Dissertations / Theses on the topic 'Schrenk'
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Volmer, Rebekka [Verfasser], Christine [Akademischer Betreuer] Hertler, Friedemann [Akademischer Betreuer] Schrenk, and Bruno [Akademischer Betreuer] Streit. "Struktur und gemeinsame Nutzung von Nahrungsressourcen in fossilen Carnivoren-Gilden / Rebekka Volmer. Gutachter: Friedemann Schrenk ; Bruno Streit. Betreuer: Christine Hertler ; Friedemann Schrenk." Frankfurt am Main : Univ.-Bibliothek Frankfurt am Main, 2013. http://d-nb.info/1044276339/34.
Full textSchikora, Tim F. [Verfasser], Friedemann [Akademischer Betreuer] Schrenk, and Axel [Akademischer Betreuer] Janke. "Climate-linked temporal and spatial patterns in the evolution of African bovidae / Tim F. Schikora. Gutachter: Friedemann Schrenk ; Axel Janke. Betreuer: Friedemann Schrenk." Frankfurt am Main : Univ.-Bibliothek Frankfurt am Main, 2013. http://d-nb.info/104441328X/34.
Full textSchrenk, Sebastian [Verfasser], and Bernhard [Akademischer Betreuer] Hensel. "Abbauverhalten degradierbarer Magnesiumlegierungen in körperähnlichen Elektrolyten / Sebastian Schrenk. Betreuer: Bernhard Hensel." Erlangen : Universitätsbibliothek der Universität Erlangen-Nürnberg, 2011. http://d-nb.info/1015888887/34.
Full textKirchmair, Ivana [Verfasser], Friedemann Gutachter] Schrenk, and Meike [Gutachter] [Piepenbring. "Biogeography of West African gallery forests / Ivana Kirchmair ; Gutachter: Friedemann Schrenk, Meike Piepenbring." Frankfurt am Main : Universitätsbibliothek Johann Christian Senckenberg, 2017. http://d-nb.info/1141790491/34.
Full textKirchmair, Ivana Verfasser], Friedemann [Gutachter] Schrenk, and Meike [Gutachter] [Piepenbring. "Biogeography of West African gallery forests / Ivana Kirchmair ; Gutachter: Friedemann Schrenk, Meike Piepenbring." Frankfurt am Main : Universitätsbibliothek Johann Christian Senckenberg, 2017. http://d-nb.info/1141790491/34.
Full textZubairov, Bulat. "Application of dendroclimatic methods in assessment of climate change impacts on the annual growth of Schrenk spruce in the Ile River basin, southeastern Kazakhstan." Doctoral thesis, Humboldt-Universität zu Berlin, 2020. http://dx.doi.org/10.18452/21042.
Full textForests are very important for people’s livelihood. They provide important ecosystem services and goods. However there are many threats that interfere with the normal functioning and development of forest ecosystems. The influence of climate change processes on functioning and dynamics of forests in Kazakhstan is understudied, and is observed in the relatively small number of dendroclimatic studies. Additional limitations in understanding of climate-growth relationships arise from insufficient spatial and temporal coverage of those studies and the absence of a qualitative analysis of already available information for identification of common patterns and differences in the impacts of tree growth on climate. The main research goal of this thesis was accordingly to address these limitations. An analysis of all available publications on dendroclimatic studies in Kazakhstan was performed. This analysis not only summarized the available information on climate-growth relationships, but also provided certain information about existed research gaps. Some of those gaps were addressed by an investigation of variation of climate-growth relationships of Schrenk spruce in the Ile River basin. The study revealed certain differences in reaction of earlywood, latewood and total ring width on climate in four mountain ranges of the Ile River basin. The strong influence of climate change was observed in a decreasing temperature signal around the 1970s. Revealed periodicities in the fluctuation of tree-ring indices suggested an influence of certain climate indices such as North Atlantic Oscillation and the Tropospheric Biennial Oscillations. Collectively, the analysis in this dissertation contributes to development of the tree-ting network of Kazakhstan. Obtained information should help in assessment of possible impacts of climate change on Schrenk spruce forests in Kazakhstan.
Som, Rita. "Studies on the resistance of tea plants against Glomerella Cingulata (Stoneman) Spauld & ·Schrenk with Special Reference to the Involvement of Defense Enzymes." Thesis, University of North Bengal, 2008. http://hdl.handle.net/123456789/908.
