Relevant bibliographies by topics / RNA; Gene expression; Therapy

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'RNA; Gene expression; Therapy'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 February 2022

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Journal articles on the topic "RNA; Gene expression; Therapy"

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Nayak, Ketul, Hiral Panchal, Shivani Patel, and Devam Desai. "SIRNA GENE THERAPY: A REVIEW." International Journal of Advanced Research 8, no. 10 (October 31, 2020): 1121–26. http://dx.doi.org/10.21474/ijar01/11942.

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RNA inference is new concept in gene therapy it acts by expression of specific genes to treat various diseases like cancer. It is also called as RNAi. In presence of dicer enzyme long double stranded RNA (dsRNA) is cut to small interfering RNAs called SiRNAs. SiRNAs are part of RNAi. It is mainly pursued for cancer therapy. It is the new promising agent which can produce specific gene expression and also post transcriptional gene regulation process for disease treatment. In this current review I gathered lots of basic information about SiRNA like its structure, design, delivery system etc in short sweet manner.
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Zhang, Junlong. "Special delivery: Small RNAs silencing gene expression." Biochemist 26, no. 5 (October 1, 2004): 20–23. http://dx.doi.org/10.1042/bio02605020.

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RNA interference (RNAi), which refers to RNA-induced transcriptional gene silencing, is a natural phenomenon that exists widely in living organisms. Recent advances in RNAi research indicate that RNAi technology is a powerful tool in studying gene function and has a great potential in gene therapy. Although many methods, including viral and non-viral vectors, have been used to deliver small interference RNA molecules into cells and animals, development of better delivery methods is still crucial for the application of RNAi technology in both basic research and gene therapy.
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Huang, Wei-Hsuan, Chao-Neng Tseng, Jen-Yang Tang, Cheng-Hong Yang, Shih-Shin Liang, and Hsueh-Wei Chang. "RNA Editing and Drug Discovery for Cancer Therapy." Scientific World Journal 2013 (2013): 1–5. http://dx.doi.org/10.1155/2013/804505.

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RNA editing is vital to provide the RNA and protein complexity to regulate the gene expression. Correct RNA editing maintains the cell function and organism development. Imbalance of the RNA editing machinery may lead to diseases and cancers. Recently, RNA editing has been recognized as a target for drug discovery although few studies targeting RNA editing for disease and cancer therapy were reported in the field of natural products. Therefore, RNA editing may be a potential target for therapeutic natural products. In this review, we provide a literature overview of the biological functions of RNA editing on gene expression, diseases, cancers, and drugs. The bioinformatics resources of RNA editing were also summarized.
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Caplen, N. J. "Gene Therapy Progress and Prospects. Downregulating gene expression: the impact of RNA interference." Gene Therapy 11, no. 16 (August 2004): 1241–48. http://dx.doi.org/10.1038/sj.gt.3302324.

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Lei, Sibei, Xueyan Zhang, Jingmei Li, Yan Gao, Jieping Wu, Xingmei Duan, and Ke Men. "Current Progress in Messenger RNA-Based Gene Therapy." Journal of Biomedical Nanotechnology 16, no. 7 (July 1, 2020): 1018–44. http://dx.doi.org/10.1166/jbn.2020.2961.

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Based on its rapid expression, simple sequence composition, low immunogenicity, and flexible modification possibilities, in vitro synthesized mRNA has demonstrated strong potential as a candidate for gene therapy. Many efforts have been made to enhance its therapeutic efficacy and safety. Profiting from the development in pathogenesis and materials science, much progress has been achieved in mRNA-based therapy studies. Many mRNA-derived therapeutics including vaccines, antibodies, cytokines, and growth factors have emerged for the treatment of diverse diseases that have multiple modes of action. Novel delivery vectors with enhanced capacity, safety, and properties have been developed to meet the demands of mRNA delivery. Advanced strategies like library screening, environment interaction, and bio-inspiration materials have been used in the investigation process and produced valuable results. In this review, we summarize and discuss recent advances in mRNA-based gene therapy studies.
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Blum and Moradpour. "Recombinant drugs and gene therapy." Therapeutische Umschau 56, no. 12 (December 1, 1999): 730–37. http://dx.doi.org/10.1024/0040-5930.56.12.730.

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Ausgehend von der Biologie somatischer Zellen mit Transkription der chromosomalen DNA in messenger RNA (mRNA) und deren Translation in Proteine werden die Prinzipien der Gentechnologie illustriert. Die Gentechnologie ermöglicht die Klonierung, Identifizierung und Charakterisierung von Genen sowie deren Expression mit Synthese von medizinisch relevanten Proteinen. Die Produkte der Gentechnologie sind somit zum einen klonierte Gene, die für die molekulare Diagnostik und die Gentherapie medizinisch bedeutsam sind. Zum anderen sind es in vitro oder in vivo hergestellte Proteine, die als Diagnostika, Therapeutika und Impfstoffe zunehmend Eingang in die klinische Praxis finden.
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Beltran, Himisha, Alexander Wyatt, Edmund Chedgy, Ladan Fazli, Andrea Sboner, Susan Halabi, and Martin Gleave. "Impact of therapy on gene expression in high-risk prostate cancer (PCA) treated with neoadjuvant docetaxel and androgen deprivation therapy." Journal of Clinical Oncology 34, no. 2_suppl (January 10, 2016): 8. http://dx.doi.org/10.1200/jco.2016.34.2_suppl.8.

