Journal articles on the topic 'Sanguinins'
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1
Chomienne, C., P. Mboungou, C. Dosquet, J. D. Rain, and Y. Najean. "Utilisation des méthodes isotopiques. Étude des volumes sanguins et des cellules sanguines circulantes." EMC - Hématologie 5, no. 3 (January 2010): 1–7. http://dx.doi.org/10.1016/s1155-1984(10)49941-0.
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Tu, Yan, Yadong Chen, Chongyang Zheng, and Hui Chen. "Platelet aggregation promoted by biofilms of oral bacteria and the effect of mouth rinses in vitro." Journal of Infection in Developing Countries 10, no. 07 (August 2, 2016): 704–11. http://dx.doi.org/10.3855/jidc.6639.
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Introduction: The purpose of this study was to observe platelet aggregation promoted by biofilms of Streptococcus sanguinis and Porphyromonas gingivalis and to evaluate the effect of two different mouth rinses on this process. Methodology: In the first experiment, the same amount of S. sanguinis, P. gingivalis, and the S. sanguinis + P. gingivalis mixed solution was added to an equivalent amount of platelet-rich plasma (PRP). Aggregation was measured using a recording platelet aggregometer. In the second experiment, S. sanguinis, P. gingivalis, S sanguinis + P. gingivalis mixed solutions were pretreated with either Listerine antiseptic mouth rinse or Xipayi mouth rinse for 3 minutes, 6 minutes, and 10 minutes, respectively. The same amount of solution was added to the PRP, and the inhibition of aggregation was measured. Results: In the first experiment, S. sanguinis and P. gingivalis were able to induce platelet aggregation. The aggregation rate of S. sanguinis + P. gingivalis was significantly lower than that of either S. sanguinis or P. gingivalis. In the second experiment, when S. sanguinis, P. gingivalis, and the S. sanguinis + P. gingivalis mixed solutions were pretreated with Listerine antiseptic mouth rinse for 3 minutes and Xipayi mouth rinse for 10 minutes, there was no significant platelet aggregation. Conclusions: Platelets could adhere to S. sanguinis or P. gingivalis, but when S. sanguinis was mixed with P. gingivalis, the aggregation rate was reduced significantly. Treatment with Listerine antiseptic mouth rinse or Xipayi mouth rinse inhibited the ability of the bacteria to induce platelet aggregation.
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Caufield, Page W., Ananda P. Dasanayake, Yihong Li, Yaping Pan, Jay Hsu, and J. Michael Hardin. "Natural History of Streptococcus sanguinis in the Oral Cavity of Infants: Evidence for a Discrete Window of Infectivity." Infection and Immunity 68, no. 7 (July 1, 2000): 4018–23. http://dx.doi.org/10.1128/iai.68.7.4018-4023.2000.
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ABSTRACT The heterogeneous group of oral bacteria within the sanguinis (sanguis) streptococci comprise members of the indigenous biota of the human oral cavity. While the association of Streptococcus sanguinis with bacterial endocarditis is well described in the literature, S. sanguinis is thought to play a benign, if not a beneficial, role in the oral cavity. Little is known, however, about the natural history of S. sanguinis and its specific relationship with other oral bacteria. As part of a longitudinal study concerning the transmission and acquisition of oral bacteria within mother-infant pairs, we examined the initial acquisition of S. sanguinis and described its colonization relative to tooth emergence and its proportions in plaque and saliva as a function of other biological events, including subsequent colonization with mutans streptococci. A second cohort of infants was recruited to define the taxonomic affiliation of S. sanguinis. We found that the colonization of the S. sanguinis occurs during a discrete “window of infectivity” at a median age of 9 months in the infants. Its colonization is tooth dependent and correlated to the time of tooth emergence; its proportions in saliva increase as new teeth emerge. In addition, early colonization of S. sanguinis and its elevated levels in the oral cavity were correlated to a significant delay in the colonization of mutans streptococci. Underpinning this apparent antagonism between S. sanguinis and mutans streptococci is the observation that after mutans streptococci colonize the infant, the levels of S. sanguinis decrease. Children who do not harbor detectable levels of mutans streptococci have significantly higher levels of S. sanguinis in their saliva than do children colonized with mutans streptococci. Collectively, these findings suggest that the colonization of S. sanguinis may influence the subsequent colonization of mutans streptococci, and this in turn may suggest several ecological approaches toward controlling dental caries.
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Smith, David Livingstone, and Ioana Panaitiu. "HORROR SANGUINIS." Common Knowledge 22, no. 1 (January 2016): 69–80. http://dx.doi.org/10.1215/0961754x-3322882.
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Leikola, J., and A. Hässig. "FoundationVox Sanguinis." Vox Sanguinis 67, no. 3 (October 1994): 245. http://dx.doi.org/10.1111/j.1423-0410.1994.tb01245.x.
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Sardia, Sardia, Ma’rufi Ma’rufi, and Muhammad Ilyas. "KEMAMPUAN PENALARAN SISWA SANGUINIS DALAM MEMECAHKAN MASALAH MATEMATIKA DITINJAU BERDASARKAN GENDER." Pedagogy: Jurnal Pendidikan Matematika 5, no. 2 (December 3, 2020): 135–44. http://dx.doi.org/10.30605/pedagogy.v5i2.421.
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Penalaran matematika penting bagi siswa karena berkaitan dengan pemecahan masalah. Penelitian ini bertujuan untuk mendeskripsikan kemampuan penalaran berdasarkan tipe kepribadian Hippocrates dan Gender. Tipe kepribadian menurut Hippocrates terdiri atas sanguinis, koleris, melankolis, dan plegmatis. Arikel ini hanya membahas khusus tipe kepribadian sanguinis. Melalui pendekatan kualitatif dideskripsikan kemampuan penalaran sanguinis laki-laki dan sanguinis perempuan. Hasil penelitian: Sanguinis Laki-laki; mengumpulkan fakta secara lengkap, tidak sempurna dalam mengajukan asumsi, melakukan manipulasi matematika secara jelas dan terurut, memeriksa kesahihan asumsi dengan pengecekan maju, tidak mampu membangun argumen, membuat kesimpulan disertai alasan. Sanguinis Perempuan; mengumpulkan fakta secara lengkap, tidak sempurna dalam mengajukan asumsi, melakukan manipulasi matematika secara jelas dan terurut, memeriksa kesahihan asumsi dengan pengecekan langkah demi langkah, tidak mampu membangun argumen, membuat kesimpulan disertai alasan.
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Hutomo, Suryani, Heni Susilowati, Dewi Agustina, and Widya Asmara. "Analysis of anti-Streptococcus sanguinis IgY ability to inhibit Streptococcus sanguinis adherence." Dental Journal (Majalah Kedokteran Gigi) 51, no. 1 (March 31, 2018): 33. http://dx.doi.org/10.20473/j.djmkg.v51.i1.p33-36.
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Background: Streptococcus sanguinis (S. sanguinis), an oral commensal bacterium, is often implicated in infective endocarditis. Its adherence to the tooth surface is the initial step in dental plaque formation. In addition to the important role of S. sanguinis in systemic disease and antimicrobial resistance, it is necessary to develop methods to control dental plaque formation. Immunoglobulin Y (IgY) has been used to prevent bacterial infection. Purpose: The purpose of this study is to analyze the ability of anti-S. sanguinis IgY antibodies to inhibit S. sanguinis adherence to hydroxyapatite (HA) discs as a model of the tooth surface. Methods: Antibodies were produced by immunizing hens with S. sanguinis suspension. Boosters were given three times following the first injection. An agar gel precipitation test (AGPT) was used to detect the presence of anti-S. sanguinis IgY. A bacterial adherence assay was performed twice to analyze the ability of IgY and the optimal concentration required to inhibit bacterial adherence. Results: The formation of a precipitation line using AGPT confirmed the presence of the antibody. In addition, it was shown that the anti-S. sanguinis IgY antibody could inhibit bacterial adherence to HA. Statistical analysis using One-way ANOVA revealed a significant difference in the optical density (OD) value between the groups (p<0.05). The results of electron microscopy scanning confirmed the quantitative analysis by means of a bacterial adherence test. Conclusion: Anti-S. sanguinis IgY has the ability to inhibit adherence of S. sanguinis to HA discs at an optimal concentration of 30%. The inhibitive effect was stronger in the presence of saliva.
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Nobbs, Angela H., Yongshu Zhang, Ali Khammanivong, and Mark C. Herzberg. "Streptococcus gordonii Hsa Environmentally Constrains Competitive Binding by Streptococcus sanguinis to Saliva-Coated Hydroxyapatite." Journal of Bacteriology 189, no. 8 (February 2, 2007): 3106–14. http://dx.doi.org/10.1128/jb.01535-06.
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ABSTRACT Competition between pioneer colonizing bacteria may determine polymicrobial succession during dental plaque development, but the ecological constraints are poorly understood. For example, more Streptococcus sanguinis than Streptococcus gordonii organisms are consistently isolated from the same intraoral sites, yet S. gordonii fails to be excluded and survives as a species over time. To explain this observation, we hypothesized that S. gordonii could compete with S. sanguinis to adhere to saliva-coated hydroxyapatite (sHA), an in vitro model of the tooth surface. Both species bound similarly to sHA, yet 10- to 50-fold excess S. gordonii DL1 reduced binding of S. sanguinis SK36 by 85 to >95%. S. sanguinis, by contrast, did not significantly compete with S. gordonii to adhere. S. gordonii competed with S. sanguinis more effectively than other species of oral streptococci and depended upon the salivary film on HA. Next, putative S. gordonii adhesins were analyzed for contributions to interspecies competitive binding. Like wild-type S. gordonii, isogenic mutants with mutations in antigen I/II polypeptides (sspAB), amylase-binding proteins (abpAB), and Csh adhesins (cshAB) competed effectively against S. sanguinis. By contrast, an hsa-deficient mutant of S. gordonii showed significantly reduced binding and competitive capabilities, while these properties were restored in an hsa-complemented strain. Thus, Hsa confers a selective advantage to S. gordonii over S. sanguinis in competitive binding to sHA. Hsa expression may, therefore, serve as an environmental constraint against S. sanguinis, enabling S. gordonii to persist within the oral cavity, despite the greater natural prevalence of S. sanguinis in plaque and saliva.
