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1

Stępniak, Kinga M., Natalia Niedźwiecka, Maciej Szewczyk, and Robert W. Mysłajek. "Scent marking in wolves Canis lupus inhabiting managed lowland forests in Poland." Mammal Research 65, no. 4 (June 13, 2020): 629–38. http://dx.doi.org/10.1007/s13364-020-00514-x.

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Abstract In wolves Canis lupus, scent marking plays an important role in territory defence. In Europe, studies on patterns of scent marking in wolves have mostly been conducted in mountains or primeval forests, but since these areas are characterised by low human activity, the impact of people on this behaviour has been neglected. We conducted a study that combined genetic methods with an analysis of the spatial distribution of wolf territory markings in lowland managed forests with high human activity. We found that scent markings are deposited by all members of wolf family groups. Wolves most intensively marked crossroads and their vicinity, especially on roads only accessible for four-wheel drive cars. Our study provides further evidence that crossroads of forest roads play a crucial role in wolf scent marking. The results of our study may be useful during inventories of wolf populations based on collecting indirect signs of their presence or non-invasive genetic sampling.
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2

Paquet, Paul C. "Scent-marking behavior of sympatric wolves (Canis lupus) and coyotes (C. latrans) in Riding Mountain National Park." Canadian Journal of Zoology 69, no. 7 (July 1, 1991): 1721–27. http://dx.doi.org/10.1139/z91-240.

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The objectives of this study were to describe the scent-marking behavior of sympatric wolves and coyotes, and determine whether scent marking has an interspecific behavioral significance and, if so, whether it is involved in maintaining ecological separation of the two species. The hypotheses tested were that coyotes travelling within active wolf areas reduce their frequency of marking and avoid wolf scent marks, and that wolves react to sympatric coyotes as transgressing conspecifics, and increase their scent marking. Marking by the two species was similar, both canids using the same scent mounds. Coyotes did not avoid areas marked by wolves, nor did they minimize evidence of their own activity. Coyotes increased their marking significantly in response to wolves. Wolves, however, did not respond to marks by coyotes.
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3

Gorman, M. L. "Scent marking strategies in mammals." Revue suisse de zoologie. 97 (1990): 3–29. http://dx.doi.org/10.5962/bhl.part.79722.

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4

Berthoud, D. "Investigating scent marking in dogs." Veterinary Record 160, no. 21 (May 26, 2007): 744. http://dx.doi.org/10.1136/vr.160.21.744-d.

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Berthoud, D. "Investigating scent marking in dogs." Veterinary Record 158, no. 16 (April 22, 2006): 572. http://dx.doi.org/10.1136/vr.158.16.572-c.

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6

Ferkin, Michael H., and Nicholas J. Hobbs. "Effect of protein content of the diet on scent marking and over-marking behavior in meadow voles, Microtus pennsylvanicus." Behaviour 148, no. 9-10 (2011): 1027–44. http://dx.doi.org/10.1163/000579511x588083.

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AbstractDifferences in the protein content of individuals may affect their response to signals produced by opposite-sex conspecifics and how opposite-sex conspecifics respond to the individual's own signals. Many terrestrial mammals use over-marks to communicate with potential mates. In this study, we determined whether over-marking behavior is affected by the protein content of the top- and bottom-scent donors of an over-mark. We tested two hypotheses by performing two experiments on meadow voles, Microtus pennsylvanicus, in which the top- or bottom-scent donors of an over-mark were fed a diet containing either 9, 13, or 22% protein; these protein concentrations are similar to those that voles may consume in free-living populations. In experiment 1, we varied the protein content of the top-scent voles but not that of the bottom-scent donor. We tested the hypothesis that top-scent donor voles fed a diet high in protein content deposit more scent marks and more over-marks than do top-scent donor voles fed diets lower in protein content. In experiment 2, the top-scent voles were fed a 22% protein diet but the protein content of the diet of the bottom-scent donor varied. We tested the hypothesis that the top-scent donor will deposit more scent marks if the bottom-scent vole was fed a diet high in protein content than if it was fed a diet lower in protein content. Protein content of the top-scent vole's diet did not affect the number of scent marks and over-marks it deposited. Likewise, the protein content of the bottom-scent vole did not affect the number of scent marks and over-marks deposited by the top-scent vole.
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7

Taylor, Benjamin J., Erik V. Nordheim, Teresa I. Schueller, and Robert L. Jeanne. "Recruitment in Swarm-Founding Wasps:Polybia occidentalisDoes not Actively Scent-Mark Carbohydrate Food Sources." Psyche: A Journal of Entomology 2011 (2011): 1–7. http://dx.doi.org/10.1155/2011/378576.

