Journal articles: 'Sbp1'

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Otsuka, Taketo, Charmaine Kirkham, Aimee Brauer, Mary Koszelak-Rosenblum, Michael G. Malkowski, and Timothy F. Murphy. "The Vaccine Candidate Substrate Binding Protein SBP2 Plays a Key Role in Arginine Uptake, Which Is Required for Growth of Moraxella catarrhalis." Infection and Immunity 84, no. 2 (November 23, 2015): 432–38. http://dx.doi.org/10.1128/iai.00799-15.

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Moraxella catarrhalisis an exclusively human pathogen that is an important cause of otitis media in children and lower respiratory tract infections in adults with chronic obstructive pulmonary disease. A vaccine to preventM. catarrhalisinfections would have an enormous global impact in reducing morbidity resulting from these infections. Substrate binding protein 2 (SBP2) of an ABC transporter system has recently been identified as a promising vaccine candidate antigen on the bacterial surface ofM. catarrhalis. In this study, we showed that SBP1, -2, and -3 individually bind different basic amino acids with exquisite specificity. We engineered mutants that each expressed a single SBP from this gene cluster and showed in growth experiments that SBP1, -2, and -3 serve a nutritional function through acquisition of amino acids for the bacterium. SBP2 mediates uptake of arginine, a strict growth requirement ofM. catarrhalis. Adherence and invasion assays demonstrated that SBP1 and SBP3 play a role in invasion of human respiratory epithelial cells, consistent with a nutritional role in intracellular survival in the human respiratory tract. This work demonstrates that the SBPs of an ABC transporter system function in the uptake of basic amino acids to support growth ofM. catarrhalis. The critical role of SBP2 in arginine uptake may contribute to its potential as a vaccine antigen.

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Elhodaky, Mostafa, and Alan Diamond. "Selenium-Binding Protein 1 in Human Health and Disease." International Journal of Molecular Sciences 19, no. 11 (November 2, 2018): 3437. http://dx.doi.org/10.3390/ijms19113437.

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Selenium-binding protein 1 (SBP1) is a highly conserved protein that covalently binds selenium. SBP1 may play important roles in several fundamental physiological functions, including protein degradation, intra-Golgi transport, cell differentiation, cellular motility, redox modulation, and the metabolism of sulfur-containing molecules. SBP1 expression is often reduced in many cancer types compared to the corresponding normal tissues and low levels of SBP1 are frequently associated with poor clinical outcome. In this review, the transcriptional regulation of SBP1, the different physiological roles reported for SBP1, as well as the implications of SBP1 function in cancer and other diseases are presented.

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Bhatter, Nupur, Rajan Iyyappan, and Purusharth I. Rajyaguru. "Characterizing mutations in and genetic interactions of RGG-motif translation repressor Sbp1." Wellcome Open Research 3 (August 22, 2018): 102. http://dx.doi.org/10.12688/wellcomeopenres.14709.1.

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Background: Mechanisms of mRNA fate decisions play an important role in determining if a given mRNA will be translated, stored or degraded upon arrival to cytoplasm. Sbp1 is an important RGG-motif containing protein that is implicated in mRNA fate decisions since it can affect mRNA decapping and translation. Sbp1 represses translation by binding eIF4G1 through its RGG-motif and activates decapping when overexpressed. In order to understand the amino acids important for translation repression activity of Sbp1 we performed mutational analysis of Sbp1 combined with assessing its genetic interaction with another RGG-motif protein Scd6. We created two classes of point mutations a) in aromatic residues of the RGG-motif and b) in residues reported to be phosphorylated. Method: Sequence alignment was performed to identify aromatic residues to be mutated based on conservation. Site-directed mutagenesis approach was used to create several point mutations in Sbp1 expressed under galactose-inducible promoter. The mutants were tested for their ability to cause growth defect upon overexpression. The ability of Sbp1 to affect repression activity of other decapping activators was tested using the same growth assay. Results: Mutation of several aromatic residues in the RGG-motif of Sbp1 led to a weak rescue phenotype. However the phospho-mimetic mutants of Sbp1 did not lead to any kind of growth defect rescue. Deletion of another eIF4G1-binding RGG-motif protein Scd6 does not affect ability of Sbp1 to cause growth defect. On the other hand absence of Sbp1 does not affect ability of Dhh1 and Pat1 to repress translation. Conclusion: Based on our growth assay analysis we conclude that mutated aromatic residues contribute marginally to repression activity of Sbp1 whereas phospho-mimetic mutants do not alter ability of Sbp1 to cause growth defect. Interestingly Scd6 does not affect ability of Sbp1 to repress translation, which in turn does not affect Dhh1 and Pat1.

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Bhatter, Nupur, Rajan Iyyappan, Gayatri Mohanan, and Purusharth I. Rajyaguru. "Exploring the role of RRM domains and conserved aromatic residues in RGG motif of eIF4G-binding translation repressor protein Sbp1." Wellcome Open Research 3 (September 17, 2021): 102. http://dx.doi.org/10.12688/wellcomeopenres.14709.3.

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Background: RNA binding proteins play crucial role in determining if a given mRNA will be translated, stored, or degraded. Sbp1 is an RGG-motif containing protein that is implicated in affecting mRNA decapping and translation. Sbp1 represses translation by binding eIF4G1 through its RGG-motif and activates decapping when overexpressed. In this report, we have assessed the genetic interaction of Sbp1 with decapping activators such as Dhh1, Pat1, and Scd6. We have further analyzed the importance of different domains and specific conserved residues of Sbp1 in its ability to cause over-expression mediated growth defect. Method: Sequence alignment was performed to identify conserved aromatic residues to be mutated. Using site-directed mutagenesis several point mutations and domain deletions were created in Sbp1 expressed under a galactose-inducible promoter. The mutants were tested for their ability to cause growth defect upon over-expression. The ability of Sbp1 to affect over-expression mediated growth defect of other decapping activators was tested using growth assay. Live cell imaging was done to study localization of Sbp1 and its RRM-deletion mutants to RNA granules upon glucose starvation. Results: Mutation of several aromatic residues in the RGG-motif and that of the phosphorylation sites in the RRM domain of Sbp1 did not affect the growth defect phenotype. Deletion of another eIF4G1-binding RGG-motif protein Scd6 does not affect the ability of Sbp1 to cause growth defect. Moreover, absence of Sbp1 did not affect the growth defect phenotypes observed upon overexpression of decapping activators Dhh1 and Pat1. Strikingly deletion of both the RRM domains (RRM1 and RRM2) and not the RNP motifs within them compromised the growth defect phenotype. Sbp1 mutant lacking both RRM1 and RRM2 was highly defective in localizing to RNA granules. Conclusion: This study identifies an important role of RRM domains independent of the RNP motif in Sbp1 function.

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Bhatter, Nupur, Rajan Iyyappan, and Purusharth I. Rajyaguru. "Exploring the role of RRM domains and conserved aromatic residues in RGG motif of eIF4G-binding translation repressor protein Sbp1." Wellcome Open Research 3 (February 6, 2020): 102. http://dx.doi.org/10.12688/wellcomeopenres.14709.2.

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Background: Mechanisms of mRNA fate decisions play an important role in determining if a given mRNA will be translated, stored or degraded upon arrival to cytoplasm. Sbp1 is an important RGG-motif containing protein that is implicated in affecting mRNA decapping and translation. Sbp1 represses translation by binding eIF4G1 through its RGG-motif and activates decapping when overexpressed. In this report we have assessed the genetic interaction of Sbp1 with decapping activators such as Dhh1, Pat1 and Scd6. We have further analyzed the importance of different domains and specific conserved residues of Sbp1 in translation repression activity. Method: Sequence alignment was performed to identify conserved aromatic residues to be mutated. Using site-directed mutagenesis several point mutations and domain deletions was created in Sbp1 expressed under a galactose-inducible promoter. The mutants were tested for their ability to cause growth defect upon over-expression. The ability of Sbp1 to affect over expression mediated growth defect of other decapping activators was tested using growth assay. Live cell imaging was done to study localization of Sbp1 and its RRM-deletion mutants to RNA granules upon glucose starvation. Results: Mutation of several aromatic residues in the RGG-motif and that of the phosphorylation sites in the RRM domain of Sbp1 did not affect the growth defect phenotype. Deletion of another eIF4G1-binding RGG-motif protein Scd6 does not affect the ability of Sbp1 to cause growth defect. Moreover, absence of Sbp1 did not affect the growth defect phenotypes observed upon overexpression of decapping activators Dhh1 and Pat1. Strikingly deletion of both the RRM domains (RRM1 and RRM2) and not the RNP motifs within them compromised the growth defect phenotype. Sbp1 mutant lacking both RRM1 and RRM2 was highly defective in localizing to RNA granules. Conclusion: This study identifies an important role of RRM domains independent of RNP motif in Sbp1 repression activity.

