Dissertations / Theses on the topic 'Runt related transcription factor'
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Mümmler, Carlo [Verfasser], and Melanie [Akademischer Betreuer] Königshoff. "Runt-related transcription factor 2 in pulmonary fibrosis / Carlo Mümmler ; Betreuer: Melanie Königshoff." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2019. http://d-nb.info/1180285786/34.
Full textCuringa, Gabrielle Mercedes. "The role of runt-related transcription factor 2 in arterial smooth muscle cell mineralization /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/6353.
Full textIsehaq, Saif Said Al-Huseini. "Deletion of IκB-Kinase β in Smooth Muscle Cells Induces Vascular Calcification Through β-Catenin-Runt-Related Transcription Factor 2 Signaling." Kyoto University, 2018. http://hdl.handle.net/2433/232306.
Full textAlsabeelah, Nimer Fehaid N. "Vascular calcification in rat cultured smooth muscle cells : a role for nitric oxide." Thesis, University of Hertfordshire, 2016. http://hdl.handle.net/2299/17217.
Full textVaughan, Tanya, and n/a. "Identifying Genes Influencing Bone Mineral Density." Griffith University. School of Health Science, 2004. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20040430.161453.
Full textVaughan, Tanya. "Identifying Genes Influencing Bone Mineral Density." Thesis, Griffith University, 2004. http://hdl.handle.net/10072/366470.
Full textThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Health Sciences
Full Text
Taber, Thomas Howland. "Thyroid Hormone Receptor SS (trß) Regulation Of Runt-Related Transcription Factor 2 (runx2) In Thyroid Tumorigenesis: Determination Of The Trß Nuclear Protein Complexes That Associate With The Runx2 Gene." ScholarWorks @ UVM, 2017. http://scholarworks.uvm.edu/graddis/820.
Full textCardoso, Camila Lopes. "Análise morfométrica e molecular da alveolite induzida em ratos com diferentes modalidades de tratamento." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/25/25132/tde-27052009-102252/.
Full textDry socket is an inflammatory postoperative complication that undertakes sockets of recently extracted teeth. The incidence of such complication varies from 1 to 4% and might reach up to 30%. The objective of this study was to analyze the biological mechanisms involved in the repair process of intentionally infected sockets in mice; compare different treatment conditions and correlate the results of two different analysis (microscopic and molecular). 84 mice were used in this study, divided according the following groups: I: uninfected socket; II: infected socket without any treatment; III: infected socket treated with irrigation of 2% sodium iodide and 3% hydrogen peroxide solution at 1:1 proportion; and IV: infected socket submitted to curettage, physiological saline solution irrigation and fulfillment with metronidazole base paste. The animals were killed at a postoperative period of 6, 15 and 28 days. A quantitative analysis was performed using a RealTimePCR to evaluate the genes expression involved [Collagen Type I (COL-I), vascular endothelial growth factor (VEGF), osteocalcin (OCN), alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2) and tumor necrosis factor-alpha (TNF-\'alpha\')], in the repair process, correlating its expression with the microscopic characteristics observed in both qualitative and quantitative manner. Based in the results of the microscopic and molecular analysis, it can be concluded that the RUNX2, OCN and TNF-\'alpha\' markers can be used as indicators to evaluate the dry socket bone neoformation and inflammatory infiltrate quantity. The ALP and VEGF markers did not represented appropriately what was observed microscopically. Although the dry socket treatment with metronidazole base paste promotes an increase in the bone neoformation density at 28 days, no difference was found among the treatments.
Bäckström, Stefan. "The hematopoietic transcription factor RUNX1 : a structural view." Doctoral thesis, Umeå University, Umeå Centre for Molecular Pathogenesis (UCMP), 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-192.
