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Journal articles on the topic 'Saccharomyces cerevisiae, healthy aging'

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Published: 9 March 2023

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1

Su, Wei-Hsuan, Omar Ocegueda, Catherine Choi, Jessica Smith, Kelsey Lee, Yihan Wan, Jacqueline Yao, and Sam Schriner. "SPERMIDINE TOXICITY IN MITOCHONDRIAL DNA-DEFICIENT SACCHAROMYCES CEREVISIAE." Innovation in Aging 6, Supplement_1 (November 1, 2022): 444–45. http://dx.doi.org/10.1093/geroni/igac059.1740.

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Abstract Mitochondrial dysfunction is thought to play a significant role in aging and in manyhuman diseases. Over the last 20 years or so, a number of drugs have been found toextend lifespan in model organisms. Using ethidium bromide to deplete the yeastSaccharomyces cerevisiae of its mitochondrial DNA (mtDNA), we evaluated thedependence on functional mitochondrial in the action of five of these lifespan-extending compounds; dinitrophenol, metformin, rapamycin, resveratrol, andspermidine. None of them extended lifespan in mtDNA-deficient cells, demonstratinga requirement for functional mitochondria in their action. However, we found thatspermidine significantly shortened lifespan in these cells, decreasing the medianlifespan from 6 days to 4 days. Despite this, spermidine, nor any of the othercompounds tested, had any effect of growth rates in mtDNA-deficient cells.Spermidine is thought to extend lifespan through the induction of autophagy. Wepredict that spermidine shortened lifespan in mtDNA-deficient cells through anincreased need for ATP, for which these cells were not able to provide. Given thatmitochondrial dysfunction might be a common feature of aging and disease, ourresults suggest that if spermidine were used as an anti-aging treatment in humans, itmay be harmful.

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2

Wang, Shaoyu. "Leveraging budding yeast Saccharomyces cerevisiae for discovering aging modulation substances for functional food." Functional Foods in Health and Disease 9, no. 5 (May 30, 2019): 297. http://dx.doi.org/10.31989/ffhd.v9i5.575.

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Background: Discovery of bioactive substances contained in functional food and the mechanism of their aging modulation are imperative steps in developing better, potent and safer functional food for promoting health and compression of morbidity in the aging population. Budding yeast (Saccharomyces cerevisiae) is invaluable model organism for aging modulation and bioactive compounds discovery. In this paper we have conceptualised a framework for achieving such aim. This framework consists of four components: discovering targets for aging modulation, discovering and validating caloric restriction mimetics, acting as cellular systems for screening natural products or compounds for aging modulation and being a biological factory for producing bioactive compounds according to the roles the yeast systems play. It have been argued that the component of being a biological factory for producing bioactive compounds has much underexplored which also present an opportunity for new active substance discovery and validation for health promotion in functional food industry.Keywords: Aging modulation, budding yeast, functional food, bioactive substances, cell factory

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Stępień, Karolina, Dominik Wojdyła, Katarzyna Nowak, and Mateusz Mołoń. "Impact of curcumin on replicative and chronological aging in the Saccharomyces cerevisiae yeast." Biogerontology 21, no. 1 (October 28, 2019): 109–23. http://dx.doi.org/10.1007/s10522-019-09846-x.

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Abstract Curcumin is a biologically active compound of vegetable origin which has a hormetic effect. Pro-health and anti-aging properties of curcumin have been known for years. The main benefit of curcumin is thought to be its anti-oxidative action. Despite vast amount of data confirming age-delaying activity of curcumin in various groups of organisms, so far little has been discovered about curcumin’s impact on cell aging in the experimental model of the Saccharomyces cerevisiae budding yeast. We have been able to demonstrate that curcumin significantly increases oxidative stress and accelerates replicative and chronological aging of yeast cells devoid of anti-oxidative protection (with SOD1 and SOD2 gene deletion) and deprived of DNA repair mechanisms (RAD52). Interestingly, curcumin delays aging, probably through hormesis, of the wild-type strain BY4741.

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Ogita, Akira, Wakae Murata, Marina Hasegawa, Ken Yamauchi, Akiko Sakai, Yoshihiro Yamaguchi, Toshio Tanaka, and Ken-ichi Fujita. "PROLONGATION OF HUMAN LIFESPAN BY IMMATURE PEAR EXTRACT MEDIATED SIRTUIN-RELATED GENE EXPRESSION." Innovation in Aging 3, Supplement_1 (November 2019): S97. http://dx.doi.org/10.1093/geroni/igz038.365.

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Abstract Demographics of the world are changing rapidly with older populations growing at an unprecedented rate. Cellular senescence, a decline of cellular function due to aging, causes gradual loss of physiological functions. Several cellular senescence-related chronic diseases, such as metabolic syndrome, cardiovascular disease, cancer, osteoporosis, diabetes, and hypertension, negatively affect the quality of human life. Intervention in the cellular senescence process may reduce these incidences and slow the progression of age-related diseases, while contributing to the longevity of healthy human lifespans. Saccharomyces cerevisiae, the budding yeast, is a simple model system that can provide significant insights into the human genetics and molecular biology of senescence and is considered suitable as a cellular model for research on mammalian cells. The aim of our study was to investigate the anti-aging effects of immature pear fruit extract (IPE) on yeast cells and its possible application to extend healthy lifespan in humans. Anti-aging effects of IPE were investigated using a chronological lifespan assay on S. cerevisiae cells. The chronological lifespan of the yeast treated with IPE at 1% (v/v) was significantly extended than that of untreated cells (p < 0.05). The expression of sirtuin-related genes, which regulate cellular senescence, was examined by reverse transcription-polymerase chain reaction and found to be significantly increased following IPE treatment. These results suggest that sirtuin-related genes have important roles in IPE-regulated lifespan extension, which provides a mechanism by which IPE could affect mammalian cells and potentially extend healthy human lifespans.

