Academic literature on the topic 'RNA fingerprinting'

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Journal articles on the topic "RNA fingerprinting"

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Gegenheimer, Peter. "Electronic fingerprinting of RNA." Nucleic Acids Research 16, no. 5 (1988): 1799–800. http://dx.doi.org/10.1093/nar/16.5.1799.

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Gegenheimer, Peter. "Electronic fingerprinting of RNA." Nucleic Acids Research 16, no. 5 (1988): 1801–12. http://dx.doi.org/10.1093/nar/16.5.1801.

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Kato, K. "RNA fingerprinting by molecular indexing." Nucleic Acids Research 24, no. 2 (January 15, 1996): 394–95. http://dx.doi.org/10.1093/nar/24.2.394.

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Singh, Ankita, Akhilesh Mishra, Ali Khosravi, Garima Khandelwal, and B. Jayaram. "Physico-chemical fingerprinting of RNA genes." Nucleic Acids Research 45, no. 7 (December 8, 2016): e47-e47. http://dx.doi.org/10.1093/nar/gkw1236.

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Welsh, John, Kiran Chada, Seema S. Dalal, Rita Cheng, David Relph, and Michael McClelland. "Arbitrarily primed PCR fingerprinting of RNA." Nucleic Acids Research 20, no. 19 (1992): 4965–70. http://dx.doi.org/10.1093/nar/20.19.4965.

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McClelland, M., and J. Welsh. "RNA fingerprinting by arbitrarily primed PCR." Genome Research 4, no. 1 (August 1, 1994): S66—S81. http://dx.doi.org/10.1101/gr.4.1.s66.

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Carter, R. E., J. H. Wetton, and D. T. Parkin. "Improved gentic fingerprinting using RNA probes." Nucleic Acids Research 17, no. 14 (1989): 5867. http://dx.doi.org/10.1093/nar/17.14.5867.

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Rymerson, R. T., R. P. Bodnaryk, S. Haber, and J. D. Procunier. "Arbitrary primed RNA fingerprinting in plants." Biotechnology Techniques 9, no. 8 (August 1995): 563–66. http://dx.doi.org/10.1007/bf00152444.

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Augéde Mello, P., R. C. Olascoaga, M. P. Costa Giomi, A. Alonso Fernández, E. A. Scodeller, J. L. La Torre, and I. E. Bergmann. "RNA fingerprinting of South American prototype aphthovirus strains." Vaccine 4, no. 2 (June 1986): 105–10. http://dx.doi.org/10.1016/0264-410x(86)90047-2.

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Lozano, Gloria, Reyes Jimenez-Aparicio, Santiago Herrero, and Encarnacion Martinez-Salas. "Fingerprinting the junctions of RNA structure by an open-paddlewheel diruthenium compound." RNA 22, no. 3 (January 12, 2016): 330–38. http://dx.doi.org/10.1261/rna.054353.115.

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Dissertations / Theses on the topic "RNA fingerprinting"

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Hurtado, Ana Isabel. "Large-subunit ribosomal RNA gene of Helicobacter and Campylobacter species : its role in genotypic identification and typing." Thesis, Queen Mary, University of London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265831.

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PROCACCIANTI, CLAUDIO. "Quantitative evaluation of in vitro transformation by analysis of morphological and biochemical markers and statistical descriptors." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2012. http://hdl.handle.net/10281/28450.

