Journal articles on the topic 'Root-Microorganism interaction'
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1
Kumari, Pallavi, Tali Sayas, Patricia Bucki, Sigal Brown-Miyara, and Maya Kleiman. "Real-Time Visualization of Cellulase Activity by Microorganisms on Surface." International Journal of Molecular Sciences 21, no. 18 (September 9, 2020): 6593. http://dx.doi.org/10.3390/ijms21186593.
Abstract:
A variety of methods to detect cellulase secretion by microorganisms has been developed over the years, none of which enables the real-time visualization of cellulase activity on a surface. This visualization is critical to study the interaction between soil-borne cellulase-secreting microorganisms and the surface of plant roots and specifically, the effect of surface features on this interaction. Here, we modified the known carboxymethyl cellulase (CMC) hydrolysis visualization method to enable the real-time tracking of cellulase activity of microorganisms on a surface. A surface was formed using pure CMC with acridine orange dye incorporated in it. The dye disassociated from the film when hydrolysis occurred, forming a halo surrounding the point of hydrolysis. This enabled real-time visualization, since the common need for post hydrolysis dyeing was negated. Using root-knot nematode (RKN) as a model organism that penetrates plant roots, we showed that it was possible to follow microorganism cellulase secretion on the surface. Furthermore, the addition of natural additives was also shown to be an option and resulted in an increased RKN response. This method will be implemented in the future, investigating different microorganisms on a root surface microstructure replica, which can open a new avenue of research in the field of plant root–microorganism interactions.
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Antoszewski, Marcel, Agnieszka Mierek-Adamska, and Grażyna B. Dąbrowska. "The Importance of Microorganisms for Sustainable Agriculture—A Review." Metabolites 12, no. 11 (November 11, 2022): 1100. http://dx.doi.org/10.3390/metabo12111100.
Abstract:
In the face of climate change, progressive degradation of the environment, including agricultural land negatively affecting plant growth and development, endangers plant productivity. Seeking efficient and sustainable agricultural techniques to replace agricultural chemicals is one of the most important challenges nowadays. The use of plant growth-promoting microorganisms is among the most promising approaches; however, molecular mechanisms underneath plant–microbe interactions are still poorly understood. In this review, we summarized the knowledge on plant–microbe interactions, highlighting the role of microbial and plant proteins and metabolites in the formation of symbiotic relationships. This review covers rhizosphere and phyllosphere microbiomes, the role of root exudates in plant–microorganism interactions, the functioning of the plant’s immune system during the plant–microorganism interactions. We also emphasized the possible role of the stringent response and the evolutionarily conserved mechanism during the established interaction between plants and microorganisms. As a case study, we discussed fungi belonging to the genus Trichoderma. Our review aims to summarize the existing knowledge about plant–microorganism interactions and to highlight molecular pathways that need further investigation.
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Hlushach, D., V. Zhmurko, and O. Avksentieva. "Influence of genotype and bacterization on growth, development, and soluble carbohydrate content in soybean E-genes isogenic lines." Journal of V. N. Karazin Kharkiv National University, Series "Biology", no. 40 (June 26, 2023): 59–70. http://dx.doi.org/10.26565/2075-5457-2023-40-5.
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Photoperiod, which regulates the duration of vegetative and generative development, and the plant-microorganism interaction, which influences the metabolic status of plant organisms, are important factors in the regulating plant growth and development. The aim of the study was to determine the influence of Glycine max (L.) Merr. genotype and seed pre-bacterization with a virulent and active strain of Bradyrhizobium japonicum 634b on the plant growth and development, and on the soluble carbohydrate content in leaves of isogenic by E-genes lines under field conditions. Nearly isogenic lines (NILs) of soybean, in which the E1, E2, and E3 genes are located at different allelic loci, were used. Sterile seeds were pretreated with distilled water (control) and Bradyrhizobium japonicum 634b cell suspension (experiment). Plants were grown under natural long-day conditions (16 hours). The growth and development of the soybean were evaluated by phenological observations, morphometric indicators fixed at the V3 and V5 developmental stages, relative growth rate (RGR), and the content of soluble sugars ‒ mono- and oligosaccharides. The effect of the factors studied (genotype, bacterization, and their interaction) was calculated. The results of the experiment and the calculation of the effect of the factor showed that the isoline genotype has the greatest effect on seed germination, phenological development of the plant and duration of the VE-R1 phase, growth of the root system in the V3 and V5 phases, and the content of monosaccharides involved in forming the plant-microorganism interaction. The effect of bacterization is most evident in the RGR, shoot development, and the oligosaccharide content of the leaves of NILs in the V3 and V5 phases. Among the isolines studied, L 80-5879, which has the E1 gene (flowering repressor) in a dominant state, was characterized by minimal sensitivity to bacterization. It was found that bacterization and genotype interaction didn't influence the VE-R1 duration stage and the shoot and root length. The results obtained therefore prove that the E-series genes, which determine the photoperiodic sensitivity of soya beans, can also be indirectly involved in establishing plant-microorganism interactions.
4
Siswanto, U., O. D. Pusponegoro, and N. Anindyawati. "The use of cabbage compost and indigenous microorganism for cultivation of lettuce (Lactuca sativa L.)." IOP Conference Series: Earth and Environmental Science 1302, no. 1 (February 1, 2024): 012120. http://dx.doi.org/10.1088/1755-1315/1302/1/012120.
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Abstract Cabbage, a widely cultivated cruciferous vegetable, generates substantial waste material during its harvest and processing. This study was conducted to analyse the effectiveness of three decomposer types and their concentration to break down cabbage waste compost on the cultivation of lettuce (Lactuca sativa L.). The research employed randomized complete block design with two factors and three replications. The first factor was the cabbage waste compost amount: 20 g, 30 g, and 40 g per plant. The second factor was type of compost decomposers: without decomposers, EM4, Thiobacillus sp., and indigenous microorganism. Variables observed included leaf length, number of leafs, leaf fresh weight, leaf dry weight, root fresh weight, root dry weight, and root length. Data were analysed using analysis of variance and means were separated using Duncan’s multiple range test. There was no interaction between the decomposer and the amount of cabbage waste compost on all parameters. The type of cabbage waste compost decomposer had a very significant effect on the number of leaves and root length. The amount of cabbage waste compost did not significantly affect all parameters. Indigenous microorganism (IMO) decomposer resulted in the number of leaf 14.36 and leaf length of 5.79 cm.
5
Carrillo-Flores, Elizabeth, Jonanci Arreola Rivera, Denni Mariana Pazos-Solis, Moises Bocanegra-Mondragon, Grisel Fierros Romero, Maria Elena Mellado-Rojas, and Elda Beltran-Pena. "TOR participation on the root system changes of Arabidopsis during its interaction with Azospirillum." Journal of Applied Biotechnology & Bioengineering 9, no. 2 (March 7, 2022): 18–23. http://dx.doi.org/10.15406/jabb.2022.09.00280.
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The root system of the plant is essential for taking up water and nutrients, serves as an anchor and is the organ where plant-microorganism interaction takes place. When the Plant Growth Promoting Rhizobacteria (PGPR) Azospirillum brasilense Sp245 colonizes the root of the plants, it halts the growth of the primary root and stimulates the development of the lateral roots and root hairs which support vegetative, green biomass. Target of Rapamycin (TOR) is a highly conserved protein in all eukaryotes, and it controls anabolic processes, such as cell cycle, ribosome biogenesis, protein synthesis, cell wall changes and photosynthesis among others. TOR in plants forms part of the TORC1 complex, which when is activated by auxins and light, activates anabolic processes and represses autophagy. TOR regulates the growth of the primary root of Arabidopsis through cell proliferation and elongation. In the present investigation, the participation of TOR during the Arabidopsis-Azospirillum interaction was determined using two approaches, a pharmacology and other genetic. The results showed that TOR is involved in the development of the lateral roots of A. thaliana seedlings inoculated with A. brasilense.
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Kumari, Pallavi, Neta Ginzburg, Tali Sayas, Sigal Saphier, Patricia Bucki, Sigal Brown Miyara, Denise L. Caldwell, Anjali S. Iyer-Pascuzzi, and Maya Kleiman. "A biomimetic platform for studying root-environment interaction." Plant and Soil 447, no. 1-2 (December 13, 2019): 157–68. http://dx.doi.org/10.1007/s11104-019-04390-6.
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Abstract Aims Microstructure plays an important role in biological systems. Microstructural features are critical in the interaction between two biological organisms, for example, a microorganism and the surface of a plant. However, isolating the structural effect of the interaction from all other parameters is challenging when working directly with the natural system. Replicating microstructure of leaves was recently shown to be a powerful research tool for studying leaf-environment interaction. However, no such tool exists for roots. Roots present a special challenge because of their delicacy (specifically of root hairs) and their 3D structure. We aim at developing such a tool for roots. Methods Biomimetics use synthetic systems to mimic the structure of biological systems, enabling the isolation of structural function. Here we present a method which adapts tools from leaf microstructure replication to roots. We introduce new polymers for this replication. Results We find that Polyurethane methacrylate (PUMA) with fast UV curing gives a reliable replication of the tomato root surface microstructure. We show that our system is compatible with the pathogenic soilborne bacterium Ralstonia solanacearum. Conclusions This newly developed tool may be used to study the effect of microstructure, isolated from all other effects, on the interaction of roots with their environment.
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Vandana, Udaya Kumar, Jina Rajkumari, L. Paikhomba Singha, Lakkakula Satish, Hemasundar Alavilli, Pamidimarri D. V. N. Sudheer, Sushma Chauhan, et al. "The Endophytic Microbiome as a Hotspot of Synergistic Interactions, with Prospects of Plant Growth Promotion." Biology 10, no. 2 (February 1, 2021): 101. http://dx.doi.org/10.3390/biology10020101.
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The plant root is the primary site of interaction between plants and associated microorganisms and constitutes the main components of plant microbiomes that impact crop production. The endophytic bacteria in the root zone have an important role in plant growth promotion. Diverse microbial communities inhabit plant root tissues, and they directly or indirectly promote plant growth by inhibiting the growth of plant pathogens, producing various secondary metabolites. Mechanisms of plant growth promotion and response of root endophytic microorganisms for their survival and colonization in the host plants are the result of complex plant-microbe interactions. Endophytic microorganisms also assist the host to sustain different biotic and abiotic stresses. Better insights are emerging for the endophyte, such as host plant interactions due to advancements in ‘omic’ technologies, which facilitate the exploration of genes that are responsible for plant tissue colonization. Consequently, this is informative to envisage putative functions and metabolic processes crucial for endophytic adaptations. Detection of cell signaling molecules between host plants and identification of compounds synthesized by root endophytes are effective means for their utilization in the agriculture sector as biofertilizers. In addition, it is interesting that the endophytic microorganism colonization impacts the relative abundance of indigenous microbial communities and suppresses the deleterious microorganisms in plant tissues. Natural products released by endophytes act as biocontrol agents and inhibit pathogen growth. The symbiosis of endophytic bacteria and arbuscular mycorrhizal fungi (AMF) affects plant symbiotic signaling pathways and root colonization patterns and phytohormone synthesis. In this review, the potential of the root endophytic community, colonization, and role in the improvement of plant growth has been explained in the light of intricate plant-microbe interactions.