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Novoa, Isabel, Mark G. Rush, and Peter D’Eustachio. "Isolated Mammalian and Schizosaccharomyces pombeRan-binding Domains Rescue S. pombe sbp1 (RanBP1) Genomic Mutants." Molecular Biology of the Cell 10, no. 7 (July 1999): 2175–90. http://dx.doi.org/10.1091/mbc.10.7.2175.

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Mammalian Ran-binding protein-1 (RanBP1) and its fission yeast homologue, sbp1p, are cytosolic proteins that interact with the GTP-charged form of Ran GTPase through a conserved Ran-binding domain (RBD). In vitro, this interaction can accelerate the Ran GTPase-activating protein–mediated hydrolysis of GTP on Ran and the turnover of nuclear import and export complexes. To analyze RanBP1 function in vivo, we expressed exogenous RanBP1, sbp1p, and the RBD of each in mammalian cells, in wild-type fission yeast, and in yeast whose endogenous sbp1 gene was disrupted. Mammalian cells and wild-type yeast expressing moderate levels of each protein were viable and displayed normal nuclear protein import.sbp1 − yeast were inviable but could be rescued by all four exogenous proteins. Two RBDs of the mammalian nucleoporin RanBP2 also rescued sbp1 −yeast. In mammalian cells, wild-type yeast, and rescued mutant yeast, exogenous full-length RanBP1 and sbp1p localized predominantly to the cytosol, whereas exogenous RBDs localized predominantly to the cell nucleus. These results suggest that only the RBD of sbp1p is required for its function in fission yeast, and that this function may not require confinement of the RBD to the cytosol. The results also indicate that the polar amino-terminal portion of sbp1p mediates cytosolic localization of the protein in both yeast and mammalian cells.

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Papazzoni, Cesare Andrea, Beatrice Fornaciari, Luca Giusberti, Michela Simonato, and Eliana Fornaciari. "A new definition of the Paleocene Shallow Benthic Zones (SBP) by means of larger foraminiferal biohorizons, and their calibration with calcareous nannofossil biostratigraphy." Micropaleontology 69, no. 4-5 (2023): 363–400. http://dx.doi.org/10.47894/mpal.69.4.02.

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Three key Paleocene deep-sea sections cropping out in northern Italy contain intercalations of calciturbiditic, larger foraminifera-bearing beds derived from shallow water environments. The reconstructed Southern Alps record obtained by splicing the three sections allowed a direct correlation of the shallow benthic SB and calcareous nannofossils CN Zones, leading new data about the Shallow Benthic biozonation for the Danian-Thanetian interval and proposing four new SBP (Shallow Benthic Paleocene) Zones (SBP1-4). Such revision relies on an innovative biostratigraphic approach based on larger foraminiferal biohorizons instead of marker species according to the traditional approach used since the introduction of the SB Zones. Based on our study, the SBP1/SBP2 boundary turns out to be about 2 Ma after the K/Pg crisis, highlighting a quite fast recovery of the complexity among foraminifera.

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Yu-Rice, Yi, Seby L. Edassery, Nicole Urban, Ingegerd Hellstrom, Karl Erik Hellstrom, Youping Deng, Yan Li, and Judith L. Luborsky. "Selenium-Binding Protein 1 (SBP1) autoantibodies in ovarian disorders and ovarian cancer." Reproduction 153, no. 3 (March 2017): 277–84. http://dx.doi.org/10.1530/rep-16-0265.

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Infertility is a risk factor for ovarian cancer (OvCa). The goal was to determine if antibodies to selenium-binding protein 1 (SBP1), an autoantibody we identified in patients with premature ovarian failure (POF), occurs in both infertility and OvCa patients, and thus could be associated with preneoplasia. Anti-SBP1 was measured by immunoassay against recombinant SBP1, in sera from OvCa (n = 41), infertility (n = 92) and control (n = 87) patients. Infertility causes were POF, unexplained, irregular ovulation or endometriosis. The percent of anti-SBP1-positive sera was higher in POF (P = 0.02), irregular ovulation (P = 0.001), unexplained causes (P = 0.02), late (III–IV)-stage OvCa (P = 0.02) but was not significant in endometriosis, benign ovarian tumors/cysts, early stage (I–II) OvCa or uterine cancer compared to healthy controls. Anti-SBP1 was significantly higher in women with serous (P = 0.04) but not non-serous (P = 0.33) OvCa compared to controls. Also, we determined if anti-SBP1 was associated with CA125 or anti-TP53, markers often studied in OvCa. Anti-TP53 and CA125 were measured by established immunoassays. The ability of anti-SBP1 alone to discriminate infertility or OvCa from controls or when combined with anti-TP53 and CA125, to identify OvCa was evaluated by comparing the area under the curve (AUC) in ROC analysis. Anti-SBP1 alone discriminated infertility (AUC = 0.7; P = 0.001) or OvCa (AUC = 0.67; P = 0.03) from controls. The sensitivity and specificity of OvCa identification was increased by combining CA125, anti-TP53 and anti-SBP1 (AUC = 0.96). Therefore, anti-SBP1 occurs in infertile women with POF, ovulatory disturbances or unexplained infertility and in serous OvCa. This suggests an autoimmune process is associated with the development of serous OvCa.

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Bai, Jianan, Junchi Yu, Jintian Wang, Bingyan Xue, Na He, Ye Tian, Lixia Yang, Yipin Wang, Yanyan Wang, and Qiyun Tang. "DNA Methylation of miR-122 Aggravates Oxidative Stress in Colitis Targeting SELENBP1 Partially by p65NF-κB Signaling." Oxidative Medicine and Cellular Longevity 2019 (March 24, 2019): 1–14. http://dx.doi.org/10.1155/2019/5294105.

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Aberrant microRNA (miRNA) expressions contribute to the development and progression of various diseases, including Crohn’s disease (CD). However, the accurate mechanisms of miRNAs in CD are definitely unclear. We employed colonic tissue samples from normal volunteers and CD patients, an acute mice colitis model induced by 2,4,6-trinitro-benzene-sulfonic acid (TNBS), and a cellular oxidative stress model induced by H2O2 in HT-29 cells to determine the effects of oxidative stress on expressions of miR-122, selenium-binding protein 1 (SELENBP1, SBP1), p65 nuclear factor κB (p65NF-κB) signaling, and DNA methylation. We found that SBP1 was mainly located on epithelial cells and was significantly increased in patients with active CD. SBP1 was the target gene of miR-122. miR-122 expression was downregulated while SBP1 expression was upregulated under TNBS-induced colitis or oxidative stress. Pre-miR-122 or siRNA SBP1 (si-SBP1) treatment ameliorated acute TNBS-induced colitis and H2O2-induced oxidative stress. Cotreatment of pre-miR-122 and si-SBP1 enhanced these effects. Besides, pre-miR-122 and si-SBP1 obviously activated the p65NF-κB signaling by phosphorylation of IκBα. Bisulfite sequencing of the CpG islands in the promoter region of miR-122 showed that CpG methylation was significantly increased under oxidative stress. Treating cells with 5′-AZA which was well known as a DNA-demethylating agent significantly increased miR-122 expression. Our results suggest that oxidative stress-induced DNA methylation of miR-122 aggravates colitis targeting SELENBP1 partially by p65NF-κB signaling and may promote the progression of CD.

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Azmi, Ishara, Brian Davies, Christian Dimaano, Johanna Payne, Debra Eckert, Markus Babst, and David J. Katzmann. "Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1." Journal of Cell Biology 172, no. 5 (February 27, 2006): 705–17. http://dx.doi.org/10.1083/jcb.200508166.