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Kitanovic, Ana, and Stefan Wölfl. "Fructose-1,6-bisphosphatase mediates cellular responses to DNA damage and aging in Saccharomyces cerevisiae." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 594, no. 1-2 (February 2006): 135–47. http://dx.doi.org/10.1016/j.mrfmmm.2005.08.005.

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6

Romano, Patrizia, Giacomo Braschi, Gabriella Siesto, Francesca Patrignani, and Rosalba Lanciotti. "Role of Yeasts on the Sensory Component of Wines." Foods 11, no. 13 (June 28, 2022): 1921. http://dx.doi.org/10.3390/foods11131921.

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The aromatic complexity of a wine is mainly influenced by the interaction between grapes and fermentation agents. This interaction is very complex and affected by numerous factors, such as cultivars, degree of grape ripeness, climate, mashing techniques, must chemical–physical characteristics, yeasts used in the fermentation process and their interactions with the grape endogenous microbiota, process parameters (including new non-thermal technologies), malolactic fermentation (when desired), and phenomena occurring during aging. However, the role of yeasts in the formation of aroma compounds has been universally recognized. In fact, yeasts (as starters or naturally occurring microbiota) can contribute both with the formation of compounds deriving from the primary metabolism, with the synthesis of specific metabolites, and with the modification of molecules present in the must. Among secondary metabolites, key roles are recognized for esters, higher alcohols, volatile phenols, sulfur molecules, and carbonyl compounds. Moreover, some specific enzymatic activities of yeasts, linked above all to non-Saccharomyces species, can contribute to increasing the sensory profile of the wine thanks to the release of volatile terpenes or other molecules. Therefore, this review will highlight the main aroma compounds produced by Saccharomyces cerevisiae and other yeasts of oenological interest in relation to process conditions, new non-thermal technologies, and microbial interactions.

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Liu, Gang, Lei Yu, Yordan Martínez, Wenkai Ren, Hengjia Ni, Naif Abdullah Al-Dhabi, Veeramuthu Duraipandiyan, and Yulong Yin. "Dietary Saccharomyces cerevisiae Cell Wall Extract Supplementation Alleviates Oxidative Stress and Modulates Serum Amino Acids Profiles in Weaned Piglets." Oxidative Medicine and Cellular Longevity 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/3967439.

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This research aims to evaluate the effects of dietary supplementation with Saccharomyces cerevisiae cell wall extract (SCCWE) on growth performance, oxidative stress, intestinal morphology, and serum amino acid concentration in weaned piglets. Utilizing a completely randomized design, 40 healthy piglets weaned at 21 d were grouped into 4 experimental treatments with 10 pigs per treatment group. Treatments consisted of a basal diet (T0), a basal diet with a 0.05% SCCWE (T1), a basal diet with a 0.10% SCCWE (T2), and a basal diet with a 0.15% SCCWE (T3). SCCWE supplementation increased the average daily gain and final body weight compared with T0 (P<0.05). SCCWE in T2 and T3 improved the average daily feed intake and decreased the feed/gain ratio compared with T1 and T2 (P<0.05). SCCWE decreased serum malondialdehyde (MDA) and increased activities of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD) significantly compared to T0 (P<0.05). SCCWE increased the concentration of Ile compared to T0 (P<0.05). Moreover, the concentrations of Leu, Phe, and Arg were higher in T2 and T3 (P<0.05). These findings indicate beneficial effects of SCCWE supplementation on growth performance, the concentration of some essential amino acids, and alleviation of oxidative stress in weaned piglets.

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Silva, Rayssa H. da, Renata F. Barabasz, Monica C. Sustakowski, Odair J. Kuhn, Jeferson C. Carvalho, Willian dos Reis, José R. Stangarlin, and Vinícius H. D. de Oliveira. "Microbiolization of Seeds and Aerial Application With Yeasts for Disease Control in Wheat." Journal of Agricultural Science 12, no. 10 (September 15, 2020): 307. http://dx.doi.org/10.5539/jas.v12n10p307.

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Wheat productivity is compromised by the number of diseases that affect it. The diseases control is basically effected by the use of fungicides, however, biological control has become important due especially to the demand for foods free of chemical compounds. The objective of this work was to evaluate the efficiency of yeasts in reducing diseases through the treatment of wheat seeds and the spraying of plants in the field. The tests were carried out in the field and laboratory, with the yeasts Candida albicans, Cryptococcus laurentii, Pichia guilliermondii, Rhodotorula glutinis, Zygoascus hellenicus and Saccharomyces cerevisiae compared with fungicide (carbendazim) and control with water. In the laboratory, seed health, germination, germination speed index, cold test and accelerated aging were analyzed. In the field, seed treatment and aerial application were carried out when the flag leaf was emitted and the occurrence of diseases, chlorophyll content, production components, productivity and production quality was evaluated. For the variables of production and quality of the wheat in field, the yeast Z. hellenicus was efficient for productivity resembling the fungicide. For the severity of diseases in field, parameters related to germination and seed health, yeasts were not efficient.

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Harris, Rachel E., and Troy A. A. Harkness. "Abstract B024: Increasing cellular longevity in budding yeast by activating the Anaphase Promoting Complex." Cancer Research 83, no. 2_Supplement_1 (January 15, 2023): B024. http://dx.doi.org/10.1158/1538-7445.agca22-b024.

