bafilomycin A1 did not block Na(+)-H+ exchange. The secretin-induced H+ secretion is probably caused by a vacuolar-type H(+)-ATPase and may constitute an important element of the cellular mechanisms causing secretin-dependent ductular HCO3- secretion into bile
APA, Harvard, Vancouver, ISO, and other styles
6

Honzawa, Norikiyo, Kei Fujimoto, Masaki Kobayashi, Daisuke Kohno, Osamu Kikuchi, Hiromi Yokota-Hashimoto, Eri Wada, et al. "Protein Kinase C (Pkc)-δ Mediates Arginine-Induced Glucagon Secretion in Pancreatic α-Cells." International Journal of Molecular Sciences 23, no. 7 (April 4, 2022): 4003. http://dx.doi.org/10.3390/ijms23074003.

Full text
Abstract:
The pathophysiology of type 2 diabetes involves insulin and glucagon. Protein kinase C (Pkc)-δ, a serine–threonine kinase, is ubiquitously expressed and involved in regulating cell death and proliferation. However, the role of Pkcδ in regulating glucagon secretion in pancreatic α-cells remains unclear. Therefore, this study aimed to elucidate the physiological role of Pkcδ in glucagon secretion from pancreatic α-cells. Glucagon secretions were investigated in Pkcδ-knockdown InR1G9 cells and pancreatic α-cell-specific Pkcδ-knockout (αPkcδKO) mice. Knockdown of Pkcδ in the glucagon-secreting cell line InR1G9 cells reduced glucagon secretion. The basic amino acid arginine enhances glucagon secretion via voltage-dependent calcium channels (VDCC). Furthermore, we showed that arginine increased Pkcδ phosphorylation at Thr505, which is critical for Pkcδ activation. Interestingly, the knockdown of Pkcδ in InR1G9 cells reduced arginine-induced glucagon secretion. Moreover, arginine-induced glucagon secretions were decreased in αPkcδKO mice and islets from αPkcδKO mice. Pkcδ is essential for arginine-induced glucagon secretion in pancreatic α-cells. Therefore, this study may contribute to the elucidation of the molecular mechanism of amino acid-induced glucagon secretion and the development of novel antidiabetic drugs targeting Pkcδ and glucagon.
APA, Harvard, Vancouver, ISO, and other styles
7

Frank, Steven A. "Microbial secretor–cheater dynamics." Philosophical Transactions of the Royal Society B: Biological Sciences 365, no. 1552 (August 27, 2010): 2515–22. http://dx.doi.org/10.1098/rstb.2010.0003.

Full text
Abstract:
Microbial secretions manipulate the environment and communicate information to neighbours. The secretions of an individual microbe typically act externally and benefit all members of the local group. Secreting imposes a cost in terms of growth, so that cheaters that do not secrete gain by sharing the benefits without paying the costs. Cheaters have been observed in several experimental and natural settings. Given that cheaters grow faster than secretors when in direct competition, what maintains the widely observed patterns of secretion? Recent theory has emphasized the genetic structure of populations, in which secretors tend to associate spatially with other secretors, reducing direct competition and allowing highly secreting groups to share mutual benefits. Such kin selection can be a powerful force favouring cooperative traits. Here, I argue that, although kin selection is a factor, the combination of mutation and demographic processes dominate in determining the relative fitness of secretors versus cheaters when measured over the full cycle of microbial life history. Key demographic factors include the local density of microbes at which secretion significantly alters the environment, the extent to which secretion enhances microbial growth and maximum local density, and the ways in which secretion alters colony survival and dispersal.
APA, Harvard, Vancouver, ISO, and other styles
8

Park, Hyung Seo, and Hyoung Jin Park. "Effects of γ-aminobutyric acid on secretagogue-induced exocrine secretion of isolated, perfused rat pancreas." American Journal of Physiology-Gastrointestinal and Liver Physiology 279, no. 4 (October 1, 2000): G677—G682. http://dx.doi.org/10.1152/ajpgi.2000.279.4.g677.

Full text
Abstract:
Because GABA and its related enzymes have been determined in β-cells of pancreas islets, effects of GABA on pancreatic exocrine secretion were investigated in the isolated, perfused rat pancreas. GABA, given intra-arterially at concentrations of 3, 10, 30, and 100 μM, did not exert any influence on spontaneous or secretin (12 pM)-induced pancreatic exocrine secretion. However, GABA further elevated CCK (10 pM)-, gastrin-releasing peptide (100 pM)-, or electrical field stimulation-induced pancreatic secretions of fluid and amylase dose dependently. The GABA (30 μM)-enhanced CCK-induced pancreatic secretions were completely blocked by bicuculline (10 μM), a GABAA receptor antagonist, but were not affected by saclofen (10 μM), a GABAB receptor antagonist. The enhancing effects of GABA (30 μM) on CCK-induced pancreatic secretions were not changed by tetrodotoxin (1 μM) but were partially reduced by cyclo-(7-aminoheptanonyl-Phe-d-Trp-Lys-Thr[BZL]) (10 nM), a somatostatin antagonist. In conclusion, GABA enhances pancreatic exocrine secretion induced by secretagogues, which predominantly induce enzyme secretion, via GABAA receptors in the rat pancreas. The enhancing effect of GABA is partially mediated by inhibition of islet somatostatin release.
APA, Harvard, Vancouver, ISO, and other styles
9

Solomon, Travis E., John H. Walsh, Louis Bussjaeger, Yumei Zong, James W. Hamilton, F. J. Ho, Terry D. Lee, and Joseph R. Reeve. "COOH-terminally extended secretins are potent stimulants of pancreatic secretion." American Journal of Physiology-Gastrointestinal and Liver Physiology 276, no. 4 (April 1, 1999): G808—G816. http://dx.doi.org/10.1152/ajpgi.1999.276.4.g808.

