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Relevant bibliographies by topics / RNA-DNA FISH

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Academic literature on the topic 'RNA-DNA FISH'

Author: Grafiati

Published: 25 May 2024

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Journal articles on the topic "RNA-DNA FISH"

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Greenberg, Eliraz, Hodaya Hochberg-Laufer, Shalev Blanga, Noa Kinor, and Yaron Shav-Tal. "Cytoplasmic DNA can be detected by RNA fluorescence in situ hybridization." Nucleic Acids Research 47, no. 18 (2019): e109-e109. http://dx.doi.org/10.1093/nar/gkz645.

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Abstract Fluorescence in situ hybridization (FISH) can be used for the intracellular detection of DNA or RNA molecules. The detection of DNA sequences by DNA FISH requires the denaturation of the DNA double helix to allow the hybridization of the fluorescent probe with DNA in a single stranded form. These hybridization conditions require high temperature and low pH that can damage RNA, and therefore RNA is not typically detectable by DNA FISH. In contrast, RNA FISH does not require a denaturation step since RNA is single stranded, and therefore DNA molecules are not detectable by RNA FISH. Hen

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Ferguson, Moira M., and Roy G. Danzmann. "RNA/DNA ratios in white muscle as estimates of growth in rainbow trout held at different temperatures." Canadian Journal of Zoology 68, no. 7 (1990): 1494–98. http://dx.doi.org/10.1139/z90-221.

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The concentrations of RNA, DNA, and protein in white muscle from 240 uniquely tagged rainbow trout (Oncorhynchus mykiss) held at three temperatures (5, 8 (control), and 11 °C) were measured. Both RNA and RNA/DNA ratios were better predictors of recent length- and weight-specific growth rates than they were of absolute fish size. Furthermore, RNA concentrations were better predictors of growth than RNA/DNA ratios. The strength of the regression between either RNA/DNA ratio or RNA and growth rate did not differ consistently among temperatures. Fish reared at warmer temperatures had lower concent

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Lai, Lan-Tian, Pin Jie Lee, and Li-Feng Zhang. "Immunofluorescence protects RNA signals in simultaneous RNA–DNA FISH." Experimental Cell Research 319, no. 3 (2013): 46–55. http://dx.doi.org/10.1016/j.yexcr.2012.11.009.

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Labh, Shyam Narayan. "RNA: DNA Ratio and Growth Performance of Rohu Labeo rohita (Hamilton) Fed Varied Proportion of Protein Diet during Intensive Aquaculture." International Journal of Life Sciences 9, no. 6 (2015): 113–22. http://dx.doi.org/10.3126/ijls.v9i6.11585.

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An experiment was conducted at Corona of Agriculture (COA)”, Gunjanagar-3, Chitwan, Nepal to complete a project granted by University Grant Commission (UGC), Second Higher Education Project (SHEP) in 2013. Four (average 380 m2) ponds (W, X, Y and Z) with four different diets as D1 (20%), D2 (30%), D3 (40%) and D4 (50%) protein contents were used to conduct the experiment properly to determine the varied proportion of dietary protein on the growth performance of major carp rohu Labeo Rohita (H) in relation with RNA: DNA ratio. After 12th weeks of culture, average length, average weight and spec

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Yan, Zhangming, Norman Huang, Weixin Wu, et al. "Genome-wide colocalization of RNA–DNA interactions and fusion RNA pairs." Proceedings of the National Academy of Sciences 116, no. 8 (2019): 3328–37. http://dx.doi.org/10.1073/pnas.1819788116.

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Fusion transcripts are used as biomarkers in companion diagnoses. Although more than 15,000 fusion RNAs have been identified from diverse cancer types, few common features have been reported. Here, we compared 16,410 fusion transcripts detected in cancer (from a published cohort of 9,966 tumor samples of 33 cancer types) with genome-wide RNA–DNA interactions mapped in two normal, noncancerous cell types [using iMARGI, an enhanced version of the mapping of RNA–genome interactions (MARGI) assay]. Among the top 10 most significant RNA–DNA interactions in normal cells, 5 colocalized with the gene

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Ogata, Motoyuki, Gosuke Hayashi, Anri Ichiu, and Akimitsu Okamoto. "l-DNA-tagged fluorescence in situ hybridization for highly sensitive imaging of RNAs in single cells." Organic & Biomolecular Chemistry 18, no. 40 (2020): 8084–88. http://dx.doi.org/10.1039/d0ob01635g.