Full textThe malfunction of the transcriptional regulator RUNX1 is the major cause of several variants of acute human leukemias and its normal function is to regulate the development of the blood system in concert with other transcriptional co-regulators. RUNX1 belongs to a conserved family of heterodimeric transcription factors that share a conserved DNA binding domain, the Runt domain (RD), named after the first member of this group – Runt - found in Drosophila melanogaster. The binding partner CBFβ serves as a regulator of RUNX by enhancing its DNA binding affinity through an allosteric mechanism.
The main focus ofo my thesis work has been the crystallization and structural analysis of the RUNX1 RD and involved also more technical methodological aspects that can be applied to X-ray crystallography in general.
The high resolution crystal structure of the free RD shows that this immunoglobulin-like molecule undergoes significant structural changes upon binding to both CBFβ and DNA. This involves a large flip of the L11 loop from a closed conformation in the free protein to an open conformation when CBFβ and/or DNA are bound. We refer to this transition as the “S-switch”. Smaller but significant conformational changes in other parts of the RD accompany the “S-switch”. We suggest that CBFβ triggers and stabilizes the “S-switch” which leads to the conversion of the RD into a conformation enhanced for DNA binding.
During the structural analysis of the RD we identified two chloride ions that are coordinated by residues otherwise involved in DNA binding. In electrophoretic mobility-shift analyses (EMSA) we demonstrated a chloride ion concentration dependent stimulation of the DNA binding affinity of RUNX1. We further showed by NMR line width broadening experiments that the chloride binding occurred within the physiological range. A comparable DNA binding stimulation of RUNX1 was seen in the presence of negative amino acids. This suggests a regulation of the DNA binding activity of RUNX1 proteins through acidic amino acid residues possibly provided by activation domains of transcriptional co-regulators that interact with RUNX1.
The use of the anomalous signal from halide ions has become a powerful technique for obtaining phase information. By replacing the sodium chloride with potassium bromide in the crystallisation conditions of the RD, we could demonstrate in a single wavelength anomalous diffraction (SAD) experiment that the anomalous signal from 2 bromide ions were sufficient to phase a 16 kDa protein. Due to lack of completeness in the low-resolution shells caused by overloaded intensities, density modification schemes failed and the resulting electron density maps were not interpretable. By combining the highresolution
synchrotron data with low-resolution data from a native data set collected on a home X-ray source, the density modified bromide phases gave easily traceable maps.
Yu, Wai Man C. Y. O. L. "Targeting the Myocardin-related transcription factor/serum response factor (MRTF/SRF) pathway in conjunctival fibrosis." Thesis, University College London (University of London), 2017. http://discovery.ucl.ac.uk/1559574/.
Full textMcDade, Donna Marie. "Identification of novel target genes for the plasticity-related transcription factor Zif268." Thesis, University of Glasgow, 2007. http://theses.gla.ac.uk/28/.
Full textMontel, Lorraine. "Rôle de Myocardin-Related Transcription Factor dans la mécanotransduction de la cellule musculaire." Sorbonne Paris Cité, 2015. http://www.theses.fr/2015USPCC138.
Full textOur muscles show the most intuitive example of mechanotransduction : when trained they grow whereas immobilized they atrophy. To a mechanical signal, the cells give a biological response. The Sotiropoulos group showed that Serum Response Factor (SRF) and Myocardin-Related Transcription Factor (MRTF-A) play a central role in this phenomenon by regulating cytoskeletal genes and muscle development. The localization of MRTF-A in the cell is regulated by the state of the actin cytoskeleton : in the presence of monomeric actin, MRTF-A in confined to the cytoplasm and SRF inactive. Whereas ir case of monomeric actin scarcity, MRTF-A is accumulated in the nucleus, where it triggers SRF. Using magnetic tweezers and stretching experiments, we demonstrated that, when submitted tc mechanical cues, the actin cytoskeleton reorganizes and MRTF-A is relocated. In response to moderate cues, actin polymerizes and MRTF-A is transported to the nucleus. On the contrary, when the stress is too high, the cytoskeleton is damaged and MRTF-A is confined to the cytoplasm. Using a fluorescent MRTF-A and a panel of actin visualization techniques, we were able to observe the chronology of these events. Moreover, we showed that all the actin visualization techniques interfere with the actin cytoskeleton at a level detectable through MRTF-A localization. Finally we studied the changes in rheological properties of cells submitted to repeated steps of force
Langer, Björn. "Phenotype-related regulatory element and transcription factor identification via phylogeny-aware discriminative sequence motif scoring." Doctoral thesis, Center for Systems Biology Dresden, 2017. https://tud.qucosa.de/id/qucosa%3A31172.