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In eukaryotes, the multivesicular body (MVB) sorting pathway plays an essential role in regulating cell surface protein composition, thereby impacting numerous cellular functions. Vps4, an ATPase associated with a variety of cellular activities, is required late in the MVB sorting reaction to dissociate the endosomal sorting complex required for transport (ESCRT), a requisite for proper function of this pathway. However, regulation of Vps4 function is not understood. We characterize Vta1 as a positive regulator of Vps4 both in vivo and in vitro. Vta1 promotes proper assembly of Vps4 and stimulates its ATPase activity through the conserved Vta1/SBP1/LIP5 region present in Vta1 homologues across evolution, including human SBP1 and Arabidopsis thaliana LIP5. These results suggest an evolutionarily conserved mechanism through which the disassembly of the ESCRT proteins, and thereby MVB sorting, is regulated by the Vta1/SBP1/LIP5 proteins.

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Cooke, Brian M., Donna W. Buckingham, Fiona K. Glenister, Kate M. Fernandez, Lawrence H. Bannister, Matthias Marti, Narla Mohandas, and Ross L. Coppel. "A Maurer's cleft–associated protein is essential for expression of the major malaria virulence antigen on the surface of infected red blood cells." Journal of Cell Biology 172, no. 6 (March 6, 2006): 899–908. http://dx.doi.org/10.1083/jcb.200509122.

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The high mortality of Plasmodium falciparum malaria is the result of a parasite ligand, PfEMP1 (P. falciparum) erythrocyte membrane protein 1), on the surface of infected red blood cells (IRBCs), which adheres to the vascular endothelium and causes the sequestration of IRBCs in the microvasculature. PfEMP1 transport to the IRBC surface involves Maurer's clefts, which are parasite-derived membranous structures in the IRBC cytoplasm. Targeted gene disruption of a Maurer's cleft protein, SBP1 (skeleton-binding protein 1), prevented IRBC adhesion because of the loss of PfEMP1 expression on the IRBC surface. PfEMP1 was still present in Maurer's clefts, and the transport and localization of several other Maurer's cleft proteins were unchanged. Maurer's clefts were altered in appearance and were no longer found as close to the periphery of the IRBC. Complementation of mutant parasites with sbp1 led to the reappearance of PfEMP1 on the IRBC surface and the restoration of adhesion. Our results demonstrate that SBP1 is essential for the translocation of PfEMP1 onto the surface of IRBCs and is likely to play a pivotal role in the pathogenesis of P. falciparum malaria.

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Fairlie, W. Douglas, Tim P. Spurck, Joanne E. McCoubrie, Paul R. Gilson, Susanne K. Miller, Geoffrey I. McFadden, Robyn Malby, Brendan S. Crabb, and Anthony N. Hodder. "Inhibition of Malaria Parasite Development by a Cyclic Peptide That Targets the Vital Parasite Protein SERA5." Infection and Immunity 76, no. 9 (June 30, 2008): 4332–44. http://dx.doi.org/10.1128/iai.00278-08.

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ABSTRACT The serine repeat antigen (SERA) proteins of the malaria parasites Plasmodium spp. contain a putative enzyme domain similar to that of papain family cysteine proteases. In Plasmodium falciparum parasites, more than half of the SERA family proteins, including the most abundantly expressed form, SERA5, have a cysteine-to-serine substitution within the putative catalytic triad of the active site. Although SERA5 is required for blood-stage parasite survival, the occurrence of a noncanonical catalytic triad casts doubt on the importance of the enzyme domain in this function. We used phage display to identify a small (14-residue) disulfide-bonded cyclic peptide (SBP1) that targets the enzyme domain of SERA5. Biochemical characterization of the interaction shows that it is dependent on the conformation of both the peptide and protein. Addition of this peptide to parasite cultures compromised development of late-stage parasites compared to that of control parasites or those incubated with equivalent amounts of the carboxymethylated peptide. This effect was similar in two different strains of P. falciparum as well as in a transgenic strain where the gene encoding the related serine-type parasitophorous vacuole protein SERA4 was deleted. In compromised parasites, the SBP1 peptide crosses both the erythrocyte and parasitophorous vacuole membranes and accumulates within the parasitophorous vacuole. In addition, both SBP1 and SERA5 were identified in the parasite cytosol, indicating that the plasma membrane of the parasite was compromised as a result of SBP1 treatment. These data implicate an important role for SERA5 in the regulation of the intraerythrocytic development of late-stage parasites and as a target for drug development.

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He, Xiangwei, Naoyuki Hayashi, Nathan G. Walcott, Yoshiaki Azuma, Thomas E. Patterson, F. Ralf Bischoff, Takeharu Nishimoto, and Shelley Sazer. "The Identification of cDNAs That Affect the Mitosis-to-Interphase Transition in Schizosaccharomyces pombe, Including sbp1, Which Encodes a spi1p-GTP–Binding Protein." Genetics 148, no. 2 (February 1, 1998): 645–56. http://dx.doi.org/10.1093/genetics/148.2.645.

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Abstract Perturbations of the spi1p GTPase system in fission yeast, caused by mutation or overexpression of several regulatory proteins, result in a unique terminal phenotype that includes condensed chromosomes, a wide medial septum, and a fragmented nuclear envelope. To identify potential regulators or targets of the spi1p GTPase system, a screen for cDNAs whose overexpression results in this terminal phenotype was conducted, and seven clones that represent three genes, named med1, med2, and med3 (mitotic exit defect), were identified. Their genetic interaction with the spi1p GTPase system was established by showing that the spi1p guanine nucleotide exchange factor mutant pim1-d1ts was hypersensitive to their overexpression. med1 encodes a homologue of the human Ran-binding protein, RanBP1, and has been renamed sbp1 (spi1-binding protein). sbp1p binds to spi1p-GTP and costimulates the GTPase-activating protein (GAP)-catalyzed GTPase activity. Cells in which sbp1p is depleted or overproduced phenocopy cells in which the balance between spi1p-GTP and spi1p-GDP is perturbed by other means. Therefore, sbp1p mediates and/or regulates the essential functions of the spi1p GTPase system. med2 and med3 encode novel fission yeast proteins that, based on our phenotypic analyses, are likely to identify additional regulators or effectors of the spi1p GTPase system.

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Bhatter, Nupur, Raju Roy, Shanaya Shah, Sneha P. Sastry, Sabnam Parbin, Rajan Iyappan, Siddharth Kankaria, and Purusharth I. Rajyaguru. "Arginine methylation augments Sbp1 function in translation repression and decapping." FEBS Journal 286, no. 23 (September 23, 2019): 4693–708. http://dx.doi.org/10.1111/febs.15057.

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Gong, Jianzhuang, and Li Li. "Sodium Selenite Inhibits Proliferation of Gastric Cancer Cells by Inducing SBP1 Expression." Tohoku Journal of Experimental Medicine 239, no. 4 (2016): 279–85. http://dx.doi.org/10.1620/tjem.239.279.

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Takano, Ryo, Hiroko Kozuka-Hata, Daisuke Kondoh, Hiroki Bochimoto, Masaaki Oyama, and Kentaro Kato. "A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes." iScience 19 (September 2019): 703–14. http://dx.doi.org/10.1016/j.isci.2019.07.035.

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Youngwilai, Atcharaporn, Pinit Kidkhunthod, Nichada Jearanaikoon, Jitrin Chaiprapa, Nontipa Supanchaiyamat, Andrew J. Hunt, Yuvarat Ngernyen, Thunyalux Ratpukdi, Eakalak Khan, and Sumana Siripattanakul-Ratpukdi. "Simultaneous manganese adsorption and biotransformation by Streptomyces violarus strain SBP1 cell-immobilized biochar." Science of The Total Environment 713 (April 2020): 136708. http://dx.doi.org/10.1016/j.scitotenv.2020.136708.

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Minamikawa, Mai F., Daisuke Fujii, Hiroyuki Kakui, Nobuhiro Kotoda, and Hidenori Sassa. "Identification of an S-RNase binding protein1 (SBP1) homolog of apple (Malus^|^times;domestica)." Plant Biotechnology 30, no. 2 (2013): 119–23. http://dx.doi.org/10.5511/plantbiotechnology.13.0109a.

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Rejwana, Iffat, Sadia Afrin Rimi, Shamima Sultana, and Sultana Ferdousi. "Cardiovascular responses to tilting in Type 2 Diabetic patients." Journal of Bangladesh Society of Physiologist 15, no. 2 (December 23, 2020): 78–84. http://dx.doi.org/10.3329/jbsp.v15i2.50922.