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l-DNA tagged FISH (LT-FISH), including two-step hybridization processes with a l–d chimera oligonucleotide and a fluorescence-labeled PCR product tethering a l-DNA tag, has realized sensitive RNA detection in fixed cells.

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Chen, Feng, Min Bai, Xiaowen Cao, Yue Zhao, Jing Xue, and Yongxi Zhao. "Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures." Nucleic Acids Research 47, no. 22 (2019): e145-e145. http://dx.doi.org/10.1093/nar/gkz852.

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Abstract Spatially resolved visualization of RNA processing and structures is important for better studying single-cell RNA function and landscape. However, currently available RNA imaging methods are limited to sequence analysis, and not capable of identifying RNA processing events and structures. Here, we developed click-encoded rolling FISH (ClickerFISH) for visualizing RNA polyadenylation and structures in single cells. In ClickerFISH, RNA 3′ polyadenylation tails, single-stranded and duplex regions are chemically labeled with different clickable DNA barcodes. These barcodes then initiate

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Staeheli, P., Y. X. Yu, R. Grob, and O. Haller. "A double-stranded RNA-inducible fish gene homologous to the murine influenza virus resistance gene Mx." Molecular and Cellular Biology 9, no. 7 (1989): 3117–21. http://dx.doi.org/10.1128/mcb.9.7.3117-3121.1989.

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We cloned and sequenced a 2.35-kilobase EcoRI fragment of genomic DNA from a local freshwater fish (Perca fluviatilis) that strongly hybridized to probes derived from the murine influenza virus resistance gene Mx. The cloned fish DNA contained blocks of sequences related to Mx gene exons 3 to 8, which appeared to represent exons of a bona fide fish gene because they were separated by intron sequences flanked by consensus splice acceptor and donor sites. Injection of double-stranded RNA into the peritoneal cavity of trouts resulted in 5- to 10-fold elevated levels of two liver mRNAs of about 2.

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Ravikiran, K., and R. S. Kulkarni. "Nucleic Acid Content in Male Fresh Water Fish N. notopterus Exposed to Copper Sulphate." International Letters of Natural Sciences 33 (January 2015): 1–8. http://dx.doi.org/10.18052/www.scipress.com/ilns.33.1.

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The nucleic acid content in different tissues such as brain, liver, kidney & testis of both control and copper sulphate (CuSO4) exposed freshwater fish N. notopterus has been studied. The following observation is made in both control and copper sulphate (CuSO4) exposed fish. The testis contain large amount of DNA in comparison to other tissues. The degree of DNA content in control and copper sulphate (CuSO4) exposed fish testis >liver>brain>kidney. The RNA content also exhibited similar to that of DNA, having higher amount in the testis. The degree of RNA content in control and co

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Staeheli, P., Y. X. Yu, R. Grob, and O. Haller. "A double-stranded RNA-inducible fish gene homologous to the murine influenza virus resistance gene Mx." Molecular and Cellular Biology 9, no. 7 (1989): 3117–21. http://dx.doi.org/10.1128/mcb.9.7.3117.

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We cloned and sequenced a 2.35-kilobase EcoRI fragment of genomic DNA from a local freshwater fish (Perca fluviatilis) that strongly hybridized to probes derived from the murine influenza virus resistance gene Mx. The cloned fish DNA contained blocks of sequences related to Mx gene exons 3 to 8, which appeared to represent exons of a bona fide fish gene because they were separated by intron sequences flanked by consensus splice acceptor and donor sites. Injection of double-stranded RNA into the peritoneal cavity of trouts resulted in 5- to 10-fold elevated levels of two liver mRNAs of about 2.

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Dissertations / Theses on the topic "RNA-DNA FISH"

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Costa, Filho João. "Relações da taxa RNA/DNA e parâmetros morfológicos no crescimento de juvenis de robalo-flecha (Centropomus undecimalis) cultivados." Universidade do Estado de Santa Catarina, 2013. http://tede.udesc.br/handle/handle/869.

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Made available in DSpace on 2016-12-08T16:24:12Z (GMT). No. of bitstreams: 1 PGCA13MA092.pdf: 472096 bytes, checksum: aa4f745bb7f62d5ae8cd6ad77d0a4c7d (MD5) Previous issue date: 2013-07-09<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>The marine fish farming and culture of snook, Centropomus undecimalis, have good prospects for commercial development in Brazil. In this regard, there is a need to expand studies on the evaluation of growth on snook, which can be realized by morphological or biochemical methods. The nutritional status and growth of fish can be influenced by

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Gimenez, Octavio Manuel Palacios [UNESP]. "Padrões de evolução de sistemas de cromossomos sexuais em grilos: uma abordagem integrada entre citogenética e genômica." Universidade Estadual Paulista (UNESP), 2017. http://hdl.handle.net/11449/152458.

