Dissertations / Theses on the topic 'RUNX'
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LeBlanc, Kimberly T. "Runx Expression in Normal and Osteoarthritic Cartilage: Possible Functions of Runx Proteins in Chondrocytes: A Dissertation." eScholarship@UMMS, 2013. https://escholarship.umassmed.edu/gsbs_diss/655.
Full textLeBlanc, Kimberly T. "Runx Expression in Normal and Osteoarthritic Cartilage: Possible Functions of Runx Proteins in Chondrocytes: A Dissertation." eScholarship@UMMS, 2002. http://escholarship.umassmed.edu/gsbs_diss/655.
Full textWhiteman, Hannah Juliette. "RUNX expression and function in human B cells." Thesis, Imperial College London, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.422343.
Full textChen, Aichun. "Regulation of lozenge transcription factor activity and blood cell development by MLF and its partner DnaJ-1." Thesis, Toulouse 3, 2017. http://www.theses.fr/2017TOU30064/document.
Full textHematopoiesis is the process of formation of fully differentiated blood cells from hematopoietic stem cells (HSCs). This process is tightly controlled by the integration of developmental and homeostatic signals to ensure the generation of an appropriate number of each blood cell type. At the molecular level, the regulation of this developmental process is mediated by a number of transcription factors, especially by members of the RUNX family, and mutations affecting these factors are at the origin of numerous hemopathies, including leukemia. Intriguingly, many transcriptional regulators and signaling pathways controlling blood cell development are evolutionarily conserved from humans to Drosophila melanogaster. Hence, the fruit fly has become a potent and simplified model to study the mechanisms underlying the specification of blood cell lineages and the regulation of blood cell homeostasis. Members of the Myeloid Leukemia Factor (MLF) family have been implicated in hematopoiesis and in oncogenic blood cell transformation, but their function and molecular mechanism of action remain elusive. Previous work in Drosophila showed that MLF stabilizes the RUNX transcription factor Lozenge (LZ) and controls the number of LZ+ blood cells. During my PhD, I sought to further decipher the molecular mechanism of action of MLF on Lozenge during blood cell development. Using a proteomic approach in Drosophila Kc167 cells, we identified the Hsp40 co-chaperone family member DnaJ-1 and its chaperone partner Hsc70-4 as two partners of MLF. These interactions were confirmed by co-immunoprecipitations and in vitro pull-down assays. Importantly, we found that knocking down DnaJ-1 or Hsc70-4 expression in Kc167 cells caused a reduction in the level of Lozenge protein and a concomitant decrease in Lozenge transactivation activity, which were very similar to those caused by MLF knock-down. Similarly, over-expression of two DnaJ-1 mutants that are unable to stimulate the chaperone activity of Hsc70-4 also decreased Lozenge level and impaired its capacity to activate transcription. These results suggest that MLF could act within a chaperone complex composed of DnaJ-1 and Hsc70-4 to control Lozenge stability and activity. Along that line, we showed by co-immunoprecipitation that Lozenge interacts with MLF, DnaJ-1 and Hsc70-4, respectively. Using various truncated mutants of MLF or DnaJ-1, we showed that MLF and DnaJ-1 interact and together with Lozenge through their conserved MLF homology domain (MHD) and C-terminal region, respectively. Furthermore, in vitro GST pull-down assays suggested that the interactions between MLF, DnaJ-1 and Lozenge are direct. Thus, we propose that MLF and DnaJ-1 control Lozenge protein level by interacting with it and by promoting its folding and/or solubility via the Hsc70 chaperone machinery. In parallel, we assessed DnaJ-1 function in Drosophila blood cells in vivo using a null allele of dnaj-1 generated by CRISPR/Cas9 technique. We found that, like mlf, dnaj-1 mutation leads to an increase in the number and size of LZ+ blood cells, as well as to an over-activation of the Notch signaling pathway in these cells. Moreover, our data suggested that high levels of active Lozenge are required to control the number and size of LZ+ blood cells, and to down-regulate Notch expression. We propose that the MLF/DnaJ-1 complex controls LZ+ blood cell development in vivo by regulating Lozenge protein level/activity and thereby Notch pathway activation. In sum, our results establish a functional link between MLF, the Hsp40 co-chaperone DnaJ-1 and the RUNX transcription factor Lozenge, which could be conserved in other species
Antony-Debré, Iléana. "Fpd/aml : diagnostic et modélisation d'anomalies de Runx 1." Paris 7, 2013. http://www.theses.fr/2013PA077049.