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The EC directive REACH (EC1907/2006) sets, amongst others, the need for all the chemicals to be tested for their carcinogenic potential. In vitro assays can provide a fast and reliable tool for screening purposes. The Cell Transformation Assay (CTA) is one of the in vitro assays in the most advanced phase of the validation process and the only one able to evaluate both the genotoxic and the non-genotoxic potentials. The evaluation of results of the CTA is based on the scoring of transformed colonies (foci) by a trained expert on the basis of their morphological features. Levels of cell packing and multilayered growth, as well as fibroblastic shape of cells, criss-crossing and invasion of the surrounding monolayer features are evaluated for classification. While the decision making process is based on standard criteria, their interpretation is potentially biased, especially in borderline cases, due to a certain degree of subjectivism inherent in the evaluation of qualitative features. This aspect is critical towards the international validation of the CTA assay: subjectivity driven error might in fact result in under or over estimation of the carcinogenic potential of tested compounds. In this work, different approaches were used to develop an objective method to give decisional support to the operator in the classification procedure. Biological markers related to the transformation process (p53, cx43), and to a general cell stress (Hsp70) were analyzed. A novel technique for the in focus localization of biological markers of transformation was developed. RNA whole genome screening was used to set the conditions for future molecular characterization of foci-derived cell lines. A novel, Quantitative Index of Dissimilarity has been obtained by statistical descriptors capturing morphological features and employing an unsupervised image analysis approach, in order to help the operator in the decisional process of scoring the borderline cases.
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Beatini, Salvatore J. "Using DNA fingerprinting to assess genetic structure of the vernal pool amphibian rana sylvatica." Link to electronic thesis, 2003. http://www.wpi.edu/Pubs/ETD/Available/etd-0428103-153026.

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Thesis (M.S.)--Worcester Polytechnic Institute.
Keywords: wood frog; vernal pool conservation; fragmented habitat; Rana sylvatica; DNA fingerprinting. Includes bibliographical references (p. 38-40).
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Nai, YH. "Capillary electrophoresis of ribosomal RNA for characterisation of microbial communities." Thesis, 2013. https://eprints.utas.edu.au/17552/1/front-_Nai-_thesis.pdf.

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This thesis documents research on new capillary electrophoresis (CE) based rRNA fingerprint approaches for characterisation of low diversity microbial communities. In the first body of work, an alternative approach for sieving polymer synthesis through reversible addition fragmentation chain transfer (RAFT) polymerisation is presented. Sieving polymer matrices are typically synthesised by conventional free radical polymerisation. This thesis describes the first synthesis of a high molecular weight poly(n,n-dimethylacrylamide) (PDMA) in which both the molar mass and polydispersity distribution were controlled by RAFT polymerisation. A multi-step chain extension is detailed and the physical properties and separation performance of DNA/RNA using this RAFT polymer are described. The second body of work deals with the development of new approach for characterisation of microbial communities using CE. The new approach involves conformational separation of microbial 16S ribosomal RNA (rRNA) molecules containing the highly variable regions present in 16S rRNA. Single stranded conformation polymorphism (SSCP) is a separation technique based on the principle that for nucleic acid fragments of equal lengths, variation in sequences can affect nucleic acid folding and hence can be separated due to the difference in electrophoretic mobility. While CE DNA-SSCP has been commonly applied in clinical mutation diagnostic tests and studies of microbial diversity, CE rRNA-SSCP has yet to be demonstrated. In this work, an enzymatic based RNA-oligonucleotide cleavage method was employed to cleave the 16S rRNA (~1542 bases) to smaller fragments of similar length (~340 bases). This strategy uses a eubacterial ‘scissor’ probe to target and hybridise highly conserved sites within the rRNA flanking highly variable regions (e.g. V1, V2 or V3). As rRNA is synthesised only by actively-growing cells, together with its role as the marker molecule for assigning sequences to genera and species, it can thus be used to correlate to the functioning members of microbial communities. Taking advantage of these unique properties, CE-rRNA-SSCP circumvents the need for polymerase chain reaction (PCR) amplification and retains the quantitative information regarding to the evenness of the microbial community that is important for ecological studies that were otherwise lost during PCR step. Compared to gel electrophoresis based approach, CE- rRNA SSCP significantly decreased the analysis time from 24 hours to 60 min and the use of a fluorescently labelled hybridisation probe for detection decreased the sample requirement by ten-fold. The combination of fast analysis time, low sample requirement and sensitive fluorescence detection makes CE-rRNA-SSCP an appealing new approach for characterising low diversity microbial communities. The third body of work deals with the conception and development of a novel characterisation approach termed multiplex cleavage microbial community analysis (MCMCA), which is a potential method to simultaneously link the phylogeny of multiple groups of metabolically active microorganisms to their respective metabolic activity and relative abundance within a community. MCMCA utilizes the similar sequence-specific cleavage of rRNA molecules with oligonucleotides and RNase H employed in previous approach but differs by the use of multiple taxon specific probes selected to specifically cut the 16S rRNA into discrete fragments varying in length. The cleaved rRNA mixture is subsequently mixed with a fluorescently labelled locked nucleic acid (LNA) universal hybridisation probe and resolved using denaturing CE size separation. The feasibility of this rational is tested using model microbial strains, followed by optimisation of the cleavage procedure to achieve multiplex cleavage in a model microbial community. This approach was then applied to characterise a hydrocarbon degrading enrichment community derived from soil.
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Rumpler, Gerhard [Verfasser]. "Molekulare Determinanten des Melanomwachstums, der Invasion und der Metastasierung : eine Analyse basierend auf RNA-Fingerprinting und cDNA-Arrays / vorgelegt von Gerhard Rumpler." 2002. http://d-nb.info/964444968/34.