Full textDoecke, James. "Genetic variation in Runx2 related to bone mineral density." Thesis, Griffith University, 2006. http://hdl.handle.net/10072/367841.
Full textThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Medical Science
Griffith Health
Full Text
Hartmann, Rico Martin [Verfasser]. "Functional analysis of arbuscular mycorrhiza-related GRAS transcription factor genes of Medicago truncatula / Rico Martin Hartmann." Hannover : Gottfried Wilhelm Leibniz Universität Hannover, 2018. http://d-nb.info/1165315688/34.
Full textPatel, Jigisha. "Age-related changes in TNF receptors and transcription factor translocation in response to TNFalpha and abeta /." Available to subscribers only, 2007. http://proquest.umi.com/pqdweb?did=1456284711&sid=13&Fmt=2&clientId=1509&RQT=309&VName=PQD.
Full text"Department of Molecular Biology, Microbiology and Biochemistry." Includes bibliographical references (leaves 86-96). Also available online.
Lee, Ching-Jung. "Sox related genes in cerebellar tumours and developing cerebellum : including the identification of a new family of sox-related transcription factor." Thesis, University of Nottingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250589.
Full textCreed, Mitchell Peterson. "Characterization of myocardin related transcription factor A expression and function in systemic scleroderma and collagen gene regulation." Thesis, Boston University, 2013. https://hdl.handle.net/2144/21141.
Full textSystemic sclerosis (SSc) is a clinically heterogenous chronic fibrotic disease which affects skin and internal organs. While the pathogenesis of SSc remains unknown, the hallmark of both localized and diffuse SSc in the skin is the replacement of normal dermal architecture with excessive deposition of collagen and other connective tissue macromolecules. Progressive replacement of tissue architecture by collagen-rich extracellular matrix (ECM) results in functional impairment of affected organs. Fibrotic damage to these affected organs accounts for much of the morbidity and mortality concomitant with SSc, particularly in the lungs. Myofibroblasts are the primary ECM-secreting cells during wound healing and fibrosis. Myocardin-related transcription factor A (MRTF-A), is an important regulator of myofibroblast differentiation, depending on serum response factor (SRF) for smooth muscle actin (SMA) and Sp1 in the regulation of collagen gene expression. MRTF-A continually shuttles between the nucleus and cytoplasm in unstimulated cells. Signals of stress, mechanical force, and migration control MRTF-A movement by a mechanism in which Rho-activated cytoskeletal actin polymerization induces its relocation from the cytoplasm to the nucleus. The major hypothesis in this thesis is that MRTF-A is dysregulated (impairment of a physiological regulatory mechanisms) and/or activated in SSc patients in part through transforming growth factor beta (TGF-β). To test this hypothesis, immunohistochemistry using MRTF-A antibodies was performed on SSc patient skin lesions and healthy control skin. Staining was observed in the epidermis, epidermal structures, vasculature and dermis of SSc and healthy control skin. In the epidermal layer of patients with SSc, there was significantly more nuclear localization of MRTF-A then in normal controls. Prominent staining is also present in endothelial, perivascular and some perivascular inflammatory cells of SSc patients. Perivascular staining was not seen in healthy controls. Interestingly, there was some accumulation of nuclear MRTF-A in areas typical of myofibroblasts in SSc skin, but this staining is not as striking as vascular staining. TGF-β activates MRTF-A in a cell-specific manner. As SSc typically begins within the skin, human dermal fibroblasts (HDF) were grown in