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1

Ramana, V. Venkata, S. Kalyana Chakravarthy, P. Shalem Raj, B. Vinay Kumar, E. Shobha, E. V. V. Ramaprasad, Ch Sasikala, and Ch V. Ramana. "Descriptions of Rhodopseudomonas parapalustris sp. nov., Rhodopseudomonas harwoodiae sp. nov. and Rhodopseudomonas pseudopalustris sp. nov., and emended description of Rhodopseudomonas palustris." International Journal of Systematic and Evolutionary Microbiology 62, Pt_8 (August 1, 2012): 1790–98. http://dx.doi.org/10.1099/ijs.0.026815-0.

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Four strains (JA310T, JA531T, JA447 and JA490) of red to reddish brown pigmented, rod-shaped, motile and budding phototrophic bacteria were isolated from soil and freshwater sediment samples from different geographical regions of India. All strains contained bacteriochlorophyll a and carotenoids of the spirilloxanthin series. The major cellular fatty acid of strains JA310T and JA531T was C18 : 1ω7c, the quinone was Q-10 and polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an aminohopanoid and an unidentified aminolipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that all strains clustered with species of the genus Rhodopseudomonas in the class Alphaproteobacteria . Strains JA531T, JA447 and JA490 were genotypically (>80 % related based on DNA–DNA hybridization) and phenotypically closely related to each other and the three strains were distinct from strain JA310T (33 % related). Furthermore, all four strains had less than 48 % relatedness (DNA–DNA hybridization) with type strains of members of the genus Rhodopseudomonas , i.e. Rhodopseudomonas palustris ATCC 17001T, Rhodopseudomonas faecalis JCM 11668T and Rhodopseudomonas rhenobacensis DSM 12706T. The genomic DNA G+C contents of strains JA310T and JA531T were 63.8 and 62.4 mol%, respectively. On the basis of phenotypic, chemotaxonomic and molecular genetic evidence, it is proposed that strains JA310T ( = NBRC 106083T = KCTC 5839T) and JA531T ( = NBRC 107575T = KCTC 5841T) be classified as the type strains of two novel species of the genus Rhodopseudomonas , Rhodopseudomonas parapalustris sp. nov. and Rhodopseudomonas harwoodiae sp. nov., respectively. In addition, we propose that strain DSM 123T ( = NBRC 100419T) represents a novel species, Rhodopseudomonas pseudopalustris sp. nov., since this strain differs genotypically and phenotypically from R. palustris ATCC 17001T and other members of the genus Rhodopseudomonas . An emended description of R. palustris is also provided.
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2

Harwood, Caroline S. "Rhodopseudomonas palustris." Trends in Microbiology 30, no. 3 (March 2022): 307–8. http://dx.doi.org/10.1016/j.tim.2021.12.001.

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3

Huang, Xuejiao, Jiupai Ni, Chong Yang, Mi Feng, Zhenlun Li, and Deti Xie. "Efficient Ammonium Removal by Bacteria Rhodopseudomonas Isolated from Natural Landscape Water: China Case Study." Water 10, no. 8 (August 20, 2018): 1107. http://dx.doi.org/10.3390/w10081107.

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In this study, we isolated a strain of photosynthetic bacteria from landscape water located in Southwest University, Chongqing, China, and named it Smobiisys501. Smobiisys501 was Rhodopseudomonas sp. according to its cell morphological properties and absorption spectrum analysis of living cells. The analysis of the 16S rDNA amplification sequence with specific primers of photosynthetic bacteria showed that the homology between Smobiisys501 and Rhodopseudomonas sp. was 100%, and the alignment results of protein sequences of the bacterial chlorophyll Y subunit showed that Smobiisys501 and Rhodopseudomonas palustris were the most similar, with a similarity of more than 92%. However, Smobiisys501 could not utilize glucose and mannitol as a carbon source and had a low fatty acid content, which were different from the related strains of the genus Rhodopseudomonas. Moreover, the DNA-DNA relatedness was only 42.2 ± 3.3% between Smobiisys501 and the closest strain Rhodopseudomonas palustris. Smobiisys501 grew optimally at 30 °C and pH 7.0 in the presence of yeast extract, and it could efficiently remove ammonium (99.67% removal efficiency) from synthetic ammonium wastewater. All the results indicated that Smobiisys501 was a novel species of Rhodopseudomonas, with the ability to remove ammonium.
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4

Xu, Cheng Bin, Xue Kai Sun, Yao Yao Li, Yan Gang Wang, and Xue Lian Meng. "Study on Optimization of the Culture Conditions for Four Rhodopseudomonas spp." Advanced Materials Research 393-395 (November 2011): 976–79. http://dx.doi.org/10.4028/www.scientific.net/amr.393-395.976.

