Journal articles: 'RSC complex'

1

Mao, Steve. "The architecture of the RSC complex." Science 366, no. 6467 (November 14, 2019): 833.3–833. http://dx.doi.org/10.1126/science.366.6467.833-c.

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Ye, Youpi, Hao Wu, Kangjing Chen, Cedric R. Clapier, Naveen Verma, Wenhao Zhang, Haiteng Deng, Bradley R. Cairns, Ning Gao, and Zhucheng Chen. "Structure of the RSC complex bound to the nucleosome." Science 366, no. 6467 (October 31, 2019): 838–43. http://dx.doi.org/10.1126/science.aay0033.

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The RSC complex remodels chromatin structure and regulates gene transcription. We used cryo–electron microscopy to determine the structure of yeast RSC bound to the nucleosome. RSC is delineated into the adenosine triphosphatase motor, the actin-related protein module, and the substrate recruitment module (SRM). RSC binds the nucleosome mainly through the motor, with the auxiliary subunit Sfh1 engaging the H2A-H2B acidic patch to enable nucleosome ejection. SRM is organized into three substrate-binding lobes poised to bind their respective nucleosomal epitopes. The relative orientations of the SRM and the motor on the nucleosome explain the directionality of DNA translocation and promoter nucleosome repositioning by RSC. Our findings shed light on RSC assembly and functionality, and they provide a framework to understand the mammalian homologs BAF/PBAF and the Sfh1 ortholog INI1/BAF47, which are frequently mutated in cancers.

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Cairns, Bradley R., Yahli Lorch, Yang Li, Mincheng Zhang, Lynne Lacomis, Hediye Erdjument-Bromage, Paul Tempst, Jian Du, Brehon Laurent, and Roger D. Kornberg. "RSC, an Essential, Abundant Chromatin-Remodeling Complex." Cell 87, no. 7 (December 1996): 1249–60. http://dx.doi.org/10.1016/s0092-8674(00)81820-6.

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Romeo, Martin J., Melinda L. Angus-Hill, Andrew K. Sobering, Yoshiaki Kamada, Bradley R. Cairns, and David E. Levin. "HTL1 Encodes a Novel Factor That Interacts with the RSC Chromatin Remodeling Complex in Saccharomyces cerevisiae." Molecular and Cellular Biology 22, no. 23 (December 1, 2002): 8165–74. http://dx.doi.org/10.1128/mcb.22.23.8165-8174.2002.

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ABSTRACT RSC is an essential chromatin remodeling complex in Saccharomyces cerevisiae that performs central roles in transcriptional regulation and cell cycle progression. Here we identify Htl1 as a novel factor that associates with the RSC complex both physically and functionally. We isolated HTL1 through a genetic screen for mutants that displayed additive growth defects with a conditional mutation in the protein kinase C gene (PKC1), which has been suggested through genetic connections to interact functionally with RSC. Several lines of evidence connect HTL1 to RSC function. First, an htl1Δ mutant displayed temperature-sensitive growth and a G2/M cell cycle arrest at restrictive temperatures, a phenotype similar to that of strains with conditional mutations in essential RSC components. Second, we isolated RSC3, which encodes a component of the RSC complex, as a dosage suppressor of the htl1Δ growth arrest. Third, an htl1Δ mutant displayed additive growth defects with conditional rsc3 alleles. Fourth, overexpression of HTL1 suppressed the growth defect of a strain with a conditional mutation in another RSC component, RSC8. Finally, we demonstrate that Htl1 is a nuclear protein that can associate in vivo with a fraction of the RSC complex. We propose that an RSC-Htl1 complex acts coordinately with protein kinase C to regulate the G2/M transition.

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Asturias, F. J., W. H. Chung, R. D. Kornberg, and Y. Lorch. "Structural analysis of the RSC chromatin-remodeling complex." Proceedings of the National Academy of Sciences 99, no. 21 (October 4, 2002): 13477–80. http://dx.doi.org/10.1073/pnas.162504299.

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Hsu, Jing-mei, Jian Huang, Pamela B. Meluh, and Brehon C. Laurent. "The Yeast RSC Chromatin-Remodeling Complex Is Required for Kinetochore Function in Chromosome Segregation." Molecular and Cellular Biology 23, no. 9 (May 1, 2003): 3202–15. http://dx.doi.org/10.1128/mcb.23.9.3202-3215.2003.

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ABSTRACT The accurate segregation of chromosomes requires the kinetochore, a complex protein machine that assembles onto centromeric DNA to mediate attachment of replicated sister chromatids to the mitotic spindle apparatus. This study reveals an important role for the yeast RSC ATP-dependent chromatin-remodeling complex at the kinetochore in chromosome transmission. Mutations in genes encoding two core subunits of RSC, the ATPase Sth1p and the Snf5p homolog Sfh1p, interact genetically with mutations in genes encoding kinetochore proteins and with a mutation in centromeric DNA. RSC also interacts genetically and physically with the histone and histone variant components of centromeric chromatin. Importantly, RSC is localized to centromeric and centromere-proximal chromosomal regions, and its association with these loci is dependent on Sth1p. Both sth1 and sfh1 mutants exhibit altered centromeric and centromere-proximal chromatin structure and increased missegregation of authentic chromosomes. Finally, RSC is not required for centromeric deposition of the histone H3 variant Cse4p, suggesting that RSC plays a role in reconfiguring centromeric and flanking nucleosomes following Cse4p recruitment for proper chromosome transmission.