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Mitrakul, Kemthong, Kutkao Vongsawan, Assavinee Sriutai, and Wipaphan Thosathan. "Association between S. mutans and S. sanguinis in Severe Early childhood Caries and Caries-Free Children A Quantitative Real-Time PCR Analysis." Journal of Clinical Pediatric Dentistry 40, no. 4 (October 1, 2016): 281–89. http://dx.doi.org/10.17796/1053-4628-40.4.281.
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Objectives: To identify S. mutans and S. sanguinis in initial and overnight plaque between 2 groups and to analyze the association between them and caries-related factors. Study design: Collected supra gingival plaque from 140 Thai children aged 2–6 years old (S-ECC = 68, caries-free=72). Recorded plaque and gingival indices, dmft score, salivary mutans streptococci level, pH and buffer capacity. Firstly, the overnight plaque was collected, then, 4 hrs. after a thorough prophylaxis, the initial plaque was collected. Accessed parent's attitude and behavior in children's oral hygiene care and diet practice using a questionnaire. A quantitative real-time PCR was performed. Results: For initial plaque, S. sanguinis was higher in caries-free. S. mutans (0.011) and S. mutans/S. sanguinis ratio (0.005) were higher in S-ECC. S. sanguinis amount was inverse correlated with dmft (0.00), gingival index (0.044), and plaque index (0.011). For overnight plaque, S. mutans (0.00) and S. mutans/S. sanguinis ratio (0.005) were also higher in S-ECC. S. mutans, S. mutans/S. sanguinis ratio were positive correlated to dmft (0.00). Parent education levels (0.004) and bottle feeding (0.011) between 2 groups were different. Conclusion: S. sanguinis, S. mutans and their ratio in initial and overnight plaque, low family income and bottle feeding are strongly associated with S-ECC.
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Rogulski, Jakub. "‘Gutullae sanguinis Iagellonici’." Canadian-American Slavic Studies 52, no. 4 (December 7, 2018): 345–68. http://dx.doi.org/10.1163/22102396-05204007.
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AbstractIn the Grand Duchy of Lithuania and (after 1569) the Polish-Lithuanian Commonwealth there lived many princely families who originated (or were supposed to have originated) from the House of Gediminas, the Gediminids. Their descent from this house linked them with the Jagiellonians, who were also Gediminids and who had become the powerful royal dynasty in Poland and Lithuania until 1572 (and other European countries). Using written and visual evidence from the sixteenth to eighteenth centuries, this article argues that real and claimed Jagiellonian descent played a crucial role in the social and political lives of many prominent Lithuanian princely families. Consequently, the princes sought to commemorate their relationship with the dynasty by exploiting forms both of “memory storage” (that is to say documents, genealogies or panegyrics that presented irrefutable information about their Jagiellonian kinship links), and “functional memory” (which included family symbols strongly identified with the Jagiellonians). In this way the princes created their identity as members of the “royal kin of Lithuanian princes” and presented themselves as the junior collateral of the powerful dynasty. This image of the origins of these princely families was in turn transmitted to the future generations, reinforcing their collective identity and their sense of status, and promoting variously their political ambitions.
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Leikola, J., and A. Hässig. "Foundation Vox Sanguinis." Vox Sanguinis 67, no. 3 (1994): 245. http://dx.doi.org/10.1159/000462607.
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Connolly-Weinert, Frank. "Ecce Sanguinis Mei." Theology Today 62, no. 2 (July 2005): 245. http://dx.doi.org/10.1177/004057360506200211.
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Lestari, Oktaviani Suci, Rini Devijanti Ridwan, Tuti Kusumaningsih, and S. Sidarningsih. "The activity of polyclonal IgY derived from Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in inhibiting colonization of Fusobacterium nucleatum and Streptococcus sanguinis." Dental Journal (Majalah Kedokteran Gigi) 52, no. 2 (June 30, 2019): 81. http://dx.doi.org/10.20473/j.djmkg.v52.i2.p81-85.
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Background: Fusobacterium nucleatum (F. nucleatum) and Streptococcus sanguinis (S. sanguinis) play a role in dental plaque formation which leads to periodontitis. Immunoglobulin Y (IgY) is present in both serum and egg yolk and can bind to the surface components of bacteria. F. nucleatum and S. sanguinis feature the same type of IV pili as Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). Saliva binding protein (SsaB) in S. sanguinis is a FimA homolog. FimA constitutes a surface element of Porphyromonas gingivalis (P. gingivalis). F. nucleatum and P. gingivalis possess the same outer membrane protein (OMP) molecular mass. Purpose: The study aimed to determine the activity of A. actinomycetemcomitans and P. gingivalis polyclonal IgY present in serum and egg yolk that can inhibit colonization of F. nucleatum and S. sanguinis. Methods: IgY samples were diluted with phosphate buffer saline (PBS). Several holes were made in the nutrient medium with 10 μl antigen (F. nucleatum/S. sanguinis) being inserted into the center hole. 10 μl PBS, 1:1, 1:2, 1:4, 1:8, 1:16 A. actinomycetemcomitans or P. gingivalis polyclonal IgY were subsequently introduced into the surrounding holes. The results of incubation at 37°C were observed after 24-48 hours. Kruskal Wallis and MannWhitney tests were administered to analyse the data. Results: A. actinomycetemcomitans and P. gingivalis polyclonal IgY groups in serum showed a precipitation line at dilution ratios of 1:1 and 1:2, whereas in egg yolk this occurred only at a 1:1 dilution ratio with F. nucleatum and S. sanguinis bacteria in this study. No significant differences were evident between each dilution (p>0.05) and none existed between serum and egg yolk (p>0.05). Conclusion: IgY polyclonal of A. actinomycetemcomitans and P. gingivalis in both serum and egg yolk initiate activities that can inhibit colonization of F. nucleatum and S. sanguinis.
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Busri, Ajrina, Ria Puspitawati, and Sri Utami. "ANTIBACTERIAL EFFECT OF JAVA TURMERIC ETHANOL EXTRACT AGAINST DUAL-SPECIES STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SANGUINIS BIOFILM (IN VITRO)." Asian Journal of Pharmaceutical and Clinical Research 10, no. 17 (October 1, 2017): 57. http://dx.doi.org/10.22159/ajpcr.2017.v10s5.23096.
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Objectives: The minimal bactericidal concentration (MBC) of Java turmeric (Curcuma xanthorrhiza Roxb.) ethanol extract is 25% against Streptococcus mutans and 15% against Streptococcus sanguinis as single species. This study aimed to examine the antibacterial effect of Java turmeric ethanol extract against S. mutans and S. sanguinis as dual-species. S. mutans and S. sanguinis compete against each other to obtain nutrients.Methods: The antibacterial effect of Java turmeric ethanol extract against dual-species Streptococcus in vitro was analyzed by measuring the growth of bacteria after exposure to the extract by counting colony formation and quantifying bacterial cell numbers using real-time polymerase chain reaction.Result: The MBC of Java turmeric ethanol extract against dual-species Streptococcus is 10%. S. sanguinis is more sensitive to the extract than S. mutans. Conclusions: The antibacterial effect of Java turmeric ethanol extract on S. mutans and S. sanguinis as single species differs from the effect on the bacteria as dual-species of Streptococcus.
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Lozano, Carla P., Natalia Díaz-Garrido, Jens Kreth, and Rodrigo A. Giacaman. "Streptococcus mutans and Streptococcus sanguinis Expression of Competition-Related Genes, Under Sucrose." Caries Research 53, no. 2 (August 14, 2018): 194–203. http://dx.doi.org/10.1159/000490950.
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Streptococcus mutans synthesizes 3 glucosyltransferases (Gtfs) associated with cariogenic biofilms, while commensal Streptococcus sanguinis produces only one; gtfP and hydrogen peroxide (H2O2) by SpxB. The aim was to test the hypothesis that under a sucrose-induced cariogenic challenge, the expression of competition-related genes is differentially regulated depending on whether S. sanguinis or S. mutans primarily colonize enamel. Dual-species biofilms of S. sanguinis and S. mutans were formed under different colonization sequences on enamel slabs and exposed to 10% sucrose for 5 min, 3×/day for 5 days. Biofilms were analyzed for the transcriptional response of competition-related genes encoding gtfB, gtfC, and gtfD for S. mutans and gtfP and spxB for S. sanguinis. In addition, acidogenicity (pH) and viable cells in each of the conditions were determined. For all the genes, a downregulation was observed during simultaneous colonization by both bacterial species. In contrast, gtfB was upregulated when S. sanguinis was the first colonizer (p < 0.05). Both gtfC and gtfD were upregulated during sequential inoculation with S. sanguinis as the first colonizer. An eleven-fold upregulation of gtfP was observed in biofilms with S. mutans as initial colonizer (p < 0.05), with a moderate increase in spxB expression. The lowest pH values and viable cells of S. sanguinis were observed when S. mutans first colonized the enamel slabs, compared to the other conditions (p < 0.05). Demanding sucrose-challenged oral environment requires increased expression of virulence traits to effectively compete and thrive in the dental biofilm, especially when the competitor has already colonized the ecological niche.