culture. HDFs synthesize and secrete collagen to a greater extent when compared to human lung fibroblasts (IMR90 cells). Treatment with TGF-β enhances cytoplasmic localization of MRTF-A at 4-8 hours in HDFs and prolongs nuclear localization. Transgenic mouse lung cells were isolated from an MRTF-A loss-of-function mouse carrying the 3.6 kb proximal promoter of the rat COL1A1 gene driving topaz green fluorescent protein (GFP) (pOB3.6COLGFPtpz). Since angiotensin II (ANG II) may enhance TGF-β response or collagen transcription directly, wild type (WT) and MRTF-A knockout (KO) cells were treated with ANG II and TGF-β. Quantification of collagen transcription by GFP fluorescence and protein synthesis by Western and secretion by Sircol analysis revealed collagen gene expression is consistently lower in KO fibroblasts compared to WT. Total percentage of fluorescent KO cells were consistently lower in comparison to WT cells as well. KO cells do not respond to TGF-β or ANG II treatment, whereas TGF-β increased collagen gene expression by WT cells, but not KO cells. Furthermore, treatment with ANG II did not up-regulate transcription in WT mouse lung fibroblasts. However, TGF-β receptor kinase 1 (TβR-1) inhibitor SB431542 attenuated collagen transcription in both WT and KO fibroblasts regardless of treatment suggesting that the receptor is active with or without MRTF-A possibly with an endogenous ligand produced by these cells. The activation of MRTF-A is an important protein regulating collagen synthesis and may potentially serve as a therapeutic target in future treatments of fibrotic disease such as SSc.
Hariton, Florence A. G. "Anti-stress gene response in cell and tissue ageing : role of transcription factor NF-E2-related factor-2 and effect of dietary activators." Thesis, University of Warwick, 2014. http://wrap.warwick.ac.uk/66553/.
Full textKajita, Yoichiro. "The transcription factor Sp3 regulates the expression of a metastasis-related marker of sarcoma, actin filament-associated protein 1-like 1(AFAP1L1)." Kyoto University, 2013. http://hdl.handle.net/2433/179345.
Full textBolt, Sylvia [Verfasser]. "Functional and molecular characterization of the phylogenetically related ERF102 to ERF105 transcription factor genes in Arabidopsis thaliana / Sylvia Bolt." Berlin : Freie Universität Berlin, 2017. http://d-nb.info/1149050543/34.
Full textMarchal-Duval, Emmeline. "Identification de PRRX1 (Paired Related Homeobox Protein-1), un nouveau facteur de transcription impliqué dans la Fibrose Pulmonaire Idiopathique." Thesis, Sorbonne Paris Cité, 2018. http://www.theses.fr/2018USPCC326.
Full textIdiopathic Pulmonary Fibrosis (IPF) is a chronic pulmonary devastating disease, with no current therapeutic available. Fibroblasts are key cells driving fibrogenesis during IPF. From mesenchymal origin, their phenotype regulation is still poorly understood. And among the potential regulators, only few transcription factors (TF) are specific to these cells. Our work demonstrates for the first time, the implication of the mesenchymal TF PRRX1 in the regulation of fibroblast phenotype during IPF development. In vitro, PRRX1 induce proliferation, survival and myofibroblast differentiation. In vivo, the PRRX1 inhibition by antisens strategy lead to attenuate fibrotic lesions and extracellular matrix deposition of collagen, fibronectine, and smooth muscle actin of bleomycin lung mice. Here we identify PRRX1 as a new fibroblast transcription factor regulator during IPF, and inhibit it could be a promising therapeutic
Adolfi, Mateus Contar. "Desenvolvimento gonadal e clonagem molecular de Dmrt1 (doublesex and mab-3-related transcription factor-1) e Sox9 em teleósteos sul-americanos." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-19032012-162629/.