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Rhodopseudomonas sp. could be used in wastewater treatment and aquiculture, extensively. Four photosynthetic bacterium Rhodopseudomonas spp., QDS2, QDS3, QDS4 and QDS9, were isolated and screened from the bottom sludge of Qingdao coast. In order to get a mass of PSB, we successfully optimized the culture conditions of four strains by orthogonal experiment and obtained the best culture conditions. The research could provide the bases for the applying of high-effective PSB (Rhodopseudomonas sp).
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5

Humphrey, Christine E., Nicole Burnett, Shivangi Dubey, and John A. Kyndt. "Genomic and Phylogenetic Characterization of Rhodopseudomonas infernalis sp. nov., Isolated from the Hell Creek Watershed (Nebraska)." Microorganisms 10, no. 10 (October 13, 2022): 2024. http://dx.doi.org/10.3390/microorganisms10102024.

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The genus Rhodopseudomonas is known for its versatile metabolic capabilities and has been proposed to be used in a wide variety of innovative applications, ranging from biohydrogen and electricity production, bioremediation and as biostimulant in agriculture. Here, we report the isolation, characterization and genome sequence analysis of a novel Rhodopseudomonas species, strain HC1, isolated from the Hell Creek urban native restoration area. Whole genome-based analysis, average nucleotide identity (ANI) comparison, and growth characteristics identified this isolate as a new species of the Rhodopseudomonas genus, for which we propose the name Rhodopseudomonas infernalis sp. nov. Besides containing several nitrogenases for nitrogen fixation and hydrogen production, the HC1 genome encodes a unique gene cluster, not found in any other Rhodopseudomonas species, which encodes genes for the degradation of yet-unidentified aromatic PCB-type chemicals with potentially interesting biotechnological applications. The genomic features of Rps. infernalis HC1 indicate that it plays a positive role in the degradation of anthropogenic substances and aids the restoration of the Hell Creek watershed by contributing to N2 and carbon fixation and plant growth; however, the genome also contains several antibiotic resistance genes, indicating a broad range of antibiotic resistance in this environmental isolate.
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6

Kumar, B. Vinay, E. V. V. Ramprasad, Ch Sasikala, and Ch V. Ramana. "Rhodopseudomonas pentothenatexigens sp. nov. and Rhodopseudomonas thermotolerans sp. nov., isolated from paddy soils." International Journal of Systematic and Evolutionary Microbiology 63, Pt_1 (January 1, 2013): 200–207. http://dx.doi.org/10.1099/ijs.0.038620-0.

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Two strains (JA575T and JA576T) of orange- to pink-pigmented, rod-shaped, motile and budding phototrophic bacteria were isolated from paddy soils. Both strains contained bacteriochlorophyll a and carotenoids of spirilloxanthin series. Both strains had C18 : 1ω7c as the major cellular fatty acid, ubiquinone-10 (Q10) as the main quinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine as polar lipids. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that both strains clustered with species of the genus Rhodopseudomonas in the class Alphaproteobacteria . Strains JA575T and JA576T were genotypically (<35 % DNA–DNA relatedness) and phenotypically distinct from each other. Further, both strains showed less than 48 % DNA–DNA relatedness with the type strains of all recognized species of the genus Rhodopseudomonas . The molecular evidence is supported by phenotypic evidence. It is proposed that strains JA575T and JA576T be classified as representing two novel species of the genus Rhodopseudomonas with the species names Rhodopseudomonas pentothenatexigens sp. nov. and Rhodopseudomonas thermotolerans sp. nov., respectively. The type strains of the proposed novel species are JA575T ( = NBRC 108862T = KCTC15143T) and JA576T ( = NBRC 108863T = KCTC 15144T), respectively.
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7

Zhang, Dong Chen, Zhi Xiang Hou, and Tao Wang. "Study on Rhodopseudomonas Spheroides as Coal Microbial Flocculant." Advanced Materials Research 550-553 (July 2012): 1090–93. http://dx.doi.org/10.4028/www.scientific.net/amr.550-553.1090.

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Orthogonal test was employed to inspect the flocculation of Rhodopseudomonas spheroides in this text. The result showed that Rhodopseudomonas spheroides has good performance in flocculation effect of coal slurry. The hightest flocculation ratio is 93.4%. The optimum technological combination of flocculation test has been obtained:incubation time:12h,Coagulation aid dosage:2mL,Bacterium fluid amount:2mL,The categories of flocculants: the cell homogenates,pH:7.The FTIR analysis showed that the extractive of Rhodopseudomonas spheroides contain a large number of acidic Polysaccharides which has flocculent effect component.The main flocculation is “adsorption-bridge” between Molecular bioflocculant and coal slurry.
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8

Hiraishi, A., T. S. Santos, J. Sugiyama, and K. Komagata. "Rhodopseudomonas rutila Is a Later Subjective Synonym of Rhodopseudomonas palustris." International Journal of Systematic Bacteriology 42, no. 1 (January 1, 1992): 186–88. http://dx.doi.org/10.1099/00207713-42-1-186.

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9

Imhoff, Johannes F., Terrance E. Meyer, and John Kyndt. "Genomic and genetic sequence information of strains assigned to the genus Rhodopseudomonas reveal the great heterogeneity of the group and identify strain Rhodopseudomonas palustris DSM 123T as the authentic type strain of this species." International Journal of Systematic and Evolutionary Microbiology 70, no. 6 (June 1, 2020): 3932–38. http://dx.doi.org/10.1099/ijsem.0.004077.