Full textLüdecke, Tina [Verfasser], Andreas [Gutachter] Mulch, and Friedemann [Gutachter] Schrenk. "Stable isotope-based paleoenvironmental reconstructions of Neogene terrestrial archives / Tina Lüdecke. Gutachter: Andreas Mulch ; Friedemann Schrenk." Frankfurt am Main : Universitätsbibliothek Johann Christian Senckenberg, 2016. http://d-nb.info/1112601236/34.
Full textStegmüller, Simone [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Bildung von DNA‐Addukten durch α‐ und β‑Asaron in primären Rattenhepatozyten / Simone Stegmüller ; Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2020. http://d-nb.info/1221129430/34.
Full textHausinger, Katharina [Verfasser], Dieter [Akademischer Betreuer] Schrenk, and Heike [Akademischer Betreuer] Raddatz. "Vollautomatisch-optische Traubensortierung im Fokus der Qualitätssicherung, -steigerung und -sicherheit / Katharina Hausinger. Betreuer: Dieter Schrenk ; Heike Raddatz." Kaiserslautern : Technische Universität Kaiserslautern, 2014. http://d-nb.info/1047096897/34.
Full textDenzau, Susanne [Verfasser], Wolfgang [Akademischer Betreuer] Wiltschko, and Friedemann [Akademischer Betreuer] Schrenk. "Verhaltensbiologische und histologische Untersuchungen zur Magnetrezeption bei Hühnerküken (Gallus gallus) / Susanne Denzau. Gutachter: Wolfgang Wiltschko ; Friedemann Schrenk." Frankfurt am Main : Univ.-Bibliothek Frankfurt am Main, 2013. http://d-nb.info/1051672635/34.
Full textSchulte-Hubbert, Ruth [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Estragol: Bestimmung von DNA-Addukten in primären Rattenhepatozyten und genotoxisches Potential / Ruth Schulte-Hubbert ; Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2021. http://d-nb.info/1236897374/34.
Full textRaith, Andreas Verfasser], Armin [Akademischer Betreuer] [Gutachter] Lude, and Marcus [Gutachter] [Schrenk. "Das Potential naturnah gestalteter Schulhöfe für informelle Naturerfahrungen / Andreas Raith ; Gutachter: Armin Lude, Marcus Schrenk ; Betreuer: Armin Lude." Ludwigsburg : Pädagogische Hochschule Ludwigsburg, 2017. http://d-nb.info/1134866275/34.
Full textHemgesberg, Melanie Nicole [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Bestimmung von Acrylamid bzw. Glycidamid-DNA-Addukten in Humanblut und tierischen Geweben / Melanie Nicole Hemgesberg ; Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2020. http://d-nb.info/122069133X/34.
Full textMesser, Anastasia Verfasser], and Dieter [Akademischer Betreuer] [Schrenk. "Crosstalk zwischen murinen myeloiden Zellen und fremdstoffmetabolisierenden Hepatomzellen durch Liganden des AhR-Signalweges / Anastasia Messer. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2014. http://d-nb.info/1060371014/34.
Full textCartus, Alexander T. Verfasser], and Dieter [Akademischer Betreuer] [Schrenk. "Metabolismus der Phenylpropene Methyleugenol, (E)-Methylisoeugenol sowie alpha-, beta- und gamma-Asaron / Alexander T. Cartus. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2014. http://d-nb.info/1058104276/34.
Full textBrück, Jens Verfasser], and Dieter [Akademischer Betreuer] [Schrenk. "Einflüsse von niedrig dosiertem Furan auf die Leber in vivo und in vitro / Jens Brück. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2015. http://d-nb.info/1077477953/34.
Full textRaith, Andreas [Verfasser], Armin [Akademischer Betreuer] Lude, and Marcus [Gutachter] Schrenk. "Das Potential naturnah gestalteter Schulhöfe für informelle Naturerfahrungen / Andreas Raith ; Gutachter: Armin Lude, Marcus Schrenk ; Betreuer: Armin Lude." Ludwigsburg : Pädagogische Hochschule Ludwigsburg, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:lg1-opus4-5268.
Full textMesser, Anastasia [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Crosstalk zwischen murinen myeloiden Zellen und fremdstoffmetabolisierenden Hepatomzellen durch Liganden des AhR-Signalweges / Anastasia Messer. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2014. http://d-nb.info/1060371014/34.
