Relevant bibliographies by topics / Saccharomyces cerevisiae

Academic literature on the topic 'Saccharomyces cerevisiae'

Author: Grafiati
Published: 4 June 2021
Last updated: 7 July 2024

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Journal articles on the topic "Saccharomyces cerevisiae"

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Marinov, Luka, Ana Jeromel, Ivana Tomaz, Darko Preiner, and Ana Marija Jagatić Korenika. "Učinak sekvencijalne fermentacije s kvascima Lachancea thermotelerans i Torulaspora delbrueckii na kemijski sastav vina ´Malvazija istarska´." Glasnik zaštite bilja 44, no. 4 (July 12, 2021): 56–66. http://dx.doi.org/10.31727/gzb.44.4.8.

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Suočavajući se sa sve drastičnijim utjecajem klimatskih čimbenika na kemijski sastav grožđa, enologija traži i proučava nove metode u tehnologiji proizvodnje vina, posebice bijelih, kako bi se očuvale primarne arome te postigla ravnoteža između alkoholne jakosti i ukupne kiselosti. Kao jedno od rješenja nudi se primjena ne- Saccharomyces kvasaca. U ovom istraživanju analiziran je utjecaj sekvencijalne inokulacije komercijalnih sojeva Torulospora delbrueckii i Lachancea thermotolerans sa sojem kvasca Saccharomycem cerevisiae na vino ´Malvazija istarska´. Istraživanje je obuhvatilo inokulacije mošta s ne-Saccharomyces kvascima, a 48 h kasnije i sa sojem S. cerevisae te kontrolnu varijantu isključivo sa S. cerevisae. Ne-Saccharomyces kvasci utjecali su značajno na koncentraciju alkohola, mliječne kiseline te pH vrijednost. Fermentacija sa S. cerevisiae utjecala je na višu koncentraciju ukupnih aromatskih spojeva u vinu. Intenziteti boje i mirisa najbolje su ocijenjeni u kontrolnom uzorku, a metodom redoslijeda najbolje je rangirana ´Malvazija´ iz tretmana T. delbrueckii/ S. cerevisiae.
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Vaštík, Peter, Daniela Šmogrovičová, Valentína Kafková, Pavol Sulo, Katarína Furdíková, and Ivan Špánik. "Production and characterisation of non-alcoholic beer using special yeast." KVASNY PRUMYSL 66, no. 5 (October 15, 2020): 336–44. http://dx.doi.org/10.18832/kp2019.66.336.

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Non-Saccharomyces yeast strains Saccharomycodes ludwigii, Schizosaccharomyces pombe, Lachancea fermentati and Pichia angusta together with a hybrid yeast strain cross-bred between genetically modified Saccharomyces cerevisiae W303-1A G418R and Saccharomyces eubayanus as well as the parent yeasts of the hybrid were studied for potential use for non-alcoholic beer production. The hybrid yeast, its Saccharomyces cerevisiae W303-1A G418R parent and Saccharomycodes ludwigii were not able to metabolise maltose during Durham tube tests. Schizosaccharomyces pombe, Lachancea fermentati and Pichia angusta metabolised maltose, however, showed limited ethanol production. Parameters, volatile and non-volatile organic compounds of beers produced by the studied yeast were analysed and compared to a beer produced by bottom fermented brewer’s yeast Saccharomyces pastorianus.
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Kelly, Amy C., and Reed B. Wickner. "Saccharomyces cerevisiae." Prion 7, no. 3 (May 2013): 215–20. http://dx.doi.org/10.4161/pri.24845.

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Elias-Arnanz, Montserrat, Antoine A. Firmenich, and P. Berg. "Saccharomyces cerevisiae." MGG Molecular & General Genetics 252, no. 5 (1996): 530. http://dx.doi.org/10.1007/s004380050260.

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Belda, Ignacio, Javier Ruiz, Antonio Santos, Nïel Van Wyk, and Isak S. Pretorius. "Saccharomyces cerevisiae." Trends in Genetics 35, no. 12 (December 2019): 956–57. http://dx.doi.org/10.1016/j.tig.2019.08.009.

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THAMMASITTIRONG, SUTTICHA NA-RANONG, THADA CHAMDUANG, UMAPORN PHONROD, and KLANARONG SRIROTH. "Ethanol Production Potential of Ethanol-Tolerant Saccharomyces and Non-Saccharomyces Yeasts." Polish Journal of Microbiology 61, no. 3 (2012): 219–21. http://dx.doi.org/10.33073/pjm-2012-029.