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Jain, Neetu, Purva Mathur, and Mahesh Chandra Misra. "Globicatella sanguinis meningitis in a post head trauma patient: first case report from Asia." Journal of Infection in Developing Countries 6, no. 07 (July 20, 2012): 592–94. http://dx.doi.org/10.3855/jidc.1947.
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Globicatella sanguinis is a rare isolate in clinical samples. We present a case of meningitis in a 70-year-old male patient after a head injury operation. Three consecutive cerebrospinal fluid samples obtained from the patient identified Globicatella sanguinis based on morphology, biochemical profile, and Vitek-2 identification. The patient recovered after antibiotic treatment with vancomycin. This is the first case report of Globicatella sanguinis from Asia from a case of meningitis.
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Ge, Xiuchun, Todd Kitten, Zhenming Chen, Sehmi P. Lee, Cindy L. Munro, and Ping Xu. "Identification of Streptococcus sanguinis Genes Required for Biofilm Formation and Examination of Their Role in Endocarditis Virulence." Infection and Immunity 76, no. 6 (April 7, 2008): 2551–59. http://dx.doi.org/10.1128/iai.00338-08.
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ABSTRACT Streptococcus sanguinis is one of the pioneers in the bacterial colonization of teeth and is one of the most abundant species in the oral biofilm called dental plaque. S. sanguinis is also the most common viridans group streptococcal species implicated in infective endocarditis. To investigate the association of biofilm and endocarditis, we established a biofilm assay and examined biofilm formation with a signature-tagged mutagenesis library of S. sanguinis. Four genes that have not previously been associated with biofilm formation in any other bacterium, purB, purL, thrB, and pyrE, were putatively identified as contributing to in vitro biofilm formation in S. sanguinis. By examining 800 mutants for attenuation in the rabbit endocarditis model and for reduction in biofilm formation in vitro, we found some mutants that were both biofilm defective and attenuated for endocarditis. However, we also identified mutants with only reduced biofilm formation or with only attenuation in the endocarditis model. This result indicates that the ability to form biofilms in vitro is not associated with endocarditis virulence in vivo in S. sanguinis.
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Ashby, Michael T., Jens Kreth, Muthu Soundarajan, and Laure Sita Sivuilu. "Influence of a model human defensive peroxidase system on oral streptococcal antagonism." Microbiology 155, no. 11 (November 1, 2009): 3691–700. http://dx.doi.org/10.1099/mic.0.031310-0.
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Streptococcus is a dominant genus in the human oral cavity, making up about 20 % of the more than 800 species of bacteria that have been identified, and about 80 % of the early biofilm colonizers. Oral streptococci include both health-compatible (e.g. Streptococcus gordonii and Streptococcus sanguinis) and pathogenic strains (e.g. the cariogenic Streptococcus mutans). Because the streptococci have similar metabolic requirements, they have developed defence strategies that lead to antagonism (also known as bacterial interference). S. mutans expresses bacteriocins that are cytotoxic toward S. gordonii and S. sanguinis, whereas S. gordonii and S. sanguinis differentially produce H2O2 (under aerobic growth conditions), which is relatively toxic toward S. mutans. Superimposed on the inter-bacterial combat are the effects of the host defensive mechanisms. We report here on the multifarious effects of bovine lactoperoxidase (bLPO) on the antagonism between S. gordonii and S. sanguinis versus S. mutans. Some of the effects are apparently counterproductive with respect to maintaining a health-compatible population of streptococci. For example, the bLPO system (comprised of bLPO+SCN−+H2O2) destroys H2O2, thereby abolishing the ability of S. gordonii and S. sanguinis to inhibit the growth of S. mutans. Furthermore, bLPO protein (with or without its substrate) inhibits bacterial growth in a biofilm assay, but sucrose negates the inhibitory effects of the bLPO protein, thereby facilitating adherence of S. mutans in lieu of S. gordonii and S. sanguinis. Our findings may be relevant to environmental pressures that select early supragingival colonizers.
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Shallal, Lubna F., and Maha A. Ahmed. "Experimental In vitro Study to Assess the Antibacte-rial Activity of Thymus vulgaris Oil on Streptococ-cus Sanguinis." Journal of Baghdad College of Dentistry 34, no. 4 (December 15, 2022): 17–27. http://dx.doi.org/10.26477/jbcd.v34i4.3273.
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Background: The Streptococcus genus are the predominant bacteria in the mouth and the Streptococcus sanguinis is one of them which performing a primary function for expansion of dental biofilm. Gingival and periodontal disorders are caused by dental biofilm, today, there is a necessity to discover naturally presenting antibacterial compounds from herbs with less side effects as a substitutive to the commonly handled chlorohexidine. Thus, the purpose of this study was to assess the antibacterial activity of thymus vulgaris oil on Streptococcus sanguinis bacteria In vitro. Materials and Methods: Human supragingival plaque samples were taken from 10 subjects, then morphological and microscopical examination, biochemical tests, Optochin test, hemolytic ability test and conventional polymerase chain reaction test was applied to assure coincidence of Streptococcus sanguinis. The sensitivity of bacteria to Thymus vulgaris oil, the minimum concentration that inhibit the growth and killed the test bacteria were identified a partly as comparable to 0.2% chlorhexidine as a positive control and10 % dimethyl sulphoxide as a negative control. Results: Thymus vulgaris oil presented a significant antibacterial property on Streptococcus sanguinis with several grades of inhibition zones. When compared to Chlorohexidine, Thymus vulgaris oil had stronger antibacterial properties. The minimum concentration that inhibited the growth and killed Streptococcus sanguinis was (0.09%). Conclusion: Thymus vulgaris oil displayed higher antibacterial action with each concentration on Streptococcus sanguinis as compared to Chlorohexidine, therefore; it can be used as a natural substitutive oral health care product to Chlorohexidine.
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Verdier, Marie-Hélène. "Sanguines." Pierre d'angle 8 (2002): 91–101. http://dx.doi.org/10.5840/pda200288.
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Díaz Martínez, Sergio, Jorge Luciano Villán Gaona, Samuel Vega Hurtado, Diana Patricia Pachón Barinas, and Alberto Fabio Caballero Restrepo. "Globicatella sanguinis: reporte de un caso de meningitis en el Hospital Militar Central." Revista Med 25, no. 1 (June 25, 2017): 58–63. http://dx.doi.org/10.18359/rmed.2918.
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Globicatella sanguinis es un microorganismo patógeno poco común, coco Gram positivo y catalasa negativo, aunque se aísla en ganado ovino, ha sido descrito como agente infeccioso causante de meningitis en seres humanos. La primera descripción de este patógeno fue realizada por Collins y colaboradores en el año de 1992, se debe resaltar, que antes de este suceso, parte de los casos reportados como meningitis por S. viridans, pudieron ser causados en realidad por G. sanguinis debido a la falta de identificación de este ultimo y a la gran similitud en cuanto a morfología y resultados de pruebas bioquímicas. Al respecto, desde la descripción original hace 20 años, la información disponible de G. sanguinis es escasa. Se ha presentado un caso de meningitis asociada a G. sanguinis posterior a un procedimiento quirúrgico en un paciente de 1 año de edad en el Hospital Militar Central.
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Xu, Ping, Joao M. Alves, Todd Kitten, Arunsri Brown, Zhenming Chen, Luiz S. Ozaki, Patricio Manque, et al. "Genome of the Opportunistic Pathogen Streptococcus sanguinis." Journal of Bacteriology 189, no. 8 (February 2, 2007): 3166–75. http://dx.doi.org/10.1128/jb.01808-06.
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ABSTRACT The genome of Streptococcus sanguinis is a circular DNA molecule consisting of 2,388,435 bp and is 177 to 590 kb larger than the other 21 streptococcal genomes that have been sequenced. The G+C content of the S. sanguinis genome is 43.4%, which is considerably higher than the G+C contents of other streptococci. The genome encodes 2,274 predicted proteins, 61 tRNAs, and four rRNA operons. A 70-kb region encoding pathways for vitamin B12 biosynthesis and degradation of ethanolamine and propanediol was apparently acquired by horizontal gene transfer. The gene complement suggests new hypotheses for the pathogenesis and virulence of S. sanguinis and differs from the gene complements of other pathogenic and nonpathogenic streptococci. In particular, S. sanguinis possesses a remarkable abundance of putative surface proteins, which may permit it to be a primary colonizer of the oral cavity and agent of streptococcal endocarditis and infection in neutropenic patients.
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Nasution, Aini Hariyani, Yulianti Kemal, Robert Lessang, and Boy Bachtiar. "QUANTIFICATION OF STREPTOCOCCUS SANGUINIS ISOLATED FROM DENTAL PLAQUE AND SALIVA OF SUBJECTS WITH AND WITHOUT CORONARY HEART DISEASE – ANALYSIS USING REAL-TIME PCR." Dentika Dental Journal 20, no. 1 (July 17, 2017): 13–19. http://dx.doi.org/10.32734/dentika.v20i1.645.
