Journal articles on the topic 'RLF'
To see the other types of publications on this topic, follow the link: RLF.
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'RLF.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Rudder, TH, GW Seifert, and HM Burrow. "Environmental and genotype effects on fertility in a commercial beef herd in central Queensland." Australian Journal of Experimental Agriculture 25, no. 3 (1985): 489. http://dx.doi.org/10.1071/ea9850489.
Full textAbstract:
Joining liveweight, proportion pregnant and calving date were recorded from relatively low fertility (RLF) and relatively high fertility (RHF) tropically adapted genotypes. RLF genotypes were F2 et seq. Brahman x British crossbreds and Brahman back crosses while RHF genotypes were F1 Brahman x British and Africander infused crossbreds. Year effects had the largest and most consistent influence on joining liveweight, proportion of cows pregnant and calving date. RLF genotypes had a lower proportion pregnant than RHF genotypes in yearling heifers, 2-5-year-old and >9- year-old lactating cows, but there was no difference in the 6-8-year age group. Calving dates tended to be later in the RLF genotypes in the 2-5-year age groups, but were the same as RHF genotypes in older age groups. Annual variation in joining liveweight was explained by May-October rainfall, which was an indication of pasture productivity levels. The proportion of cows pregnant was related to joining liveweight; to achieve a high proportion in the RHF genotypes, target joining liveweights of about 275 and 375 kg were indicated for yearling heifers and lactating cows, respectively. There was no difference between RLF and RHF in the 6-8-year lactating age group, but in other age groups RLF genotypes tended to require an extra 25-75 kg joining liveweight to approximate the proportion pregnant in RHF genotypes over the lower and mid-liveweight ranges. However, at highest joining liveweights, RLF had a lower proportion pregnant than did RHF genotypes. Calving date influenced the proportion that became pregnant during the subsequent joining season, young cows being more sensitive to calving date than old cows, and RLF more sensitive than RHF genotypes.
2
Mäkelä, T. P., J. Kere, R. Winqvist, and K. Alitalo. "Intrachromosomal rearrangements fusing L-myc and rlf in small-cell lung cancer." Molecular and Cellular Biology 11, no. 8 (August 1991): 4015–21. http://dx.doi.org/10.1128/mcb.11.8.4015.
Full textAbstract:
Chromosomal abnormalities affecting proto-oncogenes are frequently detected in human cancer. Oncogenes of the myc family are activated in several types of tumors as a result of gene amplification or chromosomal translocation. We have recently found the L-myc gene involved in a gene fusion in small-cell lung cancer (SCLC). This results in a chimeric protein with amino-terminal sequences from a novel gene named rif joined to L-myc. Here we present a preliminary structural characterization of the rlf-L-myc fusion gene, which has been found only in cells with an amplified L-myc gene. In addition, we have used somatic cell hybrids to assign the normal rlf locus to the same chromosome (chromosome 1) on which L-myc resides. Finally, we have been able to establish a physical linkage between rif and L-myc with pulsed-field gel electrophoresis. Our results demonstrate that normal rlf and L-myc genes are separated by less than 800 kb of DNA. Thus, the rlf-L-myc gene fusions are due to similar but not identical intrachromosomal rearrangements at 1p32. The presence of independent genetic lesions that cause the formation of identical chimeric rlf-L-myc proteins suggests a role for the fusion protein in the development of these tumors.
3
Mäkelä, T. P., J. Kere, R. Winqvist, and K. Alitalo. "Intrachromosomal rearrangements fusing L-myc and rlf in small-cell lung cancer." Molecular and Cellular Biology 11, no. 8 (August 1991): 4015–21. http://dx.doi.org/10.1128/mcb.11.8.4015-4021.1991.
Full textAbstract:
Chromosomal abnormalities affecting proto-oncogenes are frequently detected in human cancer. Oncogenes of the myc family are activated in several types of tumors as a result of gene amplification or chromosomal translocation. We have recently found the L-myc gene involved in a gene fusion in small-cell lung cancer (SCLC). This results in a chimeric protein with amino-terminal sequences from a novel gene named rif joined to L-myc. Here we present a preliminary structural characterization of the rlf-L-myc fusion gene, which has been found only in cells with an amplified L-myc gene. In addition, we have used somatic cell hybrids to assign the normal rlf locus to the same chromosome (chromosome 1) on which L-myc resides. Finally, we have been able to establish a physical linkage between rif and L-myc with pulsed-field gel electrophoresis. Our results demonstrate that normal rlf and L-myc genes are separated by less than 800 kb of DNA. Thus, the rlf-L-myc gene fusions are due to similar but not identical intrachromosomal rearrangements at 1p32. The presence of independent genetic lesions that cause the formation of identical chimeric rlf-L-myc proteins suggests a role for the fusion protein in the development of these tumors.
4
Mahbubani, Hiro M., James P. J. Chong, Stephane Chevalier, Pia Thömmes, and J. Julian Blow. "Cell Cycle Regulation of the Replication Licensing System: Involvement of a Cdk-dependent Inhibitor." Journal of Cell Biology 136, no. 1 (January 13, 1997): 125–35. http://dx.doi.org/10.1083/jcb.136.1.125.
Full textAbstract:
The replication licensing factor (RLF) is an essential initiation factor that is involved in preventing re-replication of chromosomal DNA in a single cell cycle. In Xenopus egg extracts, it can be separated into two components: RLF-M, a complex of MCM/P1 polypeptides, and RLF-B, which is currently unpurified. In this paper we investigate variations in RLF activity throughout the cell cycle. Total RLF activity is low in metaphase, due to a lack of RLF-B activity and the presence of an RLF inhibitor. RLF-B is rapidly activated on exit from metaphase, and then declines during interphase. The RLF inhibitor present in metaphase extracts is dependent on the activity of cyclin-dependent kinases (Cdks). Affinity depletion of Cdks from metaphase extracts removed the RLF inhibitor, while Cdc2/cyclin B directly inhibited RLF activity. In metaphase extracts treated with the protein kinase inhibitor 6-dimethylaminopurine (6-DMAP), both cyclin B and the RLF inhibitor were stabilized although the extracts morphologically entered interphase. These results are consistent with studies in other organisms that invoke a key role for Cdks in preventing re-replication of DNA in a single cell cycle.
5
Lu, Hang, Jason Catania, Katalin Baranji, Jie Feng, Mili Gu, Janet Lathey, Diane Sweeny, et al. "Characterization of the Native Form of Anthrax Lethal Factor for Use in the Toxin Neutralization Assay." Clinical and Vaccine Immunology 20, no. 7 (May 1, 2013): 986–97. http://dx.doi.org/10.1128/cvi.00046-13.
Full textAbstract:
ABSTRACTThe cell-based anthrax toxin neutralization assay (TNA) is used to determine functional antibody titers of sera from animals and humans immunized with anthrax vaccines. The anthrax lethal toxin is a critical reagent of the TNA composed of protective antigen (PA) and lethal factor (LF), which are neutralization targets of serum antibodies. Cytotoxic potency of recombinant LF (rLF) lots can vary substantially, causing a challenge in producing a renewable supply of this reagent for validated TNAs. To address this issue, we characterized a more potent rLF variant (rLF-A) with the exact native LF amino acid sequence that lacks the additional N-terminal histidine and methionine residues present on the commonly used form of rLF (rLF-HMA) as a consequence of the expression vector. rLF-A can be used at 4 to 6 ng/ml (in contrast to 40 ng/ml rLF-HMA) with 50 ng/ml recombinant PA (rPA) to achieve 95 to 99% cytotoxicity. In the presence of 50 ng/ml rPA, both rLF-A and rLF-HMA allowed for similar potencies (50% effective dilution) among immune sera in the TNA. rPA, but not rLF, was the dominant factor in determining potency of serum samples containing anti-PA antibodies only or an excess of anti-PA relative to anti-rLF antibodies. Such anti-PA content is reflected in immune sera derived from most anthrax vaccines in development. These results support that 7- to 10-fold less rLF-A can be used in place of rLF-HMA without changing TNA serum dilution curve parameters, thus extending the use of a single rLF lot and a consistent, renewable supply.
6
Ramm, G. A., R. S. Britton, R. O'Neill, H. D. Kohn, and B. R. Bacon. "Rat liver ferritin selectively inhibits expression of alpha-smooth muscle actin in cultured rat lipocytes." American Journal of Physiology-Gastrointestinal and Liver Physiology 270, no. 2 (February 1, 1996): G370—G375. http://dx.doi.org/10.1152/ajpgi.1996.270.2.g370.
Full textAbstract:
The role of ferritin in lipocyte activation is unknown. This study examined the effect of rat liver ferritin (RLF), human recombinant H-ferritin (HrHF), human recombinant L-ferritin (HrLF), apo-ferritin (apo-RLF), and hemin on lipocyte activation. Lipocytes were cultured on uncoated plastic and were incubated with these agents for 7 days, at concentrations ranging from 10(-14) to 10(-7) M (0.5 to 50 microM for hemin). Collagen/noncollagen protein production and lipocyte proliferation were determined by [3H]proline and [3H]thymidine incorporation, respectively, and the expression of alpha-smooth muscle actin (alpha-SMA) and desmin was determined by Western blot. RLF, at concentrations ranging from 10(-10) to 10(-7) M, decreased alpha-SMA expression by 65-88%. Apo-RLF, HrHF, and HrLF decreased alpha-SMA by 17-45% at 10(-7) and 10(-8) M. Hemin (10 or 50 microM) inhibited alpha-SMA by 37 and 54%, respectively. Desmin expression was not altered by ferritin or hemin. Collagen and noncollagen protein production were not altered by either RLF or apo-RLF. Lipocyte proliferation was decreased by 54, 32, and 40%, by 10(-7) M RLF, HrHF, and HrLF, respectively, whereas apo-RLF had no effect. Thus RLF inhibited lipocyte alpha-SMA expression, which may be due to an effect of sequestered iron, since neither apo-RLF, HrHF, nor HrLF had a potent effect on alpha-SMA expression and all are essentially iron-free. The inhibitory effect of iron-loaded RLF on alpha-SMA expression suggests that tissue ferritin does not initiate lipocyte activation in iron overload, but rather may have a suppressive action on this process.