Full textFPD/AML (familial platelet disorder with predisposition to acute myeloid leukemia) results from constitutive alterations of RUNX1, a hematopoietic transcription factor essential to definitive hematopoiesis. In the first part of our work, we proposed a new diagnostic test to detect RUNX1 alterations. We showed that MYH10, which is regulated negatively by RUNX1 during megakaryopoiesis, persisted in platelets from FPD/AML patients. MYHIO persistence was not detected in platelets from patients with other constitutional thrombocytopenia, except from patients with Paris-Trousseau syndrome. This new test could be used also to detect RUNX1 alterations in acquired haematological disorders like chronic myelomonocytic leukemia. In a second work, we generated induced pluripotent stem cells (iPSC) from fibroblasts of FPD/AML patients with different RUNX1 alterations, in order to model the pathology. We reproduced the phenotype already described in patients with defect in megakaryocytic lineage whatever RUNX1 alterations and increase in granulo-monocytic compartment only with the mutation which predisposes to leukemia. We highlighted for the first time that RUNX1 is necessary also for erythroid lineage. We confirmed these results after RUNX1 knock down in an embryonic stem cell line. In conclusion we validated our model and now we can use it to study the mechanisms leading to dysmegakaryopoiesis and predisposition to leukemia in FPD/AML patients
Pande, Sandhya. "Regulation of Runx Proteins in Human Cancers: A Dissertation." eScholarship@UMMS, 2011. https://escholarship.umassmed.edu/gsbs_diss/559.
Full textStephens, Alexandre, and N/A. "Genetic and Functional Characterization of RUNX2." Griffith University. School of Medical Science, 2007. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20070823.100953.
Full textStephens, Alexandre. "Genetic and Functional Characterization of RUNX2." Thesis, Griffith University, 2007. http://hdl.handle.net/10072/365677.
Full textThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Medical Science
Faculty of Health
Full Text
Ferguson, Alison Mary. "The Role of RUNX Transcription Factors in Prostate Development and Tumorigenesis." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/16898.
Full textFerrari, Nicola. "Investigating RUNX transcription factors in mammary gland development and breast cancer." Thesis, University of Glasgow, 2013. http://theses.gla.ac.uk/4790/.
Full textMcLarren, Keith W. "Characterizing the physical and functional interactions between RUNX, Hes1 and TLE proteins." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=38233.
Full textIt is shown here that mammalian Hes1 requires TLEs to repress gene expression, similar to the requirement Drosophila Hes proteins have for Groucho. It is shown further that TLEs also interact with mammalian RUNX proteins and inhibit RUNX's ability to transactivate gene expression. In contrast, Hes1 also physically associates with RUNX and enhances RUNX-mediated transactivation. These results, together with our additional finding that RUNX can inhibit Hes1-mediated transcriptional repression, suggest for the first time that RUNX and Hes1 proteins regulate each other's transcriptional functions.
This thesis also shows that Hes1 can be found in a discreet nuclear subcompartment involved in the regulation of gene expression, termed the nuclear matrix. Nuclear matrix binding is mediated by the same Hes1 C-terminal domain that acts as a binding site for Groucho/TLEs, which also localize to the nuclear matrix and are required for Hes1-mediated transcriptional repression. Both the nuclear matrix association and transcription repression activity of Hes1 are inhibited by deletion of its C-terminal region, indicating that Groucho/TLEs act as transcriptional corepressors for Hes1.
Together, the material presented in this thesis suggests that Hes1 and RUNX proteins interact with each other to modulate gene expression in the development of a number of tissues.
Ran, Ran. "RUNX transcription factors drive epithelial to mesenchymal transition in metastatic breast cancer cells." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/runx-transcription-factors-drive-epithelial-to-mesenchymal-transition-in-metastatic-breast-cancer-cells(224fac5a-0188-4dd5-8c20-b1749fbbc32d).html.
