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Dissertations / Theses on the topic 'Ribbon molecules'

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Published: 10 December 2022
Last updated: 19 February 2023

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1

Khimich, Darina. "Molecular physiology of the inner hair cell ribbon synapses." [S.l.] : [s.n.], 2005. http://webdoc.sub.gwdg.de/diss/2005/khimich.

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2

Fallon, Philip Spencer. "The design, synthesis and applications of hydrogen bonded molecular assemblies." Thesis, University of Nottingham, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388247.

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3

Premarathna, Sineth Madushan. "Single Molecule Investigations of Sexiphenyl on Graphene Nano-Ribbons." Ohio University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1544128223705605.

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4

Obholzer, Nikolaus. "Molecular components of the hair cell synaptic vesicle cycle." [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:16-opus-79550.

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5

Houston, Oliver. "Investigating novel molecular regulators of the auditory ribbon synapses of mammalian inner hair cells." Thesis, University of Sheffield, 2015. http://etheses.whiterose.ac.uk/11421/.

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The auditory ribbon synapse is highly specialised to regulate the release of glutamate from IHCs and generate action potentials in auditory afferent fibres in response to small and graded changes in the receptor potential of IHCs. This is essential for maintaining the fidelity of auditory stimuli over wide range of frequencies and intensities. This study was aimed at identifying possible novel molecular regulators for the development and function of auditory ribbon synapses. I have used patch-clamp electrophysiology to record calcium currents and changes in membrane capacitance from IHCs to monitor vesicle fusion at the auditory ribbon synapse. The ubiquitously expressed calcium-sensor for vesicle priming, DOC2B was considered to be a potential priming factor at the auditory ribbon synapse. I have found that it is not involved in fast, calcium-dependent exocytosis of synaptic vesicles at the auditory ribbon synapse. The inositol 5’-phosphatase SynJ2 is required for the survival of auditory hair cells and is thought to regulate clathrin-mediated endocytosis. My results show that IHCs from SynJ2 KO mice display normal endocytic responses after exocytotic events and normal replenishment of the vesicle pools. Therefore, SynJ2 is not required for endocytosis, or vesicle recycling in IHCs. Connexins 26 and 30 are subunits of heteromeric gap-junctions and hemichannels in supporting cells of the cochlea. Connexin mutations cause over 50% of cases of non-syndromic hereditary deafness in humans. IHCs from mice with severely reduced connexin expression display larger calcium currents and smaller exocytotic responses. Therefore expression of connexins in the cochlea is essential for presynaptic function at the auditory ribbon synapse. Finally I have found that the transcriptional co-activator Wbp2 is not required for presynaptic function of mature IHCs, therefore Wbp2 is not involved in the transcription of key presynaptic molecules in IHCs.
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6

Richter, Katharina Natalia [Verfasser]. "The molecular anatomy of synaptic vesicle recycling at the hair cell ribbon synapse / Katharina Natalia Richter." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2019. http://d-nb.info/1215906129/34.

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7

Michanski, Susann [Verfasser], Tobias [Akademischer Betreuer] Moser, Tobias [Gutachter] Moser, and André [Gutachter] Fiala. "Molecular and structural investigation of assembly, maturation and heterogeneity of inner hair cell ribbon synapses / Susann Michanski ; Gutachter: Tobias Moser, André Fiala ; Betreuer: Tobias Moser." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2019. http://d-nb.info/1196873968/34.

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8

Schäfer, Philip Sudadyo. "Tuning of color and polarization of the fluorescence of nano-ribbons using laser microscopy and controlled self-assembly." Thesis, Bordeaux, 2016. http://www.theses.fr/2016BORD0435/document.