Full textThe sequences of dmrt1 and sox9 genes were analyzed in pirarucu (Arapaima gigas), lambari (Astyanax altiparanae) and piapara (Leporinus elongatus) using molecular cloning techniques. Fragments of dmrt1 gene were cloned in these three species, and sox9 gene fragments have been acquired only for lambari and piapara. Phylogenetic analysis of the genes was shown to be consistent with the morphological phylogeny of these species. The analysis of the gonadal development in lambari was followed by light microscopy, where it was found the presence of germ cells in the region of the future gonad in 5 days after hatching (dah), and that the formation of ovaries occurs first comparing to the formation of testis. The gene expression of dmrt1 and sox9 by qRT-PCR method show specificity for testis comparing to ovary, which indicates that these genes also have a male-specific role in the adult. In lambari, the genes were expressed in all larval periods, with a decrease of expression levels in 12 dah, and increasing its expression at stage of 33 dah.
Sköld, Mattias. "On VEGF and related factors in neurotrauma /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-986-2/.
Full textXu, Ling. "Transcription Factor 7-like 2 (TCF7L2) Gene Polymorphisms in Relation to the Risk of Type 2 Diabetes in three ethnicities." FIU Digital Commons, 2018. https://digitalcommons.fiu.edu/etd/3815.
Full textPoulain, Marine. "Développement de la lignée germinale femelle humaine." Thesis, Paris 11, 2014. http://www.theses.fr/2014PA11T056/document.
Full textWoman fertility is partially dictated by the set up of the human female germ line. During the last ten years, which saw an increased number of couples consulting for assisted reproductive cares, the hypothesis of an early alteration in reproduction functions has emerged.In the fetal ovary, germ cells enter the path of oogenesis differentiation characterized by meiotic initiation. On this subject, vast majority of the scientific data are obtained from the mouse model, even if differences with human ovarian physiology are widely acknowledged. Therefore it is necessary to extend our knowledge on human ovarian development and identify its perturbations. The objective of my work was to assess a new model to study ovarian growth, studying regulation of meiotic entry and perturbation of germ line differentiation.We sat up a new xenograft model of early human fetal ovaries, when very early meiotic germ cells appear. Organ growth and germ cells differentiation were comparable with in vivo observations. Using this model with an RNA-interference strategy, we inhibited the expression of an oogonia germ cell gene, DMRTA2. This inhibition conducted to a significantly reduced number of germ cells gene that initiated meiosis and DMRTA2 seemed to be required for mitotic-meiotic transition. In another hand, we identified, in the ovary, the expression of germ cells markers described as specifically male in rodent (PLZF, DNMT3L, FGF9, NANOS2 ou CYP26B1). The expression of these markers in the human ovary could explain the observation of mitotic germ cells in late fetal ovaries (30 wpf).In parallel, we tested germ cells sensibility to a synthetic glucocorticoid, dexamethasone, administrated during pregnancy in some justified pathologies. We observed an increased expression of PLZF that could explain the decreased number of germ cells observed in treated ovaries.In conclusion, we identified a new gene expressed in human fetal ovaries, potentially involved in the meiotic entry, and we extended our knowledge to characterized human germ line development. However, many points have to be clarified, as the possible competence of late mitotic germ cells to form oocytes
Langer, Björn [Verfasser], Michael [Akademischer Betreuer] Hiller, Ivo [Gutachter] Sbalzarini, and Peter [Gutachter] Stadler. "Phenotype-related regulatory element and transcription factor identification via phylogeny-aware discriminative sequence motif scoring / Björn Langer ; Gutachter: Ivo Sbalzarini, Peter Stadler ; Betreuer: Michael Hiller." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2018. http://d-nb.info/1226813224/34.
Full textLanger, Björn [Verfasser], Michael [Akademischer Betreuer] Hiller, Ivo Fabian [Gutachter] Sbalzarini, and Peter [Gutachter] Stadler. "Phenotype-related regulatory element and transcription factor identification via phylogeny-aware discriminative sequence motif scoring / Björn Langer ; Gutachter: Ivo Sbalzarini, Peter Stadler ; Betreuer: Michael Hiller." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2018. http://d-nb.info/1226813224/34.