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Soutourina, Julie, Véronique Bordas-Le Floch, Gabrielle Gendrel, Amando Flores, Cécile Ducrot, Hélène Dumay-Odelot, Pascal Soularue, et al. "Rsc4 Connects the Chromatin Remodeler RSC to RNA Polymerases." Molecular and Cellular Biology 26, no. 13 (July 1, 2006): 4920–33. http://dx.doi.org/10.1128/mcb.00415-06.

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ABSTRACT RSC is an essential, multisubunit chromatin remodeling complex. We show here that the Rsc4 subunit of RSC interacted via its C terminus with Rpb5, a conserved subunit shared by all three nuclear RNA polymerases (Pol). Furthermore, the RSC complex coimmunoprecipitated with all three RNA polymerases. Mutations in the C terminus of Rsc4 conferred a thermosensitive phenotype and the loss of interaction with Rpb5. Certain thermosensitive rpb5 mutations were lethal in combination with an rsc4 mutation, supporting the physiological significance of the interaction. Pol II transcription of ca. 12% of the yeast genome was increased or decreased twofold or more in a rsc4 C-terminal mutant. The transcription of the Pol III-transcribed genes SNR6 and RPR1 was also reduced, in agreement with the observed localization of RSC near many class III genes. Rsc4 C-terminal mutations did not alter the stability or assembly of the RSC complex, suggesting an impact on Rsc4 function. Strikingly, a C-terminal mutation of Rsc4 did not impair RSC recruitment to the RSC-responsive genes DUT1 and SMX3 but rather changed the chromatin accessibility of DNases to their promoter regions, suggesting that the altered transcription of DUT1 and SMX3 was the consequence of altered chromatin remodeling.

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Lia, Giuseppe, Elise Praly, Helder Ferreira, Chris Stockdale, Yuk Ching Tse-Dinh, David Dunlap, Vincent Croquette, David Bensimon, and Tom Owen-Hughes. "Direct Observation of DNA Distortion by the RSC Complex." Molecular Cell 21, no. 3 (February 2006): 417–25. http://dx.doi.org/10.1016/j.molcel.2005.12.013.

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Shim, Eun Yong, Jia-Lin Ma, Ji-Hyun Oum, Yvonne Yanez, and Sang Eun Lee. "The Yeast Chromatin Remodeler RSC Complex Facilitates End Joining Repair of DNA Double-Strand Breaks." Molecular and Cellular Biology 25, no. 10 (May 15, 2005): 3934–44. http://dx.doi.org/10.1128/mcb.25.10.3934-3944.2005.

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ABSTRACT Repair of chromosome double-strand breaks (DSBs) is central to cell survival and genome integrity. Nonhomologous end joining (NHEJ) is the major cellular repair pathway that eliminates chromosome DSBs. Here we report our genetic screen that identified Rsc8 and Rsc30, subunits of the Saccharomyces cerevisiae chromatin remodeling complex RSC, as novel NHEJ factors. Deletion of RSC30 gene or the C-terminal truncation of RSC8 impairs NHEJ of a chromosome DSB created by HO endonuclease in vivo. rsc30Δ maintains a robust level of homologous recombination and the damage-induced cell cycle checkpoints. By chromatin immunoprecipitation, we show recruitment of RSC to a chromosome DSB with kinetics congruent with its involvement in NHEJ. Recruitment of RSC to a DSB depends on Mre11, Rsc30, and yKu70 proteins. Rsc1p and Rsc2p, two other RSC subunits, physically interact with yKu80p and Mre11p. The interaction of Rsc1p with Mre11p appears to be vital for survival from genotoxic stress. These results suggest that chromatin remodeling by RSC is important for NHEJ.

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Sing, Tina L., Minnie P. Hung, Shinsuke Ohnuki, Godai Suzuki, Bryan-Joseph San Luis, Melainia McClain, Jay R. Unruh, et al. "The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion." Journal of Cell Biology 217, no. 7 (June 6, 2018): 2445–62. http://dx.doi.org/10.1083/jcb.201709009.

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Ploidy is tightly regulated in eukaryotic cells and is critical for cell function and survival. Cells coordinate multiple pathways to ensure replicated DNA is segregated accurately to prevent abnormal changes in chromosome number. In this study, we characterize an unanticipated role for the Saccharomyces cerevisiae “remodels the structure of chromatin” (RSC) complex in ploidy maintenance. We show that deletion of any of six nonessential RSC genes causes a rapid transition from haploid to diploid DNA content because of nondisjunction events. Diploidization is accompanied by diagnostic changes in cell morphology and is stably maintained without further ploidy increases. We find that RSC promotes chromosome segregation by facilitating spindle pole body (SPB) duplication. More specifically, RSC plays a role in distributing two SPB insertion factors, Nbp1 and Ndc1, to the new SPB. Thus, we provide insight into a role for a SWI/SNF family complex in SPB duplication and ploidy maintenance.

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Rawal, Yashpal, Hongfang Qiu, and Alan G. Hinnebusch. "Distinct functions of three chromatin remodelers in activator binding and preinitiation complex assembly." PLOS Genetics 18, no. 7 (July 6, 2022): e1010277. http://dx.doi.org/10.1371/journal.pgen.1010277.