7
Minagawa, Itaru, Masafumi Fukuda, Hisako Ishige, Hiroshi Kohriki, Masatoshi Shibata, Enoch Y. Park, Tatsuo Kawarasaki, and Tetsuya Kohsaka. "Relaxin-like factor (RLF)/insulin-like peptide 3 (INSL3) is secreted from testicular Leydig cells as a monomeric protein comprising three domains B–C–A with full biological activity in boars." Biochemical Journal 441, no. 1 (December 14, 2011): 265–73. http://dx.doi.org/10.1042/bj20111107.
Full textAbstract:
RLF (relaxin-like factor), also known as INSL3 (insulin-like peptide 3), is a novel member of the relaxin/insulin gene family that is expressed in testicular Leydig cells. Despite the implicated role of RLF/INSL3 in testis development, its native conformation remains unknown. In the present paper we demonstrate for the first time that boar testicular RLF/INSL3 is isolated as a monomeric structure with full biological activity. Using a series of chromatography steps, the native RLF/INSL3 was highly purified as a single peak in reverse-phase HPLC. MS/MS (tandem MS) analysis of the trypsinized sample provided 66% sequence coverage and revealed a distinct monomeric structure consisting of the B-, C- and A-domains deduced previously from the RLF/INSL3 cDNA. Moreover, the N-terminal peptide was four amino acid residues longer than predicted previously. MS analysis of the intact molecule and PMF (peptide mass fingerprinting) analysis at 100% sequence coverage confirmed this structure and indicated the existence of three site-specific disulfide bonds. RLF/INSL3 retained full bioactivity in HEK (human embryonic kidney)-293 cells expressing RXFP2 (relaxin/insulin-like family peptide receptor 2), the receptor for RLF/INSL3. Furthermore, RLF/INSL3 was found to be secreted from Leydig cells into testicular venous blood. Collectively, these results indicate that boar RLF/INSL3 is secreted from testicular Leydig cells as a B–C–A monomeric structure with full biological activity.
8
Mutha, R. E., D. M. Dhumal, P. H. Patil, A. A. Shirkhedkar, and S. B. Bari. "QUANTITATIVE DETERMINATION OF RALOXIFENE HYDROCHLORIDE IN BULK AND TABLET FORMULATION BY UV-SPECTROPHOTOMETRY AND FIRST ORDER DERIVATIVE USING AREA UNDER CURVE METHOD." INDIAN DRUGS 49, no. 09 (September 28, 2012): 40–43. http://dx.doi.org/10.53879/id.49.09.p0040.
Full textAbstract:
Raloxifene hydrochloride (RLF) is a non-steroidal, selective estrogen receptor modulator used in the treatment and prevention of osteoporosis in postmenopausal women. Two simple, rapid and economical 'zero order UV-spectrophotometric (method I)' and first order derivative spectrophotometric (method II) using area under curve (AUC) technique have been developed for estimation of RLF in bulk and tablets. In 0.1N NaOH, RLF showed maximum absorbance at 303.5 nm. In method I, AUC between two wavelengths 275.0 nm & 350.0 nm were selected. In method II, UV-spectrum of RLF was derivatized into first order and two wavelengths 303.0 nm and 385.0 nm were selected for determination of AUC. In both these methods, RLF obeyed linearity in the concentration range of 2-10 mcg/mL (r2>0.99). Proposed methods were applied for tablet formulations and amounts of RLF estimated by method I and method II were found to be 100.08±0.68 and 99.78±0.94, respectively. Both these methods were validated statistically and by recovery experiments.
9
Eckley, Claudia A., and Henrique O. Costa. "Estudo da concentração salivar do fator de crescimento epidérmico em indivíduos com laringite crônica por refluxo laringofaríngeo." Revista Brasileira de Otorrinolaringologia 69, no. 5 (October 2003): 590–97. http://dx.doi.org/10.1590/s0034-72992003000500002.
Full textAbstract:
INTRODUÇÃO: A Doença do Refluxo Gastroesofágico (DRGE), chegando até a faringe e laringe, pode cursar com intensa inflamação local e rica sintomatologia (Refluxo Laringofaríngeo RLF). Os estudos atuais não foram capazes de provar que o ácido refluído é o causador das alterações visualizadas na laringite crônica. O Fator de Crescimento Epidérmico (EGF) é um polipeptídeo produzido pelas glândulas salivares, sendo implicado na indução do crescimento epitelial, na inibição da secreção gástrica e na aceleração da cicatrização. Deficiência salivar deste fator foi encontrada na esofagite de refluxo, mas não há relatos sobre a concentração salivar de EGF em indivíduos com RLF. OBJETIVO: Determinar a concentração salivar do EGF em indivíduos com RLF. FORMA DE ESTUDO: Caso controle. CASUÍSTICA E MÉTODO: A concentração salivar de EGF de 39 indivíduos com RLF e 20 controles normais foi estudada pela técnica de ELISA. O RLF foi diagnosticado por história e exame videolaringoscópico característicos. Os 39 pacientes com RLF foram estratificados de acordo com os achados de endoscopia digestiva (com ou sem esofagite associada) e de acordo com a intensidade da laringite crônica. Foram, também, submetidos a manometria esofágica e pH-metria esofágica de 24 horas com dois canais. RESULTADOS: Constatou-se uma concentração de EGF significativamente menor nos indivíduos com RLF quando comparados aos controles normais (p=0,002). Não houve diferença estatisticamente significante na concentração salivar de EGF entre os indivíduos com RLF, nem em relação à presença de esofagite, nem quanto à intensidade da laringite. CONCLUSÕES: Este estudo sugere que uma deficiência na concentração salivar do Fator de Crescimento Epidérmico pode estar associada à patogenia da DRGE, e que este polipeptídeo poderia ser um co-fator na gênese do RLF.
10
Bamberger, Ana-Maria, Richard Ivell, Marga Balvers, Bianca Kelp, Christoph M. Bamberger, Lutz Riethdorf, and Thomas Löning. "Relaxin-Like Factor (RLF)." International Journal of Gynecological Pathology 18, no. 2 (April 1999): 163–68. http://dx.doi.org/10.1097/00004347-199904000-00011.
Full text
11
Nguyen, Melissa L., Simon Terzyan, Jimmy D. Ballard, Judith A. James, and A. Darise Farris. "The Major Neutralizing Antibody Responses to Recombinant Anthrax Lethal and Edema Factors Are Directed to Non-Cross-Reactive Epitopes." Infection and Immunity 77, no. 11 (August 31, 2009): 4714–23. http://dx.doi.org/10.1128/iai.00749-09.
Full textAbstract:
ABSTRACT Anthrax lethal and edema toxins (LeTx and EdTx, respectively) form by binding of lethal factor (LF) or edema factor (EF) to the pore-forming moiety protective antigen (PA). Immunity to LF and EF protects animals from anthrax spore challenge and neutralizes anthrax toxins. The goal of the present study is to identify linear B-cell epitopes of EF and to determine the relative contributions of cross-reactive antibodies of EF and LF to LeTx and EdTx neutralization. A/J mice were immunized with recombinant LF (rLF) or rEF. Pools of LF or EF immune sera were tested for reactivity to rLF or rEF by enzyme-linked immunosorbent assays, in vitro neutralization of LeTx and EdTx, and binding to solid-phase LF and EF decapeptides. Cross-reactive antibodies were isolated by column absorption of EF-binding antibodies from LF immune sera and by column absorption of LF-binding antibodies from EF immune sera. The resulting fractions were subjected to the same assays. Major cross-reactive epitopes were identified as EF amino acids (aa) 257 to 268 and LF aa 265 to 274. Whole LF and EF immune sera neutralized LeTx and EdTx, respectively. However, LF sera did not neutralize EdTx, nor did EF sera neutralize LeTx. Purified cross-reactive immunoglobulin G also failed to cross-neutralize. Cross-reactive B-cell epitopes in the PA-binding domains of whole rLF and rEF occur and have been identified; however, the major anthrax toxin-neutralizing humoral responses to these antigens are constituted by non-cross-reactive epitopes. This work increases understanding of the immunogenicity of EF and LF and offers perspective for the development of new strategies for vaccination against anthrax.
12
Büllesbach, Erika E., Fredric R. Boockfor, George Fullbright, and Christian Schwabe. "Cryptorchidism induced in normal rats by the relaxin-like factor inhibitor." REPRODUCTION 135, no. 3 (March 2008): 351–55. http://dx.doi.org/10.1530/rep-07-0330.
Full textAbstract:
Cryptorchidism is a serious problem, which affects 2–5% of the male population. Failure of the testes to descend into the scrotal region impairs germ cell development and is associated with a greater incidence of testicular cancer. The relaxin-like factor (RLF or insulin-like-3) has been shown to be critically important for the timely descent of the testicles in mice. We have discovered that the signal initiation site of the RLF can be eliminated without measurable effects on hormone binding to its receptor and that the resulting RLF derivative is a competitive inhibitor of RLF called RLFi. RLFi administered to pregnant rats causes dose-dependent gonadal retention in the offspring. The ability to control the severity of the syndrome by altering the concentration of RLFi and the timing of administration enables us to study in detail the structural changes that are associated with the action of RLF during critical stages of development. Targeted inhibition of the physiological migration pattern of testicles by RLFi lets one dissect the physiological process such as to find a window for clinical application of RLF and to search for ancillary factors that might play a role during normal development.
13
Andrade, Bruna Mateus Rocha de, Susana Pimentel Pinto Giannini, André de Campos Duprat, and Léslie Piccolotto Ferreira. "Relação entre a presença de sinais videolaringoscópicos sugestivos de refluxo laringofaríngeo e distúrbio de voz em professoras." CoDAS 28, no. 3 (July 7, 2016): 302–10. http://dx.doi.org/10.1590/2317-1782/20162015122.