Full textBukka, Prasanna L. "Regulation of parathyroid hormone-related peptide gene expression in osteoblast-like cells : the role of an intronic minisatellite ans Sp1 transcription factor binding sites in the promoter region." Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=19451.
Full textHuang, Liang. "Functions and Regulatory Mechanisms of the Rel Family Transcription Factors, Dorsal and Dif, and the UBC9 Family SUMO Conjugase, Lesswright, in DrosophilaHematopoiesis." Ohio : Ohio University, 2006. http://www.ohiolink.edu/etd/view.cgi?ohiou1162613472.
Full textGeißler, Maximilian Per [Verfasser], and Robert [Akademischer Betreuer] Grosse. "Die Funktion des Linker of Nucleoskeleton and Cytoskeleton (LINC)-Komplexes und nukleärer Aktin-Polymerisation in der Myocardin Related Transcription Factor A (MRTF-A) regulierten Tumorzellinvasion / Maximilian Per Geißler ; Betreuer: Robert Grosse." Marburg : Philipps-Universität Marburg, 2020. http://d-nb.info/122404682X/34.
Full textSilva, Thatiana Evilen da. "Pesquisa de mutações no gene DMRT1 em pacientes portadores de distúrbios do desenvolvimento sexual (DDS) 46,XY por anormalidades gonadais." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-10102012-102725/.
Full textIntroduction Dmrt1 gene is a very important factor in inducing male sex determination, and more recently it has been demonstrated that Dmrt1 plays a significant role in ovary development. DMRT1 deletions have rarely been identified in patients with 46,XY gonadal dysgenesis (GD) without syndromic features. Objective- To screen for the presence of DMRT1 haploinsufficiency (deletions and/or inactivating mutations) in a large cohort of non-syndromic patients with disorder of sex development (DSD) due to abnormalities of gonadal development. DMRT1 polymorphisms, as potential susceptibility factors for gonadal abnormalities, were also investigated. Subjects and Methods- We evaluated 39 patients with 46,XY GD: 24 patients with the partial, and 15 with the complete form. The entire coding region (éxons 2-5) of DMRT1 and the DM domain (exon 1) were PCR-amplified and direct sequenced. Multiplex ligation probe amplification (MLPA) analysis of DMRT1 was carried out using a commercial kit. Results- Partial or total deletion of DMRT1 was not identified by MLPA technique. Eight allelic variants of DMRT1 were identified. The novel variant c.968-15insTTCTCTCT was identified in 6.4% and in 14.3% of the alleles of 46,XY patients and control subjects, respectively Conclusion- This study suggest that complete or partial DMRT1 deletions and inactivating mutations are not frequently found in patients with abnormalities of gonadal development. Additionally, none of the allelic variants identified in this cohort of patients could be considered a potential polymorphic susceptibility marker for gonadal dysgenesis
Hudson, Jaylen Braxton. "Identifying Endogenous Binding Partners of Btf and TRAP150." Wright State University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=wright1591182283366217.
Full textWolf, Horrell Erin M. "Regulation of UV-Protective Pathways Downstream of the Melanocortin 1 Receptor in Melanocytes." UKnowledge, 2016. http://uknowledge.uky.edu/physiology_etds/29.
Full textXu, M., X. Wang, J. Tan, K. Zhang, X. Guan, Laurence H. Patterson, H. Ding, and H. Cui. "A novel Lozenge gene in silkworm, Bombyx mori regulates the melanization response of hemolymph." 2015. http://hdl.handle.net/10454/9422.
