Dissertations / Theses on the topic 'Rhodopseudomonas'
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Evans, Katie. "Biochemical characterisation of bacteriophytochromes from Rhodopseudomonas palustris." Thesis, Liverpool John Moores University, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.431289.
Miller, Anthony Raymond Miller. "Investigation of Sulfur Salvage Pathways in Rhodopseudomonas palustris." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1511908420156965.
Emery, Vincent Clive. "Mechanistic aspects of bacteriochlorophyll A biosynthesis in Rhodopseudomonas sphaeroides." Thesis, University of Southampton, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259664.
Vuillet, Laurie. "Caractérisation des bactériophytochromes identifiés chez Rhodopseudomonas palustris et bradyrhizobium." Montpellier 2, 2007. http://www.theses.fr/2007MON20207.
Rhodopseudomonas palustris is a photosynthetic purple bacteria which genome was completely sequenced. Six genes encoding putative bactériophytochromes are present in this genome. One of them plays an essential and unusual role in the synthesis of the photosystem. In this bacteria, three other bacteriophytochromes are localized near pucBA operons encoding polypeptides involved in the formation of light harvesting complexes associated with photosystem. First, this PhD work studied roles, properties and mechanisms of action of these three bacteriophytochromes. We showed that RpBphP2 and RpBphP3 act in tandem to regulate the expression of LH4 antennas. This signalling pathway use three reponse-regulators, one of them, Rpa3018 is rdox sensitive. In some strains of Rps. Palustris, a bactériophytochrome, RpBphP4, lost its light sensitivity and acquired a redox sensibility while keeping its ability to regulate the expression of these antennas. In a second time, analysis of the genome sequence of two photosynthetic Bradyrhizobium (ORS278 and BTAi1) revealed, in each strain, the presence of a specific bacteriophytochrome probably acquired by horizontal transfer. The properties of these various bacteriophytochromes led highlighted the variety of this family of light sensors as well as the complexity of the signalling pathways which they introduce
Kamal, Varsha Subhash Carleton University Dissertation Biology. "The anaerobic, phototrophic metabolism of 3-chlorobenzoate by Rhodopseudomonas Palustris." Ottawa, 1992.
Laing, Ruth Mary Louise. "Development of Rhodopseudomonas palustris as a chassis for biotechnological applications." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/283194.
Pott, Robert William McClelland. "The bioconversion of waste glycerol into hydrogen by Rhodopseudomonas palustris." Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708006.
Meckenstock, Udo Rainer. "Isolierung, Charakterisierung und Strukturanalyse des lichtsammelnden B880-Antennenkomplexes von Rhodopseudomonas marina /." [S.l.] : [s.n.], 1993. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10404.
Salmon, Robert. "The transport and degradation of lignin-derived aromatic compounds by Rhodopseudomonas palustris." Thesis, University of Sheffield, 2014. http://etheses.whiterose.ac.uk/7701/.
Bernstein, Jeffrey Robert. "Horizontal pathway transfer to Escherichia coli from Rhodopseudomonas palustris transcription, translation, pathway extension /." Diss., Restricted to subscribing institutions, 2007. http://proquest.umi.com/pqdweb?did=1495958891&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.
Bird, Lina J. (Lina Joana). "Interactions of Fe(II) with the iron oxidizing bacterium Rhodopseudomonas palustris TIE-1." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/81030.
"June 2013." Cataloged from PDF version of thesis.
Includes bibliographical references.
Microbial anaerobic iron oxidation has long been of interest to biologists and geologists, both as a possible mechanism for the creation of banded iron formations before the rise of oxygen, and as a model system for organisms able to accept electrons from an external, inorganic source. Previous work with the purple photoferrotroph Rhodopseudomonas palustris TIE-1 showed that three genes were required for phototrophic growth with Fe(Il): PioA, a decaheme cytochrome, PioB, an outer membrane porin, and PioC, a high potential iron protein (HiPIP). These proteins suggested a model of Fe(II) oxidation that ends with transfer of electrons to the photosynthetic reaction center. The goal of this thesis was to test and extend this model through characterization of the electron transfer proteins PioA and PioC. In the course of our experiments, we discovered that Fe(II) could also delay growth under certain conditions. We then broadened our focus to encompass several facets of the interaction of TIE-1 with Fe(II) under anaerobic conditions: The first portion describes how low amounts of Fe(II) cause a growth delay in TIE-1 cultures growing anaerobically on other substrates - a surprising result for an organism that grows on millimolar concentrations of iron. The cause of this toxicity was found to be dependent on copper, which is toxic to TIE-i at fairly low concentrations. Our results indicate the copper toxicity is synergistically increased by Fe(II) under strictly anaerobic conditions. The second part of this work describes characterization of the HiPIP PioC and a second HiPIP in the TIE-1 genome. The results showed that PioC is capable of reducing the reaction center, as expected, though at a slower rate than is usually found for this kind of interaction. The second HiPIP cannot reduce the reaction center and likely serves an alternate function in the cell unrelated to photosynthesis, possibly involving detoxification of metals. The final section redefines our understanding of the Fe(II) oxidation pathway by putting it in the context of reverse electron transfer, a process that is not well understood in photosynthetic bacteria. Evidence from whole cell experiments using flash induced spectrometry indicated that electrons from Fe(II) may, rather than going to the reaction center, enter the quinone pool through the bc1 complex. This model is significantly different from previous preferred models of phototrophic oxidation, but is similar to the reverse electron transfer system described in acidophilic lithotrophic iron oxidizing bacteria. Taken together, the experiments described in this thesis highlight the complex and interconnected nature of a bacterial cell's interactions with iron under anoxic conditions. It also suggests future avenues of study for phototrophic reverse electron transfer, a poorly understood process that is vital to anoxygenic photoautotrophic growth.
by Lina J. Bird.
Ph.D.
Sebban, Pierre. "Migration et stabilisation de l'énergie d'excitation lumineuse chez la bactérie pourpre Rhodopseudomonas sphaeroides." Paris 11, 1985. http://www.theses.fr/1985PA112088.
