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Journal articles on the topic 'SAMDI MS'

Author: Grafiati
Published: 9 March 2023
Last updated: 10 March 2023

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1

VanderPorten, Erica C., Michael D. Scholle, John Sherrill, John C. Tran, and Yichin Liu. "Identification of Small-Molecule Noncovalent Binders Utilizing SAMDI Technology." SLAS DISCOVERY: Advancing the Science of Drug Discovery 22, no. 10 (June 5, 2017): 1211–17. http://dx.doi.org/10.1177/2472555217712761.

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In recent years, the ability to unambiguously identify complex mixtures of analytes with high accuracy and resolving power in a label-free format continues to expand the application of mass spectrometry (MS) in the drug discovery process. This advantage combined with improved instrumentation makes MS suitable for targets with limited alternative assays for high-throughput screening (HTS). We describe a novel screening format using Self-Assembled Monolayers and matrix-assisted laser Desorption Ionization (SAMDI) technology. SAMDI enables affinity capture of a target protein for use in a small-molecule–binding assay format. Subsequent ionization enables the inferred identification of noncovalent compound interactions. SAMDI technology overcomes shot-to-shot variability by uniformly saturating the surface with captured protein, thereby minimizing matrix crystallization “hot spots.” Furthermore, the combination with high-resolution matrix-assisted laser desorption/ionization time of flight significantly reduces interference of small-molecule detection from salt, detergent, and matrix. By using a pooled library format, the SAMDI assay can significantly improve the throughput of MS-based screening irrespective of enzyme activity. Finally, we demonstrate binding affinity rank ordering from a pool of compounds that correlates with potency data from a biochemical assay.
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2

Scholle, Michael D., Doug McLaughlin, and Zachary A. Gurard-Levin. "High-Throughput Affinity Selection Mass Spectrometry Using SAMDI-MS to Identify Small-Molecule Binders of the Human Rhinovirus 3C Protease." SLAS DISCOVERY: Advancing the Science of Drug Discovery 26, no. 8 (June 19, 2021): 974–83. http://dx.doi.org/10.1177/24725552211023211.

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Affinity selection mass spectrometry (ASMS) has emerged as a powerful high-throughput screening tool used in drug discovery to identify novel ligands against therapeutic targets. This report describes the first high-throughput screen using a novel self-assembled monolayer desorption ionization (SAMDI)–ASMS methodology to reveal ligands for the human rhinovirus 3C (HRV3C) protease. The approach combines self-assembled monolayers of alkanethiolates on gold with matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) mass spectrometry (MS), a technique termed SAMDI-ASMS. The primary screen of more than 100,000 compounds in pools of 8 compounds per well was completed in less than 8 h, and informs on the binding potential and selectivity of each compound. Initial hits were confirmed in follow-up SAMDI-ASMS experiments in single-concentration and dose–response curves. The ligands identified by SAMDI-ASMS were further validated using differential scanning fluorimetry (DSF) and in functional protease assays against HRV3C and the related SARS-CoV-2 3CLpro enzyme. SAMDI-ASMS offers key benefits for drug discovery over traditional ASMS approaches, including the high-throughput workflow and readout, minimizing compound misbehavior by using smaller compound pools, and up to a 50-fold reduction in reagent consumption. The flexibility of this novel technology opens avenues for high-throughput ASMS assays of any target, thereby accelerating drug discovery for diverse diseases.
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3

Patel, Kaushal, John Sherrill, Milan Mrksich, and Michael D. Scholle. "Discovery of SIRT3 Inhibitors Using SAMDI Mass Spectrometry." Journal of Biomolecular Screening 20, no. 7 (May 29, 2015): 842–48. http://dx.doi.org/10.1177/1087057115588512.

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Lysine acetylation plays a critical role in cellular regulation and is implicated in human disease. Sirtuin deacetylases remove acetyl groups from modified lysine residues, and sirtuin 3 (SIRT3) has been identified as a target for cancer therapeutics. Robust and high-throughput screening methods for these targets will be important to the development of therapeutics. This article describes the use of self-assembled monolayer desorption/ionization mass spectrometry, or SAMDI-MS—a label-free drug discovery tool—to characterize SIRT3 activity and discover inhibitors. SAMDI-MS was used to analyze a peptide array having 361 distinct acetylated peptides to identify an active SIRT3 substrate (GYKAcRGC). This peptide was used in a screen of 100,000 small molecules to identify inhibitors of SIRT3. A total of 306 SIRT3 inhibitors were identified, with one compound, SDX-437, having an IC50 of 700 nM with >100-fold selectivity for SIRT3 over SIRT1.
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4

Grant, Jennifer, Patrick T. O’Kane, Blaise R. Kimmel, and Milan Mrksich. "Using Microfluidics and Imaging SAMDI-MS To Characterize Reaction Kinetics." ACS Central Science 5, no. 3 (February 12, 2019): 486–93. http://dx.doi.org/10.1021/acscentsci.8b00867.

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5

Techner, José-Marc, Weston Kightlinger, Liang Lin, Jasmine Hershewe, Ashvita Ramesh, Matthew P. DeLisa, Michael C. Jewett, and Milan Mrksich. "High-Throughput Synthesis and Analysis of Intact Glycoproteins Using SAMDI-MS." Analytical Chemistry 92, no. 2 (December 19, 2019): 1963–71. http://dx.doi.org/10.1021/acs.analchem.9b04334.

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6

Bayly, Alison A., Benjamin R. McDonald, Milan Mrksich, and Karl A. Scheidt. "High-throughput photocapture approach for reaction discovery." Proceedings of the National Academy of Sciences 117, no. 24 (June 1, 2020): 13261–66. http://dx.doi.org/10.1073/pnas.2003347117.

