Journal articles: 'Schlegelella'

1

Chou, Yi-Ju, Shih-Yi Sheu, Der-Shyan Sheu, Jih-Terng Wang, and Wen-Ming Chen. "Schlegelella aquatica sp. nov., a novel thermophilic bacterium isolated from a hot spring." International Journal of Systematic and Evolutionary Microbiology 56, no. 12 (December 1, 2006): 2793–97. http://dx.doi.org/10.1099/ijs.0.64446-0.

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A moderately thermophilic bacterial strain designated wcf1T, isolated from a hot spring located in the Tainan area, southern Taiwan, was characterized using a polyphasic approach. The cells were Gram-negative, non-pigmented, rod-shaped, non-spore-forming and motile. Phylogenetic analysis using 16S rRNA gene sequences showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus Schlegelella; its only close neighbour was the type strain of Schlegelella thermodepolymerans, K14T (97.8 %). The isolate was clearly distinguishable from other strains using phylogenetic analysis, DNA–DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristics. It was evident from the genotypic and phenotypic data that strain wcf1T represents a novel species in the genus Schlegelella, for which the name Schlegelella aquatica sp. nov. is proposed, with the type strain wcf1T (=BCRC 17557T=LMG 23380T).

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Lütke-Eversloh, Tina, Khaled Elbanna, Margo C. Cnockaert, Joris Mergaert, Jean Swings, Célia M. Manaia, and Alexander Steinbüchel. "Caenibacterium thermophilum is a later synonym of Schlegelella thermodepolymerans." International Journal of Systematic and Evolutionary Microbiology 54, no. 6 (November 1, 2004): 1933–35. http://dx.doi.org/10.1099/ijs.0.63204-0.

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Tang, Biao, Yucong Yu, Junheng Liang, Youming Zhang, Xiaoying Bian, Xiaoyang Zhi, and Xiaoming Ding. "Reclassification of 'Polyangium brachysporum' DSM 7029 as Schlegelella brevitalea sp. nov." International Journal of Systematic and Evolutionary Microbiology 69, no. 9 (September 1, 2019): 2877–83. http://dx.doi.org/10.1099/ijsem.0.003571.

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Medina-Pascual, María J., Sara Monzón, Pilar Villalón, Isabel Cuesta, Fernando González-Romo, and Sylvia Valdezate. "Saezia sanguinis gen. nov., sp. nov., a Betaproteobacteria member of order Burkholderiales, isolated from human blood." International Journal of Systematic and Evolutionary Microbiology 70, no. 3 (March 1, 2020): 2016–25. http://dx.doi.org/10.1099/ijsem.0.004010.

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The taxonomic position of an unknown bacterial strain designated CNM695-12, isolated from the blood of an immunocompromised subject, was investigated via phenotypic, chemotaxonomic, genotypic and genomic analyses. Bacterial cells were determined to be Gram-stain-negative bacilli, aerobic, non-motile and non-spore-forming. The strain showed catalase activity but no oxidase activity. Optimal growth occurred at 37 °C, pH 7 and with 0–1 % NaCl. C16 : 0, summed feature 8 (comprising C18 : 1ω7c /C18:1 ω6c), and C18 : 1ω9c were the most abundant fatty acids, and ubiquinone 8 was the major respiratory quinone. The polar lipids present included phosphatidylglycerol, phosphatidylethanolamine and other aminophospholipids. The 16S rRNA gene sequence showed approximately 93.5 % similarity to those of different species with validly published names within the order Burkholderiales (e.g. Leptothrix mobilis Feox-1T, Aquabacterium commune B8T , Aquabacterium citratiphilum B4T and Schlegelella thermodepolymerans K14T). Phylogenetic analyses based on 16S rRNA gene sequences and concatenated alignments including the sequences for 107 essential proteins, revealed the strain to form a novel lineage close to members of the family Comamonadaceae . The highest average nucleotide identity and average amino acid identity values were obtained with Schlegelella thermodepolymerans K14T (69.6 and 55.7 % respectively). The genome, with a size of 3.35 Mb, had a DNA G+C content of 52.4 mol% and encoded 3056 predicted genes, 3 rRNA, 1 transfer–messengerRNA and 51 tRNA. Strain CNM695-12 thus represents a novel species belonging to a novel genus within the order Burkholderiales , for which the name Saezia sanguinis gen. nov., sp. nov. is proposed. The type strain is CNM695-12T (=DSM 104959T=CECT 9208T).