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Four ethanologenic ethanol-tolerant yeast strains, Saccharomyces cerevisiae (ATKU132), Saccharomycodes ludwigii (ATKU47), and Issatchenkia orientalis (ATKU5-60 and ATKU5-70), were isolated by an enrichment technique in yeast extract peptone dextrose (YPD) medium supplemented with 10% (v/v) ethanol at 30°C. Among non-Saccharomyces yeasts, Sd. ludwigii ATKU47 exhibited the highest ethanol-tolerance and ethanol production, which was similar to S. cerevisiae ATKU132. The maximum range of ethanol concentrations produced at 37°C by S. cerevisiae ATKU132 and Sd. ludwigii ATKU47 from an initial D-glucose concentration of 20% (w/v) and 28% (w/v) sugarcane molasses were 9.46-9.82% (w/v) and 8.07-8.32% (w/v), respectively.
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Wee, Hyun-Jeong, Sae-Byuk Lee, Kyu-Taek Choi, Ji-Yeon Ham, Soo-Hwan Yeo, and Heui-Dong Park. "Characteristics of freeze-concentrated apple cider fermented using mixed culture of non-Saccharomyces and Saccharomyces cerevisiae Fermivin." Korean Journal of Food Preservation 25, no. 6 (October 30, 2018): 730–41. http://dx.doi.org/10.11002/kjfp.2018.25.6.730.

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Zhang, Da Wei, Wenbin Dong, Lei Jin, Jie Zhang, and Yuan Chang Jin. "Isolation of Saccharomyces cerevisiae YDJ05 from the Spontaneous Fermentation Pear Wine and Study of the Yeast Growth Dynamics during the Association Fermentation." Advanced Materials Research 156-157 (October 2010): 266–71. http://dx.doi.org/10.4028/www.scientific.net/amr.156-157.266.

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Five preponderant yeast strains (YDJ01, YDJ02, YDJ03, YDJ04 and YDJ05) were isolated from the spontaneous fermentation pear wine as source of yeast for wine making from pear. Ethanol yield of YDJ05 was the highest and its using rapidity of the sugar was the most quickly. YDJ05 was identified as Saccharomyces cerevisiae and named Saccharomyces cerevisiae YDJ05. In addition, the fermentation dynamics of three yeast strains (Saccharomyces cerevisiae YDJ05, “Angle” yeast and Saccharomyces cerevisiae GIM2.39) were studied including single fermentation and associated fermentation. The fermentative behavior of three strains changed in association fermentations (Saccharomyces cerevisiae YDJ05 and “Angle” yeast, Saccharomyces cerevisiae YDJ05 and Saccharomyces cerevisiae GIM2.39). Results indicated that the qualities of pear wines made from association fermentations were better than that of single fermentations. The pear wine fermented associated by Saccharomyces cerevisiae YDJ05 and Saccharomyces cerevisiae GIM2.39 was the best in quality by sensory evaluation among all pear wines whose ethanol concentration was 10.3% (v/v). Saccharomyces cerevisiae YDJ05 and mai could be excellent potential source of strains.
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Musiyaka, V. K., A. A. Gladun, V. V. Sarnackaya, and R. I. Gvozdyak. "Antimutagenic activity of Saccharomyces cerevisiae strains." Biopolymers and Cell 16, no. 4 (July 20, 2000): 284–88. http://dx.doi.org/10.7124/bc.000573.

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Utama, Cahya Setya, Bambang Sulistiyanto, and Bhakti Etza Setiani. "Profil Mikrobiologis Pollard yang Difermentasi dengan Ekstrak Limbah Pasar Sayur pada Lama Peram yang Berbeda." Jurnal Agripet 13, no. 2 (October 1, 2013): 26–30. http://dx.doi.org/10.17969/agripet.v13i2.816.

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Profile microbiological of pollard fermented with extract of waste vegetable market in different long ripened ABSTRACT. The purpose of fermentation is to produce a product (material feed) that have nutritional content, texture and better biological availability, while it also can reduce the anti-nutritional. Microorganisms are often used as probiotics in feed is kind of Lactobacillus sp and Saccharomyces cerevisiae. Microorganisms are able to produce secondary metabolites such as β -glucan, mannan oligosaccharides and anti-cancer. Very familier as probiotic Lactobacillus among humans or livestock , while saccharomyces cerevisiae have specific characteristics in animal feed because of its ability to produce glutamic acid which can increase feed palatability. Grant Saccharomyces cerevisie can enhance digest protein and fiber, such as cellulose and hemicellulose , with Sacaromyces cerevisiea supplementation can increase the rate of short-chain fatty acids in cecum and suppresses the growth of bacteria from the Enterobacteriaceae species. Observing the above, needed an activity to find additional engineering efforts antibiotics as a source of natural probiotic , prebiotic and synbiotic on the particular poultry and livestock in general, to take advantage of the waste as a probiotic supplement that naturally produced feed additives to support healthy organic livestock production and economically.
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Dissertations / Theses on the topic "Saccharomyces cerevisiae"

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Schorling, Stefan. "Ceramidsynthese in Saccharomyces cerevisiae." Diss., lmu, 2001. http://nbn-resolving.de/urn:nbn:de:bvb:19-3658.