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Background: Diabetes mellitus (DM) is a disorder with a debilitating effects on cardiac autonomic control. Multiple major cardiovascular risk factors associated with DM led diabetic patients at high risk of Cardiovascular Disease. Objective: To assess cardiovascular responses to tilting in Type 2 Diabetic patients (T2DM) with normal and abnormal autonomic function test. Methods: This experimental study was conducted on 60 patients of T2DM. Among them, 30 patients were with normal cardiovascular reflex test (group DN) and 30 patients were with abnormal test (group DA). Thirty(30) apparently healthy subjects with similar age and sex without any physical illness were enrolled as control. Tilt table test of all subjects was done by tilting at 60° for 10 min by using a motorized tilt table. Cardiovascular response to tilt test was assessed by calculating D Heart rate (Acceleration index and Brake index); DSBP (SBP 30s-0 and SBP1 min -0), DDBP (DBP 30s-0 and DBP1 min -0) after tilting. For statistical analysis, one-way ANOVA followed by Bonferroni post hoc was used. Results: In this study, the Acceleration index was significantly higher in patient group DN compared to control and DA(p<0.001). But the Brake index was significantly (p<0.01, p<0.05) lower in both group of patients compared to control. In addition, SBP 30sec-0 and SBP1 min-0 were significantly higher in DA than those of control and DN.DBP 30sec-0 and DBP1min-0 were significantly (p<0.001) lower in DA patients compared to DN and control. Conclusion: This study concluded that cardiovascular response to tilting was weak in T2DM patients and it was greatly affected in T2DM patients with abnormal autonomic function test. J Bangladesh Soc Physiol. 2020, December; 15(2): 78-84

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Brandariz-Núñez, Alberto, Fuxing Zeng, Quan Ngoc Lam, and Hong Jin. "Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner." RNA 24, no. 1 (October 6, 2017): 43–55. http://dx.doi.org/10.1261/rna.062547.117.

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Qian, Yunyun, Yuhang Zhang, Yanfei Yu, Quan Li, Genglin Guo, Yang Fu, Huochun Yao, Chengping Lu, and Wei Zhang. "SBP1 is an adhesion-associated factor without the involvement of virulence in Streptococcus suis serotype 2." Microbial Pathogenesis 122 (September 2018): 90–97. http://dx.doi.org/10.1016/j.micpath.2018.06.008.

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Levray, Yvette S., Bianca Bana, Sarah J. Tarr, Emilia J. McLaughlin, Peter Rossi-Smith, Anita Waltho, Georgina H. Charlton, et al. "Formation of ER-lumenal intermediates during export of Plasmodium proteins containing transmembrane-like hydrophobic sequences." PLOS Pathogens 19, no. 3 (March 31, 2023): e1011281. http://dx.doi.org/10.1371/journal.ppat.1011281.

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During the blood stage of a malaria infection, malaria parasites export both soluble and membrane proteins into the erythrocytes in which they reside. Exported proteins are trafficked via the parasite endoplasmic reticulum and secretory pathway, before being exported across the parasitophorous vacuole membrane into the erythrocyte. Transport across the parasitophorous vacuole membrane requires protein unfolding, and in the case of membrane proteins, extraction from the parasite plasma membrane. We show that trafficking of the exported Plasmodium protein, Pf332, differs from that of canonical eukaryotic soluble-secreted and transmembrane proteins. Pf332 is initially ER-targeted by an internal hydrophobic sequence that unlike a signal peptide, is not proteolytically removed, and unlike a transmembrane segment, does not span the ER membrane. Rather, both termini of the hydrophobic sequence enter the ER-lumen and the ER-lumenal species is a productive intermediate for protein export. Furthermore, we show in intact cells, that two other exported membrane proteins, SBP1 and MAHRP2, assume a lumenal topology within the parasite secretory pathway. Although the addition of a C-terminal ER-retention sequence, recognised by the lumenal domain of the KDEL receptor, does not completely block export of SBP1 and MAHRP2, it does enhance their retention in the parasite ER. This indicates that a sub-population of each protein adopts an ER-lumenal state that is an intermediate in the export process. Overall, this suggests that although many exported proteins traverse the parasite secretory pathway as typical soluble or membrane proteins, some exported proteins that are ER-targeted by a transmembrane segment-like, internal, non-cleaved hydrophobic segment, do not integrate into the ER membrane, and form an ER-lumenal species that is a productive export intermediate. This represents a novel means, not seen in typical membrane proteins found in model systems, by which exported transmembrane-like proteins can be targeted and trafficked within the lumen of the secretory pathway.

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Crapanzano, Michael S., William B. Strong, Ingrid R. Newman, R. Lester Hixon, Devarra Casal, and Charles W. Linder. "Calf Blood Pressure: Clinical Implications and Correlations With Arm Blood Pressure in Infants and Young Children." Pediatrics 97, no. 2 (February 1, 1996): 220–24. http://dx.doi.org/10.1542/peds.97.2.220.

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Objective. Indirect measurement of lower extremity blood pressure is often used in the clinical setting, although normative data after the newborn period are not readily available. Methods. Indirect blood pressure (BP) measurement was obtained in the right arms and right calves of 148 healthy infants and young children 2 weeks to 3 years of age. All measurements were made using an oscillometric device. The infants and children were quiet or asleep and in the supine position. A BP cuff of proper size was chosen. Three measurements were made in both extremities; the average of the second and third measurements was used for all analyses. Results. Age correlated better with calf systolic blood pressure (SBPc) than with arm SBP (SBPa) (r = .52 vs .17). Calf diastolic blood pressure (DBPc) and calf mean blood pressure (MBPc) correlated moderately poorly with age (r = .37 and .39, respectively). There was no order effect. SBPc correlated best with height (r .53), then age (r = .52), and, finally, weight (r = .51). The correlation between BPc and BPa was moderately low. The correlation of SBPc with SBPa was r = .46; that of DBPc with DBPa was r = .37; and that of MBPc with MBPa was r = .41. From birth to 6 months, SBPc was slightly lower than SBPa (1 to 3 mm Hg). SBPc increased linearly relative to SBPa and began to exceed SBPa at 6 months of age. The pattern of DBP and MBP was similar. Wide variability of blood pressure parameters was noted between the infants and children at all ages. Conclusions. Reference data are presented for BPc and the difference between BPc and BPa in healthy infants and children from 2 weeks to 3 years of age. BPc is not equivalent to BPa and should not be arbitrarily substituted. Because of the wide variability among healthy infants and children, SBPc measurements should be interpreted with caution when evaluating for coarctation of the aorta.

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Nader, Joëlle S., Alice Boissard, Cécile Henry, Isabelle Valo, Véronique Verrièle, Marc Grégoire, Olivier Coqueret, Catherine Guette, and Daniel L. Pouliquen. "Cross-Species Proteomics Identifies CAPG and SBP1 as Crucial Invasiveness Biomarkers in Rat and Human Malignant Mesothelioma." Cancers 12, no. 9 (August 27, 2020): 2430. http://dx.doi.org/10.3390/cancers12092430.

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Malignant mesothelioma (MM) still represents a devastating disease that is often detected too late, while the current effect of therapies on patient outcomes remains unsatisfactory. Invasiveness biomarkers may contribute to improving early diagnosis, prognosis, and treatment for patients, a task that could benefit from the development of high-throughput proteomics. To limit potential sources of bias when identifying such biomarkers, we conducted cross-species proteomic analyzes on three different MM sources. Data were collected firstly from two human MM cell lines, secondly from rat MM tumors of increasing invasiveness grown in immunocompetent rats and human MM tumors grown in immunodeficient mice, and thirdly from paraffin-embedded sections of patient MM tumors of the epithelioid and sarcomatoid subtypes. Our investigations identified three major invasiveness biomarkers common to the three tumor sources, CAPG, FABP4, and LAMB2, and an additional set of 25 candidate biomarkers shared by rat and patient tumors. Comparing the data to proteomic analyzes of preneoplastic and neoplastic rat mesothelial cell lines revealed the additional role of SBP1 in the carcinogenic process. These observations could provide new opportunities to identify highly vulnerable MM patients with poor survival outcomes, thereby improving the success of current and future therapeutic strategies.

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O’Brien, Martin, Geneviève Major, Sier-Ching Chantha, and Daniel P. Matton. "Isolation of S-RNase binding proteins from Solanum chacoense: identification of an SBP1 (RING finger protein) orthologue." Sexual Plant Reproduction 17, no. 2 (May 19, 2004): 81–87. http://dx.doi.org/10.1007/s00497-004-0218-8.