Full textBrück, Jens [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Einflüsse von niedrig dosiertem Furan auf die Leber in vivo und in vitro / Jens Brück. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2015. http://d-nb.info/1077477953/34.
Full textRaquet, Nicole [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "In vitro Untersuchungen zum relativen phototoxischen und photogentoxischen Potential natürlich vorkommender Furocumarine und Limettin / Nicole Raquet. Betreuer: Dieter Schrenk." Kaiserslautern : Universitätsbibliothek Kaiserslautern, 2011. http://d-nb.info/1012880931/34.
Full textSeibel, Thomas Michael Verfasser], and Dieter [Akademischer Betreuer] [Schrenk. "Metabolitenmuster im Blut und Genexpressionsmuster von Aryl-Hydrocarbon-Rezeptor-defizienten- und Wildtyp-Mäusen / Thomas Michael Seibel. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2014. http://d-nb.info/1060370956/34.
Full textSeibel, Thomas Michael [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Metabolitenmuster im Blut und Genexpressionsmuster von Aryl-Hydrocarbon-Rezeptor-defizienten- und Wildtyp-Mäusen / Thomas Michael Seibel. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2014. http://d-nb.info/1060370956/34.
Full textMenz, Ulrike Maria [Verfasser], Ottmar [Akademischer Betreuer] Kullmer, and Friedemann [Gutachter] Schrenk. "Evolution und Funktionsvariabilität von bunodonten Molaren bei Primaten / Ulrike Maria Menz ; Gutachter: Ottmar Kullmer, Friedemann Schrenk ; Betreuer: Ottmar Kullmer." Frankfurt am Main : Universitätsbibliothek Johann Christian Senckenberg, 2017. http://d-nb.info/1127135376/34.
Full textMenz, Ulrike Maria Verfasser], Ottmar [Akademischer Betreuer] [Kullmer, and Friedemann [Gutachter] Schrenk. "Evolution und Funktionsvariabilität von bunodonten Molaren bei Primaten / Ulrike Maria Menz ; Gutachter: Ottmar Kullmer, Friedemann Schrenk ; Betreuer: Ottmar Kullmer." Frankfurt am Main : Universitätsbibliothek Johann Christian Senckenberg, 2017. http://d-nb.info/1127135376/34.
Full textUfelmann, Helena [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Toxikologische Relevanz des Vorkommens von Desmethyl-Varianten der Microcystine und des Nodularins in Gewässern und Nahrungsergänzungsmitteln / Helena Ufelmann. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2012. http://d-nb.info/1029293449/34.
Full textBerg, Kerstin [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "In vitro Untersuchungen zur Gentoxizität der Alkenylbenzene α-, β- und γ-Asaron sowie ausgewählter oxidativer Metaboliten / Kerstin Berg. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2016. http://d-nb.info/1103486497/34.
Full textLerp, Hannes [Verfasser], Martin [Akademischer Betreuer] Plath, and Friedemann [Akademischer Betreuer] Schrenk. "The phylogeny of the genus Gazella and the phylogeography and population genetics of arabian species / Hannes Lerp. Gutachter: Martin Plath ; Friedemann Schrenk." Frankfurt am Main : Univ.-Bibliothek Frankfurt am Main, 2014. http://d-nb.info/1049732227/34.
Full textSchrenk, Hannah [Verfasser], zu Castell-Rüdenhausen Wolfgang [Akademischer Betreuer] Graf, Christina [Gutachter] Kuttler, zu Castell-Rüdenhausen Wolfgang [Gutachter] Graf, and Marc-Thorsten [Gutachter] Hütt. "Computing the Adaptive Cycle / Hannah Schrenk ; Gutachter: Christina Kuttler, Wolfgang Graf zu Castell-Rüdenhausen, Marc-Thorsten Hütt ; Betreuer: Wolfgang Graf zu Castell-Rüdenhausen." München : Universitätsbibliothek der TU München, 2021. http://d-nb.info/1233428314/34.
Full textJohann, Esther [Verfasser], Dieter [Akademischer Betreuer] Schrenk, and Stefan Otto [Akademischer Betreuer] Müller. "Improved early in vitro prediction of drug-induced liver injury in man: assessment of novel 3D hepatic models / Esther Johann ; Dieter Schrenk, Stefan Otto Müller." Kaiserslautern : Technische Universität Kaiserslautern, 2018. http://d-nb.info/1170154018/34.