Full textAbstract:
RESUMO Objetivo Analisar a relação entre a presença de sinais videolaringoscópicos sugestivos de refluxo laringofaríngeo (RLF) e distúrbio de voz (DV) em professoras. Métodos Pesquisa de natureza transversal, com amostra por conveniência que teve, como critérios de inclusão, ter mais de 18 anos, ser professor do sexo feminino, procurar atendimento com queixa de DV e/ou de RLF. Os fatores de exclusão foram: ser fumante e apresentar alterações respiratórias. Todos os sujeitos preencheram os seguintes instrumentos: Condição de Produção Vocal – Professor (CPV-P), inclusive o Índice de Triagem para Distúrbio de Voz (ITDV), e o Índice de Desvantagem Vocal (IDV). Fez-se coleta de amostra de fala para avaliação perceptivo-auditiva da voz e todas foram submetidas à avaliação otorrinolaringológica. Resultados Foram avaliadas 121 professoras, com média de idade de 43 anos e de 7,8 horas-aula por dia. Somente 24% das professoras não apresentaram lesões em pregas vocais e 42,1% apresentaram sinais videolaringoscópicos sugestivos de RLF. No grupo de professoras com presença de sinais de RLF, os sintomas do ITDV mais relatados foram garganta seca, rouquidão, pigarro, e a média do IDV foi de 17,9 pontos. Não houve associação entre distúrbio de voz e presença de sinais videolaringoscópicos sugestivos de RLF. Na análise de regressão logística binária múltipla, os fatores independentes para o RLF foram idade e escore (tercil: 13-20) do IDV. Conclusão Não houve associação entre o DV e o RLF e sim entre idade e escore IDV.
14
Broxmeyer, HE, L. Lu, DC Bicknell, DE Williams, S. Cooper, S. Levi, J. Salfeld, and P. Arosio. "The influence of purified recombinant human heavy-subunit and light- subunit ferritins on colony formation in vitro by granulocyte- macrophage and erythroid progenitor cells." Blood 68, no. 6 (December 1, 1986): 1257–63. http://dx.doi.org/10.1182/blood.v68.6.1257.1257.
Full textAbstract:
Abstract Purified recombinant human heavy subunit (rHF, acidic) and recombinant human light subunit (rLF, basic) ferritins were assessed for their effects in vitro on colony formation by normal human granulocyte- macrophage (CFU-GM) and erythroid (BFU-E) progenitor cells. The purity of the samples was confirmed by electrophoresis in both nondenaturing and denaturing conditions and silver staining. Concentrations of 10(-8) to 10(-10) mol/L rHF caused an approximately 40% significant decrease in colony formation. Some significant activity was detected at 10(-11) mol/L, and activity was lost at 10(-12) mol/L. In contrast, rLF had no significant activity at 10(-8) to 10(-16) mol/L. rHF was significantly active against mouse bone marrow CFU-GM to concentrations as low as 10(- 8) to 10(-9) mol/L. The inhibitory activity of rHF was inactivated with three different monoclonal antibodies recognizing the heavy subunit of ferritin, but not with two monoclonal antibodies recognizing the light subunit of ferritin. The inhibitory activity of rHF was similar in the absence or presence of serum, monocytes, and T lymphocytes. We and others have shown an association of a glycosylated natural acidic isoferritin (AIF) with inhibitory activity, but since the rHF was expressed in Escherichia coli and did not bind to concanavalin A, glycosylation of AIF is not an absolute prerequisite for this activity. These results demonstrate that rHF has suppressive activity in vitro and substantiate our original observations using purified natural acidic isoferritins.
15
Broxmeyer, HE, L. Lu, DC Bicknell, DE Williams, S. Cooper, S. Levi, J. Salfeld, and P. Arosio. "The influence of purified recombinant human heavy-subunit and light- subunit ferritins on colony formation in vitro by granulocyte- macrophage and erythroid progenitor cells." Blood 68, no. 6 (December 1, 1986): 1257–63. http://dx.doi.org/10.1182/blood.v68.6.1257.bloodjournal6861257.
Full textAbstract:
Purified recombinant human heavy subunit (rHF, acidic) and recombinant human light subunit (rLF, basic) ferritins were assessed for their effects in vitro on colony formation by normal human granulocyte- macrophage (CFU-GM) and erythroid (BFU-E) progenitor cells. The purity of the samples was confirmed by electrophoresis in both nondenaturing and denaturing conditions and silver staining. Concentrations of 10(-8) to 10(-10) mol/L rHF caused an approximately 40% significant decrease in colony formation. Some significant activity was detected at 10(-11) mol/L, and activity was lost at 10(-12) mol/L. In contrast, rLF had no significant activity at 10(-8) to 10(-16) mol/L. rHF was significantly active against mouse bone marrow CFU-GM to concentrations as low as 10(- 8) to 10(-9) mol/L. The inhibitory activity of rHF was inactivated with three different monoclonal antibodies recognizing the heavy subunit of ferritin, but not with two monoclonal antibodies recognizing the light subunit of ferritin. The inhibitory activity of rHF was similar in the absence or presence of serum, monocytes, and T lymphocytes. We and others have shown an association of a glycosylated natural acidic isoferritin (AIF) with inhibitory activity, but since the rHF was expressed in Escherichia coli and did not bind to concanavalin A, glycosylation of AIF is not an absolute prerequisite for this activity. These results demonstrate that rHF has suppressive activity in vitro and substantiate our original observations using purified natural acidic isoferritins.
16
Eckley, Claudia Alessandra, Lilia da Silva Rios, and Luiz Vicente Rizzo. "Estudo comparativo da concentração salivar do fator de crescimento epidérmico em indivíduos com laringite crônica por doença do refluxo gastroesofágica antes e após o tratamento: resultados preliminares." Revista Brasileira de Otorrinolaringologia 73, no. 2 (April 2007): 156–60. http://dx.doi.org/10.1590/s0034-72992007000200003.
Full textAbstract:
Os mecanismos fisiopatológicos do refluxo laringofaríngeo (RLF) são pouco conhecidos. O Fator de Crescimento Epidérmico (EGF) é a proteína de produção salivar com maior ação na regeneração do epitélio da orofaringe e tubo digestivo alto, tendo sido demonstradas deficiências em sua concentração salivar em indivíduos com RLF. OBJETIVO: Comparar a concentração salivar de EGF em um mesmo indivíduo com RLF antes e após o tratamento. MATERIAL E MÉTODO: Neste estudo prospectivo doze indivíduos com DRGE e RLF de moderada intensidade tiveram sua saliva espontânea coletada antes e após o tratamento e controle da doença. A concentração salivar de EGF foi estabelecida através de exame de ELISA (Quantikine ®). RESULTADOS: Onze pacientes eram do sexo feminino e um do sexo masculino, com idade média de 49 anos. A concentração salivar de EGF pré-tratamento foi de 2.867,6pg/mL e a pós-tratamento foi 1.588,5pg/mL, sendo esta diferença estatisticamente significante (p=0,015). DISCUSSÃO E CONCLUSÕES: Apesar de a concentração salivar de EGF ser maior nos indivíduos antes do tratamento, esta não consegue alcançar àquela de uma população normal (estabelecida previamente), o que sugere uma deficiência primária deste importante fator de defesa em indivíduos com RLF.
17
Utomo, Ary Sulistyo. "ALGORITMA FLOODFILL UNTUK MENENTUKAN TITIK KOORDINAT MAZE MAPPING PADA ROBOT LINEFOLLOWER." Simetris : Jurnal Teknik Mesin, Elektro dan Ilmu Komputer 7, no. 1 (April 1, 2016): 227. http://dx.doi.org/10.24176/simet.v7i1.508.
Full textAbstract:
Robot line follower (RLF) adalah robot yang dapat berjalan mengikuti suatu maze yang berupa garis secara otomatis. RLF dapat digunakan untuk aplikasi pengiriman barang dari suatu tempat awal ke tempat tujuan dengan cepat, tepat dan akurat. Untuk menyelesaikan permasalahan tersebut dibutuhkan suatu algoritma yang digunakan untuk mengetahui posisi koordinat robot sehingga robot dapat diketahui pergerakannya. Pergerakan robot berawal dari start dan mecapai titik finish yang telah di tentukan. Pada penelitian ini menggunakan algoritma floodfill untuk mengetahui posisi koordinat RLF. Pengujian dilakukan dengan cara menjalankan RLF dari titik start menuju ke titik finish. Area yang digunakan berukuran 200 x 200 cm mempunyai tebal garis lintasan 2 cm dengan jarak terdekat pada setiap simpangnya adalah 40 cm Warna garis adalah putih dan background berwarna hitam. Hasil penelitian menunjukkan bahwa kestabilan RLF menyusuri garis lintasan dicapai pada nilai pengaturan PID Kp=30, Ki=8 dan KD=100. Hasil dari koordinat pada saat robot bergerak di simpan pada memori eeprom dari mikrokontroller dan di tampilkan pada LCD 2x16. Koordinat start dimulai dengan (0,0) dengan finish (2,2) pada maze yang telah ditentukan. Kata kunci: Robot line follower, floodfill, maze mapping, PID.
18
Sekido, Y., T. Takahashi, T. P. Mäkelä, Y. Obata, R. Ueda, T. Hida, K. Hibi, K. Shimokata, K. Alitalo, and T. Takahashi. "Complex intrachromosomal rearrangement in the process of amplification of the L-myc gene in small-cell lung cancer." Molecular and Cellular Biology 12, no. 4 (April 1992): 1747–54. http://dx.doi.org/10.1128/mcb.12.4.1747.
Full textAbstract:
The L-myc gene was first isolated from a human small-cell lung cancer (SCLC) cell line on the basis of its amplification and sequence similarity to c-myc and N-myc. A new mechanism of L-myc activation which results from the production of rlf-L-myc fusion protein was recently reported. On the basis of our earlier observation of a rearrangement involving amplified L-myc in an SCLC cell line, ACC-LC-49, we decided to investigate this rearrangement in detail along with the structure of L-myc amplification units in five additional SCLC cell lines. We report here the identification of a novel genomic region, termed jal, which is distinct from rlf and is juxtaposed to and amplified with L-myc during the process of DNA amplification of the region encompassing L-myc. Long-range analysis using pulsed-field gel electrophoresis revealed that the amplified L-myc locus is involved in highly complex intrachromosomal rearrangements with jal and/or rlf. Our results also suggest that the simultaneous presence of rearrangements both in rlf intron 1 and in regions immediately upstream of L-myc may be necessary for the expression of rlf-L-myc chimeric transcripts.