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The genus Rhodopseudomonas , containing purple nonsulfur photosynthetic Proteobacteria, has a number of strains that belong to different species, although many of them are collectively called Rhodopseudomonas palustris . The type species R. palustris and closely related species are the focus of this paper. The comparison of available genome sequences indicate that the following Rhodopseudomonas species are well recognized: R. palustris (strains ATH 2.1.6T=DSM 123T=NBRC 100419T and BisB5), Rhodopseudomonas rutila (strains R1T, DSM 126, CGA009, ATH 2.1.37, Eli 1980, ATCC 17001 and TIE1), Rhodopseudomonas pentothenatexigens JA575T and Rhodopseudomonas faecalis JCM 11668T. Other strains for which genome sequences are available are distinct from these four species. Evidence is presented that R. palustris strain ATH 2.1.6T–KCM as obtained directly from the van Niel collection by one of us (T.E.M.) is identical to the DSMZ deposit DSM 123T of ATH 2.1.6T, but not to the deposit at ATCC 17001. The amino acid sequences of the cytochromes C2 and C556 from R. palustris strain ATH 2.1.6T–KCM are in complete agreement with the translated genome sequences of R. palustris DSM 123T. In addition, the 16S rRNA gene sequence of R. palustris NBRC 100419T completely matches that of strain DSM 123T. In conclusion, the type strain of R. palustris ATH 2.1.6T is correctly represented by DSM 123T and NBRC 100419T. However, the deposit at ATCC 17001 has properties that do not conform with properties of authentic R. palustris , but rather indicate that this is a strain of R. rutila . The previously suggested assignment of the type strain of R. palustris DSM 123T to the new species R. pseudopalustris was incorrect because strain DSM 123T is the authentic type strain of R. palustris .
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10

Campbell, J. I. A., S. Scahill, T. Gibson, and R. P. Ambler. "The phototrophic bacterium Rhodopseudomonas capsulata sp108 encodes an indigenous class A β-lactamase." Biochemical Journal 260, no. 3 (June 15, 1989): 803–12. http://dx.doi.org/10.1042/bj2600803.

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The nucleotide sequence of a 2.37 kb DNA fragment derived from cloning a total DNA digest of Rhodopseudomonas capsulata sp108 was determined. The DNA codes for a beta-lactamase, a protein showing sequence similarity to the ampR protein of Enterobacter cloacae and an unidentified open reading frame. Hybridization experiments with a probe carrying DNA from within the beta-lactamase gene suggests a chromosomal location for the coding sequences in strain sp108 and in sp109, a penicillin-sensitive revertant of sp108 in which the enzyme is not inducible. A protein-sequence comparison of the deduced amino acid sequence of the Rps. capsulata beta-lactamase indicates that it is a Class A enzyme and that its sequence can be aligned with those of the characterized beta-lactamases from Staphylococcus aureus, Bacillus licheniformis and the Escherichia coli plasmid (R-TEM enzyme), with only a few insertions or deletions. The corresponding DNA sequence is, however, characteristically rhodopseudomonad, suggesting that it is not a recently transposed gene.
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11

Oda, Yasuhiro, Wouter Wanders, Louis A. Huisman, Wim G. Meijer, Jan C. Gottschal, and Larry J. Forney. "Genotypic and Phenotypic Diversity within Species of Purple Nonsulfur Bacteria Isolated from Aquatic Sediments." Applied and Environmental Microbiology 68, no. 7 (July 2002): 3467–77. http://dx.doi.org/10.1128/aem.68.7.3467-3477.2002.

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ABSTRACT To assess the extent of genotypic and phenotypic diversity within species of purple nonsulfur bacteria found in aquatic sediments, a total of 128 strains were directly isolated from agar plates that had been inoculated with sediment samples from Haren and De Biesbosch in The Netherlands. All isolates were initially characterized by BOX-PCR genomic DNA fingerprinting, and 60 distinct genotypes were identified. Analyses of 16S rRNA gene sequences of representatives of each genotype showed that five and eight different phylotypes of purple nonsulfur bacteria were obtained from the Haren and De Biesbosch sites, respectively. At the Haren site, 80.5% of the clones were Rhodopseudomonas palustris, whereas Rhodoferax fermentans and Rhodopseudomonas palustris were numerically dominant at the De Biesbosch site and constituted 45.9 and 34.4% of the isolates obtained, respectively. BOX-PCR genomic fingerprints showed that there was a high level of genotypic diversity within each of these species. The genomic fingerprints of Rhodopseudomonas palustris isolates were significantly different for isolates from the two sampling sites, suggesting that certain strains may be endemic to each sampling site. Not all Rhodopseudomonas palustris isolates could degrade benzoate, a feature that has previously been thought to be characteristic of the species. There were differences in the BOX-PCR genomic fingerprints and restriction fragment length polymorphisms of benzoate-coenzyme A ligase genes and form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) genes between benzoate-degrading and non-benzoate-degrading genotypes. The ability to distinguish these two Rhodopseudomonas palustris groups based on multiple genetic differences may reflect an incipient speciation event resulting from adaptive evolution to local environmental conditions.
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12

Touloupakis, Eleftherios, Eleni G. Poloniataki, Martina Casciana, Demetrios F. Ghanotakis, and Pietro Carlozzi. "Poly-β-Hydroxybutyrate Production by Rhodopseudomonas sp. Grown in Semi-Continuous Mode in a 4 L Photobioreactor." Symmetry 13, no. 9 (September 2, 2021): 1609. http://dx.doi.org/10.3390/sym13091609.