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Modern organic reaction discovery and development relies on the rapid assessment of large arrays of hypothesis-driven experiments. The time-intensive nature of reaction analysis presents the greatest practical barrier for the execution of this iterative process that underpins the development of new bioactive agents. Toward addressing this critical bottleneck, we report herein a high-throughput analysis (HTA) method of reaction mixtures by photocapture on a 384-spot diazirine-terminated self-assembled monolayer, and self-assembled monolayers for matrix-assisted laser desorption/ionization mass spectrometry (SAMDI-MS) analysis. This analytical platform has been applied to the identification of a single-electron-promoted reductive coupling of acyl azolium species.
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7

O’Kane, Patrick T., Quentin M. Dudley, Aislinn K. McMillan, Michael C. Jewett, and Milan Mrksich. "High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA." Science Advances 5, no. 6 (June 2019): eaaw9180. http://dx.doi.org/10.1126/sciadv.aaw9180.

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Metabolic engineering uses enzymes to produce small molecules with industrial, pharmaceutical, and energy applications. However, efforts to optimize enzymatic pathways for commercial production are limited by the throughput of assays for quantifying metabolic intermediates and end products. We developed a multiplexed method for profiling CoA-dependent pathways that uses a cysteine-terminated peptide to covalently capture CoA-bound metabolites. Captured metabolites are then rapidly separated from the complex mixture by immobilization onto arrays of self-assembled monolayers and directly quantified by SAMDI mass spectrometry. We demonstrate the throughput of the assay by characterizing the cell-free synthesis of HMG-CoA, a key intermediate in the biosynthesis of isoprenoids, collecting over 10,000 individual spectra to map more than 800 unique reaction conditions. We anticipate that our rapid and robust analytical method will accelerate efforts to engineer metabolic pathways.
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8

Scholle, Michael D., Patrick T. O'Kane, Sandra Dib, and Zachary A. Gurard-Levin. "Label-free duplex SAMDI-MS screen reveals novel SARS-CoV-2 3CLpro inhibitors." Antiviral Research 200 (April 2022): 105279. http://dx.doi.org/10.1016/j.antiviral.2022.105279.

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9

Roth, Michael J., Jaekuk Kim, Erica M. Maresh, Daniel A. Plymire, John R. Corbett, Junmei Zhang, and Steven M. Patrie. "Thin-Layer Matrix Sublimation with Vapor-Sorption Induced Co-Crystallization for Sensitive and Reproducible SAMDI-TOF MS Analysis of Protein Biosensors." Journal of The American Society for Mass Spectrometry 23, no. 10 (July 31, 2012): 1661–69. http://dx.doi.org/10.1007/s13361-012-0442-7.

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10

Wu, Ching-Yi, Kai-Chieh Lee, Yen-Ling Kuo, and Yu-Chie Chen. "Revisiting the quantitative features of surface-assisted laser desorption/ionization mass spectrometric analysis." Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 374, no. 2079 (October 28, 2016): 20150379. http://dx.doi.org/10.1098/rsta.2015.0379.

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Surface-assisted laser desorption/ionization (SALDI) coupled with mass spectrometry (MS) is frequently used to analyse small organics owing to its clean background. Inorganic materials can be used as energy absorbers and the transfer medium to facilitate the desorption/ionization of analytes; thus, they are used as SALDI-assisting materials. Many studies have demonstrated the usefulness of SALDI-MS in quantitative analysis of small organics. However, some characteristics occurring in SALDI-MS require certain attention to ensure the reliability of the quantitative analysis results. The appearance of a coffee-ring effect in SALDI sample preparation is the primary factor that can affect quantitative SALDI-MS analysis results. However, to the best of our knowledge, there are no reports relating to quantitative SALDI-MS analysis that discuss or consider this effect. In this study, the coffee-ring effect is discussed using nanoparticles and nanostructured substrates as SALDI-assisting materials to show how this effect influences SALDI-MS analysis results. Potential solutions for overcoming the existing problems are also suggested. This article is part of the themed issue ‘Quantitative mass spectrometry’.
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11

Szulc, Justyna, Artur Kołodziej, and Tomasz Ruman. "Silver-109/Silver/Gold Nanoparticle-Enhanced Target Surface-Assisted Laser Desorption/Ionisation Mass Spectrometry—The New Methods for an Assessment of Mycotoxin Concentration on Building Materials." Toxins 13, no. 1 (January 9, 2021): 45. http://dx.doi.org/10.3390/toxins13010045.

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This study aimed to detect and quantify mycotoxins on building materials using innovative laser mass spectroscopy methods—silver-109/silver/gold nanoparticle-enhanced target surface-assisted laser desorption/ionisation mass spectrometry (109AgNPs, AgNPs and AuNPs SALDI). Results from SALDI-type methods were also compared with commonly used matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. Standards of seven moulds mycotoxin in a final concentration of 100 µg/mL for patulin, citrinin, 3-nitropropionic acid, alternariol and 20 µg/mL for sterigmatocystin, cyclopiazonic acid, roquefortine C in the mixture were tested in pure solutions and after extraction from the plasterboards. Among the studied SALDI-type method, the lowest detection limits and the highest signal intensity of the mycotoxins tested were obtained with the use of 109AgNPs SALDI MS. The 109AgNPs method may be considered as an alternative to the currently most frequently used method MALDI MS and also liquid chromatography tandem mass spectrometry LC-MS/MS for mycotoxin determination. Future studies should attempt to use these methods for mass spectrometry imaging (MSI) to evaluate spatial distribution and depth of mycotoxin penetration into building materials.
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12

Szulc, Justyna, Artur Kołodziej, and Tomasz Ruman. "Silver-109/Silver/Gold Nanoparticle-Enhanced Target Surface-Assisted Laser Desorption/Ionisation Mass Spectrometry—The New Methods for an Assessment of Mycotoxin Concentration on Building Materials." Toxins 13, no. 1 (January 9, 2021): 45. http://dx.doi.org/10.3390/toxins13010045.