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8 Background: Molecular analyses of neoadjuvant post-treatment radical prostatectomy (RP) specimens has been challenging as often times only microscopic foci remain present at time of RP precluding RNA-seq. DNA analysis alone in the absence of expression may be suboptimal in elucidating complex mechanisms of resistance and/or prognostic risk stratification. We therefore set out to develop an assay that could quantify mRNA expression in treated and untreated PCA using formalin fixed paraffin embedded (FFPE) tissues. Methods: We evaluated 40 untreated and post-treatment FFPE specimens as well as patient-matched pre-treated needle biopsies and baseline clinical data from patients enrolled on CALGB 90203: a randomized phase 3 trial comparing noeadjuvant docetaxel and ADT followed by RP vs RP alone for men with high risk localized PCA. High-density tumor areas were selected for RNA extraction (min 50ng RNA). We used NanoString nCounter to quantify gene expression of a custom panel of 75 genes including AR and androgen regulated, neural/neuroendocrine (NE), EMT, cell cycle, hormone receptors, TMPRSS-ERG, ARv7 splice variant, and housekeeper genes. mRNA data was integrated with matched whole exome sequencing data. Frozen specimens and RNA-Seq (n = 7) were used for QC and comparative analysis. Results: Quantitative expression using Nanostring showed high correlation with RNA-seq of patient-matched frozen tissue (Spearman coefficient 0.9). There was significant upregulation of AR and the ARv7 expression following treatment, as well as a subset of NE and EMT genes; three high chromogranin A outlier cases were identified in the treatment arm. There was an overall higher AR score in treated cases (based on expression of 30 AR signaling genes) compared to untreated, along the spectrum of CRPC. Conclusions: These data support the feasibility of quantifying gene expression in neoadjuvant-treated PCA cases with limited FFPE tissue requirement. Extensive characterization of AR status and NE/EMT genes identifies molecular outliers that can arise post-treatment and provides new insight into the heterogeneity of treatment response and potential early markers of resistance. Clinical trial information: NCT00430183.
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DelaFlor-Weiss, E., C. Richardson, M. Ward, A. Himelstein, L. Smith, S. Podda, M. Gottesman, I. Pastan, and A. Bank. "Transfer and expression of the human multidrug resistance gene in mouse erythroleukemia cells." Blood 80, no. 12 (December 15, 1992): 3106–11. http://dx.doi.org/10.1182/blood.v80.12.3106.3106.

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Abstract Gene therapy in humans requires the transplantation of genetically modified cells, and it is important to select only those cells capable of expressing high levels of protein from the transferred gene. Expression of the human multiple drug resistance (MDR) gene confers resistance to a variety of compounds in vitro and in vivo. To determine the feasibility of conferring recipient erythroid cells with the MDR phenotype, we have transduced mouse erythroleukemia cells (MELC) with the MDR gene in a retroviral vector. We show here that MELC clones resistant to exposure to colchicine (an MDR-responsive agent) can be isolated, and demonstrate high levels of MDR RNA and protein expression. Increasing doses of colchicine increase the level of MDR RNA and protein expression significantly. These results indicate that it is possible to transfer and express the human MDR phenotype in mouse erythroid cells by retrovirally mediated gene transfer, and that drug selection can be used to enrich or purify populations of cells containing and expressing this gene.
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DelaFlor-Weiss, E., C. Richardson, M. Ward, A. Himelstein, L. Smith, S. Podda, M. Gottesman, I. Pastan, and A. Bank. "Transfer and expression of the human multidrug resistance gene in mouse erythroleukemia cells." Blood 80, no. 12 (December 15, 1992): 3106–11. http://dx.doi.org/10.1182/blood.v80.12.3106.bloodjournal80123106.

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Gene therapy in humans requires the transplantation of genetically modified cells, and it is important to select only those cells capable of expressing high levels of protein from the transferred gene. Expression of the human multiple drug resistance (MDR) gene confers resistance to a variety of compounds in vitro and in vivo. To determine the feasibility of conferring recipient erythroid cells with the MDR phenotype, we have transduced mouse erythroleukemia cells (MELC) with the MDR gene in a retroviral vector. We show here that MELC clones resistant to exposure to colchicine (an MDR-responsive agent) can be isolated, and demonstrate high levels of MDR RNA and protein expression. Increasing doses of colchicine increase the level of MDR RNA and protein expression significantly. These results indicate that it is possible to transfer and express the human MDR phenotype in mouse erythroid cells by retrovirally mediated gene transfer, and that drug selection can be used to enrich or purify populations of cells containing and expressing this gene.
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Nabilsi, Nancy H., Russell R. Broaddus, Adrienne S. McCampbell, Karen H. Lu, Henry T. Lynch, Lee-may Chen, and David S. Loose. "Sex hormone regulation of survivin gene expression." Journal of Endocrinology 207, no. 2 (August 26, 2010): 237–43. http://dx.doi.org/10.1677/joe-10-0128.