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The synthesis of polyhydroxybutyrate (PHB) by photosynthetic non-sulfur bacteria is a potential approach for producing biodegradable plastics. In this work, acetate was used as a single carbon source to study the effect on PHB formation in Rhodopseudomonas sp. cultured in a cylindrical four-liter photobioreactor under semi-continuous mode. The cultivation process is divided into a symmetrical growth phase and a PHB accumulation phase separated temporally. The symmetrical growth phase (nutrient sufficient conditions) was followed by a sulfur-limited phase to promote PHB accumulation. The main novelty is the progressive lowering of the sulfur concentration into Rhodopseudomonas culture, which was obtained by two concomitant conditions: (1) sulfur consumption during the bacterial growth and (2) semi-continuous growth strategy. This caused a progressive lowering of the sulfur concentration into Rhodopseudomonas culturedue to the sulfur-free medium used to replace 2 L of culture (50% of the total) that was withdrawn from the photobioreactor at each dilution. The PHB content ranged from 9.26% to 15.24% of cell dry weight. At the steady state phase, the average cumulative PHB was >210 mg/L. Sulfur deficiency proved to be one of the most suitable conditions to obtain high cumulative PHB in Rhodopseudomonas culture.
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13

Juknat, A. A., M. L. Kotler, G. E. Koopmann, and A. M. del C. Batlle. "Porphobilinogenase from Rhodopseudomonas palustris." Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 92, no. 2 (January 1989): 291–95. http://dx.doi.org/10.1016/0305-0491(89)90280-0.

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14

Tegtmeyer, B., J. Weckesser, H. Mayer, and J. F. Imhoff. "Chemical composition of the lipopolysaccharides of Rhodobacter sulfidophilus, Rhodopseudomonas acidophila, and Rhodopseudomonas blastica." Archives of Microbiology 143, no. 1 (October 1985): 32–36. http://dx.doi.org/10.1007/bf00414764.

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15

Matsuura, Katsumi, and Keizo Shimada. "Cytochromes functionally associated to photochemical reaction centers in Rhodopseudomonas palustris and Rhodopseudomonas acidophila." Biochimica et Biophysica Acta (BBA) - Bioenergetics 852, no. 1 (November 1986): 9–18. http://dx.doi.org/10.1016/0005-2728(86)90050-2.

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16

Okubo, Yoko, Hiroyuki Futamata, and Akira Hiraishi. "Characterization of Phototrophic Purple Nonsulfur Bacteria Forming Colored Microbial Mats in a Swine Wastewater Ditch." Applied and Environmental Microbiology 72, no. 9 (September 2006): 6225–33. http://dx.doi.org/10.1128/aem.00796-06.

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ABSTRACT The community structure of pink-colored microbial mats naturally occurring in a swine wastewater ditch was studied by culture-independent biomarker and molecular methods as well as by conventional cultivation methods. The wastewater in the ditch contained acetate and propionate as the major carbon nutrients. Thin-section electron microscopy revealed that the microbial mats were dominated by rod-shaped cells containing intracytoplasmic membranes of the lamellar type. Smaller numbers of oval cells with vesicular internal membranes were also found. Spectroscopic analyses of the cell extract from the biomats showed the presence of bacteriochlorophyll a and carotenoids of the spirilloxanthin series. Ubiquinone-10 was detected as the major quinone. A clone library of the photosynthetic gene, pufM, constructed from the bulk DNA of the biomats showed that all of the clones were derived from members of the genera Rhodobacter and Rhodopseudomonas. The dominant phototrophic bacteria from the microbial mats were isolated by cultivation methods and identified as being of the genera Rhodobacter and Rhodopseudomonas by studying 16S rRNA and pufM gene sequence information. Experiments of oxygen uptake with lower fatty acids revealed that the freshly collected microbial mats and the Rhodopseudomonas isolates had a wider spectrum of carbon utilization and a higher affinity for acetate than did the Rhodobacter isolates. These results demonstrate that the microbial mats were dominated by the purple nonsulfur bacteria of the genera Rhodobacter and Rhodopseudomonas, and the bioavailability of lower fatty acids in wastewater is a key factor allowing the formation of visible microbial mats with these phototrophs.
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17

Strid, Åke, Pål Nyrén, Lars P. Christensen, Tove Thomasen, Ewa Huskowska, Lija Tekenbergs-Hjelte, Kristina Ohlson, et al. "F1-ATPase from Rhodopseudomonas blastica." Acta Chemica Scandinavica 43 (1989): 1007–8. http://dx.doi.org/10.3891/acta.chem.scand.43-1007.

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18

Kitao, Osamu. "Molecular Structure of Rhodopseudomonas viridis." Journal of Computer Aided Chemistry 1 (2000): 57–62. http://dx.doi.org/10.2751/jcac.1.57.