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The nucleosome remodeling complexes (CRs) SWI/SNF, RSC, and Ino80C cooperate in evicting or repositioning nucleosomes to produce nucleosome depleted regions (NDRs) at the promoters of many yeast genes induced by amino acid starvation. We analyzed mutants depleted of the catalytic subunits of these CRs for binding of transcriptional activator Gcn4 and recruitment of TATA-binding protein (TBP) during preinitiation complex (PIC) assembly. RSC and Ino80 were found to enhance Gcn4 binding to both UAS elements in NDRs upstream of promoters and to unconventional binding sites within nucleosome-occupied coding sequences; and SWI/SNF contributes to UAS binding when RSC is depleted. All three CRs are actively recruited by Gcn4 to most UAS elements and appear to enhance Gcn4 binding by reducing nucleosome occupancies at the binding motifs, indicating a positive regulatory loop. SWI/SNF acts unexpectedly in WT cells to prevent excessive Gcn4 binding at many UAS elements, indicating a dual mode of action that is modulated by the presence of RSC. RSC and SWI/SNF collaborate to enhance TBP recruitment at Gcn4 target genes, together with Ino80C, in a manner associated with nucleosome eviction at the TBP binding sites. Cooperation among the CRs in TBP recruitment is also evident at the highly transcribed ribosomal protein genes, while RSC and Ino80C act more broadly than SWI/SNF at the majority of other constitutively expressed genes to stimulate this step in PIC assembly. Our findings indicate a complex interplay among the CRs in evicting promoter nucleosomes to regulate activator binding and stimulate PIC assembly.

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Goodwin, G. H., and R. H. Nicolas. "The BAH domain, polybromo and the RSC chromatin remodelling complex." Gene 268, no. 1-2 (May 2001): 1–7. http://dx.doi.org/10.1016/s0378-1119(01)00428-0.

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13

Andress, Edward J., Roman Holic, Mariola J. Edelmann, Benedikt M. Kessler, and Veronica P. C. C. Yu. "Dia2 Controls Transcription by Mediating Assembly of the RSC Complex." PLoS ONE 6, no. 6 (June 20, 2011): e21172. http://dx.doi.org/10.1371/journal.pone.0021172.

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14

Kent, Nicholas A., Anna L. Chambers, and Jessica A. Downs. "Dual Chromatin Remodeling Roles for RSC during DNA Double Strand Break Induction and Repair at the Yeast MAT Locus." Journal of Biological Chemistry 282, no. 38 (July 25, 2007): 27693–701. http://dx.doi.org/10.1074/jbc.m704707200.

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DNA double strand breaks (DSBs) are potentially serious chromosomal lesions. However, cells sometimes deliberately cleave their own DNA to facilitate certain chromosomal processes, and there is much interest in how such self-inflicted breaks are effectively managed. Eukaryotic DSBs occur in the context of chromatin and the RSC chromatin-remodeling ATPase complex has been shown to promote DSB repair at the budding yeast MAT locus DSB, created by the HO endonuclease during mating type switching. We show that the role of RSC at MAT is highly specialized. The Rsc1p subunit of RSC directs nucleosome sliding immediately after DSB creation at both MAT and generally and is required for efficient DNA damage-induced histone H2A phosphorylation and strand resection during repair by homologous recombination. However, the Rsc2p and Rsc7p subunits are additionally required to set up a basal MAT locus structure. This RSC-dependent chromatin structure at MAT ensures accessibility to the HO endonuclease. The RSC complex therefore has chromatin remodeling roles both before and after DSB induction at MAT, promoting both DNA cleavage and subsequent repair.

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Lorch, Yahli, Bradley R. Cairns, Mincheng Zhang, and Roger D. Kornberg. "Activated RSC–Nucleosome Complex and Persistently Altered Form of the Nucleosome." Cell 94, no. 1 (July 1998): 29–34. http://dx.doi.org/10.1016/s0092-8674(00)81218-0.

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Chaban, Yuriy, Chukwudi Ezeokonkwo, Wen-Hsiang Chung, Fan Zhang, Roger D. Kornberg, Barbara Maier-Davis, Yahli Lorch, and Francisco J. Asturias. "Structure of a RSC–nucleosome complex and insights into chromatin remodeling." Nature Structural & Molecular Biology 15, no. 12 (November 23, 2008): 1272–77. http://dx.doi.org/10.1038/nsmb.1524.

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Lorch, Yahli, Joachim Griesenbeck, Hinrich Boeger, Barbara Maier-Davis, and Roger D. Kornberg. "Selective removal of promoter nucleosomes by the RSC chromatin-remodeling complex." Nature Structural & Molecular Biology 18, no. 8 (July 3, 2011): 881–85. http://dx.doi.org/10.1038/nsmb.2072.

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Floer, Monique, Xin Wang, Vidya Prabhu, Georgina Berrozpe, Santosh Narayan, Dan Spagna, David Alvarez, et al. "A RSC/Nucleosome Complex Determines Chromatin Architecture and Facilitates Activator Binding." Cell 141, no. 3 (April 2010): 407–18. http://dx.doi.org/10.1016/j.cell.2010.03.048.

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Balachandra, Vinutha K., Jiyoti Verma, Madhu Shankar, Timothy M. Tucey, Ana Traven, Ralf B. Schittenhelm, and Santanu K. Ghosh. "The RSC (Remodels the Structure of Chromatin) complex of Candida albicans shows compositional divergence with distinct roles in regulating pathogenic traits." PLOS Genetics 16, no. 11 (November 5, 2020): e1009071. http://dx.doi.org/10.1371/journal.pgen.1009071.

