Dissertations / Theses on the topic 'RNA metabolism'
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Stoppel, Rhea. "Chloroplast RNA metabolism." Diss., lmu, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-152718.
Full textConrad, Heather Miller. "Nuclear and mitochondrial mutations affecting mitochondrial RNA metabolism." Connect to resource, 1987. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1230739011.
Full textWaters, Margaret Fiona. "Enzymes of RNA metabolism in Nostoc sp. MAC." Thesis, University of Liverpool, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.329409.
Full textLOFFREDA, ALESSIA. "RNA Metabolism alteration in amyotrophic lateral sclerosis models." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2014. http://hdl.handle.net/10281/81488.
Full textMiller, Harvey. "The metabolism of tRNAAspargine in the friend erthroleukemia cell /." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=60725.
Full textBird, Gregory A. "Exploring the roles of the RNA Polymerase II CTD in pre-MRNA metabolism /." Connect to full text at ProQuest Digital Dissertations. IP filtered, 2005.
Find full textTypescript. Includes bibliographical references (leaves 130-152). Free to UCDHSC affiliates. Online version available via ProQuest Digital Dissertations;
Goulet, Isabelle. "New Roles for Arginine Methylation in RNA Metabolism and Cancer." Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/20293.
Full textHong, Lingzi. "Act1-Mediated RNA Metabolism in IL-17-Driven Inflammatory Diseases." Case Western Reserve University School of Graduate Studies / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case162673878106271.
Full textSmith, Richard Wilson. "RNA metabolism and the control of protein synthesis in fish." Thesis, University of Aberdeen, 1996. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU089891.
Full textBenbahouche, Nour el Houda. "Investigating the role of extended CBC complexes in RNA metabolism." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS002.
Full textThe cap binding complex (CBC) plays a key role in a number of gene expression pathways and has been proposed to participate in the discrimination of RNA families. It also enhances many RNA processing steps, including transcription, splicing, 3’end formation, degradation, export and translation.Recently, we identified the CBCAP complex, composed of CBC, Ars2 and PHAX. We showed that Ars2 stimulates snRNA 3'-end processing as well as PHAX binding to the CBC, hence coupling snRNA maturation with their export. Other studies showed that the CBC and ARS2 can form another complex that contains ZC3H18-NEXT instead of PHAX. This complex, named CBCN, is a cofactor of the RNA exosome and is involved in the degradation of cryptic RNAs such as PROMPTs and read-through transcripts at histone and snRNA genes. Thus, PHAX and ZC3H18 target specific families of capped RNA toward either export or degradation. Previous studies proposed that PHAX binds specifically to small RNAs and discriminates them over other RNA species. Surprisingly, our CLIP-Seq and RIP-microarrays data showed that in contrast to expectations, PHAX was not specific for snRNAs. It also binds mRNAs as well as other non-coding RNAs and has a weak preference for snRNAs comparing to ZC3H18. To better understand the role of PHAX and ZC3H18, Ifirst determined whether PHAX and ZC3H18 can bind simultaneously to the CBC. Competitive LUMIER IPs indicated that binding of these proteins is mutually exclusive. I then used tethering assays and could show that PHAX and ZC3H18 have opposite effect on mRNA biogenesis. These data go against a model where binding of PHAX or ZC3H18 discriminate RNA families, and instead suggest promiscuous binding for these proteins. In addition, PHAX may exert a positive effect on mRNA processing by preventing binding of ZC3H18 and recruitment of the RNA exosome. Last but not least, our RT-QPCR data show that PHAX and ZC3H18 depletions have functional consequences on the level of mature snRNA, and this is due to a competition between both proteins which occur on those snRNA read-through transcripts.To further explore the role of ZC3H18, I performed a two-hybrid screen and identified several splicing factors. I could validate these interactions, identify the domains involved and show that binding of some of these factors is exclusive with that of NEXT. Importantly, proteomic experiments with one of these factors identified a complex that makes the link between the cap and the splicing machinery. In agreement, RNA-Seq analysis of ZC3H18 knock-down cells showed alterations in splicing of cap-proximal introns, for a small set of genes.Altogether, this work reveals how the multiple roles of the RNA cap are achieved at the biochemical level, and suggests that the nascent RNA sequence triggers formation of one among several mutually exclusive complexes
Cornella, Nicola. "Characterization of the hnRNP RALY in RNA transcription and metabolism." Doctoral thesis, Università degli studi di Trento, 2017. https://hdl.handle.net/11572/369300.