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19

Linscheid, Michael, Bernd W. K. Diehl, Monika Övermöhle, Iris Riedl, and Ernst Heinz. "Membrane lipids of Rhodopseudomonas viridis." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 1347, no. 2-3 (August 1997): 151–63. http://dx.doi.org/10.1016/s0005-2760(97)00065-9.

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20

Zhang, Chun-Ting, and Ren Zhang. "Genomic islands in Rhodopseudomonas palustris." Nature Biotechnology 22, no. 9 (September 2004): 1078–79. http://dx.doi.org/10.1038/nbt0904-1078b.

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21

Gorham, H., and C. S. Dow. "A bacteriophage of Rhodopseudomonas blastica." Microbiology 142, no. 4 (April 1, 1996): 979–83. http://dx.doi.org/10.1099/00221287-142-4-979.

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22

Henseler, A. "Soluble cytochromes of Rhodopseudomonas marina." FEMS Microbiology Letters 33, no. 1 (January 1986): 1–8. http://dx.doi.org/10.1016/0378-1097(86)90175-8.

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23

Genthner, Barbara R. Sharak, and Judy D. Wall. "Ammonium uptake in Rhodopseudomonas capsulata." Archives of Microbiology 141, no. 3 (April 1985): 219–24. http://dx.doi.org/10.1007/bf00408062.

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24

Takakuwa, S. "Nickel uptake in Rhodopseudomonas capsulata." Archives of Microbiology 149, no. 1 (November 1987): 57–61. http://dx.doi.org/10.1007/bf00423137.

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25

HOSHINO, Yasuo, and Toshio SATOH. "Dependence on calcium ions of gelatin hydrolysis by Rhodopseudomonas capsulata but not Rhodopseudomonas gelatinosa." Agricultural and Biological Chemistry 49, no. 11 (1985): 3331–32. http://dx.doi.org/10.1271/bbb1961.49.3331.

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26

Wang, Bo, Kemeng Xiao, Zhifeng Jiang, Jianfang Wang, Jimmy C. Yu, and Po Keung Wong. "Biohybrid photoheterotrophic metabolism for significant enhancement of biological nitrogen fixation in pure microbial cultures." Energy & Environmental Science 12, no. 7 (2019): 2185–91. http://dx.doi.org/10.1039/c9ee00705a.

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Na, Yang-Key, Kwang-Chul Na, Byeong-Sam Kim, Kyong-Ju Choi, Gil-Ho Shin, and Wol-Soo Kim*. "Effect of Rhodopseudomonas p. on Tree Growth and Fruit Quality of `Niitaka' Pear." HortScience 39, no. 4 (July 2004): 814E—814. http://dx.doi.org/10.21273/hortsci.39.4.814e.

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The objective of this study was to determine the effect of Rhodopseudomonas p., which isolated from domestic soil on the density of microorganism in soil, leaf and SSC in pear (Pyrus pyrifolia) fruit. The solution of Rhodopseudomonas p. was sprayed over canopy and fertigated around trunk in 12-year-old `Niitaka' pear with Y-training system. The spray and fertigation were performed 11 times at 15-day intervals from 22 Apr. to 22 Sept. with 200 times diluted solution (v/v). The width and length of treated leaf was increased by 1.4 mm and 0.9 mm compared to them of control. There was no difference in chlorophyll a content between treatments. The treatment increased SSC relatively to control by 0.7% and fruit firmness by 0.29 higher but there was no difference fruit weight. Hinter `L' value was higher by 3.5 and Hunter `a' lower by 0.2 compared to control so fruit color was more clean than control. In fertigation treatment, the density of microorganism and fluorescent bacteria in soil showed increasing tendency compared to control but the opposite in bacteria. These results suggested that the spray and fertigation of Rhodopseudomonas p. solution improve SSC and color of pear fruit.
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Wang, Qing, Lijun Shen, Zhenzhen Zhao, Hai Yan, Qianqian Xu, Chunhua Yin, Xiaolu Liu, Haiyang Zhang, and Yang Liu. "Efficient Culture of Rhodopseudomonas Palustris Using Landfill Leachate." Journal of Pure and Applied Microbiology 12, no. 4 (December 30, 2018): 1679–85. http://dx.doi.org/10.22207/jpam.12.4.01.

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29

Dmitrieva, Anastasia, Elizaveta Faskhutdinova, Timothy Larichev, Natalia Velichkovich, Veronika Boger, and Larisa Aksenova. "Environmentally friendly energy, extremophilic microorganisms, enzymatic activity, microbial fuel cell, hard-to-decompose substrates." Food Processing: Techniques and Technology 54, no. 1 (March 28, 2024): 27–36. http://dx.doi.org/10.21603/2074-9414-2024-1-2486.