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Survivin (BIRC5) is a cell survival gene that is overexpressed in endometrial cancer and has been implicated to have a physiological role in normal endometrial function. To determine whether survivin gene expression is regulated by reproductive steroid hormones in the human endometrium, RNA was prepared from normal cycling women in the proliferative and secretory phases of the menstrual cycle. RNA was also isolated from 21 endometrial biopsies from premenopausal women at baseline and following 3 months of treatment with depot medroxyprogesterone acetate. Finally, RNA was isolated from endometrial biopsies from ten healthy postmenopausal women participating in a clinical trial of estrogen replacement therapy at baseline and following 6 months of treatment with conjugated equine estrogen. Quantitative RT-PCR analysis was used to determine survivin, insulin-like growth factor binding protein 1 (IGFBP1), Ki67, and IGF1 gene expression levels. Survivin gene expression was highest in the proliferative phase of the menstrual cycle and showed a statistically significant 4-fold increase in expression following chronic treatment with estrogens; this was strongly correlated with increased Ki67, a marker of proliferation. Survivin gene expression decreased 4.6-fold following chronic progestin treatment in the human endometrium. These data suggest that survivin transcript is regulated by estrogens and progestins in the disease-free human endometrium. The data also suggest that survivin transcript may be used as a biomarker of estrogen and progestin treatment efficacy, but validation studies must be conducted to support this conclusion.
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Dissertations / Theses on the topic "RNA; Gene expression; Therapy"

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Maniotis, Despina. "Investigation of hammerhead ribozyme function and potential in the central nervous system." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365768.

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Baughan, Travis Lorson Christian. "Gene therapy in spinal muscular atrophy RNA-based strategies to modulate the pre-mRNA splicing of survival motor neuron /." Diss., Columbia, Mo. : University of Missouri-Columbia, 2008. http://hdl.handle.net/10355/6686.

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The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from PDF of title page (University of Missouri--Columbia, viewed on March 10, 2010). Vita. Thesis advisor: Lorson, Christian L. "December 2008" Includes bibliographical references
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Ramachandran, Shyam. "Regulation Of gene expression in cystic fibrosis: implications for biology and therapeutics." Diss., University of Iowa, 2012. https://ir.uiowa.edu/etd/2613.

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Cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel that when mutated causes the disease cystic fibrosis (CF). Many obstacles hinder the understanding of CF disease pathogenesis, impeding advancements in understanding how mutations cause disease, and slowing the progress towards new treatments. To this end, we have profiled the transcriptome (mRNA and microRNA) of human and newborn pig CF and non-CF airway epithelia. We show that the use of cross-species transcriptomics allows the identification of genes differentially expressed owing to the loss of CFTR, and not due to confounding environmental or secondary disease progression influences. The identification of reduced OAS1 expression in CF samples is a case in point. We also demonstrate the utility of transcriptome profiling and longitudinal studies in pigs, providing greater understanding of the molecular mechanisms underlying CF disease progression. MicroRNAs (miRNAs) comprise a large family of ~21-nt long non-coding RNAs that function as key post-transcriptional regulators of gene expression. Very little is known of how CFTR is regulated in the cell, both transcriptionally and post-transcriptionally. We discovered three miRNAs: miR-509-3p, miR-494 and miR-138 with possible CFTR regulatory functions. miR-509-3p or -494 directly target the CFTR mRNA, and decrease CFTR levels when over expressed; while inhibiting them had the opposite effect. Upon stimulating human airway epithelial cells with TNFα or IL-1β, we observed an increase in expression of both miRNAs mediated in part by the NF-κB transcription factor complex, with a concurrent decrease in CFTR expression. Gene ontology classification of predicted targets of miR-509-3p and/or miR-494 expressed in the airway epithelium revealed enrichment for genes in ion transport pathways. To our knowledge, this is the first suggestion of a possible role for miRNAs regulating a broad range of important epithelial electrolyte and fluid transport proteins. The study of miR-138 mediated regulation of CFTR expression has led to novel discoveries in the field of CFTR transcriptional control. We discovered SIN3A to be a novel transcriptional repressor of CFTR, interacting with CTCF on the CFTR promoter at the -20.9 kb DHS. By validating SIN3A as a conserved target of miR-138, we also discovered miR-138 to be a novel transcriptional regulator/activator of CFTR. The most common CFTR mutation, ΔF508, causes protein misfolding, degradation, and CF. Manipulating the miR-138/SIN3A regulatory network improved the biosynthesis of CFTR-ΔF508, restoring Cl- transport to human CF airway epithelia. To our knowledge, this is the first example of an individual miRNA having such broad regulatory functions. This discovery also provided novel targets for restoring CFTR function in cells affected by the most common CF mutation. To this end, we are utilizing the molecular signatures of miR-138 over-expression and SIN3A knockdown to identify candidate genes for RNA interference screens, and to identify candidate small molecule drugs that might mimic the effects of these two interventions. The goal of this approach is to develop a new therapeutic agent that restores anion transport to airway epithelia and other cell types and tissues affected by CF.
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PEREIRA, LARISSA M. "Clonagem, expressao, purificacao e caracterizacao estrutural da proteina ribossomal L10 humana recombinante." reponame:Repositório Institucional do IPEN, 2009. http://repositorio.ipen.br:8080/xmlui/handle/123456789/9478.