19
Sekido, Y., T. Takahashi, T. P. Mäkelä, Y. Obata, R. Ueda, T. Hida, K. Hibi, K. Shimokata, K. Alitalo, and T. Takahashi. "Complex intrachromosomal rearrangement in the process of amplification of the L-myc gene in small-cell lung cancer." Molecular and Cellular Biology 12, no. 4 (April 1992): 1747–54. http://dx.doi.org/10.1128/mcb.12.4.1747-1754.1992.
Full textAbstract:
The L-myc gene was first isolated from a human small-cell lung cancer (SCLC) cell line on the basis of its amplification and sequence similarity to c-myc and N-myc. A new mechanism of L-myc activation which results from the production of rlf-L-myc fusion protein was recently reported. On the basis of our earlier observation of a rearrangement involving amplified L-myc in an SCLC cell line, ACC-LC-49, we decided to investigate this rearrangement in detail along with the structure of L-myc amplification units in five additional SCLC cell lines. We report here the identification of a novel genomic region, termed jal, which is distinct from rlf and is juxtaposed to and amplified with L-myc during the process of DNA amplification of the region encompassing L-myc. Long-range analysis using pulsed-field gel electrophoresis revealed that the amplified L-myc locus is involved in highly complex intrachromosomal rearrangements with jal and/or rlf. Our results also suggest that the simultaneous presence of rearrangements both in rlf intron 1 and in regions immediately upstream of L-myc may be necessary for the expression of rlf-L-myc chimeric transcripts.
20
Yanikkaya, Halit, and Abdullah Altum. "Does capital drain reduce total factor productivity growth in developing countries?" Argumenta Oeconomica 2020, no. 2 (2020): 53–75. http://dx.doi.org/10.15611/aoe.2020.2.03.
Full textAbstract:
This study investigates the effects of foreign direct investment (FDI) and royalties and licence fees (RLF) on total factor productivity (TFP) growth of about 90 countries for the period 2003-2011 for both inward and outward variables. The estimates for the full sample indicate that while inward FDI stocks have no significant impact, outward FDI stocks reduce TFP growth. While none of the RLF measures have any significant effects, imports and exports have significantly positive effects on TFP growth for the full sample. Outward FDI stocks and RLF payments are estimated to have negative effects on TFP growth for developing nations. Moreover, both RLF receipts and payments are found to have a positive effect on TFP growth in developed nations. To stimulate TFP growth further, developing nations should improve their domestic business environments and find ways to keep investments at home
21
Büllesbach, Erika E., and Christian Schwabe. "Tryptophan B27 in the Relaxin-like Factor (RLF) Is Crucial for RLF Receptor-Binding†." Biochemistry 38, no. 10 (March 1999): 3073–78. http://dx.doi.org/10.1021/bi982687u.
Full text
22
Karunanithi, Sheelarani, Tingting Xiong, Maeran Uhm, Dara Leto, Jingxia Sun, Xiao-Wei Chen, and Alan R. Saltiel. "A Rab10:RalA G protein cascade regulates insulin-stimulated glucose uptake in adipocytes." Molecular Biology of the Cell 25, no. 19 (October 2014): 3059–69. http://dx.doi.org/10.1091/mbc.e14-06-1060.
Full textAbstract:
Insulin-stimulated glucose uptake in fat and muscle is mediated by the major facilitative glucose transporter Glut4. Insulin controls the trafficking of Glut4 to the plasma membrane via regulation of a series of small G proteins, including RalA and Rab10. We demonstrate here that Rab10 is a bona fide target of the GTPase-activating protein AS160, which is inhibited after phosphorylation by the protein kinase Akt. Once activated, Rab10 can increase the GTP binding of RalA by recruiting the Ral guanyl nucleotide exchange factor, Rlf/Rgl2. Rab10 and RalA reside in the same pool of Glut4-storage vesicles in untreated cells, and, together with Rlf, they ensure maximal glucose transport. Overexpression of membrane-tethered Rlf compensates for the loss of Rab10 in Glut4 translocation, suggesting that Rab10 recruits Rlf to membrane compartments for RalA activation and that RalA is downstream of Rab10. Together these studies identify a new G protein cascade in the regulation of insulin-stimulated Glut4 trafficking and glucose uptake.
23
Malefahlo, Eliab D., Matt J. Jarvis, Mario G. Santos, Sarah V. White, Nathan J. Adams, and Rebecca A. A. Bowler. "A deep radio view of the evolution of the cosmic star formation rate density from a stellar-mass-selected sample in VLA-COSMOS." Monthly Notices of the Royal Astronomical Society 509, no. 3 (November 1, 2021): 4291–307. http://dx.doi.org/10.1093/mnras/stab3242.
Full textAbstract:
ABSTRACT We present the 1.4 GHz radio luminosity functions (RLFs) of galaxies in the Cosmic Evolution Survey (COSMOS) field, measured above and below the 5σ detection threshold, using a Bayesian model-fitting technique. The radio flux densities from Very Large Array (VLA)-COSMOS 3-GHz data are extracted at the position of stellar-mass-selected galaxies. We fit a local RLF model, which is a combination of active galactic nuclei and star-forming galaxies (SFGs), in 10 redshift bins with a pure luminosity evolution model. Our RLF exceeds previous determinations at low radio luminosities at z < 1.6 with the same radio data, due to our ability to directly constrain the knee and faint-end slope of the RLF. Beyond z ∼ 2, we find that the SFG part of the RLF exhibits a negative evolution (L* moves to lower luminosities) due to the decrease in low stellar-mass galaxies in our sample at high redshifts. From the RLF for SFGs, we determine the evolution in the cosmic star formation rate density (SFRD), which we find to be consistent with the established behaviour up to z ∼ 1 using far-infrared data, but exceeds that from the previous radio-based work for the reasons highlighted above. Beyond z ∼ 1.5 the cosmic SFRD declines. We note that the relation between radio luminosity and star formation rate is crucial in measuring the cosmic SFRD from radio data at z > 1.5. We investigate the effects of stellar mass on the total RLF by splitting our sample into low (108.5 ≤ M/M⊙ ≤ 1010) and high ($M\gt 10^{10}\, \mathrm{M}_{\odot }$) stellar-mass subsets. We find that the SFRD is dominated by sources in the high stellar masses bin, at all redshifts.
24
Ferro, Elisa, David Magrini, Paolo Guazzi, Thomas H. Fischer, Sara Pistolesi, Rebecca Pogni, Gilbert C. White, and Lorenza Trabalzini. "G-protein binding features and regulation of the RalGDS family member, RGL2." Biochemical Journal 415, no. 1 (September 12, 2008): 145–54. http://dx.doi.org/10.1042/bj20080255.
Full textAbstract:
RGL2 [RalGDS (Ral guanine nucleotide dissociation stimulator)-like 2] is a member of the RalGDS family that we have previously isolated and characterized as a potential effector for Ras and the Ras analogue Rap1b. The protein shares 89% sequence identity with its mouse orthologue Rlf (RalGDS-like factor). In the present study we further characterized the G-protein-binding features of RGL2 and also demonstrated that RGL2 has guanine-nucleotide-exchange activity toward the small GTPase RalA. We found that RGL2/Rlf properties are well conserved between human and mouse species. Both RGL2 and Rlf have a putative PKA (protein kinase A) phosphorylation site at the C-terminal of the domain that regulates the interaction with small GTPases. We demonstrated that RGL2 is phosphorylated by PKA and phosphorylation reduces the ability of RGL2 to bind H-Ras. As RGL2 and Rlf are unique in the RalGDS family in having a PKA site in the Ras-binding domain, the results of the present study indicate that Ras may distinguish between the different RalGDS family members by their phosphorylation by PKA.
25
NK Nababan, Marlince, and Yonata Laia. "Pemanfaatan Metode Recursive Largest First Dalam Penyusunan Shift Kerja Karyawan Pada Rumah Sakit Royal Prima Medan." Jurnal Teknik Komputer 6, no. 1 (January 10, 2020): 130–34. http://dx.doi.org/10.31294/jtk.v6i1.6877.
Full textAbstract:
Penysusunan jadwal karywan merupakan salah satu komponen penting di setiap perusahaan yang memiliki karyawan yang dalam jumlah benyak. Dimana pada Rumah Sakit Umum Roal Prima memiliki jumlah karyawan 600 orang dengan itulah karyawan yang bersangkutan mengalami kendalah dalam penyusunan jadwal karyawan. Berdasarkan data yang di dapatkan dari karyawan yang bersangkutan mengatakan penyususan penjadwalan kawayan dengan jaumlah 600 orang membutuhkan waktu 1 miggu. Pada penelitian menggunakan metode RLF dalam penysusunan penjadwawaln karyawan. Berdasarkan hasil yang di dapatkan dengan metode yang digunakan adalah pengerjaan penyusunan penjadwalan karyawan denga metode RLF lebih baik dari pada pekerjaan sebleummnya. Dimana pada metode yang sebelummnya harus menunggu waktu seminggu dalam pengerjaan penyusuanan penejadwalan karywan, dengan aplikasi memekau metode RLF ini dapat terselesaikan dalam 1 hari.
26
Smith, Nancy R., Stephen Benson, and Carolyn Larabell. "IVEM and SEM images of whole cells cultured on EHS matrix (Matrigel)." Proceedings, annual meeting, Electron Microscopy Society of America 49 (August 1991): 186–87. http://dx.doi.org/10.1017/s042482010008523x.
Full textAbstract:
To investigate the interactions of cells with the extracellular matrix (ECM), rat lung fibroblasts (RLF) were cultured on an ECM extracted from EHS sarcoma to serve as an in vitro morphogenic model system. Surface features of RLF/EHS matrix interactions were viewed using the scanning electron microscope (SEM) and the intermediate voltage electron microscope (IVEM).For SEM, RLF were plated on EHS coated 13 mm glass coverslips at a density of 1 X 104/cm2. Coverslips were coated by spreading 25 μl of chilled EHS matrix (15 mg/ml) on the coverslip and incubating at 37° for 60 min. For IVEM, formvar coated gold grids were submerged in EHS matrix prior to polymerization. Controls consisted of cells grown on glass or gold grids minus ECM.