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Traditional energy sources pollute the environment. Microbial fuel cells are an alternative energy source that can reduce the environmental burden. Microbial fuel cells also remove recalcitrant wastes from wastewater. This research featured the enzymatic potential of microbial isolates obtained from the Abakan Arzhan thermal spring. The study involved isolates of the genera Geobacter, Thermomonas, and Rhodopseudomonas. The keratinolytic analysis was in line with State Standard R 55987-2014. The chitinolytic activity was determined by injecting a bacterial suspension on Petri dishes with a chitin-containing medium. The lipolytic analysis involved cultivating the isolates in Stern’s glycerol fuchsin broth. The xylan hydrolysis depended on the reducing sugars. The cellulase activity was measured according to the standard method recom mended by the International Union of Pure and Applied Chemistry (IUPAC). The catalase potential was evaluated by the gasometric method on 1% gasoline media. The optimal parameters of consortium cultivation were determined by the voltage generated. The Geobacter isolate had the maximal keratinolytic activity while the Thermomonas isolate demonstrated the maximal protein hydrolysis (80.1 ± 1.5%). Both Geobacter and Rhodopseudomonas showed good lytic activity against chitin with the lysis zone of ≥ 3 mm. The Geobacter isolate demonstrated as many as 350 units of xylanase activity and 365 units of cellulase activity; Ther momonas had 350 units of xylanase activity and 360 units of cellulase activity; Rhodopseudomonas showed 310 units of xylanase activity and 304 units of cellulase activity. The maximal catalase properties belonged to Geobacter (1.40 units) and Thermomonas (1.38 units). The maximal energy generation by bacterial consortia occurred at pH 8 and 45°C after 48 h of cultivation. In this research, isolates of the genera Geobacter, Thermomonas, and Rhodopseudomonas from the Abakan Arzhan thermal spring were able to remove recalcitrant components, thus demonstrating good prospects for biological treatment of industrial wastewater.
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MÜLLER, Horst, Hartmut NEUFANG, and Karl KNOBLOCH. "Purification and Properties of the Coupling-Factor ATPases F1 from Rhodopseudomonas palustris and Rhodopseudomonas sphaeroides." European Journal of Biochemistry 127, no. 3 (March 3, 2005): 559–66. http://dx.doi.org/10.1111/j.1432-1033.1982.tb06908.x.

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31

Coleman, Tom, Rebecca R. Chao, John B. Bruning, James J. De Voss, and Stephen G. Bell. "CYP199A4 catalyses the efficient demethylation and demethenylation of para-substituted benzoic acid derivatives." RSC Advances 5, no. 64 (2015): 52007–18. http://dx.doi.org/10.1039/c5ra08730a.

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CYP199A4, a cytochrome P450 enzyme from Rhodopseudomonas palustris HaA2, is able to efficiently demethylate a range of benzoic acids at the para-position. It can also catalyse demethenylation reactions.
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32

Lang, F. S., and D. Oesterhelt. "Gene transfer system for Rhodopseudomonas viridis." Journal of Bacteriology 171, no. 8 (1989): 4425–35. http://dx.doi.org/10.1128/jb.171.8.4425-4435.1989.

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33

Moody, M. D., and H. A. Dailey. "Ferric iron reductase of Rhodopseudomonas sphaeroides." Journal of Bacteriology 163, no. 3 (1985): 1120–25. http://dx.doi.org/10.1128/jb.163.3.1120-1125.1985.

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34

H�dig, Hendrik, Norbert Kaufmann, and Gerhart Drews. "Respiratory deficient mutants of Rhodopseudomonas capsulata." Archives of Microbiology 145, no. 4 (September 1986): 378–85. http://dx.doi.org/10.1007/bf00470875.

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35

Kundu, Balaram, and D. J. D. Nicholas. "Denitrification in Rhodopseudomonas sphaeroides f. denitrificans." Archives of Microbiology 141, no. 1 (February 1985): 57–62. http://dx.doi.org/10.1007/bf00446740.

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36

Gallili, Gilad E., and Richard I. Mateles. "Production of catalase from Rhodopseudomonas spheroides." Journal of Applied Chemistry and Biotechnology 27, no. 1 (May 29, 2007): 110–16. http://dx.doi.org/10.1002/jctb.5020270116.

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37

Wang, Zijuan, Dong Gao, Hao Geng, and Chengfen Xing. "Enhancing hydrogen production by photobiocatalysis through Rhodopseudomonas palustris coupled with conjugated polymers." Journal of Materials Chemistry A 9, no. 35 (2021): 19788–95. http://dx.doi.org/10.1039/d1ta01019k.

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Herein, a feasible and simple bio-hybrid complex based on water-soluble conjugated polymers and Rhodopseudomonas palustris (R. palustris), one kind of photosynthetic bacteria, was constructed for enhancing photocatalytic hydrogen production.
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38

Nalvothula, Raju, Surekha Challa, Vidyullatha Peddireddy, Ramchander Merugu, M. P. Pratap Rudra, Abed Alataway, Ahmed Z. Dewidar, and Hosam O. Elansary. "Isolation, Molecular Identification and Amino Acid Profiling of Single-Cell-Protein-Producing Phototrophic Bacteria Isolated from Oil-Contaminated Soil Samples." Molecules 27, no. 19 (September 23, 2022): 6265. http://dx.doi.org/10.3390/molecules27196265.