Full text
Abstract:
Posttranslational processing of preprosecretin generates several COOH-terminally extended forms of secretin and α-carboxyl amidated secretin. We used synthetic canine secretin analogs with COOH-terminal -amide, -Gly, or -Gly-Lys-Arg to examine the effects of COOH-terminal extensions of secretin on bioactivity and detection in RIA. Synthetic products were purified by reverse-phase and ion-exchange HPLC and characterized by reverse-phase isocratic HPLC and amino acid, sequence, and mass spectral analyses. Secretin and secretin-Gly were noted to coelute during reverse-phase HPLC. In RIA using eight different antisera raised against amidated secretin, COOH-terminally extended secretins had little or no cross-reactivity. Bioactivity was assessed by measuring pancreatic responses in anesthetized rats. Amidated canine and porcine secretins were equipotent. Secretin-Gly and secretin-Gly-Lys-Arg had potencies of 81 ± 9% ( P > 0.05) and 176 ± 13% ( P < 0.01), respectively, compared with amidated secretin, and the response to secretin-Gly-Lys-Arg lasted significantly longer. These data demonstrate that 1) amidated secretin and secretin-Gly are not separable under some chromatographic conditions, 2) current RIA may not detect bioactive COOH-terminally extended forms of secretin in tissue extracts or blood, and 3) the secretin receptor mediating stimulation of pancreatic secretion recognizes both amidated and COOH-terminally extended secretins.
APA, Harvard, Vancouver, ISO, and other styles
10

Deng, Xiaoying, Dulce R. Guarita, Martha R. A. Pedroso, Christianna Kreiss, Paul G. Wood, Alan F. Sved, and David C. Whitcomb. "PYY inhibits CCK-stimulated pancreatic secretion through the area postrema in unanesthetized rats." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 281, no. 2 (August 1, 2001): R645—R653. http://dx.doi.org/10.1152/ajpregu.2001.281.2.r645.

Full text
Abstract:
Peptide YY (PYY) inhibits CCK-8-secretin-stimulated pancreatic secretion in vivo. To investigate whether CCK-8-secretin-stimulated pancreatic secretion is mediated through a vago-vagal pathway and whether PYY inhibits this pathway through the area postrema (AP), chronic pancreatic, biliary, and duodenal catheters were implanted in AP-lesioned (APX) or sham-operated rats. The effects of APX on pancreatic secretion stimulated by bethanechol, pancreatic juice diversion (PJD), or CCK-8-secretin, were tested, with and without background PYY infusion, in unanesthetized rats. APX reduced basal pancreatic secretion by 15–20% ( P < 0.01). APX had no effect on bethanechol-stimulated secretion and potentiated protein secretion stimulated by PJD (396 vs. 284%) and exogenous CCK-8-secretin. In sham-operated rats, background PYY potently inhibited CCK-8-secretin-stimulated pancreatic fluid (1.8 vs. 48.2%) and protein secretion (3.7 vs. 45.8%) but potentiated fluid (52.9 vs. 43.1%) and protein (132.9 vs. 68.9%) secretion in APX rats. Our findings demonstrate that PYY inhibits CCK-8-secretin-stimulated pancreatic secretion through an AP-dependent mechanism in sham-operated rats. The AP also contributes to basal pancreatic secretion.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Secretion"

1

Gu, Shuang. "Secretin interactions in the type II secretion system." Thesis, Queen Mary, University of London, 2012. http://qmro.qmul.ac.uk/xmlui/handle/123456789/2482.

Full text
Abstract:
The type II secretion system (T2SS) is the major terminal branch of the general secretory pathway. It is composed of 12-15 proteins, most in multiple copies, and spans the inner and outer membranes of Gram-negative bacteria. The T2SS secretin subunits form a large dodecameric torus-like structure in the outer membrane. The secretin is the only essential component in the outer membrane and secreted proteins and virulence factors pass through the pore in the toroidal secretin dodecamer and out into the environment. The interaction between the secretin and its partners plays a key role in regulation of the T2SS. The interaction between the so-called homology region of the innermembrane protein GspC (GspC-HR) and secretin provides the structural and functional integrity of the secretion machinery across the two cell membranes. The interaction between secretin and its pilotin translocates the secretin subunits to the outer membrane. In this Thesis, the interactions between secretin and its partners are studied at molecular level. The GspC-HR structure is solved using NMR spectroscopy. Its interaction with secretin (GspD) is elucidated using several biochemical and biophysical approaches and a model of the complex is proposed. Also, the interaction between secretin (GspD) and pilotin (GspS) is further charicterisied. An 18 residues secretin sequence is identified as responsible for interacting with pilotin. Upon binding to the pilotin, the unstructured secretin forms a helical structure.
APA, Harvard, Vancouver, ISO, and other styles
2

Woods, Birgitta A. "The effects of epinephrine, AVP, norepinephrine, and acetylcholine on lung liquid production in in vitro preparations of lungs from fetal guinea pigs (Cavia porcellus)." Thesis, University of British Columbia, 1991. http://hdl.handle.net/2429/29821.