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Abstract The Anaphase Promoting Complex (APC), a large and highly conserved ubiquitin ligase, is involved in genome stability, resistance to cancer progression, and has an important role in promoting health and lifespan. While best known for its function during the events of the cell cycle, the APC also has important roles outside of mitosis in aging cells. Understanding changes in function of the APC, and how these changes can be optimized, may increase our knowledge of cellular longevity and how it can be enhanced. We hypothesize that the APC loses function with age, and that the introduction of biological activators may aid to rescue these effects, improving the overall health of the cell to extend longevity. The abundance of orthologous genes and pathways between eukaryotes, complemented by the relative ease of genetic manipulation, allows for rapid advancement of research by deciphering gene functions in a yeast model to predict human disease and aging. By extension, increasing cell health in multicellular organisms will ultimately enhance the health of the entire organism. A dual screen was performed in the Harkness Lab on a library of short, random peptides to identify those which reproducibly interact with APC subunits, with particular interest on those which have an affect on function. Current research has found that several of these peptides are effective at extending cellular lifespan and suppressing APC mutant phenotypes, providing a novel means of exploring APC function in aging cells. Utilizing this direct biological activation of the APC, in addition to commercially available activators that function indirectly, I aim to identify potential therapeutic agents that can increase APC activity and extend lifespan utilizing Saccharomyces cerevisiae as a model organism. Citation Format: Rachel E. Harris, Troy A. A. Harkness. Increasing cellular longevity in budding yeast by activating the Anaphase Promoting Complex [abstract]. In: Proceedings of the AACR Special Conference: Aging and Cancer; 2022 Nov 17-20; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2022;83(2 Suppl_1):Abstract nr B024.

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Al Bataineh, Mohammad Tahseen, Ayman Alzaatreh, Rima Hajjo, Bayan Hassan Banimfreg, and Nihar Ranjan Dash. "Compositional changes in human gut microbiota reveal a putative role of intestinal mycobiota in metabolic and biological decline during aging." Nutrition and Healthy Aging 6, no. 4 (April 13, 2022): 269–83. http://dx.doi.org/10.3233/nha-210130.

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BACKGROUND: Age-related alterations in the composition and function of gut microbiota may influence human health and disease mechanisms. However, connections between compositional changes in gut bacterial and fungal communities, and their role in the aging process, remain poorly understood. OBJECTIVE: Compare the gut microbiota and mycobiota composition in different age groups and evaluate the functionality. METHODS: In this study, we performed 16S rRNA and ITS2 gene-based microbial profiling analysis and shotgun metagenomics using the NextSeq platform. RESULTS: We observed a shift in compositional changes of human gut microbiota with age. Older individuals revealed a significantly different gut microbiota profile compared to younger individuals. For example, gut microbiota composition of the older individuals showed increase in genera Bacteroides, Blautia, Ruminococcaceae, and Escherichia coli. Additionally, older individuals had significant reduction in fungi belonging to saccharomyces cerevisiae and candida albicans in comparison to their younger counterparts. Moreover, metagenomics functional profiling analysis using shotgun metagenomics sequencing data showed substantial differences in the enrichment of 48 pathways between the young and older age groups. Metabolic pathways such as amino acid biosynthesis, carbohydrate metabolism, cell structure biosynthesis and vitamin biosynthesis were declined in the older age group, in comparison with the younger individuals. CONCLUSIONS: The identified differences provide a new insight to enrich our understanding of age-related changes in gut microbiota, their metabolic capabilities, and potential impact on health and disease conditions.

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Namkoong, Jin, Dale Kern, and Helen Knaggs. "Assessment of Human Skin Gene Expression by Different Blends of Plant Extracts with Implications to Periorbital Skin Aging." International Journal of Molecular Sciences 19, no. 11 (October 26, 2018): 3349. http://dx.doi.org/10.3390/ijms19113349.

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Since the skin is the major protective barrier of the body, it is affected by intrinsic and extrinsic factors. Environmental influences such as ultraviolet (UV) irradiation, pollution or dry/cold air are involved in the generation of radical oxygen species (ROS) and impact skin aging and dermal health. Assessment of human skin gene expression and other biomarkers including epigenetic factors are used to evaluate the biological/molecular activities of key compounds in cosmetic formulas. The objective of this study was to quantify human gene expression when epidermal full-thickness skin equivalents were exposed to: (a) a mixture of betaine, pentylene glycol, Saccharomyces cerevisiae and Rhodiola rosea root extract (BlendE) for antioxidant, skin barrier function and oxidative stress (with hydrogen peroxide challenge); and (b) a mixture of Narcissus tazetta bulb extract and Schisandra chinensis fruit extract (BlendIP) for various biomarkers and microRNA analysis. For BlendE, several antioxidants, protective oxidative stress biomarkers and many skin barrier function parameters were significantly increased. When BlendE was evaluated, the negative impact of the hydrogen peroxide was significantly reduced for the matrix metalloproteinases (MMP 3 and MMP 12), the skin aging and oxidative stress biomarkers, namely FBN2, ANXA1 and HGF. When BlendIP was tested for cell proliferation and dermal structural components to enhance the integrity of the skin around the eyes: 8 growth factors, 7 signaling, 7 structural/barrier function and 7 oxidative stress biomarkers were significantly increased. Finally, when BlendIP was tested via real-time RT-PCR for microRNA expression: miR-146a, miR-22, miR155, miR16 and miR21 were all significantly increased over control levels. Therefore, human skin gene expression studies are important tools to assess active ingredient compounds such as plant extract blends to advance dermal hypotheses toward validating cosmetic formulations with botanical molecules.

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Mołoń, Mateusz, Karolina Stępień, Patrycja Kielar, Bela Vasileva, Bonka Lozanska, Dessislava Staneva, Penyo Ivanov, et al. "Actin-Related Protein 4 and Linker Histone Sustain Yeast Replicative Ageing." Cells 11, no. 17 (September 3, 2022): 2754. http://dx.doi.org/10.3390/cells11172754.