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Deans, Karen. "Ageing of Saccharomyces cerevisiae." Thesis, Heriot-Watt University, 1997. http://hdl.handle.net/10399/663.

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Ericson, Elke. "High-resolution phenomics to decode : yeast stress physiology /." Göteborg : Göteborg University, Dept. of Cell and Molecular Biology, Faculty of Science, 2006. http://www.loc.gov/catdir/toc/fy0707/2006436807.html.

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Eriksson, Peter. "Identification of the two GPD isogenes of saccharomyces cerevisiae and characterization of their response to hyper-osmotic stress." Göteborg : Chalmers Reproservice, 1996. http://catalog.hathitrust.org/api/volumes/oclc/38202006.html.

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Pratt, Elizabeth Stratton. "Genetic and biochemical studies of Adr6, a component of the SWI/SNF chromatin remodeling complex /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/10288.

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Kerkmann, Katja. "Die genomweite Expressionsanalyse von Deletionsmutanten der Gene NHP6A/B und CDC73 in der Hefe S.cerevisiae." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961961651.

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Bellahn, Inga. "Biochemische Charakterisierung vakuolärer Vesikel aus Saccharomyces cerevisiae." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=965643484.

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Jestel, Anja. "Strukturelle Charakterisierung des Calpastatin und Untersuchung eines ATP-abhängigen Peptidtransports in S. cerevisiae." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=966507193.

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Schauen, Matthias. "Mitochondriale Transportproteine in Saccharomyces cerevisiae." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=965029379.

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Schulze, Ulrik. "Anaerobic physiology of Saccharomyces cerevisiae /." Online version, 1995. http://bibpurl.oclc.org/web/20903.

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Books on the topic "Saccharomyces cerevisiae"

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Mojzita, Dominik. Thiamine-related regulation of metabolism and gene expression in the yeast Saccharomyces cerevisiae. Göteborg: Dept. of Cellular and Molecular Biology, Göteborg University, 2007.

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Pettersson, Nina. Functional analysis of aquaporins Saccharomyces cerevisae. Göteborg: Department of Cell and Molecular Biology, Göteborg University, 2005.

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Pettersson, Nina. Functional analysis of aquaporins Saccharomyces cerevisae. Göteborg: Department of Cell and Molecular Biology, Göteborg University, 2005.

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Wingler, Laura Michele. Harnessing Saccharomyces cerevisiae Genetics for Cell Engineering. [New York, N.Y.?]: [publisher not identified], 2011.

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Smart, Christopher Andrew. Biotransformations of ketoximes by saccharomyces cerevisiae NCYC 1765. [s.l.]: typescript, 1995.

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Chan, Helen G. Y. The Effects of chemotherapeutic drugs on saccharomyces cerevisiae. Sudbury, Ont: Laurentian University, 1997.

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Заенфелд, Г. К. Иммунологический механизм действия полисахаридов дрожжевых клеток Saccharomyces cerevisiae. Рига: Зинатне, 1990.

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Hill, James. Genetic manipulation and biochemical studies of Saccharomyces Cerevisiae. [s.l.]: typescript, 1991.

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Richard, Dickinson J., and Schweizer Michael 1947-, eds. The metabolism and molecular physiology of Saccharomyces cerevisiae. London: Taylor & Francis, 1999.

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Mortimer, Robert K. Genetic map of Saccharomyces cerevisiae: (as of November 1984). [Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory], 1985.

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Book chapters on the topic "Saccharomyces cerevisiae"

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Friedberg, Errol C., William J. Feaver, Wenya Huang, Michael S. Reagan, Simon H. Reed, Zhaoyang You, Shuguang Wei, Karl Rodriguez, Jose Talamantez, and Alan E. Tomkinson. "Saccharomyces Cerevisiae." In Advances in DNA Damage and Repair, 111–23. Boston, MA: Springer US, 1999. http://dx.doi.org/10.1007/978-1-4615-4865-2_10.

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Hooykaas, Paul J. J., Amke Dulk-Ras, Paul Bundock, Jalal Soltani, Haico Attikum, and G. Paul H. Heusden. "Yeast (Saccharomyces cerevisiae)." In Agrobacterium Protocols Volume 2, 465–73. Totowa, NJ: Humana Press, 2006. http://dx.doi.org/10.1385/1-59745-131-2:465.

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Kunau, Wolf H., and Andreas Hartig. "Peroxisome biogenesis in Saccharomyces cerevisiae." In Molecular Biology of Saccharomyces, 63–78. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2504-8_6.

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Wang, Xinping, Yinglin Bai, Li Ni, and Henry Weiner. "Saccharomyces cerevisiae Aldehyde Dehydrogenases." In Advances in Experimental Medicine and Biology, 277–80. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4615-5871-2_32.

