Relevant bibliographies by topics / Rhbmp2

Academic literature on the topic 'Rhbmp2'

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Published: 8 July 2023

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Journal articles on the topic "Rhbmp2"

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Colange, Ana Lucia, Carlos Sabino de Oliveira, Benedito Domingos Neto, Heloisa Sobreiro Selistre de Araújo, Eliane Trovatti, and Monica Rosas da Costa Iemma. "Effect on viability and cellular proliferation of rhBMP-2 immobilized on TEMPO modified cellulose hydrogel." Research, Society and Development 11, no. 11 (August 29, 2022): e471111133260. http://dx.doi.org/10.33448/rsd-v11i11.33260.

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BMP´s are signaling proteins that belong to the Transforming Growth Factor-β (TGF-β) superfamily. These proteins promote the recruitment and differentiation of mesenchymal progenitor cells into bone forming cells, the osteoblasts and increase the rate of bone formation. The carrier systems to release rhBMP-2 to the action site are based on the use of free and soluble BMP incorporated into biopolymers such as collagen, gelatin, chitosan, hyaluronic acid and silk. The fused rhBMP-2-thioredoxin could be an interesting approach for new advances in the field of carrying systems of these growth factors. The fused protein thioredoxin can be useful as a coupling agent of BMP-2 to the carrier system, binding it to the surface of the matrix and it is one of the main aims of this work. The recombinant protein rhBMP-2 was produced by IPTG induction obtaining a soluble protein without the need for refolding process. The immobilization of rhBMP-2 at the surface of the TEMPO modified cellulose nanofibrils was indicated by FTIR spectroscopy. The cellular viability tests indicated increased proliferative behavior of both, C2C12 and stem cells from rats, when seeded in presence of rhBMP2 when compared to the free rhBMP2 substrate. The calcified extracellular matrix confirmed the increased activity of the rhBMP2-cellulose substrate, indicating the success of the proposed method. The cell proliferation assays indicated the method used to immobilize rhBMP2 onto the surface of the TEMPO modified cellulose was successful. The cells growth increased when compared to the reference sample free of rhBMP2.
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Jeong, Byung-Chul, Hyuck Choi, Sung-Woong Hur, Jung-Woo Kim, Sin-Hye Oh, Hyun-Seung Kim, Soo-Chang Song, Keun-Bae Lee, Kwang-Bum Park, and Jeong-Tae Koh. "Repair of Cranial Bone Defects Using rhBMP2 and Submicron Particle of Biphasic Calcium Phosphate Ceramics with Through-Hole." BioMed Research International 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/926291.

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Recently a submicron particle of biphasic calcium phosphate ceramic (BCP) with through-hole (donut-shaped BCP (d-BCP)) was developed for improving the osteoconductivity. This study was performed to examine the usefulness of d-BCP for the delivery of osteoinductive rhBMP2 and the effectiveness on cranial bone regeneration. The d-BCP was soaked in rhBMP2 solution and then freeze-dried. Scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), and Raman spectroscopy analyses confirmed that rhBMP2 was well delivered onto the d-BCP surface and the through-hole. The bioactivity of the rhBMP2/d-BCP composite was validated in MC3T3-E1 cells as anin vitromodel and in critical-sized cranial defects in C57BL/6 mice. When freeze-dried d-BCPs with rhBMP2 were placed in transwell inserts and suspended above MC3T3-E1, alkaline phosphatase activity and osteoblast-specific gene expression were increased compared to non-rhBMP2-containing d-BCPs. For evaluatingin vivoeffectiveness, freeze-dried d-BCPs with or without rhBMP2 were implanted into critical-sized cranial defects. Microcomputed tomography and histologic analysis showed that rhBMP2-containing d-BCPs significantly enhanced cranial bone regeneration compared to non-rhBMP2-containing control. These results suggest that a combination of d-BCP and rhBMP2 can accelerate bone regeneration, and this could be used to develop therapeutic strategies in hard tissue healing.
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Alobaidaan, Raed, Jeremiah R. Cohen, Elizabeth L. Lord, Zorica Buser, S. Tim Yoon, Jim A. Youssef, Jong-Beom Park, Darrel S. Brodke, Jeffrey C. Wang, and Hans-Joerg Meisel. "Complication Rates in Posterior Lumbar Interbody Fusion (PLIF) Surgery With Human Bone Morphogenetic Protein 2: Medicare Population." Global Spine Journal 7, no. 8 (May 16, 2017): 770–73. http://dx.doi.org/10.1177/2192568217696695.