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Fang, Wenfeng, Marci L. Goldberg, Nicole M. Pohl, Xiuli Bi, Chang Tong, Bin Xiong, Timothy J. Koh, Alan M. Diamond, and Wancai Yang. "Functional and physical interaction between the selenium-binding protein 1 (SBP1) and the glutathione peroxidase 1 selenoprotein." Carcinogenesis 31, no. 8 (June 7, 2010): 1360–66. http://dx.doi.org/10.1093/carcin/bgq114.

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Dervisi, Irene, Chrysanthi Valassakis, Adamantia Agalou, Nikolaos Papandreou, Varvara Podia, Kosmas Haralampidis, Vassiliki A. Iconomidou, Vassili N. Kouvelis, Herman P. Spaink, and Andreas Roussis. "Investigation of the interaction of DAD1-LIKE LIPASE 3 (DALL3) with Selenium Binding Protein 1 (SBP1) in Arabidopsis thaliana." Plant Science 291 (February 2020): 110357. http://dx.doi.org/10.1016/j.plantsci.2019.110357.

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Dervisi, Irene, Kosmas Haralampidis, and Andreas Roussis. "Investigation of the interaction of a papain-like cysteine protease (RD19c) with selenium-binding protein 1 (SBP1) in Arabidopsis thaliana." Plant Science 315 (February 2022): 111157. http://dx.doi.org/10.1016/j.plantsci.2021.111157.

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Iriko, Hideyuki, Tomoko Ishino, Mayumi Tachibana, Ayaka Omoda, Motomi Torii, and Takafumi Tsuboi. "Skeleton binding protein 1 (SBP1) of Plasmodium falciparum accumulates in electron-dense material before passing through the parasitophorous vacuole membrane." Parasitology International 75 (April 2020): 102003. http://dx.doi.org/10.1016/j.parint.2019.102003.

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Fujita, H. "Mammalian class E Vps proteins, SBP1 and mVps2/CHMP2A, interact with and regulate the function of an AAA-ATPase SKD1/Vps4B." Journal of Cell Science 117, no. 14 (June 15, 2004): 2997–3009. http://dx.doi.org/10.1242/jcs.01170.

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Dutilleul, Christelle, Agnès Jourdain, Jacques Bourguignon, and Véronique Hugouvieux. "The Arabidopsis Putative Selenium-Binding Protein Family: Expression Study and Characterization of SBP1 as a Potential New Player in Cadmium Detoxification Processes." Plant Physiology 147, no. 1 (March 19, 2008): 239–51. http://dx.doi.org/10.1104/pp.107.114033.

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32

Nurazizah, Siti, Setyo Budi, and Wiharyanti Nur Lailiyah. "PERTUMBUHAN BERBAGAI KLON TANAMAN TEBU (Saccharum officinarum L.) DI KEBUN JUWET DUKUHDIMORO, MOJOAGUNG – JOMBANG." Agroplantae: Jurnal Ilmiah Terapan Budidaya dan Pengelolaan Tanaman Pertanian dan Perkebunan 11, no. 2 (September 22, 2022): 87–100. http://dx.doi.org/10.51978/agro.v11i2.463.

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Produktivitas gula tahun 2015-2019 mengalami penurunan, karena varietas yang digunakan mengalami degradasi genetik mengakibatkan pertumbuhan maupun produktivitasnya menurun. Tujuan penelitian untuk mengetahui keragaman genetic dan pertumbuhan tanaman tebu (Saccharum officinarum L.) pada klon SB01, SB03, SB04, SB11, SB12, SB19, dan SB20. Penelitian dilakukan di kebun Pusat Penelitian dan Pengembangan Tanaman Tebu (P3T) PG Gempolkrep PT Perkebunan Nusantara X (PTPN X) Desa Dukuhdimoro, Kecamatan Mojoagung, Kabupaten Jombang pada bulan Februari – April 2022. Bahan yang diuji adalah Klon SB01, SB03, SB04, SB11, SB12, SB19, SB20, Varietas BL dan Varietas PS881. Variabel yang diamati terdiri dari pertambahan tinggi batang, pertambahan diameter batang, jumlah batang, jumlah daun, dan luas daun. Analisis data menggunakan ANOVA 5%, uji BNT, keragaman genetik, heritabilitas dan kemajuan genetik. Hasil penelitian menunjukan Klon SB 12 memiliki pertumbuhan terbaik diantaranya pertambahan tinggi batang 103,80; jumlah batang 4,17 (MST) dan 5,00 (33 MST); luas daun 646,08 cm2 (31 MST) dan 52, 67 cm2 (33 MST). Nilai Heritabilitas karakter pertambahan tinggi batang (129,08) dan luas daun (1546,79). Nilai kemajuan genetik (239,85) dan luas daun (3035,64). Maka, seleksi pada karakter pertumbuhan tersebut efektif.

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Sood, Sandeep, Sirisha Anne, Kuldeep Kumar Ashta, and Ravi Kumar. "Comparison of ambulatory blood pressure monitoring and self-blood pressure monitoring for diagnosing white coat hypertension amongst pregnant women." International Journal of Reproduction, Contraception, Obstetrics and Gynecology 9, no. 1 (December 26, 2019): 274. http://dx.doi.org/10.18203/2320-1770.ijrcog20196033.

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Background: White coat hypertension (WCH) is a common and well recognized phenomenon. It is also very prevalent amongst pregnant women and is often diagnosed as chronic/ gestational hypertension leading to unnecessary medications during pregnancy. ABPM is the gold standard for diagnosis of WCH. SBPM is an easy effective and reliable method to measure blood pressure but its efficacy needs to be tested and compared with ABPM in cases of WCH. It is important to compare the two methods in assessing WCH so SBPM can be utilized in cases of WCH, if found useful and efficacious.Methods: All pregnant women who presented to the ANC were screened for hypertension. Those who were diagnosed to be hypertensive in antenatal clinic and these patients were then admitted for ambulatory blood pressure monitoring (ABPM) for 24 hours and SBPM on 6 hourly bases for 5 days.Results: The ABPM and SBPB readings were noted, tabulated and compared. It was found that the prevalence of ‘WCH’ in this study using ABPM and SBPM were 47.368% (27/54) and 45.614% (26/54) respectively.Conclusions: The results in diagnosing WCH using ABPM and SBPM were comparable.

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Kemper, C., P. F. Zipfel, and I. Gigli. "The complement cofactor protein (SBP1) from the barred sand bass (Paralabrax nebulifer) mediates overlapping regulatory activities of both human C4BP and factor H." Molecular Immunology 35, no. 6-7 (April 1998): 351. http://dx.doi.org/10.1016/s0161-5890(98)90613-7.

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Dervisi, Irene, Orfeas Petropoulos, Adamantia Agalou, Varvara Podia, Nikolaos Papandreou, Vassiliki A. Iconomidou, Kosmas Haralampidis, and Andreas Roussis. "The SAH7 Homologue of the Allergen Ole e 1 Interacts with the Putative Stress Sensor SBP1 (Selenium-Binding Protein 1) in Arabidopsis thaliana." International Journal of Molecular Sciences 24, no. 4 (February 10, 2023): 3580. http://dx.doi.org/10.3390/ijms24043580.

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In this study, we focused on a member of the Ole e 1 domain-containing family, AtSAH7, in Arabidopsis thaliana. Our lab reports for the first time on this protein, AtSAH7, that was found to interact with Selenium-binding protein 1 (AtSBP1). We studied by GUS assisted promoter deletion analysis the expression pattern of AtSAH7 and determined that the sequence 1420 bp upstream of the transcription start can act as a minimal promoter inducing expression in vasculature tissues. Moreover, mRNA levels of AtSAH7 were acutely increased under selenite treatment in response to oxidative stress. We confirmed the aforementioned interaction in vivo, in silico and in planta. Following a bimolecular fluorescent complementation approach, we determined that the subcellular localization of the AtSAH7 and the AtSAH7/AtSBP1 interaction occur in the ER. Our results indicate the participation of AtSAH7 in a biochemical network regulated by selenite, possibly associated with responses to ROS production.