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Dissertacao (Mestrado)
IPEN/D
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
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Issa, Mohamed Mahmoud. "Linear and Branched Chitosan Oligomers as Delivery Systems for pDNA and siRNA In Vitro and In Vivo." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7376.

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Hamilton, Andrew John. "Inhibiting gene expression with anti-sense RNA." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316938.

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Sesto, Nina. "RNA mediated gene expression regulation in Listeria." Paris 7, 2012. http://www.theses.fr/2012PA077233.

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Listeria monocytogenes est une bactérie pathogène responsable de la listériose, une maladie d'origine alimentaire. Alors que de nombreuses protéines de L. Monocytogenes ont été identifiées comme facteurs de virulence, le rôle des petits ARN régulateurs chez Listeria demeure méconnu. Mon projet de thèse avait pour objectif de découvrir de nouveaux concepts sur la régulation de l'expression des gènes impliquant des ARNs régulateurs jouant un rôle dans la virulence de Listeria. Mon travail a été basé sur deux approches distinctes. La première a concerné l'analyse comparative des transcriptomes de L. Monocytogenes et de L. Innocua, une espèce proche non-pathogène. La seconde a concerné la caractérisation de petits ARNs régulateurs et l'identification de leur rôle dans le processus infectieux. L'analyse comparative des deux transcriptomes a révélé une conservation de la majorité des transcrits, à l'exception d'une divergence significative entre les deux espèces pour un sous-ensemble d'ARN régulateurs non-codants. De manière surprenante, nous avons identifié une classe de longs transcrits antisens (lasARNs) qui chevauchent un gène tout en servant de S'UTR au gène adjacent divergent. La transcription du lasARN est à l'origine d'une régulation mutuellement exclusive de l'expression des gènes adjacents avec des fonctions opposées. Cette étude a été la première à comparer deux transcriptomes bactériens avec une résolution d'une seule base. Elle a conduit à la découverte chez L. Monocytogenes de 33 nouveaux petits ARNs et 53 asARNs et à l'identification d'une structure lasRNA /opéron que nous avons appelé « excludon» qui pourrait représenter une nouvelle forme de régulation chez les bactéries. J'ai également effectué une analyse sur plusieurs petits ARNs en combinant des approches de transcriptomes, des modèles d'infection chez la souris et en utilisant des algorithmes de prédiction de cibles des ARNs. J'ai ainsi identifié et caractérisé cinq petits ARNs qui sont importants pour la virulence de Listeria. Au cours de cette analyse, j'ai caractérisé en détail RliB, un ARN à double fonction pouvant agir comme un élément CRISPR et comme un ARN régulateur impliqué dans la régulation de l'homéostasie du fer chez Listeria. Nous avons ainsi réalisé la première étude détaillée d'un élément CRISPR impliqué dans la régulation d'un processus cellulaire fondamental autres que l'immunité contre les bactériophages
Listeria monocytogenes is a bacterial pathogen responsible for listeriosis, a food-borne disease. In contrast to the significant advances in identifying proteins involved in virulence, relatively little is known about the role of sRNAs in L. Monocytogenes pathogenesis. The aim of my thesis project was to discover new concepts in RNA-mediated gene expression regulation with a role in Listeria virulence. My work was based on two distinct approaches. The first one involved global transcriptomic studies of L. Monocytogenes and its non-pathogenic relative, Listeria innocua while the second one concerned the characterization of individual sRNAs and the identification of their role in the infectious process. First, our comparative transcriptome analysis revealed conservation across most transcripts, but significant divergence between the species in a subset of non-coding sRNAs. This study was the first to compare two bacterial transcriptomes at a single-base resolution. Pt led to the discovery of 33 new sRNAs and 53 new asRNAs in L. Monocytogenes. Remarkably, we identified a class of long antisense transcripts (lasRNAs) that overlap one gene while also serving as the 5'UTR of the adjacent divergent gene. IasRNA transcription leads to the mutually exclusive regulation of the adjacent genes with opposite functions. This IasRNA/operon structure that we named "excludon" might represent a novel form of regulation in bacteria. Second, we conducted an analysis on several sRNAs by combining transcriptomic approaches, target prediction algorithm and mouse model of infection. I thus identified and characterized five sRNAs that are important for Listeria virulence. Furthermore, I focused on the detailed characterization of RIiB, a dual-function regulatory RNA that acts as a CRISPR element and a regulatory RNA involved in Listeria iron homeostasis regulation, providing the first detailed study of CRISPR element regulating fundamental cellular processes other than acting as a bacteriophage defense system
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Nwokeoha, Sandra. "Lithotripter shock wave induced RNA-based gene therapy." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:89b67c7d-4b1c-4254-8e10-86a8e6c6c79d.