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Study Design: Retrospective cohort study among Medicare beneficiaries who underwent posterior lumbar interbody fusion (PLIF) surgery. Objective: To identify the complication rates associated with the use of bone morphogenetic protein 2 (BMP2) in PLIF. Human BMP2 is commonly used in the “off-label” manner for various types of spine fusion procedures, including PLIF. However, recent studies have reported potential complications associated with the recombinant human BMP2 (rhBMP2) use in the posterior approach. Methods: Medicare records within the PearlDiver database were queried for patients undergoing PLIF procedure with and without rhBMP2 between 2005 and 2010. We evaluated complications within 1 year postoperatively. Chi-square was used to compare the complication rates between the 2 groups. Results: A total of 8609 patients underwent PLIF procedure with or without rhBMP2. Individual complication rates in the rhBMP2 group ranged from 0.45% to 7.68% compared with 0.65% to 10.99 in the non-rhBMP2 group. Complication rates for cardiac, pulmonary, lumbosacral neuritis, infection, wound, and urinary tract (include acute kidney failure and post-operative complications) were significantly lower in the rhBMP2 group ( P < .05). There was no difference in the rates of central nervous system complications or radiculitis between the 2 groups. Conclusion: Our data showed that the patients who received rhBMP2 had lower complication rates compared to the non-rhBMP2 group. However, use of rhBMP2 was associated with a higher rate of pseudarthrosis. We did not observe any difference in radiculitis and central nervous system complications between the groups.
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Por, Yong-Chen, Carlos Raul Barceló, Kenneth E. Salyer, David G. Genecov, Karen Troxel, El Gendler, Mohammed E. Elsalanty, and Lynne A. Opperman. "Bone Generation in the Reconstruction of a Critical Size Calvarial Defect in an Experimental Model†." Annals of the Academy of Medicine, Singapore 36, no. 11 (November 15, 2007): 911–19. http://dx.doi.org/10.47102/annals-acadmedsg.v36n11p911.

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Objective: This study was designed to investigate the optimal combination of known osteogenic biomaterials with shape conforming struts to achieve calvarial vault reconstruction, using a canine model. Methods: Eighteen adolescent beagles were divided equally into 6 groups. A critical size defect of 6 x 2 cm traversed the sagittal suture. The biomaterials used for calvarial reconstruction were demineralised perforated bone matrix (DBM), recombinant human bone morphogenetic protein-2 (rhBMP2) and autogenous platelet-rich plasma (PRP). The struts used were cobalt chrome (metal) or resorbable plate. The groupings were as follows: 1) DBM + metal, 2) DBM + PRP + metal, 3) DBM + PRP + resorbable plate, 4) DBM + rhBMP2 + metal, 5) DBM + rhBMP2 + PRP + metal, and 6) DBM + rhBMP2 + resorbable plate. Animals were euthanised at 3 months post-surgery. There was no mortality or major complications. Analysis was performed macroscopically, histologically, and with computed tomography (CT). Results: There was complete bony regeneration in the rhBMP2 groups only. Non-rhBMP2 groups had minimal bony ingrowth from the defect edges and on the dural surface, a finding confirmed by CT scan and histology. PRP did not enhance bone regeneration. Shape conformation was good with both metal and resorbable plate. Conclusion: rhBMP2 but not PRP accelerated calvarial regeneration in 3 months. The DBM in the rhBMP2 groups were substituted by new trabecular bone. Shape molding was good with both metal and resorbable plate. Key words: Critical size calvarial defect, Cranial vault reconstruction, Metal struts, Resorbable plates, rhBMP2
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Patel, Harshadkumar A., Ian J. Wellington, Klair Lubonja, John W. Stelzer, Christopher L. Antonacci, Ergin Coskun, Mark P. Cote, Hardeep Singh, Scott S. Mallozzi, and Isaac L. Moss. "Current Trends in Recombinant Human Bone Morphogenetic Protein 2 (rhBMP2) Usage for Spinal Fusion Surgery." Medicina 59, no. 5 (May 3, 2023): 878. http://dx.doi.org/10.3390/medicina59050878.