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Regulation of gene expression programs is crucial for the survival of microbial pathogens in host environments and for their ability to cause disease. Here we investigated the epigenetic regulator RSC (Remodels the Structure of Chromatin) in the most prevalent human fungal pathogen Candida albicans. Biochemical analysis showed that CaRSC comprises 13 subunits and contains two novel non-essential members, which we named Nri1 and Nri2 (Novel RSC Interactors) that are exclusive to the CTG clade of Saccharomycotina. Genetic analysis showed distinct essentiality of C. albicans RSC subunits compared to model fungal species suggesting functional and structural divergence of RSC functions in this fungal pathogen. Transcriptomic and proteomic profiling of a conditional mutant of the essential catalytic subunit gene STH1 demonstrated global roles of RSC in C. albicans biology, with the majority of growth-related processes affected, as well as mis-regulation of genes involved in morphotype switching, host-pathogen interaction and adaptive fitness. We further assessed the functions of non-essential CaRSC subunits, showing that the novel subunit Nri1 and the bromodomain subunit Rsc4 play roles in filamentation and stress responses; and also interacted at the genetic level to regulate cell viability. Consistent with these roles, Rsc4 is required for full virulence of C. albicans in the murine model of systemic infection. Taken together, our data builds the first comprehensive study of the composition and roles of RSC in C. albicans, showing both conserved and distinct features compared to model fungal systems. The study illuminates how C. albicans uses RSC-dependent transcriptional regulation to respond to environmental signals and drive survival fitness and virulence in mammals.

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Sadurski, Leszek. "Regional Security Complex Theory: Why Is this Concept Still Worth Developing?" Athenaeum Polskie Studia Politologiczne 75, no. 3 (2022): 137–53. http://dx.doi.org/10.15804/athena.2022.75.08.

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The Theory of Regional Security Complexes (RSC) provides a conceptual framework to encompass the emerging new post-Cold War international security order. It proposes a model of regional security which makes it possible to analyse, explain and predict the development of the situation in a given region. It is based on the assumption that it is the regional level, not the global or the level of a single state, that constitutes the optimal basis for conducting security analyses. So far, few researchers have attempted to challenge the theoretical assumptions of the RSC concept, and few have tried to develop or supplement it. At the same time, it is clear that the emerging new types of challenges, changing the state’s behaviour, force the revision or updating of some existing theoretical frameworks. It also applies to the Regional Security Complex theory and the adaptation of its conceptual framework to the surrounding reality so that it can continue to be reliably studied. This article considers the possibilities of further evolution of the RSC theory and, in particular, analyses the state of its development to date and proposes solutions to complement it and adapt it to newly emerging phenomena.

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Prasad, Rashmi, Sheena D'Arcy, Arjan Hada, Karolin Luger, and Blaine Bartholomew. "Coordinated Action of Nap1 and RSC in Disassembly of Tandem Nucleosomes." Molecular and Cellular Biology 36, no. 17 (June 6, 2016): 2262–71. http://dx.doi.org/10.1128/mcb.00195-16.

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The SWI/SNF and RSC family of ATP-dependent chromatin remodelers disassembles nucleosomes by moving nucleosomes into the vicinity of adjoining nucleosomes. We found that the histone chaperone Nap1 efficiently promotes disassembly of adjacent nucleosomes with which RSC collides and not the disassembly of nucleosomes mobilized by RSC. Nap1 is specific to RSC, as it does not target SWI/SNF, its paralog inSaccharomyces cerevisiae. Extensive mutational analysis of Nap1 has revealed that Nap1 affinity for histones H2A-H2B and H3-H4 and its ability to displace histones from DNA are required for Nap1 to enhance RSC-mediated disassembly. Other histone chaperones, such as Vps75, that also bind histones are not able to enhance RSC-mediated disassembly. Our study suggests a mechanism by which Nap1 is recruited to actively transcribed regions and assists in the passage of the transcription complex through chromatin, and it provides a novel mechanism for the coordinated action of RSC and Nap1.

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Baetz, Kristin K., Nevan J. Krogan, Andrew Emili, Jack Greenblatt, and Philip Hieter. "The ctf13-30/CTF13 Genomic Haploinsufficiency Modifier Screen Identifies the Yeast Chromatin Remodeling Complex RSC, Which Is Required for the Establishment of Sister Chromatid Cohesion." Molecular and Cellular Biology 24, no. 3 (February 1, 2004): 1232–44. http://dx.doi.org/10.1128/mcb.24.3.1232-1244.2003.

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ABSTRACT The budding yeast centromere-kinetochore complex ensures high-fidelity chromosome segregation in mitosis and meiosis by mediating the attachment and movement of chromosomes along spindle microtubules. To identify new genes and pathways whose function impinges on chromosome transmission, we developed a genomic haploinsufficiency modifier screen and used ctf13-30, encoding a mutant core kinetochore protein, as the reference point. We demonstrate through a series of secondary screens that the genomic modifier screen is a successful method for identifying genes that encode nonessential proteins required for the fidelity of chromosome segregation. One gene isolated in our screen was RSC2, a nonessential subunit of the RSC chromatin remodeling complex. rsc2 mutants have defects in both chromosome segregation and cohesion, but the localization of kinetochore proteins to centromeres is not affected. We determined that, in the absence of RSC2, cohesin could still associate with chromosomes but fails to achieve proper cohesion between sister chromatids, indicating that RSC has a role in the establishment of cohesion. In addition, numerous subunits of RSC were affinity purified and a new component of RSC, Rtt102, was identified. Our work indicates that only a subset of the nonessential RSC subunits function in maintaining chromosome transmission fidelity.