Full text
Abstract:
This study examined the effects of epinephrine, norepinephrine, AVP and ACh on fluid movement by the lungs of the late-term guinea pig fetus. Catecholamines and AVP are secreted in high amounts by the fetus during delivery, and could be important with respect to fetal lung fluid removal; this event is vital at the time of birth. The lungs were supported in vitro for a duration of three hours, and production rates were measured using a dye-dilution technique. The average resting production rate in terms of ml/kg‧h declined with gestational age (54-67 days gestation; n=171). There was a lesser decline in the average resting production rate in terms of ml/h. The average production rate of untreated preparations in the first hour was 1.60 ± 0.26 ml/kg body weight per hour, and rates did not change significantly during the remaining two hours of experimentation (n=30). This rate is comparable to those reported from chronically catheterized fetal sheep. Treatment was administered during the second hour of experimentation, following an ABA design. Lungs (n=36) were transferred to fresh Krebs-Henseleit saline containing one of the following concentrations of epinephrine: (a) 10‾⁵ M; (b) 10‾⁶ M; (c) 10‾⁷ M; (d) 5 x 10‾⁸ M; (e) 10‾⁸ M; and (f) 10‾⁹ M. With the exception of the top dose, epinephrine treatment caused an immediate reduction in fluid secretion, or fluid reabsorption. Sodium followed the movement of water in all cases. The effect of epinephrine at 10‾⁷ M was maximal, and the threshold dose for epinephrine was calculated at 1.78 x 10‾¹¹ M. Phentolamine and propranolol had no effect in control preparations. However, phentolamine completely blocked the effect of epinephrine, whereas propranolol was ineffective. Isoproterenol had no effect on pulmonary fluid production. Alpha-adrenergic receptors apparently mediate the effect of epinephrine on pulmonary fluid movement in the fetal guinea pig lung. This conclusion is different from that obtained in fetal sheep, in which beta-adrenergic receptors are utilized. A possible synergism between epinephrine and AVP was examined. Lungs (n=12) were transferred to fresh Krebs-Henseleit saline containing either (a) 0.6 mU/ml AVP, or b) 0.6 mU/ml AVP combined with epinephrine at 10‾⁷ M. Treatment with AVP caused a slow, prolonged reduction in fluid production. Treatment with AVP together with epinephrine did not demonstrate synergism. The effect of norepinephrine (NE) was examined. Lungs (n=36) were transferred to fresh Krebs-Henseleit saline containing one of the following concentrations of NE: (a) 1.24 x 10‾⁵ M; (b) 1.24 x 10‾⁶ M; (c) 1.24 x 10‾⁷ M; (d) 5.24 x 10‾⁸ M; (e) 1.24 x 10‾⁸ M; and (f) 1.24 x 10‾⁹ M. In all preparations, treatment with NE resulted in an immediate reduction in fluid production, and reabsorptions were observed at the higher doses. Sodium followed the movement of water in every case. The threshold dose was calculated at 3.16 x 10‾¹⁰ M. Phentolamine blocked the effect of NE, reinforcing the importance of pulmonary alpha-adrenergic receptors in the fetal guinea pig. There was no relationship between age and degree of response with treatment of either epinephrine or NE, but fetuses under 78.0 g did not respond to NE. The effect of ACh was examined. Lungs (n=24) were transferred to fresh Krebs-Henseleit saline containing one of the following concentrations of ACh: (a) 10‾⁴ M; (b) 10‾⁵ M; (c) 10‾⁶ M; and (d) 10‾⁸ M. At the three top doses, immediate and powerful reabsorptions of pulmonary fluid were observed in older fetuses (60 days gestation and above); significant falls were observed in the younger fetuses. This result was unexpected, as it was hypothesized that ACh would stimulate fluid production. The threshold dose for ACh was between 10‾⁶ M and 10‾⁸ M. Phentolamine blocked the effect of ACh. This result suggested that reabsorption is a result of an indirect effect of ACh acting through pulmonary alpha receptors. The results in this study show that epinephrine, NE, AVP and ACh are all important promoters of fetal pulmonary fluid removal in the fetal guinea pig. Pulmonary alpha-adrenergic receptors mediate the effects of epinephrine, NE and ACh (indirectly). The conclusions drawn from this study emphasize the importance of species' comparison in fetal research. LIST OF ABBREVIATIONS AVP Arginine Vasopressin NE Norepinephrine DOPA dihydroxyphenylalanine PNMT Phenylethanolamine n-methyltransferase ACh Acetylcholine
Science, Faculty of
Zoology, Department of
Graduate
APA, Harvard, Vancouver, ISO, and other styles
3

Lee, Pei-Chung. "Effector Secretion Control by the Pseudomonas aeruginosa Type III Secretion System." Case Western Reserve University School of Graduate Studies / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1301596980.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Sani, Musa. "Weapons of mass secretion the type III secretion system of Shigella flexneri /." [S.l. : Groningen : s.n. ; University Library Groningen] [Host], 2007. http://irs.ub.rug.nl/ppn/304674354.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Gabert, Vince Morllen. "Pancreatic secretion in pigs." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/nq21570.pdf.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Burquez-Montijo, Jose Alberto. "Studies on nectar secretion." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235811.