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Chaudhary, Dhiraj Kumar, Hyosun Lee, Ram Hari Dahal, and Dong-Uk Kim. "Schlegelella koreensis sp. nov., isolated from evaporator core of automobile air conditioning system." Archives of Microbiology 203, no. 5 (March 4, 2021): 2373–78. http://dx.doi.org/10.1007/s00203-021-02206-9.

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Dinwiddie, Darrell, Ashlee K. Bradley, Jesse L. Denson, Joshua L. Kennedy, Walter N. Dehority, Kurt C. Schwalm, and Stephen A. Young. "2356 The nasopharyngeal microbiome is perturbed and associated with increased clinical severity during acute respiratory viral infection." Journal of Clinical and Translational Science 2, S1 (June 2018): 32. http://dx.doi.org/10.1017/cts.2018.137.

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OBJECTIVES/SPECIFIC AIMS: We sought to investigate the role of the host microbiome during severe, acute respiratory infection (ARI) to understand the drivers of both acute clinical pathogenesis. METHODS/STUDY POPULATION: Nasopharyngeal swabs comprised of mixed cell populations at the active site of infection were collected from 192 hospitalized pediatric patients with ARI. We combined comprehensive respiratory virus detection and virus genome sequencing with 16S rRNA gene sequencing to evaluate the microbial content of the airway during ARI. This data was coupled with 11 clinical parameters, which were compiled to create a clinical severity score. The microbiome profiles were assessed to determine if clinical severity of infection, and/or specific virus was associated with increased clinical severity. RESULTS/ANTICIPATED RESULTS: We identified 8 major microbiome profiles classified by dominant bacterial genus, Moraxella, Corynebacterium, Staphylococcus, Haemophilus, Streptococcus, Alloiococcus, Schlegelella, and Diverse. Increased clinical severity was significantly associated with microbiome profiles dominated by Haemophilus, Streptococcus, and Schlegelella, whereas Corynebacterium and Alloiococcus were more prevalent in children with less severe disease. Independent of the microbial community, more than 60% of patients with the highest clinical severity were infected with either respiratory syncytial virus or rhinovirus. DISCUSSION/SIGNIFICANCE OF IMPACT: Our results indicate that individually and in combination, both virus and microbial composition may drive clinical severity during acute respiratory viral infections. It is still unclear how the complex interplay between virus, bacterial community, and the host response influence long-term respiratory impacts, such as the development of asthma. Nonetheless, during ARIs therapeutic interventions such as antibiotics and probiotics may be warranted in a subset of patients that are identified to have both a virus and microbiome profile that is associated with increased pathogenesis to limit both acute and long-term phenotypes.

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Yu, Yucong, Huimin Wang, Biao Tang, Junheng Liang, Lin Zhang, Hongkuan Wang, Xiaoying Bian, et al. "Reassembly of the Biosynthetic Gene Cluster Enables High Epothilone Yield in Engineered Schlegelella brevitalea." ACS Synthetic Biology 9, no. 8 (June 30, 2020): 2009–22. http://dx.doi.org/10.1021/acssynbio.0c00100.

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Kourilova, Xenie, Iva Pernicova, Karel Sedlar, Jana Musilova, Petr Sedlacek, Michal Kalina, Martin Koller, and Stanislav Obruca. "Production of polyhydroxyalkanoates (PHA) by a thermophilic strain of Schlegelella thermodepolymerans from xylose rich substrates." Bioresource Technology 315 (November 2020): 123885. http://dx.doi.org/10.1016/j.biortech.2020.123885.