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Xiong, Qiangqiang, Jinlong Hu, Haiyan Wei, Hongcheng Zhang, and Jinyan Zhu. "Relationship between Plant Roots, Rhizosphere Microorganisms, and Nitrogen and Its Special Focus on Rice." Agriculture 11, no. 3 (March 11, 2021): 234. http://dx.doi.org/10.3390/agriculture11030234.
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Fertilization is an important practical measure in agricultural production. As an important nutrient element of plants, nitrogen (N) has a significant impact on the plant productivity and microbial function. Rhizosphere microorganisms affect plant growth and development, nitrogen uptake and utilization, and ecological adaptability. The interaction mechanism between plant and rhizosphere microorganisms is one of the hotspots in life science research and the key program of agricultural microorganism utilization. In this article, the relationship among plant root morphology and physiology, rhizosphere microorganisms, and nitrogen is reviewed, summarized, and prospected.
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Solórzano-Acosta, Richard, Marcia Toro, and Doris Zúñiga-Dávila. "Effect of Co-Inoculation with Growth-Promoting Bacteria and Arbuscular Mycorrhizae on Growth of Persea americana Seedlings Infected with Phytophthora cinnamomi." Microorganisms 12, no. 4 (April 2, 2024): 721. http://dx.doi.org/10.3390/microorganisms12040721.
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Avocado is one of the most in-demand fruits worldwide and the trend towards its sustainable production, regulated by international standards, is increasing. One of the most economically important diseases is root rot, caused by Phythopthora cinnamomi. Regarding this problem, antagonistic microorganism use is an interesting alternative due to their phytopathogen control efficiency. Therefore, the interaction of arbuscular mycorrhizal fungi of the phylum Glomeromycota, native to the Peruvian coast (GWI) and jungle (GFI), and avocado rhizospheric bacteria, Bacillus subtilis and Pseudomonas putida, was evaluated in terms of their biocontrol capacity against P. cinnamomi in the “Zutano” variety of avocado plants. The results showed that the GWI and Bacillus subtilis combination increased the root exploration surface by 466.36%. P. putida increased aerial biomass by 360.44% and B. subtilis increased root biomass by 433.85%. Likewise, P. putida rhizobacteria showed the highest nitrogen (24.60 mg ∙ g−1 DM) and sulfur (2.60 mg ∙ g−1 DM) concentrations at a foliar level. The combination of GWI and Bacillus subtilis was the treatment that presented the highest calcium (16.00 mg ∙ g−1 DM) and magnesium (8.80 mg ∙ g−1 DM) concentrations. The microorganisms’ multifunctionality reduced disease severity by 85 to 90% due to the interaction between mycorrhizae and rhizobacteria. In conclusion, the use of growth promoting microorganisms that are antagonistic to P. cinnamomi represents a potential strategy for sustainable management of avocado cultivation.
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Qi, Bianbin, Kuo Zhang, Sijun Qin, Deguo Lyu, and Jiali He. "Glucose addition promotes C fixation and bacteria diversity in C-poor soils, improves root morphology, and enhances key N metabolism in apple roots." PLOS ONE 17, no. 1 (January 19, 2022): e0262691. http://dx.doi.org/10.1371/journal.pone.0262691.
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The interaction between plant, soil and microorganism plays a crucial role in sustainable development of terrestrial ecosystem function and diversity. However, little information is known about how plant growth, soil organic carbon (C) fractions and microorganism respond to exogenous C addition in soils with low organic C content. Three levels of 13C-glucose (equal to 0, 100% and 500% of initial microbial biomass C) were added to non-sterilized (corresponding to treatment abbreviation of CK, Glu-1, Glu-2, respectively) and sterilized soils (corresponding to treatment abbreviation of SS, SS+Glu-1, SS+Glu-2, respectively) planted with apple rootstock (Malus baccata (L.) Borkh.) seedings. The objectives of this study were to analyse the dynamics of soil organic C (SOC) fractions and soil bacterial community diversity with glucose levels and soil sterilization, and to explore the morphology of roots and nitrogen (N) metabolism by plant after glucose addition to sterilized/non-sterilized soils. Results showed that the contents of labile organic C fractions were significantly varied (P<0.05) with the levels of glucose addition and soil sterilization. SS+Glu-2 and Glu-2 treatments increased the contents of labile organic C fractions, on average, by 48.47% and 35.33% compared with no glucose addition, respectively. About 21.42% and 16.17% of glucose-C remained in sterilized and non-sterilized soils, respectively at the end of experiment (day 45). Regardless of soil sterilized or not, the glucose addition increased the richness and diversity indices of soil bacterial community compared with no-glucose addition. The glucose addition optimized root zone conditions, and enhanced root vitality, morphology and biomass. Both SS+Glu-2 and Glu-2 treatments significantly enhanced (P<0.05) the contents of nitrate (NO3—N) and nitrite (NO2—N), but sharply decreased (P<0.05) the ammonium (NH4+-N) content compared with no glucose addition. Also, these two treatments significantly (P<0.05) increased the enzymic activities and gene transcript levels involved in root N metabolism, which demonstrated that the high level of glucose addition promoted N assimilation and transformation into free amino acids by root. Overall, the addition of exogenous C to not only promotes its fixation and bacterial community diversity in C-poor soils, but also improves root morphology and N absorption by plant.
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Bulanov, Alexander G., Anton A. Shagaev, Alexey A. Belov, and Nikolay S. Markvichev. "Physiological properties of resistance strain Fusarium oxysporum." Butlerov Communications 57, no. 2 (February 28, 2019): 144–50. http://dx.doi.org/10.37952/roi-jbc-01/19-57-2-144.
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Soil microorganisms have a significant effect on microbiological cenosis. Such communities are called the root micro-flora of the plant. All organisms in the microflora are divided into two groups: rhizoplane, living directly on the root system of the plant, and rhizosphere, developing in the root area of the plant. The intensive population of the root and root zones of a plant is primarily associated with the release (exosmosome) of organic substances or exudates formed during the life of the plant. The plant microflora includes not only symbiotic and commensalithic types of interaction, but also parasitic forms. Parasitic pathogens are divided into two groups: pathogenic and conditionally pathogenic. Conditionally pathogenic microorganisms are in a latent state and have an effect only when certain conditions arise for their activation and development. Such conditions may include changes in temperature, humidity, disturbance of plant homeostasis, or damage to the integument. Pathogenic microorganisms, by contrast, are always active and infect the host organism upon contact. The pathogenicity of a microorganism is a complex of traits that adversely affect the health of the plant, cause various pathologies, leading to growth inhibition and partial inhibition of the development or complete destruction of the culture. The pathogenic properties of the Fusarium oxysporum culture F201 were investigated against the cucumber culture by Atlet F1. It was shown the phytopathogenic microorganisms Fusarium oxysporum has acquired signs of resistance without lost her aggressiveness properties against a plant. Fusarium oxysporum quite comparable with the common strain.
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Savian, Francesco, Fabrizio Ginaldi, Rita Musetti, Nicola Sandrin, Giulia Tarquini, Laura Pagliari, Giuseppe Firrao, Marta Martini, and Paolo Ermacora. "Studies on the aetiology of kiwifruit decline: interaction between soil-borne pathogens and waterlogging." Plant and Soil 456, no. 1-2 (September 7, 2020): 113–28. http://dx.doi.org/10.1007/s11104-020-04671-5.
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Abstract Aims In 2012, Italian kiwifruit orchards were hit by a serious root disease of unknown aetiology (kiwifruit decline, KD) that still causes extensive damage to the sector. While waterlogging was soon observed to be associated with its outbreak, the putative role of soil microbiota remains unknown. This work investigates the role of these two factors in the onset of the disease. Methods Historical rainfall data were analysed to identify changes that might explain KD outbreak and mimic the flooding conditions required to reproduce the disease in a controlled environment. A greenhouse experiment was thus designed, and vines were grown in either unsterilized (U) or sterilized (S) soil collected from KD-affected orchards, and subjected (F) or not (N) to artificial flooding. Treatments were compared in terms of mortality rate, growth, and tissue modifications. Results KD symptoms were only displayed by FU-treated vines, with an incidence of 90%. Ultrastructural observations detected tyloses and fibrils in the xylem vessels of all plants, irrespective of the treatment. Phytopythium vexans and Phytopythium chamaehyphon, isolated from roots of FU plants, emerged as the associated microorganisms. Conclusions We succeeded in reproducing KD under controlled conditions and confirmed its association with both waterlogging and soil-borne microorganism(s).
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Corona-Sánchez, Iván, Cesar Arturo Peña-Uribe, Omar González-López, Javier Villegas, Jesus Campos-Garcia, and Homero Reyes de la Cruz. "Cyclodipeptides from Pseudomonas aeruginosa modulate the maize (Zea mays L.) root system and promote S6 ribosomal protein kinase activation." PeerJ 7 (August 28, 2019): e7494. http://dx.doi.org/10.7717/peerj.7494.
Abstract:
Background Pseudomonas aeruginosa is an opportunistic and pathogenic bacterium with the ability to produce cyclodipeptides (CDPs), which belong to a large family of molecules with important biological activities. Excessive amounts of CDPs produced by Pseudomonas strains can activate an auxin response in Arabidopsis thaliana and promote plant growth. Target of rapamycin (TOR) is an evolutionarily conserved eukaryotic protein kinase that coordinates cell growth and metabolic processes in response to environmental and nutritional signals. Target of rapamycin kinase phosphorylates various substrates, of which S6 ribosomal protein kinase (S6K) is particularly well known. The PI3K/Akt/mTOR/S6K signaling pathway has been studied extensively in mammals because of its association with fundamental biological processes including cell differentiation. However, evidences suggest that this pathway also has specific and conserved functions in plants and may thus be conserved, as are several of its components like TOR complex 1 and S6K proteins. In plants, TOR-S6K signaling has been shown to be modulated in response to plant growth promoters or stressors. Methods In this study, we evaluated the effects of P. aeruginosa CDPs on the growth and root development of maize plants (Zea mays L.) by adding different CDPs concentrations on culture plant media, as well as the effect on the phosphorylation of the maize S6K protein (ZmS6K) by protein electrophoresis and western blot. Results Our results showed that P. aeruginosa CDPs promoted maize growth and development, including modifications in the root system architecture, correlating with the increased ZmS6K phosphorylation and changes induced in electrophoretic mobility, suggesting post-translational modifications on ZmS6K. These findings suggest that the plant growth-promoting effect of the Pseudomonas genus, associated with the CDPs production, involves the TOR/S6K signaling pathway as a mechanism of plant growth and root development in plant–microorganism interaction.
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Anis, S. D., D. A. Kaligis, and S. Dalie. "PENGARUH NAUNGAN DAN LEVEL NITROGEN TERHADAP KARAKTER PERTUMBUHAN RUMPUT CORONIVA." ZOOTEC 35, no. 1 (February 6, 2015): 78. http://dx.doi.org/10.35792/zot.35.1.2015.7098.