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Scent marking food resources is expected to enhance foraging efficiency reducing search time. Many social bees exhibit this behavior, but scent-marking is absent in social wasps, except forVespa mandarinia. We tested for scent marking in the swarm-founding wasp,Polybia occidentalis. This wasp has moderately large colonies and utilizes resources that are concentrated in time and space, making scent marking profitable. Also, this wasp uses chemical markings to lead nestmates to a new nest site during swarm emigration, making it possible that it could use the same behavior to recruit nestmates to a food source. Foragers from 11 colonies were given a choice between a previously visited feeder and an unvisited one, both containing a rich, unscented sucrose solution. There was no difference in the number of visits to the two treatments. However, some individuals chose the feeder on one side more often. We conclude that foragers of this species of wasp do not use odor marks left behind by nestmates to find food, but they do exhibit the tendency, when returning to a food source that has not been depleted, to choose a resource based on its relative position, presumably by using visual cues.
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8

Rafiq, Kasim, Neil R. Jordan, Carlo Meloro, Alan M. Wilson, Matthew W. Hayward, Serge A. Wich, and John W. McNutt. "Scent-marking strategies of a solitary carnivore: boundary and road scent marking in the leopard." Animal Behaviour 161 (March 2020): 115–26. http://dx.doi.org/10.1016/j.anbehav.2019.12.016.

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9

Elwell, Emily J., David Walker, and Stefano Vaglio. "Sexual Dimorphism in Crowned Lemur Scent-Marking." Animals 11, no. 7 (July 14, 2021): 2091. http://dx.doi.org/10.3390/ani11072091.

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Primates are traditionally considered to have a poor sense of smell. However, olfaction is important for non-human primates as demonstrated by conspicuous scent-marking behaviours in lemurs. We studied two pairs (n = 4) of crowned lemurs (Eulemur coronatus) housed at Colchester and Twycross zoos (UK) by combining behavioural observations and chemical analyses of scent-marks and glandular swabs. We recorded observations of olfactory behaviours for 201 h using instantaneous scan sampling. We investigated the volatile compounds of ano-genital odour secretions (n = 16) using solid-phase microextraction and gas chromatography-mass spectrometry. Males scent-marked most frequently, displaying ano-genital marking for allomarking, head marking for countermarking and wrist marking in specific areas of the enclosure. Females displayed ano-genital marking, predominantly on feeding devices. We detected a total of 38 volatile components in all male ano-genital scent-marks and 26 in all female samples of ano-genital odour secretions, including a series of esters, aldehydes, ketones, alcohols, terpenes, volatile fatty acids and hydrocarbons that have been identified in odour profiles of other primates. In conclusion, we found sexual dimorphism in crowned lemur scent-marking. Male head and wrist marking behaviours might play defensive territorial functions, while ano-genital marking would be related to socio-sexual communication as chemical mate-guarding. Female ano-genital marking might be involved in resource defense.
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10

Gorman, M. L., and M. G. L. Mills. "Scent marking strategies in hyaenas (Mammalia)." Journal of Zoology 202, no. 4 (August 20, 2009): 535–47. http://dx.doi.org/10.1111/j.1469-7998.1984.tb05050.x.

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11

Ferron, Jean, and Jean-Pierre Ouellet. "Behavioural context and possible function of scent marking by cheek rubbing in the red squirrel (Tamiasciurus hudsonicus)." Canadian Journal of Zoology 67, no. 7 (July 1, 1989): 1650–53. http://dx.doi.org/10.1139/z89-236.