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Papucci, Chiara. "Study of position effect as a mechanism arising from chromosomal translocations in leukaemia." Thesis, Brunel University, 2015. http://bura.brunel.ac.uk/handle/2438/11583.

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The chromosomal translocation of t(14;18)(14q32;18q21) is a characteristic aberration of follicular lymphoma and Diffuse Large B cells lymphoma. By PCR, it was proved that the rearrangement of chromosomes 14 and 18 leads to an overexpression of BCL2, an anti-apoptotic protein, which is one of the factors responsible for the maturation of the diseases. The translocation involves the promoter region of IGH gene and the transcriptional unit of BCL2 gene. Previous studies carried out in Dr Tosi’s lab showed a looping out of the BCL2 gene from its chromosome territory in 15% of the nuclei analysed.

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Kerlin, Maciej. "Gene coregulation in cis within the 3D genome – A single-molecule imaging study." Electronic Thesis or Diss., Sorbonne université, 2021. http://www.theses.fr/2021SORUS124.

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Le génome eucaryote est organisé dans l’espace et le long de sa séquence. Du chromosome aux gènes, son organisation 3D est étroitement liée à son activité transcriptionnelle. A une échelle inférieure à la mégabase, le génome est organisé physiquement en domaines d’auto-interaction (TAD, pour topologically associating domains), que l’on pense influencer l’action de séquences régulatrices des gènes, appelées ‘amplificateurs’. Les TAD sont parfois vus comme des ‘unités de régulation’ permettant de coréguler plusieurs gènes en les exposant aux mêmes amplificateurs. L’expression de gènes d’un même

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Adamson, Eleanor A. S. "Influence of historical landscapes, drainage evolution and ecological traits on patterns of genetic diversity in Southeast Asian freshwater snakehead fishes." Thesis, Queensland University of Technology, 2010. https://eprints.qut.edu.au/39612/1/Eleanor_Adamson_Thesis.pdf.

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Snakehead fishes in the family Channidae are obligate freshwater fishes represented by two extant genera, the African Parachannna and the Asian Channa. These species prefer still or slow flowing water bodies, where they are top predators that exercise high levels of parental care, have the ability to breathe air, can tolerate poor water quality, and interestingly, can aestivate or traverse terrestrial habitat in response to seasonal changes in freshwater habitat availability. These attributes suggest that snakehead fishes may possess high dispersal potential, irrespective of the terrestrial ba

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Trovato, Fabio. "Molecular Dynamics Simulations of biopolymers within the cell environment: Minimalist models for the Nucleic Acids and Green Fluorescent Proteins in the cytoplasm." Doctoral thesis, Scuola Normale Superiore, 2013. http://hdl.handle.net/11384/85896.

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From the introduction: "... This thesis reports on simplified models (`coarse grained') of proteins and nucleic acids in which many degrees of freedom (DOF) are eliminated, to speed up the computation of the interactions. The resolution of these models is minimal, with respect to the representation of the phenomena studied, in order to extend the time and length scales accessible to atomistic simulations. Furthermore the existence of a coarse- to fine-grain backmapping ensures to switch between diff erent resolutions of the same system, naturally leading to an integrative approach used

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Fox, Samuel E. "Transcriptomic analysis using high-throughput sequencing and DNA microarrays." Thesis, 2011. http://hdl.handle.net/1957/23741.

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Transcriptomics and gene expression profiling enables the elucidation of the genetic response of an organism to various environmental cues. Transcriptomics enables the deciphering of differences between two closely related organisms to the same environment and in contrast, enables the elucidation of genetic responses of the same organism to different environmental cues. Two major methods are utilized for the study of transcriptomes, high-throughput sequencing and microarray analysis. High-throughput sequencing technologies such as the Illumina platform are relatively new and protocols must be

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Book chapters on the topic "RNA-DNA FISH"

1

Lai, Lan-Tian, Zhenyu Meng, Fangwei Shao, and Li-Feng Zhang. "Simultaneous RNA–DNA FISH." In Long Non-Coding RNAs. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3378-5_11.