27
Malefahlo, Eliab, Mario G. Santos, Matt J. Jarvis, Sarah V. White, and Jonathan T. L. Zwart. "The optically selected 1.4-GHz quasar luminosity function below 1 mJy." Monthly Notices of the Royal Astronomical Society 492, no. 4 (January 15, 2020): 5297–312. http://dx.doi.org/10.1093/mnras/staa112.
Full textAbstract:
ABSTRACT We present the radio luminosity function (RLF) of optically selected quasars below 1 mJy, constructed by applying a Bayesian-fitting stacking technique to objects well below the nominal radio flux density limit. We test the technique using simulated data, confirming that we can reconstruct the RLF over three orders of magnitude below the typical 5σ detection threshold. We apply our method to 1.4-GHz flux densities from the Faint Images of the Radio Sky at Twenty-Centimeters (FIRST) survey, extracted at the positions of optical quasars from the Sloan Digital Sky Survey over seven redshift bins up to z = 2.15, and measure the RLF down to two orders of magnitude below the FIRST detection threshold. In the lowest redshift bin (0.2 < z < 0.45), we find that our measured RLF agrees well with deeper data from the literature. The RLF for the radio-loud quasars flattens below $\log _{10}[L_{1.4}/{\rm W\, Hz}^{-1}] \approx 25.5$ and becomes steeper again below $\log _{10}[L_{1.4}/{\rm W\, Hz}^{-1}] \approx 24.8$, where radio-quiet quasars start to emerge. The radio luminosity where radio-quiet quasars emerge coincides with the luminosity where star-forming galaxies are expected to start dominating the radio source counts. This implies that there could be a significant contribution from star formation in the host galaxies, but additional data are required to investigate this further. The higher redshift bins show a similar behaviour to the lowest z bin, implying that the same physical process may be responsible.
28
Syam, Rahmat, Hisyam Ihsan, and A. Asman. "Aplikasi Pewarnaan Graf dengan Algoritma Recursive Largest First pada Penjadwalan Mata Kuliah." Journal of Mathematics, Computations, and Statistics 2, no. 1 (May 12, 2020): 63. http://dx.doi.org/10.35580/jmathcos.v2i1.12461.
Full textAbstract:
Penelitian ini merupakan penelitian terapan yang menggunakan algoritma Recursive Largest First (RLF) pada penjadwalan mata kuliah di salah satu program studi pada salah satu universitas di Makassar. Penjadwalan mata kuliah dilakukan dengan merepresentasikan masalah ke dalam bentuk graf berdasarkan data yang diperoleh dengan menganggap mata kuliah sebagai simpul dan dosen sebagai sisi. Dibentuk matriks ketetanggaan dari graf yang terbentuk, kemudian simpulya diwarnai menggunakan algoritma RLF, sehingga diperoleh jadwal kuliah. Penjadwalan mata kuliah menggunakan pewarnaan simpul graf dengan algoritma RLF digunakan VBA for Microsoft Excel 2016 sebagai alat bantu dalam pewarnaan simpul graf. Diperoleh 102 simpul dengan 25 warna yang menunjukkan bahwa dibutuhkan 25 waktu perkuliahan. Jumlah simpul yang diwarnai dengan warna yang sama 6 simpul artinya dalam setiap slot dibutuhkan maksimal 6 ruangan perkuliahan. Mata kuliah yang terjadwal dijamin bahwa tidak ada jadwal dosen yang bersamaan untuk setiap mata kuliah yang diajarkan.Kata Kunci: Graf, Recursive Largest First, Jadwal Kuliah This research discusses the colorization of graph vertex using algorithm of Recursive Largest First (RLF) in scheduling courses in one of the study programs at one of the universities in Makassar. Representation of problems in scheduling courses was done by making it in the shape of the graph assuming subjects as vertex and the presence of the same lecturers teach different courses as a side. The steps in solving a problem that was mapping the lectures data, representing the lectures data as a graph, forming adjacency matrix, coloring the graph vertex using algorithm of RLF, so that the schedule was retrieved. Scheduling of courses using the colorization of graph vertex with algorithm of RLF as well as the help of VBA for Microsoft Excel 2016 at Faculty of Animal Husbandry obtained 102 vertices with 25 colors indicates that it takes 25 of lecture schedules. The number of vertices that were colored with the same color ≤ 6 vertices meaning in every slot needed 6 lecture rooms in maximum. Scheduled courses are guaranteed that no schedule conflicts for each lecturer of courses taught.Keywords: Graph, Recursive Largest First, Lecture Schedules.
29
Sahyuni, Riza, Bambang Supriyatno, Syahrial MH, and Aria Kekalih. "Efektifitas Pemberian Proton Pump Inhibitor pada Bayi dan Anak dengan Laringomalasia." Sari Pediatri 18, no. 6 (June 16, 2017): 459. http://dx.doi.org/10.14238/sp18.6.2017.459-67.
Full textAbstract:
Latar belakang. Laringomalasia merupakan kondisi kelemahan struktur supraglotis yang menimbulkan gejala stridor. Umumnya, penyakit penyerta laringomalasia adalah refluks laringofaring (RLF). Pemberian omeperazol dapat memperbaiki gejala regurgitasi dan stridor serta memperpendek durasi perjalanan alamiah laringomalasia.Tujuan. Mengetahui efektifitas omeperazol pada bayi dan anak dengan laringomalasia.Metode. Uji pre-post experimental pada 42 subyek yang mendapat omeperazol 2x2 mg/kg/bb selama 3 bulanHasil. Pemberian omeperazol pada laringomalasia dengan gejala berat, 58,8% mengalami perbaikan. Pemberian omeperazol pada laringomalasia dengan RLF positif, 58,3% mengalami perbaikan. Tampak perbaikan bermakna secara statistik pada perbandingan nilai awal dan akhir reflux finding score (RFS) dan laringomalacia score symptom (LSS)Kesimpulan. Prevalensi RLF positif berdasar RFS menurut Belafsky adalah 24,6% dan berdasar LSS dengan gejala berat adalah 44,6%. Efektifitas pemberian omeperazol selama 3 bulan terbukti efektif pada perbaikan nilai LSS, RFS dan status gizi.
30
Ronquim, Carlos Cesar, and Adhemar Ronquim Filho. "Limitação ao uso Agrícola da Propriedade Rural pela Instituição de Reserva Legal Florestal." Revista Brasileira Multidisciplinar 12, no. 2 (January 16, 2009): 133. http://dx.doi.org/10.25061/2527-2675/rebram/2009.v12i2.163.
Full textAbstract:
O presente texto aborda as limitações de proteção especial dos recursos naturais nas propriedades agrícolas representadas pela Reserva Legal Florestal (RLF) a partir de sua origem, até a forma pela qual é tutelada atualmente. São estabelecidas as suas relações e diferenciações. Formas para as recomposições e utilização da RLF são abordadas. Por fim, são ressaltados os argumentos dos setores ambientalistas e ruralistas para o aperfeiçoamento do instituto e sua aplicabilidade, apresentando-se formas de ser posto em prática o almejado desenvolvimento sustentável.
31
Adegbindin, Mourchid, Alain Hertz, and Martine Bellaïche. "A new efficient RLF-like algorithm for the vertex coloring problem." Yugoslav Journal of Operations Research 26, no. 4 (2016): 441–56. http://dx.doi.org/10.2298/yjor151102003a.
Full textAbstract:
The Recursive Largest First (RLF) algorithm is one of the most popular greedy heuristics for the vertex coloring problem. It sequentially builds color classes on the basis of greedy choices. In particular, the first vertex placed in a color class C is one with a maximum number of uncolored neighbors, and the next vertices placed in C are chosen so that they have as many uncolored neighbors which cannot be placed in C. These greedy choices can have a significant impact on the performance of the algorithm, which explains why we propose alternative selection rules. Computational experiments on 63 difficult DIMACS instances show that the resulting new RLF-like algorithm, when compared with the standard RLF, allows to obtain a reduction of more than 50% of the gap between the number of colors used and the best known upper bound on the chromatic number. The new greedy algorithm even competes with basic metaheuristics for the vertex coloring problem.
32
Song, Jinmiao, Shengwei Tian, Long Yu, Qimeng Yang, Qiguo Dai, Yuanxu Wang, Weidong Wu, and Xiaodong Duan. "RLF-LPI: An ensemble learning framework using sequence information for predicting lncRNA-protein interaction based on AE-ResLSTM and fuzzy decision." Mathematical Biosciences and Engineering 19, no. 5 (2022): 4749–64. http://dx.doi.org/10.3934/mbe.2022222.
Full textAbstract:
<abstract><p>Long non-coding RNAs (lncRNAs) play a regulatory role in many biological cells, and the recognition of lncRNA-protein interactions is helpful to reveal the functional mechanism of lncRNAs. Identification of lncRNA-protein interaction by biological techniques is costly and time-consuming. Here, an ensemble learning framework, RLF-LPI is proposed, to predict lncRNA-protein interactions. The RLF-LPI of the residual LSTM autoencoder module with fusion attention mechanism can extract the potential representation of features and capture the dependencies between sequences and structures by k-mer method. Finally, the relationship between lncRNA and protein is learned through the method of fuzzy decision. The experimental results show that the ACC of RLF-LPI is 0.912 on ATH948 dataset and 0.921 on ZEA22133 dataset. Thus, it is demonstrated that our proposed method performed better in predicting lncRNA-protein interaction than other methods.</p></abstract>
33
Cielo, Carla Aparecida, Dayane Domeneghini Didoné, Enma Mariángel Ortiz Torres, and Joziane Padilha de Moraes Lima. "Refluxo laringofaríngeo e bulimia nervosa: alterações vocais e larínegas." Revista CEFAC 13, no. 2 (December 3, 2010): 352–61. http://dx.doi.org/10.1590/s1516-18462010005000130.