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(1) Background: Since first approved by the FDA, on-label and off-label usage of recombinant human bone morphogenetic protein 2 (rhBMP2) for spinal fusion surgeries has become widespread. While many studies have investigated the safety and efficacy of its use, as well as its economic impact, few have looked at the current trends in its on- and off-label use. The goal of this study is to evaluate the current trends of on- and off-label rhBMP2 use for spinal fusion surgery. (2) Methods: A deidentified survey was created and electronically distributed to members of two international spine societies. Surgeons were asked to report their demographic information, surgical experience, and current usage of rhBMP2. They were then presented with five spinal fusion procedures and asked to report if they use rhBMP2 for these indications in their current practice. Responses were stratified between rhBMP2 users vs. non-users and on-label vs. off-label use. Data were analyzed using chi-square with Fisher’s exact test for categorical data. (3) Results: A total of 146 respondents completed the survey with a response rate of 20.5%. There was no difference in overall rhBMP2 usage based on specialty, experience, or number of cases per year. Fellowship-trained surgeons and those who practice in the United States were more likely to use rhBMP2. Surgeons who were trained in the Southeast and Midwest regions reported the highest usage rates. rhBMP2 use was more common among fellowship-trained and US surgeons for ALIFs; non-US surgeons for multilevel anterior cervical discectomy and fusions; and fellowship-trained and orthopedic spine surgeons for lateral lumbar interbody fusions. Non-US surgeons were more likely to use rhBMP2 for off-label indications compared to surgeons from the US. (4) Conclusions: While various demographics of surgeons report different rates of rhBMP2 use, off-label use remains relatively commonplace amongst practicing spine surgeons.
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Fujioka-Kobayashi, Masako, Mustafa Abd El Raouf, Nikola Saulacic, Eizaburo Kobayashi, Yufeng Zhang, Benoit Schaller, and Richard J. Miron. "Superior bone-inducing potential of rhBMP9 compared to rhBMP2." Journal of Biomedical Materials Research Part A 106, no. 6 (February 19, 2018): 1561–74. http://dx.doi.org/10.1002/jbm.a.36359.

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Fujioka‐Kobayashi, Masako, Mustafa Abd El Raouf, Nikola Saulacic, Eizaburo Kobayashi, Yufeng Zhang, Benoit Schaller, and Richard J. Miron. "Superior bone‐inducing potential of rhBMP9 compared to rhBMP2." Journal of Biomedical Materials Research Part A 107, no. 6 (May 3, 2019): 1351. http://dx.doi.org/10.1002/jbm.a.36591.

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Zhao, He, Yali Ma, Dahui Sun, Wendi Ma, Jihang Yao, and Mei Zhang. "Preparation and Characterization of Coaxial Electrospinning rhBMP2-Loaded Nanofiber Membranes." Journal of Nanomaterials 2019 (August 29, 2019): 1–13. http://dx.doi.org/10.1155/2019/8106985.

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DEX and rhBMP2-loaded core-shell nanofiber membranes were synthesized by electrospinning method in one step. Zein/PLLA, Zein-DEX/PLLA, Zein/PLLA-rhBMP2, and Zein-DEX/PLLA-rhBMP2 were fabricated; and morphology, hydrophilicity, mechanics properties, in vitro drug release behavior, cell proliferation, and osteogenic differentiation were investigated. The results showed that the dual-release system containing rhBMP2 and DEX prepared by electrospinning had rough surface, constant drug release behavior, and could also significantly promote cell proliferation and osteogenic differentiation of RMSCs, indicating that the scaffolds we fabricated might be suitable for bone tissue engineering.
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Lao, Lifeng, Jeremiah R. Cohen, Zorica Buser, Darrel S. Brodke, S. Tim Yoon, Jim A. Youssef, Jong-Beom Park, Hans-Joerg Meisel, and Jeffrey C. Wang. "Trends Analysis of rhBMP2 Utilization in Single-Level Anterior Lumbar Interbody Fusion in the United States." Global Spine Journal 8, no. 2 (May 16, 2017): 137–41. http://dx.doi.org/10.1177/2192568217701119.