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A contextual analysis of scent marking by cheek rubbing in wild red squirrels (Tamiasciurus hudsonicus), based on the study of behavioural time budgets and sequences, is presented. Scent-marking rates do not differ significantly between adult males and females. Comparison of the relative frequency of occurrence of each behaviour pattern in a scent-marking context with the relative frequency of these patterns in all other contexts reveals that locomotion, alertness, grooming, food carrying, and gnawing have a higher probability of occurrence when there is cheek rubbing. These results concur with earlier findings in captivity. Contextual analysis in the wild clearly indicates low association of cheek rubbing with social context and greater use of this behaviour at grooming and resting sites and along the path used by the marking animal. This suggests that cheek rubbing is mainly self-oriented to maintain the animal's familiarity with its home range. This scent-marking behaviour may also be used to advertise occupancy of a given territory to conspecifics.
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12

Hébert, Paule, and Cyrille Barrette. "Experimental demonstration that scent marking can predict dominance in the woodchuck, Marmota monax." Canadian Journal of Zoology 67, no. 3 (March 1, 1989): 575–78. http://dx.doi.org/10.1139/z89-082.

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Scent marking is known to be related to dominance in mammals. Here we ask whether the isolated scent from the oral glands of woodchucks (Marmota monax) can advertise dominance. The scent of an individual was presented to a conspecific before the two met and could establish a dominance–subordination relationship. For all 19 dyads that would later express aggressive dominance, the individual pre-encounter rate of scent marking was the same no matter the sex, composition of the dyad, or the future status of the individual. However, when they were presented with scent marks of a conspecific (before meeting the marker), future subordinates marked the scent of future dominants more than vice versa (Mann–Whitney U-test, Z = 2.246, [Formula: see text]). The status of members of dyads was accurately predicted from the pre-encounter marking performance in 14 of the 19 dyads (χ2 = 3.368; 0.10 > p > 0.05). This suggests that scent, by itself, conveys information on the dyadic dominance status of an individual relative to the receiver of the olfactory signal.
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13

Feldman, Hilary N. "Methods of scent marking in the domestic cat." Canadian Journal of Zoology 72, no. 6 (June 1, 1994): 1093–99. http://dx.doi.org/10.1139/z94-147.

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Carnivores use various scent-marking methods. Semi-feral domestic cats (Felis silvestris catus) were observed to use the same means as their wild counterparts. Adult males performed most urine spray marking. Cats scratched tree bark, producing a visual mark, and probably used trees both as markers and for claw sharpening. Most scratching trees were located along frequently used paths rather than along territorial boundaries or scattered randomly throughout a home range. Bark consistency affected the tree species that were scratched, with soft bark preferred. Although deposition of faeces and urine was recorded, there was no clear evidence for their use as territorial markers; cats primarily eliminated away from the core area of the home range. Most faeces were buried, although exposed deposits were also observed. Cats also rubbed against objects, probably using glandular secretions from the face and tail areas to scent mark. Males rubbed objects more than females, and males scent marked more. Individual males may use different means of scent marking. Scent marking in this study supports the idea that cats do not defend territories, instead patrolling and reinforcing marks throughout a looser home range. The suggestion has been made that different forms of marking may serve separate signalling functions.
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14

Mech, S. G., and M. H. Ferkin. "SCENT MARKING IN MEADOW VOLES AND PRAIRIE VOLES: A TEST OF THREE HYPOTHESES." Behaviour 138, no. 11-12 (2001): 1319–36. http://dx.doi.org/10.1163/156853901317367618.

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AbstractMost terrestrial mammals deposit scent marks to communicate with conspecifics. We examined the scent marking behaviour of meadow voles and prairie voles, species with different mating systems and social organizations, to determine whether voles scent mark according to the 'targeting' response, the 'avoidance' response, or the 'shotgun' response. The targeting response occurs when the second scent donor deposits more of its scent marks in an area marked by the first scent donor than in an unscented area. The avoidance response occurs when the second scent donor deposits more of its scent marks in an unscented area than in an area marked by the first scent donor. The shotgun response occurs when the second scent donor deposits a similar number of its scent marks in an area containing scent marks of a conspecific and in an area containing no conspecific scent marks. We allowed voles simultaneous access to an arena containing two arms: one of the arms was scented by a conspecific and the other arm was unscented. We recorded the number of marks deposited by the voles in each arm and the amount of time they spent investigating marks deposited previously in the scented arm. Our data provide no support for the avoidance response, but provide support for the shotgun response and the target response. Species and sex differences in the scent marking behaviours of voles when they encounter the scent marks of conspecifics are discussed within the framework that scent marking responses depend on the voles' social organization and mating system, and that these responses may reflect the tactics males and females use to attract mates and compete with same-sex conspecifics.
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15