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Lai, Lan-Tian, Zhenyu Meng, Fangwei Shao, and Li-Feng Zhang. "Simultaneous RNA-DNA FISH." In Long Non-Coding RNAs. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1697-0_11.

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Guioli, Silvana, and Robin Lovell-Badge. "RNA FISH, DNA FISH and Chromosome Painting of Chicken Oocytes." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3795-0_14.

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Sen, Souvik, Shivnarayan Dhuppar, and Aprotim Mazumder. "Combined 3D DNA FISH, Single-Molecule RNA FISH, and Immunofluorescence." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3766-1_14.

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Magaraki, Aristea, Agnese Loda, Joost Gribnau, and Willy M. Baarends. "Simultaneous RNA–DNA FISH in Mouse Preimplantation Embryos." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8766-5_11.

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Okamoto, Ikuhiro. "Combined Immunofluorescence, RNA FISH, and DNA FISH in Preimplantation Mouse Embryos." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8766-5_12.

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Pei, Lei, and Markus Schmidt. "Containment strategies for synthetic gene drive organisms and impacts on gene flow." In Gene flow: monitoring, modeling and mitigation. CABI, 2021. http://dx.doi.org/10.1079/9781789247480.0010.

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Abstract Gene drives, particularly synthetic gene drives, may help to address some important challenges, by efficiently altering specific sections of DNA in entire populations of wild organisms. Here we review the current development of the synthetic gene drives, especially those RNA-guided synthetic gene drives based on the CRISPR nuclease Cas. Particular focuses are on their possible applications in agriculture (e.g. disease resistance, weed control management), ecosystem conservation (e.g. evasion species control), health (e.g. to combat insect-borne and fungal infections), and for basic research in model organisms (e.g. Saccharomyces, fruit fly, and zebra fish). The physical, chemical, biological, and ecological containment strategies that might help to confine these gene drive-modified organisms are then explored. The gene flow issues, those from gene drive-derived organisms to the environment, are discussed, while possible mitigation strategies for gene drive research are explored. Last but not least, the regulatory context and opinions from key stakeholders (regulators, scientists, and concerned organizations) are reviewed, aiming to provide a more comprehensive overview of the field.

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Pollex, Tim, Tristan Piolot, and Edith Heard. "Live-Cell Imaging Combined with Immunofluorescence, RNA, or DNA FISH to Study the Nuclear Dynamics and Expression of the X-Inactivation Center." In Imaging Gene Expression. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-526-2_2.

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Solovei, I., J. Walter, M. Cremer, F. Habermann, L. Schermelleh, and T. Cremer. "FISH on three-dimensionally preserved nuclei." In Fish. Oxford University PressOxford, 2002. http://dx.doi.org/10.1093/oso/9780199638833.003.0007.

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Abstract This chapter focuses on the fluorescence in situ hybridization (FISH) of DNA probes to three-dimensionally preserved cells, so-called 3D FISH. This technique allows three-dimensional visualization of specific DNA and RNA targets within the nucleus at all stages of the cell cycle. It provides information about the arrangement of chromosome territories and the organization of sub chromosomal domains, about the pattern of chromatin density within a chromosome territory, about positions of individual genes and RNA transcripts read from them. Accumulation of such data is necessary for understanding relationships between the spatial organization of the genome and its functioning in the interphase nucleus.

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Wang, Qiuyu, Nessar Ahmed, and Chris Smith. "Molecular biology techniques." In Biomedical Science Practice. Oxford University Press, 2022. http://dx.doi.org/10.1093/hesc/9780198831228.003.0014.

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This chapter examines molecular biology, which is generally concerned with the structures, functions, and interactions of the two major groups of macromolecules, the nucleic acids DNA and RNA, and proteins. Nucleic acids can be purified from organisms using a variety of techniques. Restriction endonucleases (REs) can digest the isolated DNA into smaller-sized fragments suitable for analysis and for use in a number of techniques of clinical interest. Molecular biology techniques are used in biomedical science and they rely on the complementary binding of nucleic acids to provide a convenient way of recognizing and isolating specific base sequences within fragments of DNA or RNA molecules include the sequencing of isolated DNA, Southern and Northern blotting, fluorescence in situ hybridization (FISH), the cloning of DNA by recombination and polymerase chain reaction (PCR) technologies, and DNA microarray analysis. The chapter then looks at CRISPR–Cas9, which is a quick and efficient method for editing genes.

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