Full textCornella, Nicola. "Characterization of the hnRNP RALY in RNA transcription and metabolism." Doctoral thesis, University of Trento, 2017. http://eprints-phd.biblio.unitn.it/2625/2/Disclaimer_Cornella.pdf.
Full textWhitman, Samantha. "Fragile X Related Protein-1 (FXR1) Regulates RNA Metabolism in Striated Muscle." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/195153.
Full textBeauchamp, Pascal. "The functional role of the RNA-binding protein HuR in the regulation of muscle cell differentiation /." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111586.
Full textGreenhalgh, Duncan Alan. "Effects of 5-fluorouracil on RNA metabolism in human tumour cells." Thesis, University of Leeds, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236315.
Full textNurmohamed, Salima. "Communication between the Escherichia coli RNA degradative machineries and cellular metabolism." Thesis, University of Cambridge, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.611286.
Full textAudano, M. "THE RNA BINDING PROTEIN ZC3H10 COUPLES MITOCHONDRIAL FUNCTION AND IRON METABOLISM." Doctoral thesis, Università degli Studi di Milano, 2017. http://hdl.handle.net/2434/481986.
Full textFigueiredo, Cardoso Tainã. "Analysis of the genetic basis of porcine meat quality and coat color by using genomic and transcriptomic tools." Doctoral thesis, Universitat Autònoma de Barcelona, 2018. http://hdl.handle.net/10803/662617.
Full textThe main objectives of this Thesis were to investigate the genetic basis of fatness in pigs and to identify the genetic factors involved in the establishment of blond vs red pigmentation patterns in Mangalitza pigs by using genomic and transcriptomic tools. In the first study of the Thesis (Chapter 3), we compare the skeletal muscle expression patterns of two groups of Duroc pigs with different growth and fatness profiles (HIGH: high backfat thickness, intramuscular fat, saturated and unsaturated fatty acid content and serum lipids vs LOW: opposite phenotypes). By using a RNA-Seq technology, we identified 96 genes differentially expressed. Several of these genes are related to lipid metabolism (e.g. SLC27A4, SFRP5 and CES1) and the transcription factor PPARG appears to be a key regulator of porcine fatness. We have also observed that very few non-coding RNAs are differentially expressed in these two groups of pigs, suggesting that the non-coding transcriptome has a limited effect on the establishment of the HIGH and LOW phenotypes. In the second study of the Thesis, we demonstrate the differential expression of specific mRNA isoforms of four genes with a known role in obesity (ITGA5, LITAF, TIMP1 and ANXA2) in HIGH vs LOW pigs. The differential expression of these isoforms may have effects on transcript structure as well as on the protein sequence. In the third study, we aimed to investigate the differential expression of mRNA encoding genes in response to food ingestion. This goal has been achieved by comparing the muscle mRNA expression patterns of Duroc sows before feeding (T0) and 5 h. (T1) and 7 h. (T2) after feeding. Besides genes with a well-known role in energy homeostasis (e.g. PFKFB3 and G0S2), we have identified several genes with a plausible but poorly characterized role in metabolism (e.g. MIGA2, SDC4, and CSRNP1). We have also observed that the set of genes differentially expressed before and after feeding is enriched in transcription factors and pathways related to oxidative stress, angiogenesis, and circadian rhythms. Considering these results, in the fourth study we use quantitative RT-qPCR technique to find out how the expression of 8 circadian genes (ARNTL, BHLHE40, CRY2, NPAS2, NR1D1, PER1, PER2 and SIK1) changes