Charlton, B. D., M. A. Owen, H. Zhang, and R. R. Swaisgood. "Scent anointing in mammals: functional and motivational insights from giant pandas." Journal of Mammalogy 101, no. 2 (February 22, 2020): 582–88. http://dx.doi.org/10.1093/jmammal/gyaa014.

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Abstract Although several mammals impregnate their fur with environmental odors, a phenomenon termed scent anointing or rubbing, the functional relevance of this behavior often is unclear. One theory is that scent anointing could be a form of scent matching with environmental odors to signal competitiveness and home range occupation. In this study we presented giant pandas with a range of odors to determine whether scent matching could provide a functional explanation for scent anointing in this species. We found that only a musk-based perfume elicited significantly more scent-anointing and scent-marking behavior than control. Males were also significantly more likely to scent-anoint and scent-mark than females. A preference for anointing, but not scent marking, when presented with peppermint (an insecticide) also was revealed. Our results suggest that giant pandas differentially scent-anoint with foreign odors to signal home range occupation, and possibly to repel ectoparasites. We also highlight how chemical signaling of resource-holding potential is likely to play an important role in determining competitive interactions between adult male giant pandas.
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16

Becker, Elizabeth A., Frank R. Castelli, Christine N. Yohn, Lindsey Spencer, and Catherine A. Marler. "Species differences in urine scent-marking and counter-marking in Peromyscus." Behavioural Processes 146 (January 2018): 1–9. http://dx.doi.org/10.1016/j.beproc.2017.10.011.

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17

Ouellet, J. P., and J. Ferron. "Scent-Marking Behavior by Woodchucks (Marmota monax)." Journal of Mammalogy 69, no. 2 (May 20, 1988): 365–68. http://dx.doi.org/10.2307/1381388.

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18

Bowyer, R. T., and D. W. Kitchen. "Significance of Scent-Marking by Roosevelt Elk." Journal of Mammalogy 68, no. 2 (May 26, 1987): 418–23. http://dx.doi.org/10.2307/1381489.

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19

Rylands, Anthony B. "Tree-gouging and scent-marking by marmosets." Animal Behaviour 33, no. 4 (November 1985): 1365–67. http://dx.doi.org/10.1016/s0003-3472(85)80202-5.

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20

Gosling, L. M. "Scent marking in an antelope lek territory." Animal Behaviour 35, no. 2 (April 1987): 620–22. http://dx.doi.org/10.1016/s0003-3472(87)80298-1.

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21

Mertl-Millhollen, Anne S., Patricia A. Goodmann, and Erich Klinghammer. "Wolf scent marking with raised-leg urination." Zoo Biology 5, no. 1 (1986): 7–20. http://dx.doi.org/10.1002/zoo.1430050103.

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22

Heymann, Eckhard W. "Scent marking strategies of new world primates." American Journal of Primatology 68, no. 6 (2006): 650–61. http://dx.doi.org/10.1002/ajp.20258.

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23

Hohwieler, Katrin, Frank Rosell, and Martin Mayer. "Scent-marking behavior by subordinate Eurasian beavers." Ethology 124, no. 8 (June 19, 2018): 591–99. http://dx.doi.org/10.1111/eth.12762.

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24

Emilio, A. H., and D. W. Macdonald. "Social Significance of Scent Marking in Capybaras." Journal of Mammalogy 75, no. 2 (May 31, 1994): 410–15. http://dx.doi.org/10.2307/1382561.

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25

Freeman, N. J., G. M. Pasternak, T. L. Rubi, L. Barrett, and S. P. Henzi. "Evidence for scent marking in vervet monkeys?" Primates 53, no. 3 (March 9, 2012): 311–15. http://dx.doi.org/10.1007/s10329-012-0304-8.