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Meilhoc, E., and J. Teissie. "Electrotransformation of Saccharomyces cerevisiae." In Methods in Molecular Biology, 187–93. New York, NY: Springer US, 2019. http://dx.doi.org/10.1007/978-1-4939-9740-4_21.

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Stöcker, W. "Antikörper gegen Saccharomyces cerevisiae." In Lexikon der Medizinischen Laboratoriumsdiagnostik, 1–2. Berlin, Heidelberg: Springer Berlin Heidelberg, 2017. http://dx.doi.org/10.1007/978-3-662-49054-9_236-1.

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Stöcker, W. "Antikörper gegen Saccharomyces cerevisiae." In Springer Reference Medizin, 159–60. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_236.

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Geijer, Cecilia, Daphna Joseph-Strauss, Giora Simchen, Naama Barkai, and Stefan Hohmann. "Saccharomyces cerevisiae Spore Germination." In Dormancy and Resistance in Harsh Environments, 29–41. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-12422-8_3.

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Konopka, James B., and Stanley Fields. "The pheromone signal pathway in Saccharomyces cerevisiae." In Molecular Biology of Saccharomyces, 95–108. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2504-8_8.

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Longo, Valter D., and Paola Fabrizio. "Chronological Aging in Saccharomyces cerevisiae." In Aging Research in Yeast, 101–21. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-2561-4_5.

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Conference papers on the topic "Saccharomyces cerevisiae"

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Heath, Allison P., Lydia Kavraki, and Gabor Balazsi. "Bipolarity of the Saccharomyces Cerevisiae Genome." In 2008 2nd International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2008. http://dx.doi.org/10.1109/icbbe.2008.84.

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Yang, Yueying, Di Liu, and Jun Meng. "Module of cellular networks in saccharomyces cerevisiae." In 2012 IEEE 6th International Conference on Systems Biology (ISB). IEEE, 2012. http://dx.doi.org/10.1109/isb.2012.6314133.

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Ragothaman Avanasi Narasimhan, Ganti S Murthy, and Christopher Beatty. "Hemicellulose fermentation by industrial yeast Saccharomyces cerevisiae." In 2010 Pittsburgh, Pennsylvania, June 20 - June 23, 2010. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2010. http://dx.doi.org/10.13031/2013.29920.

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Борисенко, О. А. "Влияние холодного охмеления на дрожжи Saccharomyces cerevisiae." In Наука России: Цели и задачи. НИЦ "LJournal", 2021. http://dx.doi.org/10.18411/sr-10-06-2021-39.

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В представленной работе исследуется влияние холодного охмеления на физиологическое состояние дрожжей. В процессе исследований проводились модельные опыты по холодному охмелению при температуре 5°С и 20°С с применением хмеля Магнум и Тетнангер и двух рас пивоваренных дрожжей Saccharomyces cerevisiae: Rh – низового брожения и Nottingham (Nt) – верхового брожения. Показано, что условия холодного охмеления одинаково воздействуют на дрожжи низового и верхового брожения с точки зрения влияния на физиологическое состояние и критическое влияние на процесс оказывает температура. Выявлено положительное влияние пониженных температур на жизнедеятельность дрожжей и их физиологическое состояние.
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Yang, Chenyu, and Yilin Li. "Gene Editing of Saccharomyces Cerevisiae Using CRISPR." In International Conference on Biotechnology and Biomedicine. SCITEPRESS - Science and Technology Publications, 2022. http://dx.doi.org/10.5220/0012021800003633.

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Gabrovšek, Ana, Nika Tašler, Rigoberto Barrios-Francisco, and Marko Jeran. "Impact of a Saccharin Higher Homolog on Saccharomyces cerevisiae." In Socratic Lectures 7. University of Lubljana Press, 2022. http://dx.doi.org/10.55295/psl.2022.d15.

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Saccharin is an organic compound, which is often used as a calorie-free artificial sweetener. It salts are being produced for the market for over 80 years. Saccharin and its derivates are very applicatory oriented, therefore researchers synthesize more and more active ingredients, which could potentially show better performance. This work considers the effect of biological activity of a newly synthesized saccharin derivative Me- thyl 4-hydroxy-1,1-dioxo-2H-1,2-benzothiazine-3-carboxylate (6Sac) on yeast Saccharomyces cere-visiae. Qualitative comparison of the studied activity with the activity of the saccharine sodium salt is presented. Our results were gained by two different ways of viability detection: counting dead/live cells dyed with methylene blue and counting colony-forming units (CFU). The study has shown that the saccharine derivative with an ester functional group has negative effect on growth and repro-duction of yeast. The qualitative comparison of the activity of the tested substance with the already known activity of saccharine sodium salt is a convenient method for following the model organism Saccharomyces cerevisiae. Keywords: Saccharin, sodium saccharinate, Saccharomyces cerevisiae, Viability, Methylene blue, Col-ony-forming units (CFU), Medicine
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Silva, Luana Caroline Domingos Da, and Vivianne Lúcia Bormann De Souza. "EFEITO DA RADIAÇÃO IONIZANTE EM SOLUÇÕES CONTENDO SACCHAROMYCES CEREVISIAE." In II Congresso Brasileiro de Biotecnologia On-line. Revista Multidisciplinar de Educação e Meio Ambiente, 2022. http://dx.doi.org/10.51189/conbiotec/16.