in response to food ingestion in five porcine tissues i.e. skeletal muscle, hypothalamus, liver, intestine and dorsal fat. Our results indicate that the expression of the Clock genes does not change in the hypothalamus, the tissue containing the central clock entrained by light, but in contrast, it is strongly modified in the other four tissues. This finding demonstrates that nutrition changes the expression of circadian genes integrated in peripheral clocks. Finally, in the fifth study, we have analysed, in collaboration with researchers of the Research Institute for Animal Breeding and Nutrition (Hungary) and the University of Cluj-Napoca (Romania), the genetic basis of coat color (red vs blond) of Mangalitza pigs. By combining a selection scan and a genome-wide association study, we have found that the SLC45A2 gene is probably involved in the genetic determination of pigmentation in Mangalitza pigs, a result that agrees well with previous studies demonstrating the implication of this locus on the color patterns of multiple mammalian species including humans. More specifically, two missense SNPs c.806G>A (p.Gly269Glu) and c.956G>A (p.Arg319His) in the SLC45A2 locus appear to be strongly but not fully associated with the red and blond coat colors of Mangalitza pigs. This finding suggests the existence of addiitonal genetic factors regulating the pigmentation of Mangalitza pigs.
Ajamian, Lara. "Up-frameshift proteins and their distinct roles in HIV-1 RNA metabolism." Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=116866.
Full textLe VIH-1 requiert plusieurs protéines cellulaires à chaque étape de son cycle de réplication pour assurer une réplication efficace. Notre travail a mené à l'identification que l'ARN génomique du VIH-1 n'est pas un substrat pour la dégradation des ARNm aberrants (NMD), même si elle a plusieurs cadres de lecture ainsi qu'une longue 3'UTR. Nous avons démontré que UPF1 est impliqué dans la stabilité de l'ARN génomique du VIH-1 car la surexpression de UPF1 a engendré une augmentation des niveaux d'ARN du VIH-1 et de Gag. Par ailleurs, le rôle de UPF1 est distinct de son rôle dans le mécanisme NMD, il est observé dans les compartiments nucléaires et cytoplasmiques et ne nécessite pas sa liaison à UPF2. De plus, la fonction navette de UPF1 est requise pour assurer l'exportation de l'ARN génomique du VIH-1 car le mutant NES d'UPF1 bloque son export et le mutant NLS n'immunoprécipite pas avec celui-ci. Ce nouveau rôle d'UPF1 est observé dans la présence et l'absence de Rev. De plus, UPF1 se trouve en complexe avec Rev, CRM1, Nup62 et DDX3, les protéines cellulaires déjà caractérisées comme étant impliquées dans l'exportation de l'ARN génomique du VIH-1. Enfin, nous avons également identifié UPF2 comme étant un régulateur négatif, de telle sorte que sa liaison à UPF1 engendre un blocage nucléaire de l'ARN génomique du VIH-1. Nous avons identifié un mécanisme possible démontrant comment le VIH-1 s'évade du mécanisme NMD en cooptant UPF1 pour faciliter l'exportation, la stabilité et la traduction de l'ARN génomique du VIH-1.
Kew, Chun [Verfasser], and Adam [Gutachter] Antebi. "Control of Innate Immunity by RNA Metabolism / Chun Kew ; Gutachter: Adam Antebi." Köln : Universitäts- und Stadtbibliothek Köln, 2018. http://d-nb.info/1180601556/34.
Full textLeech, M. J. "Studies on the metabolism of poly(A)'+RNA in the imbibing wheat embryo." Thesis, University of Manchester, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383174.
Full textMa, Jing 1978. "The requirement of the DEAD-box protein DDX24 for the packaging of human immunodeficiency virus type 1 RNA /." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=116084.