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Mutso, Amelia A., Bogdan Petre, Lejian Huang, Marwan N. Baliki, Souraya Torbey, Kristina M. Herrmann, Thomas J. Schnitzer, and A. Vania Apkarian. "Reorganization of hippocampal functional connectivity with transition to chronic back pain." Journal of Neurophysiology 111, no. 5 (March 1, 2014): 1065–76. http://dx.doi.org/10.1152/jn.00611.2013.

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The hippocampus has been shown to undergo significant changes in rodent models of neuropathic pain; however, the role of the hippocampus in human chronic pain and its contribution to pain chronification have remained unexplored. Here we examine hippocampal processing during a simple visual attention task. We used functional MRI to identify intrinsic and extrinsic hippocampal functional connectivity (synchronous neural activity), comparing subacute back pain (SBP, back pain 1–4 mo) and chronic back pain (CBP, back pain >10 yr) patients to control (CON) subjects. Both groups showed more extensive hippocampal connectivity than CON subjects. We then examined the evolution of hippocampal connectivity longitudinally in SBP patients who recovered (SBPr, back pain decreased >20% in 1 yr) and those with persistent pain (SBPp). We found that SBPp and SBPr subjects have distinct changes in hippocampal-cortical connectivity over 1 yr; specifically, SBPp subjects showed large decreases in hippocampal connectivity with medial prefrontal cortex (HG-mPFC). Furthermore, in SBP patients the strength of HG-mPFC reflected variations in back pain over the year. These relationships were replicated when examined in a different task performed by SBP patients (rating fluctuations of back pain), indicating that functional connectivity of the hippocampus changes robustly in subacute pain and the nature of these changes depends on whether or not patients recover from SBP. The observed reorganization of processing within the hippocampus and between the hippocampus and the cortex seems to contribute to the transition from subacute to chronic pain and may also underlie learning and emotional abnormalities associated with chronic pain.

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Fasheh, Miftah Farid, Setyo Budi, and Wiharyanti Murlailiyah. "Study Of Growth and Production Of Seven Clones Of Sugarcane (Saccharum officinarum L.) Inalluvial Soilin Sambiroto Village, Sooko District-Mojokerto." Nabatia 10, no. 2 (December 31, 2022): 57–65. http://dx.doi.org/10.21070/nabatia.v10i2.1611.

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The productivity of sugarcane in Indonesia is still decreasing due to the low value of crystal produced per hectare. One of the main and fundamental factors is the limited availability of superior varieties. The purpose of this study was to determine the differences in growth andyield of clones SB01, SB03 clones, SB04 clones, SB11 clones, SB12 clones, SB19 clones andSB20 clones as well as sugarcane clones that had the best growth. This study used a randomized block design with one factor, namely sugarcane clones SB01, SB3 clones, SB04 clones, SB011 clones, SB12 clones, SB19 clones, SB20 clones. Each replication consisted of 7 sugarcane clones. Which was repeated three times with observation variables which included growth andyield variables. The data were then analyzed using ANOVA variance. If there is a significant difference, continue with the 5% BNT test. There were significant differences between seven sugarcane clones in growth andyield variables in sugarcane. Of the seven clones observed, clone SB12 had good vegetative andgenerative potential with the highestyield of160.67 tons/ha, clone SB01 had the highestyield potential, 11.3% and clone SB01 had the highest crystallizationpotential. namely with a value of 17.6 tons/ha.

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Irawan, Kristia Andre, Budi Setyo, and Suhaili Suhaili. "Keragaman Morfologi Pertumbuhan 7 Klon dan 2 Varietas Tanaman Tebu (Saccharum officinarum L.) di PT Perkebunan Nusantara X Ploso Klaten-Kediri." Gema Agro 28, no. 1 (April 27, 2023): 42–51. http://dx.doi.org/10.22225/ga.28.1.6634.42-51.

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Sugarcane (Saccharum Officinarum L.) is the basic ingredient for making sugar. In Indonesia, in the last 5 years, sugar productivity only reached 5.2 tons/ha, very different from the productivity in 1935-1940, which averaged 17 tons/ha. This is because this Aat variety has not yet reached the desired productivity rate. The purpose of this study was to determine the diversity of growth of sugarcane (Saccharum officinarum L.) in clone SB01, clone SB03, clone SB04, clone SB11, clone SB12, clone SB19, clone SB20, variety PS862 and variety Bululawang. This research was carried out in the Djengkol garden, Ploso Klaten, Kediri. The tools used are sickle, cloth, tape measure, caliper, camera and stationery. The materials used are clone SB01, clone SB03, SB11, SB12, SB19, SB20, PS862 variety, Bululawang variety. Observations included growth variables (stem height, number of stems, number of leaves, stem diameter). Data analysis used ANOVA with a 5% F test. If there is a significant difference, proceed with the 5% DMRT test, correlation test, genetic diversity, heritability, genetic progress. Clone SB12 had an advantage in variable stem height of 395.83 (cm), number of stems 5.67 (stem), number of leaves 9.44 (strands), while clone SB01 had an advantage in stem diameter with the highest value of 31.99 (mm). Correlation relationship on sugarcane plant growth variables. The diversity of 7 clones and 2 varieties is heavily influenced by genetics and slightly influenced by the environment as shown by the KKG values in the medium-high category, the KF values in the low-high category, the Heritability (H2) medium-high category and the genetic progress (KG) category. rather high.

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Kemper, Claudia, Peter F. Zipfel, and Irma Gigli. "The Complement Cofactor Protein (SBP1) from the Barred Sand Bass (Paralabrax nebulifer) Mediates Overlapping Regulatory Activities of Both Human C4b Binding Protein and Factor H." Journal of Biological Chemistry 273, no. 31 (July 31, 1998): 19398–404. http://dx.doi.org/10.1074/jbc.273.31.19398.

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Sachan, Richa, Amit Kundu, Prasanta Dey, Ji Yeon Son, Kyeong Seok Kim, Da Eun Lee, Hae Ri Kim, et al. "Dendropanax morbifera Protects against Renal Fibrosis in Streptozotocin-Induced Diabetic Rats." Antioxidants 9, no. 1 (January 19, 2020): 84. http://dx.doi.org/10.3390/antiox9010084.

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The aquatic extract of Dendropanax morbifera (DP) is typically consumed as a beverage in Korea and China and is also used in various traditional medicines. However, the functional role of DP on diabetes-induced renal fibrosis is unclear. Here, the protective effects of DP extract against diabetes-induced renal fibrosis were evaluated. Streptozotocin (STZ, 60 mg/kg) was injected intraperitoneally in rats to induce diabetes. After 5 days, DP extract (25 mg/kg/day) and metformin (50 mg/kg/day) were administered orally to diabetic rats for 28 days. DP administration protected both body and organ weight loss in STZ-treated diabetic rats. Significant improvements in serum blood urea nitrogen (BUN), creatinine, and oxidative stress parameters were observed in diabetic rats by DP administration. DP extract markedly protected diabetic-induced histopathological damages in the kidney and pancreas. A significant reduction was observed in microalbumin, kidney injury molecule-1 (KIM-1), selenium binding protein-1 (SBP1), and pyruvate kinase muscle isozyme M2 (PKM2) levels in the urinary excretion of diabetic rats after the administration of DP extract. The expression of pro-inflammatory cytokines and fibrosis marker levels were significantly reduced in the kidney of diabetic rats. Our results strongly indicate that DP extract exhibits protective activity against diabetes-induced renal fibrosis through ameliorating oxidative stress and inflammation. Therefore, we suggest that DP extract can be used as a preventive agent on the progression of diabetic nephropathy and renal fibrosis.

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Dey, Prasanta, Amit Kundu, Ha Eun Lee, Babli Kar, Vineet Vishal, Suvakanta Dash, In Su Kim, Tejendra Bhakta, and Hyung Sik Kim. "Molineria recurvata Ameliorates Streptozotocin-Induced Diabetic Nephropathy through Antioxidant and Anti-Inflammatory Pathways." Molecules 27, no. 15 (August 5, 2022): 4985. http://dx.doi.org/10.3390/molecules27154985.