Full textTausch, Jeremy Verfasser], Friedemann [Akademischer Betreuer] Schrenk, and Timothy [Akademischer Betreuer] [Bromage. "A new method for examining hominin dietary strategy: occlusal microwear vector analysis of the Sangiran 7 Homo erectus molars / Jeremy Tausch. Gutachter: Friedemann Schrenk ; Timothy Bromage." Frankfurt am Main : Univ.-Bibliothek Frankfurt am Main, 2012. http://d-nb.info/1044275332/34.
Full textTausch, Jeremy [Verfasser], Friedemann Akademischer Betreuer] Schrenk, and Timothy [Akademischer Betreuer] [Bromage. "A new method for examining hominin dietary strategy: occlusal microwear vector analysis of the Sangiran 7 Homo erectus molars / Jeremy Tausch. Gutachter: Friedemann Schrenk ; Timothy Bromage." Frankfurt am Main : Univ.-Bibliothek Frankfurt am Main, 2012. http://nbn-resolving.de/urn:nbn:de:hebis:30:3-248492.
Full textDas, Gouri. "Studies on the Brown Blight disease of tea Camellia sinensis L. (O.) Kuntze cause by Glomerella cingulata (stoneman) Spauld. & Schrenk. and its interaction with phylloplane microorganisms." Thesis, University of North Bengal, 1995. http://hdl.handle.net/123456789/1088.
Full textNeser, Sylke Verfasser], and Dieter [Akademischer Betreuer] [Schrenk. "Impact of 'Dioxins' on Gene Expression in Mouse Liver in vivo, and in both Rat Liver Cells and Human Blood Cells In Culture / Sylke Neser. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2014. http://d-nb.info/1063933897/34.
Full textLohr, Christiane [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Effects of Polychlorinated Dibenzo-p-Dioxins, Polychlorinated Dibenzofurans, and Polychlorinated Biphenyls in Human Liver Cell Models (in vitro) and in Mice (in vivo) / Christiane Lohr. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2013. http://d-nb.info/1045604054/34.
Full textSchaal, Sonja Verfasser], Armin [Akademischer Betreuer] [Gutachter] Lude, Marcus [Gutachter] [Schrenk, and Christian [Gutachter] Spannagel. "Die Wertschätzung lokaler Biodiversität mit Geogames fördern – die Bedeutung von spielbezogenem Enjoyment im Spiel „FindeVielfalt Simulation“ / Sonja Schaal ; Gutachter: Armin Lude, Marcus Schrenk, Christian Spannagel ; Betreuer: Armin Lude." Ludwigsburg : Pädagogische Hochschule Ludwigsburg, 2017. http://d-nb.info/1134866259/34.
Full textEmanuel, Matthias Stefan [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Formation of a lysine-derived protein conjugate from reaction of (E,E)-muconaldehyde, a potentially hematotoxic metabolite of benzene, with serum albumin / Matthias Stefan Emanuel ; Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2018. http://d-nb.info/1160235503/34.
Full textWolf, Maike [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Rolle der nukleären Rezeptoren CAR und PXR bei der Induktion fremdstoffmetabolisierender Enzyme sowie der Hemmung der UV-induzierten Apoptose durch nicht-dioxinartige PCBs / Maike Wolf ; Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2020. http://d-nb.info/1221129449/34.
Full textNeser, Sylke [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Impact of 'Dioxins' on Gene Expression in Mouse Liver in vivo, and in both Rat Liver Cells and Human Blood Cells In Culture / Sylke Neser. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2014. http://d-nb.info/1063933897/34.
Full textZiege, Madlen [Verfasser], Martin [Gutachter] Plath, and Friedemann [Gutachter] Schrenk. "Effects of gradual anthropogenic habitat alteration on the ecology and behavior of European rabbits (Oryctolagus cuniculus) in and around Frankfurt am Main / Madlen Ziege ; Gutachter: Martin Plath, Friedemann Schrenk." Frankfurt am Main : Universitätsbibliothek Johann Christian Senckenberg, 2017. http://d-nb.info/1143024281/34.
Full textEloy, Ygor Raphael Gomes. "Hole of hydrogen peroxide on the interaction of [Vigna unguiculata (L.) Walp] with the fungus Colletotrichum gloesporioides (Penz.) Penz. & Sacc. [Teleomorpho Glomerella cingulata (Stonem.) Spauld. & Von Schrenk]." Universidade Federal do CearÃ, 2007. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6471.