Full textRunt-related (RUNX) transcription factors are evolutionarily conserved either in vertebrate or invertebrate. Lozenge (Lz), a members of RUNX family as well as homologue of AML-1, functions as an important transcription factor regulating the hemocytes differentiation. In this paper, we identified and characterized RUNX family especially Lz in silkworm, which is a lepidopteran model insect. The gene expression analysis illustrated that BmLz was highly expressed in hemocytes throughout the whole development period, and reached a peak in glutonous stage. Over-expression of BmLz in silkworm accelerated the melanization process of hemolymph, and led to instantaneously up-regulation of prophenoloxidases (PPOs), which were key enzymes in the melanization process. Further down-regulation of BmLz expression by RNA interference resulted in the significant delay of melanization reaction of hemolymph. These findings suggested that BmLz regulated the melanization process of hemolymph by inducing PPOs expression, and played a critical role in innate immunity defense in silkworm.
Stewart, Trudy Ann. "Expression of runt related transcription factor 2 and vascular endothelial growth factor in the pulp, periodontal ligament and alveolar bone: an immunohistochemical study using a rat ankylotic model." Thesis, 2013. http://hdl.handle.net/2440/82553.
Full textThesis (D.Clin.Dent.) -- University of Adelaide, School of Dentistry, 2013
Giesen, Markus [Verfasser]. "Die Rolle der Transkriptionsfaktoren "runt-related transcription factor-2" (RUNX2) und Osterix in humanen Osteoblasten : In-vitro-Untersuchung zu Differenzierung und Einfluss der Hemmung von RUNX2 mittels siRNA / vorgelegt von Markus Giesen." 2008. http://d-nb.info/989276775/34.
Full textLEE, M. I., and 李美宜. "Recognizing Cancer-related Genes based on Transcription Factor Binding Sites." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/11997758257999628220.
Full text臺中健康暨管理學院
資訊科學與應用學系碩士班
93
Abstract The purpose of transcription factors (TFs) is to regulate the expression of other genes. They are also the key-point to control if mutation will occur on promoter region or not? Current researchers on TFs mainly focus on predicting motifs using algorithms such as Multiple Em for Motif Elicitation (MEME), Genetic Algorithm (GA), and Gibbs Sampler. In this thesis, we propose a new approach to predict possible cancer-related genes based on transcription factor binding sites (TFBS). The experimented TFBS that are binding on promoter region and the known cancer-related genes have been collected from TFSEARCH and CHIP websites, respectively. The TFBS that result in mutation of genes are selected. We then analyze the occurrence frequencies of these TFBS to investigate the relations of TFBS and possible cancer-related genes. We also discuss the two-factor case of analyzing the relations of two TFBS and possible cancer-related genes. Our results show that the TFBS-based approach for predicting possible cancer-related genes is a reliable method to recognize possible cancer-related genes.
HUAI-WEN, CHANG, and 張懷文. "Analysis of Transcription Factor Binding Site in Cancer-related Genes." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/16853433434148383577.
Full text亞洲大學
生物資訊學系碩士班
96
The processes of oncogenesis are multiple steps. And the over-expression of oncogenes plays a very important role in the initial state of numerous cancer cases. Mining the human genome to identify genetic mutations that cause cancer is like looking for needles in a haystack, especially by using the traditional one-by-one analysis method. Hence, it is a very important issue in bioinformatics to find out any information about cancer-related factors from these huge relational databases. The purpose of this research is to analyze existed known cancer-related genes and focus on the investigation of their transcription factor binding sites (TFBS), and predict the possible influence of gene control of tuning normal genes into cancer ones. The method is that we collect the issued TFBS in human genes from TFBS databases over the Internet, and then analyze the occurrence frequencies of these TFBS to investigate the relations between TFBS and the cancer-related genes. Both cases of single TFBS and multiple TFBS are investigated in this thesis. The results of this research can be used to conclude that if higher occurrence frequencies of transcription factors have problem in the process of transcription, they will influence the performance of gene transcription, thus become important possible factors of tuning normal genes into cancer ones. This feature can be considered as a gene marker to identify if a gene is possible to be a cancerous gene, and it will be great helpful for promoting high-precision of searching cancer-related genes on medical.
To, Sarah. "Molecular characterization of the CP2-related transcription factor, CRTR-1." 2009. http://hdl.handle.net/2440/59773.