Full text
Abstract:
This thesis explores the dynamic patterns in the secretion, reabsorption and concentration of nectar, and their relation with microclimate, flower visitors and the innervation of the nectaries. Case studies are presented, comprising Impatiens glandulifera, Brassica napus, Fritillaria imperialis and Borago officinalis. Nectar secretion rate and nectar solute concentration are affected by the environment, and probably by the genetic composition of the plants. Significant differences in nectar secretion rate and nectar concentration are found between plants, between times of the day and between days, but not among flowers on the same plant. Correlation matrices and correlograms help us to disentangle multiple interactions. In all the species studied, the environmental factor most likely to affect nectar secretion rate seems to be the temperature of the air. Other factors also contribute to explain the variation in nectar secretion rate, among them the stand age (probably acting through the relative sink strength of the flowers), and the number of flowers and fruits per module. The supply of assimilates to the nectary is explored by experimental defoliations and deprivation of light. In both cases an immediate response is elicited, but the degree of response varies between species. Brassica napus, for example, is much less sensitive to light deprivation or total defoliation than Fritillaria imperialis. Nectar solute concentration seems to depend mainly on the relative humidity of the air. However, some evidence suggests that plant water status might affect nectar concentration. These results, obtained from field experiments, were confirmed in controlled conditions in growth chambers. Other minor factors probably play a role, but their effects are obscured by complex physiological interactions. We conclude that in a given plant the nectar secretion rate will depend mainly on its physiological age, and on variations in air temperature, while the nectar solute concentration at a given moment will be mainly the product of the short-term plant-microclimate interaction. In this context, constraints on the evolution of nectar presentation systems are considered.
APA, Harvard, Vancouver, ISO, and other styles
7

Song, Soon Hoo. "Dynamics of insulin secretion." Thesis, University of Edinburgh, 2000. http://hdl.handle.net/1842/22645.

Full text
Abstract:
I undertook four related projects that address the pattern of insulin secretion and the specific mechanisms by which b-cell positively influences these. Project one. Direct measurement of the pattern of insulin release in the portal vein (immediately downstream of the pancreas) in human subjects in the basal state and in response to glucose stimulation. Project two. Examination of the effects of acute inhibition of insulin secretion in humans with Type 2 diabetes mellitus by diazoxide on the pattern of insulin secretion. Project three. Development and validation of a method to quantify pulsatile insulin release by cultured human islets in an open loop perifusion system. Project four. Use of the system developed and validated in project 3 to test the hypothesis that transient b-cell rest in human islets induced by diazoxide (inhibiting insulin secretion by opening b cell potassium channels) prevents loss of the first phase insulin release and pulsatile insulin secretion caused by culture of the islets in glucose concentrations comparable to those seen in Type 2 diabetes mellitus. In these experiments, the following observations were made; (1) when insulin secretion was measured, in humans directly in the portal circulation, hyperglycaemia enhanced insulin secretion through the specific mechanism of amplification of the secretory burst mass while the insulin pulse frequency remained unchanged. (2) Acute partial inhibition of insulin secretion by diazoxide in patients with Type 2 diabetes mellitus did not effect the regularity of insulin secretion but did decrease the insulin clearance rate. (3) A deconvolution programme was established that was able to detect 90% of insulin pulses delivered by single human islets. (4). Using the novel islet perfusion system it was possible to show that human islets cultured at high glucose concentrations lost first phase insulin secretion and insulin pulse amplitude but these were restored in islets exposed to a transient period of b-cell rest. The overall conclusion of these studies is that the dynamics of insulin secretion are important as well as the absolute secretion rate and that the abnormalities in these dynamics may be related in part to b cell insulin stores that can be manipulated by transient b cell rest.
APA, Harvard, Vancouver, ISO, and other styles
8

Losada, Bohannon Liliana Cristina. "Identification and secretion of effectors from the Pseudomonas syringae type III secretion system." College Park, Md. : University of Maryland, 2004. http://hdl.handle.net/1903/1803.

Full text
Abstract:
Thesis (Ph. D.) -- University of Maryland, College Park, 2004.
Thesis research directed by: Molecular and Cell Biology. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
APA, Harvard, Vancouver, ISO, and other styles
9

Bulahan, Rhobe Justine Artates. "Characterizing potential secretion components that increase secretion of recombinant proteins in Pichia Pastoris." Scholarly Commons, 2012. https://scholarlycommons.pacific.edu/uop_etds/812.

Full text
Abstract:
The methylotropic yeast Pichia pastoris has been used for many applications, particularly for its ability to produce and readily secrete heterologous proteins. Nonetheless, there are obstacles in making this useful yeast into a more efficient secretion system that readily secretes problem proteins. In the Lin-Cereghino lab, mutant strains were developed by the method of restriction enzyme mediated integration. These mutants have the ability to secrete β-galactosidase at higher levels in comparison to the wild type. This study focused on characterizing the specific mutant ah2 for its ability to secrete HRP, SLPI, and CALB lipase proteins, as well as using transmission electron microscopy to observe the effect of the pREMI-Z mutation on the morphology. Analysis of the Ah2 protein resulted in a comparative β-galactosidase secretion study, as well as a growth rate study, between the original pREMI-Z ah2 mutant and ah2 mutant cells that were transformed with pKanB-AH2 rescue construct. Lastly, a cell localization experiment was done to examine where Ah2p localizes. By these analyses, we gain a bit more understanding of the P. pastoris secretion pathway, while also outlining a procedure by which to characterize the other pREMI-Z mutants.
APA, Harvard, Vancouver, ISO, and other styles
10

PEREZ, FRANCK. "Secretion regulee et secretion non conventionnelle : les homeoproteines comme outils et comme objets d'etudes." Paris 6, 1995. http://www.theses.fr/1995PA066693.