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Ageing is accompanied by dramatic changes in chromatin structure organization and genome function. Two essential components of chromatin, the linker histone Hho1p and actin-related protein 4 (Arp4p), have been shown to physically interact in Saccharomyces cerevisiae cells, thus maintaining chromatin dynamics and function, as well as genome stability and cellular morphology. Disrupting this interaction has been proven to influence the stability of the yeast genome and the way cells respond to stress during chronological ageing. It has also been proven that the abrogated interaction between these two chromatin proteins elicited premature ageing phenotypes. Alterations in chromatin compaction have also been associated with replicative ageing, though the main players are not well recognized. Based on this knowledge, here, we examine how the interaction between Hho1p and Arp4p impacts the ageing of mitotically active yeast cells. For this purpose, two sets of strains were used—haploids (WT(n), arp4, hho1Δ and arp4 hho1Δ) and their heterozygous diploid counterparts (WT(2n), ARP4/arp4, HHO1/hho1Δ and ARP4 HHO1/arp4 hho1Δ)—for the performance of extensive morphological and physiological analyses during replicative ageing. These analyses included a comparative examination of the yeast cells’ chromatin structure, proliferative and reproductive potential, and resilience to stress, as well as polysome profiles and chemical composition. The results demonstrated that the haploid chromatin mutants arp4 and arp4 hho1Δ demonstrated a significant reduction in replicative and total lifespan. These findings lead to the conclusion that the importance of a healthy interaction between Arp4p and Hho1p in replicative ageing is significant. This is proof of the concomitant importance of Hho1p and Arp4p in chronological and replicative ageing.

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Lewis, Kim. "Programmed Death in Bacteria." Microbiology and Molecular Biology Reviews 64, no. 3 (September 1, 2000): 503–14. http://dx.doi.org/10.1128/mmbr.64.3.503-514.2000.

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SUMMARY Programmed cell death (PCD) in bacteria plays an important role in developmental processes, such as lysis of the mother cell during sporulation of Bacillus subtilis and lysis of vegetative cells in fruiting body formation of Myxococcus xanthus. The signal transduction pathway leading to autolysis of the mother cell includes the terminal sporulation sigma factor EςK, which induces the synthesis of autolysins CwlC and CwlH. An activator of autolysin in this and other PCD processes is yet to be identified. Autolysis plays a role in genetic exchange in Streptococcus pneumoniae, and the gene for the major autolysin, lytA, is located in the same operon with recA. DNA from lysed cells is picked up by their neighbors and recombined into the chromosome by RecA. LytA requires an unknown activator controlled by a sensory kinase, VncS. Deletion of vncS inhibits autolysis and also decreases killing by unrelated antibiotics. This observation suggests that PCD in bacteria serves to eliminate damaged cells, similar to apoptosis of defective cells in metazoa. The presence of genes affecting survival without changing growth sensitivity to antibiotics (vncS, lytA, hipAB, sulA, and mar) indicates that bacteria are able to control their fate. Elimination of defective cells could limit the spread of a viral infection and donate nutrients to healthy kin cells. An altruistic suicide would be challenged by the appearance of asocial mutants without PCD and by the possibility of maladaptive total suicide in response to a uniformly present lethal factor or nutrient depletion. It is proposed that a low rate of mutation serves to decrease the probability that asocial mutants without PCD will take over the population. It is suggested that PCD is disabled in persistors, rare cells that are resistant to killing, to ensure population survival. It is suggested that lack of nutrients leads to the stringent response that suppresses PCD, producing a state of tolerance to antibiotics, allowing cells to discriminate between nutrient deprivation and unrepairable damage. High levels of persistors are apparently responsible for the extraordinary survival properties of bacterial biofilms, and genes affecting persistence appear to be promising targets for development of drugs aimed at eradicating recalcitrant infections. PCD in unicellular eukaryotes is also considered, including aging in Saccharomyces cerevisiae. Apoptosis-like elimination of defective cells in S. cerevisiae and protozoa suggests that all unicellular life forms evolved altruistic programmed death that serves a variety of useful functions.

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Holbrook, M. A., and J. R. Menninger. "Erythromycin Slows Aging of Saccharomyces cerevisiae." Journals of Gerontology Series A: Biological Sciences and Medical Sciences 57, no. 1 (January 1, 2002): B29—B36. http://dx.doi.org/10.1093/gerona/57.1.b29.

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15

Kennedy, Brian K., and Leonard Guarente. "Genetic analysis of aging in Saccharomyces cerevisiae." Trends in Genetics 12, no. 9 (September 1996): 355–59. http://dx.doi.org/10.1016/s0168-9525(96)80018-7.

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16

Cohen, Aviv, Esther Weindling, Efrat Rabinovich, Iftach Nachman, Shai Fuchs, Silvia Chuartzman, Lihi Gal, Maya Schuldiner, and Shoshana Bar-Nun. "Water-Transfer Slows Aging in Saccharomyces cerevisiae." PLOS ONE 11, no. 2 (February 10, 2016): e0148650. http://dx.doi.org/10.1371/journal.pone.0148650.

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Longo, Valter D., Gerald S. Shadel, Matt Kaeberlein, and Brian Kennedy. "Replicative and Chronological Aging in Saccharomyces cerevisiae." Cell Metabolism 16, no. 1 (July 2012): 18–31. http://dx.doi.org/10.1016/j.cmet.2012.06.002.