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26

Thomas, Shawn A., and Jerry O. Wolff. "Scent Marking in Voles: A Reassessment of Over Marking, Counter Marking, and Self-Advertisement." Ethology 108, no. 1 (January 2002): 51–62. http://dx.doi.org/10.1046/j.1439-0310.2002.00753.x.

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27

Allen, Maximilian L., Heiko U. Wittmer, Emmarie P. Alexander, and Christopher C. Wilmers. "Ontogeny of scent marking behaviours in an apex carnivore." Behaviour 159, no. 3-4 (October 1, 2021): 339–50. http://dx.doi.org/10.1163/1568539x-bja10127.

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Abstract Puma (Puma concolor) communication with conspecifics is via indirect scent marking behaviours that are important for individuals to advertise their territory and reproductive status, but little is known about how the behaviours develop with age. To examine the development of scent marking behaviours, we monitored the behaviours of adult pumas and dependent kittens. Based on video recordings, we found that the frequency of puma communication behaviours significantly changed over time. Kittens exhibited olfactory investigation more frequently as they aged, but kittens generally did not exhibit scent marking behaviours. Kittens travel with their mothers until they disperse, so there is no need to establish territories or advertise availability to mate, but kittens are at risk of injury or mortality from other pumas. It is possible that there is no functional need for dependent kittens to scent mark until they mature, but there is a need for frequent use of investigative behaviours.
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28

Jordan, Neil R., Marta B. Manser, Francis Mwanguhya, Solomon Kyabulima, Peter Rüedi, and Michael A. Cant. "Scent marking in wild banded mongooses: 1. Sex-specific scents and overmarking." Animal Behaviour 81, no. 1 (January 2011): 31–42. http://dx.doi.org/10.1016/j.anbehav.2010.07.010.

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29

Lin, Yi, Qunxiu Liu, Ningning Song, Endi Zhang, and Min Chen. "Chinese red panda (Ailurus styani) has stable individual-level laterality of anogenital rubbing." Behaviour 159, no. 3-4 (September 24, 2021): 393–402. http://dx.doi.org/10.1163/1568539x-bja10130.

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Abstract Behavioural laterality was widely discovered in both vertebrates and invertebrates. However, reports of behavioural laterality in scent-marking are scarce and focused on limb preference during scent-marking. In this study, we observed another scent-marking behaviour, anogenital rubbing, which involved whole-body movement, in zoo-housed Chinese red pandas. We recorded the moving direction of the buttocks when initiating anogenital rubbing. Our results showed that three of our subjects were explicitly left-biased when initiating anogenital rubbing and the other one showed more left initiation although there was no statistical significance. Besides, the laterality was consistent across the whole observational period. This is the first report of laterality in anogenital rubbing, perhaps indicating hemispheric specialization for chemical communication in the Chinese red panda.
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30

Morales-González, Ana, Héctor Ruíz-Villar, Arpat Ozgul, Nino Maag, and Gabriele Cozzi. "Group size and social status affect scent marking in dispersing female meerkats." Behavioral Ecology 30, no. 6 (August 2, 2019): 1602–10. http://dx.doi.org/10.1093/beheco/arz124.

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Abstract Many animal species use scent marks such as feces, urine, and glandular secretions to find mates, advertise their reproductive status, and defend an exclusive territory. Scent marking may be particularly important during dispersal, when individuals emigrate from their natal territory searching for mates and a new territory to settle and reproduce. In this study, we investigated the scent-marking behavior of 30 dispersing female meerkats (Suricata suricatta) during the three consecutive stages of dispersal—emigration, transience, and settlement. We expected marking patterns to differ between dispersal stages, depending on social circumstances such as presence of unrelated mates and social status of the individuals within each dispersing coalition and also to be influenced by water and food availability. We showed that defecation probability increased with group size during the settlement stage, when newly formed groups are expected to signal their presence to other resident groups. Urination probability was higher in subordinate than in dominant individuals during each of the three dispersal stages and it decreased overall as the dispersal process progressed. Urine may, thus, be linked to advertisement of the social status within a coalition. Anal marking probability did not change across dispersal stages but increased with the presence of unrelated males and was higher in dominants than in subordinates. We did not detect any effect of rain or foraging success on defecation and urination probability. Our results suggest that feces, urine, and anal markings serve different communication purposes (e.g., within and between-group communication) during the dispersal process.
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31

Johnston, Robert E., Evan S. Sorokin, and Michael H. Ferkin. "Scent Counter-marking by Male Meadow Voles: Females Prefer the Top-scent Male." Ethology 103, no. 6 (April 26, 2010): 443–53. http://dx.doi.org/10.1111/j.1439-0310.1997.tb00159.x.