The harvesting of light energy is one of the most important function of the photosynthetic organisms. Lt is performed by the chlorophyll antenna molecules. The absorbed energy is transferred very efficiently to the reaction centers where a transmembrane charge separation takes place, converting the energy of photons to chemical free energy. These processes have been studied on the photosynthetic purple bacteria Rhodopseudomonas sphaeroides, by analyzing the time-resolved fluorescence data of the antenna emission. Data obtained on isolated antenna-complex, on mutant lacking one type of antenna and on isolated reaction canters depleted of antenna, were compared to the data obtained on intact systems. Our results are leading to conclusions about the energy migration mechanism in the antenna of purple bacteria (chapter IV), about the antennae to reaction center relationship (chapter II), and about the origin of variable fluorescence (chapter I and III) in relation to the stabilization of the charge separation state of the reaction state
Akbar, Abrar. "Anaerobic degradation of long chain phenylalkane carboxylates by the phototrophic bacterium Rhodopseudomonas palustris." Thesis, University of Sheffield, 2016. http://etheses.whiterose.ac.uk/14272/.
Perry, Leslie M. "Regulation of Alternative Sigma Factors During Oxidative and Ph Stresses in the Phototroph Rhodopseudomonas Palustris." Thesis, University of North Texas, 2014. https://digital.library.unt.edu/ark:/67531/metadc700009/.
Gorham, Hazel Claire. "Characterisation of a novel bacteriophage, 0BHG1, and its interactions with its host Rhodopseudomonas blastica." Thesis, University of Warwick, 1987. http://wrap.warwick.ac.uk/110014/.
Delachapelle, Sylvie. "Dégradation en réacteur pilote du lactate avec photoproduction d'hydrogène par une bactérie photosynthétique : Rhodopseudomonas capsulata." Grenoble INPG, 1987. http://www.theses.fr/1987INPG0011.
Joshi, Gauri Suresh. "Regulation of CO2 fixation in Rhodopseudomonas palustris mediated by a unique two-component regulatory system." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1273605616.
Bacusmo, Jo Marie. "Proline Codon Translational Fidelity in Rhodopseudomonas palustris: Characterization of Novel Trans-editing Factor ProXp-abu." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1397747141.
Untereiner, Guillaume. "Etude de l'impact de bacteries environnementales sur la speciation de l'uranium en vue de processus de bioremediation." Phd thesis, AgroParisTech, 2008. http://pastel.archives-ouvertes.fr/pastel-00004694.
Farsi, Reem. "The role of nitroreductases, nitrilases and nitrile hydratase in breakdown of aromatic compounds in Rhodopseudomonas palustris." Thesis, University of Sheffield, 2017. http://etheses.whiterose.ac.uk/17522/.
Allibert, Patrice. "Clonage et caractérisation d'un gène de Escherichia coli complémentant une mutation de type ntr chez Rhodopseudomonas capsulata." Grenoble 2 : ANRT, 1986. http://catalogue.bnf.fr/ark:/12148/cb37595503p.
Xiao, Ning. "Use of a purple non-sulphur bacterium, Rhodopseudomonas palustris, as a biocatalyst for hydrogen production from glycerol." Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/269754.
Allibert, Patrice. "Clonage et caractérisation d'un gène de Escherichia coli complémentant une mutation de type ntr chez Rhodopseudomonas capsulata." Grenoble 1, 1986. http://www.theses.fr/1986GRE10079.
Kuzmishin, Nagy Alexandra Burden. "Maintaining Fidelity of Translation by Bacterial Trans-Editing Proteins:Caulobacter crescentus ProXp-ala and Rhodopseudomonas palustris ProXp-x." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1563478757446243.
Brotosudarmo, Tatas Hardo Panintingjati. "Studies on the different types of LH2 complexes from the purple non-sulphur photosynthetic bacterium Rhodopseudomonas palustris strain 2.1.6." Thesis, University of Glasgow, 2009. http://theses.gla.ac.uk/1619/.
Pankan, Aazraa Oumayyah. "Performance analysis of bioanode materials and the study of the metabolic activity of Rhodopseudomonas palustris in photo-bioelectrochemical systems." Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/288482.
Kojadinovic, Mila. "Effets antagonistes de l'oxygène et de la lumière sur la régulation de la photosynthèse et de la respiration chez Rhodopseudomonas palustris." Aix-Marseille 2, 2007. http://theses.univ-amu.fr.lama.univ-amu.fr/2007AIX22041.pdf.
The photosynthetic purple bacterium Rhodopseudomonas palustris is able to develop using photosynthesis or respiration. Extraordinarily, it possesses six bacteriophytochromes and two PpsR transcriptional regulators. This work aimed at studying bacteriophytochromes and PpsR roles in the regulation of photosynthesis and respiration by light and oxygen. The study of photosystem synthesis and respiratory activity in Rps. Palustris WT, or inactivated in bacteriophytochromes or in PpsRs showed that (i) the bacteriophytochrome RpBphP1, activated by a far red illumination can trigger photosystem synthesis, by counterbalancing PpsR2 repressive effect, from microaerobic to aerobic conditions ; (ii) that LH2 antennae synthesis is activated by the PpsR1 transcriptional regulator and the bacteriophytochrome RpBphP4, which, depending on the Rps. Palustris strain, can be an oxygen sensor or a light sensor and (iii) that RpBphP1, PpsR1 and PpsR2 are involved in the regulation of Rps. Palustris respiratory activity by far red light. Considering the results obtained combining in silico, molecular biology and biochemistry approaches, we propose that the bacteriophytochrome RpBphP1, by inhibiting PpsR2 activation on the transcription of sucA – encoding the subunit E1 of the alpha-ketoglutarate dehydrogenase, a central enzyme in the Krebs cycle – is responsible for a 40% respiration limitation under far red illumination relative to darkness. Our results showed that a far red illumination, via RpBphP1 action on PpsR2, has a double effect since it activates photosystem synthesis on the one hand, and limits respiration on the other hand. This work also revealed that PpsR1 and PpsR2 regulators are both transcriptional repressors and activators, which can have joint or opposite effects. Finally, we showed that the molecular mechanisms responsible for the metabolic regulations encountered in Rps. Palustris are not all retrieved in the phylogenetically closely related bacterium, Bradyrhizobium ORS278
Garcia, Daniel. "Le cytochrome tétrahémique du centre réactionnel de Rhodopseudomonas viridis, sulfoviridis et de Roseobacter denitrificans : structure, fonction et couplage avec le cytochrome BC1." Aix-Marseille 2, 1994. http://www.theses.fr/1994AIX22062.
Johnson, Ethan Thoreau. "Electrostatic interactions and exciton coupling in photosynthetic light-harvesting complexes and reaction centers /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/9196.