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In the current study, soil samples were gathered from different places where petrol and diesel filling stations were located for isolation of photosynthetic bacteria under anaerobic conditions using the paraffin wax-overlay pour plate method with Biebl and Pfennig’s medium. The three isolated strains were named Rhodopseudomonas palustris SMR 001 (Mallapur), Rhodopseudomonas palustris NR MPPR (Nacahram) and Rhodopseudomonas faecalis N Raju MPPR (Karolbagh). The morphologies of the bacteria were examined with a scanning electron microscope (SEM). The phylogenetic relationship between R. palustris strains was examined by means of 16S rRNA gene sequence analysis using NCBI-BLAST search and a phylogenetic tree. The sequenced data for R. palustris were deposited with the National Centre for Biotechnology Research (NCBI). The total amino acids produced by the isolated bacteria were determined by HPLC. A total of 14 amino acids and their derivatives were produced by the R. palustris SMR 001 strain. Among these, carnosine was found in the highest concentration (8553.2 ng/mL), followed by isoleucine (1818.044 ng/mL) and anserine (109.5 ng/mL), while R. palustris NR MPPR was found to produce 12 amino acids. Thirteen amino acids and their derivatives were found to be produced from R. faecalis N Raju MPPR, for which the concentration of carnosine (21601.056 ng/mL) was found to be the highest, followed by isoleucine (2032.6 ng/mL) and anserine (227.4 ng/mL). These microbes can be explored for the scaling up of the process, along with biohydrogen and single cell protein production.
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39

Wang, Yiming, Chen Ma, Jin Zhang, Jin Tong, Rui Gao, and Wei Dai. "Investigation of cobalt bioaccumulation in Rhodopseudomonas palustris by proteomics analysis." Analytical Methods 11, no. 33 (2019): 4280–88. http://dx.doi.org/10.1039/c9ay00406h.

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Cobalt bioaccumulation by Rhodopseudomonas palustris (R. palustris), which has different modes of metabolism for tolerating heavy metal stress, was investigated using proteomics analysis under aerobic-dark conditions in the presence of a high cobalt concentration.
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40

Touloupakis, Eleftherios, Angeliki Chatziathanasiou, Demetrios F. Ghanotakis, Pietro Carlozzi, and Isabella Pecorini. "Hydrogen Production by Immobilized Rhodopseudomonas sp. Cells in Calcium Alginate Beads." Energies 15, no. 22 (November 9, 2022): 8355. http://dx.doi.org/10.3390/en15228355.

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The present investigation concerns the potentiality of Rhodopseudomonas sp. cells to produce clean energy such as molecular hydrogen (H2). The abovementioned goal could be reached by improving the capability of purple non-sulfur bacteria to produce H2 via a photofermentative process through the enzyme nitrogenase. Rhodopseudomonas sp. cells were immobilized in calcium alginate gel beads and cultured in a cylindrical photobioreactor at a working volume of 0.22 L. The semi-continuous process, which lasted for 11 days, was interspersed with the washing of the beads with the aim of increasing the H2 production rate. The maximum H2 production rate reached 5.25 ± 0.93 mL/h with a total output of 505 mL. The productivity was 40.9 μL (of H2)/mg (of cells)/h or 10.2 mL (of H2)/L (of culture)/h with a light conversion efficiency of 1.20%.
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41

Tarabas, O., S. Hnatush, B. Оstash, G. Мutenko, and O. Koshla. "IDENTIFICATION OF PURPLE NON- SULFUR BACTERIA OF RHODOPSEUDOMONAS SP. Ya-2016." Visnyk of Lviv University. Biological series, no. 75 (February 1, 2017): 140–45. http://dx.doi.org/10.30970/vlubs.2017.75.15.

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42

Allen, J. P., G. Feher, T. O. Yeates, D. C. Rees, J. Deisenhofer, H. Michel, and R. Huber. "Structural homology of reaction centers from Rhodopseudomonas sphaeroides and Rhodopseudomonas viridis as determined by x-ray diffraction." Proceedings of the National Academy of Sciences 83, no. 22 (November 1, 1986): 8589–93. http://dx.doi.org/10.1073/pnas.83.22.8589.

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43

Call, Toby P., Tian Carey, Paolo Bombelli, David J. Lea-Smith, Philippa Hooper, Christopher J. Howe, and Felice Torrisi. "Platinum-free, graphene based anodes and air cathodes for single chamber microbial fuel cells." Journal of Materials Chemistry A 5, no. 45 (2017): 23872–86. http://dx.doi.org/10.1039/c7ta06895f.

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In this work graphene-based aerogel anodes and graphene/stainless steel cathodes have been optimised as platinum-free electrodes in Rhodopseudomonas palustris microbial fuel cells, achieving a maximum power output of ∼3.5 W m−3.
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44

Neunlist, S., and M. Rohmer. "A novel hopanoid, 30-(5'-adenosyl)hopane, from the purple non-sulphur bacterium Rhodopseudomonas acidophila, with possible DNA interactions." Biochemical Journal 228, no. 3 (June 15, 1985): 769–71. http://dx.doi.org/10.1042/bj2280769.

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A novel hopanoid, 30-(5'-adenosyl)hopane, was isolated from the purple non-sulphur bacterium Rhodopseudomonas acidophila and identified. The significance of this triterpenoid in terms of bacteriohopanepolyol biosynthesis, membrane reinforcement and possible interactions with nucleic acids is discussed.
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45

P, PANDIYARAJAN, and SANTHANAKRISHANAN S. "PHOTOSYSNTHETIC BACTERIA: ARE THEY PRESENT IN BIOGAS DIGESTERS?" Madras Agricultural Journal 83, Augest (1996): 501–2. http://dx.doi.org/10.29321/maj.10.a01042.