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Gene therapy is the process of introducing genes to augment or minimise the expression of absent or defective genes, respectively. Non-viral gene delivery systems for the treatment of genetic diseases, have evolved into highly appreciable nucleic acid-based therapies due to considerably less risk of host immunogenicity and induction of inflammatory responses. However, they are challenged by limited delivery. Thus, more efficient strategies are continually being sought. Lithotripter shock waves (LSW) are powerful acoustic waves that are an attractive choice of delivery system, as they offer a non-invasive, targeted and safe approach. Furthermore, the delivery of messenger ribonucleic acid (mRNA) possesses several advantages over commonly delivered plasmid deoxyribonucleic acids (pDNA), because it does not require opening of the nuclear envelope, thereby reducing the level of cell injury necessary for transfection. This work presents the first investigation on the efficacy of LSW mediated mRNA delivery, based on optimised SW parameters that balance the desired enhanced permeability of cell membranes against undesired cytotoxicity, and maintain the structural and biological stability of the RNA. A transfectability measure that defines the ability of SWs to permeabilise a cell whilst keeping it alive was established for dissimilar cell types, as a function of the acoustic pressure and number of SWs. Statistically significant RNA uptake was recorded in a tissue mimicking system, and using RNA analogues at various concentrations, the SW induced bio-distribution was characterised. In addition to LSW induced gene augmentation using mRNA, it was shown that LSWs could be used to effect gene inhibition through the delivery of siRNA. Kinetic experiments were carried out to measure mRNA uptake during shock wave exposure and indicated that rate of delivery was highest at the start of the SW dose and decreased during treatment. The results also suggested that the enhancement of cell permeability was significantly transient, and that mRNA was highly susceptible to degradation in its naked state. Furthermore, mRNA-based gene expression was shown to be predictive but quantal. The in vitro tissue model was improved from a gel-based system, to one that incorporated multi-cellular spheroids which capture aspects of 3-D tumours. Static overpressure was applied during SW exposure in order to suppress cavitation effects and isolate effects that could be attributed to shear due to cell-to-cell coupling. The results showed that mild overpressure improved RNA uptake the most, but that at higher overpressure, the level of increase in RNA uptake relative to controls, was dependent on the type of RNA nucleotide being delivered. This suggested that a complex interaction between LSW cavitation and direct stress dominates delivery. A final report was on the significant improvement of gene delivery when mRNA was encapsulated within a lipid nanoparticle vector, and SW exposure was assisted by cavitation agents. Also, by exposure to another acoustic stimulus - focused ultrasound (US), direct comparisons were made between SWs and US on the efficiency of delivery and tissue penetration. In conclusion, this thesis has shown that by choosing parameters appropriately, shock waves can be a promising strategy for the delivery of genes to cells.
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Rück, Andreas. "Myocardial gene therapy and gene expression in angina pectoris /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-648-4/.

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Preuten, Tobias. "Organellar gene expression." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2010. http://dx.doi.org/10.18452/16142.

