Dissertations / Theses on the topic 'Rootstock'

Grapevine is worldwide grafted on rootstocks to create a biological barrier to the phylloxera (Daktulosphaira vitifoliae). Despite the key role of rootstock in the adaptation to environmental conditions, a limited number of genotypes is available for winegrowers, showing a narrow genetic background. The gap between the importance of rootstocks in abiotic stress tolerance and their low genetic variability leads to consider rootstock breeding as a promising strategy to face climate change. In the last decades, new breeding programs were developed with the aim to provide new rootstocks able to cope with drought and other abiotic stresses. Nowadays, the continuous progress in genetic techniques can assist and accelerate the selection process of new tolerant genotypes. In the present PhD project, several genotypes at different stages in rootstock selection process were analyzed for drought tolerance. The first part of the thesis focused on 3 genotypes belonging to the recent M-series, the second part was about a new selection of 30 genotypes, coming from different breeding programs, and in the last part a breeding population of 141 genotypes was used for a genome wide association study (GWAS). The new M-rootstocks (M1, M3 and M4), recently placed on the market, were compared to traditional rootstocks, in order to better understand their behavior under drought. In a pot experiment under controlled conditions, M1, M3 and M4 were compared to nine rootstocks with different genetic background at decreasing levels of water availability. M-rootstock performance under water deficit was similar to the tolerant rootstocks 1103P and 110R, in both phenotypic and genetic responses to water stress. These rootstocks adopted a strategy of tolerance to face water stress, increasing the water use efficiency (WUE) under deficit conditions. To deeply investigate the behavior of tolerant rootstocks under drought, a second experiment in semi-controlled conditions was set up, comparing M4 to 1103P under progressive water deficit, in grafting combination with V. vinifera cv Pinot Blanc. Similar performances were reported by the two grafting combinations under mild to moderate water deficit, but a different response occurred under sever conditions: 1103P reduced stomatal conductance, transpiration, and carbon assimilation more than M4, which was able to preserve water use efficiency and operating efficiency of photosystem II. In the second part of the thesis 30 new selected genotypes were compared to rootstock M2 for water stress tolerance and nutritional status, in order to characterize the rootstock material before the marketing process and to identify new pre-breeding material. The experiment was carried out in un-grafted conditions for two years and in two experimental fields, characterized by different water availability. Several parameters were analyzed, such as transpiration, WUE, vigor, macronutrients and micronutrients in the leaves. Genotypes ranked for both abiotic stresses and the differences between the two sites allowed to estimate their plasticity for each trait. Finally, a GWA approach was applied on a breeding population, counting 141 genotypes, in order to identify the genomic regions involved in drought tolerance. The population was genotyped with a 18k SNP array, after the validation on non-vinifera germplasm, belonging to a rootstock core-collection of 70 genotypes. Three phenotyping cycles under increasing water deficit were performed on the breeding population under greenhouse-controlled conditions. Vigor, shoot growth rate, transpiration, stomatal conductance and leaf turgor were estimated for each genotype at different water deficit levels. A group of tolerant genotypes with high performance under water deficit condition was identified and used in GWAS approach to detect the loci associated to drought tolerance of rootstocks. In conclusion, this work enhanced the knowledge about rootstock response to water deficit, characterized the water tolerance of a large panel of rootstocks and identified potential target genes for future breeding programs.

In a rootstock evaluation trial planted in 1993, five rootstocks, ‘Carrizo’ citrange, Citrus macrophylla, ‘Rough Lemon’, Swingle citrumelo and Citrus volkameriana were selected for evaluation using 'Limoneira 8A Lisbon' as the scion. 1994-2002 yield and packout results indicate that trees on C. macrophylla, C. volkameriana and ‘Rough Lemon’ are superior to those on other rootstocks in both growth and yield. C. macrophylla is outperforming C. volkameriana. For the second year in a row, ‘Rough Lemon’ trees performed similarly to C. macrophylla and better than C. volkameriana. ‘Swingle’ and Carrizo’ are performing poorly. In two other rootstock evaluation trials, both planted in 1995, C. macrophylla and/or C. volkameriana are outperforming other trifoliate and trifoliate-hybrid rootstocks under test.

In a rootstock evaluation trial planted in 1993, five rootstocks, ‘Carrizo’ citrange, Citrus macrophylla, ‘Rough Lemon’, Swingle citrumelo and Citrus volkameriana were selected for evaluation using 'Limoneira 8A Lisbon' as the scion. 1994-2005 yield and packout results indicate that trees on C. macrophylla, C. volkameriana and ‘Rough Lemon’ are superior to those on other rootstocks in both growth and yield. C. macrophylla is no longer outperforming C. volkameriana. ‘Swingle’ and Carrizo’ are performing poorly.

In a rootstock evaluation trial planted in 1993, five rootstocks, 'Carrizo' citrange, Citrus macrophylla, 'Rough Lemon', Swingle citrumelo and Citrus volkameriana were selected for evaluation using 'Limoneira 8A Lisbon' as the scion. 1994-2006 yield and packout results indicate that trees on C. macrophylla, C. volkameriana and ‘Rough Lemon’ are superior to those on other rootstocks in both growth and yield. C. macrophylla does not consistently outperform C. volkameriana. 'Swingle' and Carrizo’ are performing poorly.

In a rootstock evaluation trial planted in 1993, five rootstocks, 'Carrizo' citrange, Citrus macrophylla, 'Rough Lemon', Swingle citrumelo and Citrus volkameriana were selected for evaluation using 'Limoneira 8A Lisbon' as the scion. 1994-2002 yield and packout results indicate that trees on C. macrophylla, C. volkameriana and 'Rough Lemon' are superior to those on other rootstocks in both growth and yield. C. macrophylla is outperforming C. volkameriana. For the second year in a row, 'Rough Lemon' trees performed similarly to C. macrophylla and better than C. volkameriana. 'Swingle' and 'Carrizo' are performing poorly. In two other rootstock evaluation trials, both planted in 1995, C. macrophylla and/or C. volkameriana are outperforming other trifoliate and trifoliate-hybrid rootstocks under test.

Several differentially expressed markers of compatibility or incompatibility were isolated and are being molecularly characterized One marker is present in young Eureka on Macrophylla trees and on Macrophylla decline affected, Eureka on Macrophylla trees, while absent on healthy, Eureka on Macrophylla trees of the same combination. A second marker appears similar to a gene that encodes a Zn-binding homeodomain of a DNA binding protein in plant cells. This particular marker was found in the leaves of healthy trees, but absent in Macrophylla decline trees, which are known to be Zn deficient. Thirty-five markers are being characterized in all.