Full textIn this study, we found that a DEAD-box protein named DDX24 associates with HIV-1 Gag in an RNA-dependent manner but is not found within virus particles. Knockdown of DDX24 inhibits the packaging of HIV-1 RNA and thus diminishes viral infectivity. The decreased viral RNA packaging as a result of DDX24-knockdown is observed only in the context of the Rev/RRE (Rev response element)-dependent but not the CTE (constitutive transport element)-mediated nuclear export of viral RNA, which is explained by the specific interaction of DDX24 with the Rev protein. We propose that DDX24 acts at the early phase of HIV-1 RNA metabolism prior to nuclear export and the consequence of this action extends to the viral RNA packaging stage during virus assembly.
Séguin, Béatrice. "Control of HIV-1 RNA metabolism, the role of splice sites and intron sequences in unspliced viral RNA subcellular distribution." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0002/MQ40793.pdf.
Full textVoss, Jarrod Edwin. "Structural studies of bacterial and macromolecular machines of RNA metabolism and drug transport." Thesis, University of Cambridge, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708564.
Full textGiunta, Michele. "Exosomal protein deficiencies : how abnormal RNA metabolism results in childhood-onset neurological diseases." Thesis, University of Newcastle upon Tyne, 2017. http://hdl.handle.net/10443/3669.
Full textSun, Xin. "Molecular structure and intracellular location of five RNA-associated proteins in the salivary glands of the dipteran Chironomus tentans /." Stockholm, 1998. http://diss.kib.ki.se/1998/91-628-3291-3/.
Full textPrichard, Lisa. "The role of the IQ motif, a protein kinase C and calmodulin regulatory domain, in neuroplasticity, RNA processing, and RNA metabolism /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/6302.
Full textZhang, Tong. "Characterization of the shuttling properties of RNA-binding TIA proteins." Doctoral thesis, Universite Libre de Bruxelles, 2005. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210999.
Full textKafaie, Jafar. "Role of the NC protein of human immunodeficiency virus type 1 in viral RNA dimerization and packaging, as well as in virus replication and stability." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111914.
Full textEser, Philipp [Verfasser], and Patrick [Akademischer Betreuer] Cramer. "Quantitative genome-wide studies of RNA metabolism in yeast / Philipp Eser. Betreuer: Patrick Cramer." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2016. http://d-nb.info/1104128985/34.
Full textStoppel, Rhea [Verfasser], and Jörg [Akademischer Betreuer] Meurer. "Chloroplast RNA metabolism : global players and site specific factors / Rhea Stoppel. Betreuer: Jörg Meurer." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2012. http://d-nb.info/1030475458/34.
Full textKiss, Daniel L. "The Exozyme Model: A New Paradigm of Exosome Subunit Activity Revealed by Diverse and Distinct Substrate Specificities of Exosome Subunits In Vivo." Case Western Reserve University School of Graduate Studies / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=case1263237977.
Full textDantas, Ive Maíra de Carvalho. "Perfil lipídico na leishmaniose visceral em hamster e expressão de mRNA de genes relacionados ao metabolismo liprotéico." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/99/99131/tde-03082015-103941/.