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Coronary Heart Disease (CHD) is the major cause of death in most countries in the world. Gram-positive and Gram-negative bacteria have been identified in bacteremia cases and known to have a role in various vascular diseases, including Streptococcus sanguinis which is most frequently isolated from endocarditis patients and often associated with CHD. The purpose of this study was to analyze the number of Streptococcus sanguinis isolated from dental plaque and saliva of subjects with and without CHD. Bacterial colonies isolated from the dental plaque and saliva of 16 subjects without CHD and 8 subjects with CHD were planted in Mitis salivarius agar, and then the DNA was extracted and quantified with a Real-Time PCR technique using 16S rRNA specific primers. The quantification of Real-Time PCR showed that there was a difference in the number of S. sanguinis between the two groups of subjects, but an unpaired T-test showed that the difference was not statistically significant. Furthermore, the number of S. sanguinis from dental plaque in CHD subjects tends to be higher than that of non-CHD subjects whereas the number of S. sanguinis from saliva in non-CHD subjects tends to be higher than that of CHD subjects.
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Wulandari, Nila Murni, Edhie Arif Prasetyo, Agus Subiwahjudi, and Tamara Yuanita. "The Difference Of Antibacterial Power Between Cocoa Peel (Theobroma cacao L.) Extract 6,25% and Chlorhexidine 0,2% Againts Streptococcus sanguinis." Conservative Dentistry Journal 9, no. 1 (December 5, 2019): 40. http://dx.doi.org/10.20473/cdj.v9i1.2019.40-47.
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Background: Streptococcus sanguinis is a bacterium that can cause failures in root canal treatments due its ability to penetrate the dentinal tubules to a depth of 400 μm in just two weeks. Irrigation material is needed to stop the growth of this bacteria so that no bacteria can pass through by using chemicals, irrigation materials that are widely used such as Chlorhexidine 0,2% but still lack because it cannot be used as a single irrigation solution because its effectiveness will be reduced if it is related to protein and organic dentine matrix and low Ph saliva.Therefore, research is needed to find natural ingredient that can be an alternative such as Cocoa peel extract was chosen because it contains active compounds, in the form of saponins, tanins, alkaloids, flavonoids, and terpenoids that have been known to have antibacterial properties a concentration of 6,25% is used in accordance with the MKC of Streptococcus sanguinis. Aim: To compare the antibacterial power between cocoa peel extract (Theobroma cacao L.) 6,25% and Chlorhexidine 0,2% against Streptococcus sanguinis Method: This research is an experimental laboratory with a post-test only control group design. The diffusion method was used to determine the susceptibility of bacteria isolated from the material by planting the culture of Streptococcus sanguinis on the agar medium by swabbing the nutrient media which has been divided into 3 parts consisting of negative control, cocoa peel extract and Chlorhexidine, then each nurient media so that it is given a paper disk and 0.01ml liquid on each section. The diameter of the inhibition zone was observed after 2x24 hours using the calipers. Results: The average inhibitory zone that was formed using cocoa peel extract was 20,40 mm against Streptococcus sanguinis and Chlorhexidine was 18,36 against Streptococcus sanguinis. Conclusion: Cocoa peel extract (Theobroma cacao L.) 6,25% had higher anti-bacterial power compared to 0,2% Chlorhexidine against the growth of Streptococcus sanguinis.
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Lockwood, Jeffrey A. "TAXONOMIC STATUS OF THE ROCKY MOUNTAIN LOCUST: MORPHOMETRIC COMPARISONS OF MELANOPLUS SPRETUS (WALSH) WITH SOLITARY AND MIGRATORY MELANOPLUS SANGUINIPES (F.)." Canadian Entomologist 121, no. 12 (December 1989): 1103–9. http://dx.doi.org/10.4039/ent1211103-12.
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AbstractMorphometric analysis of 16 body measurements of migratory and solitary Melanoplus sanguinipes (F.) and M. spretus (Walsh) was used to determine if M. spretus was, in fact, the migratory phase of M. sanguinipes. Migratory M. sanguinipes was found to be more similar to its own solitary phase than to M. spretus. Melanoplus spretus resembled migratory M. sanguinipes, but in 14 comparisons M. spretus differed significantly from both solitary and migratory M. sanguinipes. Despite the potential for environmental variation obscuring differences in morphology, the divergent morphometric characters of M. spretus and the phases of M. sanguinipes appear to justify the status of M. spretus as a true species.
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Nugroho, Sepdhyo Wahyu, Mandojo Rukmo, Edhie Arief Prasetyo, and Tamara Yuanita. "Antibakteri Ekstrak Kulit Buah Kakao (Theobroma cacao) 6,25% dan NaOCl 2,5% Terhadap Bakteri Streptococcus sanguinis." Conservative Dentistry Journal 9, no. 1 (December 5, 2019): 19. http://dx.doi.org/10.20473/cdj.v9i1.2019.19-21.
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Background: Streptococcus sanguinis is a gram-positive bacterium that infects and penetrates into dentinal tubules from a depth of 150 μm up to 792 μm. Chemicals, namely 2.5% NaOCl, are used to mitigate the growth of these bacteria via irrigation of the root canals, but 2.5% NaOCl still has disadvantages including irritating periradicular tissue, having an unpleasant odor, and being toxic. Due to these shortcomings, natural materials are expected to be used as alternatives. Cocoa peel extract has active tannin compounds, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial attributes; a concentration of 6.25% is counted as the minimum bactericidal concentration (MBC) of Streptococcus sanguinis. Aim: To compare the difference of antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) and 2.5% NaOCl against Streptococcus sanguinis. Method: This research is an in vitro experimental laboratory with post-test only control group design. The diffusion method was used on Streptococcus sanguinis planted in tubes containing BHIB, then cultured on petri dishes containing nutrient agar and divided into 3 parts, namely 6.25% cocoa peel extract, 2.5% NaOCl, and negative controls, then each Petri dishes were given a paper disc that had been dripped by 0.01 ml of each ingredient, then incubated in an incubator for 2x24 hours at 37°C; the diameter of the inhibitory zone formed was then observed using a caliper. Results: The average diameter of the inhibition zone formed on 6.25% cocoa peel extract was 19.2000 mm and 2.5% NaOCl was 17.2813 mm against Streptococcus sanguinis. Conclusion: The antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) is higher than 2.5% NaOCl against Streptococcus sanguinis.
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Brañez Reyes, Katherine, Donald Ramos-Perfecto, Américo Castro Luna, César Piscoche Botello, Darío Dávila Paredes, and Juan Celidonio Ruiz Macedo. "Efecto antibacteriano in vitro del extracto de Stevia rebaudiana sobre Streptococcus sanguinis y Actinomyces viscosus, bacterias iniciadoras en la formación de biopelícula dental." Odontología Sanmarquina 21, no. 1 (March 21, 2018): 21. http://dx.doi.org/10.15381/os.v21i1.14428.
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Objetivo: Determinar el efecto antibacteriano del extracto de Stevia rebaudiana (S. rebaudiana) frente a Streptococcus sanguinis (S. sanguinis) y Actinomyces viscosus (A. viscosus). Métodos: Se desarrolló la prueba de sensibilidad en placa de agar con discos, para lo cual se cultivaron las cepas de S. sanguinis y A. viscosus en placas de agar tripticasa soya (TSA) y agar sangre respectivamente, incubando a 37 °C por 48 horas a S. sanguinis y por 7 días en condiciones de anaerobiosis a A. viscosus. Las cepas bacterianas fueron estandarizadas a una escala de 0,5 de Mc Farland, y tomando inóculos de 100 μL fueron sembradas en diez placas de agar sangre y TSA, luego sobre cada placa se colocaron los discos de papel secante de 6 mm de diámetro de forma equidistante, cargados con 10 μL de las diferentes concentraciones del extracto, para luego ser incubados. Resultados: Las concentraciones de 15, 30, 50, 60 y 120 mg/ml presentaron un halo de inhibición promedio de 6,8±0,258; 8,2±1,15; 8,2±1,13; 8,3±0,823; 8,1±0,80 mm respectivamente, para las bacterias de S. sanguinis. Las concentraciones de 15, 30, 50, 60 y 120 mg/ml presentaron un halo de inhibición promedio de 7,2±0,75; 9,65±2,15; 9,20±2,03; 7,95±1,09; 8,05±0,96 mm respectivamente, para las bacterias de A. viscosus. La prueba de Kruskal Wallis determinó que existe diferencia estadísticamente significativa con p<0,05 de los promedios entre las concentraciones de A. viscosus. Conclusiones: El extracto de S. rebaudiana no presenta efecto antibacteriano para S. sanguinis, pero si presenta efecto antibacteriano sobre A. viscosus para las concentraciones de 30 y 50 mg/ml.
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Mayr, W. R., and P. F. W. Strengers. "Vox Sanguinis - 100 Volumes." Vox Sanguinis 100, no. 1 (December 22, 2010): 1. http://dx.doi.org/10.1111/j.1423-0410.2010.01447.x.
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Moraes, Julianna J., Rafael N. Stipp, Erika N. Harth-Chu, Tarsila M. Camargo, José F. Höfling, and Renata O. Mattos-Graner. "Two-Component System VicRK Regulates Functions Associated with Establishment of Streptococcus sanguinis in Biofilms." Infection and Immunity 82, no. 12 (September 2, 2014): 4941–51. http://dx.doi.org/10.1128/iai.01850-14.