Thesis (MScAgric)--University of Stellenbosch, 2001.
ENGLISH ABSTRACT: Delayed foliation is commonly observed in apple and pear producing countries with warm and/or short winter climates, resulting in less synchronised budburst, of fewer buds and reduced yield. Applications of rest-breaking agents minimise the symptoms of delayed foliation. According to chilling models used in fruit producing areas of the world, the climate of South Africa is not suitable for deciduous fruit production, however fruit has been successfully produced here for a long time. This study aimed to quantify the effects of freezing temperatures, different chilling temperatures and the period of chilling, to obtain a better knowledge of bud dormancy release on apple and pear shoots. The chilling period was the most important factor influencing the progression of dormancy. While in some cases the chilling temperature and the freeze treatment effects were significant, the contribution to differences in the progression of dormancy was negligible. Our findings indicate that currently used chilling models should emphasise the time of exposure to low temperatures more than the difference in temperatures between I to ro-c. The South African apple and pear industry made good progress in moving towards high density plantings, but large variation in soil types, non-optimum growing conditions, replant situations and a lack experience with dwarfing rootstocks limited further development. However, there is sti II an urgency to obtain higher early yields of good quality fruit applying the most efficient production practices. We aimed to quantify the field performance of locally available apple and pear rootstocks, in particular from data outside of previously reported local trials, as well as early production of newly planted trials. Information obtained form the industry indicates that BPI and BP3 are the preferred pear rootstocks. From production records of 'Packham's Triumph' pears, it appeared that BP3 and OHxF97 produced the best yields compared to the other rootstocks. Production of 'Doyenne du Cornice' was the best on QA and BP3. In newly planted 'Rosemarie', 'Flamingo', and 'Forelle' trials, different rootstocks were evaluated. 'Rosemarie' showed indications 01 incompatibility with QA and QC5 I, but on BPI and QA with a 'Beurre Hardy' interstock produced good initial yields although BPI induced slightly larger trees. 'Flamingo' on QA and QC51 produced the best yields. 'Forelle' on BPI, BP3, and QA produced similar yields up to the 4th leaf. For apples M793 seems to be the preferred rootstock in the South African industry. From production records of 'Golden Delicious' and 'Granny Smith' apples, it appeared that M793 and MM 106 produced the best yields when compared to Seedling rootstock. In a 'Cripps' Pink' trial, MMI09, M793 and M25 were more vigorous than M7, MMlll and MMI06. MMI06 was cumulatively, over four years from planting, the most yield efficient, although no consistent trend regarding fruit quality was observed between the rootstocks evaluated.
AFRIKAANSE OPSOMMING: Vertraagde bot word algemeen opgemerk in appel en peer produserende lande met n' warm enlof kort winter klimaat, wat veroorsaak dat bot van minder knoppe, meer ongelyk plaasvind en gevolglik lei tot swakker opbrengste. Toediening van rus-breek middels beperk die simptome van vertraagde bot. Volgens koue modelle wat in vrugte produserende areas in die wereld gebruik word, is Suid Afrika se klimaat nie geskik vir die verbouing van sagtevrugte nie alhoewel sagtevrugte al vir n lang tyd met groot sukses hier verbou word. Hierdie studie is daarop gemik om die effek van vries temperature, verskillende koue temperature en die periode van koue op die vrystelling van dormansie op knoppe van appel en peer lote te kwantifiseer. Die peri ode van koue was die mees belangrikste faktor wat die ontwikkeling van dormansie beinvloed het. In sommige gevalle was die effek van die koue temperature betekenisvol, maar die bydrae tot die verskille in die ontwikkeling van dormansie was weglaatbaar klein. Ons bevindinge dui aan dat die huidige koue modelle wat gebruik word, meer klem moet le op die periode van blootstelling aan koue as aan die effek van verski llende temperature tussen 1 en 10°e. Die Suid Afrikaanse appel en peer bedryf het goeie vordering in die beweging na hoe digtheid aanplantings gemaak, maar groot verskille in grond tipes, sub-optimale groei toestande, herplant probleme en n' tekort aan ondervinding met dwergende onderstamme het verdere ontwikkeling beperk. Ten spyte hiervan is daar steeds n' dringendheid om hcer produksies, van goeie kwaliteit vrugte so vroeg as moontlik te kry deur die toepassing van die mees effektiewe produksie praktyke. Ons het gepoog om die prestasie van plaaslik beskikbare appel en peer onderstamme te kwantifiseer, in besonder deur data wat nog nie van tevore oor verslag gedoen is nie en ook deur te meld van vroee produksies op nuut aangeplante proewe. lnformasie uit die bedryf het aangedui dat BP 1 en BP3 die voorkeur peer onderstamme is. Deur na die produksie geskiedenis van 'Packham's Truimph' pere te kyk kom dit voor of BP3 en OHxF97 die beste produksies in vergelyking met die ander onderstamme gelewer het. Die produksie van 'Doyenne du Com ice' was die beste op QA en BP3. In nuut geplante 'Rosemarie', 'Flamingo', en 'Forelle' pro ev e was verskillende onderstammc gecvalueer. 'Rosemarie' het tekens van onverenigbaarheid getoon met QA en Q 51, maar op BP 1 en QA met n 'Beurre Hardy' tussenstam het 'Rosemarie' goeie aanvanklike opbrengste gel ewer, al was bome op BP 1 effens grater. 'Flamingo' op QA en QCS 1 het die beste opbrengste gelewer. 'Forelle' op BP 1, BP3, en QA het ongeveer ewe veel tot die 4dc blad geproduseer. By appels is M793 die voorkeur onderstam van die Suid-Afrikaanse appel bedryf. M793 en MM 106 gee beter opbrengste as Saailing onderstam wanner die produksie geskiedenis van 'Golden Delicious' en 'Granny Smith' appels evalueer word. In n' 'Cripps' Pink' proefwas MMI09, M793 en M2S meer groeikragtig as M7, MMlil en MMI06. MM I 06 het kumulatief meer geproduseer en was ook meer produsie doeltreffend tot en met die 4de blad na plant. Daar was egter geen konstante neiging rakende vrug kwaliteit tussen die verskillende onderstamme wat getoets is nie.

More than 80% of vineyards around the world use grafted plants: a scion of Vitis vinifera grafted onto a rootstock of single or interspecific hybrids of American Vitis species, resistant or partially resistant to Phylloxera (Daktulosphaira vitifoliae (Fitch, 1856)). The genetic variability of grapevine rootstocks plays a fundamental role in their adaptation to the environment (Serra et al., 2013). In the climate change scenario, predicting an increase of aridity in the near future (Dai, 2013), the more frequent and severe drought events may represent the major constrain for the future of viticulture (IPCC, 2018; Schultz, 2000). Therefore, the selection of new rootstocks able to cope with unfavourable environmental condition is a key asset, as well as a strategy to improve crop yield/vegetative growth balance on scion behaviour (Corso and Bonghi, 2014). So far, the influence of rootstock on scion physiological performance during water stress has always aroused great interest. On the contrary, the scion impact on rootstock response is still less debated. Therefore, the effect of grafting on rootstock behaviour have been investigated. Phenotypical and large-scale whole transcriptome analyses on two genotypes, a drought-susceptible (101-14) and a drought-tolerant (1103 P), own-rooted and grafted with Cabernet Sauvignon, subjected to a gradual water shortage in semi-controlled environmental conditions have been performed. The ungrafted condition affected photosynthesis and transpiration, meaning the decisive role of scion in modulation of gas exchanges and in general in plant adaptation. Molecular evidence highlighted that the scion delays the stimulus perception and rootstock reactivity to drought. Since 1985, the DiSAA research group operating at the University of Milan is carrying on a rootstock crossbreeding program which has led to the release of four genotypes: M1, M2, M3 and M4. They show from moderate to high tolerance to drought (M4 > M1 = M3 > M2). In order to characterize their performance during water stress, their physiological (gas exchanges and stem water potential) and transcriptome response (genes involved in ABA-synthesis and ABA-mediated responses to drought) under well-watered and water stress conditions were examined. The behaviour of M-rootstocks (M1, M2 and M3) was compared with that of other commercial genotypes largely used in viticulture, either tolerant (140 Ru, 41 B, 110 R, 1103 P), less tolerant (SO 4, K 5BB) and susceptible (420 A and Schwarzman). Discriminant analysis (DA) showed that when water availability starts to decrease, rootstocks firstly perceives the stress activating a transcriptome response, consequently physiological changes have been observed. It also demonstrated that the three M-rootstocks were clearly discriminated: M4 was grouped with the most tolerant genotypes while M3 with the less tolerant or susceptible ones from a physiological standpoint, confirming their different attitude to tolerate water stress. M4 has proven to be a promising rootstock due to its ability to adapt to drought conditions. Considering the constant great demand for vine planting materials, the obtainment of genetically homogeneous populations (i.e. clones) from elite individuals through micropropagation represents a rapid alternative to conventional multiplication. For this reason, an efficient high-throughput protocol for M4 in vitro propagation was set up. Its attitude to shooting, root development and callus proliferation was compared to that of other rootstocks largely used in viticulture (K5BB, 1103P, 101-14 and 3309C). Moreover, pro-embryogenic and embryogenic callus from bud explants were also produced, representing a cellular material manipulable with the genetic engineering techniques. In water scarcity condition, among the mechanisms activated by M4, the great ability to scavenge ROS, related to the increased accumulation of stilbenes and flavonoids, may be such as to give it tolerance to the stress. In particular, the higher levels of trans-resveratrol were correlated with the up-regulation of some stilbene synthase genes, mainly VvSTS16, VvSTS18, VvSTS27 and VvSTS29. The over expression of these genes was linked to a structural variation in their promoter region. To confirm that VvSTSs genes may be considered putative factors of M4 better adaptation to water stress, a genome editing protocol based on the CRISPR/Cas9 system, aimed at knock-out the genes, was performed. For testing the gRNAs functionality, a transient assay on in vitro micropropagated plantlets of M4 and 101-14 was performed. The positive results obtained by this experiment will lead to the transformation of somatic embryos and regeneration of whole-edited plants using the vectors developed.

Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
With the aim of evaluating the potential of five ‘Rocha’ pear clones (1, 2, 4c, T and Z), a field trial was conducted at APAS orchard, located in Cadaval (Sobrena). All the clones were grafted onto Provence BA-29, EMA and Sydo rootstocks and trees grown under identical soil and climate conditions. The agronomic characteristics and economic results were evaluated from 2006 until 2011 corresponding to the 5th and 10th year of the orchard. The field trial indicated that there isn’t an ideal combination of clone/rootstock leading to earlier pear production, trees with medium vigour, high fruit size, rounded shape fruits and good fruit quality. Combinations of Z/Sydo, 2/Sydo and T/Sydo reached the highest yields per unit/tree while the most productive was T/Sydo followed by Z/Sydo with a revenue of 42.968,3 €.ha-1 and 38.551,7 €.ha-1, respectively. Among the clones there were slight differences in fruit quality, the clones 4c, 1 and T produced pears with the highest fruit size and roundest shape fruits while the fruits of the clone T had the highest total solid soluble content. The rootstock Sydo achieved better results when comparing to BA-29 and EMA, resulting in earlier production of pears and increased yields, high fruit size and better shaped fruits.

A lemon rootstock trial and a lemon scion trial were established in 1993. After two years growth, C. volkameriana and C. macrophylla rootstocks have begun to show significant growth and yield increases, compared with 'Swingle' citrumelo and 'Carrizo' citrange. These two rootstocks also have led to larger fruit size, especially early in the season. Trees on Rough lemon rootstock had equivalent growth, but less yield. 'Limoneira 8A Lisbon' scion cultivar had the greatest yield and largest early season fruit size, compared to 'Frost Nucellar', 'Corona Foothills' and `Prior Lisbon' lemons.