Full textAbstract:
TEMA: Características da bulimia nervosa (BN) e do Refluxo Laringofaríngeo (RLF) e alterações vocais e laríngeas que podem decorrer da exposição da laringe ao ácido gástrico. OBJETIVO: Descrever, por meio de revisão de literatura, as características do RLF e da BN, relacionando-os com as alterações vocais e laríngeas que podem se originar da exposição da laringe ao ácido gástrico que ocorre em ambas as condições. CONCLUSÃO: A BN se caracteriza por um transtorno alimentar de causa principalmente psicológica em que ocorre ingestão compulsiva de grande quantidade de alimentos, seguida de episódios de vômitos auto-induzidos com a finalidade de evitar o ganho de peso. O RLF consiste em uma variação clínica da DRGE, na qual o fluxo retrógrado do alimento e do ácido gástrico entra em contato com a laringe e suas estruturas. Em comum, a BN e o RLF apresentam este mecanismo de regurgitação que pode atingir a laringe. Dentre os sinais e sintomas laríngeos e vocais decorrentes da regurgitação do ácido gástrico sobre a laringe estão: globus faríngeo, disfonia, rouquidão, tosse seca, disfagia, halitose, lesões inflamatórias nas pregas vocais, laringite, amigdalite, edema e eritema glótico posterior e ou de aritenóides, da região retrocricóidea e interaritenóidea, estenose subglótica, irregularidades na mucosa das pregas vocais, hipertrofia de mucosa laríngea, leucoplasia, carcinoma, laringoespasmos, granulomas, úlcera de contato, nódulos vocais, pólipos vocais, edema difuso, granuloma, fixação uni ou bilateral de aritenóides, edema de Reinke, laringomalácia, estridor, e odinofagia.
34
Eckley, Claudia Alessandra, and Henrique Olival Costa. "Estudo comparativo do pH e do volume salivar em indivíduos com laringofaringite crônica por doença do refluxo gastroesofágica antes e após o tratamento." Revista Brasileira de Otorrinolaringologia 72, no. 1 (February 2006): 55–60. http://dx.doi.org/10.1590/s0034-72992006000100010.
Full textAbstract:
INTRODUÇÃO: A Doença do Refluxo Gastroesofágico (DRGE) é a doença digestiva mais prevalente da atualidade e, recentemente, tem sido implicada em uma gama de alterações do seguimento laringofaríngeo (RLF). No entanto, pouco se sabe dos mecanismos fisiopatológicos destas manifestações supraesofágicas da DRGE. Os achados clínicos contraditórios e recentes pesquisas sugerem haver deficiências na capacidade de defesa deste seguimento. Uma das principais responsáveis pela homeostase da mucosa oral e do trato digestivo é a saliva com seu conteúdo orgânico e inorgânico. Tanto alterações do pH quanto do volume salivar já foram correlacionados com os sintomas e sinais sugestivos da DRGE e RLF. Estudo recente de nossa autoria demonstra diminuição estatisticamente significante do pH salivar de indivíduos com RLF quando comparado a controles sem a doença. Outro estudo constatou correlação entre a redução do volume X pH da saliva em indivíduos com DRGE, estando esta redução diretamente relacionada aos níveis de pH esofágico constatados durante pH-metria esofágica de 24 horas. OBJETIVOS: Avaliar como se comportam o pH e volume da saliva em um mesmo indivíduo com DRGE e RLF antes e após o tratamento clínico. MATERIAL E MÉTODO: Vinte e três pacientes com RLF tiveram o pH e volume da saliva total testados antes e após receberem tratamento com droga bloqueadora de bomba de prótons durante 12 semanas. RESULTADOS: Houve uma diferença estatisticamente significante (p<0,001) entre o pH da saliva antes e após o tratamento, estando este maior após o controle clínico da doença. O volume de saliva no paciente tratado foi significativamente maior do que no paciente pré-tratamento (p=0.009). DISCUSSÃO: Os achados sugerem que o pH salivar é influenciado pela presença de refluxo gastroduodenal à região laringofaríngea. Caso estudos futuros com populações maiores realmente comprovem esta correlação, poderemos cogitar a possibilidade de usar a mensuração do pH salivar, que é feita de forma rápida e não invasiva, como um meio de diagnosticar e avaliar o comportamento e controle do Refluxo Laringofaríngeo.
35
Ivell, R. "Biology of the relaxin-like factor (RLF)." Reviews of Reproduction 2, no. 3 (September 1, 1997): 133–38. http://dx.doi.org/10.1530/revreprod/2.3.133.
Full text
36
Ivell, R. "Biology of the relaxin-like factor (RLF)." Reviews of Reproduction 2, no. 3 (September 1, 1997): 133–38. http://dx.doi.org/10.1530/ror.0.0020133.
Full text
37
Rusinek-Abarca, Katarzyna, and Marek Sikora. "The Dependence of the Fraction of Radio Luminous Quasars on Redshift and its Theoretical Implications." Astrophysical Journal 922, no. 2 (November 30, 2021): 202. http://dx.doi.org/10.3847/1538-4357/ac2429.
Full textAbstract:
Abstract While radio emission in quasars can be contributed to by a variety of processes (involving star-forming regions, accretion disk coronas and winds, and jets), the powering of the radio loudest quasars must involve very strong jets, presumably launched by the Blandford–Znajek mechanism incorporating the magnetically arrested disk (MAD) scenario. We focus on the latter and investigate the dependence of their fraction on redshift. We also examine the dependence of the radio-loud fraction (RLF) on BH mass (M BH) and Eddington ratio (λ Edd), while excluding the redshift bias by narrowing its range. In both of these investigations, we remove the bias associated with: (1) the diversity of source selection by constructing two well-defined, homogeneous samples of quasars (first within 0.7 ≤ z ≤ 1.9, second within 0.5 ≤ z ≤ 0.7); and (2) a strong drop in the RLF of quasars at smaller BH masses by choosing those with BH masses larger than 108.5 M ⊙. We confirm some of the previous results showing the increase in the fraction of radio-loud quasars with cosmic time and that this trend can be even steeper if we account for the bias introduced by the dependence of the RLF on BH mass, whereas the bias introduced by the dependence of the RLF on Eddington ratio is shown to be negligible. Assuming that quasar activities are triggered by galaxy mergers, we argue that such an increase can result from the slower drop with cosmic time of mixed mergers than of wet mergers.
38
Lanigan, Thomas M., Albert Liu, Yang Z. Huang, Lin Mei, Ben Margolis, and Kun-Liang Guan. "Human homologue of Drosophila CNK interacts with Ras effector proteins Raf and Rlf 1." FASEB Journal 17, no. 14 (November 2003): 2048–60. http://dx.doi.org/10.1096/fj.02-1096com.
Full text
39
Peyssonnaux, Carole, Sylvain Provot, Marie Paule Felder-Schmittbuhl, Georges Calothy, and Alain Eychène. "Induction of Postmitotic Neuroretina Cell Proliferation by Distinct Ras Downstream Signaling Pathways." Molecular and Cellular Biology 20, no. 19 (October 1, 2000): 7068–79. http://dx.doi.org/10.1128/mcb.20.19.7068-7079.2000.
Full textAbstract:
ABSTRACT Ras-induced cell transformation is mediated through distinct downstream signaling pathways, including Raf, Ral-GEFs-, and phosphatidylinositol 3-kinase (PI 3-kinase)-dependent pathways. In some cell types, strong activation of the Ras–Raf–MEK–extracellular signal-regulated kinase (ERK) cascade leads to cell cycle arrest rather than cell division. We previously reported that constitutive activation of this pathway induces sustained proliferation of primary cultures of postmitotic chicken neuroretina (NR) cells. We used this model system to investigate the respective contributions of Ras downstream signaling pathways in Ras-induced cell proliferation. Three RasV12 mutants (S35, G37, and C40) which differ by their ability to bind to Ras effectors (Raf, Ral-GEFs, and the p110 subunit of PI 3-kinase, respectively) were able to induce sustained NR cell proliferation, although none of these mutants was reported to transform NIH 3T3 cells. Furthermore, they all repressed the promoter of QR1, a neuroretina growth arrest-specific gene. Overexpression of B-Raf or activated versions of Ras effectors Rlf-CAAX and p110-CAAX also induced NR cell division. The mitogenic effect of the RasC40–PI 3-kinase pathway appears to involve Rac and RhoA GTPases but not the antiapoptotic Akt (protein kinase B) signaling. Division induced by RasG37-Rlf appears to be independent of Ral GTPase activation and presumably requires an unidentified mechanism. Activation of either Ras downstream pathway resulted in ERK activation, and coexpression of a dominant negative MEK mutant or mKsr-1 kinase domain strongly inhibited proliferation induced by the three Ras mutants or by their effectors. Similar effects were observed with dominant negative mutants of Rac and Rho. Thus, both the Raf-MEK-ERK and Rac-Rho pathways are absolutely required for Ras-induced NR cell division. Activation of these two pathways by the three distinct Ras downstream effectors possibly relies on an autocrine or paracrine loop, implicating endogenous Ras, since the mitogenic effect of each Ras effector mutant was inhibited by RasN17.
40
Ceraj, L., V. Smolčić, I. Delvecchio, A. Butler, K. Tisanić, J. Delhaize, C. Horellou, et al. "The XXL Survey." Astronomy & Astrophysics 642 (October 2020): A125. http://dx.doi.org/10.1051/0004-6361/201936776.
Full textAbstract:
We studied a sample of 274 radio and X-ray selected quasars (XQSOs) detected in the COSMOS and XXL-S radio surveys at 3 GHz and 2.1 GHz, respectively. This sample was identified by adopting a conservative threshold in X-ray luminosity, LX [2−10 keV] ≥ 1044 erg s−1, selecting only the most powerful quasars. A number of previous studies on the origin of radio emission in type-1 quasars have focused on the radio loudness distributions, some claiming to have found evidence for bimodality, pointing toward the existence of two physically different mechanisms for the radio emission. Using available multiwavelength data, we examined various criteria for the selection of radio-loud (RL) and radio-quiet (RQ) XQSOs and found that the number of RL/RQ XQSOs changes significantly depending on the chosen criterion. This discrepancy arises due to the different criteria tracing different physical processes and due to the fact that our sample was selected from flux-limited radio and X-ray surveys. Another approach to study the origin of radio emission in XQSOs is via their radio luminosity functions (RLF). We constructed the XQSO 1.4 GHz RLFs in six redshift bins at 0.5 ≤ z ≤ 3.75. The lower-1.4 GHz luminosity end shows a higher normalization than expected only from AGN contribution in all studied redshift bins. We found that the so-called “bump” is mostly dominated by emission due to star-forming processes within the host galaxies of XQSOs. As expected, AGN-related radio emission is the dominant contribution at the higher-luminosity end of RLF. To study the evolution of the XQSO RLF, we used a combination of analytic forms from the literature to constrain the “bump” due to star formation and the higher-luminosity AGN part of the RLF. We defined two 1.4 GHz luminosity thresholds, Lth, SF and Lth, AGN, below and above which more than 80% of sources contributing to the RLF are dominated by star formation and AGN-related activity, respectively. The two thresholds evolve with redshift, which is most likely driven by the strong evolution of star formation rates of the XQSO host galaxies. We found that both the lower and higher luminosity ends evolve significantly in density, while their luminosity evolution parameters are consistent with being constant. We found that the lower-luminosity end evolves both in density and luminosity, while the higher-luminosity end evolves significantly only in density. Our results expose the dichotomy of the origin of radio emission: while the higher-luminosity end of the XQSO RLF is dominated by AGN activity, the lower-luminosity end is dominated by the star formation-related processes.