Vuillet, Laurie. "Caractérisation des bactériophytochromes identifiés chez Rhodopseudomas palustris et Bradyrhizobium." Phd thesis, Université Montpellier II - Sciences et Techniques du Languedoc, 2007. http://tel.archives-ouvertes.fr/tel-00258550.
Magnin, Jean-Pierre. "Isolement d'une souche Hfr de la bactérie photosynthétique Rhodobacter capsulatus et cartographie du chromosome." Grenoble 1, 1987. http://www.theses.fr/1987GRE10151.
Delachapelle, Sylvie. "Dégradation en réacteur pilote du lactate avec photoreproduction d'hydrogène par une bactérie photosynthétique, Rhodopseudomonae capsulata." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37604346g.
Yi, Tsun-Hsuan, and 尹存瑄. "Phosphoproteome of Rhodopseudomonas palustris." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/66998964193733846884.
臺灣大學
分子與細胞生物學研究所
98
Rhodopseudomonas palustris (R. palustris) is a purple nonsulfur anoxygenic phototrophic bacterium that belongs to the alpha-proteobacteria. It is a common soil and water bacterium that makes its living by converting sunlight to cellular energy and by absorbing atmospheric carbon dioxide and converting to biomass. This microbe can also degrade and recycle components of the woody tissues of plants. Because of its intimate involvement in carbon management and recycling, R. palustris was selected by the DOE Carbon Management Program for genome sequencing. R. palustris exhibits the ability to grow under photoheterotrophic, photoautotrophic, chemoheterotrophic and chemoautotrophic conditions, and can switch between the four different modes of metabolism for survival by protein phosphorylation. Here, we analyzed the phosphoproteome of R. palustris in photoheterotrophic condition with a shotgun approach and identified 80 phosphopeptides from 73 phosphoproteins and 74 phosphopeptides from 68 phosphoproteins at chemohetrotrophic and photoheterotrophic condition, respectively. Our results revealed that the threonine phosphorylated peptide “GGMpTSHAAVVAR” from pyruvate phosphate dikinase (PPDK, RPA1051) in photoheterotrophic condition is elevated more than 2 folds than in chemoheterotrophic condition. PPDK performs a light-dependent activity and plays an important role in carbon fixation in C4 and CAM plants. However, the function of phosphorylated PPDK is still unknown in prokaryotic cell. Here, we showed that PPDK enzyme activity is higher in photoheterotrophic than in chemoheterotrophic. In our in vitro point mutation experiment revealed that threonine phosphorylation site played an important role in regulating PPDK activity. We suggested that light could stimulate threonine phosphorylation of PPDK and might enhance its activity which could regulate the switch mechanism of R. palustris in photoheterotrophic and chemoheterotrophic conditions.
Kundu, Balaram. "Biochemical and bioenergetic aspects of denitrification in `Rhodopseudomonas sphaeroides` forma sp. `denitrificans`." 1986. http://web4.library.adelaide.edu.au/theses/09PH/09phk968.pdf.
Dohse, Barbara. "Charakterisierung von spezifischen Mutanten des Reaktionszentrums von Rhodopseudomonas viridis /." 1995. http://www.gbv.de/dms/bs/toc/321617916.pdf.
Chen, Ting-Yuan, and 陳定遠. "Systems Analysis of Rhodopseudomonas palustris in Different Metabolic States." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/95163986877750272576.
國立臺灣大學
分子與細胞生物學研究所
96
Rhodopseudomonas palustris (R. palustris), a purple nonsulfur bacteria, is among the most metabolically versatile bacteria and ubiquitous in soil and water. The microbe could use sunlight, organic or inorganic compound for cellular energy, and use atmosphere carbon dioxide, or green plant-derived compound for cell material. It also could produce hydrogen gas by biological nitrogen fixation. The microbe could adjust and reweave itself in response to changes in light, carbon, nitrogen and electron sources. The ability of R. palustris to use carbon dioxide depends on two metabolic states: photoautotrophic and chemoautotrophic state. In this study, we used microarray for transcriptomic study, and two-dimensional polyacrylamide gel electrophoresis and mass spectrometry for proteomic study, respectively. Our results showed that 1357, 2512, 1629, 168 genes were differentially expressed over two-fold in photoautotrophic verus chemoautotrophic conditions, chemoautotrophic versus chemoheterotrophic conditions, photoautotrophic versus photoheterotrophic conditions, and chemoheterotrophic versus photoheterotrophic conditions, respectively. We identified 38 proteins differentially expressed over two-fold between two of the four major metabolic states. Some proteins, such as 60kDa chaperonin 2, ABC transporter related proteins, phosphoenolpyruvate carboxykinase, catalase, and phosphoglycerate dehydrogenase, could differentially expressed in three of these four major metabolic states. Interestingly, ABC transporter pathway was related to switch of autotroph and heterotroph. With computational prediction of plausible protein-protein interactions among these 38 proteins, we found 60kDa chaperonin 2, transcription terminator rho, acyl-CoA dehydrogenase, S-adenosylmethionine synthetase, alcohol dehydrogenase were the hub proteins in the predicted interaction network. These findings help us understand the regulatory mechanism of R. palustris.
Shih, Sniuan-You, and 施宣佑. "The Effect of Rhodopseudomonas sphaeroides to Nitrogen Source in Aquatic System." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/79789768276885596202.
中華醫事科技大學
生物科技研究所
97
The nitrogen cycle of aquatic ecosystem plays an important role, especially inaquatic farms. The effluent of nitrogen source is an invisible killer that could harm the aqua products. In the seawater or fresh water systems, the concentration of Ammonium(NH4+) and Nitrous acid(NO2-) higher than 2mg/L may cause the pathological change of fish. Therefore,this research was designed to study the use of Photosynthetic bacteria (PSB) to biomanipulate the concentration of inorganic nitrogen in the aquatic system. It might be helpful to the aquatic farms and aquatic environments if the increase of nitrogen concentrate is controlled efficiently. Rhodopseudomonas sphaeroides was used in this study and Microbial Pesticides methods were applied to control the content of nitrogen source in aquatic experiments. The results show that the amount of bacteria was saturated after 120 hours culture of R. sphaeroides. The saturated bacterial concentration reached to 109no./ml. In the process of culture, the pH value becomes weak alkalescence after 24 hour culture. The correlation between the amount of R.sphaeroides and the absorbance value of optical density at 660 nm is R2=0.9941. The Abs(OD660) values were utilized to measure the quantities of R. sphaeroides(Klaas,1982). Then, R. sphaeroides (108 no./ml) were added in five different concentrations of Nitrous acid in water to study the removal of Nitrous acid after 24 hours. The results show that all Nitrogen acid can be removed by R. sphaeroides completely. Next, R. sphaeroides (108 no./ml) were added in five different concentrations of Ammonium to study the removal rate of Ammonium. The removal rate of Ammonium is about 58% after 120 hours. Third, among three levels of bacterium concentration, the concentration of 109no. /ml is the best for removing Nitrous acid. Fourth, R. sphaeroides can stabilize the pH value in the water of aquaculture pond, but it has no effect on the removal of Nitrogen.