Full textViscelli, Bruno Alvares. "Estudos dos efeitos de um anti-inflamatório não esteroidal seletivo para COX-2 na osteogênese e na expressão das proteínas COX-2 e RUNX-2 durante o reparo ósseo alveolar em ratos." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/25/25149/tde-01112012-180557/.
Full textObjective: To evaluate morphometrically possible changes in post-extraction alveolar bone healing in rats treated with Meloxicam, a selective anti-inflammatory inhibitor of cyclooxygenase (COX-2) and to correlate it with the temporal expression of COX-2 and transcription factor 2 with Runt domain (Runx-2) associated with differentiation of osteoblastic lineage cells. Material and Methods: The extraction of the right upper incisor was made in 120 male Wistar rats, divided into control group (n=60) - animals treated with an intraperitoneal injection of 0,1 ml of 0,9% NaCl solution daily for 7 days and the treated group (n=60) - animals treated with injection of 3mg/kg of body weight of Meloxicam 0.9% NaCl solution daily for 7 days. The total alveolar volume (VtA), total bone tissue volume (VtO), number of immunohistochemically positive cells/mm² (Nm) for COX-2 and RUNX-2 and the Western blotting (WB) COX-2 and RUNX-2 protein expressions were evaluated after 3, 7, 10, 14, 21 and 30 days after the surgeries. Results: In the treated group the VtA remained constant until the 21st day, while in the control group at the same day the value was 0,272 times lower compared to the 3 days period, due to the higher osteoclastic activity. However, at 14 days the VtO was 0,337 times lower in the treated group compared to the control due to the partial inhibition of the transmigration of inflammatory cells responsible for the degradation of the clot and angiogenesis, causing a delay in the formation of granulation/connective tissues, differentiation of osteoblastic cells and in bone tissue formation/remodeling, and consequently in the alveolar bone repair. The immunostaining for COX-2 was observed in various cell types, such as fibroblasts, endothelial cells, inflammatory cells, osteoblasts and osteocytes. The Nm for COX-2 showed no statistical differences between groups from the 3rd to the 21st day, while the WB protein expression was on average 0,232 times lower in the treated group compared to the control. On the other hand, the immunostaining for Runx-2 was more expressive in osteoblasts and osteocytes and rarely in fibroblasts. The Nm for Runx-2 on the treated group was, during the whole experimental period, on average 0,256 times smaller than in the control. The same difference was observed in the protein expression by WB. Conclusion: Within the limits of the present study, its was concluded that the application of Meloxicam daily for 7 days causes a temporary delay in the alveolar bone repair decreasing the expression of proteins COX-2 and RUNX-2.
Foote, Kirsty K. "Characterisation of cardiac function and RUNX expression in two separate models of heart disease." Thesis, University of Glasgow, 2012. http://theses.gla.ac.uk/3746/.
Full textHarrington, Kimberly Stacy. "Intranuclear Trafficking of RUNX/AML/CBFA/PEBP2 Transcription Factors in Living Cells: A Dissertation." eScholarship@UMMS, 2003. https://escholarship.umassmed.edu/gsbs_diss/104.
Full textRibeiro, Larissa Nogueira Soares. "Avaliação da remodelação óssea em alvéolos dentários, após a aplicação do laser de baixa potência." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/58/58135/tde-13122013-120105/.
Full textIntroduction: Low Power Laser (LPL) therapy has been used in Dentistry to achieve many objectives, particularly to decrease the healing period on wound healing. Objectives: The purpose of this in vivo study was to evaluate qualitatively and quantitatively the irradiation effect with LPL in the bone remodeling process after tooth extraction in young rats. Material and Method: 60 Wistar rats were used, randomly distributed in the following groups: Control Group (n=30) animals with tooth extraction without LPL application, and Experimental Group (n=30) animals with tooth extraction (Ext) and LPL application (Ext+LPL) on the three fist days (54 J/cm2 per day). The animals were euthanized after the end periods of 1, 2, 3, 5, 7 and 10 days after the tooth extraction. This study looked into the effects of laser application on the alveolar repair through light microscopy, polarized light and immunostaining. After that, the following parameters were evaluated: 1) percentage of bone formation inside the dental alveolus; 2) degree of inflammatory process; 3) degree of collagen maturation; 4) immunostaining for TRAP and RUNX-2. All the results obtained were submitted to statistical analysis over the ANOVA and Mann- Whitney (p<0.05) test. Results: It was observed that the alveolus from the Experimental Group presented an improved repair process when compared to the Control Group, characterized by faster blood clot organization, more apparent fibroblasts proliferation on the remnants of the periodontal ligament, earlier and more intense collagen organization and bone generation. Conclusion: The use of Low Power Laser accelerated the bone generation process during the experimental initial period, although there was no difference in the ossification process in the final periods.