41
Buulolo, Finus, and R. Mahdalena Simanjorang. "Application Employee Shift Scheduling Algorithm Recursive Largest First (RLF) at PT. Invilon Sagita Medan." Journal Of Computer Networks, Architecture and High Performance Computing 2, no. 1 (January 1, 2020): 83–87. http://dx.doi.org/10.47709/cnapc.v2i1.362.
Full textAbstract:
Scheduling is one of the important things in any company such as PT. Invilon sagita currently have employees reaches hundreds of people so that employees who handle scheduling of employees experience difficulty in because of the number of employees is too much. Constraints in the natural is a very long time so it is necessary a method in solving the problem. In this study using Lagert Recusiv First (RLF) in the preparation of Karawan scheduling with visual studio 2010. program based on those already in the test results with the number of employees by 5 people RLF method can resolve employee scheduler quickly.
42
Cao, Zhe, Shunzhao Sui, Qian Yang, and Zhanao Deng. "Inheritance of Rugose Leaf in Caladium and Genetic Relationships with Leaf Shape, Main Vein Color, and Leaf Spotting." Journal of the American Society for Horticultural Science 141, no. 5 (September 2016): 527–34. http://dx.doi.org/10.21273/jashs03854-16.
Full textAbstract:
A number of caladium cultivars (Caladium ×hortulanum), including Miss Muffet and Gingerland, produce rugose leaves. The rugosity on these leaves is an intriguing characteristic, often resulting in an increased ornamental value. This study was conducted to understand the mode of inheritance of this trait and to determine its genetic relationship with other foliar characteristics including leaf shape, main vein color, and leaf spotting in caladiums. Sixteen caladium cultivars/breeding lines were crossed and 20 populations were produced; progeny of these populations were phenotyped for rugose leaf as well as leaf shape, main vein color, and leaf spotting. Results showed that a single locus with two alleles controlled the presence or absence of rugose leaves in these populations. The locus was designated as RLF, with the dominant RLF allele for rugose leaves and the recessive allele rlf for nonrugose (flat) leaves. Rugose cultivars Miss Muffet and Gingerland and breeding line UF-317 possessed the heterozygous genotype RLFrlf. Rugose leaf was inherited independently from leaf shape, but linked with the green main vein allele (Vg) at the V locus and the leaf spotting allele (S) at the S locus. Three-point analysis of the segregation of the three linked traits in reciprocal crosses between ‘Miss Muffet’ and nonrugose ‘Candidum’ indicated a genetic linkage map with the gene order of S locus locating between the V and the RLF loci. The information obtained from this study will be useful for developing breeding strategies for producing new caladium cultivars with or without rugose leaves, and can facilitate the understanding of the mode of inheritance for rugose leaves in other aroids and other plants.
43
Yao, Qiong, Dan Song, Xiang Xu, and Kun Zou. "A Novel Finger Vein Recognition Method Based on Aggregation of Radon-Like Features." Sensors 21, no. 5 (March 8, 2021): 1885. http://dx.doi.org/10.3390/s21051885.
Full textAbstract:
Finger vein (FV) biometrics is one of the most promising individual recognition traits, which has the capabilities of uniqueness, anti-forgery, and bio-assay, etc. However, due to the restricts of imaging environments, the acquired FV images are easily degraded to low-contrast, blur, as well as serious noise disturbance. Therefore, how to extract more efficient and robust features from these low-quality FV images, remains to be addressed. In this paper, a novel feature extraction method of FV images is presented, which combines curvature and radon-like features (RLF). First, an enhanced vein pattern image is obtained by calculating the mean curvature of each pixel in the original FV image. Then, a specific implementation of RLF is developed and performed on the previously obtained vein pattern image, which can effectively aggregate the dispersed spatial information around the vein structures, thus highlight vein patterns and suppress spurious non-boundary responses and noises. Finally, a smoother vein structure image is obtained for subsequent matching and verification. Compared with the existing curvature-based recognition methods, the proposed method can not only preserve the inherent vein patterns, but also eliminate most of the pseudo vein information, so as to restore more smoothing and genuine vein structure information. In order to assess the performance of our proposed RLF-based method, we conducted comprehensive experiments on three public FV databases and a self-built FV database (which contains 37,080 samples that derived from 1030 individuals). The experimental results denoted that RLF-based feature extraction method can obtain more complete and continuous vein patterns, as well as better recognition accuracy.
44
Liang, Gui-Chou, Yen-Chieh Ouyang, and Shu-Mei Dai. "Detection and Classification of Rice Infestation with Rice Leaf Folder (Cnaphalocrocis medinalis) Using Hyperspectral Imaging Techniques." Remote Sensing 13, no. 22 (November 15, 2021): 4587. http://dx.doi.org/10.3390/rs13224587.
Full textAbstract:
The detection of rice leaf folder (RLF) infestation usually depends on manual monitoring, and early infestations cannot be detected visually. To improve detection accuracy and reduce human error, we use push-broom hyperspectral sensors to scan rice images and use machine learning and deep neural learning methods to detect RLF-infested rice leaves. Different from traditional image processing methods, hyperspectral imaging data analysis is based on pixel-based classification and target recognition. Since the spectral information itself is a feature and can be considered a vector, deep learning neural networks do not need to use convolutional neural networks to extract features. To correctly detect the spectral image of rice leaves infested by RLF, we use the constrained energy minimization (CEM) method to suppress the background noise of the spectral image. A band selection method was utilized to reduce the computational energy consumption of using the full-band process, and six bands were selected as candidate bands. The following method is the band expansion process (BEP) method, which is utilized to expand the vector length to improve the problem of compressed spectral information for band selection. We use CEM and deep neural networks to detect defects in the spectral images of infected rice leaves and compare the performance of each in the full frequency band, frequency band selection, and frequency BEP. A total of 339 hyperspectral images were collected in this study; the results showed that six bands were sufficient for detecting early infestations of RLF, with a detection accuracy of 98% and a Dice similarity coefficient of 0.8, which provides advantages of commercialization of this field.
45
Jiang, Li-Ben, Jia Cheng, Zhan-Fei Zhu, Lin-Quan Ge, Guo-Qing Yang, and Jin-Cai Wu. "Impact of Day Intervals on Sequential Infestations of the Rice Leaffolder Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Pyralidae) and the White-Backed Rice Planthopper Sogatella furcifera (Horváth) on Rice Grain Damage." International Journal of Insect Science 6 (January 2014): IJIS.S13536. http://dx.doi.org/10.4137/ijis.s13536.
Full textAbstract:
The present study illustrates that different day intervals (DIs) between the sequential infestations of two pest species, the rice leaffolder (RLF) Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae) and the white-backed rice planthopper (WBPH) Sogatella furcifera Horváth (Hemiptera: Delphacidae), have a significant impact on the rice yield loss rate (YLR) and on the carbohydrate contents of rice plants. For WBPH release after RLF release (WRARR), the YLR decreased with the increasing DIs, and the YLR at the 24 DI was significantly lower compared to that at the 6 and 12 DIs and had a minimum value for a simultaneous infestation of the two pest species (SITS). In contrast, for RLF release after WBPH release (RRAWR), the YLR at the 24 DI had a maximum value and was significantly higher compared to that at the 6 and 12 DIs and the SITS. These findings indicate that damaged rice plants gradually recover, with an increase in the DI for WRARR. The above results were demonstrated by biochemical tests. Therefore, the sequential infestation of the two pest species and their DIs should be considered for integrated pest management (IPM) and control strategies for rice pests.
46
Данилов, А. А., А. В. Шульга, and В. В. Горелик. "Conservative Treatment of the Rigid Form of Longitudinal Flatfeet in Children." Педиатрия. Восточная Европа, no. 1 (March 17, 2022): 48–58. http://dx.doi.org/10.34883/pi.2022.10.1.012.
Full textAbstract:
Введение. Значительный процент патологии опорно-двигательной системы у детей в настоящее время приходится на патологию стопы – продольное плоскостопие, которое является не только медицинской, но и социальной проблемой. Частота, с какой встречается данная патология, составляет до 58% от общей ортопедической патологии стопы. Традиционные методы лечения и профилактики легкой (І–ІІ) степени тяжести ригидного продольного плоскостопия (РПП), которые известны сегодня (массаж, лечебная физкультура, использование ортопедических изделий, электромиостимуляция), не дают значительного эффекта и стабильного, долговременного результата. Это заставляет искать более эффективные методы консервативного лечения РПП І–ІІ степеней тяжести, которые имеют патогенетическую направленность и физиологическое влияние.Цель. Разработать наиболее эффективный алгоритм комплексного консервативного лечения РПП в зависимости от степени тяжести, нарушений гемодинамики, вариантов деформаций, ригидности стопы.Материалы и методы. Консервативное лечение проводилось 52 пациентам в возрасте 10–18 лет с РПП І–ІІ степени тяжести. В зависимости от клинических проявлений и результатов обследования лечение проводилось в два этапа. На первом использовали этапное гипсование в сочетании с силиконовыми пилотами. На втором этапе применялись биогенные стимуляторы.Результаты. Результаты двухэтапного консервативного лечения РПП свидетельствуют об эффективности использования этапного гипсования в сочетании с применением силиконовых пилотов и биостимуляторов в сравнении с традиционными методиками коррекции стопы.Заключение. Разработаны алгоритмы консервативного лечения РПП в зависимости от степени тяжести, характера деформации, нарушения гемодинамики и тонуса мышц голени и стопы. Introduction. A significant percentage of the musculoskeletal system pathology in children is currently fall on the foot pathology – longitudinal flatfeet, which is not only a medical, but also a social problem. The incidence of this pathology is up to 58% of the total orthopedic foot pathology. Traditional methods of treatment and prevention of mild (I–II severity) rigid longitudinal flatfeet (hereinafter referred to as RLF), which are known today (massage, physiotherapy exercises, use of orthopedic products, electromyostimulation) do not give a significant effect and stable, long-term result. This makes us look for more effective methods of conservative treatment of RLF of the I–II degrees of severity that have a pathogenic orientation and physiological effect.Purpose. Тo develop the most effective algorithm for the complex conservative treatment of RLF, depending on the severity, hemodynamic disorders, deformity options, foot rigidity.Materials and methods. Conservative treatment was carried out in 52 patients aged 10–18 years with RLF of the I–II severity. Depending on the clinical manifestations and examination results, the treatment was carried out in two stages. At the first stage, serial casting was used (in the presence of concomitant deformations – silicone pilots were used).Results. The results of the two-stage conservative treatment of RLF demonstrate the effectiveness of serial casting in combination with the use of silicone pilots and biostimulators in comparison with traditional methods of foot correction.Conclusion. Algorithms for conservative treatment of RLF were developed, depending on the severity, nature of the deformity, hemodynamic disorder and muscle tone of the leg and foot.