Sheng-Yu, Lin, and 林陞昱. "The interactions in co-culture of Rhodopseudomonas palustris and Bacillus megaterium." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/46026516339357919338.
中國文化大學
生物科技研究所
88
Purple nonsulfur bacteria have been known to have potential use in agriculture and environment protection. They have been reported to enhance the soil fertility, stimulate flower bud diffraction and fruit maturation, and improve wastewater treatment and malodor removing and produce of single cell protein and amino acid for animal feed. In this study we screen and isolated purple nonsulfur bacteria from waste of local hog farm and investigate the results of co-culture with Bacillus megaterinm for the purple nonsulfur bacteria. In these studies the experiment on growth rate of purple nonsulfur bacteria are conducted with various cultural and nutrient conditions. The reduction of acetylene to ethylene is used to estimate the activity of nitrogen fixation. Comparison of the amount of ethylene production is conducted before and after the co-culture experiments for the investigation of our nitrogen fixation experiment. Our results show that the purple nonsulfur bacteria from local manure identify to be Rhodopseudomonas palustris and can grow under both aerobic and anaerobic condition with light and without light illumination for more than ten months. Cultures of Rhodopseudomonas palustris are red in anaerobically grown condition and yellowish white in aerobically grown condition. Under the 3000 lux illumination the generation time of Rhodopseudomonas palustris shorten and the pigment of bacteriochlorophyll a of each cell lessen as light intensity increase. The activity of nitrogen fixation of Rhodopseudomonas palustris and Bacillus megaterium are insignificant, however a 10-15 times increase while both bacteria culture together. The activity of nitrogen fixation of Rhodopseudomonas palustris demonstrates to be enhanced by co-culture with Bacillus megaterium.
Chang, Yaling-Ling, and 張雅菱. "Effects of Overexpressed Transketolase Genes on Carbon Metabolism in Rhodopseudomonas palustris." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/45783893182340647406.
國立臺灣大學
分子與細胞生物學研究所
97
Transketolase is a thiamin diphosphate (ThDP)-dependent enzyme that functions as a key catalyst in the oxidative pentose phosphate pathway of virtually all organisms. It also plays an important role in the Calvin-Benson-Bassham (CBB) reductive pentose phosphate cycle of photosynthetic organisms. In Rhodopseudomonas palustris, a purple, non-sulfur photobacterium, there are two isoforms of transketolase, CbbT1 and CbbT2, encoded by transketolase genes, cbbT1 (rpa4643) and cbbT2 (rpa0945), respectively. In this study, we overexpressed cbbT1 and cbbT2 under photoautotrophic conditions, in which CO2 was the only carbon source. We integrated oligo microarray analysis, real-time quantitative PCR (qPCR), bioluminescent ATP assay and absorbance spectra to study the effects of overexpressed transketolase. From our microarray data, overexpressed cbbT1 had more effects on R. palustris than overexpressed cbbT2. In the T1 strain, an increase of the peak amplitudes in the absorption spectrum and increased expression of related photosynthetic genes were observed. On the other hand, we measured the glycolytic ATP synthetic activity by bioluminescent ATP assay and observed an increased amount of ATP in the T2 strain. Moreover, immunogold labeling of HA-tag fusion tranketolase reveals that CbbT1 is mainly located in the intracytoplasmic membrane systems, but CbbT2 in the cytosol and the ICM structures are not abundant in the T2 strain compared with the T1 strain. In this study, we suggest that the two isoforms of transketolase are likely involved in different carbon metabolic pathways and have distinct effects on bacterial physiology.
Fejes, Anthony Peter. "Mass spectrometry of Rhodopseudomonas palustris chromatophores and a method for displaying proteomes." Thesis, 2004. http://hdl.handle.net/2429/15183.
Kuo, Fu-Shiu, and 郭甫旭. "Effects of Light Sources on Growth and Carotenoids Content of Photosynthetic Bacteria Rhodopseudomonas palustris." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/39696528163929525870.
國立臺灣海洋大學
水產養殖學系
97
The influence of different light sources on the growth and carotenoid content of photosynthetic bacteria (Rhodopseudomonas palustris) was studied. For the experiment set up, 27 250ml flasks were used and 25ml (4.4×104 cell/ml ) photosynthetic bacteria solution per flask was added. Light intensity was set to 2000±100 lux. Eight light sources were used: incandescent lamp (IL), halogen lamp (HL), fluorescence lamp (FL), light-emitting diode (LED): LED white (LW), LED yellow (LY), LED red (LR), LED blue (LB), LED green (LG). Dark (DK) served as control. Each of the nine treatments had triplicates. Two trials were conducted to culture the bacteria for 96 and 144 hours. In the first trial, the effect of light sources on bacteria growth was: LB>IL>HL>FL≧(LW=DK=LG=LR)≧LY and on carotenoid content: LB>IL≧LY≧(HL=LR=LG) ≧LW≧DK≧FL. In the second trial, light resource effect on growth was: (LB=IL)>FL>LW≧HL≧LR≧(LG=LY=DK). Bacteria biomass production per unit electric power consumption were: LB>LW>LY>IL>LG>HL>FL>LR. Production of photosynthetic bacteria by LB can save up to 75% of electricity cost than by IL. This study provides practical application of illumination for the production of photosynthetic bacteria.
Shen, Meng-Wei, and 沈孟薇. "Effects of Rhodopseudomonas palustris PS3 on nitrogen and carbon metabolism in the Brassica rapa L. ssp. chinensis." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/21971629057941334134.