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ABSTRACTStreptococcus sanguinisis a commensal pioneer colonizer of teeth and an opportunistic pathogen of infectious endocarditis. The establishment ofS. sanguinisin host sites likely requires dynamic fitting of the cell wall in response to local stimuli. In this study, we investigated the two-component system (TCS) VicRK inS. sanguinis(VicRKSs), which regulates genes of cell wall biogenesis, biofilm formation, and virulence in opportunistic pathogens. AvicKknockout mutant obtained from strain SK36 (SKvic) showed slight reductions in aerobic growth and resistance to oxidative stress but an impaired ability to form biofilms, a phenotype restored in the complemented mutant. The biofilm-defective phenotype was associated with reduced amounts of extracellular DNA during aerobic growth, with reduced production of H2O2, a metabolic product associated with DNA release, and with inhibitory capacity ofS. sanguiniscompetitor species. No changes in autolysis or cell surface hydrophobicity were detected in SKvic. Reverse transcription-quantitative PCR (RT-qPCR), electrophoretic mobility shift assays (EMSA), and promoter sequence analyses revealed that VicR directly regulates genes encoding murein hydrolases (SSA_0094,cwdP, andgbpB) andspxB, which encodes pyruvate oxidase for H2O2production. Genes previously associated withspxBexpression (spxR,ccpA,ackA, andtpK) were not transcriptionally affected in SKvic. RT-qPCR analyses ofS. sanguinisbiofilm cells further showed upregulation of VicRK targets (spxB,gbpB, andSSA_0094) and other genes for biofilm formation (gtfPandcomE) compared to expression in planktonic cells. This study provides evidence that VicRKSsregulates functions crucial forS. sanguinisestablishment in biofilms and identifies novel VicRK targets potentially involved in hydrolytic activities of the cell wall required for these functions.
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Millo, Grazielle, Apa Juntavee, Ariya Ratanathongkam, Natsajee Nualkaew, Peerapattana, Jomjai, and Supaporn Chatchiwiwattana. "Antibacterial Inhibitory Effects of Punica Granatum Gel on Cariogenic Bacteria: An in vitro Study." International Journal of Clinical Pediatric Dentistry 10, no. 2 (2017): 152–57. http://dx.doi.org/10.5005/jp-journals-10005-1426.
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ABSTRACT Aim This study evaluated the in vitro antibacterial effects of the formulated Punica granatum (PG) gel against Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus casei. Materials and methods The PG extract was dissolved in water at 500 mg/mL. High performance liquid chromatography (HPLC) was used for identification and quantification of chemical marker punicalagin. Minimum bactericidal concentration (MBC) and time-kill assay (TKA) were investigated. Antibacterial activities of the formulated PG gel, 2% chlorhexidine (CHX) gel and blank gel were tested by measuring the zones of inhibition through agar well diffusion method. Results The HPLC results showed presence of punicalagin at 2023.58 ± 25.29 μg/mL in the aqueous PG extract and at 0.234% (w/w) in the formulated PG gel. The MBC for S. mutans, S. Sanguinis, and L. casei were 250, 125, and 500 mg/mL respectively. The TKA of 500 mg/mL aqueous PG extract showed total inhibition of S. mutans, S. Sanguinis, and L. casei at 6, 1, and 24 hours contact time respectively. Agar well diffusion revealed that for S. mutans, CHX gel > PG gel > blank gel; for S. sanguinis, CHX gel = PG gel > blank gel; for L. casei, CHX gel > PG gel = blank gel. Comparison of the PG gel potency showed that S. sanguinis = S. mutans > L. casei. Conclusion The PG gel equivalent to 0.234% punicalagin (w/w) inhibited S. mutans and S. sanguinis but not L. casei within 24 hours incubation period and has the potential to be used for caries prevention. How to cite this article Millo G, Juntavee A, Ratanathongkam A, Nualkaew N, Peerapattana J, Chatchiwiwattana S. Antibacterial Inhibitory Effects of Punica Granatum Gel on Cariogenic Bacteria: An in vitro Study. Int J Clin Pediatr Dent 2017;10(2):152-157.
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Sri Praptiningsih, Rahmawati, Sheilia Siwi Pranantri, and Anggun Feranisa. "EFFECT OF THE PROBIOTIC STREPTOCOCCUS SANGUINIS ON THE FORMATION OF STREPTOCOCCUS MUTANS BIOFILM IN ARTIFICIAL SALIVA." Dentino : Jurnal Kedokteran Gigi 7, no. 2 (October 28, 2022): 124. http://dx.doi.org/10.20527/dentino.v7i2.14616.
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Background: Dental caries is a disease of the hard tissues of the teeth that most often occurs with a prevalence rate of about 45.3%. Dental caries is occuring because of the interaction between biofilms and carbohydrates from the food on the tooth surface (host). The caused by the biofilm formation is the presence of colonies of Streptococcus mutans bacteria. To reduce the accumulation of biofilms, mechanical and chemical methods can be used, namely using a toothbrush and mouthwash. Probiotics are widely used in dentistry because they have anti-plaque activity and form H2O2 which can reduce biofilm formation. Purpose: The purpose of this study was to determine the effect of the probiotic Streptococcus sanguinis on the formation of Streptococcus mutans biofilm in artificial saliva. Method: This research method was experimental analytic with post test only group design. The samples were 9 and divided into 3 groups, namely the probiotic group, Streptococcus sanguinis, the positive control group chlorhexidine and the negative placebo control group. Each group incubated in adhesion phase for 4 hours at 37°C. The formation of biofilms is measured by calculating the average results of Optical Density using an ELISA-reader. Data analysis was performed using the test One-way Anova. Results: The average values of optical density in the S. sanguinis, chlorhexidine and placebo groups, in sequence, were 0.374, 0.414, and 0.420. One-way Anova test obtained a significant value (p) of 0.014 (p<0.05) which indicates that there are significant differences in the probiotic group Streptococcus sanguinis, the chlorhexidine group, and the placebo group. Conclusion: This study showed that the probiotic Streptococcus sanguinis had a significant impact on the formation of Streptococcus mutans biofilm in artificial saliva. Keywords : Biofilm, Optical Density, Probiotic, Streptococcus mutans, Streptococcus sanguinis
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Rozanna, Rozanna, Nurtami Soedarsono, and Ratna Farida. "EFFECT OF PROPOLIS EXTRACT AND PROPOLIS CANDIES ON THE GROWTH OF STREPTOCOCCUS SANGUINIS ATCC 10556." Asian Journal of Pharmaceutical and Clinical Research 10, no. 17 (October 1, 2017): 16. http://dx.doi.org/10.22159/ajpcr.2017.v10s5.23083.
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Objectives: This study aimed to analyze the effects of propolis extract and propolis candies on Streptococcus sanguinis.Methods: Bacterial suspensions were exposed to propolis extract and propolis candies. For propolis extract, a minimum inhibitory concentration(MIC) was determined using a spectrophotometer, and a minimum bactericidal concentration (MBC) was determined using a standard plate count method. Samples were cultured in brain heart infusion agar, incubated for 24 hrs, and the bacterial colony units formed on the agar plates were manually counted.Results: Propolis extract inhibits the growth of S. sanguinis at MIC 5% and MBC 10%. The colonies of S. sanguinis decreased after exposure to propolis candies.Conclusions: Propolis extract and propolis candies are effective to inhibit the growth of S. sanguinis.
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Glazunova, Olga O., Didier Raoult, and Véronique Roux. "Streptococcus massiliensis sp. nov., isolated from a patient blood culture." International Journal of Systematic and Evolutionary Microbiology 56, no. 5 (May 1, 2006): 1127–31. http://dx.doi.org/10.1099/ijs.0.64009-0.
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An unidentified strain of the viridans group of streptococci was isolated from a human blood sample. It was distinguished from all other recognized species of the Streptococcus sanguinis group by several biochemical characteristics. Phylogenetic analysis based on 16S rRNA gene sequence comparisons clustered this strain with Streptococcus ferus (mutans group) but phylogenetic analysis based on rpoB and sodA gene sequence comparisons included it in the S. sanguinis group. The isolate showed 95.4 and 95.2 % 16S rRNA gene sequence similarity to S. ferus and S. sanguinis, respectively, confirming it as belonging to a novel taxon, for which the name Streptococcus massiliensis sp. nov. is proposed. The type strain is 4401825T (=CIP 108498T=CCUG 49690T).
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Vela, Ana I., Elena Fernández, Alfonso las Heras, Paul A. Lawson, Lucas Domínguez, Matthew D. Collins, and Jose F. Fernandez-Garayzabal. "Meningoencephalitis Associated withGlobicatella sanguinis Infection in Lambs." Journal of Clinical Microbiology 38, no. 11 (2000): 4254–55. http://dx.doi.org/10.1128/jcm.38.11.4254-4255.2000.
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Thirty lambs displayed symptoms of meningoencephalitis. An unusual gram-positive coccus was isolated in pure culture from the blood and brain samples from one of the affected animals, and phenotypic and phylogenetic characterization showed this to be Globicatella sanguinis. This is the first report of the isolation of G. sanguinis in pure culture from an animal infection.
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Nogueira, Larissa Rosa, Daniela Gabriel Regianni, Adriano Pinter, and Jonas Moraes Filho. "Rhipicephalus sanguineus complex: Review." Brazilian Journal of Global Health 1, no. 2 (February 27, 2021): 28–31. http://dx.doi.org/10.56242/globalhealth;1;2;28-31.