Full text
Abstract:
Nous nous sommes d'abord attaches a developper un nouvel outil permettant l'etude, non invasive, de la secretion regulee. Nous avons tire parti des proprietes de l'homeodomaine d'antennapedia (antphd) qui a la capacite de traverser les membranes des cellules en culture par un mecanisme independant de l'endocytose classique. Nous avons fusionne a antphd les 33 acides amines carboxy-terminaux d'une petite proteine g, rab3a, impliquee dans l'exocytose regulee. Nous avons montre que la proteine de fusion ar3ac est internalisee sans degradation par des cellules en culture, validant ainsi antphd comme vecteur de peptides exogenes. Nous avons utilise ce vecteur pour etudier la fonction de rab3 dans le modele des cellules a prolactine. L'internalisation de la sequence carboxy-terminale de rab3a ainsi que celle de rab3b (et non celle de rab1 ou rab2) bloque efficacement l'exocytose regulee de ces cellules. Ceci montre que les proteines rab3 sont impliquees dans le controle de l'exocytose regulee et suggere que, malgre leur divergence, les sequences hypervariables des deux proteines rab3 interagissent avec un meme regulateur specifique. La mise en evidence de l'internalisation d'antphd a suggere un modele ou les proteines a homeodomaine pourraient, en plus de leur fonction en tant que facteurs de transcription, etre des facteurs nucleaires a action paracrine. Nous avons d'abord teste la capacite a traverser les membranes cytoplasmiques. Ajoutee dans le milieu extracellulaire, la proteine hoxa-5 est internalisee et s'accumule dans le noyau des cellules traitees. Les homeoproteines etant depourvues de peptide signal, leur eventuelle secretion ne pourrait se faire que par des voies non conventionnelles. Nos experiences de traduction in vitro et de surexpression dans des fibroblastes suggerent effectivement une interaction entre la proteine hoxa-5 et l'appareil de secretion, laissant ouverte la possibilite d'un role paracrine pour les proteines a homeodomaine
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "Secretion"

1

Salehi, Sebastian Albert. Insulin secretion: Modulation of islet acid glucan-1,4-gas-glucosidase activity by selective inhibitors, Ca2+ and nitric oxide. Lund [Sweden]: Department of Pharmacology, Lund University, 1995.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

Economou, Anastassios, ed. Protein Secretion. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60327-412-8.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

S, Davison J., ed. Gastrointestinal secretion. London: Wright, 1989.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
4

Velibor, Krsmanović, and Whitfield James F, eds. Malignant cell secretion. Boca Raton: CRC Press, 1990.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

Pompa, Andrea, and Francesca De Marchis, eds. Unconventional Protein Secretion. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3804-9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Jiang, Liwen, ed. Plant Protein Secretion. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7262-3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Jena, Bhanu P. NanoCellBiology of Secretion. Boston, MA: Springer US, 2012. http://dx.doi.org/10.1007/978-1-4614-2438-3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Journet, Laure, and Eric Cascales, eds. Bacterial Secretion Systems. New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3445-5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

T, Mason W., Sattelle David B, and Company of Biologists, eds. The Secretory event: Based on a discussion meeting arranged by the Company of Biologists Limited at Kolimbari, Crete, in March 1988. Cambridge: Company of Biologists, 1988.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

Society of General Physiologists. (42nd 1988 Marine Biological Laboratory). Secretion and its control: Society of General Physiologists, Biological Laboratory, Woods Hole, Mass., 1 - 10 Stpt., 1988. New York: Rockefeller University Press, 1988.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "Secretion"

1

Rothman, Stephen. "Secretion." In Proteins Crossing Membranes, 47–50. Boca Raton : Taylor & Francis, 2019.: CRC Press, 2019. http://dx.doi.org/10.1201/9780429020810-7.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Ruan, Zhi, and Tsuneya Ikezu. "Tau Secretion." In Advances in Experimental Medicine and Biology, 123–34. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-32-9358-8_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Calam, John. "Acid secretion." In Helicobacter pylori Infection, 53–61. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-2216-0_6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Cheli, Rodolfo, Alessandro Perasso, and Attilio Giacosa. "Gastric Secretion." In Gastritis, 144–58. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-71845-8_9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Owyang, Chung, and John A. Williams. "Pancreatic Secretion." In Yamada' s Textbook of Gastroenterology, 450–73. Oxford, UK: John Wiley & Sons, Ltd, 2015. http://dx.doi.org/10.1002/9781118512074.ch25.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Allen, Adrian, Andrew C. Hunter, and Anwar H. Mall. "Mucus Secretion." In Gastric Cytoprotection, 75–90. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4684-5697-4_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Jönsson, Ann-Cathrine, and Susanne Holmgren. "Gut secretion." In The Comparative Physiology of Regulatory Peptides, 256–71. Dordrecht: Springer Netherlands, 1989. http://dx.doi.org/10.1007/978-94-009-0835-2_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Roshchina, Victoria V., and Valentina D. Roshchina. "Intratissular Secretion." In The Excretory Function of Higher Plants, 25–66. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-78130-8_3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Roshchina, Victoria V., and Valentina D. Roshchina. "External Secretion." In The Excretory Function of Higher Plants, 67–130. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-78130-8_4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Flaumenhaft, Robert. "Platelet Secretion." In Platelets in Thrombotic and Non-Thrombotic Disorders, 353–66. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-47462-5_26.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Secretion"

1

Koike, Kazuo, and Holm Holmsen. "REQUIREMENT FOR THROMBIN RECEPTOR OCCUPANY DURING PLATELET SECRETION UNDER AGGREGATING CONDITIONS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644469.