Full textIn the active phase of visceral leishmaniasis (VL) changes occur in lipoprotein me-tabolism with reduction in HDL and increase in triglyceride (TG) levels. From these data, in this project we focused these changes during the progression of the infection and we approached some elements as their underlying factors. Since these changes may result from the reduction of the activity and the expression of the lipoprotein lipase (LPL), of the peroxisome proliferator-activated receptor alpha (PPAR?) and of the cholesteryl ester transfer protein (CETP), their expression were evaluated during VL progression in hamster. In 2 x 107 L. (L.) infantum amastigote-infected hamsters we observed an increase in the triglycerides in hamsters with 55 days (median = 294.0 mg/dL) and 90 days (303.0 mg/dL) of infection compared with controls of 55 days (119.0 mg/dL) and of 90 days (117.0 mg/dL) (p <= 0.05). The total cholesterol and the HDL levels did not present significant differences between control and in-fected groups at 30, 55 and 90 days of infection. The expression of mRNA of the PPAR in the liver with 55 and 90 days of infection tended to be reduced in infected animals. However the relative expression (CT) of CETP in the liver of hamsters with 55 days of infection was signicantly reduced in infected (0.08) compared with control animals (1.69) (p <= 0.05). The relative expression (CT) of LPL in the heart of hamsters with 90 days of infection was also reduced (1.43) in relation to controls (2.61) (p <= 0.05). There are data in the literature suggesting the importance of lipids for the development of amastigotes in vertebrate host and it is possible that the changes in the lipoprotein levels contribute for the infection progression. Therefore, we evaluated in this study the effect of the lipid-lowering drug ciprofibrate in the control of parasitism in VL in the hamster, knowing that ciprofibrate acts increasing the expression of the PPAR? and of the LPL production and activity. The treatment with ciprofibrate in infected hamsters at 55 days lead to the reduction of triglyceride level (123.0 mg/dL) in relation to non-treated infected animals (294.0 g/dL) (p <= 0.05). Further the triglyceride levels in the non-treated infected animals were in-creased when compared with untreated controls (119.0 mg/dL) (p <= 0.05). There was also reduction of triglyceride in ciprofibrate treated-non infected animals (89.0 mg/dL) compared with non-treated infected animals (p <= 0.05). The cholesterol lev-els were reduced in the ciprofibrate-treated non-infected hamsters (53.5 mg/dL) in comparison to the non-treated infected ones (93.0 mg/dL) (p <= 0.05). In the ciprofibrate-treated infected ones we found a reduction of cholesterol level (53.5 mg/dL) when compared with non treated infected animals (p <= 0.05). The HDL lev-els did not increase with ciprofibrate and they were similar between the non-treated infected hamsters and non-treated controls. The parasite load in the spleen and liver were not reduced with ciprofibrate. In the visceral leishmaniasis in hamster changes occur in the lipid metabolism with increase in the triglyceride level and the reduction of expression of mRNA of LPL and CETP. The treatment with ciprofibrate was ef-fective in the control of changes in the lipoprotein levels.
Jenkins, Kristin Perth 1965. "Identification and partial characterization of two chloroplast-encoded potential RNA metabolism genes roaA and mat1." Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/282114.
Full textCESENA, DANIELE. "The RNA processing proteins Xrn1 and Rrp6 regulate DNA damage checkpoint activation and telomere metabolism." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2017. http://hdl.handle.net/10281/158272.
Full textGenome instability is one of the most pervasive characteristics of cancer cells. It can be due to DNA repair defects, failure to arrest the cell cycle and loss of telomere-end protection that lead to end-to-end fusion and degradation. Among the many types of DNA damage, the DNA Double Strand Break (DSB) is one of the most severe, because it can cause mutations and chromosomal rearrangements. Eukaryotic cells respond to DSBs by activating a checkpoint that depends on the protein kinases Tel1/ATM and Mec1/ATR, in order to arrest the cell cycle until DSBs are repaired. Mec1/ATR is activated by RPA-coated single-stranded DNA (ssDNA) that arises upon nucleolytic degradation (resection) of the DSB. A similar checkpoint response is triggered when the natural ends of eukaryotic chromosomes lose their protection, resembling and being recognized as DSBs. This protection is provided by specialized nucleoprotein complexes called telomeres. Telomeric DNA consists of repetitive G-rich sequences that terminate with a 3’-ended single-stranded overhang (G-tail), which is important for telomere extension by telomerase. Several proteins, including the CST complex, are necessary to maintain telomere structure and length in both yeast and mammals. Emerging evidences indicate that RNA processing proteins play critical, yet poorly understood, roles in genomic stability and telomere metabolism. We provide evidence that the Saccharomyces cerevisiae RNA decay factors Xrn1, Rrp6 and Trf4 facilitate activation of Mec1/ATR by promoting the generation of RPA-coated ssDNA at intrachromosomal DSBs. Xrn1 and Rrp6 are also required to activate a Mec1/ATR-dependent checkpoint at uncapped telomeres due to loss of the CST component Cdc13. Xrn1 promotes checkpoint activation by facilitating the generation of ssDNA at both DSBs and uncapped telomeres. Xrn1 exerts this function at DSBs by promoting the loading of the MRX complex, whereas how it does at uncapped telomeres remains to be determined. By contrast, DSB resection is not affected by the absence of Rrp6 or Trf4, but their lack impairs the recruitment of RPA, and therefore of Mec1, to the DSB. Rrp6 and Trf4 inactivation affects neither Rad51/Rad52 association nor DSB repair by homologous recombination (HR), suggesting that full Mec1 activation requires higher amount of RPA-coated ssDNA than HR-mediated repair. Finally, we demonstrate that Xrn1 maintains telomere length by promoting the association of Cdc13 to telomeres independently of ssDNA generation and exerts this function by downregulating the RIF1 transcript. Our results provide novel links between RNA processing and genome stability.
Liang, Chao. "Aptamer-functionalized lipid nanoparticles targeting osteoblasts as a novel RNA Interference-based bone anabolic strategy." HKBU Institutional Repository, 2016. https://repository.hkbu.edu.hk/etd_oa/325.
Full textBeick, Susanne. "Funktion und Evolution chloroplastidärer PPR-Proteine." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16343.
Full textPPR proteins are the largest family of RNA binding proteins in plants and the vast majority of them is localized to mitochondria or chloroplasts, where they are major players in the RNA metabolism of defined transcripts (Lurin et al., 2004). However, the mechanistic function of these proteins is still not clear. In this study, the plastid PPR protein PPR5, whose ortholog in Arabidopsis thaliana is embryo-essential (Cushing et al., 2005), was functionally characterized in Zea mays. By PPR5 immunoprecipitation and analyses of the coimmunoprecipitated RNA, a specific association to the unspliced tRNA glycine (UCC) was shown in vivo. The analysis of ppr5 maize mutants demonstrated a loss of stability of the tRNA precursor in mutants. It was concluded that the interaction with PPR5 protects the unspliced tRNA from endonucleolytic decay. In addition, close relatives of PPR5 were identified in maize (PPR2, PPR50, and PPR51) by phylogenetic means and maize mutants were isolated. A future characterization of four paralogous PPR proteins might answer whether closely related PPR proteins have similar functions or RNA targets. The analysis of PPR54 in maize demonstrated that PPR5 is needed for the splicing of the ndhA intron in maize as it is in Arabidopsis (Tillich, not published). Three important conclusions concerning the function of PPR proteins in general were drawn from the studies of chosen PPR proteins presented here. First, plastid PPR proteins that are essential in embryo development in eudicots like Arabidopsis should be necessary for plastid translation in most cases. Second, the characterization of PPR5 revealed a possibly ancient functional mechanism of PPR proteins which does not invoke the recruitment of additional catalytic factors but relies on the passive binding of RNA elements. Last, the conservation of function of orthologous PPR proteins in monocots and eudicots, which was shown in the case of PPR54, was demonstrated experimentally for the first time.
Silva, Cátia Cláudia Bárria da. "The role of ribonuclease R in bacterial adaptation to cold shock." Master's thesis, Faculdade de Ciências Médicas, 2011. http://hdl.handle.net/10362/6634.
Full textBorisova, Marina E. [Verfasser]. "p38-MK2 signaling axis regulates RNA metabolism after UV-light-induced DNA damage / Marina E. Borisova." Mainz : Universitätsbibliothek Mainz, 2019. http://d-nb.info/1178732967/34.
Full textMakwana, Kuldeep Makwana. "CIRCADIAN MECHANISMS OF CALORIE RESTRICTION IN DELAYING AGING." Cleveland State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=csu1543844626233009.
Full textGandhi, Minakshi [Verfasser], and Peter [Akademischer Betreuer] Angel. "Role of long non-coding RNA lincNMR in nucleotide metabolism in cancer / Minakshi Gandhi ; Betreuer: Peter Angel." Heidelberg : Universitätsbibliothek Heidelberg, 2019. http://d-nb.info/1195143826/34.
Full textLaberge, MacDonald Tammy. "Molecular Aspects of Nitrogen Metabolism in Fishes." Scholarly Repository, 2009. http://scholarlyrepository.miami.edu/oa_dissertations/668.
Full textPfennig, Juliane [Verfasser], Tomas [Akademischer Betreuer] Pieler, and Michael [Akademischer Betreuer] Kessel. "XDazl function in RNA metabolism in Xenopus laevis / Juliane Pfennig. Betreuer: Tomas Pieler. Gutachter: Tomas Pieler ; Michael Kessel." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://d-nb.info/1079384626/34.
Full textStirling, Susan Renee, and n/a. "The Roles of RasGAP SH3 Domain Binding Proteins (G3BPs) in RNA Metabolism, the Cellular Stress Response and Tumorigenesis." Griffith University. School of Biomolecular and Biomedical Science, 2006. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20070705.175459.
Full textStirling, Susan Renee. "The Roles of RasGAP SH3 Domain Binding Proteins (G3BPs) in RNA Metabolism, the Cellular Stress Response and Tumorigenesis." Thesis, Griffith University, 2006. http://hdl.handle.net/10072/366889.
Full textThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Biomedical Sciences
Full Text
Boone, Lindsey R. "Thyroid Hormone Regulation of Cholesterol Metabolism." [Tampa, Fla] : University of South Florida, 2009. http://purl.fcla.edu/usf/dc/et/SFE0003089.
Full textZanardi, Geila Maria. "Detecção da expressão gênica de enzimas da gliconeogênese na placenta de fêmeas bovinas." Universidade do Estado de Santa Catarina, 2008. http://tede.udesc.br/handle/handle/810.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
A singular feature during mammalian development is the provision of nutrients from the maternal system to the conceptus through the placenta. To the fetus, the placenta consists of a multi-functional organ with an intense metabolism, characterized in an adult as separated functions in multiple organs. This study aimed to verify the presence of key enzymes in the bovine placenta responsible for glyconeogenesis, which is a metabolic pathway used for Dglucose synthesis from non-carbohydrate compounds. Such pathway is common to the liver metabolism used to maintain glucose homeostasis in plasma, especially during fasting or postprandial absorption. No studies on the occurrence of glyconeogenesis in the bovine placenta have been reported thus far. In this study, a qualitative gene expression analysis was carried out for three key glyconeogenic enzymes: fructose-1,6-bisphosphatase (F1,6B), glucose-6- phosphatase (G6-P) e phosphoenolpyruvate carboxikinase (PEP-CK) in the bovine placental tissue at three distinct gestation periods. For that, placentome fragments from 5 pregnant females from each period were used for the detection of transcripts for the three enzymes above by reverse transcription-polymerase chain reaction (RT-PCR). Amplified PCR products obtained for each enzyme from the placenta and adult liver (positive control) were analyzed in 1% agarose gel, followed by purification, DNA cloning e sequencing for confirmation of amplification specificity. The RT-PCR analysis followed by DNA sequencing revealed the presence of all three glyconeogenic enzymes in the bovine placenta from all three gestational periods. Based on our results, the glyconeogenic pathway may occur in the placental tissue in cattle during pregnancy. However, more studies are still needed for the confirmation of the occurrence and temporal importance of the glyconeogenesis in the bovine placenta
Uma característica singular do desenvolvimento dos mamíferos é a provisão de nutrientes do organismo materno por intermédio da placenta. Para o feto, a placenta consiste na combinação, em apenas um órgão, de muitas atividades funcionais, que no adulto são separadas e, além disso, possui um intenso metabolismo. Este trabalho teve como objetivo verificar a expressão de enzimas chave na placenta bovina para a gliconeogênese, que consiste em uma via metabólica capaz de sintetizar D-glicose, utilizando-se compostos que não são carboidratos. Essa via se processa no fígado e é utilizada pelo organismo para manter os níveis glicêmicos normais em condições de jejum e de pós-absorsão. Atualmente não existem estudos sobre a gliconeogênese na placenta bovina. Neste estudo foi avaliada a expressão gênica na placenta bovina de três enzimas gliconeogênicas: frutose-1,6-bisfosfatase (F1,6B), glicose-6-fosfatase (G6-P) e fosfoenolpiruvato carboxiquinase (PEP-CK) nos três trimestres gestacionais. Para tanto, foram utilizados fragmentos de placentônios de 5 fêmeas bovinas para cada terço de gestação para a avaliação da expressão gênica das três enzimas gliconeogênicas após a transcripção reversa e reação em cadeia da polimerase (RT-PCR). Os produtos de PCR amplificados obtidos para cada enzima na placenta bem como para o fígado de animal adulto, que foi utilizado como controle positivo, foram analisados em gel de agarose a 1%, purificados, clonados e seqüenciados para a confirmação da especificidade de amplificação. A análise da expressão gênica através do RT-PCR e o sequenciamento demonstrou a presença de mRNA para as três enzimas gliconeogênicas na placenta bovina nos três períodos gestacionais. Baseado em nossos resultados, é possível que a rota gliconeogênica ocorra no tecido placentário de bovinos durante a gestação. Porém, ainda são necessários mais estudos para a confirmação da ocorrência e importância temporal da gliconeogênese na placenta bovina
Presnyak, Vladimir. "Effects of Codon Usage on mRNA Translation and Decay." Case Western Reserve University School of Graduate Studies / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1427387336.
Full textGkatza, Nikoletta A. "RNA modifications and processing in cell homeostasis and in response to oxidative stress." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/277276.
Full textCarvalho, Ana Elisa Teófilo Saturi de. "Metabolismo energético mitocondrial e cardiomiogênese para regeneração cardíaca." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/5/5131/tde-25102016-161531/.
Full textDespite advances in recent years, the replacement of cardiomyocytes remains one of the biggest challenges in regenerative medicine. The existence of endogenous mechanisms of cardiac proliferation prompted us to seek the understanding of molecular events involved in cardiomyocyte proliferation in postnatal life. In this study, we investigated the influence of mitochondrial energy metabolism in cardiomyogenesis, and its impact on cardiac regeneration. At first, it was described for the first time the model of heart apical resection in neonatal rats, where there is a limited period the first 24 hours of life that animal is able to regenerate cardiac tissue, forming new cardiomyocytes and allowing the maintenance cardiac function in adulthood. This ability is lost seven days after birth, when repair is basically by fibrotic tissue and consequent impairment for heart function. Interestingly, data showed hypoperfusion of the apical region in both resected animals, which possibly resulted in mitochondrial damage in adulthood without affecting heart function. In order to investigate the molecular events of neonatal cardiac regeneration was performed RNA sequencing of hearts from newborn rats with 1 and 7 days of life, resected and sham, which pointed out the importance of age in the different expression of genes related to metabolism, and the intervention of resection had little influence on this. The results showed exchange of expression of enzymes isoforms from glycolytic pathway and hyperregulation of genes from beta-oxidation, oxidative phosphorylation and tricarboxylic acid cycle pathways, during postnatal maturation. However, the functional data of the metabolic activity of cardiac tissue and culture of neonatal cardiomyocytes showed that both anaerobic glycolysis and oxygen consumption related to mitochondrial oxidation were higher in 1-day-old newborns, and were reduced with cardiac development. The high rates of oxygen consumption in 1-day-old cardiomyocytes were related mainly to ATP production. These 1-day-old cardiomyocytes were able to proliferate in culture by serum stimulation. Therefore, the analysis of gene expression alone appeared to be a partial indicator of functional state of metabolism. The non-lethal inhibition of oxidative phosphorylation highlighted the importance of mitochondrial metabolism in the proliferative capacity of cardiomyocytes in postnatal life. Data suggest that the first day after birth covers a high energy demand for both terminal differentiation of cardiac cells and last robust phase of cardiomyocyte proliferation in postnatal life, and show the importance of mitochondrial metabolism in the regenerative process