Dans le contexte du changement climatique, les prédictions réalisées mettent en évidence une altération de la disponibilité en eau dans de nombreuses régions viticoles ; ce qui, conjointement à l’augmentation de la population mondiale et la diminution des terres agricoles, va accroître la compétition pour l’utilisation des ressources hydriques. Par conséquent, améliorer l'adaptation à la sécheresse de la vigne est un des enjeux majeurs des prochaines années. Pour cela, une adaptation des pratiques culturales peut être proposée, en particulier le choix pertinent du matériel végétal et notamment du porte-greffe.Dans ce travail, le rôle du porte-greffe vis-à-vis de la réponse de la vigne greffée à la contrainte hydrique a été étudié, en utilisant des approches écophysiologiques, moléculaires et de modélisation. Des expériences ont été réalisées en conditions contrôlées afin d’étudier l’effet du déficit hydrique à court et long terme sur les réponses de différents porte-greffes greffés avec le même scion.Le modèle écophysiologique a démontré que les porte-greffes affectent l'ouverture stomatique du greffon par des processus coordonnés incluant les caractéristiques racinaires, les signaux hydrauliques et les signaux chimiques lors d’un déficit hydrique à court terme. La conductance stomatique, le taux de transpiration et la conductance hydraulique des feuilles ont été plus élevés en conditions irriguées et de stress hydriques modérés chez le génotype résistant à la sécheresse (110 Richter) par rapport au génotype sensible à la sécheresse (Vitis riparia cv. Gloire de Montpellier). Nous avons identifié plusieurs paramètres génétiques impliqués dans le contrôle de la régulation stomatique. Des différences d’architecture racinaire et de conductivité hydraulique des racines ont été identifiées entre les porte-greffes.Le déficit hydrique à long terme a entrainé des réponses adaptatives différentes entre les porte-greffes. Le génotype tolérant la sécheresse a induit une modification du diamètre des vaisseaux du xylème de la partie apicale de la racine en réponse au déficit hydrique modéré tandis que le génotype sensible n'a pas présenté de différence par rapport au contrôle. L’analyse transcriptomique des racines a identifié des gènes spécifiques aux différents génotypes, qui sont régulés en fonction du niveau de déficit hydrique. La comparaison entre les niveaux de stress et les génotypes a identifié 24 gènes intervenant dans l’interaction « traitement × génotype ». Ces gènes sont majoritairement impliqués dans le métabolisme des lipides et de la paroi cellulaire. Des courbes de réponse au déficit hydrique spécifiques aux différents génotypes ont été observées. La protection contre les dommages liés aux stress oxydatifs induits par le stress hydrique semble être un mécanisme important chez le porte-greffe résistant à la sécheresse. Le génotype sensible semble répondre au déficit hydrique par une modification des propriétés de la paroi cellulaire de la racine
Climate change raises concerns about temporal and spatial water availability in many grape growing countries. The rapidly increasing world population and the scarcity of suitable land for agricultural food production, together with a changing climate, will increase competition with grape-producing areas for the use of land and resources. Consequently, other practices that can potentially improve water management of vineyards and water acquisition by grapevines need to be considered. Aside from canopy systems and their management, the choice of plant material is a key issue. Therefore, in the present work, the role of different rootstocks, regarding their tolerance to drought, was investigated for their potential effects on i) water uptake, ii) water transport and iii) shoot water use, using a combination of ecophysiological, modelling and transcriptomic approaches. Experiments were conducted under controlled conditions to decipher short and long term responses to drought of different rootstocks grafted with the same scion. An ecophysiological model was used to investigate the roles of rootstock genotypes in the control of stomatal aperture. Long-term steady state water-deficit conditions were used to examine the responses of i) whole plant growth, root anatomy and hydraulic properties and ii) transcriptome remodelling in the roots.Our model showed that rootstock affect stomatal aperture of the grafted scion via coordinated processes between root traits, hydraulic signals and chemical signals. Stomatal conductance, transpiration rate and leaf-specific hydraulic conductance were higher and better maintained under well-watered and moderate water-deficit conditions in the drought-tolerant genotype (110 Richter) compared to the drought-sensitive one (Vitis riparia cv. Gloire de Montpellier). We identified several genotype-specific parameters which play important roles, like root-related parameters, in the control of stomatal regulation. Additionally, root system architecture and root hydraulic properties are important constitutive traits identified between rootstocks.Long-term water-deficit induced genotype adaptive responses in the roots were evaluated. The drought-tolerant genotype exhibited a substantial shift in root tips xylem conduit diameter under moderate water-deficit while the drought-sensitive genotype did not respond. Transcriptomic analysis identified genotype-specific transcripts that are regulated by water-deficit levels. The comparison between stress levels and genotypes identified 24 significant genes in “treatment×genotype” interactions, most of them were involved in lipid metabolism and cell wall processes. These genes displayed genotype-specific water-deficit response curves. Protection against drought-induced oxidative damage was found to be an important mechanisms induced by the drought-tolerant rootstock, while the drought-sensitive one responds to water-deficit by modification of cell wall properties

Au cours des prochaines années, il est prédit que le changement climatique va influencer la production des cultures et impacter négativement le secteur agricole. Parmi les cultures mondiales majeures, la vigne est cultivée pour ses baies, qui sont la base de produits à forte valeur ajoutée (vin, liqueurs, et métabolites secondaires utilisés dans les industries pharmaceutiques et cosmétologiques) et dont le métabolisme est fortement sensible au climat. Cependant, la réponse au niveau de la composition de la baie ainsi que les mécanismes moléculaires sous-jacents mettant en évidence la capacité de l’interaction porte-greffe/greffon à influencer le métabolisme de la baie dans des conditions de stress hydrique est encore très peu étudiée et compris. Dans ce contexte, cette thèse a eu pour objectifs de répondre à ces questionnements en combinant des approches ecophysiologique, biochimique et transcriptomique. Vitis vinifera cv. Pinot noir greffé soit sur le porte greffe 110R (tolérance à la sécheresse, vigueur conférée moyenne à forte) ou 125AA (sensible à la sécheresse, forte vigueur conférée) ont été étudié au cours de 3 années (2009, 2010 et 2011), au vignoble et dans des conditions témoin (pluviométrie normale) ou de stress hydrique provoqué. Différents paramètres physiologiques (statut hydrique et rendement) ainsi que le profil métabolique de la baie (sucres, acides organiques, acides aminés et anthocyanes) ont été caractérisés à quatre stades de développement (E-L 33, E-L 35, E-L 36, E-L 38). D’autre part, une analyse microarray sur génome complet a également été réalisée pour deux années (2009 et 2010) et deux stades de développement critiques et représentatifs (E-L 35 et E-L 36). Dans son ensemble, cette thèse fournit des nouveaux éléments concernant la réponse métabolique de la baie au porte-greffe et à la contrainte hydrique, et met en évidence des possibles mécanismes moléculaires impliqués dans cette réponse
Climate change is expected to influence crop production and to impact negatively the agricultural sector in the future. Among the major crops cultivated worldwide, grapevine provides berries that are the basis of high added value products (wines, liquors, and secondary metabolites used in the pharmaceutical and cosmetological industry) and whose metabolism is strongly sensitive to climate (vintage effect). However, the response of berry composition and the molecular mechanisms underlying the ability of rootstock/scion interaction to influence grape berry metabolism under drought stress are still poorly understood. In this context, this work aimed to fill the gaps on the aforementioned questions by combining comprehensive ecophysiological measurements, detailed metabolite analysis, and whole-genome transcriptome analysis. Vitis vinifera cv. Pinot noir grafted on either rootstock 110R (drought tolerant, mid- to high vigor) or 125AA (drought sensitive, high vigor) were studied during three growing seasons (2009, 2010, and 2011) in the field under normal rainfall or water shortage conditions. We characterized different physiological parameters (water status and yield components) and berry metabolomic profiles (sugars, organic acids, free amino acids and anthocyanins) during four developmental stages (E-L 33, E-L 35, E-L 36, E-L 38). Besides we also performed a microarray analysis in two years (2009 and 2010) at two critical and representative developmental stages (E-L 35 and E-L 36). Overall, this work provides novel insights into the response of grape berry metabolites to rootstock and to drought and uncovers some possible molecular mechanisms underlying the berry response to different rootstock/water status combinations

Four 'Lisbon' lemon selections from the University of Arizona Citrus Budwood Certification plot were selected for evaluation on Citrus rootstock 'Frost Nucellar', 'Corona Foothills', 'Limoneira 8A' and 'Prior' were selected because of their popularity among Arizona growers or because of the lack of information about their performance under Arizona climactic and edaphic conditions. Trees were planted in 1993. Early results indicate that the 'Limoneira 8A Lisbon' selection is outperforming the other selections in both growth and yield. In a similar trial, five rootstocks were selected for evaluation using 'Limoneira 8A Lisbon' as the scion. Carrizo citrange, Citrus macrophylla, Rough lemon, Swingle citrumelo and Citrus volkameriana were chosen. Trees were planted in 1993. Early results indicate that trees on C. volkameriana are superior to those on other rootstocks in both growth and yield.