小川, 伸哉. "転写因子 STAT5 と Runx の結合と相互抑制." 京都大学 (Kyoto University), 2008. http://hdl.handle.net/2433/123793.
Full textJenkins, Catherine Elfi Sarah. "Mechanisms of acute leukemia disease initiation and maintenance through manipulation of IGF1R and RUNX family members." Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/61331.
Full textMedicine, Faculty of
Graduate
Poulsen, Rachel Lynn. "XPRIME: A Method Incorporating Expert Prior Information into Motif Exploration." BYU ScholarsArchive, 2009. https://scholarsarchive.byu.edu/etd/2083.
Full textBras, Stéphanie. "É́tude des fonctions normales et pathologiques des facteurs de transcription de type RUNX au cours de l'hématopoïèse chez Drosophila melanogaster." Toulouse 3, 2012. http://thesesups.ups-tlse.fr/1714/.
Full textHematopoiesis is the physiological process allowing the production of blood cells. This process is finely tuned by many transcription factors and in particular by members of the GATA and RUNX families that play essential roles, from the emergence of hematopoietic stem cells up to their differentiation into multiple lineages. In humans, many genetic alterations affecting RUNX1 are associated with blood cell diseases. In particular, the translocation t(8; 21), which causes the expression of the fusion protein RUNX1-ETO (RE), is associated with ± 12% of cases of acute myeloid leukemia (AML). During my PhD, I studied how RUNX factors regulate both normal and leukemic blood cell development. For this, I took advantage of the conservation of the gene networks regulating hematopoiesis from vertebrates to Drosophila. In particular, Lozenge (LZ) and Serpent (SRP), the respective homologues of RUNX1 and GATA1 also control blood cell development in this insect. To decipher how the activity of RUNX and GATA factors is regulated, we performed an in cellulo RNAi screen to identify regulators of SRP/LZ. Among the candidates from this screen, I focused on MLF (Myeloid Leukemia Factor), whose counterpart in humans (hMLF1) was identified as the target of the t(3; 5) associated with LAM. The results we obtained show that MLF control homeostasis of drosophila hematopoietic system. Thus, MLF is involved in a positive feedback loop controlling LZ activity during hematopoiesis. Moreover, I made a genetic screen in vivo in Drosophila to identify new regulators of the human oncogenic protein RE, which induce leukemic like phénotypes in Drosophila. By screening for genes whose loss of function, induced by RNAi specifically in LZ+ cells, I recovered 26 candidate genes required for RE activity in Drosophila. In fact, I could show that inhibition of one of these genes, Pontin52, reduced the clonogenicity of human leukemic cells expressing RE. Finally, my results show that MLF is required for RE-induced preleukemic phenotypes in Drosophila. These results suggest that the control of RUNX factors stability by MLF may play an important role in hematopoiesis and leukemogenesis
Osman, Dani. "Drosophila hematopoietic cells as a model to study in vivo the activity of the human oncogene AML1-ETO." Toulouse 3, 2009. http://thesesups.ups-tlse.fr/1287/.