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Zusätzlich zu der eubakteriellen RNA-Polymerase (RNAP) der Plastiden sind im Zellkern von Arabidopsis thaliana drei weitere, phagentypische RNAP kodiert, die jeweils aus nur einer Einheit aufgebaut sind. Die Enzyme RpoTp und RpoTm werden in die Plastiden, bzw. die Mitochondrien transportiert, während RpoTmp in beiden Organellen zu finden ist. Um die Lichtabhängigkeit der RpoT-Gene zu untersuchen, wurde die lichtinduzierte Akkumulation ihrer Transkripte in 7-Tage alten Keimlingen, sowie 3- bzw. 9-Wochen alten Rosettenblättern mittels quantitativer real-time PCR ermittelt. Die entwicklungsabhängige Regulation der RpoT-Transkript-Akkumulation wurde außerdem während der Blattentwicklung analysiert. Zusätzlich wurde der Einfluss des circadianen Rhythmus untersucht. Es stellte sich heraus, dass die Transkriptakkumulation aller drei RpoT-Gene stark lichtinduziert war und nur marginalen circadianen Schwankungen unterlag. In weiteren Versuchen mit verschiedenen Lichtrezeptor-Mutanten und unterschiedlichen Lichtqualitäten wurde der Einfluss multipler Rezeptoren auf den Prozess der Lichtinduktion gezeigt. In den Zellen höherer Pflanzen finden sich drei Genome. Die Biogenese von Chloroplasten und Mitochondrien, sowie lebenswichtige Prozesse, wie Atmung und Photosynthese setzen oftmals die Aktivität von Genen auf mindestens zwei dieser Genome voraus. Eine intrazelluläre Kommunikation zwischen den verschiedenen Genomen ist daher unumgänglich für einen funktionierenden Stoffwechsel der Pflanze. In dieser Arbeit wurde herausgestellt, dass die Zahl mitochondrialer Genkopien in photosynthetisch inaktiven Arabidopsis-Keimlingen drastisch erhöht ist. Bei der Untersuchung des DNA-Gehaltes in Proben, die Altersstufen von 2-Tage alten Keimblättern bis hin zu 37-Tage alten, seneszenten Rosettenblättern umfassten, fand sich ein deutlicher Anstieg der Kopienzahlen in älteren Rosettenblättern. Außerdem unterschieden sich die Kopienzahlen der untersuchten Gene zum Teil erheblich voneinander.
In addition to eubacterial-like multi-subunit RNA polymerases (RNAP) localized in plastids and the nucleus, Arabidopsis thaliana contains three phage-like single-unit, nuclear-encoded, organellar RNAPs. The enzymes RpoTp and RpoTm are imported into plastids and mitochondria, respectively, whereas RpoTmp shows dual targeting properties into both organelles. To investigate if expression of the RpoT genes is light-dependent, light-induced transcript accumulation of RpoTm, RpoTp and RpoTmp was analyzed using quantitative real-time-PCR in 7-day-old seedlings as well as in 3- and 9-week-old rosette leaves. To address the question whether RpoT transcript accumulation is regulated differentially during plant development transcript abundance was measured during leaf development. Additionally, effects of the plants circadian rhythm on RpoT transcript accumulation were analyzed. Transcripts of all three RpoT genes were found to be strongly light-induced even in senescent leaves and only marginally influenced by the circadian clock. Further analyses employing different photoreceptor mutants and light qualities revealed the involvement of multiple receptors in the light-induction process. The biogenesis of mitochondria and chloroplasts as well as processes like respiration and photosynthesis require the activity of genes residing in at least two distinct genomes. There have to be ways of intracellular communication between different genomes to control gene activities in response to developmental and metabolic needs of the plant. In this study, it was shown that gene copy numbers drastically increased in photosynthetically inactive Arabidopsis seedlings. Mitochondrial DNA contents in cotyledons and leaves ranging in age from 2-day-old cotyledons to 37-day-old senescent rosette leaves were examined. A common increase in senescing rosette leaves and drastic differences between individual genes were found, revealing the importance of an integrative chondriome in higher plant cells.
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Books on the topic "RNA; Gene expression; Therapy"

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service), SpringerLink (Online, ed. MicroRNA Interference Technologies. Berlin, Heidelberg: Springer-Verlag Berlin Heidelberg, 2009.

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Yves, Christen, and SpringerLink (Online service), eds. Macro Roles for MicroRNAs in the Life and Death of Neurons. Berlin, Heidelberg: Springer-Verlag Berlin Heidelberg, 2010.

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Slack, Frank J. MicroRNAs in development and cancer. London: Imperial College Press, 2011.

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Clouet-d'Orval, Béatrice, ed. RNA Metabolism and Gene Expression in Archaea. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-65795-0.

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Koloteva, Nadejda. Regulation of eukaryotic gene expression via RNA-RNA and RNA-protein interactions. Manchester: UMIST, 1997.

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Wang, Zhiguo. MicroRNA expression detection methods. Heidelberg: Springer, 2010.

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J, Kay, Ballard F. J, and Mayer R. J, eds. Gene expression: Regulation at the RNA and protein levels. London: Biochemical Society, 1989.

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F, Gesteland Raymond, and SpringerLink (Online service), eds. Recoding: Expansion of Decoding Rules Enriches Gene Expression. New York, NY: Springer Science+Business Media, LLC, 2010.

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NATO/CEC, Advanced Research Workshop on "Post-Transcriptional Control of Gene Expression" (1990 Goslar Germany). Post-transcriptional control of gene expression. Berlin: Springer-Verlag, 1990.

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Next-generation MicroRNA expression profiling technology: Methods and protocols. New York: Humana Press, 2012.

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Book chapters on the topic "RNA; Gene expression; Therapy"

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Maeda, Yukihide, Abraham M. Sheffield, and Richard J. H. Smith. "Therapeutic Regulation of Gene Expression in the Inner Ear using RNA Interference." In Gene Therapy of Cochlear Deafness, 13–36. Basel: KARGER, 2009. http://dx.doi.org/10.1159/000218205.

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Björklund, Tomas. "Expression of Multiple Functional RNAs or Proteins from One Viral Vector." In Gene Therapy for Neurological Disorders, 41–56. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3271-9_3.