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This study aimed to detect and quantify mycotoxins on building materials using innovative laser mass spectroscopy methods—silver-109/silver/gold nanoparticle-enhanced target surface-assisted laser desorption/ionisation mass spectrometry (109AgNPs, AgNPs and AuNPs SALDI). Results from SALDI-type methods were also compared with commonly used matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. Standards of seven moulds mycotoxin in a final concentration of 100 µg/mL for patulin, citrinin, 3-nitropropionic acid, alternariol and 20 µg/mL for sterigmatocystin, cyclopiazonic acid, roquefortine C in the mixture were tested in pure solutions and after extraction from the plasterboards. Among the studied SALDI-type method, the lowest detection limits and the highest signal intensity of the mycotoxins tested were obtained with the use of 109AgNPs SALDI MS. The 109AgNPs method may be considered as an alternative to the currently most frequently used method MALDI MS and also liquid chromatography tandem mass spectrometry LC-MS/MS for mycotoxin determination. Future studies should attempt to use these methods for mass spectrometry imaging (MSI) to evaluate spatial distribution and depth of mycotoxin penetration into building materials.
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13

Liu, Chang, Lin, Liou, and Kuo. "High-Performance Sample Substrate of Gold Nanoparticle Multilayers for Surface-Assisted Laser Desorption/Ionization Mass Spectrometry." Nanomaterials 9, no. 8 (July 27, 2019): 1078. http://dx.doi.org/10.3390/nano9081078.

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The development of a sample substrate with superior performance for desorption and ionization of analyte is the key issue to ameliorate the quality of mass spectra for measurements of small molecules in surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Herein, the homogeneous sample substrate of gold nanoparticle multilayers (AuNPs-ML) with hexagonal lattice was successfully prepared by self-assembly technique. With strong surface plasmon resonance absorption and superior photothermal effect, the sample substrate of AuNPs-ML exhibited high signal sensitivity and low background noise for the detection of model analyte of glucose without additional matrixes in SALDI-MS. Furthermore, compared to merchant matrixes of α-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB), the sample substrate of AuNPs-ML was demonstrated to ameliorate the quality of mass spectra, including signal strength, background interference and signal/noise (S/N) ratio. The sucrose and tryptophan were also measured to show the extensive applications of AuNPs-ML sample substrate for the detections of small molecules in SALDI-MS. Most importantly, the remarkable reproducibility of glucose mass spectra with relative signal of 7.3% was obtained by the use of AuNPs-ML sample substrate for SALDI-MS. The homogeneous sample substrate of AuNPs-ML greatly improved the quality of mass spectra because of its strong absorption of laser energy, low specific heat, high heat conductivity and extraordinary homogeneity. We believe that AuNPs-ML could be a practical sample substrate for small molecule detection in SALDI-MS.
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14

Gorbunov, A. Yu, I. M. Zorin, S. K. Ilyushonok, A. A. Bardin, O. A. Keltsieva, N. V. Krasnov, V. N. Babakov, and E. P. Podolskaya. "Application of MALDI target electrophoretically modified with TiO2 for mass spectrometry with surface-assisted laser desorpion / ionization." NAUCHNOE PRIBOROSTROENIE 31, no. 1 (February 19, 2021): 44–58. http://dx.doi.org/10.18358/np-31-1-i4458.

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In this investigation, parameters of electrophoretic deposition (EPO) of TiO2 nanoparticles on stainless steel substrate have been optimized. The obtained coating was used as ion emitter during surface-assisted laser desorpion/ionization (SALDI). Herein, we demonstrate the high efficiency of obtained coatings for SALDI of amiodarone with subsequent Fourier transform ion cyclotron resonance mass spectrometry. Additional modification of coatings with polydimethylsiloxane (PDMS) allowed to significantly improve the sensitivity of SALDI-MS analysis.
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15

Huang, Yu-Hui, Chia-Wei Wang, Wen-Tsen Chen, Li-Yi Chen, and Huan-Tsung Chang. "Nanomaterial based mass spectrometry of oligodeoxynucleotide–drug complexes." Analytical Methods 7, no. 15 (2015): 6360–64. http://dx.doi.org/10.1039/c5ay00990a.

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16

Tsao, Chia-Wen, Yuan-Jing Lin, Pi-Yu Chen, Yu-Liang Yang, and Say Hwa Tan. "Nanoscale silicon surface-assisted laser desorption/ionization mass spectrometry: environment stability and activation by simple vacuum oven desiccation." Analyst 141, no. 16 (2016): 4973–81. http://dx.doi.org/10.1039/c6an00659k.

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17

Krishnan, Sanduru Thamarai, David Rudd, Rana Rahmani, E. Eduardo Antunez, Rajpreet Singh Minhas, Chandra Kirana, Guy J. Maddern, Kevin Fenix, Ehud Hauben, and Nicolas H. Voelcker. "Nanostructured Silicon Enabled HR-MS for the Label-Free Detection of Biomarkers in Colorectal Cancer Plasma Small Extracellular Vesicles." Journal of Nanotheranostics 3, no. 4 (October 4, 2022): 189–202. http://dx.doi.org/10.3390/jnt3040013.

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Despite improvements in treatment options for advanced colorectal cancer (CRC), survival outcomes are still best for patients with non-metastasised disease. Diagnostic tools to identify blood-based biomarkers and assist in CRC subtype classification could afford a means to track CRC progression and treatment response. Cancer cell-derived small extracellular vesicles (EVs) circulating in blood carry an elevated cargo of lipids and proteins that could be used as a signature of tumour suppressor/promoting events or stages leading up to and including metastasis. Here, we used pre-characterised biobanked plasma samples from surgical units, typically with a low volume (~100 µL), to generate and discover signatures of CRC-derived EVs. We employed nanostructured porous silicon (pSi) surface assisted-laser desorption/ionisation (SALDI) coupled with high-resolution mass spectrometry (HR-MS), to allow sensitive detection of low abundant analytes in plasma EVs. When applied to CRC samples, SALDI-HR-MS enabled the detection of the peptide mass fingerprint of cancer suppressor proteins, including serine/threonine phosphatases and activating-transcription factor 3. SALDI-HR-MS also allowed the detection of a spectrum of glycerophospholipids and sphingolipid signatures in metastatic CRC. We observed that lithium chloride enhanced detection sensitivity to elucidate the structure of low abundant lipids in plasma EVs. pSi SALDI can be used as an effective system for label-free and high throughput analysis of low-volume patient samples, allowing rapid and sensitive analysis for CRC classification.
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18

Kuo, Tsung-Rong, Yin-Chien Chen, Chiung-I. Wang, Tzu-Hau Shen, Hong-Yi Wang, Xi-Yu Pan, Di-Yan Wang, et al. "Highly oriented Langmuir–Blodgett film of silver cuboctahedra as an effective matrix-free sample plate for surface-assisted laser desorption/ionization mass spectrometry." Nanoscale 9, no. 31 (2017): 11119–25. http://dx.doi.org/10.1039/c7nr04098a.

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19

Niu, Hongyun, Saihua Wang, Yixin Tan, Xiaowei Song, and Yaqi Cai. "Simultaneous and direct analysis of multiple types of organic contaminants in water based on a MOF decorated with a suitable quantity of Au nanoparticles, using SALDI-TOF MS." RSC Advances 6, no. 102 (2016): 99919–23. http://dx.doi.org/10.1039/c6ra19635g.

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20

Li, Yafeng, Peiqi Luo, Xiaohua Cao, Huihui Liu, Jianing Wang, Jiyun Wang, Lingpeng Zhan, and Zongxiu Nie. "Enhancing surface-assisted laser desorption ionization mass spectrometry performance by integrating plasmonic hot-electron transfer effect through surface modification." Chemical Communications 55, no. 41 (2019): 5769–72. http://dx.doi.org/10.1039/c9cc02541c.

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21

Ma, Wen, Shuting Xu, Wanpeng Ai, Cheng Lin, Yu Bai, and Huwei Liu. "A flexible and multifunctional metal–organic framework as a matrix for analysis of small molecules using laser desorption/ionization mass spectrometry." Chemical Communications 55, no. 48 (2019): 6898–901. http://dx.doi.org/10.1039/c9cc02611h.

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22

Abdelhamid, Hani Nasser, and Hui-Fen Wu. "Synthesis of a highly dispersive sinapinic acid@graphene oxide (SA@GO) and its applications as a novel surface assisted laser desorption/ionization mass spectrometry for proteomics and pathogenic bacteria biosensing." Analyst 140, no. 5 (2015): 1555–65. http://dx.doi.org/10.1039/c4an02158d.

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23

Lee, Gwangbin, Sang-Eun Bae, Seong Huh, and Sangwon Cha. "Graphene oxide embedded sol–gel (GOSG) film as a SALDI MS substrate for robust metabolite fingerprinting." RSC Advances 5, no. 70 (2015): 56455–59. http://dx.doi.org/10.1039/c5ra11497g.

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24

Al-Sayed, Sara A., Mohamed O. Amin, and Entesar Al-Hetlani. "SALDI Substrate-Based FeNi Magnetic Alloy Nanoparticles for Forensic Analysis of Poisons in Human Serum." Molecules 27, no. 9 (April 23, 2022): 2720. http://dx.doi.org/10.3390/molecules27092720.

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In this study, FeNi magnetic alloy nanoparticles (MANPs) were employed for the forensic analysis of four poisons—dimethametryn, napropamide, thiodicarb, and strychnine—using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). FeNi MANPs were prepared via coprecipitation using two reducing agents, sodium borohydride (NaBH4) and hydrazine monohydrate (N2H4·H2O), to optimize the prepared MANPs and investigate their effect on the performance of SALDI-MS analysis. Thereafter, SALDI-MS analysis was carried out for the detection of three pesticides and a rodenticide. The prepared substrate offered sensitive detection of the targeted analytes with LOD values of 1 ng/mL, 100 pg/mL, 10 ng/mL, and 200 ng/mL for dimethametryn, napropamide, thiodicarb, and strychnine, respectively. The relative standard deviation (%RSD) values were in the range of 2.30–13.97% for the pesticides and 15–23.81% for strychnine, demonstrating the good spot-to-spot reproducibility of the FeNi substrate. Finally, the MANPs were successfully employed in the analysis of poison-spiked blood serum using a minute quantity of the sample with an LOD of 700 ng/mL dimethametryn and napropamide, 800 ng/mL thiodicarb, and 500 ng/mL strychnine. This study has great potential regarding the analysis of several poisons that may be found in human serum, which is significant in cases of self-harm.
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25

Ohta, Takayuki, Hironori Ito, Kenji Ishikawa, Hiroki Kondo, Mineo Hiramatsu, and Masaru Hori. "Atmospheric Pressure Plasma-Treated Carbon Nanowalls’ Surface-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (CNW-SALDI-MS)." C 5, no. 3 (July 18, 2019): 40. http://dx.doi.org/10.3390/c5030040.