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Marsai, Viktor. "Csodás barátságok kezdete." Afrika Tanulmányok / Hungarian Journal of African Studies 13, no. 1-2. (August 20, 2019): 75–100. http://dx.doi.org/10.15170/at.2019.13.1-2.5.

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Nowadays the East African Regional Security Complex (RSC) is going trough a radical reshaping: we can observe an increasing interdependency among the countries of the region and the expanding boundaries of the RSC which are deeply affected by the political and security processes of the Arabian Peninsula, North and Southern-East Africa. Although it is too early to declare the birth of a new RSC, it is obvious that the original geographic frameworks of the analysis are not sustainable any more.

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Akbarzadeh, Mohammad M. "Correction: Nano-micellar Zn(Cys)2 complex mimics the chloroperoxidase active site." RSC Advances 6, no. 32 (2016): 26883–84. http://dx.doi.org/10.1039/c6ra90027e.

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Ng, H. H. "Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex." Genes & Development 16, no. 7 (April 1, 2002): 806–19. http://dx.doi.org/10.1101/gad.978902.

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Imamura, Yuko, Feifei Yu, Misaki Nakamura, Yuhki Chihara, Kyo Okane, Masahiro Sato, Muneyoshi Kanai, et al. "RSC Chromatin-Remodeling Complex Is Important for Mitochondrial Function in Saccharomyces cerevisiae." PLOS ONE 10, no. 6 (June 18, 2015): e0130397. http://dx.doi.org/10.1371/journal.pone.0130397.

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Titus, Laura C., T. Renee Dawson, Deborah J. Rexer, Kathryn J. Ryan, and Susan R. Wente. "Members of the RSC Chromatin-Remodeling Complex Are Required for Maintaining Proper Nuclear Envelope Structure and Pore Complex Localization." Molecular Biology of the Cell 21, no. 6 (March 15, 2010): 1072–87. http://dx.doi.org/10.1091/mbc.e09-07-0615.

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The assembly, distribution, and functional integrity of nuclear pore complexes (NPCs) in the nuclear envelope (NE) are key determinants in the nuclear periphery architecture. However, the mechanisms controlling proper NPC and NE structure are not fully defined. We used two different genetic screening approaches to identify Saccharomyces cerevisiae mutants with defects in NPC localization. The first approach examined green fluorescent protein (GFP)-Nic96 in 531 strains from the yeast Tet-promoters Hughes Collection with individual essential genes expressed from a doxycycline-regulated promoter (TetO7-orf). Under repressive conditions, depletion of the protein encoded by 44 TetO7-orf strains resulted in mislocalized GFP-Nic96. These included STH1, RSC4, RSC8, RSC9, RSC58, ARP7, and ARP9, each encoding components of the RSC chromatin remodeling complex. Second, a temperature-sensitive sth1-F793S (npa18-1) mutant was identified in an independent genetic screen for NPC assembly (npa) mutants. NPC mislocalization in the RSC mutants required new protein synthesis and ongoing transcription, confirming that lack of global transcription did not underlie the phenotypes. Electron microscopy studies showed significantly altered NEs and nuclear morphology, with coincident cytoplasmic membrane sheet accumulation. Strikingly, increasing membrane fluidity with benzyl alcohol treatment prevented the sth1-F793S NE structural defects and NPC mislocalization. We speculate that NE structure is functionally linked to proper chromatin architecture.

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Muñoz, Sofía, Francesca Passarelli, and Frank Uhlmann. "Conserved roles of chromatin remodellers in cohesin loading onto chromatin." Current Genetics 66, no. 5 (April 10, 2020): 951–56. http://dx.doi.org/10.1007/s00294-020-01075-x.

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Abstract Cohesin is a conserved, ring-shaped protein complex that topologically entraps DNA. This ability makes this member of the structural maintenance of chromosomes (SMC) complex family a central hub of chromosome dynamics regulation. Besides its essential role in sister chromatid cohesion, cohesin shapes the interphase chromatin domain architecture and plays important roles in transcriptional regulation and DNA repair. Cohesin is loaded onto chromosomes at centromeres, at the promoters of highly expressed genes, as well as at DNA replication forks and sites of DNA damage. However, the features that determine these binding sites are still incompletely understood. We recently described a role of the budding yeast RSC chromatin remodeler in cohesin loading onto chromosomes. RSC has a dual function, both as a physical chromatin receptor of the Scc2/Scc4 cohesin loader complex, as well as by providing a nucleosome-free template for cohesin loading. Here, we show that the role of RSC in sister chromatid cohesion is conserved in fission yeast. We discuss what is known about the broader conservation of the contribution of chromatin remodelers to cohesin loading onto chromatin.

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Razgon, Margarita, and Alireza Mousavi. "Relaxed Rule-Based Learning for Automated Predictive Maintenance: Proof of Concept." Algorithms 13, no. 9 (September 3, 2020): 219. http://dx.doi.org/10.3390/a13090219.