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Molineria recurvata (MR) has been traditionally used to manage diabetes mellitus in India. However, the molecular mechanism of MR on the diabetic-induced nephropathy has not been clearly investigated. Thus, this study investigates the protective effects of the MR extract on nephropathy in streptozotocin (STZ)-induced diabetic rats. Diabetes was instigated by a single intraperitoneal injection of STZ (45 mg/kg) in male Sprague-Dawley rats. Once the diabetes was successfully induced, the MR extract (200 mg/kg/day) or metformin (200 mg/kg/day) was orally administered for 14 days. Renal function, morphology changes and levels of inflammatory cytokines were measured. Blood glucose concentrations were considerably reduced in STZ-induced diabetic rats following treatment with the MR extract. The administration of the MR extract substantially restored the abnormal quantity of the oxidative DNA damage marker 8-hydroxy-2′-deoxy-guanosine (8-OHdG), malondialdehyde, glutathione, oxidized glutathione, superoxide dismutase, catalase, interleukin (IL)-1β, IL-6, IL-10, and transforming growth factor-β (TGF-β). The urinary excretion of kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), selenium binding protein 1 (SBP1), and pyruvate kinase M2 (PKM2) was significantly reduced in diabetes rats after administration of the MR extracts. In the kidneys of STZ-induced diabetic rats, the MR extracts markedly downregulated the expression of fibronectin, collagen-1, and α-smooth muscle actin (α-SMA). In particular, the MR extracts markedly increased the level of SIRT1 and SIRT3 and reduced claudin-1 in the kidney. These results suggest that the MR extracts exhibits therapeutic activity in contrast to renal injury in STZ-induced diabetic rats through repressing inflammation and oxidative stress.

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Oliva, Karen, Gillian Barker, Gregory E. Rice, Mark J. Bailey, and Martha Lappas. "2D-DIGE to identify proteins associated with gestational diabetes in omental adipose tissue." Journal of Endocrinology 218, no. 2 (May 24, 2013): 165–78. http://dx.doi.org/10.1530/joe-13-0010.

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Gestational diabetes mellitus (GDM) is a significant risk factor for the type 2 diabetes epidemic in many populations. Maternal adipose tissue plays a central role in the pathophysiology of GDM. Thus, the aim of this study was to determine the effect of GDM on the proteome of adipose tissue. Omental adipose tissue was obtained at the time of term Caesarean section from women with normal glucose tolerance (NGT) or GDM. 2D-difference gel electrophoresis (DIGE), followed by mass spectrometry, was used to identify protein spots (n=6 patients per group). Western blotting was used for confirmation of six of the spot differences (n=6 patients per group). We found 14 proteins that were differentially expressed between NGT and GDM adipose tissue (≥1.4-fold,P<0.05). GDM was associated with an up-regulation of four proteins: collagen alpha-2(VI) chain (CO6A2 (COL6A2)), fibrinogen beta chain (FIBB (FGB)), lumican (LUM) and S100A9. On the other hand, a total of ten proteins were found to be down-regulated in adipose tissue from GDM women. These were alpha-1-antitrypsin (AIAT (SERPINA1)), annexin A5 (ANXA5), fatty acid-binding protein, adipocyte (FABP4), glutathione S-transferase P (GSTP (GSTP1)), heat-shock protein beta-1 (HSP27 (HSPB1)), lactate dehydrogenase B chain (LDHB), perilipin-1 (PLIN1), peroxiredoxin-6 (PRX6 (PRDX6)), selenium-binding protein 1 (SBP1) and vinculin (VINC (VCL)). In conclusion, proteomic analysis of omental fat reveals differential expression of several proteins in GDM patients and NGT pregnant women. This study revealed differences in expression of proteins that are involved in inflammation, lipid and glucose metabolism and oxidative stress and added further evidence to support the role of visceral adiposity in the pathogenesis of GDM.

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Kovalenko, Ye L., and O. K. Melekhovets. "INTRAVENOUS LASER THERAPY IN A COMPREHENSIVE APPROACH TO THE CORRECTION OF RISK FACTORS OF ARTERIAL HYPERTENSION." Eastern Ukrainian Medical Journal 8, no. 1 (2020): 49–51. http://dx.doi.org/10.21272/eumj.2020;8(1):43-51.

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Introduction. According to Akl C et al. by 2025, the number of people with arterial hypertension (AH) will increase by 15–20% and reach 1.5 billion people. Since hyperuricemia (HU) is closely related to other AH risk factors, there is a need to study the relationship between HU and other AH risk factors. Objective of this work is to develop rational approaches to modifying individual AH risk factor using intravenous laser therapy (IVLT). Materials and methods. The study included 184 people: Group 1 (n = 30) – normotensive individuals without HU; Group 2 (n = 52) – normotensive patients with HU; Group 3 (n = 48) – patients with essential AH (stage I, 1-2 degree) without HU; Group 4 (n = 54) – patients with essential AH (stage I, 1-2 degree) with HU. Patients in Group 3 and 4 were divided into subgroups according to the treatment regimens: 3A (n = 24), 4A (n = 26) (standard antihypertensive therapy (AHT)) and 3B (n = 24), 4B (n = 28) (combination treatment with AHT and IVLT). The IVLT course was performed with a wavelength of 635 nm, a power of 1.5 mW, a radiation power density of 0.2 W/cm2, a fluence of 0.2 J/cm2, an exposure of 900 seconds, the course – daily, with a total of 10 procedures. Study results. The association between the level of uric acid (UA), systolic blood pressure (SBP), diastolic blood pressure (DBP), endothelial dysfunction (ED), left ventricular myocardial dysfunction, excess increase in arterial wall stiffness, and poikilocytosis in the study groups was established. The use of IVLT in combination with AHT allows to achieve a statistically significant (р < 0.05), compared to AHT reduction in SBPd by 4.2%, DBPd by 2.4%, DBPn by 2.5%, time index (TI) SBPd by 5.1%, TI DBPd by 2.7%, TI SBPn by 19%, rate of morning rise (RMR) SBP by 33.8%, RMR DBP by 31%, early morning blood pressure surge (EMBPS) SBP by 17.3%, EMBPS DBP by 12.8%, puilse wave velosity (PWV) by 4.1%, manifestations of endothelial dysfunction by 1.4%, myocardial dysfunction by 4.5%, poikilocytosis by 2.9%, uric acid level by 3.1% in patients with AH. In AH and HU comorbidity, addition of ILT to AHT allows to achieve an additional reduction in SBPd by 9.3%, DBPd by 7.4%, SBPn by 11,5%, DBPn by 2.7%, TI SBPd by 18.8%, TI DBPd by 18.9%, TI SBPn by 1.8%, TI DBPn by 8,7%, RMR SBP by 25.8%, RMR DBP by 28.5%, EMBPS SBP by 8.2%, EMBPS DBP by 6.0%, PWV by 13.4%, endothelial dysfunction by 3.5%, myocardial dysfunction by 18.8%, poikilocytosis by 5.7%, uric acid level by 11.6% compared to AHT. In patients with normal blood pressure and HU values, the use of IVLT can reduce DBPM, EDVD, poikilocytosis, and UA level parameters (p < 0.05). Conclusions. The presence of direct correlations of average strength between HU and endothelial dysfunction, systolic diastolic dysfunction, excessive increase in arterial wall stiffness, and poikilocytosis was found. The use of IVLT in normotensive and hypertensive patients with AH with an effective method of UA level correction, excessive arterial wall stiffness, myocardial dysfunction, ED and poikilocytosis.

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Dahmen, A., T. Kaidoh, P. F. Zipfel, and I. Gigli. "Cloning and characterization of a cDNA representing a putative complement-regulatory plasma protein from barred sand bass (Parablax neblifer)." Biochemical Journal 301, no. 2 (July 15, 1994): 391–97. http://dx.doi.org/10.1042/bj3010391.

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It has been demonstrated previously that plasma from a number of vertebrate species including the phylogenetically old barred sand bass possesses molecules that cleave the alpha'-chain of the activated third (C3b) and fourth (C4b) components of the human complement system. A specific protease and a cofactor protein were identified to be responsible for this cleavage. The cofactor activity in sand bass correlated with a 110 kDa polypeptide chain of a 360 kDa plasma protein. The evolutionary conservation was probed at the cDNA level and subsequently a cDNA clone of barred sand bass was isolated that represents a protein with structural similarity to mammalian complement-regulatory proteins. The cDNA (SB1) was identified by immunoscreening of a sand bass liver expression library using affinity-purified IgG antibodies raised against the isolated 110 kDa material. The cDNA is 3397 bp in size and the open reading frame represents a protein of 1053 amino acid residues with a hydrophobic signal peptide indicative of a secreted protein. The calculated mass of the mature protein (SBP1) is 115.2 kDa which is in good agreement with the molecular mass of 110 kDa determined for the sand bass serum protein. Similarly to mammalian complement-regulatory proteins, the protein deduced from the sand bass cDNA is organized into short consensus repeats (SCR). It consists of 17 SCRs, of which SCRs 2, 12 and 16 exhibit significant homology to SCRs 2, 15 and 19 of human factor H, and SCRs 11, 12 and 13 have homology to SCRs 1, 2 and 3 of human C4b-binding protein. For the first time a complete cDNA representing a putative complement-regulatory protein which is structurally related to mammalian complement proteins has been isolated from a bony fish.