Full text
Abstract:
We have previously showed that hirudin abruptly arrests thrombin-induced secretion of acid hydrolase at any stage of its progress, whereas it only affects dense granule secretion only at its initial stages; these results have been interpreted to show that acid hydrolase secretion requires sustained while dense granule secreion ony requires a brief receptor occupancy (Holmsen et al. JBC 256(1981)9393). The requirement for receptor occupancy in thrombin-induced α-granule secretion and secretion during aggregation have been studied. Increasing concentrations of thrombin were added to gel-fitered platelets containing a constant, high concentration of hirudin. Dense granule secretion was initiated at lower thrombin concentration than those required for α-granule secretion and aggregation; acid hydrolase secretion required higher concentrations. A 14-fold exess of hirudin produced abrupt stop of dense granule secretion and α-granule secretion when added to platelets shortly after thrombin; it had no affect after these secretory process had reached 30% of their maximal values. Acid hydrolase secretion was, however, abruptly stopped by hirudin at any stage. When the platelets were allowed to aggregate, all three secretory processes increased their rates and could now be abruptly stopped by hirudin at any stage. Aggregation (optical) occurred slower than dense granule andoαgranule secretion, and was reversed by hirudin when added before it had reached 30% of its maximum. It is concluded thatαgranule secretion, like dense granule secretion, only requires a short receptor occupancy to be completed, in contrast to the requirement for sustained occupancy for hydrolase secretion.α-granule secretion might, however, require longer occupancy than dense granule secretion. It is possible that aggregation potentiates all secretory responses through close cell contact and that the abrupt inhibition by hirudin of all secretions may have been caused by its effect on the slower aggregation.
APA, Harvard, Vancouver, ISO, and other styles
2

Tsomartova, Dibakhan, Nataliya Yaglova, Sergey Obernikhin, Svetlana Nazimova, and Valentin Vasilyevich Yaglov. "ALTERED CYTOPHYSIOLOGY OF EPINEPHRINE-PRODUCING CELLS IN RATS AFTER CHRONIC EXPOSURE TO LOW DOSES OF DDT." In NEW TECHNOLOGIES IN MEDICINE, BIOLOGY, PHARMACOLOGY AND ECOLOGY. Institute of information technology, 2021. http://dx.doi.org/10.47501/978-5-6044060-1-4.12.

Full text
Abstract:
Chronic low-dose exposure to dichlorodiphenyltrichloroethane does not diminish epinephrine production since epinephrine-secreting adrenal cells significantly intensify mitochondrial ac-tivity to restore epinephrine secretion.
APA, Harvard, Vancouver, ISO, and other styles
3

Takahashi, Nozomi, Hiromi Nakamura, Takuji Narumi, Michitaka Hirose, and Kazuma Aoyama. "Electrical Stimulation Promotes Saliva Secretion: Proposition of Novel Interaction via Saliva Secretion." In CHI '20: CHI Conference on Human Factors in Computing Systems. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3334480.3382952.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Gomes Vieira Carvalho, Thainá, Joyce Mothé de Souza, Elisa Haddad Pessanha Rangel, Caio Gomes Muniz, Julia Maria Maia de Azevedo, and Luciano Matos Chicayban. "Bronchial hygiene technique in patients with cystic fibrosis." In 7th International Congress on Scientific Knowledge. Biológicas & Saúde, 2021. http://dx.doi.org/10.25242/8868113820212402.

Full text
Abstract:
Cystic Fibrosis is characterized by excess pulmonary secretions that cause recurrent respiratory infections, with consequent deterioration of gas exchange. Bronchial hygiene techniques aim to mobilize secretions from the peripheral airways so that they can be eliminated by coughing or tracheal aspiration. To identify the effects of different bronchial hygiene techniques on improving lung function in patients with Cystic Fibrosis. Through a systematic review of the literature, randomized controlled trials (RCTs) published between 2007 and 2021 were selected, according to the highest score in the PEDro score. The search involved the PEDro and PubMed databases, using the following keywords: bronchial hygiene. Six ECR`s were included. One study performedthe techniques during anesthesia and observed increased resistance and reduced compliance. Regarding FEV1, 3 RCTs with hospitalized patients showed improvement in lung function, regardless of the technique used. In outpatients, there was no improvement. Regarding secretion weight, the cough machine produced more secretion than autogenous drainage, as well as a drop in saturation after the 2-min walk test, and increased FEV1. Bronchial hygiene techniques in patients with cystic fibrosis have been shown to be effective in removing mucus, with consequent improvement in lung function and aerobic fitness.
APA, Harvard, Vancouver, ISO, and other styles
5

Moffat, E. H., R. H. Furlong, A. L. Bloom, and J. C. Giddings. "A MURINE MODEL FOR FACTOR VIII ANTIBODY ANTI-IDIOTYPE REAGENTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644030.

Full text
Abstract:
The regulation of factor VIII antibody (FVIIIAb) production in haemophilic and non-haemophilic patients may be effected by anti-idiotype (Aid) antibodies which specifically react with FVIIIAb. Aid antibodies (reagents) were prepared from rabbits immunised with murine monoclonal FVIIIAb. Immuno fluorescent microscopy and cell culture studies were performed using murine hybridoma cells which secreted the FVIIIAbs.Immuno fluorescence studies examined the ability of the Aid reagents to bind to acetone fixed FVIIIAb secreting hybridoma cells. Positive surface membrane and intra-cytoplasmic staining patterns were seen with the Aid reagent when incubated with the corresponding murine hybridoma cell line. This reaction was blocked subsequent to the addition of the corresponding monoclonal FVIIIAb but was preserved when unrelated monoclonal FVIIIAb was incubated with the hybridoma cells. No fluorescence was observed when Aid reagent was incubated with unrelated FVIIIAb secreting hybridoma cultures.Following the addition of Aid reagent to FVIIIAb secreting hybridoma cultures and incubation for 19 hours, the resultant hybridoma supernatants were examined for FVIIIAb content using an immunoradiometric technique. The Aid reagent failed to inhibit FVIIIAb secretion by hybridoma cells. Thus, although Aid reagents were capable of binding to fixed FVIIIAb secreting cells, they failed to inhibit FVIIIAb secretion from hybridoma cultures. The conjugation of Aid reagent with immunotoxin may however have cytotoxic potential. The murine model provides a method for the study of Aid regulation of FVIIIAb production in haemophilia.
APA, Harvard, Vancouver, ISO, and other styles
6

Bowen-Pope, D. F., C. Gajdusek, J. Harlan, P. Nawroth, R. Ross, K. S. Sakariassen, and D. Stern. "REGULATION OF GROWTH FACTOR PRODUCTION BY ENDOTHELIAL CELLS IN RESPONSE TO COAGULATION AND INFLAMATORY FACTORS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642947.