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Hoang, Minh Tu. "WiFi fingerprinting based indoor localization with autonomous survey and machine learning." Thesis, 2020. http://hdl.handle.net/1828/12091.

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The demand for accurate localization under indoor environments has increased dramatically in recent years. To be cost-effective, most of the localization solutions are based on the WiFi signals, utilizing the pervasive deployment of WiFi infrastructure and availability of the WiFi enabled mobile devices. In this thesis, we develop completed indoor localization solutions based on WiFi fingerprinting and machine learning approaches with two types of WiFi fingerprints including received signal strength indicator (RSSI) and channel state information (CSI). Starting from the low complexity algorithm, we propose a soft range limited K nearest neighbours (SRL-KNN) to address spatial ambiguity and the fluctuation of WiFi signals. SRL-KNN exploits RSSI and scales the fingerprint distance by a range factor related to the physical distance between the user’s previous position and the reference location in the database. Although utilizing the prior locations, SRL-KNN does not require knowledge of the exact moving speed and direction of the user. Besides, the idea of the soft range limiting factor can be applied to all of the existed probabilistic methods, i.e., parametric and nonparametric methods, to improve their performances. A semi-sequential short term memory step is proposed to add to the existed probabilistic methods to reduce their spatial ambiguity of fingerprints and boost significantly their localization accuracy. In the following research phase, instead of locating user's position one at a time as in the cases of conventional algorithms, our recurrent neuron networks (RNNs) solution aims at trajectory positioning and takes into account of the relation among RSSI measurements in a trajectory. The results using different types of RNN including vanilla RNN, long short-term memory (LSTM), gated recurrent unit (GRU) and bidirectional LSTM (BiLSTM) are presented. Next, the problem of localization using only one single router is analysed. CSI information will be adopted along with RSSI to enhance the localization accuracy. Each of the reference point (RP) is presented by a group of CSI measurements from several WiFi subcarriers which we call CSI images. The combination of convolutional neural network (CNN) and LSTM model is proposed. CNN extracts the useful information from several CSI values (CSI images), and then LSTM will exploit this information in sequential timesteps to determine the user's location. Finally, a fully practical passive indoor localization is proposed. Most of the conventional methods rely on the collected WiFi signal on the mobile devices (active information), which requires a dedicated software to be installed. Different from them, we leverage the received data of the routers (passive information) to locate the position of the user. The localization accuracy is investigated through experiments with several phones, e.g., Nexus 5, Samsung, Iphone and HTC, in hundreds of testing locations. The experimental results demonstrate that our proposed localization scheme achieves an average localization error of around 1.5 m when the phone is in idle mode, and approximately 1 m when it actively transmits data.
Graduate
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cheng, chen-li, and 鄭振利. "Study of the bacterial community in a Triton X-100 bioredmediation system by 16S rDNA fingerprinting." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/70834767445816533355.