Five rootstocks, 'Carrizo' citrange, Citrus macrophylla, Rough lemon, Swingle citrumelo and Citrus volkameriana were selected for evaluation using 'Limoneira 8A Lisbon' as the scion. Early results indicate that trees on C. volkameriana and C. macrophylla are superior to those on other rootstocks in both growth and yield. 'Swingle' and Carrizo' are performing poorly. In a similar trial, Four 'Lisbon' lemon selections, 'Frost Nucellar', 'Corona Foothills', 'Limoneira 8A' and 'Prior' from the University of Arizona Citrus Budwood Certification plot were selected for evaluation on Citrus volkameriana rootstock. Early results indicate that the 'Limoneira 8A Lisbon' selection is outperforming the other selections in both growth and yield. Preliminary results from another lemon cultivar trial and a navel orange cultivar trial are presented as well.

Five rootstocks, 'Carrizo' citrange, Citrus macrophylla, Rough lemon, Swingle citrumelo and Citrus volkameriana were selected for evaluation using 'Limoneira 8A Lisbon' as the scion. 1998-99 results indicate that trees on C. macrophylla and C. volkameriana are superior to those on other rootstocks in both growth and yield. C. macrophylla is beginning to outperform C. volkameriana. 'Swingle’ and Carrizo' are performing poorly. In a similar trial, Four 'Lisbon' lemon selections, 'Frost Nucellar', 'Corona Foothills', 'Limoneira 8A' and 'Prior' were selected for evaluation on Citrus volkameriana rootstock. 1998-99 results indicate that the 'Limoneira 8A Lisbon' selection is superior, and that ‘Corona Foothills Lisbon’ may also be superior to the other selections in both growth and yield. Results from another lemon cultivar trial suggest that 'Cavers Lisbon', 'Limonero Fino 49' and 'Villafranca’ lemons may be good candidates for plantings as well. Results from two other lemon scion trials, a navel orange cultivar trial and a 'Valencia' orange trial are presented as well.

Five rootstocks, 'Carrizo' citrange, Citrus macrophylla, Rough lemon, Swingle citrumelo and Citrus volkameriana were selected for evaluation using 'Limoneira 8A Lisbon' as the scion. 1999-2000 results indicate that trees on C. macrophylla and C. volkameriana are superior to those on other rootstocks in both growth and yield. C. macrophylla is outperforming C. volkameriana. Rough lemon is intermediate, and 'Swingle' and Carrizo’ are performing poorly. In a similar trial, Four 'Lisbon' lemon selections, 'Frost Nucellar', 'Corona Foothills', 'Limoneira 8A' and 'Prior' were selected for evaluation on Citrus volkameriana rootstock. 1998-99 results indicate that the 'Limoneira 8A Lisbon' and 'Corona Foothills Lisbon' are superior in yield and fruit earliness. Results from another lemon cultivar trial suggest that 'Cavers Lisbon', 'Limonero Fino 49' and 'Villafranca' lemons may be good candidates for plantings as well. Results from two other lemon scion trials, a navel orange cultivar trial and a 'Valencia' orange trial, and a mandarin trial are presented as well.

Five rootstocks, 'Carrizo' citrange, Citrus macrophylla, Rough lemon, Swingle citrumelo and Citrus volkameriana were selected for evaluation using 'Limoneira 8A Lisbon' as the scion. 1994-2000 results indicate that trees on C. macrophylla and C. volkameriana are superior to those on other rootstocks in both growth and yield. C. macrophylla is outperforming C. volkameriana. Rough lemon is intermediate, and 'Swingle' and Carrizo’ are performing poorly. For 2000-01, rough lemon trees performed similarly to C. macrophylla and C. volkameriana. In a similar trial, Four 'Lisbon' lemon selections, 'Frost Nucellar', 'Corona Foothills', 'Limoneira 8A' and 'Prior' were selected for evaluation on Citrus volkameriana rootstock. 1994-2001 results indicate that the 'Limoneira 8A Lisbon' and 'Corona Foothills Lisbon' are superior in yield and fruit earliness. Results from another lemon cultivar trial suggest that 'Cavers Lisbon', 'Limonero Fino 49' and 'Villafranca' lemons may be good candidates for plantings as well. Results from three other lemon scion trials, a navel orange cultivar trial and a 'Valencia' orange trial, and a 'Fallglo' mandarin trial are presented as well.

Grapevine leafroll disease (GLD) is recognised as a global, economically significant viral disease of grapevines. The complex of viruses associated with GLD are termed the Grapevine leafroll associated viruses (GLRaVs) and are sequentially numbered in order of discovery. The main etiological agent of GLD is GLRaV-3. In South Africa, a grapevine certification scheme exists to ensure grape growers have access to leafroll-free planting material. Unlike red Vitis vinifera cultivars, rootstocks infected with GLD are asymptomatic and cannot be identified through visual diagnosis. To complicate matters even further, GLRaV-3 is thought to be poorly detected in rootstock tissue due to uneven distribution of virus and erratic viral titres. Difficulties in GLRaV-3 detection could result in asymptomatic carriers of GLD being grafted onto healthy vines. To assess the supposed poor detection of GLRaV-3 in rootstocks, a survey was conducted in the Western Cape, South Africa, where various rootstock types and corresponding scions were sampled from 60 vines located in former mother blocks. Samples were tested using a reverse transcription-PCR directed against the conserved GLRaV-3 helicase gene. Detection of GLRaV-3 was significantly higher in scion (83%) than in corresponding rootstock tissue (15%). Several graft transmission experiments were conducted to gain a better understanding of the nature of the poor detection of GLRaV-3 in infected rootstocks. Detection of GLRaV-3 varied depending on the rootstock cultivar used, with Richter 110, Paulsen, and Salt Creek/Ramsey rootstocks appearing to be the least resistant to GLRaV-3 infection and subsequent replication. The variable GLRaV-3 detection in 101-14 Mgt and Richter 99 were suggested to be the result of genetic heterogeneity amongst rootstock clones. The possibility of passive movement of virus in phloem tissue cannot be ruled out and further studies will need to be done to understand this phenomenon. The optimal time of sampling and tissue region of rootstocks most suited for GLRaV-3 detection was investigated using real-time reverse transcription-PCR. Detection of GLRaV-3 was found to be significantly higher in basal tissue than in apical tissue of rootstocks Salt Creek/Ramsey and Richter 110. Rootstock US 8-7 was used as a representative control for V. vinifera and, with the exception of R110, was observed to have a significantly higher detection rate over any other rootstock cultivar. The most suitable time of sampling was determined to be late summer/beginning of autumn. A colourimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay, aided by a crude RNA extraction protocol, was adapted from Walsh and Pietersen (2013) and optimised for possible on-site GLRaV-3 detection in rootstocks. The assay was shown to be rapid (70 minutes), specific, more sensitive than conventional reverse transcription-PCR, and holds promise for detection of the virus in rootstocks. This comprehensive study on GLRaV-3 in rootstocks in South Africa will help bridge the gap in knowledge on the largely unknown GLD dynamics seen in rootstocks.
Dissertation (MSc)--University of Pretoria, 2019.
Microbiology and Plant Pathology
MSc
Unrestricted

In many areas, over half of all diverted water is used for irrigation. Tree fruit crops use a lot of water, but water productivity can be increased using properly-timed precision water stress. In addition to water conservation, increases in water productivity arise from better fruit quality, increased storage life and reductions in pruning and maintenance. One major hurdle to applying precision water stress in orchards is the lack of a reliable, automated method of determining tree water status. However, the influence of physiological characteristics such as rootstock vigor on water productivity are also important. Selecting the most appropriate rootstocks and accurately determining the water status of orchard trees can increase water productivity. Research has shown that some rootstocks can more effectively extract water from soil. In this research, the response to water stress of three different Gisela tart cherry dwarfing rootstocks was compared using a weighing lysimeter system. Gisela 12 and Gisela 3 rootstocks recovered from drought stress more quickly and had higher trunk diameter growth rates than drought-stressed Gisela 5 rootstocks. Two potential methods of determining tree water status were also evaluated. Trunk hydration was measured using electromagnetic sensors and canopy temperature changes were detected using infrared radiometry. Electromagnetic techniques, including time domain reflectometry, can be used to determine the water content of wood. Until recently, the cost of this technology has inhibited its widespread use, but new affordable commercial electromagnetic soil moisture sensors have created renewed interest in this technique. In this research five different types of electromagnetic soil moisture sensors were inserted into the trunks of fruit trees and were monitored over two growing seasons. Maximizing exposure of waveguides to the sapwood increased the response of these sensors to changes in stem water potential. Infrared measurements of canopy temperature have successfully been used with field crops. However, the heterogeneity of orchard canopies makes this technique more difficult in orchards. Here, the efficacy of aiming radiometers at single trees versus at entire orchards was compared over multiple growing seasons. Neither single tree measurements nor whole orchard techniques produced a sufficiently robust signal to recommend them for general use.