Full textCowpea is an important legume of the Northeast of Brazil and its yield is affected by Colletotrichum fungi. These fungi are known to occur on and cause disease anthracnose on the broad range of plants. In addition, the rapid and transient accumulation of reactive oxygen species, termed âoxidative burstâ, is one of the earliest observable reaction of plant cells to microbial infection. As plant H2O2 is believed to have direct antimicrobial effects on pathogens it was raised the hypothesis that alteration of H2O2 concentrations by pharmacological compounds could confer differences in susceptibility of the cowpea genotype BR-3-Tracuateua to C. gloeosporioides attack. Thus, seeds were germinated on humid Germitest paper and after four days seedlings were transferred to hydroponic solution. Eight days later, the primary leaves were excised, infiltrated with glucose oxidase (GO+G), salicylic acid (AS), catalase (CAT) and diphenyleneiodonium chloride (DPI) transferred to Petri dishes and inoculated with 2.0 x 105 spores mL-1 fungal suspension on the adaxial surface. After, leaves were placed in darkness and collected at 12, 24, 48 and 72 hours. By GO+G and SA leaf treatments showed 144.96 and 186.05 nmol H2O2 g-1 fresh mass (FM), respectively, and the fungus presented a subcuticular, intramural necrotrophic strategy, forming secondary hyphae associated with a quick spread and a rapid killing of the host cells. On the leaves treated with SA, it was observed lipid peroxidation and the absence of melanized appresoria. However, CAT and DPI treatment leaves presented 55.50 nmol H2O2 g-1 FM and the fungus showed hemibiotrophic infection-type, with globular vesicles and primary and secondary hyphae formation. All results suggest that despite H2O2 influenced directly the fungal infection process-type it does not confer resistance of cowpea to C. gloeosporioides.
FeijÃo-de-corda à um importante legume presente na regiÃo Nordeste do Brasil e sua produÃÃo à afetada por fungos do gÃnero Colletotrichum. Estes fungos sÃo conhecidos por causar antracnose em uma grande variedade de plantas. AlÃm disso, o acÃmulo rÃpido e transiente de espÃcies reativas de oxigÃnio, nomeado de âoxidative burstâ, à uma das primeiras reaÃÃes observÃveis em cÃlulas de plantas diante infecÃÃo microbiana. Como H2O2 produzido por plantas à considerado por apresentar efeitos antimicrobianos diretos, foi levantada a hipÃtese que alteraÃÃes nas concentraÃÃes de H2O2 por substÃncias farmacolÃgicas poderiam conferir diferenÃas na susceptibilidade do genÃtipo de feijÃo-de-corda, BR-3-Tracuateua, ao ataque do fungo C. gloeosporioides. Deste modo, sementes foram germinadas em papÃis Germistest umedecidos e, depois de 4 dias, plÃntulas foram transferidas para soluÃÃo hidropÃnica. Passados oito dias, as folhas primÃrias foram cortadas, infiltradas com glucose oxidase (GO+G), Ãcido salicÃlico (AS), catalase (CAT) e cloreto de difenilenoiodÃnio (DPI), transferidas para placas de Petri e inoculadas com suspensÃo do fungo ajustada para concentraÃÃo de 2,0 x 105 esporos mL-1. Em seguida, as folhas foram mantidas no escuro e foram coletadas em 12, 24, 48 e 72 horas. Folhas tratadas com GO+G e AS mostraram 144,96 e 186,05 nmol H2O2 g-1 de massa fresca (MF), respectivamente, e o fungo apresentou estratÃgia subcuticular, intramural necrotrÃfica, formando hifas secundÃrias associadas com Ãgil crescimento e rÃpida morte das cÃlulas hospedeiras. Nas folhas tratadas com AS, foi observada peroxidaÃÃo de lipÃdios e ausÃncia de apressÃrios melanizados. Contudo, folhas tratadas com CAT e DPI apresentaram 55,50 nmol H2O2 g-1 MF e, o fungo, mostrou modo de infecÃÃo hemibiotrÃfico, com vesÃculas globulares e formaÃÃo de hifas primÃrias e secundÃrias. Todos os resultados sugerem que, apesar do H2O2 tenha influenciado diretamente o modo de infecÃÃo do fungo, este nÃo conferiu resistÃncia no feijÃo-de-corda ao C. gloeosporioides.