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Introdução: A busca por potencializar atividades biológicas com tecnologias diversas tem sido bastante aplicada, incluindo o uso de radiações, que de acordo com a dose empregada influencia na propriedade antimicrobiana. O microrganismo Saccharomyces cerevisiae é aeróbio facultativo, isto é, tem a habilidade de se ajustar metabolicamente, tanto em condições de aerobiose como de anaerobiose. E, sabe-se que a irradiação é capaz de causar danos ao DNA da célula, e alterações em seus produtos finais, que podem ser positivas no sentido de produzir substâncias biologicamente ativas, ou substâncias prejudiciais à saúde humana, com isso é importante averiguar os produtos finais produzidos após a irradiação da Saccharomyces cerevisiae. Objetivo: Analisar estudos recentes e métodos utilizados relacionados a Saccharomyces cerevisiae e a irradiação. Metodologia: A estratégia deste tratou-se de uma revisão sistemática da literatura desenvolvida com o propósito de contribuir para o conhecimento, desenvolvido em cinco etapas: busca da literatura, extração de dados, avaliação dos estudos encontrados, análise e síntese dos resultados. Para condução do estudo, a pesquisa foi realizada entre os meses de maio a junho de 2021, nas bases de dados: Scielo, Brazilian Journal of Development e a Revista Brasileira de Produtos Agroindustriais. Teve como critério de inclusão os artigos relacionados a radiação ionizante e o microrganismo cerevisiae, referente aos últimos 10 anos de publicação e os critérios de exclusão foram produções científicas em formato de tese, dissertação e estudo do caso. Resultados: Foram analisados 3 trabalhos, e cada um desses mostra que a irradiação no microrganismo com a radiação gama, alteração de temperatura e agitação, além da ação da luz branca e a luz UV com doses médias e altas são eficazes na eliminação de microrganismos. Conclusão: Em alguns experimentos realizados pela nossa equipe já se identifica que a radiação reduzia a quantidade de microrganismos, e esses resultados corroboram com os 3 trabalhos analisados. Assim, pode-se afirmar que a radiação pode eliminar microrganismos e age também de forma a torna-lo estático, entretanto ainda estão sendo realizados novos experimentos para verificar possíveis mudanças causadas no microrganismo.
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Dong, Limin, Zhuo Diao, Juan Du, Zhao Jiang, Qingjuan Meng, and Ying Zhang. "Mechanism of Cu(II) Biosorption by Saccharomyces Cerevisiae." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5163036.

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Limin, Dong, Du Juan, Bai Xin, Yu Naili, Fan Chunhui, and Zhang Ying. "Mechanism of Pb(II) Biosorption by Saccharomyces Cerevisiae." In 2009 International Conference on Environmental Science and Information Application Technology, ESIAT. IEEE, 2009. http://dx.doi.org/10.1109/esiat.2009.450.

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Raffar, Nur Izzati Abdul, Nadhratul Nur Ain Abdul Rahman, Rasyidah Alrozi, Faraziehan Senusi, and Siu Hua Chang. "Potential immobilized Saccharomyces cerevisiae as heavy metal removal." In INTERNATIONAL CONFERENCE ON MATHEMATICS, ENGINEERING AND INDUSTRIAL APPLICATIONS 2014 (ICoMEIA 2014). AIP Publishing LLC, 2015. http://dx.doi.org/10.1063/1.4915810.

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Reports on the topic "Saccharomyces cerevisiae"

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DeLoache, William, Zachary Russ, Jennifer Samson, and John Dueber. Repurposing the Saccharomyces cerevisiae peroxisome for compartmentalizing multi-enzyme pathways. Office of Scientific and Technical Information (OSTI), September 2017. http://dx.doi.org/10.2172/1394729.

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Campbell, Chelsea, Cullen Horstmann, Kyoungtae Kim, and Alan Kennedy. Saccharomyces cerevisiae (Budding Yeast); Standard Operating Procedure Series : Toxicology (T). Engineer Research and Development Center (U.S.), August 2019. http://dx.doi.org/10.21079/11681/33688.

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Turner, Joshua, Lizabeth Thomas, and Sarah Kennedy. Structural Analysis of a New Saccharomyces cerevisiae α-glucosidase Homology Model and Identification of Potential Inhibitor Enzyme Docking Sites. Journal of Young Investigators, October 2020. http://dx.doi.org/10.22186/jyi.38.4.27-33.