Gisela 5 consists one of the most promising new cherry rootstocks in Greece. However, there is a gap in the knowledge related to the propagation of this rootstock by using cuttings. The first aim of this study was to investigate different methods to propagate the cherry rootstocks Gisela 5. The results showed that, this rootstock can be propagated by using hardwood cuttings with a moderate percentage of rooting (about 60%). More investigation is required in testing the frequency of irrigations on the rooting of cuttings in different rooting media. In addition, ·more work should be done to examine the possibly effect of endogenous hormones, carbohydrates and mineral elements in rooting of cuttings. It could help to understand better how the external factors affect the percentage of rooting of Gisela 5 cuttings. Propagation of Gisela 5 by using semi-hardwood cuttings is also possible, but the percentage of rooting is relatively low (about 35%). Improvement of the rooting could be achieved if the effect of some other external factors, such as etiolating, age of mother trees etc is examined. In cases of which propagation of Gisela 5 cherry rootstock with hardwood and semi-hardwood cuttings is impossible, the use of root cuttings could be another way to propagate this rootstock with high percentage of sprouting (67%) and rooting (90%) of achievement. More investigations should be conducted to evaluate the effect of higher concentrations of BOP on improvement of sprouting. This study also gives data for the use of micrografting as a quick grafting method. The percentage of achievement of micro grafted plants can be improved in a percentage of 55-60% if parafilm is used as wrapping material and covering of plants after micrografting. A method to produce inter-stocked trees with the micrografting method was evaluated although the percentage of achievement is low (10%). In this study, using micrografting in combination to thermotherapy was not an effective method to produce virus-free, probably because of inappropriate method of thermotherapy. More investigation should be done by using different method of thermotherapy so that vims-free plants will be able produced. In this study, experiments were conducted to i.mprove the tissue culture propagation of Gisela 5 , cherry rootstock additionally to the previous works. The results showed that shoot apices possibly are appropriate explants for the tissue culture propagation of this rootstock. Adding 5 ~lM lBA in combination to 0.15 glucose in medium improved the rooting of Gisela 5 explants. Supplement of medium with BA at concentrations as low as 0.5 ~M in'creased the sprouting of explants. Considering the importance of the diseases Phytophthora crown rot, Crown gall and Bacterial canker for the cultivation of cherry trees, it is essential for growers to know the level of susceptibility of Gisela 5 to these diseases. In this study, the susceptibility of cherry rootstock Gisela 5 to infection by Phytophthora Crown Rot (P. cactorum, P. citrophthora, P. parasitica, and P. citricola), Crown Gall (Agrobacterillm tllmejaciens) and Bacterial Canker (Pselldonwnas syringae) was evaluated. The results showed that Gisela 5 is susceptible to all pathogens tested. The susceptibility of Gisela 5 in natural infections by the pathogens used in this study should also be evaluated. Before that, caution should be taken when Gisela 5 is used in locations where these diseases are endemic and this may limit its commercial potential.

The influence of 12 different rootstocks on yield and quality of "Redblush" grapefruit was studied for several years. Rootstocks consisted of: macrophylla, volkameriana, rough lemon, Palestine sweet lime, sour orange, Carrizo citrange, taiwanica, Savage citrange, Citrumelo, Ichang pummelo, Troyer citrange and Cleopatra mandarin. Trees on volkameriana, Palestine sweet lime, rough lemon, and sour orange had higher yield than other rootstocks, while trees on Savage citrange had lowest yield. However, soluble solids and acid /sugar ratio were relatively low in the fruits on volkameriana but high in fruit on Savage citrange rootstocks.

The influence of rootstock and canopy density on grape berry composition was investigated over the summer of 2003-2004 on a commercial vineyard at Waipara, North Canterbury. This experiment was designed to investigate the influence of rootstock and canopy density on the acid composition, potassium (K) content and final pH of harvested fruit (Pinot Noir AM 10/5 Lincoln Selection). The trial block consisted of eight rootstocks laid out to an 8 x 8 latin square, each plot consisting of five vines of the same rootstock. Two canopy treatments were overlaid the block (down whole rows, assigned randomly, four rows to each treatment); one treatment allowed to grow naturally, in the other treatment the canopy was thinned removing double burst shoots and laterals. The bunch numbers were adjusted in the Unthinned canopy treatment (UCT) to match the Thinned canopy treatment (TCT). Information was gathered to assess: the canopy size and density (Pinot Quadrat Leaf Layer and Percent Gaps and canopy porosity), the plant yield (and berry size, berries per cluster, cluster weight, clusters per plant), plant K levels at flowering and veraison (from petioles and leaf blades) and berry composition at harvest (soluble solids (as brix), K, titratable acidity (TA), tartaric acid concentration, malic acid concentration and pH). The trial area was non-irrigated on clay loam soils and viticultural management was to best commercial practice. It was found that although rootstock influenced the levels of K in the plant and in the juice at harvest, the level of K in the juice did not influence pH in this experiment (range of rootstock juice K: 808 ppm to 928 ppm, l.s.d. = 75 ppm). The level of tartaric acid concentration in the juice was found to be the dominant influence on the level of pH in this experiment (rootstock pH range: 3.21 to 3.39, l.s.d. = 0.05). The juice concentration of tartaric acid was influenced by both rootstock (rootstock range 4.0 to 4.7 g/L, l.s.d = 0.4) and canopy density (UCT = 4.1, TCT = 4.7, l.s.d. = 0.4), decreased shading positively increasing the level of tartaric acid. The malic acid concentration in the juice was positively influenced by increasing canopy density (UCT = 4.7 g/L, TCT = 4.1 g/L, l.s.d = 0.4) and this played a minor role in the determination of pH in this experiment; an influence of rootstock on the level of malic acid concentration was found. The malic acid concentration strongly influenced the determination of TA (UCT = 11.0 g/L, TCT = 10.2 g/L, l.s.d = 0.5); tartaric acid concentration had a minor influence on the recorded TA. Attempts to characterise the influence of rootstock on malic acid, tartaric acid and pH were inconclusive. Rootstock was found to influence the canopy variables measured in this experiment and the recorded average plant yield. Crosses of Vitis rupestris were found to exhibit the most canopy vigour and those derived from Vitis berlandieri and Vitis riparia the least. The Canopy treatment did not show an influence over yield but the rootstock was found to influence plant yield, through the numbers of berries set in a cluster and the final harvest cluster weight. The influence of rootstock on pH may be described by the influence it exerts on canopy growth and yield but this was thought unlikely. Further research is required to describe the nature of the rootstock influence on K, malic acid, tartaric acid and pH.

Mestrado Vinifera EuroMaster - Instituto Superior de Agronomia
Despite the expansion and increasing popularity of Touriga Nacional in Portugal, there has not been extensive research into the variety’s response to different climate characteristics. The objective of this research was to test the relationships between different climate indices and berry composition and Phenology in Touriga Nacional in the Dão winegrowing region of Portugal. From the available data we can also explore any evolution of the climate, berry composition and phenology during the recorded years. Climate and plant data were analysed from records collected at the ‘Centro de Estudos Vitivinícolas do Dão’ in the Dão wine region of northern Portugal between 1963-2010. Six common climate indices were calculated from the climate data for each year of the recorded period. Berry composition and phenology records of Touriga Nacional planted at the research centre from the same period were also analysed. Any trends or changes in climate or plant data were analysed over the recorded period. All climate indices were correlated against the plant data results to investigate any relationships. An increase in the Growing Season Temperature Index (GST) during the studied period was observed that agreed with studies over a similar period in other parts of Europe. Various climate indices had some correlations with grape and vine parameters; generally acidity showed a reduction with increases in temperature-based indices. Budburst and harvest dates were confirmed to be hastened by higher temperatures. Also yield had a positive correlation with the Huglin Index (HI). HI had a better correlation with plant parameters because it is calculated over a 6-month (instead of 7-month) period. Understanding the relationship between Touriga Nacional and climate indices can help producers maximise the potential of this important Portuguese variety, as well as aid its expansion into other Portuguese regions and other regions throughout the world

Wine vineyards in humid environments like the mid-Atlantic United States are characterized by vines that develop too much vegetative growth for optimum quality wine production. Cover crops, rootstocks and rootzone restriction were evaluated for their effect on vegetative and reproductive growth on Cabernet Sauvignon. Treatments were arranged in a strip-split-split plot arrangement with under-trellis cover crops (UTCC) compared to row-middle only cover crop combined with 1-m weed-free strips in the vine row as main plots. Rootstocks riparia Gloire, 420-A, and 101-14 were sub-plots, while sub-sub-plots comprised two treatments: vines were either planted in root-restrictive (RR), fabric bags (0.016 m3) at vineyard establishment (2006), or were planted without root restriction. All three factors were effective in suppressing vegetative development as measured by rate and extent of shoot growth, lateral shoot development, trunk circumference, and dormant pruning weights. Canopies of vines with UTCC and RR had reduced leaf layer values by approximately 21% and 23% compared to conventional controls. The principal effect of the UTCC and the RR treatments was a sustained reduction in stem (xylem) water potential. UTCC and RR caused significant 7 and 10% reductions in berry weight, compared to their conventional controls. Berry weights of vines grafted to riparia were greater than those of vines grafted to other rootstocks. Wine made from UTCC and RR treatments increased red wine color compared to herbicide UTGC and NRR, respectively. This study identified treatments that improve vine balance while simultaneously improving grape composition and potential wine quality.
Master of Science