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9

Elbanna, Khaled, Tina Lütke-Eversloh, Stefanie Van Trappen, Joris Mergaert, Jean Swings, and Alexander Steinbüchel. "Schlegelella thermodepolymerans gen. nov., sp. nov., a novel thermophilic bacterium that degrades poly(3-hydroxybutyrate-co-3-mercaptopropionate)." International Journal of Systematic and Evolutionary Microbiology 53, no. 4 (July 1, 2003): 1165–68. http://dx.doi.org/10.1099/ijs.0.02562-0.

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10

Green, David H., Virginia Echavarri-Bravo, Debra Brennan, and Mark C. Hart. "Bacterial Diversity Associated with the Coccolithophorid AlgaeEmiliania huxleyiandCoccolithus pelagicusf.braarudii." BioMed Research International 2015 (2015): 1–15. http://dx.doi.org/10.1155/2015/194540.

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Coccolithophores are unicellular calcifying marine phytoplankton that can form large and conspicuous blooms in the oceans and make significant contributions to oceanic carbon cycling and atmospheric CO2regulation. Despite their importance, the bacterial diversity associated with these algae has not been explored for ecological or biotechnological reasons. Bacterial membership ofEmiliania huxleyiandCoccolithus pelagicusf.braarudiicultures was assessed using cultivation and cultivation-independent methods. The communities were species rich compared to other phytoplankton cultures. Community analysis identified specific taxa which cooccur in all cultures (MarinobacterandMarivita). Hydrocarbon-degrading bacteria were found in all cultures. The presence of Acidobacteria, Acidimicrobidae,Schlegelella, andThermomonaswas unprecedented but were potentially explained by calcification associated with coccolith production. One strain of Acidobacteria was cultivated and is closely related to a marine Acidobacteria isolated from a sponge. From this assessment of the bacterial diversity of coccolithophores, a number of biotechnological opportunities are evident, from bioprospecting for novel taxa such as Acidobacteria to helping understand the relationship between obligate hydrocarbonoclastic bacteria occurrence with phytoplankton and to revealing bacterial taxa that have a specific association with algae and may be suitable candidates as a means to improve the efficiency of mass algal cultivation.

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Disnard, Julie, Carole Beaulieu, and Richard Villemur. "Composition of the bacterial biota in slime developed in two machines at a Canadian paper mill." Canadian Journal of Microbiology 57, no. 2 (February 2011): 91–104. http://dx.doi.org/10.1139/w10-109.

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During the process of papermaking by pulp and paper plants, a thick and viscous deposits, termed slime, is quickly formed around the paper machines, which can affect the papermaking process. In this study, we explored the composition of the bacterial biota in slime that developed on shower pipes from 2 machines at a Canadian paper mill. Firstly, the composition was assessed for 12 months by DNA profiling with polymerase chain reaction coupled with denaturing gradient gel electrophoresis. Except for short periods (2–3 months), clustered analyses showed that the bacterial composition of the slime varied substantially over the year, with less than 50% similarity between the denaturing gradient gel electrophoresis profiles. Secondly, the screening of 16S rRNA gene libraries derived from 2 slime samples showed that the most abundant bacteria were related to 6 lineages, including Chloroflexi, candidate division OP10, Clostridiales, Bacillales, Burkholderiales, and the genus Deinococcus . Finally, the proportion of 8 bacterial lineages, such as Deinococcus sp., Meiothermus sp., and Chloroflexi, was determined by the Catalyzed Reporter Deposition – Fluorescence In Situ Hybridization in 2 slime samples. The results showed a high proportion of Chloroflexi, Tepidimonas spp., and Schlegelella spp. in the slime samples.

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12

Kourilova, Xenie, Ivana Novackova, Martin Koller, and Stanislav Obruca. "Evaluation of mesophilic Burkholderia sacchari, thermophilic Schlegelella thermodepolymerans and halophilic Halomonas halophila for polyhydroxyalkanoates production on model media mimicking lignocellulose hydrolysates." Bioresource Technology 325 (April 2021): 124704. http://dx.doi.org/10.1016/j.biortech.2021.124704.