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Alexandar, Irina, Diana Zasheva, and Nikolay Kaloyanov. Antimicrobial Activity of New Molecular Complexes of 1,10‑Phenanthroline and 5‑Amino‑1,10‑Phenanthroline on Escherichia coli and Saccharomyces cerevisiae Strains. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, February 2019. http://dx.doi.org/10.7546/crabs.2019.01.10.

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Zhao, Chun. Suppressors (scsl-scs7) of CSG2, a Gene Required by Saccharomyces cerevisiae for Growth in Media Containing 10 mMCa(2+), Identify Genes Required for Sphingolipid Biosynthesis. Fort Belvoir, VA: Defense Technical Information Center, June 1994. http://dx.doi.org/10.21236/ad1011395.

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Luther, Jamie, Holly Goodson, and Clint Arnett. Development of a genetic memory platform for detection of metals in water : use of mRNA and protein destabilization elements as a means to control autoinduction from the CUP1 promoter of Saccharomyces cerevisiae. Construction Engineering Research Laboratory (U.S.), June 2018. http://dx.doi.org/10.21079/11681/27275.

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Droby, Samir, Joseph W. Eckert, Shulamit Manulis, and Rajesh K. Mehra. Ecology, Population Dynamics and Genetic Diversity of Epiphytic Yeast Antagonists of Postharvest Diseases of Fruits. United States Department of Agriculture, October 1994. http://dx.doi.org/10.32747/1994.7568777.bard.

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One of the emerging technologies is the use of microbial agents for the control of postharvest diseases of fruits and vegetables. A number of antagonistic microorganisms have been discovered which have the potential to effectively control postharvest diseases. Some of this technology has been patented and commercial products such as AspireTM (Ecogen Corporatin, Langhorne, PA, USA), Biosave 10TM and Biosave 11TM (Ecoscience Inc., Worchester, MA, USA) have been registered for commercial use. The principal investigator of this project was involved in developing the yeast-based biofungicide-AspireTM and testing its efficacy under commercial conditions. This research project was initiated to fill the gap between the knowledge available on development and commercial implementation of yeast biocontrol agents and basic understanding of various aspects related to introducing yeast antagonists to fruit surfaces, along with verification of population genetics. The main objectives of this study were: Study ecology, population dynamics and genetic diversity of the yeast antagonists Candida guilliermondii, C. oleophila, and Debaryomyces hansenii, and study the effect of preharvest application of the yeast antagonist C. oleophila naturally occurring epiphytic microbial population and on the development of postharvest diseases of citrus fruit during storage. Our findings, which were detailed in several publications, have shown that an epiphytic yeast population of grapefruit able to grow under high osmotic conditions and a wide range of temperatures was isolated and characterized for its biocontrol activity against green mold decay caused by Penicillium digitatum. Techniques based on random amplified polymorphic DNA (RAPD) and arbitrary primed polymerase chain reaction (ap-PCR), as well as homologies between sequences of the rDNA internal transcribed spacers (ITS) and 5.8S gene, were used to characterize the composition of the yeast population and to determine the genetic relationship among predominant yeast species. Epiphytic yeasts exhibiting the highest biocontrol activity against P. digitatum on grapefruit were identified as Candida guilliermondii, C. oleophila, C. sake, and Debaryomyces hansenii, while C. guilliermondii was the most predominant species. RAPD and ap-PCR analysis of the osmotolerant yeast population showed two different, major groups. The sequences of the ITS regions and the 5.8S gene of the yeast isolates, previously identified as belonging to different species, were found to be identical. Following the need to develop a genetically marked strain of the yeast C. oleophila, to be used in population dynamics studies, a transformation system for the yeast was developed. Histidine auxotrophy of C. oloephila produced using ethyl methanesulfonate were transformed with plasmids containing HIS3, HIS4 and HIS5 genes from Saccharomyces cerevisiae. In one mutant histidin auxotrophy was complemented by the HIS5 gene of S. cerevisiae is functionally homologous to the HIS5 gene in V. oleophila. Southern blot analysis showed that the plasmid containing the S. cerevisiae HIS5 gene was integrated at a different location every C. oleophila HIS+ transformant. There were no detectable physiological differences between C. oleophila strain I-182 and the transformants. The biological control ability of C. oleophila was not affected by the transformation. A genetically marked (with b-glucuronidase gene) transformant of C. oleophila colonized wounds on orange fruits and its population increased under field conditions. Effect of preharvest application of the yeast C. oleophila on population dynamics of epiphytic microbial population on wounded and unwounded grapefruit surface in the orchard and after harvest was also studied. In addition, the effect of preharvest application of the yeast C. oleophila on the development of postharvest decay was evaluated. Population studies conducted in the orchard showed that in control, non-treated fruit, colonization of wounded and unwounded grapefruit surface by naturally occurring filamentous fungi did not vary throughout the incubation period on the tree. On the other hand, colonization of intact and wounded fruit surface by naturally occurring yeasts was different. Yeasts colonized wounded surface rapidly and increased in numbers to about two orders of magnitude as compared to unwounded surface. On fruit treated with the yeast and kept on the tree, a different picture of fungal and yeast population had emerged. The detected fungal population on the yeast-treated intact surface was dramatically reduced and in treated wounds no fungi was detected. Yeast population on intact surface was relatively high immediately after the application of AspireTM and decreased to than 70% of that detected initially. In wounds, yeast population increased from 2.5 x 104 to about 4x106 after 72 hours of incubation at 20oC. Results of tests conducted to evaluate the effect of preharvest application of AspireTM on the development of postharvest decay indicated the validity of the approach.
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Shapira, Roni, Judith Grizzle, Nachman Paster, Mark Pines, and Chamindrani Mendis-Handagama. Novel Approach to Mycotoxin Detoxification in Farm Animals Using Probiotics Added to Feed Stuffs. United States Department of Agriculture, May 2010. http://dx.doi.org/10.32747/2010.7592115.bard.