Full textHematopoiesis is a complex and dynamic process that leads to the formation and continuous blood cells replenishment. During this process, a series of transcription factors controls the appearance, commitment and differentiation of stem cells into specific lineages. In particular, in vertebrates, the factor RUNX1/AML1 (for Acute Myeloid Leukemia 1) is required for the emergence of hematopoietic stem cells and for the differentiation of both myeloid and lymphoid lineages. In humans, several genetic alterations affecting AML1 are linked to the development of different hemopathies. Notably, the chromosomal translocation t (8; 21), encoding the fusion protein AML1-ETO, is associated with 10-15% of cases of acute myeloid leukemia. It has been described that AML1-ETO acts essentially by interfering with AML1 function during hematopoietic differentiation. However, the mode of action of this oncogene remains poorly understood and few factors modulating its activity are known. Recently, numerous studies have shown that several aspects of hematopoietic development are conserved from Drosophila to vertebrates. Notably, transcription factors of RUNX and GATA families, which are key players in vertebrate's hematopoiesis, also control the development of blood cells in Drosophila. Taking advantage of the phylogenetic conservation of genetic circuitry regulating hematopoiesis between humans and Drosophila and of the powerful genetic tools available in Drosophila, we assessed whether Drosophila can provide a suitable model system to study the mechanism of action of the human oncogene AML1-ETO and to identify modulators of its activity. Our results show that AML1-ETO exerts in Drosophila blood cells expressing a RUNX factor (Lozenge, LZ) similar effects to those observed in human leukemic cells carrying the t(8;21), namely a differentiation blockage and an increased proliferation. In addition, by performing a large scale in vivo screen based on RNA interference, we identified calpainB as required for AML1-ETO-induced blood cell disorders in Drosophila. In addition, we showed that calpains inhibition resulted in AML1-ETO degradation and reduced the clonogenic potential of human leukemic cells expressing intrinsically this chimera. Our results establish that Drosophila can be used as an alternative model to better understand AML1-ETO function and to identify new factors regulating its activity
Bataille, Laetitia. "Mécanismes de régulation de l'hématopoïèse embryonnaire chez la Drosophile." Toulouse 3, 2006. http://www.theses.fr/2006TOU30050.
Full textHaematopoietic development give rise to different specialised blood cell types. In Drosophila embryo, the blood cell progenitors (prohemocytes) give rise to two differentiated cell types : plasmatocytes and crystal cells. We have investigated the mechanism of regulation of this process in the fruit fly. We have shown that serpent encodes different isoforms and that the activity of the GATA transcription factor Serpent is modulated by different cofactors, U-Shaped (FOG) or Lozenge (RUNX), during haematopoiesis. Secondly, we have undertaken an in vivo analysis of the mechanism of segregation of the two embryonic blood cell lineages. We find that prohemocytes are bipotent progenitors, which the fate is determined by a dynamic interplay between the lineage-specific transcription factors, Gcm/Gcm2 and Lz. The resolution of the choice of blood cell fate correspond to an original two-steps process in which Gcm/Gcm2 control the initiation and next the maintenance of the crystal cell fate
Yamamoto, Hiromitsu. "Runx2 and Runx3 are essential for chondrocyte maturation and Runx2 regulates limb growth through induction of Indian hedgehog." Kyoto University, 2004. http://hdl.handle.net/2433/145283.
Full textHutka, Scott Alan. "Leatherfolk On The Run: Leatherfolk, Leather Runs, Identity and Place." Kent State University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=kent1290971538.
Full textFitzgerald, Mark. "Evidence For The Involvement Of Runx1 And Runx2 In Maintenance Of The Breast Cancer Stem Cell Phenotype." ScholarWorks @ UVM, 2018. https://scholarworks.uvm.edu/graddis/888.
Full textKundrotaitė, Julija. "Baltiškieji elementai Kalevaloje." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2005. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2005~D_20050613_115457-36769.
Full textTaber, Thomas Howland. "Thyroid Hormone Receptor SS (trß) Regulation Of Runt-Related Transcription Factor 2 (runx2) In Thyroid Tumorigenesis: Determination Of The Trß Nuclear Protein Complexes That Associate With The Runx2 Gene." ScholarWorks @ UVM, 2017. http://scholarworks.uvm.edu/graddis/820.
Full textCatanese, Carly. "EFFECT OF EXTERNAL COUNTERPULSATION (ECP) ON DELAYED ONSET MUSCLE SORENESS (DOMS) IN LONG DISTANCE RUNNERS." Cleveland State University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=csu1199458812.