國立臺灣大學
生化科技學系
104
Rhodopseudomonas palustris NTUIOB-PS3, a phototrophic purple non-sulfur bacterium, was originally isolated from paddy field in Taiwan. It can not only improve plant growth, but also reduce the nitrate accumulation in plants. The aim of this study was to investigate the mechanism of its plant growth promotion effect. We would like to elucidate the effects of rhizosphere bacteria on plant physiology through evaluating the nitrogen uptake efficiency, activity of nitrogen assimilation associative enzymes, gene expression of nitrate transporters, and CO2 assimilation rate of plants. When hydroponic solution was supplemented with the PS3 inoculant, plant growth (fresh weight of shoots and roots) of Chinese cabbage was greater than that without inoculation (control group). Since the gene expression of the nitrate transporter BjNRT1.1 in the roots and total amounts of N in the plants were dramatically elevated, we deduced that PS3 could stimulate the N uptake efficiency (NupE) of plants. The N uptake efficiency was around 40% increase in comparison with that in the control group. On the other hand, the plants inoculated with PS3 showed higher photosynthetic rate in the early stage of plants, then the leave sizes and total C amounts in shoot were increased nearly 25 % and 70%, respectively. Taken together, we deduced that PS3 improves both N and C metabolic efficiencies of plants through plant-microbe interaction, as a result of higher biomass accumulation.
Sung, Fa-Yi, and 宋法逸. "Try to prolong photo-hydrogen production of Rhodopseudomonas palustris NifAnQ for long-term operation under continuous condition." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/68208752262720161889.
國立中興大學
環境工程學系所
102
Environmental concerns, energy shortage, and consequently increasing energy costs result in the need to produce sustainable and renewable fuels. As Hydrogen has high energy density (142 MJ/Kg), a sustainable platform of hydrogen utilization can be established if it can be generated sustainably and used safely, as well as being stored and transported efficiently. There are various ways to produce hydrogen, among which photosynthetic hydrogen production by purple non-sufur photosynthetic bacteria (PNSB) is one of the favorable methods for biohydrogen production because of its high substrate conversion efficiency and its capability of utilizing abundant substrates. The most serious problem of photohydrogen production is that hydrogen production is inhibited by high ammonium concentration. The hydrogen production center of PNSB, the nitrogenase, is regulated by NifA protein. High ammonium concentration will inhibit NifA protein activities, and hence inhibit the transcription of nitrogenase gene and its expression. As a result, no hydrogen can be produced. The mutant of Rhodopseudomonas palustris WP3-5, Rhodopseudomonas palustris NifAnQ was constructed by molecule biotechnology. This mutant can tolerate high ammonium concentration. In this study, this mutant was employed to treat dark-fermentation effluent as substrate to recover hydrogen energy. The dark-fermentation effluent is rich in organic acid including acetate (1~6 g/L), lactate(1 g/L), butyrate(0.4~0.6 g/L), and high ammonium concentration(135 mg/L). This study treats the effluent as continuous culture condition’s substrate. To stabilize the pH value in the reactor, a continuous monitoring system was set up to prevent non-toxic form from transforming to toxic form of ammonium. At a high level of ammonium concentration with acetate and butyrate being carbon sources, the mutant photobioreactor operated continuously at HRT = 12 hr ; the maximum hydrogen concentration and hydrogen production rate are 74% and 172 mL-H2/day. At a high level of ammonium concentration with lactate being carbon source, it was proven that the wild type photobioreactor could not generate hydrogen. This study proved that mutant photobioreactor can operate under continuous culture condition with high ammonium concentration synthetic wastewater, and it can generate hydrogen continuously over 57 days. This demonstrates that the mutant photobioreactor has the capacity to treat effluent with high ammonium concentration to recover most of hydrogen energy.
Mishra, Shambhavi. "Structural and Functional Characterization of the Enzymes Involved in the Menaquinone Biosynthesis and Benzoate Degradation." Doctoral thesis, 2013. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-90848.
Die vorliegende Arbeit befasst sich mit der strukturellen und biochemischen Charakterisierung der beiden unterschiedlichen bakteriellen Enzyme BadI von Rhodopseudomonas palustris und MenD von Staphylococcus aureus. Die 2-Ketocyclohexancarboxyl-CoA-Hydrolase BadI ist eines der Schlüsselenzyme des anaeroben Abbaus aromatischer Verbindungen. Der Abbau aromatischer Verbindungen ist essentiell für die Aufrechterhaltung des biogeochemischen Kohlenstoffkreislaufs und der biologischen Beseitigung von Xenobiotika, welche in höheren Konzentrationen eine Gefahr für den menschlichen Organismus darstellen können. Wegen des inerten Charakters aromatischer Verbindungen sind Enzyme, welche deren Abbau katalysieren, von besonderem Interesse für industrielle Anwendungen. BadI ist eines der Schlüsselenzyme für den anaeroben Abbau aromatischer Verbindungen zu aliphatischen Gruppen. Das Hauptaugenmerk dieses Projekts lag auf der Aufklärung des Reaktionsmechanismus, welcher von BadI katalysiert wird. BadI gehört zur Überfamilie der Crotonasen und zeigt hohe Sequenzhomologie mit der zugehörigen Dihydroxynaphthoat-Synthase MenB. Durch die Hydrolyse einer C-C Bindung katalysiert BadI den Schnitt des zyklischen Rings von 2-Ketocyclohexancarboxyl-CoA, welcher zur Bildung der aliphatischen Verbindung Pimelyl-CoA führt. MenB, andererseits, katalysiert die