Full text
Abstract:
Platelet-derived growth factor (PDGF) is a polypeptide growth factor first discovered in, and purified from, human blood platelets. As assayed by its ability to stimulate proliferation of cultured vascular smooth muscle cells, PDGF is the major mitogen in human whole blood serum. PDGF has also been reported to be chemotactic for fibroblasts, vascular smooth muscle cells, and leukocytes, and to be able to stimulate contraction of arterial smooth muscle. This, spectrum of activities suggests that PDGF could play a significant role in several vascular processes, including wound repair and the formation of atherosclerotic lesions (reviewed in Ross et al., 1986 Cell 46:155). Several cell types in addition to the platelet have now been shown to be capable of secreting PDGF-like molecules. In culture, vascular endothelial cells from many sources secrete significant levels of PDGF (DiCorleto and Bowen-Pope, 1983 PNAS 80:1919). Rates of secretion can be increased four fold and more bythe activated procoagulants thrombin (Harlan et al 1986 J. Cell Biol. 103:1129) and factor Xa (Gajdusek et al 1986 J. Cell Biol. 103:419). Thrombin stimulates secretion by the earliest times measurable (about 1.5hr) and this early response is not diminished by inhibitors of protein and RNA synthesis. Nevertheless, unlike secretion from the platelet, stimulated secretion does not represent release of sequestered active PDGF since no reservoir of active PDGF can be detected within the cells prior to stimulation. It is likely therefore that stimulation of secrtion involves the activation or unmasking of an inactive form of PDGF. The proteolytic activities of thrombin and Xa are necessary for activation of secretion but the mechanism does not seem to to involve direct proteolytic activation by thrombin of a precursor since thrombin treatment does not generate active PDGF in freeze-thawed preparations of endothelial cells. We have recently found that tumor necrosis factor alpha (TNF) and gamma interferon (IFN) can stimulate increased rates of secretion of PDGF by cultured human saphenous vein and umbilical vein endothelial cells. Stimulation by a combination of the two is more than additive. In contrast to the rapid kinetics of stimulation by thrombin and Xa, TNF and IFN do not measurably increase secretion for at lease four hrs. This delayed kinetics is paralleled by increases in mRNA encoding the two subunit chains of PDGF ("A" and "B") and it seems likely that in this case stimulation of secretion results from increased rates of mRNA and protein synthesis. Since evidence is accumulating that TNF and IFN are both present in human atherosclerotic lesions, it is possible that they help stimulate production of endothelial cell-derived mitogens, including PDGF and thus contribute to the development of the lesion.
APA, Harvard, Vancouver, ISO, and other styles
7

Flores Gonzalez, J. R., A. Kushwaha, C. Kernell, M. Bleckhman, O. N. Hoang, A. M. Jaramillo, M. J. Tuvim, and B. F. Dickey. "Syntaxin 3 Mediates Baseline Mucin Secretion." In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a2158.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Lai, Kecheng, Yuqi Liu, Qikun He, Ming Yi, and Tsutomu Fujinami. "Saliva Secretion as Indicator of Appetite." In ICMHI 2021: 2021 5th International Conference on Medical and Health Informatics. New York, NY, USA: ACM, 2021. http://dx.doi.org/10.1145/3472813.3473198.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Athayde, C. M., and M. C. Scrutton. "ROLE OF GUANINE NUCLEOTIDES IN Ca2+ - DEPENDENT LYSOSOMAL SECRETION FROM ELECTROPERMEABILISED PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644513.

Full text
Abstract:
Previous studies have shown that the maximal extent of Ca2+ dependent secretion of β-N-acetylglucosaminidase (B-N-AcGlc) from electropermeabilised human platelets can be enhanced by addition of thrombin or of 1-oleyl-2-acetylglycerol or 12-0-tetradecanoyl phorbol-13-acetate without a significant alteration in the EC^q for Ca2+. (Knight et al. Europ. J. Biochem.,143337 (1984)). We have found a similar Ca2+ dependent increase in the maximal extent of β-N-AcGlc and β-galactosidase secretion on addition of metabolically stable analogues of GTP (GTPγS and GppNHp) in the absence of thrombin or of GTP added in the presence of a nonsaturating concentration of thrombin. The EC50 values for GTP and GppNHp do not differ significantly for β-N-AcGlc and 3H-5HT secretion, but GTPγS is significantly more effective for 3H-5HT secretion.The time course of β-N-AcGlc secretion induced by GTPγS shows a significant delay as compared with that induced by thrombin + Ca2+. No significant differences could be detected between the properties of β-N-AcGlc or β-galactosidase secretion in this system. The results are consistent with involvement of a GTP binding protein (Np) in receptor-phospholipase C coupling mediating lysosomal secretion, but provide no indication that an additional protein of this type (Ne) is involved as has been proposed for lysosomal secretion from neutrophils. We have thus far failed to find evidence for heterogeneity in lysosomal secretion in this system (supported by SERC and Ciba-Geigy).
APA, Harvard, Vancouver, ISO, and other styles
10