Abstract:
EFFECT OF SHADING AND NITROGEN LEVEL TO CHARACTER OF GROWTH CORONIVA GRASS (Brachiaria humidicola).The Coroniva grass (Brachiaria humidicola) or Schweick was ruminant animal feed grass with high ability for land recovering, land erosion and high nutrient quality for ruminant animals. In the areas with high sun light and land nutrient availability,this grass was capable to increase carbon accumulation under top soil and contribute the nitrogen (N) as fixation result of Azotobacter microorganism. Under shading areas and lack of land nutrient availability, especially nitrogen, growth and production potentials had not been fully explored. The objective of this study was to evaluate the levels of shading areas and nitrogen fertilizer on growth traits, dried matter plant leaf canopy and ratio between plant canopy and plan root of Coroniva grass (Brachiaria humidicola). Treatments were divided into three levels of shading (0%, 40%, 70%) and nitrogen fertilizer levels of 0 kg, 50 kg and 100 kg ha-1. Treatments were applied using factorial design based on block randomized design. Results showed that interaction between shading area of 40% and N level of 100 kg ha-1 produced the highest numbers and length of stolon, while the highest numbers of propagating plant were found at interaction between shading area of 40% and N level of 50 kg ha-1. Therefore, it can be concluded that growth traits of Coroniva grass were determined by the factors of shading areas and nitrogen macro element. Key word: Coroniva grass, shading area, nitrogen, growth.
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Cabrera-Puerto, Roberto J., Francisco J. Ruiz-Gómez, and Rafael M. Navarro-Cerrillo. "Beneficial Microorganisms and Water Stress Influence Quercus ilex Seedlings’ Response to Phytophthora cinnamomi Rands." Forests 14, no. 5 (April 24, 2023): 870. http://dx.doi.org/10.3390/f14050870.
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Root rot affecting holm oak is a cause of high ecological and economic losses in the Iberian Peninsula, highlighting the relevance of developing disease control methods. The aim of this work was to assess the effect of a biological treatment composed of beneficial organisms (Trichoderma complex, T-complex) on holm oak seedlings infected by Phytophthora cinnamomi in two contrasted holm oak ecotypes, one considered highly susceptible (HU) and another considered tolerant to the pathogen (GR). For this purpose, a complete multifactorial test was carried out in a greenhouse, and seedlings were monitored for survival analysis and morphological and physiological attribute evaluation. Mortality began earlier in the susceptible (HU) than in the tolerant (GR) ecotype, and survival showed different trends due to the inoculation with beneficial microorganisms depending on the ecotype of the plants. The tolerant ecotype showed a high survival rate and better response to the treatment with beneficial microorganisms. GLM showed that the main reason for differences between treatments was ecotype, followed by T-complex and irrigation, and a weak interaction between ecotype and P. cinnamomi was found. The linear relationship between photosynthesis (A) and transpiration (Tr) showed an increase in the A/Tr rates for infected and inoculated plants under drought conditions for the GR ecotype. The tolerant ecotype was benefited more by the beneficial microorganism treatment. The understanding of the genetic diversity of Q. ilex and water stress influence on the efficacy of biological treatments against root rot provides useful information to develop environmentally friendly disease control methods to address the holm oak decline.
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Li, Yanan, Chengyu Wang, Junnan Wu, Yumang Zhang, Qi Li, Shuxia Liu, and Yunhang Gao. "The Effects of Localized Plant–Soil–Microbe Interactions on Soil Nitrogen Cycle in Maize Rhizosphere Soil under Long-Term Fertilizers." Agronomy 13, no. 8 (August 12, 2023): 2114. http://dx.doi.org/10.3390/agronomy13082114.
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Long-term fertilization can result in changes in the nitrogen (N) cycle in maize rhizosphere soil. However, there have been few reports on the impacts of plant–soil–microbe regulatory mechanisms on the N cycle in soil. In this study, soil samples were collected from a long-term experimental site located at Jilin Agricultural University, Changchun City, Jilin Province, Northeast China. We then analyzed the changes in the functional genes related to the N cycle, soil enzyme activity, and maize root exudates under long-term fertilizer application using metagenomics and liquid chromatography analysis. We aimed to investigate the response of the N cycle to long-term fertilizers, the interaction among plant, soil, and microbes, and the effect of the plant–soil–microbe system on the N cycle. Long-term fertilization had a significant effect on soil N contents, N2O emissions, and enzyme activity related to the N cycle in maize rhizosphere soil. The functional genes of the N cycle were mainly enriched in the N degradation pathway in maize rhizosphere soil. N fertilizer application decreased the abundance of functional genes related to N fixation and degradation, denitrification, and assimilatory nitrate reduction (ANRA) and increased the abundance of functional genes participating in dissimilatory nitrate reduction (DNRA) and anaerobic ammonia oxidation/hydroxylamine oxidation. The soil environment was positively related to soil enzyme activity and negatively related to the microbial community composition and amino acids in root exudates. The contribution rate of microorganisms to the N cycle was the highest (r2 = 0.900), followed by amino acids (r2 = 0.836) and the soil environment (r2 = 0.832). Therefore, we concluded that N fertilizer is the main factor limiting the soil N cycle and that microorganisms are the main factor regulating the N cycle in the plant–soil–microorganism system.
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May, Andre, Michelli de Souza dos Santos, Evandro Henrique Figueiredo Moura da Silva, Ronaldo da Silva Viana, Nilson Aparecido Vieira Junior, Nilza Patrícia Ramos, and Itamar Soares de Melo. "Effect of Bacillus aryabhattai on the initial establishment of pre-sprouted seedlings of sugarcane varieties." Research, Society and Development 10, no. 2 (February 7, 2021): e11510212337. http://dx.doi.org/10.33448/rsd-v10i2.12337.
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This study proposes to examine the potential use of Bacillus aryabhattai in pre-sprouted seedlings of sugarcane inoculated with the microorganism when subjected to different regimes of water supply after transplanting. The experiment was laid out in a randomized-block design with a complete 3 × 2 × 5 factorial arrangement, in five replicates. The factors were represented by pre-sprouted seedlings of three sugarcane varieties (IAC 911099, RB 855156 and CTC 20), two seedling types (inoculated and not inoculated with B. aryabhattai) and five frequencies of water supply, which provided the ability of return to 100% soil field capacity, at every 0, 10, 20, 30 and 40 days. Plant mortality, plant height, number of leaves, stalk diameter and number of tillers were evaluated throughout the experimental period. At the end of the study, the shoot dry matter (SDM) and root dry matter (RDM) contents of the plants were measured. The number of leaves on the plant was affected only in isolation, according to the cultivar, without effects of the other studied factors. There was a double interaction effect between the factors of variety and inoculation (V*I) for the variables of SDM, stalk diameter and height; and between frequency and inoculation (F*I) for stalk diameter. There was a triple interaction effect between variety, inoculation and frequency (V*I*F) for the RDM variable. Thus, the use of B. aryabhattai as an inoculant in pre-sprouted seedlings of sugarcane at the time of seedling formation can improve plant development after transplanting depending on the cultivar used, especially in IAC 911099 and RB 855156.
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Hartel, Peter G., Jean W. Billingsley, and Joseph W. Williamson. "Styrofoam Cup-Membrane Assembly for Studying Microorganism-Root Interactions." Applied and Environmental Microbiology 55, no. 5 (1989): 1291–94. http://dx.doi.org/10.1128/aem.55.5.1291-1294.1989.
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Georgieva, Slavka, Søren Christensen, and Karen Stevnbak. "Nematode succession and microfauna–microorganism interactions during root residue decomposition." Soil Biology and Biochemistry 37, no. 10 (October 2005): 1763–74. http://dx.doi.org/10.1016/j.soilbio.2005.02.010.
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Planchamp, Chantal, Dirk Balmer, Andreas Hund, and Brigitte Mauch-Mani. "A soil-free root observation system for the study of root-microorganism interactions in maize." Plant and Soil 367, no. 1-2 (October 24, 2012): 605–14. http://dx.doi.org/10.1007/s11104-012-1497-8.
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SUKUMAR, POORNIMA, VALÉRIE LEGUÉ, ALICE VAYSSIÈRES, FRANCIS MARTIN, GERALD A. TUSKAN, and UDAYA C. KALLURI. "Involvement of auxin pathways in modulating root architecture during beneficial plant-microorganism interactions." Plant, Cell & Environment 36, no. 5 (December 11, 2012): 909–19. http://dx.doi.org/10.1111/pce.12036.
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Carrillo-Flores, Elizabeth, Denni Mariana Pazos-Solis, Frida Paola Diaz-Bellacetin, Grisel Fierros-Romero, Elda Beltran-Pena, and Maria Elena Mellado-Rojas. "TOR regulates plant development and plantmicroorganism interactions." Journal of Applied Biotechnology & Bioengineering 8, no. 3 (May 7, 2021): 68–74. http://dx.doi.org/10.15406/jabb.2021.08.00255.
Abstract:
The adaptation of plants to their ever-changing environment denotes a remarkable plasticity of growth that generates organs throughout their life cycle, by the activation of a group of pluripotent cells known as shoot apical meristem and root apical meristem. The reactivation of cellular proliferation in both meristems by means of TOR, Target Of Rapamycin, depends on specific signals such as glucose and light. TOR showed a significant influence in plant growth, development and nutrient assimilation as well as in microorganism interactions such as infection resistance, plant differentiation and root node symbiosis. This review highlights the pathways and effects of TOR in the sensing of environmental signals throughout the maturing of different plant species
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Hu, Dandan, Jerry M. Baskin, Carol C. Baskin, Rong Liu, Xuejun Yang, and Zhenying Huang. "A Seed Mucilage-Degrading Fungus From the Rhizosphere Strengthens the Plant-Soil-Microbe Continuum and Potentially Regulates Root Nutrients of a Cold Desert Shrub." Molecular Plant-Microbe Interactions® 34, no. 5 (May 2021): 538–46. http://dx.doi.org/10.1094/mpmi-01-21-0014-fi.
Abstract:
Seed mucilage plays important roles in the adaptation of desert plants to the stressful environment. Artemisia sphaerocephala is an important pioneer plant in the Central Asian cold desert, and it produces a large quantity of seed mucilage. Seed mucilage of A. sphaerocephala can be degraded by soil microbes, but it is unknown which microorganisms can degrade mucilage or how the mucilage-degrading microorganisms affect rhizosphere microbial communities or root nutrients. Here, mucilage-degrading microorganisms were isolated from the rhizosphere of A. sphaerocephala, were screened by incubation with mucilage stained with Congo red, and were identified by sequencing and phylogenetic analyses. Fungal-bacterial networks based on high-throughput sequencing of rhizosphere microbes were constructed to explore the seasonal dynamic of interactions between a mucilage-degrading microorganism and its closely related microorganisms. The structural equation model was used to analyze effects of the mucilage-degrading microorganism, rhizosphere fungal-bacterial communities, and soil physicochemical properties on root C and N. The fungus Phanerochaete chrysosporium was identified as a mucilage-degrading microorganism. Relative abundance of the mucilage-degrading fungus (MDF) was highest in May. Subnetworks showed that the abundance of fungi and bacteria closely related to the MDF also were highest in May. Interactions between the MDF and related fungi and bacteria were positive, which might enhance mucilage degradation. In addition, the MDF might regulate root C and N by affecting rhizosphere microbial community structure. Our results suggest that MDF from the rhizosphere strengthens the plant-soil-microbe continuum, thereby potentially regulating microbial interactions and root nutrients of A. sphaerocephala. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
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Borko, Yu P., M. V. Patyka, M. V. Boiko, A. M. Honchar, and V. M. Sinchenko. "The Features of Taxonomic Structure Formation of Soil Microbial Biome in Beta vulgaris Rhizosphere." Mikrobiolohichnyi Zhurnal 84, no. 1 (April 17, 2021): 3–14. http://dx.doi.org/10.15407/microbiolj84.01.003.