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32

Jordan, Neil R., Krystyna A. Golabek, Peter J. Apps, Geoffrey D. Gilfillan, and John W. McNutt. "Scent-Mark Identification and Scent-Marking Behaviour in African Wild Dogs (Lycaon pictus)." Ethology 119, no. 8 (June 21, 2013): 644–52. http://dx.doi.org/10.1111/eth.12105.

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33

Kimura, Rikako. "Volatile substances in feces, urine and urine-marked feces of feral horses." Canadian Journal of Animal Science 81, no. 3 (September 1, 2001): 411–20. http://dx.doi.org/10.4141/a00-068.

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The identity and amount of volatile substances in the feces, urine and feces scent-marked with urine (i.e., feces mixed with urine) of feral horses was determined by acid/steam distillation and gas chromatography–mass spectrometry. The frequency of excretion and scent marking, as evaluated in the breeding and non-breeding seasons, showed clear evidence of seasonal behavioral differences. The concentration of each substance (fatty acids, alcohols, aldehydes, phenols, amines and alkanes) in the feces differed according to maturity, sex and stage in the reproductive process. They had a characteristic chemical fingerprint. Although the levels of tetradecanoic and hexadecanoic acids in the feces of estrous mares were significantly higher than the respective levels in the feces of non-estrous mares, in the case of scent-marked feces by stallions, the levels of them in the feces from estrous mares had decreased to levels similar to those in non-estrous mares. The concentration of these substances in mares were not significantly different. The presence of a high concentration of cresols in the urine of stallions in the breeding season suggests that one role of scent marking by stallions is masking the odor of the feces produced by mares. Key words: Odors (volatile), excrement, scent-marking, masking, horse (feral), (releaser) pheromone
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34

Gromov, V. S. "Scent marking in gerbils and its possible functions." Russian Journal of Theriology 14, no. 1 (June 3, 2015): 113–26. http://dx.doi.org/10.15298/rusjtheriol.14.1.06.

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35

Johnston, Robert E. "Scent Marking by Male Golden Hamsters (Mesocricetus auratus)." Zeitschrift für Tierpsychologie 37, no. 1 (April 26, 2010): 75–98. http://dx.doi.org/10.1111/j.1439-0310.1975.tb01128.x.

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36

Feistner, Anna T. C. "Scent Marking in Mandrills, Mandrillus sphinx." Folia Primatologica 57, no. 1 (1991): 42–47. http://dx.doi.org/10.1159/000156563.

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37

Conover, Greta K., and John L. Gittleman. "Scent-marking in captive red pandas (Ailurus fulgens)." Zoo Biology 8, no. 2 (1989): 193–205. http://dx.doi.org/10.1002/zoo.1430080210.

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38

Mertl-millhollen, Anne S. "Scent marking as resource defense by femaleLemur catta." American Journal of Primatology 68, no. 6 (2006): 605–21. http://dx.doi.org/10.1002/ajp.20255.

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39

Sharpe, Lynda L. "Handstand scent marking: height matters to dwarf mongooses." Animal Behaviour 105 (July 2015): 173–79. http://dx.doi.org/10.1016/j.anbehav.2015.04.019.

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40

Tennant, Leeanne E., Emilie F. Rissman, and F. H. Bronson. "Scent marking in the musk shrew (Suncus murinus)." Physiology & Behavior 39, no. 6 (January 1987): 677–80. http://dx.doi.org/10.1016/0031-9384(87)90249-6.

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41

David Smith, James L., Charles McDougal, and Dale Miquelle. "Scent marking in free-ranging tigers,Panthera tigris." Animal Behaviour 37 (January 1989): 1–10. http://dx.doi.org/10.1016/0003-3472(89)90001-8.