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碩士
國立中央大學
生命科學研究所
90
Triton X-100 is a non-ionic detergent and it often used in industrial, agricultural and household area. There are 5~85% surfactants mixing in the pesticides and herbicides. It can be directly introduced into the environment by spraying the pesticides as well as the herbicides through agricultural activities. The structure of the surfactant includes both hydrophilic and hydrophobic group. In addition to the toxicity, its chemical property also has great influence on the organic contaminants fates in the soil and even impacts public health. The focal point in this study is to use 16S ribosomal DNA fingerprinting method and denaturing gradient gel electrophoresis (DGGE) to analyze the community in the surfactant-polluted soil. Besides monitoring the relationship of community and Triton X-100, we added Pseudomonas sp. SH4 to see what changed in the community and Triton X-100 biodegradation. In this study, we used the microcosm which contained surfactant-like polluted soil over a long period of time to proceed with the research of surfactant bioremediation. There were 4.35 g Triton X—100 degrading in the SH4-added groups (72.5% of the original Triton X-100 weight). We chose the sample to proceed with 16S rDNA cloning library. Then we identified 11 strains by DGGE screening. From the phylogenetic analysis we knew that they were belong to the α, β, γ-proteobacteria. (except of the Flavobacterium sp. wuba 46) In the DGGE fingerprinting we found that Pseudomonas sp. SH4, Stenotrophomonas maltophilis, and Agrobacterium tumefaciens Zutra F/1 were dominant strains in the third group. Also, Pseudomonas sp. SH4, Stenotrophomonas maltophilia, Stenotrophomonas sp., and Stenotrophomonas maltophilia were dominant in the fourth, fifth, and sixth groups. In the group with SH4 and Triton X-100 but without air, we found that Agrobacterium tumefaciens Zutra F/1 and clone 4-70 (uncultured beta proteobacterium) are also dominant strains. Two microcosms contained Pseudomonas sp. SH4 and kept pumping air, but one of them was 30℃ fixed controlled. Two months later, 6 g Triton-100 were degraded completely. Whereas, the groups without SH4 degrader still contained about 2.67 g Trioton-100. Even three months later, 1.45 g Triton-100 would still be detected. As a result, Pseudomonas sp. SH4 plays an important role in the bioremediation.
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Abdennadher, Mourad. "Molecular fingerprinting, rDNA internal transcribed spacer sequence, and karyotype analysis of Ustilago hordei and related smut fungi." Thesis, 1995. http://hdl.handle.net/1957/34737.

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Inbreeding of the avirulent physiologic race 8 strains of Ustilago hordei was purported to have increased its pathogenicity in a stepwise manner that led to a highly pathogenic race, designated race 14. The analysis of electrophoretic karyotypes and restriction fragment length polymorphism profiles detected with a telomere-specific probe (TelomereRFLP) in races 8 and 14, and progeny obtained by selfing race 8 strains, revealed substantially changed patterns in the purported progeny of the second generation of selfing, and the race 14 strain. The telomereRFLP patterns in strains purported to be the progeny of the second generation of inbreeding of race 8, were unlike both race 8 and race 14 strains, and identical to those of U. hordei physiologic races 10 and 13. These data suggest that the progeny believed to have been derived from the second selfing of race 8 strains were clonal lineages from either race 10 or race 13 strains, rather than the products of meiosis of race 8 teliospores. The electrophoretic karyotypes resolved from race 8 and 14, revealed chromosome-length polymorphisms (CLPs) that were similar in magnitude to those reported among strains of the fourteen physiologic races of U. hordei, rejecting the postulate that race 14 is a lineage derivative from race 8. Electrophoretic karyotyping and ribosomal DNA (rDNA) sequence analysis of eight Ustilago species revealed that the four sporidium-forming species, U. avenae, U. hordei, U. kolleri, and U. nigra, form a coherent group. Ten probes detected a maximum of 15% CLPs among the putative homologous chromosomes of theses species, and the internal transcribed spacer (ITS) amplified from these species shared 97-99% sequence identity. The taxonomic distinctness of U. maydis from the rest of the smut fungi was evidenced by its divergence at 7 nucleotides in the 5.8S rDNA coding region.
Graduation date: 1996
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Martins, Cândida Filipa Gonçalves. "Caracterização fenotípica e genotípica de bactérias do ácido acético isoladas de alimentos." Master's thesis, 2014. http://hdl.handle.net/10348/3373.