Vineyards in the Mid-Atlantic often have large, vigorous vines that can be costly to manage and produce inadequate fruit for wine production. Dense canopies increase the incidence of fungal disease, require greater allocation of resources to manage, and inhibit fruit development. The primary objective of these studies was to determine effective vine-size modification treatments that would optimize fruit quality, while reducing labor and chemical control. Research factors included root manipulation, under-trellis ground cover, and rootstock. Treatment levels were root bag (RBG) or no root manipulation (NRM); under-trellis cover crop (CC) or herbicide strip (HERB); and one of three rootstocks: 101-14, Riparia Gloire, or 420-A. Effects of these treatments were measured in two experiments: Experiment I compared combinations of all three treatments, while Experiment II explored the individual effects of root restriction using root bags of varying volumes. Root restriction consistently demonstrated the ability to reduce vegetative growth and vine water status. In the first experiment fruit-zone photosynthetic photon flux density (PPFD) was increased by 234% in RBG vines. Timed canopy management tasks indicated that RBG canopies required about half the labor time of NRM canopies. Anthocyanin concentration and total phenolic content were increased by 20% and 19% respectively in RBG fruit. CC increased fruit-zone PPFD by 62%, and increased soluble solids and color compounds. The 420-A rootstock reduced potassium uptake, resulting in lower must potassium concentration. Results demonstrated that these treatments significantly reduce vegetative growth in a humid climate, decrease management labor, and produce higher quality fruit.
Master of Science

Methyl bromide fumigation, a common method used to combat soil borne pathogens in commercial tomato cultivation, was to be limited by the Montreal Protocol due to concerns of ozone depletion. Alternative methods to protect tomatoes against diseases include grafting. However, short grafted transplants may expose the scion to the soil. To avoid scion exposure, hypocotyl elongation of two tomato rootstocks 'Maxifort' and 'Aloha' via end-of-day far-red (EOD-FR) was examined in terms of light quality (red to far-red ratio, or R/FR) and dose (product of far-red intensity and duration). In EOD-FR light quality experiments, 'Aloha' seedlings were exposed to unfiltered and filtered incandescent light with an R/FR of 0.5 and 0.05 respectively. The resulting hypocotyl elongation was higher in filtered light than either the unfiltered light or the untreated control. Hypocotyl elongation response to EOD-FR dose in 'Aloha' and 'Maxifort' was affected by both far-red intensity and treatment duration. A saturating response was also found within a far-red dose between 0–8 mmol•m⁻²•d⁻¹ and modeled using non-linear regression with a three parameter Michaelis-Menten equation to estimate the far-red dose required to obtain near-maximum hypocotyl elongation for 'Aloha' and 'Maxifort'. The far-red dose required was affected by cultivar and experimental period. None of the EOD-FR treatments affected plant mass or stem diameter. To conclude, for maximum hypocotyl elongation using EOD-FR, the lower R/FR would increase the effectiveness of the treatment. The far-red dose should preferably be at 4–8 mmol• m⁻²•d⁻¹.

Orientador: Marco Antonio Tecchio
Banca: Sarita Leonel
Banca: Mara Fernandes Moura
Resumo: Objetivou-se avaliar a influência dos sistemas de condução do cordão principal e de porta-enxertos no desenvolvimento vegetativo, na fertilidade de gemas, na produção, nas características físico-químicas dos cachos e bagas e na evolução da maturação da uva 'Niagara Rosada'. O experimento foi realizado nos ciclos produtivos de 2014 e 2015 no município de São Manuel-SP (22° 44' S e 48° 34' O e 740 m). As plantas foram sustentadas em espaldeira baixa no espaçamento de 2,0 x 0,80 m. O delineamento experimental foi em blocos casualizados em esquema fatorial 2 x 2, correspondendo aos dois porta-enxertos ('IAC 766' e 'IAC 572') e dois sistemas de condução do cordão principal (unilateral e bilateral). Foram avaliados o número de gemas do cordão principal e o número de ramos por planta, fertilidade de gemas, comprimento, diâmetro e massa fresca dos mesmos. Durante o ciclo foram avaliadas a duração dos estádios fenológicos e a evolução da maturação das uvas. Na colheita, avaliaram-se: número de cachos por planta, produção; produtividade; massa fresca, comprimento e largura de cachos, bagas e engaços; número de bagas por cacho e relação entre comprimento e largura da baga. O mosto da uva foi avaliado quanto ao teor de sólidos solúveis (SS), acidez titulável (AT), relação SS/AT, pH e açúcares redutores. De modo geral, o sistema de condução recomendado para produção de uvas com qualidade comercial para a 'Niagara Rosada' consiste no cordão bilateral com o porta-enxerto 'IAC 766' sustentad... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The aim of this study was to evaluate the influence of training systems main cord and rootstock 'IAC 766' and 'IAC 572' in the vegetative development, bud fertility, production and the physicochemical characteristics of bunches and berries of grape 'Niagara Rosada'. The experiment was conducted in the productive cycles of 2014 and 2015 at the Experimental Farm of the Faculty of Agricultural Sciences/UNESP, located in São Manuel, SP (22º 44' S and 48º 34' W and 740 m). The plants were spaced 2,0 x 0,80 m, in espalier support system low. The treatments consisted of the combination of two rootstocks ('IAC 766' and 'IAC 572') and two training systems of the main cord (unilateral and bilateral). Were avaluated the bud number of the main cord and the number of branches per plant, the fertility evaluation of bud, length, diameter and fresh weight thereof. During the cycle were evaluated, the duration of phenological stages and the evolution of maturation the grapes. At harvest, were evaluated: number of bunches per plant; production; productivity; fresh weight, length and width of bunches, berries and stems; number of berries per cluster and the relationship between length and width of berry. The must grape was evaluated for soluble solids (SS), titratable acidity (AT), relationship SS/AT, pH and reducing sugars. In general, the recommended conduction system for commercial quality grapes for 'Niagara Rosada' consists of the bilateral cord with the 'IAC 766' rootstock, which is supported by a low espalier in the central-western region of the State of São Paulo ...
Mestre

Three orange cultivar trials have been established in Arizona, one at the Yuma Mesa Agricultural Center, Yuma, AZ and one at the Citrus Agriculture Center, Waddell, AZ. For the navel orange trial in Yuma, ‘Fisher’ navel continues to have the greatest yield, but is unacceptably granulated For the Waddell trial, the second year data has been collected, and suggests that ‘Fisher’ and ‘Beck- Earli’ are outperforming the other cultivars tested to date.

Three lemon cultivar selection trials are being conducted at the Yuma Mesa Agriculture Center in Somerton, AZ. Data from these trials suggest that ‘Limonero Fino 49’ selections may be a suitable alternative for the varieties most commonly planted in Southwest Arizona today. ‘Cavers Lisbon’ and ‘Villafranca’ might also be planted on an experimental basis

Four 'Lisbon' lemon selections, 'Frost Nucellar', 'Corona Foothills', 'Limoneira 8A' and 'Prior' were selected for evaluation on Citrus volkameriana rootstock. 1994-2002 results indicate that the 'Limoneira 8A Lisbon' and ‘Corona Foothills Lisbon’ are superior in yield and fruit size. Results for 2002-03 indicate that these cultivars as well as ‘Frost Nucellar’ have superior yield.

The apple rootstock ‘Geneva® 41’ (‘G.41’), is desirable for its resistance to fire blight, Erwinia amylovora, while producing high yields. However, ‘G.41’ and other Geneva® selections tend to form weak graft unions in the nursery that are susceptible to breaking in the wind, at harvest, or during shipment. In order to understand and remedy this graft union weakness, six scions (‘Fuji’, ‘Gala’, ‘Honeycrisp’, ‘McIntosh’, ‘Pink Lady’, and ‘Scilate’) by seven rootstocks (‘G.41’, ‘G.935’, ‘G.214’, ‘G.11’, ‘M.9-NIC 29’, ‘B.9’ and ‘EMLA 26’), by two grafting methods (chip bud and saddle graft) in a factorial design were tested for graft union flexural strength and flexibility. Additionally, plant growth regulators (PGR) were applied to trees with ‘G.41’ as rootstock as a solution to overcome graft union weakness. Transpiration rate and hydraulic resistance were measured for comparison of a weak graft forming rootstock (‘G.41’) to a strong graft forming rootstock (‘M.9-NIC 29’). ‘G.41’ consistently formed a weaker graft union regardless of scion or grafting method. Scions such as ‘McIntosh’ and ‘Pink Lady’ formed stronger graft unions, while ‘Scilate’ and ‘Honeycrisp’ form weaker graft unions. Saddle grafting did not improve graft union strength. Nurseries that graft on ‘G.41’ should choose scions that form stronger graft unions. Benzyl adenine (BA) in a latex paint increased the strength and flexibility of the graft union relative to scion cross sectional area (SCSA), but further research is needs to identify more efficient methods of application. Rootstock effect on transpiration rate was different between years. No detectable differences were found for hydraulic resistance through the graft union of different rootstocks, suggesting weaker graft unions did not limit hydraulic conductance or transpiration rate. Thus graft union weakness is not an indicator of poor vascular connection.