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In this paper we propose a novel approach of rule learning called Relaxed Separate-and- Conquer (RSC): a modification of the standard Separate-and-Conquer (SeCo) methodology that does not require elimination of covered rows. This method can be seen as a generalization of the methods of SeCo and weighted covering that does not suffer from fragmentation. We present an empirical investigation of the proposed RSC approach in the area of Predictive Maintenance (PdM) of complex manufacturing machines, to predict forthcoming failures of these machines. In particular, we use for experiments a real industrial case study of a Continuous Compression Moulding (CCM) machine which manufactures the plastic bottle closure (caps) in the beverage industry. We compare the RSC approach with a Decision Tree (DT) based and SeCo algorithms and demonstrate that RSC significantly outperforms both DT based and SeCo rule learners. We conclude that the proposed RSC approach is promising for PdM guided by rule learning.

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Vasylchenko, V., А. Koliadiuk, V. Posokh, and V. Dubkovsky. "Determination of Stiffness Characteristics for WWER-1000 Support Components." Nuclear and Radiation Safety, no. 4(84) (December 19, 2019): 12–17. http://dx.doi.org/10.32918/nrs.2019.4(84).02.

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Available approaches to computer justification of safe operation of reactor support components (RSC) are generally presented in the reports justifying RSC lifetime extension at Ukrainian NPPs. The experience of performing such calculations and analyzing the results indicates that there are issues to be improved. Thus, in particular, RSC stress-strain state is not analyzed for all force factors in the available calculations. It is typical for the reports on RSC lifetime extension justification that the strength assessment of the RSC is performed in an axisymmetric formulation (1/30). Meanwhile, a complex asymmetrical load from the main circulation pipelines is not considered, which affects RSC stress-strain state calculations as some stiffness characteristics (horizontal and torsional stiffness) cannot be determined correctly in a 1/30 axisymmetric formulation. The paper proposes a methodology for determining RSC stiffness in a complete formulation (without applying symmetry conditions), taking into account all geometric features and the interaction between individual structures and nodes of RSC. Thus, for each RSC (support ring, thrust ring, separating bellows), based on geometric features and types of connection to other components, stiffness to be determined to calculate the forces is defined (six for support ring and separating bellows and three for thrust ring respectively). Single loads in the form of forces and moments were imposed on the remote points connected to corresponding RSC surfaces to determine stiffness. This load made it possible to obtain the displacements and rotation angles of the corresponding remote points, which were used to calculate the stiffness characteristics. The described approach was first used in the calculations performed within justification of KhNPP Unit 1.

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Cao, Y., B. R. Cairns, R. D. Kornberg, and B. C. Laurent. "Sfh1p, a component of a novel chromatin-remodeling complex, is required for cell cycle progression." Molecular and Cellular Biology 17, no. 6 (June 1997): 3323–34. http://dx.doi.org/10.1128/mcb.17.6.3323.

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Several eukaryotic multiprotein complexes, including the Saccharomyces cerevisiae Snf/Swi complex, remodel chromatin for transcription. In contrast to the Snf/Swi proteins, Sfh1p, a new Snf5p paralog, is essential for viability. The evolutionarily conserved domain of Sfh1p is sufficient for normal function, and Sfh1p interacts functionally and physically with an essential Snf2p paralog in a novel nucleosome-restructuring complex called RSC (for remodels the structure of chromatin). A temperature-sensitive sfh1 allele arrests cells in the G2/M phase of the cell cycle, and the Sfh1 protein is specifically phosphorylated in the G1 phase. Together, these results demonstrate a link between chromatin remodeling and progression through the cell division cycle, providing genetic clues to possible targets for RSC function.

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Chai, Bob, Jing-mei Hsu, Jian Du, and Brehon C. Laurent. "Yeast RSC Function Is Required for Organization of the Cellular Cytoskeleton via an Alternative PKC1 Pathway." Genetics 161, no. 2 (June 1, 2002): 575–84. http://dx.doi.org/10.1093/genetics/161.2.575.

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Abstract RSC is a 15-protein ATP-dependent chromatin-remodeling complex related to Snf-Swi, the prototypical ATP-dependent nucleosome remodeler in budding yeast. Despite insight into the mechanism by which purified RSC remodels nucleosomes, little is known about the chromosomal targets or cellular pathways in which RSC acts. To better understand the cellular function of RSC, a screen was undertaken for gene dosage suppressors of sth1-3ts, a temperature-sensitive mutation in STH1, which encodes the essential ATPase subunit. Slg1p and Mid2p, two type I transmembrane stress sensors of cell wall integrity that function upstream of protein kinase C (Pkc1p), were identified as multicopy suppressors of sth1-3ts cells. Although the sth1-3ts mutant exhibits defects characteristic of PKC1 pathway mutants (caffeine and staurosporine sensitivities and an osmoremedial phenotype), only upstream components and not downstream effectors of the PKC1-MAP kinase pathway can suppress defects conferred by sth1-3ts, suggesting that RSC functions in an alternative PKC1-dependent pathway. Moreover, sth1-3ts cells display defects in actin cytoskeletal rearrangements and are hypersensitive to the microtubule depolymerizing drug, TBZ; both of these defects can be corrected by the high-copy suppressors. Together, these data reveal an important functional connection between the RSC remodeler and PKC1-dependent signaling in regulating the cellular architecture.