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Des matériaux ayant des propriétés émissives spécifiques peuvent être obtenus par l'organisation contrôlée de fluorophores aux échelles moléculaire, nano- et micro-métrique. Dans ce travail, l'émission de lumière bleue polarisée est obtenue par l'auto-assemblage hautement anisotrope de n-acènes alcoxylés en nano-rubans. Des techniques de microscopie de fluorescence ont été utilisées pour déterminer le mécanisme de leur croissance et ont été combinées à la cristallographie aux rayons X pour déterminer l'empilement moléculaire dans les nano-objets. L'étude a révélé que la formation des nano-rubans est induite non seulement par le mécanisme de maturation d'Ostwald très commun, mais aussi par une croissance par attachement orienté rarement démontré dans des systèmes organiques. En plus des techniques plus courantes, la microscopie en polarisation de fluorescence de molécules uniques a contribué à caraxctériser l'emplilement moléculaire, bien que les nano-objets à haute densité en chromophore constituent des échantillons très difficiles à étudier. Dans ce travail, les propriétés des nano-rubans ont été contrôlées au niveau microscopique par les conditions de croissance, ainsi que par l'addition de dopants. Ainsi, en combinant différentes molécules et une réaction photochimique sous microscopie, des rubans à motifs colorés sub-micrométriques ont été obtenus. Par ailleurs, l'assemblage orthogonal a été exploité pour développer des réseaux interpénétrés. Ces derniers se distinguent par une émission à double couleur, un transfert d'énergie entre objets et une électroluminescence aux jonctions
Materials with specific emissive properties can be obtained by the controlled organization of fluorophores at the molecular, nano- and microscales. In this work, polarized blue light emission is achieved by the highly anisotropic self-assembly of alkoxylated n-acenes into nano-ribbons. Fluorescence microscopy techniques were used to determine the growth mechanism and were combined to X-ray crystallography to determine the molecular packing in the nano-objects. The study revealed that the formation of the nano-ribbons is induced not only by the very common Ostwald ripening mechanism but also by an oriented attachnment growth, rarely observed with such evidence in organic systems. Besides more common techniques, single molecule fluorescence polarization microscopy contributed to characterize the molecular packing, although the nano-objects with high chromophore density represent very challenging samples. In this work, the properties of the nano-ribbons have been controlled at the microscopic level by the growth conditions, as well as by the addition of dopants Thereby, combining different molecules and photochemistry at the sub-micrometer scale under the microscope, colorful patterned ribbons could be obtained. In addition, orthogonal assembly was exploited to grow interpenetrated networks. The latter demonstrated dual color-emission, as well as inter-object energy transfer and electroluminescence at junctions
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9

Wang, Zhao. "Propriétés Electro-mécaniques des Nanotubes de Carbone." Phd thesis, Université de Franche-Comté, 2008. http://tel.archives-ouvertes.fr/tel-00352725.

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Le but de cette thèse était de modéliser la réponse mécanique de nanotubes de carbone à des champs électriques. Nous avons commencé par utiliser le potentiel AIREBO dans des simulations de dynamique moléculaire afin d'étudier l'élasticité non-linéaire et la limite de déformation en torsion de divers nanotubes, en fonction de leur longueur, rayon et chiralité. Nous trouvons notamment que le module d'Young effectif des tubes décroît d'autant plus vite que la chiralité est faible. D'autre part, nous montrons que la limite de l'énergie stockable par atome lors de la torsion d'un tube est d'autant plus grande que le diamètre est petit.

Nous modélisons ensuite, de façon atomistique, la distribution surfacique de charge électrique sur des nanotubes de carbone possédant une charge nette. Nous retrouvons notamment l'effet de pointe classique avec un très bon accord quantitatif avec des résultats expérimentaux obtenus par microscopie à force électrostatique.

Par combinaison des méthodes utilisées dans les études précédentes, nous simulons la déflection de nanotubes semi-conducteurs et métalliques par un champ électrique extérieur, dans une configuration de type interrupteur moléculaire. L'effet des caractéristiques géométriques des tubes et du champ sur cette déflection ont été systématiquement étudiés.

En outre, nous avons vu que des simulations de dynamique moléculaire avec le potentiel AIREBO permettent de retrouver quantitativement les énergies expérimentales d'adsorption du benzène, du naphtalène et d'anthracène sur le graphite. Ce type de simulation nous permet d'avancer sur la voie de la compréhension de la sélectivité de l'adsorption de certaines molécules surfactantes à plusieurs cycles benzéniques sur des nanotubes de chiralité donnée.
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10

Broach, William H. "Small RNAs of Shigella dysenteriae." Ohio University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1406822539.

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11

Neef, Jakob. "Molecular Physiology of the Ribbon Synapse." Doctoral thesis, 2010. http://hdl.handle.net/11858/00-1735-0000-0006-ADB1-D.

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12

(14010168), Mirta Golic. "The preparation of dienes and dienophiles containing nucleic acid bases." Thesis, 1999. https://figshare.com/articles/thesis/The_preparation_of_dienes_and_dienophiles_containing_nucleic_acid_bases/21397758.

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The work presented in this thesis deals initially with the synthesis of rigid polyalicyclic dienes and dienophiles with pyrimidine moieties inbuilt in a rigid fashion (building BLOCKS). This work has allowed the production of a new class of ribbon molecules with precisely defined size, shape and position of the pyrimidine ring. In the second stage of the project, an assessment of their ability to participate in cycloaddition reactions as pyrimidine building BLOCK* components was investigated.

2,4-Dimethoxy-1,3-diazaanthracene (I) has acted as the pyrimidine transfer reagent for preparing building BLOCKs. The Diels-Alder adducts IV and V (Scheme I), prepared by reaction of I with norbomadiene, are new pyrimidine dienophilic BLOCKs. Both I and 2,4-dichloro-1,3-diazaanthracene (II) were active in photochemical [4π+4π] cycloaddition reactions with cyclopentadiene to form a second class of building BLOCKs VII and VIII (Scheme I). In addition, the photodimerisation of I and II was studied and structures IX-XII assigned on the basis of spectral and X-ray method.