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Attempts were made to isolate photosyntheite bacteria in transluscent biogas digesters made of fibreglass reinforced plastic which allow light to pass through. The results revealed the presence of purple non sulphur bacteria possibly of the genus Rhodopseudomonas in the digester.
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46

Wang, Li Qiu, Xin Xin Deng, and Liang Tian. "A Novel Photosynthetic Bacteria Solar Cell." Advanced Materials Research 773 (September 2013): 97–100. http://dx.doi.org/10.4028/www.scientific.net/amr.773.97.

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In this paper, a novel photosynthetic bacteria solar cell with Rhodopseudomonas as the electricity generation bacteria was designed and prepared, and photo current changing with the time of the cell was investigated by testing current-time(i-t) curve under alternating light and dark. The results showed that the maximum photo current of the cell could be 13 μA. The influence of glucose, sucrose, chitosan, anthraquinone and hydroquinone on the photo current was investigated. The results indicated that glucose and chitosan made the photo current of solar cell increased about 21 μA and 27 μA, respectively; but sucrose, anthraquinone or hydroquinone had not such effect. It illustrated that photo electrons could be emitted by Rhodopseudomonas and were transmitted to the anode of the cell in the presence of electronic media under light, and the photo current could be improved further by the adding of some additives.
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47

Koga, Aoi, Takumi Yamasaki, Shuhei Hayashi, Shinjiro Yamamoto, and Hitoshi Miyasaka. "Isolation of purple nonsulfur bacteria from the digestive tract of ayu (Plecoglossus altivelis)." Bioscience, Biotechnology, and Biochemistry 86, no. 3 (January 10, 2022): 407–12. http://dx.doi.org/10.1093/bbb/zbac001.

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ABSTRACT Purple nonsulfur bacteria (PNSB) reportedly have probiotic effects in fish, but whether they are indigenous in the digestive tract of fish is a question that requires answering. We attempted to isolate PNSB from the digestive tract of ayu (Plecoglossus altivelis) from the Kuma River (Kumamoto, Japan) and successfully isolated 12 PNSB strains. All the isolated PNSB belonged to the genus Rhodopseudomonas. Five Rhodopseudomonas strains were also isolated from the soil samples collected along the Kuma River. The phylogenetic tree based on the partial sequence of pufLM gene indicated that the PNSB from ayu and soil were similar. The effects of NaCl concentration in growth medium on growth were also compared between the PNSB from ayu and soil. The PNSB from ayu showed a better growth performance at a higher NaCl concentration, suggesting that the intestinal tract of ayu, a euryhaline fish, might provide suitable environment for halophilic microorganisms.
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48

Pradebon, Leonardo Cesar, Jaqueline Piesanti Sangiovo, Ivan Ricardo Carvalho, Murilo Vieira Loro, Wilson Story Venâncio, Amanda Maria Nascimento, and Larissa Alves de Castro Jocarelli Rossini. "Inoculants associated with fertilizers: agronomic increases in bean, soybean and lettuce crops." CONTRIBUCIONES A LAS CIENCIAS SOCIALES 17, no. 3 (March 20, 2024): e4417. http://dx.doi.org/10.55905/revconv.17n.3-250.

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The objective of the study was to prove the efficiency of inoculation associated with synthetic fertilizers in the agronomic potential of bean, soybean and lettuce. Experiments were carried out in a randomized block design to evaluate the efficiency of inoculation associated with synthetic fertilizers in bean, soybean and lettuce crops. The experimental design used was randomized blocks organized in a factorial scheme, with six replications. In beans, ten treatments were evaluated in four environments. In soybean cultivation, 13 treatments were evaluated in four environments. While in lettuce, six treatments were evaluated in five environments. In each crop, the agronomic and nutritional characteristics were evaluated. Inoculation associated with fertilizers enhances the agronomic and nutritional performance of beans, soybeans and lettuce. Rhodopseudomonas palustris combined with phosphorus and nitrogen fertilization improves bean grain yield. Bradyrhizobium japonicum, Azospirillum brasiliense and Pseudomonas fluorescensare crucial for soybean. Addition of the bacterium Rhodopseudomonas palustris maximized the lettuce biomass.
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49

Тарабас, О. В., С. O. Гнатуш, A. A. Галушка, and О. M. Mороз. "PIGMENTS OF RHODOPSEUDOMONAS YAVOROVII IMV B-7620." Microbiology&Biotechnology, no. 1(41) (March 29, 2018): 57–65. http://dx.doi.org/10.18524/2307-4663.2018.1(41).120468.

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50

Govindjee, T. S. Desai, V. G. Tatake, and P. V. Sane. "A NEW GLOW PEAK, IN RHODOPSEUDOMONAS SPHAEROIDES." Photochemistry and Photobiology 25, no. 1 (January 2, 2008): 119–22. http://dx.doi.org/10.1111/j.1751-1097.1977.tb07431.x.

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