Kondensationsreaktion von O-Succinylbenzyl-CoA zu Dihydronaphthoyl-CoA. Ein umfassender Aminosäuresequenzvergleich zwischen BadI und MenB zeigt, dass die Reste des aktiven Zentrums von MenB aus Mycobacterium tuberculosis (mtMenB) in BadI von R. palustris konserviert sind. MenB ist Teil des Menaquinon Biosynthesewegs und ein potentielles Wirkstoffziel gegen M. tuberculosis, da kein humanes Homolog existiert. Wegen der ausgeprägten Homologie zwischen MenB und BadI und der Tatsache, dass bisher keine MenB-Inhibitor Komplex Strukturen gelöst werden konnten, erweiterten wir unser Interesse auf BadI, da es als Model für mtMenB als Wirkstoffziel dienen könnte. Darüber hinaus besitzt BadI einige einzigartige mechanistische Charakteristika. Wie zuvor erwähnt, hydrolysiert es das Substrate durch eine reverse Dieckmanns Reaktion in Gegensatz zu seinem ähnlichsten Homolog MenB, das einen Ringschluss durch eine Dieckmanns Reaktion katalysiert. Dennoch scheinen die Reste des aktiven Zentrums streng konserviert zu sein. Daher entschieden wir die strukturelle Charakterisierung von BadI anzugehen um Gemeinsamkeiten und Unterschiede zwischen BadI und MenB aufzuzeigen und einen Einblick zu erhalten, wie sie die gegenläufigen Reaktionen durchführen. Wir lösten die ersten Strukturen von BadI in seiner Apo-Form und einer Substrat-Mimik gebundenen Form. Die Kristallstrukturen von BadI zeigten die gleiche Gesamtfaltung wie andere Mitglieder der Crotonase Familie. Allerdings gibt es in BadI kein Anzeichen für Domain-Swapping, wie es in MenB beobachtet wurde. Das Fehlen des Domain-Swappings ist bemerkenswert, da die vertauschte C-terminale helikale Domäne in MenB ein Tyrosin enthält, welches essentiell für die Katalyse ist und auch in BadI konserviert vorliegt. Der Vergleich des aktiven Zentrums zeigt, dass der C-Terminus von BadI so auf seinen Kern/Hauptteil faltet, dass das konservierte Tyrosin an der gleichen Stelle positioniert ist wie in MenB und mit dem Liganden interagieren kann. Die Struktur von BadI bestätigt auch die Rolle eines Serin- und eines Aspartatrests für die Ligandenbindung und bekräftigt damit, dass das konservierte aktive Zentrum an der enzymatischen Reaktion teilnimmt. Die Strukturen zeigen auch eine bemerkenswerte Verschiebung des aktiven Aspartats, welches zwei Hauptkonformationen einnimmt. Strukturelle Analysen zeigten auch die Nähe des Serinrests zu einem Wasser- und Chlormolekül, sowie einem Kohlenstoffrest, an dessen Stelle der Carbonylrest des eigentlichen Substrats läge. Die biochemische Charakterisierung von BadI in enzymkinetischen Untersuchungen bestätigte dass die vorgeschlagenen Reste des aktiven Zentrums an der Substratbindung beteiligt sind. Jedoch ist die Rolle der verschiedenen Reste sehr verschieden, wobei dem Serin eine herausragende Rolle zugedacht wird. Die hier dargestellte Arbeit bestätigt die Mitwirkung des mutmaßlichen aktiven Zentrums und zeigt, dass die Reste des Aktiven Zentrums von BadI eine unterschiedliche Rolle, im Vergleich zu ihrem ähnlichsten Homolog MenB, spielen. MenD, eine SEPHCHC (2-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carbonsäure) Synthase, ist an der Menaquinonbiosynthese von S. aureus beteiligt. Obwohl S. aureus gewöhnlich als Kommensale betrachtet wird, kann es als bemerkenswertes Pathogen auftreten, wenn es die Epithelwand durchbricht und eine Vielzahl an Erkrankungen, von einfachen Hautinfektionen bis zu lebensbedrohlichen Zustanden, verursachen. Sogenannte „Small colony variants“ (SCVs), eine langsam wachsende, kleinzellige Subpopulation der Bakterien wurde mit persistenten, rezidivierenden und antibiotika-resistenten Infektionen assoziiert. Diese Varianten weisen einen Mangel von Thiamin, Menaquinon und Hämin auf. Menaquinon ist ein essentieller Bestandteil der Elektronentransport-Kette in grampositiven Organismen. Daher sind Enzyme dieses Stoffwechselwegs attraktive Wirkstoffziele gegen Krankheitserreger wie M. tuberculosis oder Bacillus subtilis. MenD, das Enzym, welches den ersten irreversiblen Schritt des Menaquinon-Biosynthesewegs katalysiert, wurde mit dem SCV Phänotyp von S. aureus in Verbindung gebracht. In dieser Arbeit werden die biochemischen und strukturellen Eigenschaften dieses wichtigen Enzyms untersucht. Unsere strukturelle Untersuchung zeigte, dass trotz einer niedrigen Sequenzidentität von 28%, die Gesamtfaltung von S. aureus MenD (saMenD) mit derjenigen von Escherichia coli MenD (ecMenD), trotz einiger signifikanter Abweichungen, übereinstimmt. Größere strukturelle Unterschiede können nahe des aktives Zentrums des Proteins beobachtet werden, vor allem in der C-terminalen Helix und einer Schleife nahe dem aktiven Zentrum. Die Schleife enthält kritische Reste für die Kofaktorbindung und liegt nur in der ecMenD-ThDP Komplexstruktur definiert vor, während die in der Apo-Form und der Substrat-gebundenen Struktur von ecMenD ungeordnet ist. In unserer saMenD Struktur zeigt sich die Schleife erstmals komplett geordnet in der Apo-Form und stellt eine neue Konformation der Kofaktor-Bindeschleife dar. Die Schleife nimmt eine ungewöhnlich offene Konformation an und die konservierten Reste, welche für die Kofaktorbindung verantwortlich sind, sind zu weit entfernt, um in dieser Position einen produktiven Komplex mit dem Kofaktor zu bilden. Zudem haben biochemische Studien in Verbindung mit den strukturellen Daten zur Identifizierung der Substratbindetasche und der an der Bindung und Katalyse beteiligten Aminosäuren beigetragen. In der vorliegenden Arbeit wurden die biochemischen und strukturellen Charakteristika von saMenD erfolgreich aufgeklärt
Lu, Pi-Fen, and 呂碧芬. "Assessing the feasibility of hydrogen production by co-cultured system combining Rhodopseudomonas palustris WP3-5 and Anabaena sp. CH3." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/24270779076148866009.