In apple (Malus domestica Borkh.) orchards, rootstock genotype, and soil fertility management practices impact soil fertility, plant associated soil microbial communities, and orchard productivity. Apple growers select rootstocks to confer beneficial traits, including size control, precocity, and pest and disease resistance. Rootstock genotype may also influence microbial communities, resulting in changes that affect tree health and productivity. Many apple growers apply synthetic nitrogen fertilizers to improve fruit yield and quality. In excess of tree requirements, nitrogen fertilizers may reduce crop yield and quality, as well as contribute to water pollution. The addition of carbon-based amendments, such as yardwaste, chicken litter composts, and biochar, may potentially reduce nitrogen and water loss, while improving soil structure and mineral nutrient availability. Orchard and pot-in-pot experiments were designed to study the following objectives: 1) determine the effects of integrated carbon-based fertilizer amendments on tree growth, productivity, and orchard soil fertility, 2) assess the effects of biochar on tree growth, leaf mineral nutrition, soil physiochemistry, and microbial community structure and activity, and 3) understand how rootstocks and fertilizers alter soil microbial communities. Applications of composts, integrated compost-calcium nitrate fertilizers, and biochar increased soil carbon, organic matter, cation exchange capacity and microbial respiration. In the orchard study, nitrogen fertilizer application did not increase tree growth, fruit quality, or leaf nitrogen concentration. Biochar applied at high rates with nitrogen fertigation increased tree growth and leaf nitrogen concentration similar to nitrogen fertigation. In the pot-in-pot compost study, chicken litter compost increased tree growth, and integrated compost-calcium nitrate fertilizer applications increased leaf N concentration. Analysis of the microbial community structure of bulk soil samples from the biochar and compost pot-in-pot experiments determined that the community structure was similar for all treatments during the three-year study. Metagenomic sequencing of the rhizosphere bacterial community indicated that compost applications altered community diversity and evenness, and that compost treatments were more similar to each other than to the calcium nitrate treatment. Data from my dissertation research suggests that compost can be used to increase orchard soil fertility, tree growth, and leaf nutrition, and that compost applications increase soil microbial community diversity and activity.
Ph. D.

[ES] La citricultura se enfrenta a escenarios ambientales cambiantes que provocan diferentes estreses bióticos y abióticos que pueden dificultar la producción o afectar a la calidad de la fruta. El patrón sobre el cual se injerta una variedad específica es una importante herramienta para mejorar su adaptabilidad a cada área de cultivo. En la presente Tesis se realiza el estudio del efecto del patrón sobre la calidad físico-química y nutricional de la fruta en variedades de gran interés comercial en la actualidad, mandarinas 'Clemenules' y 'Tango' y naranjas sanguinas 'Tarocco Rosso' y 'Moro'. En 'Clemenules' se llevó a cabo la evaluación de la calidad de la fruta de árboles injertados sobre ocho patrones en tres momentos de cosecha durante dos campañas. Los patrones Forner-Alcaide 13 y C-35 destacaron por adelantar el cambio de color, lo que es de gran interés comercial. Por otra parte, Forner-Alcaide V17 destacó por mantener niveles óptimos de acidez hasta el final de la campaña y presentar el mayor contenido en vitamina C, flavonoides, glucosa y fructosa. Carrizo Citrange también indujo altas concentraciones de sacarosa y vitamina C. 'Tango' es una mandarina de reciente introducción en el área mediterránea de gran interés por su periodo de recolección tardío. En esta Tesis se abordó el estudio de los cambios en la calidad fisicoquímica, nutricional y sensorial de la mandarina 'Tango' injertada sobre dos patrones (Carrizo Citrange y Forner-Alcaide 5) en las dos áreas principales de producción de Andalucía. Los resultados revelaron que la calidad de la fruta se vio influenciada por la localización de las parcelas, lo que se relacionó con la composición de la textura del suelo. En ambas localizaciones, Forner-Alcaide 5 indujo mayor contenido en acidez, sólidos solubles totales, sacarosa, vitamina C y ácido cítrico en la fruta. Las determinaciones físico-químicas, junto con la evaluación sensorial permitieron establecer el momento óptimo de recolección dependiendo de las diferentes condiciones estudiadas. También se ha incluido un estudio del comportamiento postcosecha de esta variedad, ya que no existían datos en nuestras condiciones de cultivo. La mandarina 'Tango' presentó síntomas externos de daños por frío a partir de los 20 días almacenada a 1°C y 5°C. Se realizó un estudio micro-estructural para caracterizar la alteración. Los frutos del patrón Forner-Alcaide 5 presentaron una menor incidencia de los daños por frío. El almacenamiento a 9°C no comprometió la calidad externa o interna. Dentro del grupo de naranjas, en los últimos años existe una creciente demanda por las naranjas sanguinas para su consumo en fresco. Para evaluar el efecto del patrón se tomaron las variedades 'Moro' y 'Tarocco Rosso' injertadas sobre ocho patrones. La calidad interna se vio afectada por el momento de cosecha, siendo más evidente en 'Moro'. En ambas variedades el patrón afectó a los parámetros de calidad durante la maduración. En 'Moro', se observó una reducción en el color del zumo debido a la degradación de los antocianos, siendo los patrones C-35, Macrophylla y Volkameriana los que mostraron un mayor descenso. Esta degradación se relacionó con los cambio de la temperatura durante el periodo de recolección. En 'Tarocco Rosso' no se evidenció ninguna degradación, lo que sugiere que esta variedad es menos sensible a los cambios de temperatura. En esta variedad Forner-Alcaide 5 y Forner-Alcaide 13 produjeron fruta con mayor contenido en antocianos y azúcares. Además se llevó a cabo el estudio de la aptitud a la frigoconservación de las variedades de sanguinas 'Tarocco Rosso' y 'Sanguinelli'. Mientras que la calidad interna no se vio afectada por el almacenamiento (1°C, 5°C y 9°C) durante 45 días, la fruta presentó síntomas de daños por frío a 1°C. 'Sanguinelli' presentó mayor incidencia que 'Tarocco Rosso'. Estas sanguinas se pueden almacenar entre 5 y 9°C
[CA] La citricultura s'enfronta constantment a escenaris ambientals canviants que provoquen diferents estressos biòtics i abiòtics. El patró sobre el qual s'empelta una varietat específica és una important eina per a millorar la seua adaptabilitat agronòmica en cada àrea de cultiu. En la present Tesi s'ha dut a terme l'estudi de l'efecte del patró sobre la qualitat físic-química i nutricional de la fruita en varietats de gran interés comercial, mandarines 'Clemenules' i 'Tango' i, taronges sanguines 'Tarocco Rosso' i 'Moro'. En 'Clemenules' es va dur a terme l'avaluació de la qualitat de la fruita d'arbres empeltats sobre huit patrons en tres moments de collita, en dues campanyes. Entre els patrons avaluats, Forner-Alcaide 13 i C-35 van destacar per avançar el canvi de color. D'altra banda Forner-Alcaide V17 va destacar per mantindre nivells òptims d'acidesa fins al final de la campanya i va presentar el major contingut en vitamina C, flavonoides, glucosa i fructosa. Carrizo Citrange també va induir altes concentracions de sacarosa i vitamina C en la fruita. 'Tango' és una mandarina de recent introducció en l'àrea mediterrània amb gran interés pel seu període de recol·lecció que comença quan finalitza el de les clementines. En la present Tesi es van estudiar els canvis en la qualitat físic-química, nutricional i sensorial de la mandarina Tango empeltada sobre dos patrons (Carrizo Citrange i Forner-Alcaide 5) durant el període de collita en les dues àrees principals de producció d'Andalusia. La qualitat de la fruita es va veure influenciada per la localització, la qual cosa es va relacionar amb la composició de la textura del sòl. En totes dues localitzacions, Forner-Alcaide 5 va ser el patró que va induir major contingut en acidesa, sòlids solubles totals, sacarosa, vitamina C i àcid cítric en la fruita. Les determinacions físic-químiques i l'avaluació sensorial van permetre establir el moment òptim de recol·lecció depenent de les diferents condicions estudiades. També s'ha inclòs un estudi del comportament postcollita d'aquesta varietat, ja que no existien dades en les nostres condicions de cultiu. La mandarina 'Tango' va presentar símptomes externs de danys per fred a partir dels 20 dies emmagatzemada a 1°C i 5°C. Es va realitzar un estudi micro-estructural per a caracteritzar l'alteració provocada per les baixes temperatures. Els fruits del patró Forner-Alcaide 5 van presentar una menor incidència dels danys per fred. L'emmagatzematge a 9°C no va comprometre la qualitat externa o interna d'aquesta varietat. Dins del grup de taronges, en els últims anys existeix una creixent demanda per les taronges sanguines pel seu consum en fresc. Per a avaluar l'efecte del patró sobre sanguines es van prendre dues varietats, 'Moro' i 'Tarocco Rosso' empeltades sobre huit patrons. La qualitat interna es va veure influenciada pel moment de collita, la qual cosa va ser més evident en la varietat 'Moro'. En totes dues varietats el patró va afectar els canvis en els paràmetres de qualitat estudiats. En 'Moro', es va observar una reducció en el color del suc degut a la degradació del antocians. Aquesta degradació es va relacionar amb el canvi de la temperatura experimentada durant el període de recol·lecció. 'Tarocco Rosso' és menys sensible als canvis de temperatura. En aquesta varietat els patrons Forner-Alcaide 5 i Forner-Alcaide 13 van produir la fruita amb major contingut en antocianos i sucres. A mes s'aporta l'estudi de l'aptitud a la frigoconservació en les sanguines 'Tarocco Rosso' i 'Sanguinelli'. Mentre que la qualitat interna no es va veure afectada per l'emmagatzematge a cap de les temperatures assajades (1°C, 5°C i 9°C) durant 45 dies, la fruita va presentar símptomes de danys per fred a 1°C. `Sanguinelli' va presentar major incidència que 'Tarocco Rosso'. La fruita es pot emmagatzemar entre 5°C i 9°C durant 30 dies en el cas de 'Sanguinelli' i fins a 45 en e
[EN] Citriculture faces changing environmental scenarios that cause biotic and abiotic stress. The rootstock onto which a specific variety is grafted is an important tool to help to improve its agronomic adaptability to each crop area. The present Thesis was carried out to study the effect of rootstock on physico-chemical and nutritional fruit quality in some varieties of commercial interest today: 'Clemenules' and 'Tango' mandarins, and 'Tarocco Rosso' and 'Moro' blood oranges. In 'Clemenules', the fruit of the trees grafted into eight rootstocks at three harvest times was evaluated by performing studies during two seasons. Forner-Alcaide 13 and C-35 Citrange stood out for their earlier color change, which is very interesting for this variety, in which early harvesting is a relevant aspect from the commercial point of view. Forner-Alcaide V17 stood out for maintaining optimum acidity levels until the season ended and presented the highest contents in vitamin C, flavonoids, glucose and fructose. Carrizo Citrange brought about high concentrations of sucrose and vitamin C in fruit. 'Tango' is a mandarin variety that has been recently introduced into the Mediterranean Region. Its harvest time is very interesting because it starts when that of clementines ends. The present Thesis studies changes in the physico-chemical, nutritional and sensorial quality of 'Tango' fruit grafted onto two rootstocks (Carrizo Citrange and Forner-Alcaide 5) during the harvest period in the two main production areas in Andalusia. The results revealed that fruit quality during harvest was influenced by the location, which was particularly related to soil texture composition. In both areas, Forner-Alcaide 5 was the rootstock that induced higher acidity content, and more total soluble solids, sucrose, vitamin C and citric acid in fruit. The physico-chemical determinations, along with the sensorial evaluation, allowed the optimum harvest time to be established depending on the different studied conditions. This Thesis also includes a study about this variety's postharvest behavior as no data are available for our crop conditions. The 'Tango' mandarin presented outer chilling injury symptoms after being stored for 20 days at 1°C and 5°C. A microstructural study was done to characterize the alteration caused by low temperatures. The Forner-Alcaide 5 rootstock fruit showed a lower chilling injury incidence. Storage at 9°C did not compromise quality fruit. Among oranges, demand for blood oranges to be eaten fresh has grown in recent years, basically due to their high content in anthocyanins and their positive effect for human health. To assess the effect that rootstock had on blood oranges, two varieties were taken, 'Moro' and 'Tarocco Rosso', grafted onto eight rootstocks. Internal quality was strongly influenced by harvest time, which was more evident for 'Moro'. In both varieties, rootstock affected changes in the quality parameter studied. In 'Moro', juice color faded as anthocyanins degraded, and rootstocks C-35 Citrange, Macrophylla and Volkameriana showed the most marked reduction. Such anthocyanin degradation was related to the change in temperature that took place during the harvest period. In 'Tarocco Rosso', anthocyanins did not undergo degradation, which suggests that this variety is less sensitive to changes in temperature. In this variety, rootstocks Forner-Alcaide 5 and Forner-Alcaide 13 gave fruit with a higher content of anthocyanins and sugars. This Thesis also includes a study of the suitability of cold storage of two blood orange varieties: 'Tarocco Rosso' and 'Sanguinelli'. Although storage at any tested temperature (1°C, 5°C and 9°C) did not affect internal quality for 45 days, fruit displayed chilling injury symptoms at 1°C, with a higher incidence for 'Sanguinelli' than for 'Tarocco Rosso'. Fruit can be stored between 5°C and 9°C for 30 days for 'Sanguinelli' and for up to 45 days with 'Tarocco Rosso'.
This study has been supported by Instituto Valenciano de Investigaciones Agrarias and co-financed by FEDER and European Social Fund. The authors thank Anecoop S. Coop. and Frutaria Agricultura, S.L for supplying the fruit herein used and its technical support.
Morales Alfaro, J. (2021). Effect of Rootstock on the Fruit Quality of Mandarins "Clemenules" and "Tango", and Blood Oranges "Tarocco Rosso" and "Moro" [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/165858
TESIS