Full texthttp://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1374290
Thesis (Ph.D.) -- University of Adelaide, School of Molecular and Biomedical Science, 2009
To, Sarah. "Molecular characterization of the CP2-related transcription factor, CRTR-1." Thesis, 2009. http://hdl.handle.net/2440/59773.
Full textThesis (Ph.D.) -- University of Adelaide, School of Molecular and Biomedical Science, 2009
Parker, Benjamin. "Characterization of fracture healing in myocardin-related transcription factor-A deficient mice." Thesis, 2018. https://hdl.handle.net/2144/31277.
Full textLi, Chendi. "Myocardin-related transcription factor A regulates conversion of progenitors to beige adipocytes." Thesis, 2015. https://hdl.handle.net/2144/16110.
Full textChiasson, David Michael. "Characterization of GmSAT1 and related proteins from legume nodules." Thesis, 2012. http://hdl.handle.net/2440/80104.
Full textThesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2012
MURAKAMI, Masashi, and 真史 村上. "ATRA inhibits ceramide kinase transcription through an ATRA-related transcription factor, COUP-TFI, in a human neuroblastoma cell line, SH-SY5Y." Thesis, 2010. http://hdl.handle.net/2237/14433.
Full textWang, Yi Chun, and 王峻逸. "Regulation of the expression of the transcription factor Snail, a protein related to tumor metastasis." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/83671243993161617970.
Full text慈濟大學
分子生物及細胞生物研究所
92
Tumorgenesis is a multi-step process involving genetic alterations that lead to tumor formation and tumor invasion. Tumor metastasis is the most severe stage in the tumorgenesis process. However, the molecular mechanism of metastasis remains unclear. Recent evidence has indicated that the expression of the transcription factor Snail plays an important role in tumor metastasis. Snail is a zinc-finger transcription factor, which represses the expression of E-cadherin and induces epithelial-mesenchymal transition (EMT) associated with tumor progression and an invasive phenotype. Thus, it is important to understand the mechanism how Snail is upregulated in metastatic cancer cells. In the present study, we isolated the regulatory elements of the human Snail gene by polymerase chian reaction (PCR) and inserted these serially deleted regulatory fragments of Snail into a luciferase reporter vector, respectively. In order to select the model cell line, the reverse transcription polymerase chian reaction (RT-PCR) was carried out to examine the cDNA expression level of Snail in various cancer cell lines. In a functional assay, analysis of these constructs in melanoma cell line (A2058) revealed the strongest promoter activity among the regions is located between —262 and —91. This region was further investigated by electrophoresis mobility shift assay (EMSA) and was found to contain some protein complexes in region between —111 and —91. Preliminary database analysis showed it contained putative SP1 binding sites. The co-transfection experiment showed that the SP1 could slightly up regulate the activity of Snail expression. In addition, the EMSA experiments using recombinant SP1 protein revealed that SP1 interacts with the fragment, which is between —111 and —91. Our results suggest that SP1 is functional in regulating the Snail gene
Peng, Rui. "Characterization of the essential pre-mRNA splicing factor PSF investigation of RNA binding specificity and splicing-related complex formation /." Diss., 2005. http://etd.library.vanderbilt.edu/ETD-db/available/etd-08112005-103004/.
Full textRaimondo, Anne. "Identification of downstream target genes and analysis of obesity-related variants of the bHLH/PAS transcription factor single-minded 1." Thesis, 2011. http://hdl.handle.net/2440/73199.
Full textThesis (Ph.D.) -- University of Adelaide, School of Molecular and Biomedical Science, 2011
Krishnaswamy, Sowmya. "Dissecting the role of pathogenesis related-10 (PR-10) proteins in abiotic stress tolerance of plants." Phd thesis, 2011. http://hdl.handle.net/10048/1746.
Full textPlant Science
Bian, Hejiao. "The role of myocardin related transcription factor A in controlling the commitment of progenitors to adipose lineage versus osteoblastic lineage." Thesis, 2015. https://hdl.handle.net/2144/16204.
Full text