Abstract:
The necessity to increase the production of quality agricultural products in order to minimize using of agrochemicals while maintaining high profitability of production are required a comprehensive study of the determining factor of soil fertility – its biological component. Research of the microbiocenoses formation in the plants rhizosphere at all ontogenesis stages will allow to uncover the mechanisms of microbial-plant interaction and develop effective ways to increase crop productivity with high functional activity and homeostasis of the soil microbiome. The goal is to study the structure of the microbial complex and biodiversity of Beta vulgaris rhizosphere during ontogenesis by classical microbiological and molecular-biological methods. Methods. The number of microorganisms was determined by the method of inoculation soil microbial suspension on agar nutrient media, the structure of the qualitative composition of microorganisms was identified by morphologically-cultural properties, the morphology of isolated isolates – by microscopy of fixed preparations. The diversity of soil microbial complexes was evaluated by the Shannon, Simpson, and Berger-Parker ecological indices. The taxonomic structure of prokaryotes was determined by pyrosequencing. Results. The differentiation of the soil microbiota number was observed during the Beta vulgaris ontogenesis due to the intensity production of root exudates by the plant. The number of bacteria and micromycetes are increased 1.8–2.3 times, however, in the phase of leaves closing in-row spacing, the number of fungal microbiota decreased by 46.4%. Microbial complexes were differed in the number of detected morphotypes (27–50) and in the structure of the distribution of dominant forms (the total number of dominant forms of bacteria was decreased during the growing season, micromycetes – was increased). Analysis of the prokaryotes metagenome by pyrosequencing made it possible to identify 214 operational taxonomic units, 10.1% of which are forms that are not cultivated on nutrient media, 23.3% are unclassified. Among the identified taxonomic units, 96.2% were identified at the order level, 85.7% – at the family level, 76.7% – at the genus level. Among the identified taxonomic units were 15 phyla bacteria and 1 – archaea, among which 96 taxonomic units, families – 167, genera – 214 we found at the level of microbial orders. The dominant forms among the identified phyla were Proteobacteria (65.7%) and Actinobacteria (20.5%); orders – Burkholderiales (38.7%) and Pseudomonadales (20.1%); families – Alcaligenacea (37.9%), Pseudomonadaceae (20.1 %); Gaiellaceae (5.7%), Nitrososphaeraceae (4.2%); genera – Achromobacter (31.5%) and Pseudomonas (19.9%). The soil microbial complex was characterized by high biodiversity according to the indicators According to the indicators of ecological indices, determined on the basis of the results of classical microbiological and molecular biological research methods, it is established that the microbial complex of the soil was characterized by high biodiversity. Although the Shannon (ISh=5.36) and Simpson (IS=0.87) indexes, based on the pyrosequencing method results, were significantly higher than similar indicators identified by classical microbiological methods. Conclusions. During the ontogenesis of Beta Vulgaris, including due to the intensity of plant production of root exudates, the number of bacteria and micromycetes in the rhizosphere of plants increased. It was accompanied by a redistribution of structural composition and an increase of the microorganisms’ diversity (ISh=5.36). It found that among the identified 214 taxonomic units, 10.1% – forms that are not cultivated on nutrient media, 23.3% – are unclassified. Our studies showed that the structure of the microbial complex of the plants’ rhizosphere reflects the characteristics of the soil and can be used as an indicator of ecological status. The obtained results (conducted for the first time in the Forest-Steppe of Ukraine) deepen the knowledge about the true scale of natural genetic diversity of microbial complexes and are a valuable asset for substantiating practical proposals for effective adaptive interactions in the plant-microorganism system to preserve the homeostasis agroecosystems.
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Battaglia, Donatella, Simone Bossi, Pasquale Cascone, Maria Cristina Digilio, Juliana Duran Prieto, Paolo Fanti, Emilio Guerrieri, et al. "Tomato Below Ground–Above Ground Interactions: Trichoderma longibrachiatum Affects the Performance of Macrosiphum euphorbiae and Its Natural Antagonists." Molecular Plant-Microbe Interactions® 26, no. 10 (October 2013): 1249–56. http://dx.doi.org/10.1094/mpmi-02-13-0059-r.
Abstract:
Below ground and above ground plant–insect–microorganism interactions are complex and regulate most of the developmental responses of important crop plants such as tomato. We investigated the influence of root colonization by a nonmycorrhizal plant-growth-promoting fungus on direct and indirect defenses of tomato plant against aphids. The multitrophic system included the plant Solanum lycopersicum (‘San Marzano nano’), the root-associated biocontrol fungus Trichoderma longibrachiatum strain MK1, the aphid Macrosiphum euphorbiae (a tomato pest), the aphid parasitoid Aphidius ervi, and the aphid predator Macrolophus pygmaeus. Laboratory bioassays were performed to assess the effect of T. longibrachiatum MK1, interacting with the tomato plant, on quantity and quality of volatile organic compounds (VOC) released by tomato plant, aphid development and reproduction, parasitoid behavior, and predator behavior and development. When compared with the uncolonized controls, plants whose roots were colonized by T. longibrachiatum MK1 showed quantitative differences in the release of specific VOC, better aphid population growth indices, a higher attractiveness toward the aphid parasitoid and the aphid predator, and a quicker development of aphid predator. These findings support the development of novel strategies of integrated control of aphid pests. The species-specific or strain-specific characteristics of these below ground–above ground interactions remain to be assessed.
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Silva, Christiana de F. B. da, Thaís L. de Brito, Carlos A. K. Taniguchi, Larissa A. Lopes, Gustavo A. S. Pinto, and Ana C. P. P. de Carvalho. "Growth-promoting potential of bacterial biomass in the banana micropropagated plants." Revista Brasileira de Engenharia Agrícola e Ambiental 22, no. 11 (November 2018): 782–87. http://dx.doi.org/10.1590/1807-1929/agriambi.v22n11p782-787.
Abstract:
ABSTRACT In the banana production system, a sustainable alternative for producing quality plantlets would be inoculation with plant growth-promoting bacteria (PGPB). Therefore, this study aimed to evaluate the growth-promoting potential of a bacterial biomass in micropropagated banana plantlets cultivar Prata Catarina, and to identify the mechanisms involved in plant-microorganism interactions. In vitro, the biochemical assays tested were the solubilisation of phosphates, production of enzymes, production of ammonia, siderophores, and indole acetic acid. In the in vivo tests, the plants were bacterised (109CFU mL-1) in two phases: acclimatisation, and cultivation in plastic bags. The design was a randomised block with 9 and 7 repetitions per treatment, which were: T1: control; T2: plants treated with isolate E2 (Bacillus pumilus group); T3: plants treated with RAB9 isolate (B. pumilus) for each phase. Bacterial isolates were capable of producing cellulases, amylases, pectinases, lipases, proteases, and siderophores. The plants gained in height, root length, root dry mass, pseudostem diameter, and leaf area. It is concluded that the PGPB can promote the growth of micropropagated banana plantlets through the production of enzymes and siderophores.
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Bajracharya, Anup Muni. "Plant growth promoting rhizobacteria (PGPR): Biofertiliser and Biocontrol agent-Review article." Journal of Balkumari College 8 (December 31, 2019): 42–45. http://dx.doi.org/10.3126/jbkc.v8i0.29304.
Abstract:
Good health starts with good food. Humans expect agriculture to supply good food with sufficient nutrients, economically and culturally valued foods, fibers and other products. But the excessive application of synthetic pesticides has exerted an adverse effect on bio-flora, fauna and natural enemies. Even a largest part of yield has been lost due to various stresses, like biotic and abiotic stresses to the plant. On this account, plant growth promoting rhizobacteria (PGPR), an eco-friendly biopesticides is boon for the biocontrol of different plant pathogens. Moreover, PGPR strains can enhance the plant growth through the production of various plant growth promoting substances. These are generally a group of microorganism that is found either in the plane of the rhizosphere or above roots impacting some positive benefits to plants. PGPR are associated with plant roots and augment plant productivity and immunity; however, recent work by several groups shows that PGPR also elicit so-called 'induced systemic tolerance' to salt and drought. PGPR might also increase nutrient uptake from soils, thus reducing the need for fertilizers and preventing the accumulation of nitrates and phosphates in agricultural soils. Scientific researches involve multidisciplinary approaches to understand adaptation of PGPR, effects on plant physiology and growth, induced systemic resistance, biocontrol of plant pathogens, bio fertilization, and potential green alternative for plant productivity, viability of co inoculating, plant microorganism interactions, and mechanisms of root colonization.
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Indriani, Lisa, Muhammad Basir Chio, Aiyen Tjoa, and Andri Tanra Tellu. "BIOKIMIAWI RIZOSFER BIBIT ROTAN PADA PEMUPUKAN NITROGEN DARI SUMBER BERBEDA." Jurnal Agrotech 10, no. 1 (June 30, 2020): 15–20. http://dx.doi.org/10.31970/agrotech.v10i1.48.
Abstract:
Root and soil interactions will alter the biochemical status of the rhizosphere, the acquisition of nutrients and impacted to plant growth. The biochemical of the rhizosphere is strongly influenced by the agriculture input e.g. fertilizer, herbicides and farm management system. Application of fertilizers, their effects are robust and difficult tobe quantified. The effect of fertilizer on the biochemical status rhizosphere and plant growth is different depending on the type of fertilizers, growth media, plant species, and also the environment condition, while the effect on plants will be specific to each plant. This research investigated on the effect of N inorganic fertilizer inputs from two sources namely Urea (CO(NH2)2) and Ammonium Sulfate or ZA ((NH4)2.SO4), and different fertilization method (homogenous and localized). Experiments showed application of N-Urea and N-ZA fertilizers reduced the rhizosphere pH until 1,4 point. However, acidity of the rhizosphere reduced microorganism colonies and respiration rate.
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Jasinski, Michal, Joanna Banasiak, Marcin Radom, Anna Kalitkiewicz, and Marek Figlerowicz. "Full-Size ABC Transporters from the ABCG Subfamily in Medicago truncatula." Molecular Plant-Microbe Interactions® 22, no. 8 (August 2009): 921–31. http://dx.doi.org/10.1094/mpmi-22-8-0921.