Full textVaughan, Tanya, and n/a. "Identifying Genes Influencing Bone Mineral Density." Griffith University. School of Health Science, 2004. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20040430.161453.
Full textVaughan, Tanya. "Identifying Genes Influencing Bone Mineral Density." Thesis, Griffith University, 2004. http://hdl.handle.net/10072/366470.
Full textThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Health Sciences
Full Text
Ingólfsson, Ármann. "Run by run process control." Thesis, Massachusetts Institute of Technology, 1991. http://hdl.handle.net/1721.1/13036.
Full textIncludes bibliographical references (leaves 115-118).
by Ármann Ingólfsson.
M.S.
Linnér, Alexander. "Disposition runt global uppvärmning. : Karlstads studenters disposition runt den globala uppvärmningen." Thesis, Karlstads universitet, Institutionen för geografi, medier och kommunikation (from 2013), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-77186.
Full textFahr, Mignon. "As Runs the Deer." ScholarWorks@UNO, 2003. http://scholarworks.uno.edu/td/11.
Full textBreitholtz, Adelina. "Social organisering runt naturresurser." Thesis, Uppsala universitet, Institutionen för arkeologi och antik historia, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-324497.
Full textMaccolini, Serena. "Ricerca di una risonanza ad alta massa nello stato finale mu+mu- a sqrt(s)=13 TeV con il rivelatore CMS." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/9415/.
Full textMoyne, William P. (William Patrick). "Run by run control : interfaces, implementation, and integration." Thesis, Massachusetts Institute of Technology, 1995. http://hdl.handle.net/1721.1/38048.
Full textWilliams, Henrik. "Åsrunan användning och ljudvärde i runsvenska steninskrifter /." Uppsala, Sweden : Institutionen för nordiska språk, Uppsala universitet, 1990. http://books.google.com/books?id=_zlcAAAAMAAJ.
Full textDuque-Estrada, Elliott Bryce. "Hell Run." Digital Commons at Loyola Marymount University and Loyola Law School, 2016. https://digitalcommons.lmu.edu/etd/286.
Full textGrant, Michael E. (Michael Edward). "China Run." Thesis, North Texas State University, 1986. https://digital.library.unt.edu/ark:/67531/metadc500963/.
Full textCrowder, Wade (Wade Allen). "Jonica Run." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc500879/.
Full textJakobczyk, Hélène. "Rôles de RUNX1 dan la pathogenèse des leucémies aiguës lymphoblastiques à réarrangement ETV6-RUNX1." Thesis, Rennes 1, 2018. http://www.theses.fr/2018REN1B033.
Full textB-cell precursor acute lymphoblastic leukemia (B-ALL) is the most common pediatric cancer. In this type of leukemia, one of the most common genetic abnormalities is the ETV6-RUNX1 rearrangement. This malignancy is described as a two "hits" model. The first event occurs mainly in utero and generates the fusion gene ETV6-RUNX1. The second event consists in the acquisition of additional genetic abnormalities after birth. These aberrant genomic modifications have been described as resulting from abnormal activity of the RAG recombinase. Our work consisted initially in completing the leukemogenesis model. In continuing our study of ETV6-RUNX1 B-ALL, we focused on the role of RUNX1, an upregulated gene in this type of leukemia. All results confirm the predominant role of RUNX1 in hematopoiesis and leukemogenesis thanks to its ability to associate with proteins with different functions and its involvement in the transcription of key genes in hematology. Our results therefore open new perspectives in understanding the control of transcriptional activity of RUNX1 and its role in malignant hematology
Duran, Villalobos Carlos Alberto. "Run-to-run modelling and control of batch processes." Thesis, University of Manchester, 2016. https://www.research.manchester.ac.uk/portal/en/theses/runtorun-modelling-and-control-of-batch-processes(1d42c508-b96d-4ee6-96ad-ec649a199913).html.
Full textAxén, Albin, and Max Karlberg. "Norden runt, ett pedagogiskt spel." Thesis, Södertörn University College, Lärarutbildningen, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-1257.