The 2,4-dichloro-photoadduct VIII is of particular importance for this work since it is easily hydrolysed (2M NaOH, 60 °C, overnight) to the corresponding uracil XIII In contrast, thermal adducts IV and V were very difficult to hydrolyse (NaOH fusion) to uracils XIV and XV (Figure I).

The availability of pyrimidine BLOCKs which contain a reactive π-bond, e.g. (IV, V, VII and VIII) has enabled us to employ 3,6-di(2'-pyridyl)-s-tetrazine (XVI) and ACE (Alkene plus Cyclobutene Epoxide) coupling methods to obtain precisely functionalised ribbon molecules in a direct, convergent synthetic strategy.

The synthesis of the bis-pyrimidines by coupling norbornene reagents using 3,6-di(2'-pyridyl)-s-tetrazine is illustrated in Scheme II. In the first step, s-tetrazine XVI was reacted with pyrimidine BLOCK V under basic conditions to generate the dihydropyridazine XVII. This diaza-1,3-diene was reacted with a further equivalent of V under high pressure conditions to yield the bis-pyrimidines XVIII and XIX, which were separated by radial chromatography. The same procedure was used to link pyrimiclines to other effectors by using alternative alkenes in the second step.

The ACE coupling protocol is illustrated by the reaction of alkene VIII with the dimethoxynaphthalene-containing epoxide XX (Scheme III). The reaction can be conducted under thermal or photochemical conditions and is considered to proceed via 1,3-dipolar intermediate formed by ring-opening of the epoxide C-C bond of XX (See Chapter 4).

Each class of coupled adduct could be hydrolysed to the corresponding uracil by using either acid (XXII) or base (XXIII) hydrolysis conditions, the choice depending on the structure of the molecule in question and its substituents.

The work presented in this thesis involves a deal of new work and has been instrumental in the development of the Lego®-based BLOCK assembly protocol for ribbon molecules construction.

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13

Khimich, Darina Wasylivna. "Molecular physiology of the inner hair cell ribbon synapses." Doctoral thesis, 2005. http://hdl.handle.net/11858/00-1735-0000-0006-B5F1-B.

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14

Neef, Jakob [Verfasser]. "Molecular physiology of the ribbon synapse / submitted by Jakob Neef." 2010. http://d-nb.info/1007527110/34.

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15

Frank, Thomas. "Investigating the Calcium Signaling at Ribbon Synapses." Doctoral thesis, 2010. http://hdl.handle.net/11858/00-1735-0000-0006-B50F-B.

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16

Khimich, Darina [Verfasser]. "Molecular physiology of the inner hair cell ribbon synapses / submitted by Khimich Darina." 2005. http://d-nb.info/977057151/34.

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17

Richter, Katharina Natalia. "The molecular anatomy of synaptic vesicle recycling at the hair cell ribbon synapse." Doctoral thesis, 2019. http://hdl.handle.net/21.11130/00-1735-0000-0005-12B0-F.

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18

Alpadi, Kannan [Verfasser]. "Molecular and functional characterization of Ribeye-MUNC119 interaction in the photoreceptor ribbon synapse / Kannan Alpadi." 2008. http://d-nb.info/1002765447/34.

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19

Michanski, Susann. "Molecular and structural investigation of assembly, maturation and heterogeneity of inner hair cell ribbon synapses." Doctoral thesis, 2018. http://hdl.handle.net/21.11130/00-1735-0000-0003-C1CD-C.

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20

Venkatesan, Jagadeesh Kumar [Verfasser]. "Molecular and functional characterization of Ribeye-GCAP2 interaction in the photoreceptor ribbon synapse / Jagadeesh Kumar Venkatesan." 2010. http://d-nb.info/1011158906/34.

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21

Sendin, Gaston Carlos. "Maturation of ribbon synapses in hair cells is driven by thyroid hormone." Doctoral thesis, 2007. http://hdl.handle.net/11858/00-1735-0000-0006-B36B-F.

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22

Krinner, Stefanie. "Molecular physiology of synaptic sound encoding at the first auditory synapse." Doctoral thesis, 2017. http://hdl.handle.net/11858/00-1735-0000-002E-E396-A.

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23

Revelo, Nuncira Natalia Hasel. "A novel membrane-binding probe for the morphological and molecular characterization of synaptic vesicle recycling pathways." Doctoral thesis, 2014. http://hdl.handle.net/11858/00-1735-0000-0022-5FDB-C.

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24

Kamin, Dirk. "Synaptic vesicle recycling investigated by high-resolution microscopy in a conventional and a sensory synapse." Doctoral thesis, 2011. http://hdl.handle.net/11858/00-1735-0000-000D-F0BF-1.

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