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Study Design: Retrospective case study. Objective: To evaluate the trends and demographics of recombinant human bone morphogenetic protein 2 (rhBMP2) utilization in single-level anterior lumbar interbody fusion (ALIF) in the United States. Methods: Patients who underwent single-level ALIF from 2005 to 2011 were identified by searching ICD-9 diagnosis and procedure codes in the PearlDiver Patient Records Database (PearlDiver Technologies, Fort Wayne, IN), a national database of orthopedic insurance records. The year of procedure, age, gender, and region of the United States were analyzed for each patient. Results: A total of 921 patients were identified who underwent a single-level ALIF in this study. The average rate of single-level ALIF with rhBMP2 utilization increased (35%-48%) from 2005 to 2009, but sharply decreased to 16.7% in 2010 and 15.0% in 2011. The overall incidence of single-level ALIF without rhBMP2 (0.20 cases per 100 000 patients) was more than twice of the incidence of single-level ALIF with rhBMP2 (0.09 cases per 100 000 patients). The average rate of single-level ALIF with rhBMP2 utilization is highest in West (41.4%), followed by Midwest (33.3%), South (26.5%) and Northeast (22.2%). The highest incidence of single-level ALIF with rhBMP2 was observed in the group aged less than 65 years (compared with any other age groups, P < .001), with an incidence of 0.21 per 100 000 patients. Conclusions: The incidence of rhBMP2 utilization in single-level ALIF increased from 2006 to 2009, but decreased in 2010 and 2011. The Northeast region had the lowest incidence of rhBMP2 utilization. The group aged less than 65 years trended to have the higher incidence of single-level ALIF with rhBMP2 utilization.
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Esmail, Nabil, Zorica Buser, Jeremiah R. Cohen, Darrel S. Brodke, Hans-Joerg Meisel, Jong-Beom Park, Jim A. Youssef, Jeffrey C. Wang, and S. Tim Yoon. "Postoperative Complications Associated With rhBMP2 Use in Posterior/Posterolateral Lumbar Fusion." Global Spine Journal 8, no. 2 (April 20, 2017): 142–48. http://dx.doi.org/10.1177/2192568217698141.

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Study Design: Retrospective database review. Objective: Posterior/posterolateral lumbar fusion (PLF) is an effective treatment for a variety of spinal disorders; however, variations in surgical technique have different complication profiles. The aim of our study was to quantify the frequency of various complications in patients undergoing PLF with and without human recombinant bone morphogenetic protein 2 (rhBMP2). Methods: We queried the orthopedic subset of the Medicare database (PearlDiver) between 2005 and 2011 for patients undergoing PLF procedures with and without rhBMP2. Complication and reoperation rates were analyzed within 1 year of the index procedure. Complications assessed include: acute renal failure, deep vein thrombosis, dural tear, hematoma, heterotopic ossification, incision and drainage, cardiac complications, nervous system complications, osteolysis, pneumonia, pseudarthrosis, pulmonary embolism, radiculopathy, respiratory complications, sepsis, urinary retention, urinary tract infection, mechanical, and wound complications. Chi-square analysis was used to calculate the complication differences between the groups. Results: Our data revealed higher overall complication rates in patients undergoing PLF with rhBMP2 versus no_rhBMP2 (76.9% vs 68.8%, P < .05). Stratified by gender, rhBMP2 males had higher rates of mechanical complications, pseudarthrosis, and reoperations compared with no_rhBMP2 males ( P < .05), whereas rhBMP2 females had higher rates of pseudarthrosis, urinary tract infection, and urinary retention compared with no_rhBMP2 females ( P < .05). Conclusion: Our data revealed higher overall complication rates in PLF patients given rhBMP2 compared with no_rhBMP2. Furthermore, our data suggests that rhBMP2-associated complications may be gender specific.
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Dissertations / Theses on the topic "Rhbmp2"

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Valenzuela, Juan Carlos Bustos. "Análise dos genes diferencialmente expressos durante a osteodiferenciação induzida por proteínas morfogenéticas de osso (BMP2 e BMP7) em células C2C12 e super-expressão de rhBMP2 e rhBMP7 em células de mamíferos." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-31072009-140027/.