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Peters, Theodore W., Matthew J. Rardin, Gregg Czerwieniec, Uday S. Evani, Pedro Reis-Rodrigues, Gordon J. Lithgow, Sean D. Mooney, Bradford W. Gibson, and Robert E. Hughes. "Tor1 regulates protein solubility in Saccharomyces cerevisiae." Molecular Biology of the Cell 23, no. 24 (December 15, 2012): 4679–88. http://dx.doi.org/10.1091/mbc.e12-08-0620.

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Accumulation of insoluble protein in cells is associated with aging and aging-related diseases; however, the roles of insoluble protein in these processes are uncertain. The nature and impact of changes to protein solubility during normal aging are less well understood. Using quantitative mass spectrometry, we identify 480 proteins that become insoluble during postmitotic aging in Saccharomyces cerevisiae and show that this ensemble of insoluble proteins is similar to those that accumulate in aging nematodes. SDS-insoluble protein is present exclusively in a nonquiescent subpopulation of postmitotic cells, indicating an asymmetrical distribution of this protein. In addition, we show that nitrogen starvation of young cells is sufficient to cause accumulation of a similar group of insoluble proteins. Although many of the insoluble proteins identified are known to be autophagic substrates, induction of macroautophagy is not required for insoluble protein formation. However, genetic or chemical inhibition of the Tor1 kinase is sufficient to promote accumulation of insoluble protein. We conclude that target of rapamycin complex 1 regulates accumulation of insoluble proteins via mechanisms acting upstream of macroautophagy. Our data indicate that the accumulation of proteins in an SDS-insoluble state in postmitotic cells represents a novel autophagic cargo preparation process that is regulated by the Tor1 kinase.

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D'Mello, N. P., and S. M. Jazwinski. "Telomere length constancy during aging of Saccharomyces cerevisiae." Journal of Bacteriology 173, no. 21 (1991): 6709–13. http://dx.doi.org/10.1128/jb.173.21.6709-6713.1991.

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Chen, Xiao-Fen, Fei-Long Meng, and Jin-Qiu Zhou. "Telomere Recombination Accelerates Cellular Aging in Saccharomyces cerevisiae." PLoS Genetics 5, no. 6 (June 26, 2009): e1000535. http://dx.doi.org/10.1371/journal.pgen.1000535.

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Blomme, Arnaud, Allan Mac'Cord, Francis E. Sluse, and Gregory Mathy. "Proteomic evolution of Saccharomyces cerevisiae during chronological aging." Biochimica et Biophysica Acta (BBA) - Bioenergetics 1797 (July 2010): 58. http://dx.doi.org/10.1016/j.bbabio.2010.04.189.

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Jazwinski, S. M. "Aging and senescence of the budding yeast Saccharomyces cerevisiae." Molecular Microbiology 4, no. 3 (March 1990): 337–43. http://dx.doi.org/10.1111/j.1365-2958.1990.tb00601.x.

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Setiyoningrum, F., G. Priadi, and F. Afiati. "Chemical properties of solo black garlic fermented by Saccharomyces cerevisiae." IOP Conference Series: Earth and Environmental Science 976, no. 1 (February 1, 2022): 012044. http://dx.doi.org/10.1088/1755-1315/976/1/012044.

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Abstract Several research report shown a fermentation could increase or produce a new compound in a material. Research of solo black garlic involved fermentation process of fresh garlic prior to aging process was limited. The aim of this research was to examine chemical properties of solo black garlic fermented in medium containing S. cerevisiae before its aged. The variance result shown that there was an interaction between fermentation and aging time on its antioxidant capacity, total flavonoids and total polyphenol significantly. The treatment of fermentation of fresh solo garlic in medium containing S. cerevisiae for 4 days and continued by aging for 21 days gave the best chemical properties on solo black garlic, with 95.88% of antioxidant capacity, 89.74 mg GAE/g of total flavonoid and 108.92 mg QE/g of total polyphenol. Comparing with control-without fermented by S. cerevisiae, those treatment gave a better chemical properties. Futhermore, profiling by LC-MS-QTOF revealed that several alkaloids, polyphenol and flavonoids compound was founded in those sample. Thatfindings indicate that fermented by S. cerevisiae prior to aging process could be considered for increasing of solo black garlic’s functional properties.

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McCleary, David F., and Jasper Rine. "Nutritional Control of Chronological Aging and Heterochromatin in Saccharomyces cerevisiae." Genetics 205, no. 3 (January 6, 2017): 1179–93. http://dx.doi.org/10.1534/genetics.116.196485.

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Sorokin, Maksim, Dmitry Knorre, and Fedor Severin. "Early manifestations of replicative aging in the yeast Saccharomyces cerevisiae." Microbial Cell 1, no. 1 (January 6, 2014): 37–42. http://dx.doi.org/10.15698/mic2014.01.122.

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Yiu, G., A. McCord, A. Wise, R. Jindal, J. Hardee, A. Kuo, M. Y. Shimogawa, et al. "Pathways Change in Expression During Replicative Aging in Saccharomyces cerevisiae." Journals of Gerontology Series A: Biological Sciences and Medical Sciences 63, no. 1 (January 1, 2008): 21–34. http://dx.doi.org/10.1093/gerona/63.1.21.

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Ashrafi, K., D. Sinclair, J. I. Gordon, and L. Guarente. "Passage through stationary phase advances replicative aging in Saccharomyces cerevisiae." Proceedings of the National Academy of Sciences 96, no. 16 (August 3, 1999): 9100–9105. http://dx.doi.org/10.1073/pnas.96.16.9100.

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MacCord, Allan, Gregory Mathy, Pierre Leprince, Edwin de Pauw, and Francis E. Sluse. "S14.7 Impact of chronological aging on mitoproteome of Saccharomyces cerevisiae." Biochimica et Biophysica Acta (BBA) - Bioenergetics 1777 (July 2008): S101. http://dx.doi.org/10.1016/j.bbabio.2008.05.395.