Abstract:
Full-size ATP-binding cassette (ABC) transporters belonging to the ABCG subfamily are unique for plants and fungi. There is growing evidence that certain of these proteins play a role in plant defense or signaling systems. As yet, a complete set of full-size ABCG protein genes has been inventoried and classified in only two plants: Arabidopsis thaliana and Oryza sativa. Recently, a domain-based clustering analysis has predicted the presence of at least 12 genes encoding such proteins in the Lotus japonicus genome. Here, we identify and classify 19 genes coding full-size ABCG proteins in Medicago truncatula, a model legume plant. We have found that the majority of these genes are expressed in roots and flowers whereas only a few are expressed in leaves. Expression of several has been induced upon pathogenic infection in both roots and leaves. ABCG messenger RNAs have been detected in root nodules forming during symbiosis of legume plants and nitrogen-fixing bacteria. The data presented provide a scaffold for further studies of the physiological function of Medicago ABCG transporters and their possible role in modulating plant–microorganism interactions.
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Oraekei, Daniel Ikechukwu, Sonne Ikechukwu Mbagwu, and Kelechukwu Emmanuel Iduma. "Evaluation of the pharmacological interactions between coadministered Newbouldia laevis root bark extract and omeprazole in ulcer induced rat model." Journal of Current Biomedical Research 2, no. 6, November-December (December 31, 2022): 659–67. http://dx.doi.org/10.54117/jcbr.v2i6.7.
Abstract:
Ulcers are wounds on the lining of the gastrointestinal tract. There are different types of ulcers some of which include: duodenal, gastric and esophageal ulcers. The most common type of ulcers is peptic ulcers which occur mostly in those portions that come into contact with stomach acids and enzymes. Other causes of ulcers comprise antibiotics, anti-inflammatory drugs, alcohol and a microorganism called Helicobacter pylori. There are numerous antiulcer drugs in current use. These drugs are associated with many problems some of which are life-threatening. In other to avert these complications, herbal remedies are used recently because they are expected to be more efficacious and with high safety potentials. In this study, we evaluated the antiulcer interactions between coadministered Newbouldia laevis root bark extract and Omeprazole using rat model. The 12 mature Wister rats used in this study were divided into four groups of three rats each. Distilled water, Omeprazole, extract of Newbouldia laevis alone and coadministration of Omeprazole and Newbouldia laevis extract were given to groups I, II, III and IV respectively. After 30 minutes, ulcer was induced in all the rats using 95% ethanol. One hour later, all the rats were sacrificed and the gastrointestinal tract separated. The pH of gastrointestinal content was assessed and the number of ulcers counted. The least number of ulcers were observed in group IV. It was concluded that Newbouldia laevis and Omeprazole interacted synergistically because they, in combination, exhibited better ulcer protective effects than each of the treatments given alone.
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Yedidia, Iris, Michal Shoresh, Zohar Kerem, Nicole Benhamou, Yoram Kapulnik, and Ilan Chet. "Concomitant Induction of Systemic Resistance to Pseudomonas syringae pv. lachrymans in Cucumber by Trichoderma asperellum (T-203) and Accumulation of Phytoalexins." Applied and Environmental Microbiology 69, no. 12 (December 2003): 7343–53. http://dx.doi.org/10.1128/aem.69.12.7343-7353.2003.
Abstract:
ABSTRACT Most studies on the reduction of disease incidence in soil treated with Trichoderma asperellum have focused on microbial interactions rather than on plant responses. This study presents conclusive evidence for the induction of a systemic response against angular leaf spot of cucumber (Pseudomonas syringae pv. lachrymans) following application of T. asperellum to the root system. To ascertain that T. asperellum was the only microorganism present in the root milieu, plants were grown in an aseptic hydroponic growth system. Disease symptoms were reduced by as much as 80%, corresponding to a reduction of 2 orders of magnitude in bacterial cell densities in leaves of plants pretreated with T. asperellum. As revealed by electron microscopy, bacterial cell proliferation in these plants was halted. The protection afforded by the biocontrol agent was associated with the accumulation of mRNA of two defense genes: the phenylpropanoid pathway gene encoding phenylalanine ammonia lyase (PAL) and the lipoxygenase pathway gene encoding hydroxyperoxide lyase (HPL). This was further supported by the accumulation of secondary metabolites of a phenolic nature that showed an increase of up to sixfold in inhibition capacity of bacterial growth in vitro. The bulk of the antimicrobial activity was found in the acid-hydrolyzed extract containing the phenolics in their aglycone form. High-performance liquid chromatography analysis of phenolic compounds showed a marked change in their profile in the challenged, preelicited plants relative to that in challenged controls. The results suggest that similar to beneficial rhizobacteria, T. asperellum may activate separate metabolic pathways in cucumber that are involved in plant signaling and biosynthesis, eventually leading to the systemic accumulation of phytoalexins.
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Vera-García, Sara-Luz, Felipe-Neri Rodríguez-Casasola, Josefina Barrera-Cortés, Arnulfo Albores-Medina, Karla M. Muñoz-Páez, Rosa-Olivia Cañizares-Villanueva, and Ma Carmen Montes-Horcasitas. "Enhancing Phosphorus and Nitrogen Uptake in Maize Crops with Food Industry Biosolids and Azotobacter nigricans." Plants 12, no. 17 (August 25, 2023): 3052. http://dx.doi.org/10.3390/plants12173052.
Abstract:
The problem of phosphorus and nitrogen deficiency in agricultural soils has been solved by adding chemical fertilizers. However, their excessive use and their accumulation have only contributed to environmental contamination. Given the high content of nutrients in biosolids collected from a food industry waste treatment plant, their use as fertilizers was investigated in Zea mays plants grown in sandy loam soil collected from a semi-desert area. These biosolids contained insoluble phosphorus sources; therefore, given the ability of Azotobacter nigricans to solubilize phosphates, this strain was incorporated into the study. In vitro, the suitable conditions for the growth of Z. mays plants were determined by using biosolids as a fertilizer and A. nigricans as a plant-growth-promoting microorganism; in vitro, the ability of A. nigricans to solubilize phosphates, fix nitrogen, and produce indole acetic acid, a phytohormone that promotes root formation, was also evaluated. At the greenhouse stage, the Z. mays plants fertilized with biosolids at concentrations of 15 and 20% (v/w) and inoculated with A. nigricans favored the development of bending strength plants, which was observed on the increased stem diameter (>13.5% compared with the negative control and >7.4% compared with the positive control), as well as a better absorption of phosphorus and nitrogen, the concentration of which increased up to 62.8% when compared with that in the control treatments. The interactions between plants and A. nigricans were observed via scanning electron microscopy. The application of biosolids and A. nigricans in Z. mays plants grown in greenhouses presented better development than when Z. mays plants were treated with a chemical fertilizer. The enhanced plant growth was attributed to the increase in root surface area.
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Giacone, Lucía, Estefanía Cordisco, María Clara Garrido, Elisa Petenatti, and Maximiliano Sortino. "Photodynamic activity of Tagetes minuta extracts against superficial fungal infections." Medical Mycology 58, no. 6 (November 14, 2019): 797–809. http://dx.doi.org/10.1093/mmy/myz114.
Abstract:
Abstract Candida and dermatophyte species are the most common causes of superficial mycoses because their treatment can be difficult due to limitations of current antifungal drugs in terms of toxicity, bioavailability, interactions, narrow-spectrum activity, and development of resistance. Photodynamic therapy (PDT) involves the topical administration of a photosensitizer in combination with light of an appropriate wavelength and molecular oxygen that produces reactive oxygen species (ROS), which promote damage to several vital components of the microorganism. Tagetes species are known as a source of thiophenes, biologically active compounds whose antifungal activity is enhanced by irradiation with UVA. The present investigation evaluated Tagetes minuta extracts as a photosensitizer on growth of Candida and dermatophytes and their effect on Candida virulence factors. T. minuta root hexane and dichloromethane extracts demonstrated high photodynamic antifungal activity. Bioautographic assays and chromatographic analysis revealed the presence of five thiophenes with reported photodynamic antifungal activities under UVA. Analysis of ROS production indicated that both type I and II reactions were involved in the activity of the extracts. In addition, the extracts inhibited virulence factors of Candida, such as adherence to epithelial surfaces and germ tube formation and showed efficacy against different Candida morphologies: budding cells, cells with germ tube and biofilms. Results suggested that PDT with T. minuta extracts might become a valuable alternative to the already established antifungal drugs for the treatment of superficial fungal infections.
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Wilkes, Thomas I., Douglas J. Warner, Veronica Edmonds-Brown, Keith G. Davies, and Ian Denholm. "The Tripartite Rhizobacteria-AM Fungal-Host Plant Relationship in Winter Wheat: Impact of Multi-Species Inoculation, Tillage Regime and Naturally Occurring Rhizobacteria Species." Plants 10, no. 7 (July 2, 2021): 1357. http://dx.doi.org/10.3390/plants10071357.
Abstract:
Soils and plant root rhizospheres have diverse microorganism profiles. Components of this naturally occurring microbiome, arbuscular mycorrhizal (AM) fungi and plant growth promoting rhizobacteria (PGPR), may be beneficial to plant growth. Supplementary application to host plants of AM fungi and PGPR either as single species or multiple species inoculants has the potential to enhance this symbiotic relationship further. Single species interactions have been described; the nature of multi-species tripartite relationships between AM fungi, PGPR and the host plant require further scrutiny. The impact of select Bacilli spp. rhizobacteria and the AM fungus Rhizophagus intraradices as both single and combined inoculations (PGPR[i] and AMF[i]) within field extracted arable soils of two tillage treatments, conventional soil inversion (CT) and zero tillage (ZT) at winter wheat growth stages GS30 and GS39 have been conducted. The naturally occurring soil borne species (PGPR[s] and AMF[s]) have been determined by qPCR analysis. Significant differences (p < 0.05) were evident between inocula treatments and the method of seedbed preparation. A positive impact on wheat plant growth was noted for B. amyloliquefaciens applied as both a single inoculant (PGPR[i]) and in combination with R. intraradices (PGPR[i] + AMF[i]); however, the two treatments did not differ significantly from each other. The findings are discussed in the context of the inocula applied and the naturally occurring soil borne PGPR[s] present in the field extracted soil under each method of tillage.
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Pokluda, Robert, Lucia Ragasová, Miloš Jurica, Andrzej Kalisz, Monika Komorowska, Marcin Niemiec, and Agnieszka Sekara. "Effects of growth promoting microorganisms on tomato seedlings growing in different media conditions." PLOS ONE 16, no. 11 (November 3, 2021): e0259380. http://dx.doi.org/10.1371/journal.pone.0259380.