Full textUppsatsen handlar om pedagogiska spel i skolundervisningen. Författarna har konstruerat och provspelat ett pedagogiskt spel kallat ”Norden runt” i två skolor i Stockholmsområdet. I uppsatsen redovisas resultaten av undersökningen samt förs en diskussion kring pedagogiska spel och hur de kan användas i skolan.
The subject of this thesis is the pedagogic game in education. The researchers have created and developed a pedagogic game called “Nordic tour”, and tested the game in two schools in
Haninge and Sollentuna. The result of the test, the game and a discussion of the pedagogic game and how they can be used in education is presented in this thesis.
Zhu, Yurong. "Large Deviations on Longest Runs." Thesis, Linköpings universitet, Matematisk statistik, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-128822.
Full textPettersson, Nils. "Samverkan runt planeringsverktyg för hållbarhet." Thesis, Stockholms universitet, Kulturgeografiska institutionen, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-156885.
Full textHallgren, Erika. "Uppföljande lavinventering runt Domsjö Fabriker." Thesis, Umeå universitet, Institutionen för ekologi, miljö och geovetenskap, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-176959.
Full textCampbell, William Jarrett. "Model predictive run-to-run control of chemical mechanical planarization /." Digital version accessible at:, 1999. http://wwwlib.umi.com/cr/utexas/main.
Full textBäckström, Stefan. "The hematopoietic transcription factor RUNX1 : a structural view." Doctoral thesis, Umeå University, Umeå Centre for Molecular Pathogenesis (UCMP), 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-192.
Full textThe malfunction of the transcriptional regulator RUNX1 is the major cause of several variants of acute human leukemias and its normal function is to regulate the development of the blood system in concert with other transcriptional co-regulators. RUNX1 belongs to a conserved family of heterodimeric transcription factors that share a conserved DNA binding domain, the Runt domain (RD), named after the first member of this group – Runt - found in Drosophila melanogaster. The binding partner CBFβ serves as a regulator of RUNX by enhancing its DNA binding affinity through an allosteric mechanism.
The main focus ofo my thesis work has been the crystallization and structural analysis of the RUNX1 RD and involved also more technical methodological aspects that can be applied to X-ray crystallography in general.
The high resolution crystal structure of the free RD shows that this immunoglobulin-like molecule undergoes significant structural changes upon binding to both CBFβ and DNA. This involves a large flip of the L11 loop from a closed conformation in the free protein to an open conformation when CBFβ and/or DNA are bound. We refer to this transition as the “S-switch”. Smaller but significant conformational changes in other parts of the RD accompany the “S-switch”. We suggest that CBFβ triggers and stabilizes the “S-switch” which leads to the conversion of the RD into a conformation enhanced for DNA binding.
During the structural analysis of the RD we identified two chloride ions that are coordinated by residues otherwise involved in DNA binding. In electrophoretic mobility-shift analyses (EMSA) we demonstrated a chloride ion concentration dependent stimulation of the DNA binding affinity of RUNX1. We further showed by NMR line width broadening experiments that the chloride binding occurred within the physiological range. A comparable DNA binding stimulation of RUNX1 was seen in the presence of negative amino acids. This suggests a regulation of the DNA binding activity of RUNX1 proteins through acidic amino acid residues possibly provided by activation domains of transcriptional co-regulators that interact with RUNX1.
The use of the anomalous signal from halide ions has become a powerful technique for obtaining phase information. By replacing the sodium chloride with potassium bromide in the crystallisation conditions of the RD, we could demonstrate in a single wavelength anomalous diffraction (SAD) experiment that the anomalous signal from 2 bromide ions were sufficient to phase a 16 kDa protein. Due to lack of completeness in the low-resolution shells caused by overloaded intensities, density modification schemes failed and the resulting electron density maps were not interpretable. By combining the highresolution
synchrotron data with low-resolution data from a native data set collected on a home X-ray source, the density modified bromide phases gave easily traceable maps.
Hyde, Spencer. ""Let It Run"." Thesis, University of North Texas, 2018. https://digital.library.unt.edu/ark:/67531/metadc1248507/.
Full textDeZeeuw, Zane Truman. "Run Me Dusk." TopSCHOLAR®, 2018. https://digitalcommons.wku.edu/theses/3043.
Full text