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Chubb, Jonathan R., Michelle Stevense, Danielle Cannon, Tetsuya Muramoto, and Adam M. Corrigan. "Imaging Nascent RNA Dynamics in Dictyostelium." In Imaging Gene Expression, 101–13. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-526-2_8.

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Nordström, Kurt, Stanley N. Cohen, and Robert W. Simons. "Antisense RNA." In Post-transcriptional Control of Gene Expression, 231–61. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-60929-9_20.

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Tomizawa, Minoru, Julie Lekstrom-Himes, and Kleanthis G. Xanthopoulos. "Transcriptional Regulation and Gene Expression in the Liver." In Gene Therapy, 17–36. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-72160-1_2.

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Glass, R. E., and V. Nene. "Genetic Dissection of E.coli RNA Polymerase." In Gene Manipulation and Expression, 155–72. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-011-6565-5_12.

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Anderegg, B., A. Irie, and K. J. Scanlon. "Ribozymes as Biotherapeutic Tools for the Modulation of Gene Expression." In Gene Therapy, 97–130. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-662-03577-1_6.

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Spížek, J., P. Ryšavý, M. Klégr, J. Náprstek, J. Janećek, and P. Tichý. "DNA-Dependent RNA Polymerase from Streptomyces Granaticolor." In Gene Manipulation and Expression, 196–208. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-011-6565-5_15.

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Buff, Maximilian C. R., Stefan Bernhardt, Musa D. Marimani, Abdullah Ely, Joachim W. Engels, and Patrick Arbuthnot. "Use of Guanidinopropyl-Modified siRNAs to Silence Gene Expression." In RNA Interference, 217–49. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1538-5_13.

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Lai, Yi, and Dongsheng Duan. "Design of Muscle Gene Therapy Expression Cassette." In Muscle Gene Therapy, 141–56. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-03095-7_8.

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Conference papers on the topic "RNA; Gene expression; Therapy"

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Varadan, V., S. Kamalakaran, A. Janevski, N. Banerjee, K. Lezon-Geyda, V. Bossuyt, D. Flowers, et al. "P3-06-01: Next Generation RNA Sequencing Reveals Changes in Gene Expression and Alternative Splicing upon Brief Exposure to Therapy in Early Breast Cancer." In Abstracts: Thirty-Fourth Annual CTRC‐AACR San Antonio Breast Cancer Symposium‐‐ Dec 6‐10, 2011; San Antonio, TX. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/0008-5472.sabcs11-p3-06-01.

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Lau, Rosanna, Lily Fu, Michael Samuels, Rashmi K. Murthy, Bruno Sinn, Jane Yu, Rebekah Gould, et al. "Abstract 1796: A targeted RNA-seq assay to measure activating ER mutations and ER/PR-associated gene expression predicts sensitivity to endocrine therapy for metastatic breast cancer." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-1796.

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Ayers, Mark D., Michael Nebozhyn, Heather A. Hirsch, Razvan Cristescu, Erin E. Murphy, S. Peter Kang, Scot W. Ebbinghaus, Terrill K. McClanahan, Andrey Loboda, and Jared K. Lunceford. "Abstract 1307: Assessment of gene expression in peripheral blood from patients with advanced melanoma using RNA-seq before and after treatment with anti-PD-1 therapy with pembrolizumab (MK-3475)." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-1307.

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Kornev, A. A., V. V. Vysochinskaya, N. A. Knyazev, A. K. Emel'yanov, and A. A. Bogdanov. "Transfection of human peripheral blood T-lymphocytes with synthetic small interfering RNAs: selection of an effective technique." In Global science. Development and novelty. L-Journal, 2020. http://dx.doi.org/10.18411/gsdn-25-12-2020-04.

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Abstract:
It has been shown that the state of the human immune system and its activity determines the development, course and prognosis of many oncological diseases. To date, various immunotherapeutic approaches have been developed, of which the most promising is adoptive cell therapy (ACT). An increase in the effectiveness of this type of therapy could be achieved by using synthetic small interfering RNAs (siRNAs) to suppress the expression of key immune checkpoint (ICI) genes (PD1, CTL4) in T-lymphocytes, which are involved in cellular immunosuppression. However, there is a problem of selecting an effective method for delivering such siRNAs to T-lymphocytes. In the present study, we developed a synthetic siRNA specific to the mRNA sequence of the PD1 gene and evaluated the efficiency of its delivery to activated human T-lymphocytes using chemically modified siRNA, histone H1, – 18 – Global science. Development and novelty and the liposomal agent HiperFect. Ex vivo activated T-lymphocytes from healthy donors were used. The efficiency of siRNA transfection into cells and its confirmation was assessed using flow cytofluorometry and confocal microscopy. As a result of the study, a high (51%) efficiency of transfection into these cells using chemically modified "self-delivering" siRNA was shown. For other methods of delivery of miRNA to T-lymphocytes, low efficiency is shown. Thus, our data suggest that of the three approaches used in this work for miRNAs delivery to activated T-lymphocytes, the most effective is the use of “self-delivering” chemically modified cholesterol miRNA.
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Harati, Sahar, John H. Phan, and May D. Wang. "Investigation of factors affecting RNA-seq gene expression calls." In 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2014. http://dx.doi.org/10.1109/embc.2014.6944805.