Fathi, Mohsen, Robiya Joseph, Melisa Martinez-Paniagua, Jay R. Adolacion, Xingyue An, Ankit Mahendra, Konrad Gabrusiewicz, Sujash Chatterjee, Sendurai A. Mani, and Navin Varadarajan. "Abstract 2907: Exosome secretion is an inheritable property of cancer cells: Single-cell profiling of exosome secretion." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-2907.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "Secretion"

1

Flowers, Ann M. Secretion of Heterologous Proteins from Escherichia coli. Fort Belvoir, VA: Defense Technical Information Center, December 2000. http://dx.doi.org/10.21236/ada391190.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Vogel, Christine. Preventing Prostate Cancer Metastasis by Targeting Exosome Secretion. Fort Belvoir, VA: Defense Technical Information Center, October 2014. http://dx.doi.org/10.21236/ada611806.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Starmer, Frank. Models of Excitation-Secretion Coupling in Pituitary Cells. Fort Belvoir, VA: Defense Technical Information Center, June 1991. http://dx.doi.org/10.21236/ada238594.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Liu, Lu. Neural control of facial sweat gland secretion in horses. Ames (Iowa): Iowa State University, January 2019. http://dx.doi.org/10.31274/cc-20240624-1047.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Chuck, Steven L. The Non-Classical Secretion of Thioredoxin from Breast Cancer Cells. Fort Belvoir, VA: Defense Technical Information Center, June 2002. http://dx.doi.org/10.21236/ada407677.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Chuck, Steven L. The Non-Classical Secretion of Thioredoxin from Breast Cancer Cells. Fort Belvoir, VA: Defense Technical Information Center, June 2004. http://dx.doi.org/10.21236/ada426431.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Chuck, Steven L. The Non-Classical Secretion of Thioredoxin from Breast Cancer Cells. Fort Belvoir, VA: Defense Technical Information Center, June 2003. http://dx.doi.org/10.21236/ada425885.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Bartlow, Frederick. Factors affecting thyrotropin secretion in superfused rat anterior pituitary cells. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.3133.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Elbaum, Michael, and Peter J. Christie. Type IV Secretion System of Agrobacterium tumefaciens: Components and Structures. United States Department of Agriculture, March 2013. http://dx.doi.org/10.32747/2013.7699848.bard.

Full text
Abstract:
Objectives: The overall goal of the project was to build an ultrastructural model of the Agrobacterium tumefaciens type IV secretion system (T4SS) based on electron microscopy, genetics, and immunolocalization of its components. There were four original aims: Aim 1: Define the contributions of contact-dependent and -independent plant signals to formation of novel morphological changes at the A. tumefaciens polar membrane. Aim 2: Genetic basis for morphological changes at the A. tumefaciens polar membrane. Aim 3: Immuno-localization of VirB proteins Aim 4: Structural definition of the substrate translocation route. There were no major revisions to the aims, and the work focused on the above questions. Background: Agrobacterium presents a unique example of inter-kingdom gene transfer. The process involves cell to cell transfer of both protein and DNA substrates via a contact-dependent mechanism akin to bacterial conjugation. Transfer is mediated by a T4SS. Intensive study of the Agrobacterium T4SS has made it an archetypal model for the genetics and biochemistry. The channel is assembled from eleven protein components encoded on the B operon in the virulence region of the tumor-inducing plasmid, plus an additional coupling protein, VirD4. During the course of our project two structural studies were published presenting X-ray crystallography and three-dimensional reconstruction from electron microscopy of a core complex of the channel assembled in vitro from homologous proteins of E. coli, representing VirB7, VirB9, and VirB10. Another study was published claiming that the secretion channels in Agrobacterium appear on helical arrays around the membrane perimeter and along the entire length of the bacterium. Helical arrangements in bacterial membranes have since fallen from favor however, and that finding was partially retracted in a second publication. Overall, the localization of the T4SS within the bacterial membranes remains enigmatic in the literature, and we believe that our results from this project make a significant advance. Summary of achievements : We found that polar inflations and other membrane disturbances relate to the activation conditions rather than to virulence protein expression. Activation requires low pH and nutrient-poor medium. These stress conditions are also reflected in DNA condensation to varying degrees. Nonetheless, they must be considered in modeling the T4SS as they represent the relevant conditions for its expression and activity. We identified the T4SS core component VirB7 at native expression levels using state of the art super-resolution light microscopy. This marker of the secretion system was found almost exclusively at the cell poles, and typically one pole. Immuno-electron microscopy identified the protein at the inner membrane, rather than at bridges across the inner and outer membranes. This suggests a rare or transient assembly of the secretion-competent channel, or alternatively a two-step secretion involving an intermediate step in the periplasmic space. We followed the expression of the major secreted effector, VirE2. This is a single-stranded DNA binding protein that forms a capsid around the transferred oligonucleotide, adapting the bacterial conjugation to the eukaryotic host. We found that over-expressed VirE2 forms filamentous complexes in the bacterial cytoplasm that could be observed both by conventional fluorescence microscopy and by correlative electron cryo-tomography. Using a non-retentive mutant we observed secretion of VirE2 from bacterial poles. We labeled the secreted substrates in vivo in order detect their secretion and appearance in the plant cells. However the low transfer efficiency and significant background signal have so far hampered this approach.
APA, Harvard, Vancouver, ISO, and other styles
10

Dashek, W. V. A study of overproduction and enhanced secretion of enzymes. Quarterly report. Office of Scientific and Technical Information (OSTI), September 1993. http://dx.doi.org/10.2172/376395.

Full text
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Secretion' for particular source types:
Journal articles Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography