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1

service), SpringerLink (Online, ed. MicroRNA Interference Technologies. Berlin, Heidelberg: Springer-Verlag Berlin Heidelberg, 2009.

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2

Yves, Christen, and SpringerLink (Online service), eds. Macro Roles for MicroRNAs in the Life and Death of Neurons. Berlin, Heidelberg: Springer-Verlag Berlin Heidelberg, 2010.

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3

Slack, Frank J. MicroRNAs in development and cancer. London: Imperial College Press, 2011.

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4

Clouet-d'Orval, Béatrice, ed. RNA Metabolism and Gene Expression in Archaea. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-65795-0.

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5

Koloteva, Nadejda. Regulation of eukaryotic gene expression via RNA-RNA and RNA-protein interactions. Manchester: UMIST, 1997.

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6

Wang, Zhiguo. MicroRNA expression detection methods. Heidelberg: Springer, 2010.

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7

J, Kay, Ballard F. J, and Mayer R. J, eds. Gene expression: Regulation at the RNA and protein levels. London: Biochemical Society, 1989.

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8

F, Gesteland Raymond, and SpringerLink (Online service), eds. Recoding: Expansion of Decoding Rules Enriches Gene Expression. New York, NY: Springer Science+Business Media, LLC, 2010.

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9

NATO/CEC, Advanced Research Workshop on "Post-Transcriptional Control of Gene Expression" (1990 Goslar Germany). Post-transcriptional control of gene expression. Berlin: Springer-Verlag, 1990.

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10

Next-generation MicroRNA expression profiling technology: Methods and protocols. New York: Humana Press, 2012.

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11

RNA worlds: From life's origins to diversity in gene regulation. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory Press, 2011.

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12

Eithan, Galun, ed. RNA silencing: With an appendix on gene therapy by Eithan Galun. Singapore: World Scientific, 2005.

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13

You, Yongping. Targets in gene therapy. Rijeka: InTech, 2011.

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14

Institute of Medicine (U.S.). Meeting, ed. Human gene therapy. Cambridge, Mass: Harvard University Press, 1988.

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15

Non coding RNAs in plants. Heidelberg: Springer, 2011.

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16

Non-coding RNAs and epigenetic regulation of gene expression: Drivers of natural selection. Norfolk, UK: Caister Academic Press, 2012.

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17

Tripp, Ralph A., and S. Mark Tompkins, eds. Roles of Host Gene and Non-coding RNA Expression in Virus Infection. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-030-05369-7.

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18

Magnusson, Lisa. Global regulation of gene expression in Escherichia coli: The role of ppGpp, DksA, and the levels of RNA polymerase. Göteborg: Göteborgs universitet, 2007.

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19

Magnusson, Lisa. Global regulation of gene expression in Escherichia coli: The role of ppGpp, DksA, and the levels of RNA polymerase. Göteborg: Göteborgs universitet, 2007.

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20

Barrett, Lucy W. Untranslated gene regions and other non-coding elements: Regulation of eukaryotic gene expression. Basel: Springer, 2013.

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21

RNA interference: From biology to clinical applications. New York: Springer, 2010.

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22

Antiviral RNAi: Concepts, methods, and applications. New York: Humana Press/Springer Science, 2011.

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23

Erdmann, V. A., and Jan Barciszewski. Non coding RNAs in plants. Heidelberg: Springer, 2011.

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24

RNA therapeutics: Function, design, and delivery. New York: Humana Press, 2010.

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25

RNAi and microRNA-mediated gene regulation in stem cells: Methods, protocols, and applications. New York: Humana Press, 2010.

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26

Rhoads, Robert E. MiRNA regulation of the translational machinery. Edited by SpringerLink (Online service). Berlin: Springer, 2009.

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27

Divan, Aysha, and Janice A. Royds. 3. RNA. Oxford University Press, 2016. http://dx.doi.org/10.1093/actrade/9780198723882.003.0003.

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The first RNA molecules to be discovered were those involved in protein synthesis, mRNA, transfer RNA (tRNA), and ribosomal RNA (rRNA). In recent years, a vast number of additional RNA molecules have been identified. ‘RNA’ explains that these are non-coding RNAs that are not involved in protein synthesis, but influence many normal cellular and disease processes by regulating gene expression. RNA interference (RNAi) as one of the main ways in which gene expression is regulated is described with applications to therapy. Classes of RNA, including long non-coding RNAs and catalytic RNAs, are explained along with RNA techniques used to study RNA molecule and gene function.
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28

Mouldy, Sioud, ed. Ribozymes and siRNA protocols. 2nd ed. Totowa, N.J: Humana Press, 2004.

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29

Sioud, Mouldy. Ribozymes and siRNA protocols. 2nd ed. Humana Press, 2004.

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30

Suffredini, Anthony F., and J. Perren Cobb. Genetic and molecular expression patterns in critical illness. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199600830.003.0031.

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Investigators who study RNA, proteins, or metabolites use analytic platforms that simultaneously measure changes in the relative abundance of thousands of molecules in a single biological sample. Over the last decade, the application of these high-throughput, genome-wide platforms to study critical illness and injury has generated huge quantities of data that require specialized computational skills for analysis. These investigations hold promise for improving our understanding of the host response, thereby transforming the practice of intensive care. This chapter summarizes recent technological and computational approaches used in genomics, proteomics, and metabolomics. While major advances have been made with these approaches when applied to chronic diseases, the acute nature of critical illness and injury has unique challenges. The rapidity of initiating events, the trajectory of inflammation that follows injury or infection and the interplay of host responses to a replicating infection, all have major effects on changes in gene and molecular expression. This complexity is further accentuated by measurement that may vary with the timing and type of tissue sampled after the critical event. In addition, the hunt for novel molecular markers holds promise for identifying patients at risk for severe illness and for enabling more individualized therapy.
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31

McCarthy, John E. G. Post-Transcriptional Control of Gene Expression ). Springer, 2011.

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32

Clouet-d'Orval, Béatrice. RNA Metabolism and Gene Expression in Archaea. Springer, 2017.

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33

Takeo, Takeyama, ed. Messenger RNA research perspectives. New York: Nova Science Publishers, 2008.

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34

1961-, Gaur Rajesh K., and Rossi John J, eds. Regulation of gene expression by small RNAs. Boca Raton: Taylor & Francis, 2009.

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35

Rossi, John J., and Rajesh K. Gaur. Regulation of Gene Expression by Small RNAs. Taylor & Francis Group, 2017.

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36

(Editor), Rajesh K. Gaur, and John J. Rossi (Editor), eds. Regulation of Gene Expression by Small RNAs. CRC, 2008.

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37

Rossi, John J., and Rajesh K. Gaur. Regulation of Gene Expression by Small RNAs. Taylor & Francis Group, 2009.

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38

Gene Transfer: Delivery And Expression of DNA And Rna. Cold Spring Harbor Laboratory Press, 2006.

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39

Galun, Eithan, and Esra Galun. RNA Silencing: With an Appendix on Gene Therapy. World Scientific Publishing Company, 2005.

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40

Menzies, Fiona. Maternal RNA stability analyzed using gene expression profiling in Drosophila. 2004.

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41

Laurence, Jeffrey. Translating MicroRNAs to the Clinic. Elsevier Science & Technology Books, 2016.

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42

Decheng, Yang, ed. RNA viruses: Host gene responses to infections. New Jersey: World Scientific, 2009.

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43

H, Murray James A., ed. Antisense RNA and DNA. New York: Wiley-Liss, 1992.

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44

1939-, Erickson Robert P., and Izant Jonathan G, eds. Gene regulation: Biology of antisense RNA and DNA. New York: Raven Press, 1992.

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45

Fisher, Paul B., Carlo Croce, and Kenneth D. Tew. MiRNA and Cancer. Elsevier Science & Technology Books, 2017.

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46

Jeffrey, Wilusz, ed. Post-transcriptional gene regulation. Totowa, N.J: Humana Press, 2008.

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47

Post-Transcriptional Gene Regulation. Humana Press, 2007.

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48

Lehmann, Nielsen Kåre, ed. Serial analysis of gene expression (SAGE): Methods and protocols. Totowa, N.J: Humana Press, 2008.

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49

(Editor), Theodore Friedmann, and John Rossi (Editor), eds. Gene Transfer: Delivery And Expression of DNA And RNA, A Laboratory Manual. Cold Spring Harbor Laboratory Press, 2006.

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50

1935-, Friedmann Theodore, and Rossi John J, eds. Gene transfer: Delivery and expression of DNA and RNA : a laboratory manual. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory Press, 2007.

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