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Bitterman, Kevin J., Oliver Medvedik, and David A. Sinclair. "Longevity Regulation in Saccharomyces cerevisiae: Linking Metabolism, Genome Stability, and Heterochromatin." Microbiology and Molecular Biology Reviews 67, no. 3 (September 2003): 376–99. http://dx.doi.org/10.1128/mmbr.67.3.376-399.2003.

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SUMMARY When it was first proposed that the budding yeast Saccharomyces cerevisiae might serve as a model for human aging in 1959, the suggestion was met with considerable skepticism. Although yeast had proved a valuable model for understanding basic cellular processes in humans, it was difficult to accept that such a simple unicellular organism could provide information about human aging, one of the most complex of biological phenomena. While it is true that causes of aging are likely to be multifarious, there is a growing realization that all eukaryotes possess surprisingly conserved longevity pathways that govern the pace of aging. This realization has come, in part, from studies of S. cerevisiae, which has emerged as a highly informative and respected model for the study of life span regulation. Genomic instability has been identified as a major cause of aging, and over a dozen longevity genes have now been identified that suppress it. Here we present the key discoveries in the yeast-aging field, regarding both the replicative and chronological measures of life span in this organism. We discuss the implications of these findings not only for mammalian longevity but also for other key aspects of cell biology, including cell survival, the relationship between chromatin structure and genome stability, and the effect of internal and external environments on cellular defense pathways. We focus on the regulation of replicative life span, since recent findings have shed considerable light on the mechanisms controlling this process. We also present the specific methods used to study aging and longevity regulation in S. cerevisiae.

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Kirchman, Paul A., Sangkyu Kim, Chi-Yung Lai, and S. Michal Jazwinski. "Interorganelle Signaling Is a Determinant of Longevity in Saccharomyces cerevisiae." Genetics 152, no. 1 (May 1, 1999): 179–90. http://dx.doi.org/10.1093/genetics/152.1.179.

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Abstract Replicative capacity, which is the number of times an individual cell divides, is the measure of longevity in the yeast Saccharomyces cerevisiae. In this study, a process that involves signaling from the mitochondrion to the nucleus, called retrograde regulation, is shown to determine yeast longevity, and its induction resulted in postponed senescence. Activation of retrograde regulation, by genetic and environmental means, correlated with increased replicative capacity in four different S. cerevisiae strains. Deletion of a gene required for the retrograde response, RTG2, eliminated the increased replicative capacity. RAS2, a gene previously shown to influence longevity in yeast, interacts with retrograde regulation in setting yeast longevity. The molecular mechanism of aging elucidated here parallels the results of genetic studies of aging in nematodes and fruit flies, as well as the caloric restriction paradigm in mammals, and it underscores the importance of metabolic regulation in aging, suggesting a general applicability.

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Velenosi, Matteo, Pasquale Crupi, Rocco Perniola, Antonio Domenico Marsico, Antonella Salerno, Hervè Alexandre, Nicoletta Archidiacono, Mario Ventura, and Maria Francesca Cardone. "Color Stabilization of Apulian Red Wines through the Sequential Inoculation of Starmerella bacillaris and Saccharomyces cerevisiae." Molecules 26, no. 4 (February 9, 2021): 907. http://dx.doi.org/10.3390/molecules26040907.

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Mixed fermentation using Starmerella bacillaris and Saccharomyces cerevisiae has gained attention in recent years due to their ability to modulate the qualitative parameters of enological interest, such as the color intensity and stability of wine. In this study, three of the most important red Apulian varieties were fermented through two pure inoculations of Saccharomyces cerevisiae strains or the sequential inoculation of Saccharomyces cerevisiae after 48 h from Starmerella bacillaris. The evolution of anthocyanin profiles and chromatic characteristics were determined in the produced wines at draining off and after 18 months of bottle aging in order to assess the impact of the different fermentation protocols on the potential color stabilization and shelf-life. The chemical composition analysis showed titratable acidity and ethanol content exhibiting marked differences among wines after fermentation and aging. The 48 h inoculation delay produced wines with higher values of color intensity and color stability. This was ascribed to the increased presence of compounds, such as stable A-type vitisins and reddish/violet ethylidene-bridge flavonol-anthocyanin adducts, in the mixed fermentation. Our results proved that the sequential fermentation of Starmerella bacillaris and Saccharomyces cerevisiae could enhance the chromatic profile as well as the stability of the red wines, thus improving their organoleptic quality.

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Benetti, Fábia, Thanise Antunes Dias, Jorge Alberto Vieira Costa, and Telma Elita Bertolin. "Caloric restriction and Spirulina platensis extract against ferrous ion (Fe2+) in the aging of Saccharomyces cerevisiae cells deleted to the SIR2 gene." Research, Society and Development 9, no. 8 (July 24, 2020): e662986210. http://dx.doi.org/10.33448/rsd-v9i8.6210.

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The aging process is aggravated by the presence of a high load of oxidative stress associated with the body's imbalance concerning certain metals, with emphasis on iron. Spirulina platensis extract (SP) and caloric restriction (CR) are nutritional interventions capable to mitigate the effects of aging-related diseases. The objective of this study was to determine the effects of SP and CR against ferrous ion on the aging of Saccharomyces cerevisiae deleted of SIR2 gene. Methods: Saccharomyces cerevisiae standard (WT) and sir2Δ strains, cultured in 2% or 0.5% (CR) glucose YPD media, whether exposed to 0.8 mg/mL SP and 1mM Fe2+. Cell viability and lipoperoxidation were analyzed. Results showed reduced cell survival and increased lipid peroxidation in the SIR2 gene deletion. Statistically significant results were found after aging for WT, SP, CR, SP + Fe2+, CR + Fe2+ treatments. The therapies CR and SP showed a protective effect against ferrous ion.

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Arlia-Ciommo, Anthony, Anna Leonov, Amanda Piano, Veronika Svistkova, and Vladimir Titorenko. "Cell-autonomous mechanisms of chronological aging in the yeast Saccharomyces cerevisiae." Microbial Cell 1, no. 6 (June 2, 2014): 163–78. http://dx.doi.org/10.15698/mic2014.06.152.

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Lefevre, Sophie D., Carlo W. Roermund, Ronald J. A. Wanders, Marten Veenhuis, and Ida J. Klei. "The significance of peroxisome function in chronological aging of Saccharomyces cerevisiae." Aging Cell 12, no. 5 (July 8, 2013): 784–93. http://dx.doi.org/10.1111/acel.12113.

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Kaya, Alaattin, Alexei V. Lobanov, and Vadim N. Gladyshev. "Evidence that mutation accumulation does not cause aging in Saccharomyces cerevisiae." Aging Cell 14, no. 3 (February 22, 2015): 366–71. http://dx.doi.org/10.1111/acel.12290.

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van der Laan, Kiran J., Julie Naulleau, Viraj G. Damle, Alina Sigaeva, Nicolas Jamot, Felipe P. Perona-Martinez, Mayeul Chipaux, and Romana Schirhagl. "Toward Using Fluorescent Nanodiamonds To Study Chronological Aging in Saccharomyces cerevisiae." Analytical Chemistry 90, no. 22 (October 22, 2018): 13506–13. http://dx.doi.org/10.1021/acs.analchem.8b03431.

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Fabrizio, Paola, Luisa Battistella, Raffaello Vardavas, Cristina Gattazzo, Lee-Loung Liou, Alberto Diaspro, Janis W. Dossen, Edith Butler Gralla, and Valter D. Longo. "Superoxide is a mediator of an altruistic aging program in Saccharomyces cerevisiae." Journal of Cell Biology 166, no. 7 (September 27, 2004): 1055–67. http://dx.doi.org/10.1083/jcb.200404002.

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Aging is believed to be a nonadaptive process that escapes the force of natural selection. Here, we challenge this dogma by showing that yeast laboratory strains and strains isolated from grapes undergo an age- and pH-dependent death with features of mammalian programmed cell death (apoptosis). After 90–99% of the population dies, a small mutant subpopulation uses the nutrients released by dead cells to grow. This adaptive regrowth is inversely correlated with protection against superoxide toxicity and life span and is associated with elevated age-dependent release of nutrients and increased mutation frequency. Computational simulations confirm that premature aging together with a relatively high mutation frequency can result in a major advantage in adaptation to changing environments. These results suggest that under conditions that model natural environments, yeast organisms undergo an altruistic and premature aging and death program, mediated in part by superoxide. The role of similar pathways in the regulation of longevity in organisms ranging from yeast to mice raises the possibility that mammals may also undergo programmed aging.

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Ha, Cheol Woong, and Won-Ki Huh. "The implication of Sir2 in replicative aging and senescence in Saccharomyces cerevisiae." Aging 3, no. 3 (March 13, 2011): 319–24. http://dx.doi.org/10.18632/aging.100299.

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BALOGU, TOCHUKWU VINCENT`. "YEAST DYNAMICS AND PHYSIOCHEMICAL EVALUATION OF CARROT WINE PRODUCED WITH Saccharomyces cerevisiae." Fungal Territory 3, no. 3 (August 13, 2020): 27–29. http://dx.doi.org/10.36547/ft.2020.3.3.27-29.

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Yeast dynamics and physiochemical evaluation of carrot wine produced with Saccharomyces cerevisiae were assessed. Fresh ripe and health carrot (6kg) were sequentially processed (washed, preheated, blended and sieved) into juice and fermented for 60 days with Saccharomyces cerevisiae. Airtight glass jars composed of juice (2000g), distilled water (2000mL) and sugar (200g) at controlled temperature (20 -25 0C) was used for fermentation. Wines were clarified (siphoning), aged (45 days) and pasteurized (500C – 600C) to stop fermentation. Proximate analysis, yeast dynamics, physiochemical and wine qualities were assessed. Result showed that juice extraction process reconstitute nutritional composition of carrot, such that moisture, ash and total carbohydrates increased, while others (fat, crude fiber and crude protein) decreased. A trendy progressive yeast dynamic model of Yeast load = -0.195 (Day) 2 + 1.822 (Day) + 4.566 with coefficient (R² = 0.907) was observed. Fermentation significantly decreased pH and increased total acidity. Observed wine qualities include alcoholic content (7.88 - 9.19%v/v), attenuation (121% - 142%) and calories (0%). Clarification and ageing have diminishing effect on alcohol content. Carrot wine was judged as physically appealing moderate alcoholic beverage, with smooth consistent taste (authors' opinion), and could be modeled with yeast dynamics. Thus this wine is recommended to calories sensitive people.

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Biliński, Tomasz, and Grzegorz Bartosz. "Hypothesis: cell volume limits cell divisions." Acta Biochimica Polonica 53, no. 4 (November 14, 2006): 833–35. http://dx.doi.org/10.18388/abp.2006_3313.

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Mammalian somatic cells and also cells of the yeast Saccharomyces cerevisiae are capable of undergoing a limited number of divisions. Reaching the division limit is referred to, apparently not very fortunately, as replicative aging. A common feature of S. cerevisiae cells and fibroblasts approaching the limit of cell divisions in vitro is attaining giant volumes. In yeast cells this phenomenon is an inevitable consequence of budding so it is not causally related to aging. Therefore, reaching a critically large cell volume may underlie the limit of cell divisions. A similar phenomenon may limit the number of cell divisions of cultured mammalian cells. The term replicative (generative) aging may be therefore illegitimate.