Abstract:
Plant growth-promoting microbes (PGPM) play vital roles in maintaining crop fitness and soil health in stressed environments. Research have included analysis-based cultivation of soil-microbial-plant relationships to clarify microbiota potential. The goal of the research was to (i) evaluate the symbiotic microorganism effects on tomato seedling fitness under stressed conditions simulating a fragile soil susceptible to degradation; (ii) compare the plant-microbial interactions after inoculation with microbial isolates and fungi-bacteria consortia; (iii) develop an effective crop-microbial network, which improves soil and plant status. The experimental design included non-inoculated treatments with peat and sand at ratios of 50:50, 70:30, 100:0 (v:v), inoculated treatments with arbuscular mycorrhizal fungi (AMF) and Azospirillum brasilense (AZ) using the aforementioned peat:sand ratios; and treatment with peat co-inoculated with AMF and Saccharothrix tamanrassetensis (S). AMF + AZ increased root fresh weight in peat substrate compared to the control (4.4 to 3.3 g plant–1). An increase in shoot fresh weight was detected in the AMF + AZ treatment with a 50:50 peat:sand ratio (10.1 to 8.5 g plant-1). AMF + AZ reduced antioxidant activity (DPPH) (18–34%) in leaves, whereas AMF + S had the highest DPPH in leaves and roots (45%). Total leaf phenolic content was higher in control with a decreased proportion of peat. Peroxidase activity was enhanced in AMF + AZ and AMF + S treatments, except for AMF + AZ in peat. Microscopic root assays revealed the ability of AMF to establish strong fungal-tomato symbiosis; the colonization rate was 78–89%. AMF + AZ accelerated K and Mg accumulation in tomato leaves in treatments reflecting soil stress. To date, there has been no relevant information regarding the successful AMF and Saccharothrix co-inoculation relationship. This study confirmed that AMF + S could increase the P, S, and Fe status of seedlings under high organic C content conditions. The improved tomato growth and nutrient acquisition demonstrated the potential of PGPM colonization under degraded soil conditions.
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Deepika Yadav, Ramana Rao KV, Ayushi Trivedi Ayu, Yogesh Rajwade, and Neelendra Verma. "Reflective mulch films a boon for enhancing crop production: A review." Environment Conservation Journal 24, no. 1 (January 15, 2023): 281–87. http://dx.doi.org/10.36953/ecj.12962367.
Abstract:
Mulches are materials that are put to the surface of the soil for a variety of reasons. Plastic mulches of various colours have been developed and employed in a variety of agricultural production methods. Colored plastic mulches are used to modify the radiation budget and prevent soil water loss. It also helps with weed and insect infestation, as well as soil temperature regulation, water efficiency, plant development, yield, and quality. This paper reviews and discusses the understanding and prospective application of coloured plastic mulches to improve soil physical attributes growth, yield, and crop quality. Colored plastic mulches' effectiveness in minimising the detrimental impacts of environmental stress on crops is also explored. The impact of coloured plastic mulches on soil temperature has been documented by several researchers to vary from area to area and crop to crop. Various physicochemical mechanisms have also described that result in increased crop productivity when coloured plastic mulches are employed. Colored plastic mulches have a significant impact on soil temperature, moisture, and water holding capacity, according to the study. Clear and white plastics lower the temperature of the soil, but black and blue plastics raise it. A higher number of fruits, roots, tubers, and bulbs were produced when coloured plastic mulches were used. TSS, Vitamin C, and the proportion of liquid in diverse plants all improved significantly. Infestation of weeds and viral diseases has also been found to be significantly reduced. Reduced plant growth and yield, increased pest infestation, microplastic pollution, soil puddling, soil structural loss, and reduced soil-microorganism activity are some of the disadvantages of coloured plastic mulches. As a result, using coloured plastic mulches demands a detailed investigation of interactions with factors such as cropping season, root zone temperature, crop type, insect pest infestation, and water use efficiency.
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Daguerre, Yohann, Veronica Basso, Sebastian Hartmann-Wittulski, Romain Schellenberger, Laura Meyer, Justine Bailly, Annegret Kohler, Jonathan M. Plett, Francis Martin, and Claire Veneault-Fourrey. "The mutualism effector MiSSP7 of Laccaria bicolor alters the interactions between the poplar JAZ6 protein and its associated proteins." Scientific Reports 10, no. 1 (November 23, 2020). http://dx.doi.org/10.1038/s41598-020-76832-6.
Abstract:
AbstractDespite the pivotal role of jasmonic acid in the outcome of plant-microorganism interactions, JA-signaling components in roots of perennial trees like western balsam poplar (Populus trichocarpa) are poorly characterized. Here we decipher the poplar-root JA-perception complex centered on PtJAZ6, a co-repressor of JA-signaling targeted by the effector protein MiSSP7 from the ectomycorrhizal basidiomycete Laccaria bicolor during symbiotic development. Through protein–protein interaction studies in yeast we determined the poplar root proteins interacting with PtJAZ6. Moreover, we assessed via yeast triple-hybrid how the mutualistic effector MiSSP7 reshapes the association between PtJAZ6 and its partner proteins. In the absence of the symbiotic effector, PtJAZ6 interacts with the transcription factors PtMYC2s and PtJAM1.1. In addition, PtJAZ6 interacts with it-self and with other Populus JAZ proteins. Finally, MiSSP7 strengthens the binding of PtJAZ6 to PtMYC2.1 and antagonizes PtJAZ6 homo-/heterodimerization. We conclude that a symbiotic effector secreted by a mutualistic fungus may promote the symbiotic interaction through altered dynamics of a JA-signaling-associated protein–protein interaction network, maintaining the repression of PtMYC2.1-regulated genes.
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Zhang, Yi, Ying-Zhong Xie, Hong-Bin Ma, Juan Zhang, Le Jing, Yu-Tao Wang, and Jian-Ping Li. "Responses of soil microorganisms to simulated climate change in desert grassland in northern China." Journal of Water and Climate Change, March 3, 2022. http://dx.doi.org/10.2166/wcc.2022.365.
Abstract:
Abstract The study evaluates how simulated climate change affects microorganism communities in the desert grassland of Ningxia Autonomous Region, China. It explores the soil microorganism community and relationships among the soil microorganism community, chemical properties, soil respiration (SR), and plant biomass under climate change. We established a field experiment with five levels of rainfall using rainout shelters and two levels of temperature by the Open-Top Chamber (OTC). The results show that in fungal communities, normal precipitation will promote the number and base number of valid sequences the most, and R66 will significantly promote the mean length of the valid sequence. In the bacterial communities, the interaction of increasing temperature and R133 will promote the number of valid sequences and R166 will promote the length of valid sequences. Neither rainfall nor rising temperature promotes not only the soil community α-diversity but also the soil microorganism community β-diversity. Soil microorganism communities show resistance to rainfall. SR will limit the soil microorganism diversity. Soil organic carbon (SOC), soil total nitrogen (STN), and soil total phosphorus (STP) will promote soil microorganism abundance (SMA) and soil microorganism diversity (SMS). Aboveground living biomass (ALB) and soil temperature (ST) will promote soil α-diversity, whereas the effect of root biomass (RB) on the soil α-diversity is the opposite.
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Wang, Hao, Kexin Zhang, Lin Gan, Jiaqin Liu, and Guoxiong Mei. "Expansive soil-biochar-root-water-bacteria interaction: Investigation on crack development, water management and plant growth in green infrastructure." International Journal of Damage Mechanics, December 1, 2020, 105678952097441. http://dx.doi.org/10.1177/1056789520974416.
Abstract:
The objectives of the study are to explore fundamental mechanism of expansive soil-biochar-root-water-bacteria interaction, and investigate crack development and hydraulic properties of biochar amended soils aiming at green infrastructures. The physical, chemical and biological effects of biochar on expansive soil have been comprehensively explored. Crack development is investigated quantificationally, and mechanism of soil damage evolution is briefly discussed base on micro-chemical analyses. During outdoor vegetation period, photosynthesis light response curves were measured to evaluate plant growth. After period of vegetation, hydraulic properties of root-soil composites and unplanted soils were compared. The study reveals that soil crack intensity factor decreases by 33.5%, 48.5% and 47.3% due to 5%, 10% and 15% biochar introduction respectively after 5 wetting-drying cycles. 15% biochar amendment helps to restrain both initiation and propagation of soil cracks. Biochar amendment of up to 5% contributes well to residual water content and plant growth (i.e., light saturation point and light compensation point). Excessive biochar addition would restrain roots elongation, and increase saturated water content. Spatial root distribution is changed due to biochar addition, which further influences hydraulic properties and crack development. Hydraulic conductivity and soil dry density share negative correlations, 5% biochar enhances hydraulic conductivity remarkably at relatively loose condition. Biochar amendment also contributes to preventing nitrogen loss and forming more complex bacterial community in soils. The study adds to our knowledge of physio-chemical interactions of biochar with expansive clay, vegetation, water and microorganism.
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Kuppe, Christian W., Andrea Schnepf, Eric von Lieres, Michelle Watt, and Johannes A. Postma. "Rhizosphere models: their concepts and application to plant-soil ecosystems." Plant and Soil, April 19, 2022. http://dx.doi.org/10.1007/s11104-021-05201-7.
Abstract:
Abstract Background The rhizosphere is the influence-sphere of the root. It is a local ecosystem with complex functions that determine nutrient uptake, cycling of resources, and plant health. Mathematical models can quantitatively explain and help to understand rhizosphere complexity. To interpret model predictions and relevance of processes, we require understanding of the underlying concepts. Conceptualization of rhizosphere processes bridges mathematical modeling and experimental work and thus is key to understanding the rhizosphere. Scope We review concepts and assumptions foundational to the modeling of soil-plant-microorganism processes in the rhizosphere. Rhizosphere models are designed to simulate a plurality of components (solutes, substrates, and microorganisms). They specify components and interactions, drawing from the disciplines of soil science, botany, microbiology, and ecology. Solute transport models are applied to describe bioavailability in the rhizosphere. The root is typically a sink (e.g. nutrient uptake) or source (e.g. exudation) for one or more solutes. Microorganisms are usually described in time only, neglecting possible spatial movement. Interactions between components, e.g. chemical reactions and substrate-dependent bacterial growth rates, are usually described by coupling via reaction terms. Conclusions Rhizosphere models share concepts that we organized in a collective framework. This collective framework facilitates the development of new models. The interdisciplinary approach in which knowledge from soil ecology, botany, and soil physics are combined in rhizosphere models has proven fruitful for applications in plant and soil systems. We advocate multi-component-multi-interaction ecosystems around the root, with each component represented by an advection-diffusion-motility-reaction equation.
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Saini, Manish Ranjan, Latha P. Chandran, Kalyani Makarand Barbadikar, Amitha Mithra V. Sevanthi, Gautam Chawla, Megha Kaushik, Ekta Mulani, et al. "Understanding plant–microbe interaction of rice and soybean with two contrasting diazotrophic bacteria through comparative transcriptome analysis." Frontiers in Plant Science 13 (November 18, 2022). http://dx.doi.org/10.3389/fpls.2022.939395.