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Mu, Dong, Haonan Ni, and Huanyu Ren. "Structural Characteristics of EV Li-ion Batteries Recycling Supply Chain Network." Journal of Physics: Conference Series 2160, no. 1 (January 1, 2022): 012042. http://dx.doi.org/10.1088/1742-6596/2160/1/012042.

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Abstract With the rapid increase in the decommissioning of the EV Li-ion batteries (EV-LIBs), the EV-LIBs recycling industry is growing dramatically. Based on the realistic supply relationship data of major firms in the EV-LIBs recycling industry, this paper uses the complex network theory to construct a global firm-level EV-LIBs recycling supply chain (EV-LIBs-RSC) network and analyze the structural characteristics of the EV-LIBs-RSC network from the network level. Specifically, The degree distribution, average degree, overall density, average shortest path length, and community structure of EV-LIBs-RSC network are systematically analyzed. The relevant results can assist the government in proposing appropriate industrial policies.

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Huang, Jian, Jing-mei Hsu, and Brehon C. Laurent. "The RSC Nucleosome-Remodeling Complex Is Required for Cohesin's Association With Chromosome Arms." Molecular Cell 13, no. 5 (March 2004): 739–50. http://dx.doi.org/10.1016/s1097-2765(04)00103-0.

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Huang, Jian, Jing-mei Hsu, and Brehon C. Laurent. "The RSC Nucleosome-Remodeling Complex Is Required for Cohesin's Association with Chromosome Arms." Molecular Cell 15, no. 2 (July 2004): 315. http://dx.doi.org/10.1016/j.molcel.2004.07.006.

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Czaja, Wioletta, Peng Mao, and Michael J. Smerdon. "Chromatin remodelling complex RSC promotes base excision repair in chromatin of Saccharomyces cerevisiae." DNA Repair 16 (April 2014): 35–43. http://dx.doi.org/10.1016/j.dnarep.2014.01.002.

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Skiniotis, Georgios, Danesh Moazed, and Thomas Walz. "Acetylated Histone Tail Peptides Induce Structural Rearrangements in the RSC Chromatin Remodeling Complex." Journal of Biological Chemistry 282, no. 29 (May 29, 2007): 20804–8. http://dx.doi.org/10.1074/jbc.c700081200.

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Moreira, Jose´ M. A., and Steen Holmberg. "Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC." EMBO Journal 18, no. 10 (May 17, 1999): 2836–44. http://dx.doi.org/10.1093/emboj/18.10.2836.

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Wu, Andrew CK, Claudia Vivori, Harshil Patel, Theodora Sideri, Fabien Moretto, and Folkert J. van Werven. "RSC and GRFs confer promoter directionality by restricting divergent noncoding transcription." Life Science Alliance 5, no. 12 (September 16, 2022): e202201394. http://dx.doi.org/10.26508/lsa.202201394.

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The directionality of gene promoters—the ratio of protein-coding over divergent noncoding transcription—is highly variable. How promoter directionality is controlled remains poorly understood. Here, we show that the chromatin remodelling complex RSC and general regulatory factors (GRFs) dictate promoter directionality by attenuating divergent transcription relative to protein-coding transcription. At gene promoters that are highly directional, depletion of RSC leads to a relative increase in divergent noncoding transcription and thus to a decrease in promoter directionality. We find that RSC has a modest effect on nucleosome positioning upstream in promoters at the sites of divergent transcription. These promoters are also enriched for the binding of GRFs such as Reb1 and Abf1. Ectopic targeting of divergent transcription initiation sites with GRFs or the dCas9 DNA-binding protein suppresses divergent transcription. Our data suggest that RSC and GRFs play a pervasive role in limiting divergent transcription relative to coding direction transcription. We propose that any DNA-binding factor, when stably associated with cryptic transcription start sites, forms a barrier which represses divergent transcription, thereby promoting promoter directionality.

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LAKE, DAVID A. "Regional hierarchy: authority and local international order." Review of International Studies 35, S1 (February 2009): 35–58. http://dx.doi.org/10.1017/s0260210509008420.

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A regional security complex (RSC) is a set of states continuously affected by one or more security externalities that emanate from a distinct geographic area. In such a complex, the members are so interrelated in terms of their security that actions by any one member, and significant security-related developments inside any member, have a major impact on others. Regional orders describe how states within an RSC manage their security relations and range from balances of power, to regional power concerts, collective security organisations, pluralistic security communities, and integration. As regional states move ‘up’ this continuum of regional orders, relations generally become more peaceful and interactions less coloured by actual or threatened violence.

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Fatima, Noshin, Fakhra Aziz, Zubair Ahmad, M. A. Najeeb, M. I. Azmeer, Kh S. Karimov, M. M. Ahmed, S. Bashir, R. A. Shakoor, and K. Sulaiman. "Correction: Compositional engineering of the pi-conjugated small molecular VOPcPhO : Alq3 complex to boost humidity sensing." RSC Advances 7, no. 41 (2017): 25621. http://dx.doi.org/10.1039/c7ra90062g.

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Khan, Aamir A., Susan K. Fullerton-Shirey, and Scott S. Howard. "Correction: Easily prepared ruthenium-complex nanomicelle probes for two-photon quantitative imaging of oxygen in aqueous media." RSC Advances 5, no. 8 (2015): 6131. http://dx.doi.org/10.1039/c4ra90059f.