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Yang, Emily J., and Liza A. Pon. "Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiae." STAR Protocols 3, no. 3 (September 2022): 101599. http://dx.doi.org/10.1016/j.xpro.2022.101599.

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Samokhvalov, Victor, Vladimir Ignatov, and Marie Kondrashova. "Reserve carbohydrates maintain the viability of Saccharomyces cerevisiae cells during chronological aging." Mechanisms of Ageing and Development 125, no. 3 (March 2004): 229–35. http://dx.doi.org/10.1016/j.mad.2003.12.006.

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Jakubowski, Witold, Tomasz Biliński, and Grzegorz Bartosz. "Oxidative stress during aging of stationary cultures of the yeast Saccharomyces cerevisiae." Free Radical Biology and Medicine 28, no. 5 (March 2000): 659–64. http://dx.doi.org/10.1016/s0891-5849(99)00266-x.

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O'Laughlin, Richard, Meng Jin, Yang Li, Lorraine Pillus, Lev S. Tsimring, Jeff Hasty, and Nan Hao. "Advances in quantitative biology methods for studying replicative aging in Saccharomyces cerevisiae." Translational Medicine of Aging 4 (2020): 151–60. http://dx.doi.org/10.1016/j.tma.2019.09.002.

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Tahara, Erich B., Fernanda M. Cunha, Thiago O. Basso, Bianca E. Della Bianca, Andreas K. Gombert, and Alicia J. Kowaltowski. "Calorie Restriction Hysteretically Primes Aging Saccharomyces cerevisiae toward More Effective Oxidative Metabolism." PLoS ONE 8, no. 2 (February 11, 2013): e56388. http://dx.doi.org/10.1371/journal.pone.0056388.

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Molon, Mateusz, and Renata Zadrag-Tecza. "Effect of temperature on replicative aging of the budding yeast Saccharomyces cerevisiae." Biogerontology 17, no. 2 (October 20, 2015): 347–57. http://dx.doi.org/10.1007/s10522-015-9619-3.

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Grzelak, Agnieszka, Ewa Macierzyńska, and Grzegorz Bartosz. "Accumulation of oxidative damage during replicative aging of the yeast Saccharomyces cerevisiae." Experimental Gerontology 41, no. 9 (September 2006): 813–18. http://dx.doi.org/10.1016/j.exger.2006.06.049.

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Motizuki, Mitsuyoshi, and Kunio Tsurugi. "The effect of aging on protein synthesis in the yeast Saccharomyces cerevisiae." Mechanisms of Ageing and Development 64, no. 3 (July 1992): 235–45. http://dx.doi.org/10.1016/0047-6374(92)90081-n.

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Khandaker, AM, and A. Koc. "Deletion of mitochondrial inorganic pyrophosphatase gene extends life span in haploid yeast (Saccharomyces cerevisiae)." Journal of Biodiversity Conservation and Bioresource Management 3, no. 2 (April 25, 2018): 69–76. http://dx.doi.org/10.3329/jbcbm.v3i2.36030.

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Aging is a universal but poorly understood biological process and its underlying mechanisms are under intensive study. Mitochondria have a central role in the studies of aging hence they supply the majority of the organisms’ energy requirement from biological fuels. However, the role of mitochondria in the aging process is more complicated than the proposed theories of aging. We addressed a question by asking whether deletion to mitochondrial metabolism genes can extend life span in haploid cell of Saccharomyces cerevisiae (yeast). In this study, strains derived from the yeast open reading frame (ORF) deletions were screened through replicative aging assay in order to identify the mitochondrial metabolism genes that increase life span. This has resulted in the isolation of a long living (22% extended life span) mutant, ppa2Δ, which lack mitochondrial inorganic pyro phosphatase gene. The mitochondrial morphology of this long living mutant was analyzed by fluorescence microscopy. Compared to serpentine nature of wild type mitochondria, a different dynamics and distribution pattern was viewed, i.e. mitochondria were aggregated and formed colonies within the cytoplasm of this long lived cell. The aggregated mitochondrial mass was found to be intact from the young to old stage of life. Thus, this investigation reveals the longevity role of the mutant form of the gene PPA2 through an alteration of mitochondrial morphology.J. Biodivers. Conserv. Bioresour. Manag. 2017, 3(2): 69-76

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Bhattacharya, Somanon, Tejas Bouklas, and Bettina C. Fries. "Replicative Aging in Pathogenic Fungi." Journal of Fungi 7, no. 1 (December 25, 2020): 6. http://dx.doi.org/10.3390/jof7010006.

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Candida albicans, Candida auris, Candida glabrata, and Cryptococcus neoformans are pathogenic yeasts which can cause systemic infections in immune-compromised as well as immune-competent individuals. These yeasts undergo replicative aging analogous to a process first described in the nonpathogenic yeast Saccharomyces cerevisiae. The hallmark of replicative aging is the asymmetric cell division of mother yeast cells that leads to the production of a phenotypically distinct daughter cell. Several techniques to study aging that have been pioneered in S. cerevisiae have been adapted to study aging in other pathogenic yeasts. The studies indicate that aging is relevant for virulence in pathogenic fungi. As the mother yeast cell progressively ages, every ensuing asymmetric cell division leads to striking phenotypic changes, which results in increased antifungal and antiphagocytic resistance. This review summarizes the various techniques that are used to study replicative aging in pathogenic fungi along with their limitations. Additionally, the review summarizes some key phenotypic variations that have been identified and are associated with changes in virulence or resistance and thus promote persistence of older cells.

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