國立中興大學
環境工程學系所
97
Nowadays, the aggravation of greenhouse effect by combustion of non-renewable sources of energy such as fossil fuel, coal, oil, and nature gas has become a global issue. However, hydrogen can be an alternative and sustainable energy source due to the properties of clean and high energy yield(122 kJ/g), which is an environmentally friendly technology in the future. Biohydrogen, comparing with the other technologies of hydrogen production, has the advantages of organic waste recycling and cost- effectiveness. Therefore, the aim of this study focuses on the combination of three different types of microorganisms and trying to enhance the efficiency of hydrogen production. This study was divided into two parts. Firstly, after anaerobic fermentation of leftovers, the possibility of using effluent as substrate for phototrophic hydrogen production was evaluated. Secondly, the feasibility of biohydrogen production from co-culture of purple nonsulfur bacterium, Rhodopseudomonas palustris WP3-5, and heterocyst-forming filamentous cyanobacteria, Anabaena sp. CH1, was estimated. In the first part, the inoculum of anaerobic fermentation was isolated from the sludge from wastewater treatment plant via heat treatment and BMP medium enrichment. The results show that the maximum cumulative volume of biohydrogen from semi-continuous anaerobic fermentation system is 248 ml, which is utilizing the leftovers as substrates. The results also indicate that the effluent contains large amount of volatile fatty acid but little amount of ammonium(5 mg/L). Because the ammonium concentration in effluent is lower than the inhibition threshold(17 mg/L)of Rhodopseudomonas plaustris WP3-5 during biohydrogen production, phototrophic biohydrogen system could be successfully compatible with anaerobic fermentation system as two-stage biohydrogen production system. In the second part, the results from co-cultured system show that the maximum cumulative volume of biohydrogen reaches 140.83 ml in the WP3-5/CH1 mixed ratio of 2/1, which increases 2.48 fold comparing with the sum of cumulative volume(56.77 ml)from individual biohydrogen systems. Acetate, the metabolite of fructose via heterotrophic metabolism of Anabaena sp. CH1, could be taken by Rhodopseudomonas plaustris WP3-5 as energy source to produce biohydrogen. Therefore, the results from this study indicate that the co-culture biohydrogen system combining those two phototrophic bacteria is exactly feasible and the efficiency of biohydrogen production by co-cultured system is better than that of the single culture system.
WEI, ZHI-RONG, and 魏志榮. "The effects of photosynthetic bacteria (Rhodopseudomonas capsulatus) on sediment and water under various temperature, salinity and light intensity regime." Thesis, 1990. http://ndltd.ncl.edu.tw/handle/10058397371848666409.
LIANG, RONG-YUAN, and 梁榮元. "The effects of photosynthetic bacteria (Rhodopseudomonas capsulatus) addition and hard clam (Meretrix lousoria) polyculture on kuruma prawn (Penaeus japonicus) culture system." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/28877467851117071216.
Chen, Ying-Tzu, and 陳盈孜. "Relationship between cell growth, hydrogen production and poly-β-hydroxybutyrate (PHB) accumulation by Rhodopseudomonas palustris WP3-5 and co-culture test." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/87779716797277878704.
中興大學
環境工程學系所
99
Hydrogen is a clean energy that generates only water upon burning and it releases high amount of energy (122 kJ/g) by combustion. Photo-biological hydrogen production by purple non-sulfur photosynthetic bacteria is one of promising methods. Purple non-sulfur photosynthetic bacteria can produce hydrogen by nitrogenase under photo-heterotrophic condition. In recent years, many researchers make efforts to improve its efficiency of biological hydrogen production. Poly-β-hydroxybutyrate (PHB) is accumulated as carbon and energy storage material under unbalanced growth. The synthesis of PHB may compete with hydrogen production for energy and reducing power. In addition, maximum hydrogen production rate usually takes place during early stationary phase. Therefore, energy distribution between cell growth, hydrogen production and PHB accumulation is an important issue. Besides, dark fermentation effluent containing various volatile organic acids is suitable for purple non-sulfur photosynthetic bacteria to produce hydrogen. To treat wastewater and recycle clean energy efficiently by biological method are also essential. In this research, Rhodopseudomonas palustris WP3-5 and its PHB synthase-deficient mutant Rps. palustris M23 were used in batch experiments to explore energy distribution between cell growth, hydrogen production and PHB accumulation under different culture conditions. This study also tried to isolate purple non-sulfur photosynthetic bacteria from environment, and tested their ability to produce hydrogen using different volatile organic acids. Then, hydrogen production and treatment efficiency in co-culture system with Rps. palustris WP3-5 were evaluated and continuous photosynthetic hydrogen production was performed. From the results, Rps. palustris WP3-5 accumulated PHB in growth phase, and PHB could be utilized as another carbon and energy source when its content reached maximum value. Microorganism ceased growing when nitrogen source was exhausted, and used excess energy to produce more hydrogen in stationary phase. Rps. palustris WP3-5 could not synthesize PHB under low concentration of carbon source because substrate was degraded rapidly, and there were no difference of hydrogen production between wild-type strain and mutant Rps. palustris M23. When concentration of carbon source was three times higher, cumulated hydrogen volume and substrate conversion efficiency of Rps. palustris WP3-5 were better than Rps. palustris M23. This result coincided with other experiments in different culture conditions. But when using malate as carbon source, Rps. palustris WP3-5 had lower substrate degrading rate and cumulated hydrogen volume, and PHB was not accumulated. Different species of purple non-sulfur photosynthetic bacteria had different substrate assimilation pathway when using same carbon source and accumulated PHB content might be different. Rps. palustris WP3-5 used most energy to produce hydrogen in stationary phase and PHB content was below 10% cell dry weight, accounting for less than 5% of the substrate electrons utilized. This portion of energy might partially redistribute to synthesize soluble microbial product (SMP). Therefore, the competition relationship between hydrogen production and PHB accumulation was insignificant. In second part, several purple non-sulfur photosynthetic bacteria were isolated, and there were no obvious difference in utilizing volatile organic acids to produce hydrogen between these strains. Hydrogen production was not improved and competition for energy might exist in co-culture system. Additionally, new designed photo-bioreactor was used for Rps. palustris WP3-5 to produce hydrogen continuously when using tungsten filament lamp as light source. Operating hydraulic retention time from 2 day to 3 day, gas production rate was highest in initial period, up to 317.6 mL gas/L culture-day. MLSS was always between 220 and 360 mg/L, and MLSS wasn’t enhanced by changing operational strategies. Thinking of light transmission efficiency and problem of light shielding effect caused by high biomass concentration, experimental results was not as expected, and this photo-bioreactor might not favorable for continuous hydrogen production by purple non-sulfur photosynthetic bacteria.
Cheng, Wei-Fang, and 鄭偉凡. "Effects of Light Sources on Growth and Carotenoids Content and Composition of the Photosynthetic Bacteria Rhodopseudomonas palustris, Rhodobacter sphaeroides, Rhodobacter capsulatus." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/26427732956755987728.