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T-2 toxin, a toxic product belongs to the trichothecene mycotoxins, attracts major interest because of its severe detrimental effects on the health of human and farm animals. The occurrence of trichothecenes contamination is global and they are very resistant to physical or chemical detoxification techniques. Trichothecenes are absorbed in the small intestine into the blood stream. The hypothesis of this project was to develop a protecting system using probiotic bacteria that will express trichothecene 3-O-acetyltransferase (Tri101) that convert T-2 to a less toxic intermediate to reduce ingested levels in-situ. The major obstacle that we had faced during the project is the absence of stable and efficient expression vectors in probiotics. Most of the project period was invested to screen and isolate strong promoter to express high amounts of the detoxify enzyme on one hand and to stabilize the expression vector on the other hand. In order to estimate the detoxification capacity of the isolated promoters we had developed two very sensitive bioassays.The first system was based on Saccharomyces cerevisiae cells expressing the green fluorescent protein (GFP). Human liver cells proliferation was used as the second bioassay system.Using both systems we were able to prove actual detoxification on living cells by probiotic bacteria expressing Tri101. The first step was the isolation of already discovered strong promoters from lactic acid bacteria, cloning them downstream the Tri101 gene and transformed vectors to E. coli, a lactic acid bacteria strain Lactococcuslactis MG1363, and a probiotic strain of Lactobacillus casei. All plasmid constructs transformed to L. casei were unstable. The promoter designated lacA found to be the most efficient in reducing T-2 from the growth media of E. coli and L. lactis. A prompter library was generated from L. casei in order to isolate authentic probiotic promoters. Seven promoters were isolated, cloned downstream Tri101, transformed to bacteria and their detoxification capability was compared. One of those prompters, designated P201 showed a relatively high efficiency in detoxification. Sequence analysis of the promoter region of P201 and another promoter, P41, revealed the consensus region recognized by the sigma factor. We further attempted to isolate an inducible, strong promoter by comparing the protein profiles of L. casei grown in the presence of 0.3% bile salt (mimicking intestine conditions). Six spots that were consistently overexpressed in the presence of bile salts were isolated and identified. Their promoter reigns are now under investigation and characterization.
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Zhou, Ting, Roni Shapira, Peter Pauls, Nachman Paster, and Mark Pines. Biological Detoxification of the Mycotoxin Deoxynivalenol (DON) to Improve Safety of Animal Feed and Food. United States Department of Agriculture, July 2010. http://dx.doi.org/10.32747/2010.7613885.bard.