Abstract:
Understanding the beneficial plant–microbe interactions is becoming extremely critical for deploying microbes imparting plant fitness and achieving sustainability in agriculture. Diazotrophic bacteria have the unique ability to survive without external sources of nitrogen and simultaneously promote host plant growth, but the mechanisms of endophytic interaction in cereals and legumes have not been studied extensively. We have studied the early interaction of two diazotrophic bacteria, Gluconacetobacter diazotrophicus (GAB) and Bradyrhizobium japonicum (BRH), in 15-day-old seedlings of rice and soybean up to 120 h after inoculation (hai) under low-nitrogen medium. Root colonization of GAB in rice was higher than that of BRH, and BRH colonization was higher in soybean roots as observed from the scanning electron microscopy at 120 hai. Peroxidase enzyme was significantly higher at 24 hai but thereafter was reduced sharply in soybean and gradually in rice. The roots of rice and soybean inoculated with GAB and BRH harvested from five time points were pooled, and transcriptome analysis was executed along with control. Two pathways, “Plant pathogen interaction” and “MAPK signaling,” were specific to Rice-Gluconacetobacter (RG), whereas the pathways related to nitrogen metabolism and plant hormone signaling were specific to Rice-Bradyrhizobium (RB) in rice. Comparative transcriptome analysis of the root tissues revealed that several plant–diazotroph-specific differentially expressed genes (DEGs) and metabolic pathways of plant–diazotroph-specific transcripts, viz., chitinase, brassinosteroid, auxin, Myeloblastosis (MYB), nodulin, and nitrate transporter (NRT), were common in all plant–diazotroph combinations; three transcripts, viz., nitrate transport accessory protein (NAR), thaumatin, and thionin, were exclusive in rice and another three transcripts, viz., no apical meristem, Petunia (NAC), abscisic acid (ABA), and ammonium transporter, were exclusive in soybean. Differential expression of these transcripts and reduction in pathogenesis-related (PR) protein expression show the early interaction. Based on the interaction, it can be inferred that the compatibility of rice and soybean is more with GAB and BRH, respectively. We propose that rice is unable to identify the diazotroph as a beneficial microorganism or a pathogen from an early response. So, it expressed the hypersensitivity-related transcripts along with PR proteins. The molecular mechanism of diazotrophic associations of GAB and BRH with rice vis-à-vis soybean will shed light on the basic understanding of host responses to beneficial microorganisms.
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Song, Xin, Chao Fang, Zi-Qiang Yuan, and Feng-Min Li. "Long-Term Growth of Alfalfa Increased Soil Organic Matter Accumulation and Nutrient Mineralization in a Semi-Arid Environment." Frontiers in Environmental Science 9 (April 1, 2021). http://dx.doi.org/10.3389/fenvs.2021.649346.
Abstract:
Land use patterns and vegetation coverage in semi-arid areas of the Loess Plateau have undergone great changes due to the implementation of the “Grain for Green” project. The introduction of legume pasture species, such as alfalfa (Medicago sativa L.) and sweet clover (Melilotus officinalis L.), is one of the most efficient methods of vegetation restoration and reconstruction in this region. However, there is a need for an effective assessment of the root system distribution and its interaction with soil after long-term introduction. An experiment involving the introduction of alfalfa and sweet clover on abandoned farmlands was initiated in 2003 to assess the long-term effects. After 17 years, root and soil samples at depths of 0–20 and 20–60 cm were collected to characterize the root biomass, root carbon (C), nitrogen (N), and phosphorus (P), soil microbial biomass carbon (MBC) and nitrogen (MBN), soil organic carbon (SOC), and soil N and P. The results showed that the root biomass density of alfalfa in the 0–20 and 20–60 cm layers (63.72 and 12.27 kg m–3, respectively) were significantly higher than for sweet clover (37.43 and 8.97 kg m–3, respectively) and under natural abandonment (38.92 and 9.73 kg m–3, respectively). The SOC, total nitrogen (TN), total phosphorus (TP), available phosphorus (AP), nitrate-nitrogen (NO3–-N), MBC and MBN in the 0–20 and 20–60 cm layers were higher after alfalfa introduction compared with sweet clover introduction and natural abandonment, although the ammonia-nitrogen (NH4+-N) concentration in the 0–20 cm layer was lower. There were significantly positive correlations between root biomass density and both soil nutrients and microbial biomass, while there was a negative correlation between the soil NH4+-N and root biomass density. These results indicate that alfalfa root growth improved soil organic matter accumulation and nutrient mineralization. The accumulation and mineralization of soil nutrients also guaranteed root and microorganism growth. Therefore, it was concluded that alfalfa introduction will promote soil nutrients immobilization and mineralization and may enable sustainable land use in the semi-arid region of the Loess Plateau, China.
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Massot, Francisco, Panagiotis Gkorezis, Jonathan Van Hamme, Damian Marino, Bojana Spirovic Trifunovic, Gorica Vukovic, Jan d’Haen, et al. "Isolation, Biochemical and Genomic Characterization of Glyphosate Tolerant Bacteria to Perform Microbe-Assisted Phytoremediation." Frontiers in Microbiology 11 (January 14, 2021). http://dx.doi.org/10.3389/fmicb.2020.598507.
Abstract:
The large-scale use of the herbicide glyphosate leads to growing ecotoxicological and human health concerns. Microbe-assisted phytoremediation arises as a good option to remove, contain, or degrade glyphosate from soils and waterbodies, and thus avoid further spreading to non-target areas. To achieve this, availability of plant-colonizing, glyphosate-tolerant and -degrading strains is required and at the same time, it must be linked to plant-microorganism interaction studies focusing on a substantive ability to colonize the roots and degrade or transform the herbicide. In this work, we isolated bacteria from a chronically glyphosate-exposed site in Argentina, evaluated their glyphosate tolerance using the minimum inhibitory concentration assay, their in vitro degradation potential, their plant growth-promotion traits, and performed whole genome sequencing to gain insight into the application of a phytoremediation strategy to remediate glyphosate contaminated agronomic soils. Twenty-four soil and root-associated bacterial strains were isolated. Sixteen could grow using glyphosate as the sole source of phosphorous. As shown in MIC assay, some strains tolerated up to 10000 mg kg–1 of glyphosate. Most of them also demonstrated a diverse spectrum of in vitro plant growth-promotion traits, confirmed in their genome sequences. Two representative isolates were studied for their root colonization. An isolate of Ochrobactrum haematophilum exhibited different colonization patterns in the rhizoplane compared to an isolate of Rhizobium sp. Both strains were able to metabolize almost 50% of the original glyphosate concentration of 50 mg l–1 in 9 days. In a microcosms experiment with Lotus corniculatus L, O. haematophilum performed better than Rhizobium, with 97% of glyphosate transformed after 20 days. The results suggest that L. corniculatus in combination with to O. haematophilum can be adopted for phytoremediation of glyphosate on agricultural soils. An effective strategy is presented of linking the experimental data from the isolation of tolerant bacteria with performing plant-bacteria interaction tests to demonstrate positive effects on the removal of glyphosate from soils.
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Becerril-Espinosa, Amayaly, Rosalba M. Hernández-Herrera, Ivan D. Meza-Canales, Rodrigo Perez-Ramirez, Fabián A. Rodríguez-Zaragoza, Lucila Méndez-Morán, Carla V. Sánchez-Hernández, et al. "Habitat-adapted heterologous symbiont Salinispora arenicola promotes growth and alleviates salt stress in tomato crop plants." Frontiers in Plant Science 13 (August 8, 2022). http://dx.doi.org/10.3389/fpls.2022.920881.
Abstract:
To ensure food security given the current scenario of climate change and the accompanying ecological repercussions, it is essential to search for new technologies and tools for agricultural production. Microorganism-based biostimulants are recognized as sustainable alternatives to traditional agrochemicals to enhance and protect agricultural production. Marine actinobacteria are a well-known source of novel compounds for biotechnological uses. In addition, former studies have suggested that coral symbiont actinobacteria may support co-symbiotic photosynthetic growth and tolerance and increase the probability of corals surviving abiotic stress. We have previously shown that this activity may also hold in terrestrial plants, at least for the actinobacteria Salinispora arenicola during induced heterologous symbiosis with a wild Solanaceae plant Nicotiana attenuata under in vitro conditions. Here, we further explore the heterologous symbiotic association, germination, growth promotion, and stress relieving activity of S. arenicola in tomato plants under agricultural conditions and dig into the possible associated mechanisms. Tomato plants were grown under normal and saline conditions, and germination, bacteria-root system interactions, plant growth, photosynthetic performance, and the expression of salt stress response genes were analyzed. We found an endophytic interaction between S. arenicola and tomato plants, which promotes germination and shoot and root growth under saline or non-saline conditions. Accordingly, photosynthetic and respective photoprotective performance was enhanced in line with the induced increase in photosynthetic pigments. This was further supported by the overexpression of thermal energy dissipation, which fine-tunes energy use efficiency and may prevent the formation of reactive oxygen species in the chloroplast. Furthermore, gene expression analyses suggested that a selective transport channel gene, SlHKT1,2, induced by S. arenicola may assist in relieving salt stress in tomato plants. The fine regulation of photosynthetic and photoprotective responses, as well as the inhibition of the formation of ROS molecules, seems to be related to the induced down-regulation of other salt stress response genes, such as SlDR1A-related genes or SlAOX1b. Our results demonstrate that the marine microbial symbiont S. arenicola establishes heterologous symbiosis in crop plants, promotes growth, and confers saline stress tolerance. Thus, these results open opportunities to further explore the vast array of marine microbes to enhance crop tolerance and food production under the current climate change scenario.
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Shawan, Mohammad Mahfuz Ali Khan, Sajal Kumar Halder, and Md Ashraful Hasan. "Luteolin and abyssinone II as potential inhibitors of SARS-CoV-2: an in silico molecular modeling approach in battling the COVID-19 outbreak." Bulletin of the National Research Centre 45, no. 1 (January 20, 2021). http://dx.doi.org/10.1186/s42269-020-00479-6.
Abstract:
Abstract Background At present, the entire world is in a war against COVID-19 pandemic which has gradually led us toward a more compromised “new normal” life. SARS-CoV-2, the pathogenic microorganism liable for the recent COVID-19 outbreak, is extremely contagious in nature resulting in an unusual number of infections and death globally. The lack of clinically proven therapeutic intervention for COVID-19 has dragged the world’s healthcare system into the biggest challenge. Therefore, development of an efficient treatment scheme is now in great demand. Screening of different biologically active plant-based natural compounds could be a useful strategy for combating this pandemic. In the present research, a collection of 43 flavonoids of 7 different classes with previously recorded antiviral activity was evaluated via computational and bioinformatics tools for their impeding capacity against SARS-CoV-2. In silico drug likeness, pharmacophore and Absorption, Distribution, Metabolism, Excretion and Toxicity (ADMET) profile analysis of the finest ligands were carried out using DataWarrior, DruLiTo and admetSAR programs, respectively. Molecular docking was executed by AutoDock Vina, while molecular dynamics simulation of the target protein–ligand bound complexes was done using nanoscalable molecular dynamics and visual molecular dynamics software package. Finally, the molecular target analysis of the selected ligands within Homo sapiens was conducted with SwissTargetPredcition web server. Results Out of the forty-three flavonoids, luteolin and abyssinone II were found to develop successful docked complex within the binding sites of target proteins in terms of lowest binding free energy and inhibition constant. The root mean square deviation and root mean square fluctuation values of the docked complex displayed stable interaction and efficient binding between the ligands and target proteins. Both of the flavonoids were found to be safe for human use and possessed good drug likeness properties and target accuracy. Conclusions Conclusively, the current study proposes that luteolin and abyssinone II might act as potential therapeutic candidates for SARS-CoV-2 infection. In vivo and in vitro experiments, however, should be taken under consideration to determine the efficiency and to demonstrate the mechanism of action.