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As BMPs (Bone Morphogenetic Proteins) são membros da superfamília de proteínas TGF-β (Transforming Growth Factor β ), regulam o crescimento e diferenciação de vários tipos celulares em diversos tecidos, e algumas delas desempenham um papel crítico na diferenciação de células de origem mesenquimal em osteoblastos. Particularmente, rhBMP2 e rhBMP7, promovem osteoindução tanto \"in vitro\" como \"in vivo,\" sendo, ambas as proteínas utilizadas terapeuticamente em Ortopedia/Odontologia para reparo ósseo. A expressão diferencial de genes durante a osteodiferenciação de células C2C12 induzida por rhBMP2 e rhBMP7, foi analisada através de microarranjos de DNA, selecionando 31 genes, dos quais 24 foram validados por qPCR, 13 dos quais são relacionados à transcrição, quatro associados a algumas vias de sinalização celular e sete associados à matriz extracelular. Análise funcional destes genes permitirá conhecer, com maiores detalhes, os eventos moleculares que ocorrem durante a diferenciação osteoblástica de células C2C12 induzida por rhBMPs. Em paralelo, foi perseguida a super-expressão de rhBMP2 e rhBMP7 em células HEK293T, demonstrando-se a atividade de rhBMP7, induzindo osteodiferenciação \"in vitro\" e formação de osso \"in vivo\", demonstrando a viabilidade do objetivo de se produzir estas proteínas para futura aplicação como biofármacos no Brasil.
The BMPs (Bone Morphogenetic Proteins) are members of the TGF-β (Transforming Growth Factor β) superfamily of proteins, regulate growth and differentiation of various cell types in various tissues, and some play a critical role in differentiation of mesenchymal cells into osteoblasts. Particularly, rhBMP2 and rhBMP7, promote osteoinduction \"in vitro\" and \"in vivo\" and both proteins are used therapeutically in Orthopedics and Dentistry. The differential expression of genes during osteodifferentiation induced by rhBMP2 and rhBMP7 in C2C12 cells was analyzed through DNA microarrays, allowing the selection of 31 genes, of which 24 were validated by qPCR, 13 of which are related to transcription, four associated with cell signaling pathways and seven are associated with the extracellular matrix. Subsequent functional analysis of these genes should reveal more details on the molecular events which take place during C2C12 cells osteoblastic differentiation induced by rhBMPs In paralel, rhBMPs 2 and 7 were overexpressed in HEK293T cells and BMP7 activity to induce osteodifferentiation \"in vitro\" and bone formation \"in vivo\" was demonstrated, reinforcing the viability of our objective to produce these proteins for future application as biopharmaceuticals in Brazil.
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LECOEUR, ALAIN DIT LOIC. "Etude de l'induction de la differenciation osteoblastique sur les cellules stroma-vasculaires de tissu adipeux extramedullaire en culture sous l'influence de la proteine recombinante humaine rhbmp2 et de la dexamethasone : chez le lapin et chez l'humain." Paris 7, 1997. http://www.theses.fr/1997PA07GA06.

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Gonzaga, Miliane Gonçalves. "Estudo qualitativo e quantitativo da interação entre a proteína morfogenética rhBMP-2 e diferentes tipos de enxertos ósseos." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/17/17142/tde-29052015-110800/.