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13

Musilova, Jana, Xenie Kourilova, Matej Bezdicek, Martina Lengerova, Stanislav Obruca, Helena Skutkova, and Karel Sedlar. "First Complete Genome of the Thermophilic Polyhydroxyalkanoates-Producing Bacterium Schlegelella thermodepolymerans DSM 15344." Genome Biology and Evolution 13, no. 4 (January 11, 2021). http://dx.doi.org/10.1093/gbe/evab007.

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Abstract Schlegelella thermodepolymerans is a moderately thermophilic bacterium capable of producing polyhydroxyalkanoates—biodegradable polymers representing an alternative to conventional plastics. Here, we present the first complete genome of the type strain S. thermodepolymerans DSM 15344 that was assembled by hybrid approach using both long (Oxford Nanopore) and short (Illumina) reads. The genome consists of a single 3,858,501-bp-long circular chromosome with GC content of 70.3%. Genome annotation identified 3,650 genes in total, whereas 3,598 open reading frames belonged to protein-coding genes. Functional annotation of the genome and division of genes into clusters of orthologous groups revealed a relatively high number of 1,013 genes with unknown function or unknown clusters of orthologous groups, which reflects the fact that only a little is known about thermophilic polyhydroxyalkanoates-producing bacteria on a genome level. On the other hand, 270 genes involved in energy conversion and production were detected. This group covers genes involved in catabolic processes, which suggests capability of S. thermodepolymerans DSM 15344 to utilize and biotechnologically convert various substrates such as lignocellulose-based saccharides, glycerol, or lipids. Based on the knowledge of its genome, it can be stated that S. thermodepolymerans DSM 15344 is a very interesting, metabolically versatile bacterium with great biotechnological potential.

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14

Liang, Junheng, Huimin Wang, Xiaoying Bian, Youming Zhang, Guoping Zhao, and Xiaoming Ding. "Heterologous redox partners supporting the efficient catalysis of epothilone B biosynthesis by EpoK in Schlegelella brevitalea." Microbial Cell Factories 19, no. 1 (September 15, 2020). http://dx.doi.org/10.1186/s12934-020-01439-5.

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Abstract Background Epothilone B is a natural product that stabilizes microtubules, similar to paclitaxel (Taxol); therefore, epothilone B and several derivatives have shown obvious antitumour activities. Some of these products are in clinical trials, and one (ixabepilone, BMS) is already on the market, having been approved by the FDA in 2007. The terminal step in epothilone B biosynthesis is catalysed by the cytochrome P450 enzyme EpoK (CYP167A1), which catalyses the epoxidation of the C12–C13 double bond (in epothilone C and D) to form epothilone A and B, respectively. Although redox partners from different sources support the catalytic activity of EpoK in vitro, the conversion rates are low, and these redox partners are not applied to produce epothilone B in heterologous hosts. Results Schlegelella brevitalea DSM 7029 contains electron transport partners that efficiently support the catalytic activity of EpoK. We screened and identified one ferredoxin, Fdx_0135, by overexpressing putative ferredoxin genes in vivo and identified two ferredoxin reductases, FdR_0130 and FdR_7100, by whole-cell biotransformation of epothilone C to effectively support the catalytic activity of EpoK. In addition, we obtained strain H7029-3, with a high epothilone B yield and found that the proportion of epothilone A + B produced by this strain was 90.93%. Moreover, the whole-cell bioconversion strain 7029-10 was obtained; this strain exhibited an epothilone C conversion rate of 100% in 12 h. Further RT-qPCR experiments were performed to analyse the overexpression levels of the target genes. Gene knock-out experiments showed that the selected ferredoxin (Fdx_0135) and its reductases (FdR_0130 and FdR_7100) might participate in critical physiological processes in DSM 7029. Conclusion Gene overexpression and whole-cell biotransformation were effective methods for identifying the electron transport partners of the P450 enzyme EpoK. In addition, we obtained an epothilone B high-yield strain and developed a robust whole-cell biotransformation system. This strain and system hold promise for the industrial production of epothilone B and its derivatives.

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Liu, Jiaqi, Haibo Zhou, Zhiyu Yang, Xue Wang, Hanna Chen, Lin Zhong, Wentao Zheng, et al. "Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria." Nature Communications 12, no. 1 (July 23, 2021). http://dx.doi.org/10.1038/s41467-021-24645-0.