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Dutta, Tushar K., and Victor Phani. "The pervasive impact of global climate change on plant-nematode interaction continuum." Frontiers in Plant Science 14 (April 6, 2023). http://dx.doi.org/10.3389/fpls.2023.1143889.
Abstract:
Pest profiles in today’s global food production system are continually affected by climate change and extreme weather. Under varying climatic conditions, plant-parasitic nematodes (PPNs) cause substantial economic damage to a wide variety of agricultural and horticultural commodities. In parallel, their herbivory also accredit to diverse ecosystem services such as nutrient cycling, allocation and turnover of plant biomass, shaping of vegetation community, and alteration of rhizospheric microorganism consortium by modifying the root exudation pattern. Thus PPNs, together with the vast majority of free-living nematodes, act as ecological drivers. Because of direct exposure to the open environment, PPN biology and physiology are largely governed by environmental factors including temperature, precipitation, humidity, atmospheric and soil carbon dioxide level, and weather extremes. The negative effects of climate change such as global warming, elevated CO2, altered precipitation and the weather extremes including heat waves, droughts, floods, wildfires and storms greatly influence the biogeographic range, distribution, abundance, survival, fitness, reproduction, and parasitic potential of the PPNs. Changes in these biological and ecological parameters associated to the PPNs exert huge impact on agriculture. Yet, depending on how adaptable the species are according to their geo-spatial distribution, the consequences of climate change include both positive and negative effects on the PPN communities. While assorting the effects of climate change as a whole, it can be estimated that the changing environmental factors, on one hand, will aggravate the PPN damage by aiding to abundance, distribution, reproduction, generation, plant growth and reduced plant defense, but the phenomena like sex reversal, entering cryptobiosis, and reduced survival should act in counter direction. This seemingly creates a contraposition effect, where assessing any confluent trend is difficult. However, as the climate change effects will differ according to space and time it is apprehensible that the PPNs will react and adapt according to their location and species specificity. Nevertheless, the bio-ecological shifts in the PPNs will necessitate tweaking their management practices from the agri-horticultural perspective. In this regard, we must aim for a ‘climate-smart’ package that will take care of the food production, pest prevention and environment protection. Integrated nematode management involving precise monitoring and modeling-based studies of population dynamics in relation to climatic fluctuations with escalated reliance on biocontrol, host resistance, and other safer approaches like crop rotation, crop scheduling, cover cropping, biofumigation, use of farmyard manure (FYM) would surely prove to be viable options. Although the novel nematicidal molecules are target-specific and relatively less harmful to the environment, their application should not be promoted following the global aim to reduce pesticide usage in future agriculture. Thus, having a reliable risk assessment with scenario planning, the adaptive management strategies must be designed to cope with the impending situation and satisfy the farmers’ need.
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Ayuso-Calles, Miguel, José David Flores-Félix, Filipa Amaro, Ignacio García-Estévez, Alejandro Jiménez-Gómez, Paula Guedes de Pinho, M. Teresa Escribano-Bailón, and Raúl Rivas. "Effect of Rhizobium mechanisms in improving tolerance to saline stress in lettuce plants." Chemical and Biological Technologies in Agriculture 10, no. 1 (September 12, 2023). http://dx.doi.org/10.1186/s40538-023-00463-y.
Abstract:
Abstract Background Soils affected by salinity are a recurring problem that is continually increasing due to the impact of climate change on weather conditions and ineffective agricultural management practices. The use of plant growth promoting (PGP) Bacteria can alleviate its effects. In this regard, the genus Rhizobium has demonstrated excellent PGP capabilities through various plant growth promotion mechanisms and may therefore be a promising biofortifier under saline conditions. However, little is known about the production of volatile organic compounds (VOCs) by bacteria of this genus and their effects on plant development. Here, we aim to characterize the volatilome (the set of volatile metabolites synthesized by an organism) of Rhizobium for the first time and to further investigate the direct and VOC-mediated interaction between a strain of this genus and lettuce, a crop severely affected by salinity, both under saline and non-saline conditions. Results In this study, it was shown that the use of Rhizobium sp. GPTR29 was able to increase the production of lettuce (Lactuca sativa L.) under normal and saline conditions. We analyzed the Rhizobium volatilome under non-saline (0 mM NaCl) and saline (100 mM NaCl) conditions by HS-SPME-GC‒MS and found a differential composition in response to salinity. We detected 20 different compounds, where 3-methyl-1-butanol, 2-methyl-1-butanol, and α-pinene were the backbone of the Rhizobium volatilome. Exposure to these compounds in bicameral plates under salt stress resulted in increases in plant development of 17.1%, 16.0% and 33.1% in aerial part size, number of leaves and root length, respectively. Under greenhouse conditions and salinity, the inoculation of Rhizobium sp. GPTR29 resulted in an increase of 17.8% and 27.4% in shoot fresh and dry weight, respectively. Phenolic compounds were analyzed by HPLC–DAD-MS, revealing an increase in total flavonoid content under salinity conditions (100 mM NaCl) and apigenin derivative, luteolin 7-O-glucoside and quercetin 3-O-glucuronide individually. Conclusions These results provide new avenues for the study of PGP mechanisms in this bacterial genus, such as VOCs and their effects on plant growth, which play an important role in mediating plant–microorganism interactions. Graphical abstract
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Qu, Yunke, Jie Tang, Ben Liu, Hang Lyu, Yucong Duan, Yao Yang, Sining Wang, and Zhaoyang Li. "Rhizosphere enzyme activities and microorganisms drive the transformation of organic and inorganic carbon in saline–alkali soil region." Scientific Reports 12, no. 1 (January 25, 2022). http://dx.doi.org/10.1038/s41598-022-05218-7.
Abstract:
AbstractWestern Jilin Province is one of the world's three major saline–alkali land distribution areas, and is also an important area of global climate change and carbon cycle research. Rhizosphere soil microorganisms and enzymes are the most active components in soil, which are closely related to soil carbon cycle and can reflect soil organic carbon (SOC) dynamics sensitively. Soil inorganic carbon (SIC) is the main existing form of soil carbon pool in arid saline–alkali land, and its quantity distribution affects the pattern of soil carbon accumulation and storage. Previous studies mostly focus on SOC, and pay little attention to SIC. Illumina Miseq high-throughput sequencing technology was used to reveal the changes of community structure in three maize fields (M1, M2 and M3) and three rice fields (R1, R2 and R3), which were affected by different levels of salinization during soil development. It is a new research topic of soil carbon cycle in saline–alkali soil region to investigate the effects of soil microorganisms and soil enzymes on the transformation of SOC and SIC in the rhizosphere. The results showed that the root—soil—microorganism interaction was changed by saline–alkali stress. The activities of catalase, invertase, amylase and β-glucosidase decreased with increasing salinity. At the phylum level, most bacterial abundance decreases with increasing salinity. However, the relative abundance of Proteobacteria and Firmicutes in maize field and Firmicutes, Proteobacteria and Nitrospirae in rice field increased sharply under saline–alkali stress. The results of redundancy analysis showed that the differences of rhizosphere soil between the three maize and three rice fields were mainly affected by ESP, pH and soil salt content. In saline–alkali soil region, β-glucosidase activity and amylase were significantly positively correlated with SOC content in maize fields, while catalase and β-glucosidase were significantly positively correlated with SOC content in rice fields. Actinobacteria, Bacteroidetes and Verrucomicrobia had significant positive effects on SOC content of maize and rice fields. Proteobacteria, Gemmatimonadetes and Nitrospirae were positively correlated with SIC content. These enzymes and microorganisms are beneficial to soil carbon sequestration in saline–alkali soils.
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Behr, Jan Helge, Theresa Kuhl-Nagel, Loreen Sommermann, Narges Moradtalab, Soumitra Paul Chowdhury, Michael Schloter, Saskia Windisch, et al. "Long-term conservation tillage with reduced nitrogen fertilization intensity can improve winter wheat health via positive plant-microorganism feedback in the rhizosphere." FEMS Microbiology Ecology, January 15, 2024. http://dx.doi.org/10.1093/femsec/fiae003.
Abstract:
Abstract Microbiome-based solutions are regarded key for sustainable agroecosystems. However, it is unclear how agricultural practices affect the rhizosphere microbiome, plant-microorganism interactions and crop performance under field conditions. Therefore, we installed root observation windows in a winter wheat field cultivated either under long-term mouldboard plough (MP) or cultivator tillage (CT). Each tillage practice was also compared at two nitrogen (N) fertilization intensities, intensive (recommended N-supply with pesticides/growth regulators) or extensive (reduced N-supply, no fungicides/growth regulators). Shoot biomass, root exudates, leaf metabolites and gene expression were analyzed together with the rhizosphere microbiome (bacterial/archaeal 16S rRNA gene, fungal ITS amplicon and shotgun metagenome sequencing) shortly before flowering. Compared to MP, the rhizosphere of CT winter wheat contained more primary and secondary metabolites, especially benzoxazinoid derivatives. Potential copiotrophic and plant-beneficial taxa (e.g. Bacillus, Devosia, Trichoderma) as well as functional genes (e.g. siderophore production, trehalose synthase, ACC deaminase) were enriched in the CT rhizosphere, suggesting that tillage affected belowground plant-microorganism interactions. In addition, physiological stress markers were suppressed in CT winter wheat compared to MP. In summary, tillage practice was a major driver of crop performance, root deposits and rhizosphere microbiome interactions, while the N-fertilization intensity was also relevant, but less important.
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Sillo, Fabiano, Giovanni Marino, Elisabetta Franchi, Matthew Haworth, Elisa Zampieri, Ilaria Pietrini, Danilo Fusini, Carmelo Mennone, Mauro Centritto, and Raffaella Balestrini. "Impact of irrigation water deficit on two tomato genotypes grown under open field conditions: From the root-associated microbiota to the stress responses." Italian Journal of Agronomy 17, no. 3 (September 13, 2022). http://dx.doi.org/10.4081/ija.2022.2130.
Abstract:
In the context of the climate change scenario in the Mediterranean, natural root-microorganism associations have an impact on the resilience and productivity of crops, and the exploitation of these interactions represents innovative, cost-effective and sustainable crop adaptation strategies. An open field experiment with two commercial Italian tomato cultivars was performed. The soil bacterial communities associated with the two commercial Italian tomato genotypes were characterized alongside their physiological and molecular responses under wellwatered and moderate water deficit (100% and 75% of crop evapotranspiration) treatments. The two genotypes showed contrasting responses to water deficit, primarily through diverse rhizosphere microbiota recruitment under the two irrigation treatments. Highlights - Two tomato genotypes were studied under water deficit in a pilot field trial. - The two genotypes responded differently to water stress from eco-physiological and transcriptomic points of view. - The two genotypes recruited diverse root-associated microbiota, particularly under water deficit.