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Carbon nanowalls (CNWs), vertically standing highly crystallizing graphene sheets, were used in the application of a surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF-MS). The CNW substrates solved the issues on interferences of matrix molecules and alkali metal addition ions in low-weight molecule detection. Before SALDI sample preparations, the hydrophobic CNW was treated by atmospheric pressure plasma for exposing hydrophilicity to the CNWs’ surface. Detection of water soluble amino acids, arginine, was demonstrated.
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26

Guinan, T. M., P. Kirkbride, C. B. Della Vedova, S. G. Kershaw, H. Kobus, and N. H. Voelcker. "Direct detection of illicit drugs from biological fluids by desorption/ionization mass spectrometry with nanoporous silicon microparticles." Analyst 140, no. 23 (2015): 7926–33. http://dx.doi.org/10.1039/c5an01754h.

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Surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) with porous silicon microparticles was used for the all-in-one extraction and detection of illicit drugs from saliva, urine and plasma.
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27

Silina, Yuliya E., Claudia Fink-Straube, Heiko Hayen, and Dietrich A. Volmer. "Analysis of fatty acids and triacylglycerides by Pd nanoparticle-assisted laser desorption/ionization mass spectrometry." Analytical Methods 7, no. 9 (2015): 3701–7. http://dx.doi.org/10.1039/c5ay00705d.

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In this study, we propose a simple and rapid technique for characterization of free fatty acids and triacylglycerides (TAG) based on palladium nanoparticular (Pd-NP) surface-assisted laser desorption/ionization (SALDI) mass spectrometry (MS).
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28

Li, Ze, Yi-Wei Zhang, Yue-Long Xin, Yu Bai, Heng-Hui Zhou, and Hu-Wei Liu. "A lithium-rich composite metal oxide used as a SALDI-MS matrix for the determination of small biomolecules." Chem. Commun. 50, no. 97 (2014): 15397–99. http://dx.doi.org/10.1039/c4cc07479c.

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29

Al-Rajeh, S., A. Awada, and O. Bademosi. "MS in Saudi Arabia." Neurology 39, no. 2 (February 1, 1989): 308. http://dx.doi.org/10.1212/wnl.39.2.308-b.

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30

Yaqub, B. A., and A. K. Daif. "MS in Saudi Arabia." Neurology 39, no. 2 (February 1, 1989): 308. http://dx.doi.org/10.1212/wnl.39.2.308-c.

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31

Zhang, Yanhao, Yuanyuan Song, Jie Wu, Ruijin Li, Di Hu, Zian Lin, and Zongwei Cai. "A magnetic covalent organic framework as an adsorbent and a new matrix for enrichment and rapid determination of PAHs and their derivatives in PM2.5 by surface-assisted laser desorption/ionization-time of flight-mass spectrometry." Chemical Communications 55, no. 26 (2019): 3745–48. http://dx.doi.org/10.1039/c9cc00384c.

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Fe3O4@COFs served as an adsorbent and new matrix for SALDI-TOF-MS analysis of PAHs and their derivatives in PM2.5 with clear background, good reproducibility and sensitivity.
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32

Sakai, Ryusei, Hiroki Kondo, Kenji Ishikawa, Takayuki Ohta, Mineo Hiramatsu, Hiromasa Tanaka, and Masaru Hori. "Effects of High-Quality Carbon Nanowalls Ionization-Assisting Substrates on Surface-Assisted Laser Desorption/Ionization Mass Spectrometry Performance." Nanomaterials 13, no. 1 (December 23, 2022): 63. http://dx.doi.org/10.3390/nano13010063.

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Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is performed using carbon nanowalls (CNWs) for ionization-assisting substrates. The CNWs (referred to as high-quality CNWs) in the present study were grown using a radical-injection plasma-enhanced chemical vapor deposition (RI-PECVD) system with the addition of oxygen in a mixture of CH4 and H2 gases. High-quality CNWs were different with respect to crystallinity and C–OH groups, while showing similar wall-to-wall distances and a wettability comparable to CNWs (referred to as normal CNWs) grown without O2. The efficiency of SALDI was tested with both parameters of ion intensity and fragmental efficiency (survival yield (SY)) using N-benzylpyridinuim chloride (N-BP-CI). At a laser fluence of 4 mJ/cm2, normal CNWs had an SY of 0.97 and an ion intensity of 0.13, while 5-sccm-O2– high-quality CNWs had an SY of 0.89 and an ion intensity of 2.55. As a result, the sensitivity for the detection of low-molecular-weight analytes was improved with the high-quality CNWs compared to the normal CNWs, while an SY of 0.89 was maintained at a low laser fluence of 4 mJ/cm2. SALDI-MS measurements available with the high-quality CNWs ionization-assisting substrate provided high ionization and SY values.
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33

Yang, Tzu-Ling, Cheng-Liang Huang, and Chu-Ping Lee. "Utilizing AgNPt-SALDI to Classify Edible Oils by Multivariate Statistics of Triacylglycerol Profile." Molecules 26, no. 19 (September 28, 2021): 5880. http://dx.doi.org/10.3390/molecules26195880.

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Edible oils are valuable sources of nutrients, and their classification is necessary to ensure high quality, which is essential to food safety. This study reports the establishment of a rapid and straightforward SALDI-TOF MS platform used to detect triacylglycerol (TAG) in various edible oils. Silver nanoplates (AgNPts) were used to optimize the SALDI samples for high sensitivity and reproducibility of TAG signals. TAG fingerprints were combined with multivariate statistics to identify the critical features of edible oil discrimination. Eleven various edible oils were discriminated using principal component analysis (PCA). The results suggested the creation of a robust platform that can examine food adulteration and food fraud, potentially ensuring high-quality foods and agricultural products.
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Roverso, Marco, Roberta Seraglia, Raghav Dogra, Denis Badocco, Silvia Pettenuzzo, Luca Cappellin, Paolo Pastore, and Sara Bogialli. "Single-Walled Carbon Nanohorns as Boosting Surface for the Analysis of Low-Molecular-Weight Compounds by SALDI-MS." International Journal of Molecular Sciences 23, no. 9 (April 30, 2022): 5027. http://dx.doi.org/10.3390/ijms23095027.