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OBJECTIVE: The present study aimed to carry out a systematic review in order to analyze available information of the Rhipicephalus sanguineus Complex. METHODS: A bibliographic review was carried out, through a research on public scientific articles databases, between August 2019 and January 2020. Search was filtered for scientific articles published between 2000 and 2020, in the search engines: SCIELO, PubMed and Google Scholar, following the guiding question: Rhipicephalus sanguineus complex? and using the descriptors: Rhipicephalus; Rhipicephalus sanguineus; Rhipicephalus sanguineus complex; Phylogeny Rhipicephalus; Rhipicephalus turanicus, Hemoparasitosis. RESULTS: The genus Rhipicephalus comprises a group called Rhipicephalus sanguineus complex, consisting of 17 species that are morphologically similar and that do not yet have an elucidated taxonomy determination, according to several authors, the tick species in question have their biosystematic state of difficult elucidation, as there are biological and genetic divergences. The present publication review yielded support to the hypotheses that the R. sanguineus taxon in America is, in fact, composed by two different lineages, that is nowadays attributed to the R. sanguineus species, but, as a matter of fact, the taxonomic status of R. sanguineus and R. turanicus in the Old World must be defined prior a possible new classification of the New World lineages of R. saguineus, therefore the Ripichephalus genus ticks in the Americas should all be identified as R. saguineus complex. CONCLUSION: Conclude that the R. sanguineus complex encompasses taxonomic, phylogenetic, morphological and biological differences, making its biosystematic state difficult to clarify.
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Fielding, Dennis J., M. A. Brusven, Bahman Shafii, and William J. Price. "Spatial heterogeneity of low-density populations of Melanoplus sanguinipes (Orthoptera: Acrididae) associated with grazing and vegetation treatments." Canadian Entomologist 133, no. 6 (December 2001): 843–55. http://dx.doi.org/10.4039/ent133843-6.
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AbstractThe objectives of this study were to determine whether the spatial distribution of Melanoplus sanguinipes F., the most abundant species of grasshopper on rangeland in southern Idaho, varied annually in response to changing patterns of grazing and to investigate how vegetation affects the spatial distribution of low-density populations of M. sanguinipes at scales relevant to most rangeland-management activities. A lattice of 72 sites was established across nine pastures, covering approximately 5000 ha. At each site, densities of M. sanguinipes, percent canopy coverage by plant species, and percent forage utilization by livestock were estimated twice per year, in June when M. sanguinipes was in the nymphal stage and in August during the adult stage, for 4 years, 1991–1994. Spatial analyses of variance were used to evaluate the influence of grazing and vegetation type on densities of M. sanguinipes. In August of each year, densities of M. sanguinipes were lower on heavily grazed sites than on lightly grazed sites, except in 1993, when the opposite trend was observed. Above-normal precipitation in 1993 resulted in abundant growth of annual forbs and regrowth of grazed plants. The distribution of nymphs in June of 1993 and 1994 reflected the grazing patterns of the previous summer. Densities of M. sanguinipes were lower on crested wheatgrass habitats than on annual grasslands for every sampling period from June 1991 to June 1993, after which no differences were observed. We interpret the results to suggest that grazing effects on low-density populations of M. sanguinipes were contingent on weather conditions; under dry conditions, grazed habitats were less favorable to M. sanguinipes but, during relatively cool wet summers, grazing created conditions that were more favorable to M. sanguinipes.
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Fujishima, Kei, Miki Kawada-Matsuo, Yuichi Oogai, Masayuki Tokuda, Mitsuo Torii, and Hitoshi Komatsuzawa. "dprandsodin Streptococcus mutans Are Involved in Coexistence with S. sanguinis, and PerR Is Associated with Resistance to H2O2." Applied and Environmental Microbiology 79, no. 5 (December 21, 2012): 1436–43. http://dx.doi.org/10.1128/aem.03306-12.
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ABSTRACTLarge numbers of bacteria coexist in the oral cavity.Streptococcus sanguinis, one of the major bacteria in dental plaque, produces hydrogen peroxide (H2O2), which interferes with the growth of other bacteria.Streptococcus mutans, a cariogenic bacterium, can coexist withS. sanguinisin dental plaque, but to do so, it needs a means of detoxifying the H2O2produced byS. sanguinis. In this study, we investigated the association of three oxidative stress factors, Dpr, superoxide dismutase (SOD), and AhpCF, with the resistance ofS. sanguinisto H2O2. The knockout ofdprandsodsignificantly increased susceptibility to H2O2, while the knockout ofahpCFhad no apparent effect on susceptibility. In particular,dprinactivation resulted in hypersensitivity to H2O2. Next, we sought to identify the factor(s) involved in the regulation of these oxidative stress genes and found that PerR negatively regulateddprexpression. The knockout ofperRcaused increaseddprexpression levels, resulting in low-level susceptibility to H2O2compared with the wild type. Furthermore, we evaluated the roles ofperR,dpr, andsodwhenS. mutanswas cocultured withS. sanguinis. Culturing of thedprorsodmutant withS. sanguinisshowed a significant decrease in theS. mutanspopulation ratio compared with the wild type, while theperRmutant increased the ratio. Our results suggest thatdprandsodinS. mutansare involved in coexistence withS. sanguinis, and PerR is associated with resistance to H2O2in regulating the expression of Dpr.
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Stingu, Catalina-Suzana, Klaus Eschrich, Arne C. Rodloff, Reiner Schaumann, and Holger Jentsch. "Periodontitis is associated with a loss of colonization by Streptococcus sanguinis." Journal of Medical Microbiology 57, no. 4 (April 1, 2008): 495–99. http://dx.doi.org/10.1099/jmm.0.47649-0.
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The aim of this study was to estimate differences in the prevalence of oral streptococcal species in the subgingival biofilm of patients with aggressive periodontitis and of healthy controls. Thirty-three patients with clinical and radiological proof of aggressive periodontitis and 20 healthy subjects were enrolled in this study. Clinical indices were recorded in a six-point measurement per tooth. Samples of the subgingival biofilm were taken with paper points from four teeth of each individual. Alpha- and non-haemolytic, small and catalase-negative colonies were biochemically identified using a rapid ID 32 STREP system and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A total of 118 strains of oral streptococci (11 species) were identified and Streptococcus sanguinis was found significantly more often in healthy subjects (P=0.001). Conversely, the absence of S. sanguinis was associated with high values of clinical indices (P=0.001–0.002). Aggressive periodontitis seems to be associated with a loss of colonization of S. sanguinis. Whether or not S. sanguinis offers protection against aggressive periodontitis needs to be determined. Otherwise, there were no significant differences in the distribution of oral streptococcal species in patients and healthy subjects.
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Pradayani, Made Prashanti, Ni Kadek Fiora Rena Pertiwi, and IGA Dyah Ambarawati. "Daya antibakteri ekstrak kulit apel manalagi (Malus sylvestris (L.) Mill) terhadap pertumbuhan Streptococcus sanguinis." Bali Dental Journal 5, no. 2 (July 21, 2021): 63–68. http://dx.doi.org/10.51559/bdj.v5i2.145.
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Introduction: Streptococcus sanguinis is one of the gram-positive bacteria that causes dental and oral diseases that play a role in the process of dental plaque formation. Manalagi apples are fruits that are very easy to find, the fruit peel contains antibacterial compounds derived from polyphenols including catechins, quercetin, phloridzin and chlorogenic acid. The use of apple manalagi peel extract can be used as an antibacterial natural product especially in the oral cavity. The purpose of this study was to assess the inhibition of Manalagi apple peel extract (Malus sylvestris (L.) Mill) against the growth of Streptococcus sanguinis.
Method: This type of research is experimental with Post Test Only Control Group Design. There are 5 treatment groups, extract concentration of 25%, 50%, 100%, positive control with chlorhexidine 0.2%, and negative control with aquades. The antibacterial test method uses the disc diffusion method by saturating each extract into disc paper which then diffuses into the media to contain Streptococcus sanguinis bacteria.
Result: Phytochemical test results of manalagi apple peel extract showed saponin, fenol, terpenoid, flavonoid and alkaloid. The antibacterial activity test result showed of zone diameter at a concentration of 25% is 0 mm, a concentration of 50% 7.3 mm, a concentration of 100% 7.5 mm, positive control 14.3 mm and a negative control 0 mm. Data analysis used the Kruskal Wallis tests and showed p< 0,05.
Conclusion: The conclusion of this study is manalagi apple peel extract at a concentration of 50% and 100% has an antibacterial effect on the growth of Streptococcus sanguinis.
Latar Belakang: Streptococcus sanguinis merupakan salah satu bakteri gram positif penyebab penyakit gigi dan mulut yang berperan dalam proses pembentukan plak gigi. Buah apel manalagi adalah buah yang sangat mudah ditemukan, kulit buahnya mengandung senyawa antibakteri turunan polifenol diantaranya katekin, kuersetin, phloridzin, serta asam klorogenik. Penggunaan ekstrak kulit apel manalagi dapat digunakan sebagai produk alami antibakteri terutama dalam rongga mulut. Penelitian ini bertujuan guna menilai daya hambat ekstrak kulit apelmanalagi (Malus sylvestris (L.) Mill) terhadap pertumbuhan Streptococcus sanguinis.
Metode: Jenis penelitian eksperimental, dengan Post Test Only Control Group Design. Terdapat 5 kelompok perlakuan yaitu, konsentrasi ekstrak 25%,50%,100%, kontrol positif dengan chlorhexidine 0,2% serta kontrol negatif dengan aquades. Metode uji antibakteri menggunakan metode difusi cakram dengan menjenuhkan masing-masing ekstrak ke dalam kertas cakram yang kemudian berdifusi ke media agar yang berisi bakteri Streptococcus sanguinis.
Hasil: Uji fitokimia ekstrak kulit apel manalagi memperlihatkan adanya senyawa saponin, fenol, terpenoid, flavonoid, dan alkaloid. Hasil rerata diameter zona hambat pada konsentrasi 25% ialah 0 mm, konsentrasi 50% 7,3 mm, konsentrasi 100% 7,5 mm, kontrol positif 14,3 mm dan kontrol negatif 0 mm. Analisis data menggunakan uji Kruskal Wallis menunjukkan nilai p<0,05.