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As proteínas com potencial osteoindutor, com destaque a rhBMP-2, possuem amplos efeitos sobre o crescimento e a diferenciação celular. Este estudo, teve o objetivo avaliar a relação entre diferentes enxertos ósseos e a rhBMP-2 no reparo de defeitos ósseos críticos em calvárias de ratos. Foram utilizados 56 ratos Wistar albinos, machos, aproximadamente 250g, com um período de espera de 6 semanas desde a criação do defeito até o sacrifício, divididos em 8 grupos (n=7): enxerto autólogo (AUT); enxerto homólogo (HOM); enxerto heterólogo (HET); enxerto autólogo e 5 g rhBMP-2 (AUT BMP); enxerto homólogo e 5 g rhBMP-2 (HOM BMP); enxerto heterólogo e 5 g rhBMP-2 (HET BMP); 5 g rhBMP-2 (BMP) e defeito ósseo crítico apenas (DOC). Os resultados foram avaliados estatisticamente pelo teste ANOVA e pós-teste de TUKEY com múltiplas comparações (nível de significância de p<0,05), após o emprego de metodologias de análise histológica qualitativa, histomorfometria e micro CT. Na quantificação do volume ósseo neoformado, todos os grupos obtiveram melhores resultados que DOC com diferença significante. Os grupos HOM e HET apresentaram diferença significante de AUT BMP, HOM BMP e HET BMP que tiveram maior formação óssea enquanto que AUT apresentou diferença significante de HOM BMP e HET BMP. O grupo BMP não teve diferença significante em relação a AUT, HOM, HET, AUT BMP, HOM BMP e HET BMP. Os grupos AUT, HOM e HET não tiveram diferença significante entre si da mesma forma que os grupos AUT BMP, HOM BMP e HET BMP também não o tiveram. Quanto à quantificação de osteoclastos, não houve diferença significante entre os grupos; porém, HET BMP teve mais células osteoblásticas que DOC com diferença significante. Considerando os osteócitos, DOC e AUT apresentaram poucas células. Assim, DOC teve diferença significante de BMP, HET, HOM BMP e HET BMP e AUT teve diferença significante de BMP, HET, HOM BMP e HET BMP. Da mesma forma, AUT BMP teve diferença significante em relação a HET que apresentou mais células. Na quantificação de fibras colágenas, não houve diferença significante entre os grupos. A neoformação óssea progrediu das bordas para o centro do defeito e no grupo BMP o osso neoformado ocupou mais de 2/3 do defeito. No grupo DOC, foi constatada uma faixa estreita de tecido ósseo neoformado nas bordas e o restante do defeito foi preenchido por tecido conjuntivo. Nas imagens de micro CT, todos os grupos apresentaram maior formação óssea que o grupo DOC; sendo que AUT superou HOM e HET. Além disso, os diferentes enxertos associados à rhBMP-2 apresentaram maior formação óssea que os respectivos enxertos usados sem a proteína, com destaque para AUT BMP. Diante disso, foi possível concluir que a rhBMP-2 auxiliou o reparo ósseo quando administrada isoladamente ou associada aos diferentes enxertos ósseos, conferindo neste caso melhores resultados
Proteins with osteoinductive potential, particularly the rhBMP-2, have great effects on growth and cell differentiation. This study aimed to evaluate the relationship between different bone grafts and rhBMP-2 on the repair of critical bone defects in rat skulls. Fifty six Wistar rats, male, approximately 250g of body weight were used, sacrificed six weeks after surgical experiment, and divided into 8 groups(n=7): autograft (AUT); homograft (HOM); xenograft (HET); autograft and 5 g rhBMP-2 (AUT BMP); homograft and 5 g rhBMP-2 (HOM BMP); xenograft and 5 g rhBMP-2 (HET BMP); 5 g rhBMP-2 (BMP) and critical bone defect only (DOC). The results were analyzed statistically by ANOVA and Tukey post-test with multiple comparisons (p<0.05 of significance level), after the use of qualitative methodologies, micro CT morphology and histological analysis. According to the quantification of newly formed bone volume, all groups had better results than DOC group with a significant difference. The HOM and HET groups showed significant difference than AUT BMP, HOM BMP and HET BMP, that had higher bone formation, while AUT group was significantly different from HOM BMP and HET BMP. The BMP group had no significant difference in relation to AUT, HOM, HET, AUT BMP, HOM BMP and HET BMP. The AUT, HOM and HET groups had no significant difference between them, and also the groups AUT BMP, HOM BMP and HET BMP between them. Regarding the quantification of osteoclasts, there was no significant difference between groups; however, HET BMP had more osteoblastic cells than DOC group, with significant difference. For osteocytes quantification, AUT and DOC showed few cells. Thus, DOC had significant difference in relation to BMP, HET, HOM BMP and HET BMP; and AUT had significant difference in relation to BMP, HET, HOM BMP and HET BMP. Likewise, AUT BMP had significant difference compared to HET group, which showed more cells. Regarding to collagen fibers quantification, there was no significant difference between groups. New bone formation progressed from the edges to the center of the defect and for BMP group the newly formed bone occupied more than two thirds of the defect. DOC group showed a narrow band of newly formed bone at the edges and the remainder of the defect was filled by connective tissue. For micro CT analysis, all groups showed greater bone formation than the DOC group, being greater in AUT group than HOM and HET. Furthermore, the various grafts associated with rhBMP-2 showed greater bone formation than the isolated grafts, highlighting the AUT BMP. Therefore, it was concluded that rhBMP-2 helped bone repair when administered alone or associated with different bone grafts, giving better results when combined to the grafts
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Freitas, Maria da Conceição Andrade de. "Análise histológica da região alveolar enxertada com rhBMP-2 em pacientes com fissuras labiopalatinas." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/61/61132/tde-06062017-085349/.