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42

Ruibal, Monica, Rod Peakall, Andrew Claridge, and Karen Firestone. "Field-based evaluation of scat DNA methods to estimate population abundance of the spotted-tailed quoll (Dasyurus maculatus), a rare Australian marsupial." Wildlife Research 36, no. 8 (2009): 721. http://dx.doi.org/10.1071/wr09086.

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Context. DNA extracted non-invasively from remotely collected scat samples has been used successfully to enumerate populations of a few endangered mammal species. However, scat DNA surveys relying on scent-marking behaviours need to identify if age- or sex-specific variations or seasonal changes in scat scent-marking patterns affect population estimates. Furthermore, owing to the low quantity and quality of scat DNA, a thorough assessment of the technique is needed when it is applied to different species to ensure that individual identification is reliable. Aims. In the current study, microsatellite genetic profiles derived from 208 remotely collected scats of the spotted-tailed quoll (Dasyurus maculatus), a rare Australian marsupial carnivore, were compared with DNA profiles from tissue of 22 live-trapped individuals from the same study area to critically assess the reliability of the non-invasive method to estimate population abundance. Methods. Scat samples were collected at scent-marking sites over 4 consecutive months (April–July 2005), 7 weeks of which overlapped with the trapping program to allow direct comparisons of population estimates. Key results. Combining a multiple-tubes approach with error checking analyses provided reliable genetic tags and resulted in the detection of the majority of the live-trapped population (18 of 22 individuals). Ten additional individuals not known from trapping were also observed from scat DNA. A longer-term sampling regime was required for scats than for trapping to allow direct detection of a large proportion of the population and to provide a comparable population estimate. Critically, the 4-month scat collection period highlighted the importance of performing scat surveys during the mating season when scat scent marking is more frequent, and to avoid sex and age biases in scat marking patterns. Implications. Non-invasive scat DNA sampling methods that rely on scent-marking behaviours need to consider the duration of the sampling period and temporal differences in behaviours by the sexes and age groups to ensure that meaningful population estimates are achieved.
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Leu, Stephan T., Grant Jackson, John F. Roddick, and C. Michael Bull. "Lizard movement tracks: variation in path re-use behaviour is consistent with a scent-marking function." PeerJ 4 (March 22, 2016): e1844. http://dx.doi.org/10.7717/peerj.1844.

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Individual movement influences the spatial and social structuring of a population. Animals regularly use the same paths to move efficiently to familiar places, or to patrol and mark home ranges. We found that Australian sleepy lizards (Tiliqua rugosa), a monogamous species with stable pair-bonds, repeatedly used the same paths within their home ranges and investigated whether path re-use functions as a scent-marking behaviour, or whether it is influenced by site familiarity. Lizards can leave scent trails on the substrate when moving through the environment and have a well-developed vomeronasal system to detect and respond to those scents. Path re-use would allow sleepy lizards to concentrate scent marks along these well-used trails, advertising their presence. Hypotheses of mate attraction and mating competition predict that sleepy lizard males, which experience greater intra-sexual competition, mark more strongly. Consistent with those hypotheses, males re-used their paths more than females, and lizards that showed pairing behaviour with individuals of the opposite sex re-used paths more than unpaired lizards, particularly among females. Hinterland marking is most economic when home ranges are large and mobility is low, as is the case in the sleepy lizard. Consistent with this strategy, re-used paths were predominantly located in the inner 50% home range areas. Together, our detailed movement analyses suggest that path re-use is a scent marking behaviour in the sleepy lizard. We also investigated but found less support for alternative explanations of path re-use behaviour, such as site familiarity and spatial knowledge. Lizards established the same number of paths, and used them as often, whether they had occupied their home ranges for one or for more years. We discuss our findings in relation to maintenance of the monogamous mating system of this species, and the spatial and social structuring of the population.
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44

Arnold, J., C. D. Soulsbury, and S. Harris. "Spatial and behavioral changes by red foxes (Vulpes vulpes) in response to artificial territory intrusion." Canadian Journal of Zoology 89, no. 9 (September 2011): 808–15. http://dx.doi.org/10.1139/z11-069.