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Wang, Tianyu, and Sheida Nabavi. "Differential gene expression analysis in single-cell RNA sequencing data." In 2017 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2017. http://dx.doi.org/10.1109/bibm.2017.8217650.

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He, Mu-yang, Hao-jie Cao, Le Wang, and Shou-jing Zhao. "Construction of Ginseng CS Gene RNA Interference Plant Expression Vector." In 2012 International Conference on Biomedical Engineering and Biotechnology (iCBEB). IEEE, 2012. http://dx.doi.org/10.1109/icbeb.2012.115.

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Po-Yen Wu, J. H. Phan, Fengfeng Zhou, and M. D. Wang. "Evaluation of normalization methods for RNA-Seq gene expression estimation." In 2011 IEEE International Conference on Bioinformatics and Biomedicine Workshops (BIBMW). IEEE, 2011. http://dx.doi.org/10.1109/bibmw.2011.6112354.

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Ei-Wen Yang, Thomas Girkes, and Tao Jaing. "Differential gene expression analysis using coexpression and RNA-Seq data." In 2013 IEEE 3rd International Conference on Computational Advances in Bio and Medical Sciences (ICCABS). IEEE, 2013. http://dx.doi.org/10.1109/iccabs.2013.6629222.

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Yang, Cheng, Po-Yen Wu, Li Tong, John Phan, and May Wang. "The impact of RNA-seq aligners on gene expression estimation." In BCB '15: ACM International Conference on Bioinformatics, Computational Biology and Biomedicine. New York, NY, USA: ACM, 2015. http://dx.doi.org/10.1145/2808719.2808767.

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Reports on the topic "RNA; Gene expression; Therapy"

1

Ljungman, Mats. Use of Nascent RNA Microarrays to Study Inducible Gene Expression in Breast Cancer Cells. Fort Belvoir, VA: Defense Technical Information Center, September 2005. http://dx.doi.org/10.21236/ada443027.

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Vang, Lindsay K., P. Scott Pine, Sarah A. Munro, and Marc L. Salit. Preparation of a set of total RNA benchmarking samples for performance assessment of genome-scale differential gene expression. Gaithersburg, MD: National Institute of Standards and Technology, June 2017. http://dx.doi.org/10.6028/nist.sp.1200-23.

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Curiel, David T., Gene Siegal, and Minghui Wang. A Double Selection Approach to Achieve Specific Expression of Toxin Genes for Ovarian Cancer Gene Therapy. Fort Belvoir, VA: Defense Technical Information Center, November 2007. http://dx.doi.org/10.21236/ada485589.

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Curiel, David T., Gene Siegal, and Minghui Wang. A Double Selection Approach to Achieve Specific Expression of Toxin Genes for Ovarian Cancer Gene Therapy. Fort Belvoir, VA: Defense Technical Information Center, November 2006. http://dx.doi.org/10.21236/ada472761.

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Reddy, Sakamuri V. Measles Virus Nucleocapsid (MVNP) Gene Expression and RANK Receptor Signaling in Osteoclast Precursors, Osteoclast Inhibitors Peptide Therapy for Pagets Disease. Fort Belvoir, VA: Defense Technical Information Center, October 2008. http://dx.doi.org/10.21236/ada500887.

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Reddy, Sakamuri V. Measles Virus Nucleocapsid (MJVNP) Gene Expression and RANK Receptor Signaling in Osteoclast Precursors. Osteoclast Inhibitors Peptide Therapy for Pagets Disease. Fort Belvoir, VA: Defense Technical Information Center, October 2005. http://dx.doi.org/10.21236/ada484715.

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Gomer, Charles J. Photodynamic Therapy Oxidative Stress as a Molecular Switch Controlling Therapeutic Gene Expression for the Treatment of Locally Recurrent Breast Carcinoma. Fort Belvoir, VA: Defense Technical Information Center, June 2001. http://dx.doi.org/10.21236/ada396793.

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Reddy, Sakamuri V. Measles Virus Nucleocapsid (MVNP) Gene Expression and RANK Receptor Signaling in Osteoclast Precursors, Osteoclast Inhibitors Peptide Therapy for Pagets Disease. Fort Belvoir, VA: Defense Technical Information Center, October 2006. http://dx.doi.org/10.21236/ada462833.

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Reddy, Sakamuri V. Measles Virus Nucleocapsid (MVNP) Gene Expression and RANK Receptor Signaling in Osteoclast Precursors,Osteoclast Inhibitors Peptide Therapy for Pagets Disease. Fort Belvoir, VA: Defense Technical Information Center, October 2004. http://dx.doi.org/10.21236/ada482539.

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