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Objetivo: Este estudo visou avaliar histologicamente os tecidos ósseo e mole removidos da região da fissura alveolar enxertada com rhBMP-2 em pacientes com fissuras labiopalatinas, 6 a 46 meses após a cirurgia. Material e Metodos: O grupo experimental foi composto por 13 pacientes com caninos retidos na região alveolar enxertada com rhBMP-2 (Infuse®). O grupo controle foi composto por seis pacientes com caninos retidos na região alveolar contralateral ou próxima à fissura não enxertada e um com canino retido na área enxertada com osso autógeno da crista ilíaca. No ato da exposição cirúrgica do canino, 6 a 46 meses após o enxerto ósseo secundário, as biopsias dos tecidos ósseo e mole foram retiradas e submetidas a análise histológica pela técnica hematoxilina e eosina. Resultados: Os cortes microscópicos do grupo experimental (n=12) evidenciaram fragmentos de tecido ósseo viável, com osteócitos normais em lacunas, osteoblastos na superficie óssea e espaços medulares preenchidos por tecido conjuntivo fibroso e vasos sanguineos. O quadro histológico das biópsias de tecido ósseo removidas num período de 6 a 9 meses de enxertia evidenciaram trabéculas ósseas mais desorganizadas, sem formações lamelares e com maior densidade de osteócitos por área indicando menor grau de maturidade óssea. As do período 24 a 46 meses eram compostas somente por tecido ósseo maduro de arranjo lamelar contendo linhas de reversão/incrementais e lacunas de osteócitos. Os cortes de tecido mole (n=3) reveleram fragmentos de mucosa bucal constituída por epitelio pavimentoso estratificado paraqueratinizado com tecido conjuntivo subjacente denso. Conclusão. Os fragmentos de tecidos ósseo e mole removidos da área da fissura enxertada com rhBMP-2 apresentaram morfologia histológica normal, assemelhando-se com as características teciduais do grupo controle.
Objective: This study aimed to evaluate histologically the bone and soft tissue removed from the region of the rhBMP-2-grafted alveolar cleft in patients with cleft lip and palate 6 to 46 months after surgery. Material and Methods: The experimental group consisted of 13 patients with canines retained in the alveolar region grafted with rhBMP-2 (Infuse®). The control group consisted of six patients with canines retained in the contralateral alveolar region or near the ungrafted cleft and one with canine retained in the grafted area with autogenous iliac crest bone. At the time of surgical exposure of the canine, 6 to 46 months after secondary bone graft, biopsies of bone and soft tissues were submitted to histological analysis by the hematoxylin and eosin technique. Results: The microscopic sections (n = 12) showed fragments of viable bone tissue with normal osteocytes in gaps, osteoblasts in the bone surface and spinal spaces filled by fibrous connective tissue and blood vessels. The bone tissue biopsies removed at a 6 to 9 months after grafting showed more disorganized bone trabeculae, with no lamellar formations and with a higher osteocyte density per area indicating a lower degree of bone maturity. Biopsies taken from 24 to 46 months after grafting were composed only for mature lamellar bone tissue containing reversion / incremental lines and osteocyte gaps. Soft tissue sections (n= 3) revealed fragments of buccal mucosa consisting of parakeratinized stratified squamous epithelium with fibrous connective tissue. Conclusion. Fragments of bone and soft tissues removed from the rhBMP-2 grafted cleft area showed normal histological morphology, resembling tissue characteristics on the control group.
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Leal, Claudia Resende. "Enxerto alveolar com proteína morfogenética óssea (rhBMP-2) na fissura labiopalatina: influência da idade, do cirurgião, do tipo e da amplitude da fissura." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/61/61132/tde-17102016-142925/.