Kesimpulan: Ekstrak kulit apel manalagi pada konsentrasi 50% dan 100% memiliki daya antibakteri terhadap pertumbuhan Streptococcus sanguinis.
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Liu, Yu-Ting, Chin-Fu Lin, and Ya-Ling Lee. "Streptococcus sanguinis meningitis following endoscopic ligation for oesophageal variceal haemorrhage." Journal of Medical Microbiology 62, no. 5 (May 1, 2013): 794–96. http://dx.doi.org/10.1099/jmm.0.054213-0.
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We report a case of acute purulent meningitis caused by Streptococcus sanguinis after endoscopic ligation for oesophageal variceal haemorrhage in a cirrhotic patient without preceding symptoms of meningitis. Initial treatment with flomoxef failed. The patient was cured after 20 days of intravenous penicillin G. This uncommon infection due to S. sanguinis adds to the long list of infectious complications among patients with oesophageal variceal haemorrhage.
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TARRAGONA, Evelina L., Fernando S. FLORES, Candelaria L. HERRERA, Miriam DALINGER, Nerina AGUIRRE, Lucas D. MONJE, and Santiago NAVA. "Primer reporte de un caso de ehrlichiosis monocítica canina en la provincia de Santa Fe, Argentina." FAVE Sección Ciencias Veterinarias 18, no. 2 (November 28, 2019): 49–54. http://dx.doi.org/10.14409/favecv.v18i2.8438.
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La ehrlichiosis monocítica canina (EMC) es una enfermedad causada por la bacteria intracelular obligada Ehrlichia canis (Rickettsiales, Anaplasmataceae). La EMC es de importancia en salud animal por ser considerada de alta mortalidad en caninos domésticos, con distribución mundial; con síntomas inespecíficos como fiebre alta, depresión, letargia, anorexia y signos clínicos como linfoadenomegalia, esplenomegalia y trombocitopenia con tendencia hemorrágica, por lo que su diagnostico es un desafío en el entorno clínico. Ehrlichia canis es transmitida a un hospedador susceptible por ninfas y adultos de la garrapata Rhipicephalus sanguineus sensu lato. El taxón R. sanguineus s.l. representa un complejo de especies asociadas a caninos domésticos con distribución cosmopolita y particularmente en la región Neotropical se reconocen dos linajes: tropical y templado. El linaje templado, que representa a R. sanguineus sensu stricto, siendo el límite geográfico que separa ambos linajes una zona ecotonal situada entre 24 ° y 25 ° de latitud sur. En condiciones experimentales, R. sanguineus s.s. (linaje presente en la región centro de Argentina) demostró no tener competencia vectorial para transmitir E. canis (cepa Jaboticabal, Brasil) pero si R. sanguineus s.l. del linaje tropical. Sin embargo, estudios recientes han determinado molecularmente la infección con E. canis en muestras de sangre de perros con diagnóstico presuntivo de EMC en la provincia de Buenos Aires, dónde prevalece R. sanguineus s.s. Además, poblaciones de R. sanguineus s.l. (LT) y R. sanguineus s.s. de las provincias de Formosa y Buenos Aires, respectivamente, se detectaron naturalmente infectadas con E. canis. El presente informe de caso es el primer reporte de caso confirmado de EMC por E. canis en un canino de la ciudad de Rafaela, Santa Fe, área endémica de R. sanguineus s.s.
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Widyarman, Armelia Sari, Triska Ramajayanti, and Citra Fragrantia Theodorea. "Indonesian Strain of Lactobacillus reuteri Probiotic Reduces the Initial Biofilm Colonization." Open Dentistry Journal 14, no. 1 (November 13, 2020): 544–53. http://dx.doi.org/10.2174/1874210602014010544.
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Background: The benefits of probiotics for human health have long been proven. Probiotic Lactobacillus reuteri, can produce a beneficial broad-spectrum antibacterial compound called reuterin by metabolizing glycerol. Objective: The aim of the study was to investigate the effect of the Indonesian strain of L. reuteri LC382415 on mono- and dual-species Streptococcus mutans and Streptococcus sanguinis biofilms in vitro. Methods: Streptococcus mutans and S. sanguinis were cultured in BHI broth. Lactobacillus reuteri LC382415 was inoculated on MRS agar. The different concentrations effect of L. reuteri (1×104, 1×106, and 1×108 CFU/mL) with and without glycerol supplementation on microbial biofilms were examined using a biofilm assay after incubation for 1,3,6, and 24-h. The biofilm mass optical density was measured with a microplate spectrophotometer at 490 nm. Chlorhexidine gluconate (0.2%) was used as a positive control, and wells without treatment were used as negative controls. Results: A significant reduction in mono- and dual-species S. mutans and S. sanguinis biofilm formation was observed after treatment with all concentrations of L.reuteri and after all incubation periods (p<0.05) with or without glycerol supplementation. The concentration of 1×104 CFU/mL after 3-h incubation was the most effective in inhibiting biofilm formation, with 87.8% S. mutans, 95.9% S. sanguinis, and 80.4% dual-species biofilm reduction compared to the negative control (p<0.05). Conclusion: The Indonesian strain of L. reuteri effectively reduces mono- and dual-species S.mutans and S. sanguinis biofilms. This suggests that it may be useful in preventing biofilm formation in oral cavities. Future studies on the mechanism of action of this active component are warranted.
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Lan, Yiliang, Jie Yang, Xianbo Liu, Hanyu Zhao, Xinrui Zhang, Xueliang Yin, Chunguang Yang, Ke Yang, and Yi Liu. "Inhibition efficiency of 304-Cu stainless steel against oral bacterial biofilm." Journal of Applied Biomaterials & Functional Materials 20 (January 2022): 228080002110652. http://dx.doi.org/10.1177/22808000211065259.
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Purpose: This study aims to evaluate the antibacterial properties of 304 Cu-bearing stainless steel (SS) with different Cu contents (0, 2.5, 4.5 wt.%) against oral biofilms of Streptococcus mutans ( S. mutans), Streptococcus sanguinis ( S. sanguinis), and their mixture. Methods: Bacterial biofilms on the surface of 304-Cu SS were characterized by plate counting, 4′, 6-diamidino-2-phenylindole (DAPI) staining with aid of sanning electron microscopy (SEM) and 2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT). In addition, the inhibition zone method was also employed to evaluate the antibacterial properties of 304-Cu SS. Cell Counting Kit-8 (CCK-8) and flow cytometry were used to assess the cytotoxicity and apoptosis rate of 304-Cu SS, respectively. Results: 304-4.5Cu SS could effectively inhibit the attachment, formation, activity, and metabolism of bacterial biofilm, possessing the best antibacterial properties exceeding 99.9% of antibacterial rate against S. mutans, S. sanguinis, and their mixture. The diameters of inhibition zones to S. mutans and S. sanguinis on the surface of 304-4.5Cu SS were 21.7 and 14.7 mm, respectively. The results of cell experiments in vitro showed that both 304-2.5Cu SS and 304-4.5Cu SS had no evident cytotoxicity with an identical grade 1. The apoptosis rate exhibited a gradually increased tendency with increase of the Cu content in 304 SS. Conclusions: 304-4.5Cu SS without cytotoxic effect on NIH3T3 cells has obvious antibacterial activity against S. mutans, S. sanguinis and their mixture. Clinical significance: The Cu-bearing stainless steel provides a new solution to be used as oral orthodontic devices for inhibiting oral microflora imbalance and enamel demineralization.
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ADIWIJAYA, Wilma, Riani SETIADHI, and Vinna Kurniawati SUGIAMAN. "Antibacterial potential ethanol extract of beluntas leaves (Pluchea indica L) to Streptococcus sanguinis." Journal of Syiah Kuala Dentistry Society 7, no. 2 (January 11, 2023): 78–85. http://dx.doi.org/10.24815/jds.v7i2.30230.
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Recurrent aphthous stomatitis (RAS) is one of the most common oral diseases in the community, with a prevalence of 5-66%, with one of the predisposing factors being Streptococcus sanguinis. Treatment for RAS has been symptomatic and supportive, including antiseptic mouthwash such as chlorhexidine gluconate 0.2% or topical corticosteroids (triamcinolone acetonide 0.1% in Orabase). However, these drugs have some side effects. Treating herbal ingredients such as Beluntas leaves low prices and minimal side effects. The active compounds in Beluntas leaves are phenols, tannins, flavonoids, saponins, triterpenoids, essential oils, terpenoids, and many compounds known to have antibacterial activity. Methods: This study aimed to determine the minimum inhibitory level (MIC), and minimum killing rate (MBC) of 96% ethanol extract of Beluntas leaves on the growth of Streptococcus sanguinis. MIC was measured by broth microdilution technique with DMSO solvent 10% and eight concentrations of beluntas extract. Chlorhexidine gluconate 0.2% was used as a positive control for the comparison compound. Furthermore, the MBC test was carried out using the total plate count method for treatments that gave the MIC value. One Way Anova analysis with Post Hoc Tukey was used to determine the significant difference between treatments. Results: The ethanol extract of Beluntas leaves (Pluchea indica L) has a Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for the growth of Streptococcus sanguinis with a minimum inhibitory concentration of 3.95 g/mL and a minimum concentration of 7.8 g/mL. Conclusion: The ethanol extract of Beluntas leaves (Pluchea indica L) has the potential as an antibacterial against Streptococcus sanguinis. KEYWORDS: Recurrent aphthous stomatitis, Streptococcus sanguinis, ethanol extract of beluntas leaves, Minimum Inhibitory Concentration, Minimum Bactericidal Concentration
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Kemp, William P., and Norma E. Sánchez. "DIFFERENCES IN POST-DIAPAUSE THERMAL REQUIREMENTS FOR EGGS OF TWO RANGELAND GRASSHOPPERS." Canadian Entomologist 119, no. 7-8 (August 1987): 653–61. http://dx.doi.org/10.4039/ent119653-7.