47
Trang, Kathy. "Call for Applications: 2017-2018 SPA/RLF Fellows." Anthropology News 58, no. 1 (January 2017): e308-e309. http://dx.doi.org/10.1111/an.321.
Full text
48
Boockfor, F. "Relaxin-like factor (RLF) serum concentrations and gubernaculum RLF receptor display in relation to pre- and neonatal development of rats." Reproduction 122, no. 6 (December 1, 2001): 899–906. http://dx.doi.org/10.1530/reprod/122.6.899.
Full text
49
Asyari, Ade, Deni Amri, Novialdi Novialdi, Fachzi Fitri, Eti Yerizal, Hafni Bachtiar, and Elvie Zulka Kautzia Rachmawati. "Deteksi pepsin pada saliva pasien refluks laringofaring." Oto Rhino Laryngologica Indonesiana 48, no. 1 (June 28, 2018): 65. http://dx.doi.org/10.32637/orli.v48i1.257.
Full textAbstract:
Latar belakang: Refluks laringofaring (RLF) didefinisikan sebagai aliran balik cairan lambungke daerah laring dan faring, sehingga berkontak dengan saluran pencernaan dan pernapasan bagian atasyang menyebabkan keluhan suara serak, batuk, sensasi globus, throat clearing, dan post nasal drip. RLFmemberikan dampak negatif terhadap kualitas hidup terutama fungsi fisik dan emosi. Diagnosis RLFditegakkan dengan mengetahui riwayat penyakit, gejala klinis, pemeriksaan laringoskopi, serta menentukanadanya aliran balik cairan lambung ke laringofaring. Pemeriksaan ambulatory 24 hours double-probepHmetri merupakan baku emas untuk diagnosis RLF, tetapi pemeriksaan ini masih belum ideal. Salahsatu cara untuk menentukan RLF saat ini adalah dengan menentukan keberadaan pepsin pada laring danfaring, menggunakan metode Enzyme Linked Immunosorbent Assay (ELISA). Hal ini berdasarkan faktabahwa pepsin hanya dihasilkan pada lambung. Tujuan: Mengetahui karakteristik pasien, gambaran refluxsymptom index (RSI), gambaran reflux finding score (RFS) dan mengetahui kadar pepsin pada salivapasien RLF. Metode: Penelitian ini merupakan penelitian deskriptif untuk mengetahui gambaran hasilpemeriksaan RSI, RFS, dan kadar pepsin dalam saliva pasien RLF dengan metode ELISA di bagian TelingaHidung Tenggorok-Bedah Kepala dan Leher Rumah Sakit Dr. M. Djamil Padang, mulai Januari–Oktober2015. Hasil: Dari 30 responden yang terdiri dari perempuan 23 orang (76,7%), dan laki-laki 7 orang(23,3%), didapatkan kelompok usia terbanyak 48-57 tahun (40%), dengan rata-rata usia 47,2+12,06 tahun.Nilai rerata RSI 18,53+4,46, nilai rerata RFS 11,47+2,50, dan pada semua sampel didapatkan pepsin (+)dengan nilai rerata kadar pepsin dalam saliva responden 2,75+1,23 ng/ml. Kesimpulan: Pepsin terdeteksipada semua sampel saliva responden RLF. ABSTRACTBackground: Laryngopharyngeal reflux (LPR) is defined as the backflow of gastric contents intolarynx and pharynx areas, making contacts with upper digestive and respiratory tracks causing hoarseness,cough, globus sensation, throat clearing and post nasal drip. LPR has a negative impact on quality oflife. LPR diagnosis is confirmed by disease history, clinical symptoms, laryngoscopy examination andthe backflow of gastric fluid into laryngopharynx. Ambulatory examination of 24 hours double-probepHmetry is the gold standard for LPR diagnosis, although it is not yet ideal. To detect the presence ofpepsin in the larynx and pharynx using ELISA is now being used to determine LPR, based on the fact thatpepsin is only produced in the stomach. Purpose: To investigate patient characteristics, reflux symptomindex (RSI) and reflux finding score (RFS) descriptions, and pepsin level in the saliva of LPR patients.Methods: A descriptive research to describe RSI, RFS, and levels of pepsin in the saliva of LPR patientsusing ELISA at the Otorhinolaryngology Head and Neck Surgery Departement of Dr. M. Djamil Hospital,Padang, from January-October 2015. Results: Thirty respondents consisted of 23 females (76.7%),and 7 males (23.3%), revealed the largest age group was 48-57 years (40%), with an average age of 47.2+12.06 years. The average value of RSI 18.53+4.46, the average value of RFS 11.47+2.50, andpepsin result (+) in all samples, with an average value of pepsin level in respondents’ saliva 2.75+1.23ng ml. Conclusion: Pepsin was detected in all samples of LPR patients’ saliva.
50
Ratunanda, Sinta Sari, Billy Talakua, Teti Madiadipoera, Thaufiq Boesoirie, Ratna Anggraeni, and Rovina Ruslami. "Refluks Helicobacter pylori di mukosa hidung penderita rinosinusitis kronik disertai refluks laringofaring." Oto Rhino Laryngologica Indonesiana 48, no. 2 (January 30, 2019): 148. http://dx.doi.org/10.32637/orli.v48i2.272.
Full textAbstract:
Latar belakang: Rinosinusitis kronik masih menjadi problema di seluruh dunia. Faktor yang berasosiasi dengan Rinosinusitis Kronik (RSK) diduga multifaktorial, salah satunya adalah refluks laringofaring (RLF). Isi refluks cairan lambung antara lain adalah bakteri Helicobacter pylori (H. pylori) yang dengan patomekanisme refluks, diduga dapat mencapai mukosa laringofaring bahkan sampai mukosa sinonasal, dan menyebabkan RSK. Tujuan: Mendeteksi H. pylori di mukosa hidung akibat refluks pada penderita RSK disertai RLF. Bila terdeteksi H. pylori, tata laksana harus lebih komprehensif, sehingga diharapkan RSK menjadi terkontrol. Metode: Penelitian deskriptif untuk mengetahui ada tidaknya H. pylori di mukosa sinonasal penderita RSK dengan RLF. Deteksi H. pylori menggunakan teknik quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) dari bahan penyikatan mukosa hidung. Hasil: Didapatkan 86 orang penderita RSK disertai RLF, terdiri dari 30 (35%) pasien laki-laki dan 56 (65,0%) pasien wanita, dengan rerata usia 43,25±6,30 tahun. Keluhan RSK terbanyak adalah hidung tersumbat dengan skor VAS > 7 sebesar 76,8%. Skor nasoendoskopi RSK terbesar pada skor 2 untuk edema mukosa sebesar 65,3% dan skor 2 untuk sekret hidung sebesar 58,2%. Rata-rata skor gejala refluks (SGR) adalah 26,43±4,03 dan rata-rata total skor temuan refluks (STR) adalah 11,28±1,21. Hasil pemeriksaan deteksi H. pylori dengan qRT-PCR, 100% tidak menemukan H. pylori dari penyikatan mukosa hidung. Kesimpulan: Refluks berupa H. pylori tidak ditemukan pada mukosa hidung penderita RSK disertai RLF. Penelitian lebih lanjut diperlukan dengan menggunakan gabungan beberapa metode pemeriksaan bersamaan untuk deteksi H. pylori akibat refluks di mukosa sinonasal penderita RSK disertai RLF. Background: Chronic rhinosinusitis is presently still a worldwide problem. Assosiating factors to chronic rhinosinusitis (CRS) are multifactorial, one of them is laryngopharyngeal reflux (LPR). The gastric juice contains Helicobacter pylori (H. pylori), which by pathologic reflux could reach laryngopharyngeal and sinonasal area causing CRS. Purpose: To detect H. pylori in nasal mucosa caused by reflux, which suspected of causing CRS with LPR disease. Should H. pylori be found in nasal mucosa, the management of the disease must be comprehensive to enable controlling CRS. Methods: A descriptive study to detect H. pylori in nasal mucosa CRS with LPR patients, using Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) through nasal brushing. Results: Eighty-six CRS with LPR patients as study objects consisted of 30 (35%) male, and 56 (65%) female, the age mean was 43.25±6.3 years old. Visual Analoque Scale (VAS) score for nasal obstruction more than 7 was the highest complaint (76.8%). Nasal endoscopic score of mucosal edema (65.3%) and nasal discharge (58,2%) had score 2. The average total score reflux symptom index (RSI) was 26.43±4.03 and the total score reflux finding score (RFS) was 11.28±1.21. H. pylori detection found negative 100% in CRS with LPR specimens. Conclusion: This study did not find reflux containing H. pylori in nasal mucosa of CRS with LPR patients. Suggesting further study using simultaneously several methods to detect H. pylori in nasal mucosa CRS with LPR patients.