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Yang, Fan, Guangqing Chi, Ge Wang, Shirui Tang, Yunting Li, and Cong Ju. "Untangle the Complex Stakeholder Relationships in Rural Settlement Consolidation in China: A Social Network Approach." Land 9, no. 7 (June 29, 2020): 210. http://dx.doi.org/10.3390/land9070210.

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Rural settlement consolidation (RSC) has a critical role in facilitating the transformation of human settlement and land use transition in the rural revitalization process. RSC involves a diversity of stakeholder groups with complex and intertwined concerns. It is therefore crucial to identify the key stakeholders and their main concerns to effectively align rural planning and policymaking. However, this line of research remains underdeveloped. This study provides a novel and holistic network perspective for unpacking the complex relationships among different stakeholders. The results indicate: (1) the network of stakeholder concerns is relatively sparse, with 68 concern nodes and 159 concern ties; (2) The village committee, centralized residents, and contractors occupy the core position within the concerns network, while the local government has the majority of strongly connected nodes; (3) The lists of prominent concern nodes and ties are identified by different network indices, including the degree difference, the out-status centrality, closeness centrality, node betweenness centrality, and link betweenness centrality; (4) The main interaction type among stakeholder groups can be classified into five categories: financing, psychological attachment, stakeholder participation, project management, and the improvement in living conditions and infrastructure. This study reveals the relatively weak status of residents, the pivotal role of the village committee, as well as the indispensable part of the contractor and township government, with the aim to provide targeted guidance and decision-making supports for strengthening interactions and cooperation among different stakeholder groups. The findings shed new light on performing the multi-tasks of RSC and facilitating the sustainable management of rural areas.

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Helmer, Dorothea, Ina Rink, James A. R. Dalton, Kevin Brahm, Marina Jöst, Tobias M. Nargang, Witali Blum, et al. "Correction: Rational design of a peptide capture agent for CXCL8 based on a model of the CXCL8:CXCR1 complex." RSC Advances 8, no. 30 (2018): 16800–16801. http://dx.doi.org/10.1039/c8ra90035c.

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Singh Mehata, Mohan, Yang Yang, Zong-Jin Qu, Jun-Sheng Chen, Feng-Jiao Zhao, and Ke-Li Han. "Correction: Spin mixed charge transfer states of iridium complex Ir(ppy)3: transient absorption and time-resolved photoluminescence." RSC Advances 5, no. 92 (2015): 75210. http://dx.doi.org/10.1039/c5ra90082d.

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Correction for ‘Spin mixed charge transfer states of iridium complex Ir(ppy)₃: transient absorption and time-resolved photoluminescence’ by Mohan Singh Mehata et al., RSC Adv., 2015, 5, 34094–34099.

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Spain, Marla M., Suraiya A. Ansari, Rakesh Pathak, Michael J. Palumbo, Randall H. Morse, and Chhabi K. Govind. "The RSC Complex Localizes to Coding Sequences to Regulate Pol II and Histone Occupancy." Molecular Cell 56, no. 5 (December 2014): 653–66. http://dx.doi.org/10.1016/j.molcel.2014.10.002.

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Chow, Sih Yao. "Establishing Real-Time, Single-Molecule Approaches to Study RSC Complex Mediated Nucleosome Remodeling Activities." Biophysical Journal 104, no. 2 (January 2013): 580a. http://dx.doi.org/10.1016/j.bpj.2012.11.3222.

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Klein-Brill, Avital, Daphna Joseph-Strauss, Alon Appleboim, and Nir Friedman. "Dynamics of Chromatin and Transcription during Transient Depletion of the RSC Chromatin Remodeling Complex." Cell Reports 26, no. 1 (January 2019): 279–92. http://dx.doi.org/10.1016/j.celrep.2018.12.020.

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KOYAMA, Hirofumi, Taka-aki NAGAO, Tomomi INAI, Kohji MIYAHARA, Yasufumi HAYASIDA, Katsuhiko SHIRAHIGE, and Eiko TSUCHIYA. "RSC Nucleosome-remodeling Complex Plays Prominent Roles in Transcriptional Regulation throughout Budding Yeast Gametogenesis." Bioscience, Biotechnology, and Biochemistry 68, no. 4 (January 2004): 909–19. http://dx.doi.org/10.1271/bbb.68.909.

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Krishnasamy, Lalitha, Rajesh Kumar Dhanaraj, D. Ganesh Gopal, Thippa Reddy Gadekallu, Mohamed K. Aboudaif, and Emad Abouel Nasr. "A Heuristic Angular Clustering Framework for Secured Statistical Data Aggregation in Sensor Networks." Sensors 20, no. 17 (August 31, 2020): 4937. http://dx.doi.org/10.3390/s20174937.

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Clustering in wireless sensor networks plays a vital role in solving energy and scalability issues. Although multiple deployment structures and cluster shapes have been implemented, they sometimes fail to produce the expected outcomes owing to different geographical area shapes. This paper proposes a clustering algorithm with a complex deployment structure called radial-shaped clustering (RSC). The deployment structure is divided into multiple virtual concentric rings, and each ring is further divided into sectors called clusters. The node closest to the midpoint of each sector is selected as the cluster head. Each sector’s data are aggregated and forwarded to the sink node through angular inclination routing. We experimented and compared the proposed RSC performance against that of the existing fan-shaped clustering algorithm. Experimental results reveal that RSC outperforms the existing algorithm in scalability and network lifetime for large-scale sensor deployments.

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Journal articles on the topic 'RSC complex'

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