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Limits of Matrix-Assisted Laser Desorption Ionization (MALDI) mass spectrometry (MS) in the study of small molecules are due to matrix-related interfering species in the low m/z range. Single-walled carbon nanohorns (SWCNH) were here evaluated as a specific surface for the rapid analysis of amino acids and lipids by Surface-Assisted Laser Desorption Ionization (SALDI). The method was optimized for detecting twenty amino acids, mainly present as cationized species, with the [M+K]+ response generally 2-time larger than the [M+Na]+ one. The [M+Na]+/[M+K]+ signals ratio was tentatively correlated with the molecular weight, dipole moment and binding affinity, to describe the amino acids’ coordination ability. The SWCNH-based surface was also tested for analyzing triglycerides in olive oil samples, showing promising results in determining the percentage composition of fatty acids without any sample treatment. Results indicated that SWCNH is a promising substrate for the SALDI-MS analysis of low molecular weight compounds with different polarities, enlarging the analytical platforms for MALDI applications.
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35

Jumah, M. A. A. "Genetics of MS in Saudi Arabia." Multiple Sclerosis and Related Disorders 3, no. 6 (November 2014): 743–44. http://dx.doi.org/10.1016/j.msard.2014.09.163.

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36

Sakai, Ryusei, Tomonori Ichikawa, Hiroki Kondo, Kenji Ishikawa, Naohiro Shimizu, Takayuki Ohta, Mineo Hiramatsu, and Masaru Hori. "Effects of Carbon Nanowalls (CNWs) Substrates on Soft Ionization of Low-Molecular-Weight Organic Compounds in Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS)." Nanomaterials 11, no. 2 (January 20, 2021): 262. http://dx.doi.org/10.3390/nano11020262.

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Carbon nanowalls (CNWs), which are vertically oriented multi-layer graphene sheets, were employed in surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) measurements to detect low-molecular-weight organic compounds. CNWs substrates with widely different wall-to-wall distances from 142 to 467 nm were synthesized using a radical-injection plasma-enhanced chemical vapor deposition (RI-PECVD) system with nanosecond pulse biasing to a sample stage. When survival yield (SY) values of N-benzylpyridinium chloride (N-BP-Cl) were examined, which is commonly used to evaluate desorption/ionization efficiency, a narrower wall-to-wall distance presented a higher SY value. The highest SY value of 0.97 was realized at 4 mJ/cm2 for the highest-density CNWs with a wall-to-wall distance of 142 nm. The laser desorption/ionization effect of arginine, an amino acid, was also investigated. When CNWs with a narrower wall-to-wall distance were used, the signal-to-noise (SN) ratios of the arginine signals were increased, while the intensity ratios of fragment ions to arginine signals were suppressed. Therefore, the CNWs nanostructures are a powerful tool when used as a SALDI substrate for the highly efficient desorption/ionization of low-molecular-weight biomolecules.
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37

Almutairi, Mohammed, Turki Alsaleem, Hatem Al Herbish, Abdullah A. Al Sayari, and Abdullah M. Alowaifeer. "LC-MS/MS and GC-MS/MS analysis of pesticide residues in Ecuadorian and Filipino Cavendish bananas imported into Saudi Arabia." Food Additives & Contaminants: Part A 38, no. 8 (June 2, 2021): 1376–85. http://dx.doi.org/10.1080/19440049.2021.1930199.

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38

Bunyan, Reem, Ghada Al Towaijri, Hessa Al Otaibi, Abid Kareem, Hussein Algahtani, Mousa Al Mejally, Ali Almubarak, et al. "Prevalence of Pediatric Onset Multiple Sclerosis in Saudi Arabia." Multiple Sclerosis International 2021 (November 9, 2021): 1–6. http://dx.doi.org/10.1155/2021/4226141.

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Background. The prevalence of multiple sclerosis (MS) appears to be increasing worldwide. However, data on the pediatric onset of MS is lacking, particularly in developing countries. Objective. This study is aimed at reporting the current burden of the pediatric onset of MS in the five regions of Saudi Arabia. Methods. This study used relevant data from the National Saudi MS Registry that was operational between 2015 and 2018. The data on patients with pediatric onset MS from all the hospitals included in the registry was retrospectively analyzed using the age of diagnosis. Patients who were 1-18 years old when diagnosed were included in the analysis. Results. The registry included 287 patients with pediatric onset MS, with a mean age of diagnosis at 15.7 (SD: 2.06). 74.2% of the participants were females. For the included hospitals, the estimated prevalence of pediatric MS was at 2.73/100,000 pediatric Saudi population. The prevalence of pediatric MS in the remaining nonparticipant hospitals was then projected taking into account both the size of pediatric population in the Kingdom per region and the number of facilities treating and managing MS in each of the corresponding regions. The overall projected prevalence was found to be 14.33/100,000 Saudi pediatric population. Conclusion. To the best of our knowledge, this study reported the latest epidemiological data of pediatric onset of MS in Saudi Arabia. The current prevalence of MS among the pediatric Saudi population was found to be 2.73/100,000, and the overall projected prevalence was estimated at 14.33/100,000. Our findings were similar to those in other pediatric MS cohorts. Further studies are needed to understand the long-term prognosis, response to treatment, and disease course.
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39

Al-Quadeib, Bushra T., Mahasen A. Radwan, Lidija Siller, Elaine Mutch, Ben Horrocks, Matthew Wright, and Abdulaziz Alshaer. "Therapeutic monitoring of amphotericin B in Saudi ICU patients using UPLC MS/MS assay." Biomedical Chromatography 28, no. 12 (April 14, 2014): 1652–59. http://dx.doi.org/10.1002/bmc.3198.