Four 'Lisbon' lemon selections, 'Frost Nucellar', 'Corona Foothills', 'Limoneira 8A' and 'Prior' were selected for evaluation on Citrus volkameriana rootstock. 2004-05 results indicate that the 'Limoneira 8A Lisbon' and ‘Corona Foothills Lisbon’

Two orange cultivar trials have been established in Arizona, one at the Yuma Mesa Agricultural Center, Yuma, AZ and one at the Citrus Agriculture Center, Waddell, AZ. For the navel orange trial in Yuma, all the selections had improved yields in 2004-05. ‘Fisher’ navel continues to have the greatest yield, but is quite granulated. Of the rest in the Yuma trial, ‘Lane Late’ had the best quality and yield. For the Waddell trial, the fourth year data has been collected, and suggests that ‘Fisher’, ‘Beck-Earli’, ‘Chislett’ and ‘Lane Late’ are outperforming the other cultivars tested to date.

Three lemon cultivar selection trials are being conducted at the Yuma Mesa Agriculture Center in Somerton, AZ. Data from these trials suggest that ‘Limonero Fino 49’ selections may be a suitable alternative for the varieties most commonly planted in Southwest Arizona today. ‘Femminello’ and ‘Villafranca’ might also be planted on an experimental basis.

Second year yield and packout data from a trial containing ‘Fina’, ‘Fina Sodea’, ‘Sidi Aissa’, ‘Oroval’, ‘W. Murcott Afourer’, ‘Fremont’, and ‘Gold Nugget; selections were collected in 2004-05. For the year, ‘Fina Sodea’ had the greatest yield, and average fruit size, while ‘Fremont’ had the smallest yield, and the smallest fruit size.

Four 'Lisbon' lemon selections, 'Frost Nucellar', 'Corona Foothills', 'Limoneira 8A' and 'Prior' were selected for evaluation on Citrus volkameriana rootstock. 2005-06 results indicate that the 'Limoneira 8A Lisbon' and ‘Corona Foothills Lisbon’ are superior to the other two selections tested.

Two orange cultivar trials have been established in Arizona, one at the Yuma Mesa Agricultural Center, Yuma, AZ and one at the Citrus Agriculture Center, Waddell, AZ. For the navel orange trial in Yuma, all the selections had improved yields in 2005-06. ‘Fisher’ navel continues to have the greatest yield, but is quite granulated. Of the rest in the Yuma trial, ‘Lane Late’ had the best quality and yield. For the Waddell trial, the fourth year data has been collected, and suggests that ‘Fisher’, ‘Beck-Earli’, ‘Chislett’ and ‘Lane Late’ are outperforming the other cultivars tested to date.

Four 'Lisbon' lemon selections, 'Frost Nucellar', 'Corona Foothills', 'Limoneira 8A' and 'Prior' were selected for evaluation on Citrus volkameriana rootstock. Yield was extremely low for 2006-07, but these results do not change the fact that that 'Limoneira 8A Lisbon' and 'Corona Foothills Lisbon' are superior to the other two selections tested.

Three lemon cultivar selection trials are being conducted at the Yuma Mesa Agriculture Center in Somerton, AZ. Data from these trials suggest that 'Limonero Fino 49' and 'Cascade Eureka' selections may be a suitable alternative for the varieties most commonly planted in Southwest Arizona today. 'Femminello' and 'Villafranca' might also be planted on an experimental basis

First year yield and packout data from a trial containing 'Fina', 'Fina Sodea', 'Sidi Aissa', 'Oroval', 'W. Murcott Afourer', 'Fremont', and 'Gold Nugget; selections were collected in 2004-05. For the year, 'Fremont' had the greatest yield, but the smallest fruit size, while 'Fina' had the smallest yield, and 'W. Murcott Afourer' had the largest fruit size.