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Dissertação de Mestrado em Biotecnologia e Qualidade Alimentar
As bactérias do ácido acético (BAA) são responsáveis pela produção de vários subprodutos muito utilizados na indústria alimentar. Podem atuar como agentes de transformação no caso do vinagre e como agentes de contaminação no caso do vinho. Neste trabalho, a resistência das bactérias acéticas a vários fatores de stress que ocorrem durante a produção industrial de vinagre, foi avaliada. Para tal, foram utilizadas 70 isolados provenientes de uvas sãs e podres, mostos, vinhos, sãos e alterados, e vinagres caseiros. A capacidade de resistência a diferentes condições de etanol, ácido acético, SO2 e pH e o efeito cruzado da temperatura foi avaliada por crescimento em meio de cultura sólidos. Foi observada uma grande variabilidade na resistência às diferentes condições testadas, sendo que a temperatura de incubação foi diretamente correlacionada com o aumento da sua sensibilidade. Foi observado que apenas um número reduzido destas bactérias demonstram capacidade para produzir aminas biogénicas nomeadamente a histamina (3%), e a putrescina (5%). Por outro lado, as bactérias acéticas estudadas revelaram um perfil de multiresistência a vários antibióticos o que sugere que estudos posteriores deverão ser conduzidos de forma a avaliar o seu impacto em termos de segurança alimentar. Com o objetivo de proceder à identificação dos isolados foram incluídas neste trabalho sete estirpes de referência, pertencentes à Colecção Espanhola de Culturas Tipo (CECT). A utilização da técnica de PCR fingerprinting com os primers M13, ERIC e (GTG)5 não permitiu obter a identificação credível dos isolados em estudo. Pela análise das sequências parciais do rDNA 16S foi possivel a identificação de 17 estirpes selecionadas aleatoriamente o que permitiu inferir a identificação de outros isolados. Assim, um grupo de estirpes identificados como Acetobacter pasteurianus foi caracterizado genotípicamente, tendo-se verificado uma grande variabilidade, também traduzida nos parâmetros fenotípicos avaliados.
Acetic acid bacteria (AAB) are responsible for the production of some interesting products, very useful for a variety of industries including food industry. They can have a positive role as transformation agents in vinegar production, but their growth is undesirable in the the wine industry being responsible for wine spoilage. In this work, the resistance of the acetic bacteria to some stress factors that they may encounter during production of vinegar, was evaluated. For such purpose, 70 acetic bacteria strains isolated from healthy and rotten grapes, grape-juices, wines, either spoiled or not, and vinegars, were used. The resistance of the isolates to different conditions of ethanol, acetic acid, SO2 and pH and the cross-effect of temperature was evaluated by its growth in solid culture media. A great variability in the resistance to the different tested conditions was observed, being temperature of incubation directly correlated with the increase of its sensitivity. Only a reduced number of these bacteria demonstrated capacity to produce biogenic amines, like histamine (3%) and putrescine (5%). On the other hand, acetic bacteria displayed an antibiotic multiresistance profile suggesting that further studies should be performed to assess its impact on food safety and public health. In order to identify the natural isolates used, seven reference strains belonging to the Spanish Type Culture Colection (CECT) were included in this work. PCR fingerprinting technique using M13, ERIC e (GTG)5 primers did not allow a reliable identification of the strains. Yet, 17 randomly selected strains were identified by analysis of the partial sequences of 16S rDNA which allowed us to infer the identification of some other isolates through the analysis of similarity of genotypic profiles obtained. The genotypic dissimilarity observed within the group of strains identified as Acetobacter pasteurianus was correlated with its divergence phenotypic profiles.
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Books on the topic "RNA fingerprinting"

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Peter, Bugert, ed. DNA and RNA profiling in human blood: Methods and protocols. New York, NY: Humana Press, 2009.

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Peng, Liang, Meade Jonathan D, and Pardee Arthur B. 1921-, eds. Differential display methods and protocols. 2nd ed. Totowa, N.J: Humana Press, 2005.

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Differential display methods and protocols. Totowa, N.J: Humana Press, 1997.

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Peng, Liang, and Pardee Arthur B. 1921-, eds. Differential display methods and protocols. Totowa, N.J: Humana Press, 1997.