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40

Ababutain, Ibtisam. "Antimicrobial Activity and Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Saudi Arabian Ocimum basilicum Leaves Extracts." Journal of Pure and Applied Microbiology 13, no. 2 (June 30, 2019): 823–33. http://dx.doi.org/10.22207/jpam.13.2.17.

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41

Lim, Angelina Yimei, Jan Ma, and Yin Chiang Freddy Boey. "Development of Nanomaterials for SALDI-MS Analysis in Forensics." Advanced Materials 24, no. 30 (May 29, 2012): 4211–16. http://dx.doi.org/10.1002/adma.201200027.

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42

Murakami, Chiaki, Fumi Hoshino, Hiromichi Sakai, Yasuhiro Hayashi, Atsushi Yamashita, and Fumio Sakane. "Diacylglycerol kinase δ and sphingomyelin synthase–related protein functionally interact via their sterile α motif domains." Journal of Biological Chemistry 295, no. 10 (January 24, 2020): 2932–47. http://dx.doi.org/10.1074/jbc.ra119.012369.

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The δ isozyme of diacylglycerol kinase (DGKδ) plays critical roles in lipid signaling by converting diacylglycerol (DG) to phosphatidic acid (PA). We previously demonstrated that DGKδ preferably phosphorylates palmitic acid (16:0)- and/or palmitoleic acid (16:1)-containing DG molecular species, but not arachidonic acid (20:4)-containing DG species, which are recognized as DGK substrates derived from phosphatidylinositol turnover, in high glucose-stimulated myoblasts. However, little is known about the origin of these DG molecular species. DGKδ and two DG-generating enzymes, sphingomyelin synthase (SMS) 1 and SMS-related protein (SMSr), contain a sterile α motif domain (SAMD). In this study, we found that SMSr–SAMD, but not SMS1–SAMD, co-immunoprecipitates with DGKδ–SAMD. Full-length DGKδ co-precipitated with full-length SMSr more strongly than with SMS1. However, SAMD-deleted variants of SMSr and DGKδ interacted only weakly with full-length DGKδ and SMSr, respectively. These results strongly suggested that DGKδ interacts with SMSr through their respective SAMDs. To determine the functional outcomes of the relationship between DGKδ and SMSr, we used LC-MS/MS to investigate whether overexpression of DGKδ and/or SMSr in COS-7 cells alters the levels of PA species. We found that SMSr overexpression significantly enhances the production of 16:0- or 16:1-containing PA species such as 14:0/16:0-, 16:0/16:0-, 16:0/18:1-, and/or 16:1/18:1-PA in DGKδ-overexpressing COS-7 cells. Moreover, SMSr enhanced DGKδ activity via their SAMDs in vitro. Taken together, these results strongly suggest that SMSr is a candidate DG-providing enzyme upstream of DGKδ and that the two enzymes represent a new pathway independent of phosphatidylinositol turnover.
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43

Shariatgorji, Mohammadreza, Nahid Amini, Gunnar Thorsen, Carlo Crescenzi, and Leopold L. Ilag. "μ-Trap for the SALDI-MS Screening of Organic Compounds Prior to LC/MS Analysis." Analytical Chemistry 80, no. 14 (July 2008): 5515–23. http://dx.doi.org/10.1021/ac8005186.

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44

Alluqmani, M., M. Alqermli, G. Blevins, B. Alotibi, F. Giuliani, and P. Christopher. "Disease phenotype analyses of relapsing-remitting multiple sclerosis in Canada and Saudi Arabia." Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques 42, S1 (May 2015): S32. http://dx.doi.org/10.1017/cjn.2015.151.

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Background: Multiple sclerosis (MS) exhibits a spectrum of clinical findings, especially in relapsing-remitting MS (RR-MS). To assess the effects of geographic location and ethnicity on RR-MS phenotype, we investigated RR-MS patients in Canada and Saudi Arabia. Methods: A retrospective cross-sectional analysis of patients receiving active care in MS Clinics was performed in Medina, Saudi Arabia and Edmonton, Alberta. Demographic and clinical data was collected for each patient. Results: 98 patients with treated RR-MS were recruited (n=51, Medina; n=47, Edmonton); 40 patients were Caucasian (Edmonton) while 46 patients were Bedouin (Medina). Although the disease duration was longer in the Edmonton (5.7+2.3 yr) compared to the Medina group (4.4+1.4 yr) (p<0.05), the mean age of RR-MS onset, relapse rate and EDSS change were similar. The female:male ratio was comparable in Edmonton (35:12) and Medina (32:19), as was the risk of optic neuritis. The likelihood of an infratentorial lesion-associated presentation differed (Edmonton, n=23; Medina; n=13) among groups (p<0.05). Spinal cord lesions on MRI were more frequent in Edmonton (n=18) compared to Medina (n=1) patients (p<0.05). Conclusions: Despite differences in location, ethnicity, and a predominance of infratentorial lesion burden the Edmonton group, the RR-MS phenotype displayed similar disease severity and trajectory in these cohorts.
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Zhou, Di, Na Song, Shuzhen Dou, Jiaqi Liu, Qiye Chen, Xiaofeng Lu, and Nan Lu. "A flexible SALDI-MS substrate for no background interference detection." Sensors and Actuators B: Chemical 351 (January 2022): 130868. http://dx.doi.org/10.1016/j.snb.2021.130868.