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AbstractA study was conducted to examine the springtime hatching characteristics of Melanoplus sanguinipes (F.) and Aulocara elliotti (Thomas) eggs on rangeland. Laboratory experiments showed that eggs of M. sanguinipes required fewer accumulated degree days (DD) to complete post-diapause development than those of A. elliotti at a constant temperature of 23.3°C. Springtime field observations revealed that A. elliotti nymphs emerged prior to or concurrent with M. sanguinipes. Additionally, results suggest that A. elliotti nymphs emerge over a shorter interval than M. sanguinipes. Much of the variation in springtime emergence could be attributed to species-specific oviposition sites, pod depth, and pod orientation that resulted in A. elliotti eggs accumulating heat more rapidly than M. sanguinipes eggs. Results help explain some of the variation found in egg hatching of these two grasshopper species on rangeland.
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Zhu, Jianhui, Jiaxin Liu, Zhengyi Li, Ranhui Xi, Yuqing Li, Xian Peng, Xin Xu, Xin Zheng, and Xuedong Zhou. "The Effects of Nonnutritive Sweeteners on the Cariogenic Potential of Oral Microbiome." BioMed Research International 2021 (June 24, 2021): 1–10. http://dx.doi.org/10.1155/2021/9967035.
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Nonnutritive sweeteners (NNSs) are sugar substitutes widely used to reduce the negative health effects of excessive sugar consumption. Dental caries, one of the most prevalent chronic diseases globally, results from a pathogenic biofilm with microecological imbalance and frequent exposure to sugars. Some research has shown that certain NNSs possess less cariogenic potential than sucrose, indicating their putative effect on oral microbiome. To uncover the alterations of acidogenic pathogens and alkali-generating commensals, as well as the biofilm cariogenic potential under the influence of NNSs, we selected four common NNSs (acesulfame-K, aspartame, saccharin, and sucralose) and established single-, dual-, and multispecies in vitro culture model to assess their effects on Streptococcus mutans (S. mutans) and/or Streptococcus sanguinis (S. sanguinis) compared to sucrose with the same sweetness. The results showed that NNSs significantly suppressed the planktonic growth, acid production, and biofilm formation of S. mutans or S. sanguinis compared with sucrose in single-species cultures. Additionally, decreased S. mutans/S. sanguinis ratio, less EPS generation, and higher pH value were observed in dual-species and saliva-derived multispecies biofilms with supplementary NNSs. Collectively, this study demonstrates that NNSs inhibit the cariogenic potential of biofilms by maintaining microbial equilibrium, thus having a promising prospect as anticaries agents.
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Wijaya, Christofora Hanny, Bernadeta RE Sari, and Boy M. Bachtiar. "The potency of cajuputs candy in maintaining the competitive capacity of Streptococcus sanguinis upon Streptococcus mutans." Journal of Functional Food and Nutraceutical 1, no. 2 (February 29, 2020): 87–99. http://dx.doi.org/10.33555/jffn.v1i2.29.
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Streptococcus mutans were competing Streptococcus sanguinis in biofilm formation. As pioneer colonizer, S. sanguinis were able to control S. mutans growth. This study was aimed to explore the ability of sucrose and non-sucrose cajuputs candies (SCC and NSCC) in maintaining the antagonistic relationship between the indigenous oral flora when they grew as dual-species biofilms (S. sanguinis and S. mutans). The flavored candies (SCC and NSCC) contained cajuput and peppermint oils as the flavor which the volatile compounds had been identified. The unflavored candies were made similar to the flavored candy but excluding the flavor. The flavored candies, unflavored candies, and the control were exposed in vitro to the biofilms. The biofilms were examined for biofilm inhibition capacity, DNA amount, and the expression level of spxB mRNA. The biofilm inhibition by flavored candies were higher than the unflavored ones and were significantly different compared to the control. The flavored candies managed to decrease the total DNA amount in the biofilm, but unflavored samples did not. The qPCR assays showed that the exposure of candies did not alter the proportion of S. sanguinis DNA to S. mutans DNA in the biofilms. Meanwhile, spxB mRNA expression indicated the ability of S.sanguinis to control S. mutans growth.
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Sun, Yan, Yihuai Pan, Yu Sun, Mingyun Li, Shengbin Huang, Wei Qiu, Huanxin Tu, and Keke Zhang. "Effects of Norspermidine on Dual‐Species Biofilms Composed of Streptococcus mutans and Streptococcus sanguinis." BioMed Research International 2019 (November 3, 2019): 1–9. http://dx.doi.org/10.1155/2019/1950790.
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The present study aimed at investigating the influence of norspermidine on the formation of dual-species biofilms composed of Streptococcus mutans (S. mutans) and Streptococcus sanguinis (S. sanguinis). Crystal violet assay was conducted to assess the formation of single-species biofilms of S. mutans and S. sanguinis, and the growth curve was carefully observed to monitor the growth of these two species of bacteria. Fluorescence in situ hybridization (FISH) and MTT array were used to analyze the composition and metabolic activity of the dual-species biofilms, respectively. Extracellular polysaccharides (EPS)/bacteria staining, anthrone method, and scanning electron microscopy (SEM) imaging were conducted to study the synthesis of EPS by dual-species biofilms. Lactic acid assay and pH were measured to detect dual-species biofilm acid production. We found that norspermidine had different effects on S. mutans and S. sanguinis including their growth and biofilm formation. Norspermidine regulated the composition of the dual-species biofilms, decreased the ratio of S. mutans in dual-species biofilms, and reduced the metabolic activity, EPS synthesis, and acid production of dual-species biofilms. Norspermidine regulated dual-species biofilms in an ecological way, suggesting that it may be a potent reagent for controlling dental biofilms and managing dental caries.
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Cericatto Segalla, Catia, Natália Berne Pinheiro, Alexsander Ferraz, Taís Poletti, Renata Ongaratto, Cléber Martins Ribeiro, Tiago Felipe Barbosa Moreira, Pedro Spagnol, Marina Fucolo dos Santos, and Leandro Quintana Nizoli. "ESTUDO IN VITRO DA EFICÁCIA DO ÓLEO ESSENCIAL DE CRAVO-DA-ÍNDIA (Syzygium aromaticum, MYRTACEAE) SOBRE CARRAPATO Rhipicephalus sanguineus." Veterinária e Zootecnia 29 (September 27, 2022): 1–10. http://dx.doi.org/10.35172/rvz.2022.v29.889.
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Resumo: Rhipicephalus sanguineus vem se tornando uma praga urbana, com relatos de infestações em residências e populações resistentes a acaricidas, trazendo preocupações tanto a clínicos veterinários como também em saúde pública. Visando alternativas mais sustentáveis para o controle ambiental de R. sanguineus, o objetivo deste estudo foi avaliar o efeito in vitro do óleo essencial de cravo-da-índia, Syzygium aromaticum, sobre larvas de Rhipicephalus sanguineus. Esse óleo é rico em eugenol, composto aromático amplamente explorado pela indústria alimentícia e farmacêutica, e que apesar da sua excelente ação acaricida, existem poucos estudos de sua ação sobre R. sanguineus. Para realizar os testes foram utilizadas larvas com 15 dias de vida, sendo testadas as concentrações de 0,5%, 1% e 2% do óleo essencial. A sensibilidade das larvas foi testada pelos testes de imersão larval (TIL) com adaptações. Foi encontrada eficácia larvicida acima de 99% sobre R. sanguineus com concentrações acima de 0,5% de óleo de Syzygium aromaticum diluído em solução hidroalcóolica. Demonstrando promissor efeito acaricida do óleo essencial de cravo-da-índia sobre larvas de R. sanguineus.
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Andriko, Andriko, and Andrias Pujiono. "Korelasi Tipe Kepribadian Sanguin Terhadap Penginjilan." Skenoo : Jurnal Teologi dan Pendidikan Agama Kristen 2, no. 1 (June 26, 2022): 57–67. http://dx.doi.org/10.55649/skenoo.v2i1.28.
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Tipe Kepribadian adalah jenis karakteristik individu yang dapat menggambarkan respon dalam pikiran, perasaan dan tindakan. Setiap manusia memiliki tipe kepribadian unik, yang dalam fungsinya merupakan dasar dalam berperilaku. Dengan mengetahui tipe kepribadian jemaat diharapkan mampu diperoleh informasi siapa saja orang yang cenderung melakukan penginjilan. Untuk mengetahui hubungan tipe kepribadian sanguinis terhadap penginjilan maka dilakukan penelitian tentang kedua variabel tersebut. Dengan pendekatan kuantitatif, teknik pengambilan data melalui angket dengan skala Likert, dan pengolahan data menggunakan SPSS diperoleh angka korelasi 0,588. Berdasarkan tabel koefisien korelasi, angka tersebut termasuk dalam kategori sedang. Artinya, adanya korelasi yang cukup kuat antara variabel tipe kepribadian sanguinis (X) dan penginjilan (Y). Sedangkan untuk melihat berapa sumbangan variabel X terhadap Y, dilakukan uji Koefisien determinan dan mendapatkan angka sebesar 34,6%. Dari hasil tersebut, dapat disimpulkan bahwa tipe kepribadian sanguinis memiliki peranan penting dalam penginjilan.