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Lin, Yezhe, Ivan De Araujo, Gelsina Stanley, Dana Small, and Paul Geha. "Chronic pain precedes disrupted eating behavior in low-back pain patients." PLOS ONE 17, no. 2 (February 10, 2022): e0263527. http://dx.doi.org/10.1371/journal.pone.0263527.

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Chronic pain is associated with anhedonia and decreased motivation. These behavioral alterations have been linked to alterations in the limbic brain and could explain the increased risk for obesity in pain patients. The mechanism of these behavioral changes and how they set in in relation to the development of chronic pain remain however poorly understood. Here we asked how eating behavior was affected in low-back pain patients before and after they transitioned to chronic pain, compared to patients whose pain subsided. Additionally, we assessed how the hedonic perception of fat-rich food, which is altered in chronic pain patients, related to the properties of the nucleus accumbens in this patients’ population. We hypothesized that the accumbens would be directly implicated in the hedonic processing of fat-rich food in pain patients because of its well-established role in hedonic feeding and fat ingestion, and its emerging role in chronic pain. Accordingly, we used behavioral assays and structural brain imaging to test sub-acute back pain patients (SBP) and healthy control subjects at baseline and at approximately one-year follow-up. We also studied a sample of chronic low-back pain patients (CLBP) at one time point only. We found that SBP patients who recovered at follow-up (SBPr) and CLBP patients showed disrupted eating behaviors. In contrast, SBP patients who persisted in having pain at follow-up (SBPp) showed intact eating behavior. From a neurological standpoint, only SBPp and CLBP patients showed a strong and direct relationship between hedonic perception of fat-rich food and nucleus accumbens volume. This suggests that accumbens alterations observed in SBPp patients in previous works might protect them from hedonic eating disruptions during the early course of the illness. We conclude that disrupted eating behavior specifically sets in after pain chronification and is accompanied by structural changes in the nucleus accumbens.

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Mosqueda, Juan, Diego Josimar Hernandez-Silva, Massaro W. Ueti, Adolfo Cruz-Reséndiz, Ricardo Marquez-Cervantez, Uriel Mauricio Valdez-Espinoza, Minh-Anh Dang-Trinh, et al. "Spherical Body Protein 4 from Babesia bigemina: A Novel Gene That Contains Conserved B-Cell Epitopes and Induces Cross-Reactive Neutralizing Antibodies in Babesia ovata." Pathogens 12, no. 3 (March 22, 2023): 495. http://dx.doi.org/10.3390/pathogens12030495.

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Bovine babesiosis is a tick-transmitted disease caused by intraerythrocytic protozoan parasites of the genus Babesia. Its main causative agents in the Americas are Babesia bigemina and Babesia bovis, while Babesia ovata affects cattle in Asia. All Babesia species secrete proteins stored in organelles of the apical complex, which are involved in all steps of the invasion process of vertebrate host cells. Unlike other apicomplexans, which have dense granules, babesia parasites instead have large, round intracellular organelles called spherical bodies. Evidence suggests that proteins from these organelles are released during the process of invading red blood cells, where spherical body proteins (SBPs) play an important role in cytoskeleton reorganization. In this study, we characterized the gene that encodes SBP4 in B. bigemina. This gene is transcribed and expressed in the erythrocytic stages of B. bigemina. The sbp4 gene consists of 834 nucleotides without introns that encode a protein of 277 amino acids. In silico analysis predicted a signal peptide that is cleaved at residue 20, producing a 28.88-kDa protein. The presence of a signal peptide and the absence of transmembrane domains suggest that this protein is secreted. Importantly, when cattle were immunized with recombinant B. bigemina SBP4, antibodies identified B. bigemina and B. ovata merozoites according to confocal microscopy observations and were able to neutralize parasite multiplication in vitro for both species. Four peptides with predicted B-cell epitopes were identified to be conserved in 17 different isolates from six countries. Compared with the pre-immunization sera, antibodies against these conserved peptides reduced parasite invasion in vitro by 57%, 44%, 42%, and 38% for peptides 1, 2, 3, and 4, respectively (p < 0.05). Moreover, sera from cattle infected with B. bigemina cattle contained antibodies that recognized the individual peptides. All these results support the concept of spb4 as a new gene in B. bigemina that should be considered a candidate for a vaccine to control bovine babesiosis.

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47

Freire-Maia, Ademar. "Carolina SBPC Bori." Psicologia USP 9, no. 1 (1998): 189–90. http://dx.doi.org/10.1590/s0103-65641998000100037.

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J.E.H. "SBPH Annual Meeting." Americas 42, no. 1 (July 1985): 103. http://dx.doi.org/10.1017/s0003161500015728.

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Vogt, Carlos, Ildeu de Castro Moreira, and Vanderlan Bolzani. "SBPC 70 anos." Ciência e Cultura 70, no. 3 (July 2018): 26–27. http://dx.doi.org/10.21800/2317-66602018000300008.

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Wei, Wei, Junichi Inaba, Yan Zhao, Joseph D. Mowery, and Rosemarie Hammond. "Phytoplasma Infection Blocks Starch Breakdown and Triggers Chloroplast Degradation, Leading to Premature Leaf Senescence, Sucrose Reallocation, and Spatiotemporal Redistribution of Phytohormones." International Journal of Molecular Sciences 23, no. 3 (February 5, 2022): 1810. http://dx.doi.org/10.3390/ijms23031810.

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Witches’-broom (WB, excessive initiation, and outgrowth of axillary buds) is one of the remarkable symptoms in plants caused by phytoplasmas, minute wall-less intracellular bacteria. In healthy plants, axillary bud initiation and outgrowth are regulated by an intricate interplay of nutrients (such as sugars), hormones, and environmental factors. However, how these factors are involved in the induction of WB by phytoplasma is poorly understood. We postulated that the WB symptom is a manifestation of the pathologically induced redistribution of sugar and phytohormones. Employing potato purple top phytoplasma and its alternative host tomato (Solanum lycopersicum), sugar metabolism and transportation, and the spatiotemporal distribution of phytohormones were investigated. A transmission electron microscopy (TEM) analysis revealed that starch breakdown was inhibited, resulting in the degradation of damaged chloroplasts, and in turn, premature leaf senescence. In the infected source leaves, two marker genes encoding asparagine synthetase (Sl-ASN) and trehalose-6-phosphate synthase (Sl-TPS) that induce early leaf senescence were significantly up-regulated. However, the key gibberellin biosynthesis gene that encodes ent-kaurene synthase (Sl-KS) was suppressed. The assessment of sugar content in various infected tissues (mature leaves, stems, roots, and leaf axils) indicated that sucrose transportation through phloem was impeded, leading to sucrose reallocation into the leaf axils. Excessive callose deposition and the resulting reduction in sieve pore size revealed by aniline blue staining and TEM provided additional evidence to support impaired sugar transport. In addition, a spatiotemporal distribution study of cytokinin and auxin using reporter lines detected a cytokinin signal in leaf axils where the axillary buds initiated. However, the auxin responsive signal was rarely present in such leaf axils, but at the tips of the newly elongated buds. These results suggested that redistributed sucrose as well as cytokinin in leaf axils triggered the axillary bud initiation, and auxin played a role in the bud elongation. The expression profiles of genes encoding squamosa promoter-binding proteins (Sl-SBP1), and BRANCHED1 (Sl-BRC1a and Sl-BRC1b) that control axillary bud release, as determined by quantitative reverse transcription (qRT)-PCR, indicated their roles in WB induction. However, their interactions with sugars and cytokinins require further study. Our findings provide a comprehensive insight into the mechanisms by which phytoplasmas induce WB along with leaf chlorosis, little leaf, and stunted growth.

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Journal articles on the topic 'Sbp1'

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