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Urine marking is thought to play a pivotal role in territory demarcation by red foxes ( Vulpes vulpes (L., 1758)), but little is known about how individuals respond to alien scent marks, and whether there are sex-related differences in territorial defense. We radio-tracked dominant male and female urban foxes before and after synthetic fox urine was applied to approximately a third of their territories and compared spatial and behavioral reactions both before and after scent application and with foxes on territories where no urine was applied. Home-range boundaries of male foxes shifted towards the scent-marked area, but this change did not affect the total territory size. Larger males shifted their home ranges to a greater degree than small males. Scent application did not affect total activity, but males spent more time in the scent-marked area. Behaviors such as distance moved per night and speed of movement did not differ before and after application, but foxes searched a greater percentage of their home range each night following scent marking. Females showed no significant spatial or behavioral response to the synthetic scent marks. Overall, responses of foxes to synthetic scent marks were male-biased and related to changes in space use rather than movement behaviors.
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Adams, Craig A., R. Terry Bowyer, Jan E. Rowell, William E. Hauer, and Jonathan A. Jenks. "Scent marking by male caribou: an experimental test of rubbing behavior." Rangifer 21, no. 1 (March 1, 2001): 21. http://dx.doi.org/10.7557/2.21.1.1524.

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We studied scent marking by adult male caribou (Rangifer tarandus) during rut in September 1998 at the Large Animal Research Station in Fairbanks, Alaska, USA. We used an experimental approach involving two captive groups of two males each to test for effects of social status, tree size, texture, and scent on rubbing behavior by caribou. Dominant males did not rub more often or for a longer duration than subordinates. Caribou rubbed trees with smaller diameters more often than large-diameter trees. Males preferred trees with bark for rubbing to those trees with their bark removed prior to the experiment. Caribou exhibited no preference for posts with pine-oil applied compared with posts without that aromatic scent. We hypothesize that rubbing of trees by male caribou is related to synchronization or priming of estrus in females, but more research is needed to test that potential function of scent marking.
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Arakawa, Hiroyuki, Keiko Arakawa, D. Caroline Blanchard, and Robert J. Blanchard. "Social features of scent-donor mice modulate scent marking of C57BL/6J recipient males." Behavioural Brain Research 205, no. 1 (December 2009): 138–45. http://dx.doi.org/10.1016/j.bbr.2009.07.007.

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47

Bowyer, R. Terry, Victor Van Ballenberghe, and Karen R. Rock. "Scent marking by Alaskan moose: characteristics and spatial distribution of rubbed trees." Canadian Journal of Zoology 72, no. 12 (December 1, 1994): 2186–92. http://dx.doi.org/10.1139/z94-292.

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We studied scent marking (rubbing of trees) in Alaskan moose (Alces alces gigas) in interior Alaska during 1989. Pole-sized trees were stripped of bark and rubbed by adult female and adult male moose; marking by females occurred during the peak of rut (late September – early October) when most females were in estrus, whereas marking by males was in late rut (mid-October – November). Moose selected white spruce (Picea glauca) as well as trees with particular physical characteristics for marking. The tops of 18.5% of 54 trees marked by moose were dead, whereas only 0.5% of 201 trees available for marking had dead tops. The distribution of scent-marked trees on rutting grounds was not spatially clumped. We hypothesize that rubbing of trees by females advertises their estrus, and that rubbing by males late in rut serves to attract females not successfully bred early in rut and may help prime estrus in these females.
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48

Kappeler, Petter M. "Social status and scent-marking behaviour in Lemur catta." Animal Behaviour 40, no. 4 (October 1990): 774–76. http://dx.doi.org/10.1016/s0003-3472(05)80706-7.

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Green, Michael J. B. "Scent-marking in the Himalayan musk deer (Moschus chrysogaster)." Journal of Zoology 1, no. 4 (March 1987): 721–37. http://dx.doi.org/10.1111/j.1096-3642.1987.tb00752.x.

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Arakawa, Hiroyuki, D. Caroline Blanchard, Keiko Arakawa, Christopher Dunlap, and Robert J. Blanchard. "Scent marking behavior as an odorant communication in mice." Neuroscience & Biobehavioral Reviews 32, no. 7 (September 2008): 1236–48. http://dx.doi.org/10.1016/j.neubiorev.2008.05.012.

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