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46

Amini, Nahid, Mohammadreza Shariatgorji, and Gunnar Thorsén. "SALDI-MS Signal enhancement using oxidized graphitized carbon black nanoparticles." Journal of the American Society for Mass Spectrometry 20, no. 6 (June 2009): 1207–13. http://dx.doi.org/10.1016/j.jasms.2009.02.017.

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47

Alshehrei, Fatimah. "Antimicrobial Activity and Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Saudi Arabian Ocimum basilicum Leaves Extracts." Journal of Pure and Applied Microbiology 13, no. 2 (June 30, 2019): 897–904. http://dx.doi.org/10.22207/jpam.13.2.26.

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48

Abdulsallam, Bakdash, Almohammadi Ohood Hasan, Taha Nael Abu, Abu-Rumman Ali, and Kumar Sachil. "Chemical composition of propolis from the Baha region in Saudi Arabia." Czech Journal of Food Sciences 36, No. 2 (May 7, 2018): 109–18. http://dx.doi.org/10.17221/119/2017-cjfs.

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The chemical composition of organic compounds in the extractable organic matter of propolis collected from the Baha region of Saudi Arabia was investigated. The propolis samples were extracted with methanol followed by column chromatography and collected in fractions (15 ml), then analysed by gas chromatography-mass spectrometry (GC-MS). Samples were injected with and without derivatization using BSTFA simultaneously. The results showed that a total of 61 chemical compounds were characterized and identified by analysing each of the fractions. Out of these, 33 compounds have not been reported previously. The Baha sample contains a high amount of aromatic acids, alcohol and phenol aldehydes. In addition to these, some other compounds (aliphatic acids, sugar derivatives, steroid derivatives and flavone derivatives) were also present. Some of the identified compounds have shown diverse biological activities: antimicrobial, anti-inflammatory, and analgesic activities. Methanolic extraction of propolis followed by column chromatography and GC-MS resulted in the separation of a high number of compounds compared with propolis samples from different geographical regions. The new identified compounds were found related to the flora of the Baha region. Therefore, the composition of propolis differs according to the plants found in the area in which it is produced. This may be used in the determination of a geographical area as the source of a propolis sample.
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49

Alqarni, Ibrahim R. "Saudi English Major Freshmen Students’ Vocabulary Learning Strategies: An Exploratory Study." International Journal of Applied Linguistics and English Literature 7, no. 1 (December 15, 2017): 141. http://dx.doi.org/10.7575/aiac.ijalel.v.7n.1p.141.

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This study aims at exploring the vocabulary learning strategies (VLSs) employed by Saudi Freshmen students majoring in English as a foreign language (EFL). The participants are 81 Saudi male students in their first semester in the English Department and Translation in the College of Language and Translation at King Saud University. Data was collected using a questionnaire which was adapted from the study of Rabadi (2016) and was analyzed using the SPSS program. The overall results of this study show that participants use all of the different vocabulary learning strategies: Determination strategies, Memory strategies, Cognitive strategies, Metacognitive strategies, and Social strategies, with different degrees of frequency. By looking at the sub-categories of the strategies the results indicate that Metacognitive strategies (mean score: 1.98/4) are the most used and/or preferred strategies by all participants, followed by Social strategies (MS: 1.91), Determination strategies (MS: 1.62), Cognitive strategies (MS: 1.39) and Memory strategies (MS: 1.26) respectively. However, the overall mean score of (1.63) for the use of the strategies indicates that the participants of this study are low/poor users of vocabulary learning strategies in general.
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50

Al-Kafaji, Ghada, Materah Salem Alwehaidah, Manahel Mahmood Alsabbagh, Maram A. Alharbi, and Moiz Bakhiet. "Mitochondrial DNA haplogroup analysis in Saudi Arab patients with multiple sclerosis." PLOS ONE 17, no. 12 (December 19, 2022): e0279237. http://dx.doi.org/10.1371/journal.pone.0279237.

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Previous studies have suggested that mitochondrial DNA (mtDNA) variants are associated with multiple sclerosis (MS), a complex neurodegenerative immune-mediated disease of the central nervous system. Since mtDNA is maternally inherited without recombination, specific mtDNA variants defining genetic background are associated with the susceptibility to human diseases. To assess the contribution of mtDNA haplogroups to the predisposition of MS in an Arab population, we analysed sequencing data of mitochondrial genomes from 47 native Saudi Arab individuals including 23 patients with relapsing-remitting MS (RRMS) and 24 healthy controls. All patients and controls could be classified into ten haplogroups. The European-specific haplogroup U was more prevalent in patients than in the controls (26.1% vs. 4.2%), whereas haplogroup T was only present in patients and haplogroups HV and N were only found in controls. Haplogroup U was significantly association with increased risk of MS (odds ratio = 6.26, p<0.05), although the association did not maintain significance after adjustment for multiple comparisons. Haplotype U was more prevalent in patients with younger age of onset (p = 0.006), but there was no relationship between haplotype U and disease severity, disease duration or EDSS and age-matched carriers and non-carriers of haplogroup U (p>0.05). Definition site of haplogroup U include the variant m.12308A>G in MT-TL2 gene which was found to affect highly conserved position within the variable arm of tRNALeu(CUN) and thus may impact mitochondrial protein synthesis, and two other variants namely m.11467A>G in MT-ND4 gene and m.12372G>A in MT-ND5 gene which were previously linked with mitochondrial function. Despite the small number of subjects, which may limit the statistical power of the study, our results showed for the first time a possible contribution of haplogroup U to the predisposition to MS in an Arab population. These findings warrant further validation in a large cohort to distinguish a genuine effect specific to MS from a chance finding due to small sampling.
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