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McClelland, Michael, ed. Expression Genetics: Differential Display (Biotechniques Update Series). EATON PUBLISHING, 1999.

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Pardee, Arthur B., Peng Liang, and Jonathan Meade. Differential Display Methods and Protocols. Humana Press, 2010.

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(Editor), Peng Liang, Jonathan Meade (Editor), and Arthur B. Pardee (Editor), eds. Differential Display Methods and Protocols (Methods in Molecular Biology). 2nd ed. Humana Press, 2005.

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Abdennadher, Mourad. Molecular fingerprinting, rDNA internal transcribed spacer sequence, and karyotype analysis of Ustilago hordei and related smut fungi. 1995.

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Book chapters on the topic "RNA fingerprinting"

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Stone, B. "Targeted RNA Fingerprinting." In Fingerprinting Methods Based on Arbitrarily Primed PCR, 371–87. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60441-6_38.

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Ricote, M., J. Welsh, and M. McClelland. "RNA Arbitrarily Primed PCR." In Fingerprinting Methods Based on Arbitrarily Primed PCR, 283–93. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60441-6_29.

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Bosch, T. C. G., and J. Lohmann. "Nonradioactive Differential Display of Messenger RNA." In Fingerprinting Methods Based on Arbitrarily Primed PCR, 295–304. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60441-6_30.

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Vaughan, Robert C., and C. Cheng Kao. "Mapping Protein–RNA Interactions by RCAP, RNA-Cross-Linking and Peptide Fingerprinting." In Methods in Molecular Biology, 225–36. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2562-9_16.

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McClelland, M., K. Chada, J. Welsh, and D. Ralph. "Arbitrary primed PCR fingerprinting of RNA applied to mapping differentially expressed genes." In DNA Fingerprinting: State of the Science, 103–15. Basel: Birkhäuser Basel, 1993. http://dx.doi.org/10.1007/978-3-0348-8583-6_10.

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Valinsky, Lea, Alexandra J. Scupham, Gianluca Delia Vedova, Zheng Liu, Andres Figueroa, Katechan Jampachaisri, Bei Yin, et al. "Section 3 update: Oligonucleotide Fingerprinting of Ribosomal RNA Genes (OFRG)." In Molecular Microbial Ecology Manual, 2471–87. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-2177-0_304.

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Mathieu-Daudé, Françoise, Karen Evans, Frank Kullmann, Rhonda Honeycutt, Thomas Vogt, John Welsh, and Michael McClelland. "Applications of DNA and RNA Fingerprinting by the Arbitrarily Primed Polymerase Chain Reaction." In Bacterial Genomes, 414–36. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-6369-3_33.

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Yacobi, Yacov. "Improved Boneh-Shaw Content Fingerprinting." In Topics in Cryptology — CT-RSA 2001, 378–91. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/3-540-45353-9_28.

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Gellert, Kai, Tibor Jager, Lin Lyu, and Tom Neuschulten. "On Fingerprinting Attacks and Length-Hiding Encryption." In Topics in Cryptology – CT-RSA 2022, 345–69. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-95312-6_15.

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Fang, Zhenghan, Yong Chen, Dong Nie, Weili Lin, and Dinggang Shen. "RCA-U-Net: Residual Channel Attention U-Net for Fast Tissue Quantification in Magnetic Resonance Fingerprinting." In Lecture Notes in Computer Science, 101–9. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-32248-9_12.

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Conference papers on the topic "RNA fingerprinting"

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Zou, Pengfei, Ang Li, Kevin Barker, and Rong Ge. "Fingerprinting Anomalous Computation with RNN for GPU-accelerated HPC Machines*." In 2019 IEEE International Symposium on Workload Characterization (IISWC). IEEE, 2019. http://dx.doi.org/10.1109/iiswc47752.2019.9042165.

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Kromer-Edwards, Cory, Mariana Castanheira, and Suely Oliveira. "K-Mer Fingerprinting with RNN to predict MICs for K. pneumoniae." In 2022 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2022. http://dx.doi.org/10.1109/bibm55620.2022.9995374.

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