國立臺灣海洋大學
水產養殖學系
100
Photosynthetic bacteria have a wide application, is rich in carotenoids, and can be used as probiotics in aquaculture. Since light source can affect the growth of photosynthetic bacteria and their carotenoid composition, this study used 8 light sources: light-emitting diode (LED) green (LG), blue (LB), red (LR), white (LW), yellow (LY), halogen light (HL), incandescent light (IL), and fluorescence (FL) to find out their effects on the growth and carotenoid content of 3 photosynthetic bacteria (Rhodobacter capsulatus, Rhodopseudomonas palustris, Rhodobacter sphaeroides). Three experiments were conducted: Experiment I and II were after low (4.1 x 105 cell/ml) and high (4.2 x 106 cell/ml) concentration of inoculation of 3 photosynthetic bacteria, respectively, the effects of various light sources on growth of photosynthetic bacteria. Experiment III was the effects of various light sources on the carotenoid content of 3 photosynthetic bacteria. Experiment I indicated that for the bacteria growth the sequences were: (LB=IL)>(LW=HL=FL)>LR>(LG=LY)>DK, for the total carotenoid content the sequences were: LB>IL>(LW=HL=FL)>(LG=LR=LY)>DK, and for the bacteria growth and total carotenoid content were: R. capsulatus>R. palustris>R. sphaeroides. Since R. capsulatus had the highest wave length absorbance in 400~500 nm and the wave length of LB was 460~470 nm, the culture of R. capsulatus under LB resulted in the highest production. Experiment II indicated that for the bacteria growth the sequences were: (LB=IL)>FL>LW>(LG=LR)>LY>DK. Cultured under LB the highest bacteria growth was 1.0 x 107cell/ml and 0.9 x 107cell/ml for Experiment I and II, respectively. Although headed with higher inoculation concentration, Experiment II had lower growth, which was attributed to nutrient deficiency and self-shadow effect. In Experiment III, light source effects on the production of carotenoid were as the following: β-carotene: LB>IL>LW>(HL=FL)>(LG=LR=LY)>DK, zeaxanthin: LB>LW>(LY=IL=FL)>(HL=LR=DK=LG), lutein: (LB=LW)>LR>LG>(LY=DK=HL=IL=FL), and canthaxanthin: IL>(LY=HL=FL)>DK>(LG=LB=LR=LY). LB was in the best absorbance range of β-carotene, zeaxanthin and lutein, but not for canthaxanthin.
Hu, Chia-Wei, and 胡家瑋. "Phosphoproteomic studies:(1) Regulation of different metabolic states in Rhodopseudomonas palustris (2) Response pathways of ATP synthase inhibitor in lung cancer cells." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/y8m5d6.
國立臺灣大學
分子與細胞生物學研究所
101
Protein phosphorylation is one of the most important post-translational modifications in cells, including prokaryotes and eukaryotes. Systematic study of phosphoproteome in cells is becoming a crucial determinant for understanding the regulation of cell physiology. In the study, we applied the phosphoproteomic analyses to investigate the molecular regulation in both prokaryotic and eukaryotic systems. An efficient approach, hydroxy acid-modified metal oxide chromatography (HAMMOC), was used for phosphopeptide enrichment. In the prokaryotic phosphoproteome study, the regulation of different metabolic states in Rhodopseudomonas palustris (R. palustris) was studied. R. palustris is a purple nonsulfur anoxygenic phototrophic bacterium with metabolic versatility and is able to grow under photoheterotrophic and chemoheterotrophic states. It also has the potential for bioenergy production and biodegradation. This study is the first to identify the phosphoproteome of R. palustris including 100 phosphopeptides from 54 phosphoproteins and 74 phosphopeptides from 42 phosphoproteins in chemoheterotrophic and photoheterotrophic growth conditions, respectively. In the identified phosphoproteome, phosphorylation at the threonine residue, Thr487, of pyruvate phosphate dikinase (PPDK, RPA1051) was found to participate in the regulation of carbon metabolism. Here, we show that PPDK enzyme activity is higher in photoheterotrophic growth, with Thr487 phosphorylation as a possible mediator. Under the same photoheterotrophic conditions, R. palustris with overexpressed wild-type PPDK showed an enhanced accumulation of total lipids than those with mutant PPDK (T487V) form. This study reveals the role of the PPDK in the production of biodiesel material, lipid content, with threonyl-phosphorylation as one of the possible regulatory events during photoheterotrophic growth in R. palustris. In the eukaryotic phosphoproteomic study, the response pathways of ATP synthase inhibitor in lung cancer cells were investigated. ATP synthase is a multimeric protein complex that catalyzes the synthesis of ATP. For a long time, animal ATP synthase was believed to be found only in mitochondria; however, in recent studies, ATP synthase was also found on the extracellular surface of some cell types including cancer cells, named as ectopic ATP synthase. ATP synthase inhibitor is a potential drug candidate for fighting cancer by blocking the ectopic ATP synthase without obvious damages to mitochondrial function. In this study, we performed quantitative phosphoproteomics to elucidate the molecular response to ectopic ATP synthase inhibition in both cell and xenograft systems. A total of 2834 phosphopeptides covering 836 phosphoproteins and 862 phosphopeptides containing 423 phosphoproteins were identified in cells and xenograft phosphoproteome, respectively. The gene ontology analysis showed that ATP synthase inhibitor treatment had the impacts on phosphorylated proteins involved in protein folding, cell cycle, and cytoskeleton. Clustering and network analysis of time-dependent phosphorylation profiles further revealed that inhibiting ectopic ATP synthase could immediately down-regulate the phosphorylation of heat shock protein 90 (HSP90AB1), and reduce the function of mitogen-activated protein kinase 1 (MAPK1) in the late-response by dual de-phosphorylation of a TEY activation motif, which is involved in the MAPKs/ERKs cascade associated with cancer progression. We next found that the dephosphorylated HSP90-S255A with alanine substitution of the identified phosphorylated residue Ser255 was unable to mediate the phosphorylation of MAPK1 and the activity of MAPKs/ERKs signaling. These results imply that inhibiting ecto-ATP synthase might suppress the cancer growth through the de-phosphorylation of HSP90-regulated MAPKs/ERKs signaling. This study provides the new insight into the development of novel therapeutic strategies that exploit response pathways on lung tumor suppression.