Eloy, Ygor Raphael Gomes. "Papel do peróxido de hdrogênio na interação do feijão-de-corda [Vigna unguiculata (L.) Walp] com o fungo Colletotrichum gloesporioides (Penz.) Penz. & Sacc. [Teleomorfo Glomerella cingulata (Stonem.) Spauld. & Von Schrenk]." reponame:Repositório Institucional da UFC, 2007. http://www.repositorio.ufc.br/handle/riufc/18868.
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Cowpea is an important legume of the Northeast of Brazil and its yield is affected by Colletotrichum fungi. These fungi are known to occur on and cause disease anthracnose on the broad range of plants. In addition, the rapid and transient accumulation of reactive oxygen species, termed ‘oxidative burst’, is one of the earliest observable reaction of plant cells to microbial infection. As plant H2O2 is believed to have direct antimicrobial effects on pathogens it was raised the hypothesis that alteration of H2O2 concentrations by pharmacological compounds could confer differences in susceptibility of the cowpea genotype BR-3-Tracuateua to C. gloeosporioides attack. Thus, seeds were germinated on humid Germitest® paper and after four days seedlings were transferred to hydroponic solution. Eight days later, the primary leaves were excised, infiltrated with glucose oxidase (GO+G), salicylic acid (AS), catalase (CAT) and diphenyleneiodonium chloride (DPI) transferred to Petri dishes and inoculated with 2.0 x 105 spores mL-1 fungal suspension on the adaxial surface. After, leaves were placed in darkness and collected at 12, 24, 48 and 72 hours. By GO+G and SA leaf treatments showed 144.96 and 186.05 nmol H2O2 g-1 fresh mass (FM), respectively, and the fungus presented a subcuticular, intramural necrotrophic strategy, forming secondary hyphae associated with a quick spread and a rapid killing of the host cells. On the leaves treated with SA, it was observed lipid peroxidation and the absence of melanized appresoria. However, CAT and DPI treatment leaves presented 55.50 nmol H2O2 g-1 FM and the fungus showed hemibiotrophic infection-type, with globular vesicles and primary and secondary hyphae formation. All results suggest that despite H2O2 influenced directly the fungal infection process-type it does not confer resistance of cowpea to C. gloeosporioides.
Feijão-de-corda é um importante legume presente na região Nordeste do Brasil e sua produção é afetada por fungos do gênero Colletotrichum. Estes fungos são conhecidos por causar antracnose em uma grande variedade de plantas. Além disso, o acúmulo rápido e transiente de espécies reativas de oxigênio, nomeado de ‘oxidative burst’, é uma das primeiras reações observáveis em células de plantas diante infecção microbiana. Como H2O2 produzido por plantas é considerado por apresentar efeitos antimicrobianos diretos, foi levantada a hipótese que alterações nas concentrações de H2O2 por substâncias farmacológicas poderiam conferir diferenças na susceptibilidade do genótipo de feijão-de-corda, BR-3-Tracuateua, ao ataque do fungo C. gloeosporioides. Deste modo, sementes foram germinadas em papéis Germistest® umedecidos e, depois de 4 dias, plântulas foram transferidas para solução hidropônica. Passados oito dias, as folhas primárias foram cortadas, infiltradas com glucose oxidase (GO+G), ácido salicílico (AS), catalase (CAT) e cloreto de difenilenoiodônio (DPI), transferidas para placas de Petri e inoculadas com suspensão do fungo ajustada para concentração de 2,0 x 105 esporos mL-1. Em seguida, as folhas foram mantidas no escuro e foram coletadas em 12, 24, 48 e 72 horas. Folhas tratadas com GO+G e AS mostraram 144,96 e 186,05 nmol H2O2 g-1 de massa fresca (MF), respectivamente, e o fungo apresentou estratégia subcuticular, intramural necrotrófica, formando hifas secundárias associadas com ágil crescimento e rápida morte das células hospedeiras. Nas folhas tratadas com AS, foi observada peroxidação de lipídios e ausência de apressórios melanizados. Contudo, folhas tratadas com CAT e DPI apresentaram 55,50 nmol H2O2 g-1 MF e, o fungo, mostrou modo de infecção hemibiotrófico, com vesículas globulares e formação de hifas primárias e secundárias. Todos os resultados sugerem que, apesar do H2O2 tenha influenciado diretamente o modo de infecção do fungo, este não conferiu resistência no feijão-de-corda ao C. gloeosporioides.
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