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The trichothecene deoxynivalenol (DON, vomitoxin), one of the most common mycotoxin contaminants of grains, is produced by members of the Fusarium genus. DON poses a health risk to consumers and impairs livestock performance because it causes feed refusal, nausea, vomiting, diarrhea, hemolytic effects and cellular injury. The occurrence of trichothecenes contamination is global and they are very resistant to physical or chemical detoxification techniques. Trichothecenes are absorbed in the small intestine into the blood stream. The overall objective of this project was to develop a protecting system using probiotic bacteria that will express trichothecene 3-O-acetyltransferase (Tri101) that convert T-2 to a less toxic intermediate to reduce ingested levels in-situ. The major obstacle that we had faced during the project is the absence of stable and efficient expression vectors in probiotics. Most of the project period was invested to screen and isolate strong promoter to express high amounts of the detoxify enzyme on one hand and to stabilize the expression vector on the other hand. In order to estimate the detoxification capacity of the isolated promoters we had developed two very sensitive bioassays.The first system was based on Saccharomyces cerevisiae cells expressing the green fluorescent protein (GFP). Human liver cells proliferation was used as the second bioassay system.Using both systems we were able to prove actual detoxification on living cells by probiotic bacteria expressing Tri101. The first step was the isolation of already discovered strong promoters from lactic acid bacteria, cloning them downstream the Tri101 gene and transformed vectors to E. coli, a lactic acid bacteria strain Lactococcuslactis MG1363, and a probiotic strain of Lactobacillus casei. All plasmid constructs transformed to L. casei were unstable. The promoter designated lacA found to be the most efficient in reducing T-2 from the growth media of E. coli and L. lactis. A prompter library was generated from L. casei in order to isolate authentic probiotic promoters. Seven promoters were isolated, cloned downstream Tri101, transformed to bacteria and their detoxification capability was compared. One of those prompters, designated P201 showed a relatively high efficiency in detoxification. Sequence analysis of the promoter region of P201 and another promoter, P41, revealed the consensus region recognized by the sigma factor. We further attempted to isolate an inducible, strong promoter by comparing the protein profiles of L. casei grown in the presence of 0.3% bile salt (mimicking intestine conditions). Six spots that were consistently overexpressed in the presence of bile salts were isolated and identified. Their promoter reigns are now under investigation and characterization.
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Irudayaraj, Joseph, Ze'ev Schmilovitch, Amos Mizrach, Giora Kritzman, and Chitrita DebRoy. Rapid detection of food borne pathogens and non-pathogens in fresh produce using FT-IRS and raman spectroscopy. United States Department of Agriculture, October 2004. http://dx.doi.org/10.32747/2004.7587221.bard.

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Rapid detection of pathogens and hazardous elements in fresh fruits and vegetables after harvest requires the use of advanced sensor technology at each step in the farm-to-consumer or farm-to-processing sequence. Fourier-transform infrared (FTIR) spectroscopy and the complementary Raman spectroscopy, an advanced optical technique based on light scattering will be investigated for rapid and on-site assessment of produce safety. Paving the way toward the development of this innovative methodology, specific original objectives were to (1) identify and distinguish different serotypes of Escherichia coli, Listeria monocytogenes, Salmonella typhimurium, and Bacillus cereus by FTIR and Raman spectroscopy, (2) develop spectroscopic fingerprint patterns and detection methodology for fungi such as Aspergillus, Rhizopus, Fusarium, and Penicillium (3) to validate a universal spectroscopic procedure to detect foodborne pathogens and non-pathogens in food systems. The original objectives proposed were very ambitious hence modifications were necessary to fit with the funding. Elaborate experiments were conducted for sensitivity, additionally, testing a wide range of pathogens (more than selected list proposed) was also necessary to demonstrate the robustness of the instruments, most crucially, algorithms for differentiating a specific organism of interest in mixed cultures was conceptualized and validated, and finally neural network and chemometric models were tested on a variety of applications. Food systems tested were apple juice and buffer systems. Pathogens tested include Enterococcus faecium, Salmonella enteritidis, Salmonella typhimurium, Bacillus cereus, Yersinia enterocolitis, Shigella boydii, Staphylococus aureus, Serratiamarcescens, Pseudomonas vulgaris, Vibrio cholerae, Hafniaalvei, Enterobacter cloacae, Enterobacter aerogenes, E. coli (O103, O55, O121, O30 and O26), Aspergillus niger (NRRL 326) and Fusarium verticilliodes (NRRL 13586), Saccharomyces cerevisiae (ATCC 24859), Lactobacillus casei (ATCC 11443), Erwinia carotovora pv. carotovora and Clavibacter michiganense. Sensitivity of the FTIR detection was 103CFU/ml and a clear differentiation was obtained between the different organisms both at the species as well as at the strain level for the tested pathogens. A very crucial step in the direction of analyzing mixed cultures was taken. The vector based algorithm was able to identify a target pathogen of interest in a mixture of up to three organisms. Efforts will be made to extend this to 10-12 key pathogens. The experience gained was very helpful in laying the foundations for extracting the true fingerprint of a specific pathogen irrespective of the background substrate. This is very crucial especially when experimenting with solid samples as well as complex food matrices. Spectroscopic techniques, especially FTIR and Raman methods are being pursued by agencies such as DARPA and Department of Defense to combat homeland security. Through the BARD US-3296-02 feasibility grant, the foundations for detection, sample handling, and the needed algorithms and models were developed. Successive efforts will be made in transferring the methodology to fruit surfaces and to other complex food matrices which can be accomplished with creative sampling methods and experimentation. Even a marginal success in this direction will result in a very significant breakthrough because FTIR and Raman methods, in spite of their limitations are still one of most rapid and nondestructive methods available. Continued interest and efforts in improving the components as well as the refinement of the procedures is bound to result in a significant breakthrough in sensor technology for food safety and biosecurity.
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