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AbstractHeterologous expression of biosynthetic gene clusters (BGCs) avails yield improvements and mining of natural products, but it is limited by lacking of more efficient Gram-negative chassis. The proteobacterium Schlegelella brevitalea DSM 7029 exhibits potential for heterologous BGC expression, but its cells undergo early autolysis, hindering further applications. Herein, we rationally construct DC and DT series genome-reduced S. brevitalea mutants by sequential deletions of endogenous BGCs and the nonessential genomic regions, respectively. The DC5 to DC7 mutants affect growth, while the DT series mutants show improved growth characteristics with alleviated cell autolysis. The yield improvements of six proteobacterial natural products and successful identification of chitinimides from Chitinimonas koreensis via heterologous expression in DT mutants demonstrate their superiority to wild-type DSM 7029 and two commonly used Gram-negative chassis Escherichia coli and Pseudomonas putida. Our study expands the panel of Gram-negative chassis and facilitates the discovery of natural products by heterologous expression.

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Romen, Fabian, Simone Reinhardt, and Dieter Jendrossek. "Thermotolerant poly(3-hydroxybutyrate)-degrading bacteria from hot compost and characterization of the PHB depolymerase of Schlegelella sp. KB1a." Archives of Microbiology 182, no. 2-3 (August 31, 2004). http://dx.doi.org/10.1007/s00203-004-0684-2.

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17

Wang, Huimin, Junheng Liang, Qianwen Yue, Long Li, Yan Shi, Guosong Chen, Yue-zhong Li, et al. "Engineering the acyltransferase domain of epothilone polyketide synthase to alter the substrate specificity." Microbial Cell Factories 20, no. 1 (April 21, 2021). http://dx.doi.org/10.1186/s12934-021-01578-3.

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Abstract Background Polyketide synthases (PKSs) include ketone synthase (KS), acyltransferase (AT) and acyl carrier protein (ACP) domains to catalyse the elongation of polyketide chains. Some PKSs also contain ketoreductase (KR), dehydratase (DH) and enoylreductase (ER) domains as modification domains. Insertion, deletion or substitution of the catalytic domains may lead to the production of novel polyketide derivatives or to the accumulation of desired products. Epothilones are 16-membered macrolides that have been used as anticancer drugs. The substrate promiscuity of the module 4 AT domain of the epothilone PKS (EPOAT4) results in production of epothilone mixtures; substitution of this domain may change the ratios of epothilones. In addition, there are two dormant domains in module 9 of the epothilone PKS. Removing these redundant domains to generate a simpler and more efficient assembly line is a desirable goal. Results The substitution of module 4 drastically diminished the activity of epothilone PKS. However, with careful design of the KS-AT linker and the post-AT linker, replacing EPOAT4 with EPOAT2, EPOAT6, EPOAT7 or EPOAT8 (specifically incorporating methylmalonyl-CoA (MMCoA)) significantly increased the ratio of epothilone D (4) to epothilone C (3) (the highest ratio of 4:3 = 4.6:1), whereas the ratio of 4:3 in the parental strain Schlegelella brevitalea 104-1 was 1.4:1. We also obtained three strains by swapping EPOAT4 with EPOAT3, EPOAT5, or EPOAT9, which specifically incorporate malonyl-CoA (MCoA). These strains produced only epothilone C, and the yield was increased by a factor of 1.8 compared to that of parental strain 104-1. Furthermore, mutations of five residues in the AT domain identified Ser310 as the critical factor for MMCoA recognition in EPOAT4. Then, the mutation of His308 to valine or tyrosine combined with the mutation of Phe310 to serine further altered the product ratios. At the same time, we successfully deleted the inactive module 9 DH and ER domains and fused the ΨKR domain with the KR domain through an ~ 25-residue linker to generate a productive and simplified epothilone PKS. Conclusions These results suggested that the substitution and deletion of catalytic domains effectively produces desirable compounds and that selection of the linkers between domains is crucial for maintaining intact PKS catalytic activity.

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Journal articles on the topic 'Schlegelella'

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