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Objetivo: Avaliar se a fase de irrupção dos caninos na época da cirurgia, o tipo e a amplitude da fissura labiopalatina e o cirurgião influenciam o resultado das cirurgias de enxerto alveolar realizado com proteína morfogenética óssea (rhBMP-2). Material e métodos: Este estudo transversal avaliou uma amostra de 90 indivíduos submetidos consecutivamente à cirurgia de enxerto alveolar com rhBMP-2 em um único Centro de Reabilitação. As radiografias periapicais foram tomadas antes e 6 meses após a cirurgia. Os enxertos foram realizados por 4 cirurgiões experientes. O resultado dos enxertos alveolares foi caracterizado em sucesso ou insucesso por 3 avaliadores que se basearam nos critérios adotados pelas escalas de Bergland modificada e de Chelsea. A fase de irrupção dos caninos na época do procedimento, o tipo e a amplitude da fissura labiopalatina e o cirurgião que o realizou foram considerados. A maior amplitude da fissura foi mensurada em radiografias periapicais realizadas no pré-operatório usando o aparelho VISTA scan perio-plus (Dürr Dental, Bietigheim-Bissingen, Alemanha). Para a análise de concordância entre os avaliadores foi aplicado o índice Kappa intra e inter-avaliadores. A concordância intra-examinador para a variável amplitude da fissura foi verificada pelo cálculo do erro casual (Dahlberg), do erro sistemático (teste t pareado) e pelo Coeficiente de Correlação Intraclasse (CCI). A influência dos fatores avaliados no resultado do enxerto alveolar foi analisada por meio da Regressão Logística Multivariada (p<0,05). Resultados: Todas as variáveis independentes analisadas apresentaram significância estatística. O grupo caninos não irrompidos mostrou melhores resultados do que o grupo caninos irrompidos (p=0,001). O grupo fissura incompleta demonstrou melhores resultados que o grupo fissura completa (p=0,000). Quanto maior a amplitude da fissura labiopalatina, menos favoráveis foram os resultados do enxerto (p=0,027). O fator cirurgião também influenciou significativamente o sucesso da cirurgia (p=0,003 e 0,001). Conclusão: A fase de irrupção do canino, o tipo e a amplitude da fissura labiopalatina e o cirurgião influenciaram o resultado das cirurgias de enxerto alveolar realizado com rhBMP-2.
Objective: To evaluate whether the canine irruption stage at the time of surgery, the type and width of cleft lip and palate and the surgeon influence on the outcome of alveolar graft surgeries performed with bone morphogenetic protein (rhBMP-2). Material and methods: This cross-sectional study evaluated a sample 90 individuals who consecutively underwent alveolar graft surgery with rhBMP-2 in a single center. Periapical radiographs were taken before and 6 months after surgery. Four experienced surgeons operated all patients. The result of alveolar graft was characterized in success or failure by three raters based on the criteria adopted by both the modified Bergland and Chelsea scales. The canine irruption stage at the time of the procedure, the type and width of cleft lip and palate and the surgeon who performed the surgery were considered. The larger width of the alveolar cleft was measured on periapical radiographs taken preoperatively using the software VISTA perio-plus scan (Dürr Dental, Bietigheim-Bissingen, Germany). Intra and inter-rater agreements were evaluated using Kappa coefficient. The intra-rater agreement for the variable cleft width was evaluated using Dahlberg test, paired t-tests and the Intraclass Correlation Coefficient (ICC). The influence of the independent variables in the outcome of alveolar graft was analyzed using Multivariate Logistic Regression (p<0.05). Results: All independent variables were statistically significant. The unerupted canine group showed better results than the erupted canines group (p=0.001). Cleft lip and alveolus group showed better outcomes than the complete cleft lip and palate group (p = 0.000). The larger the alveolar cleft width, the poorer the outcome of the alveolar graft (p = 0.027). The surgeon also had a significant influence on the success of the surgery (p = 0.003 and 0.001). Conclusion: The canine irruption stage, the type and width of cleft lip and palate and the surgeon influenced the outcome of alveolar graft surgeries performed with rhBMP-2.
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Kotake, Bruna Gabriela dos Santos. "Avaliação da reparação óssea em defeitos críticos após a aplicação da proteína rhBMP-2 pura e/ou combinada em animais normais e sob efeito do alcoolismo crônico." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/58/58137/tde-04122012-164616/.

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O alcoolismo é considerado um redutor da formação óssea, podendo levar a distúrbios osteometabólicos, já a rhBMP-2 é uma proteína morfogenética conhecida por desempenhar um papel importante nos processos de reparação e indução da formação óssea. O objetivo deste estudo foi investigar a ação do alcoolismo e a resposta do reparo em defeitos ósseos (DO) na calvária de ratos, após a aplicação da rhBMP-2, pura e combinada a uma matriz de colágeno. Foram utilizados 80 ratos divididos em 8 grupos, cada um deles com um período de espera até o sacrifício de 4 e 6 semanas, após a criação cirúrgica do defeito ósseo na calvária de 5 mm. Os grupos foram divididos em G1) água \"ad libitum\" + DO, G2) álcool \"ad libitum\" + DO, G3) água + DO + 5μg rhBMP-2 pura, G4) álcool + DO + 5μg rhBMP-2 pura, G5) água + DO + carreador esponja de colágeno, G6) álcool + DO + carreador esponja de colágeno, G7) água e + DO + 5μg rhBMP- 2/esponja de colágeno, G8) álcool + DO + 5μg rhBMP-2/esponja de colágeno. Os dados radiográficos e os dados para a análise de fibras colágena tipo I e III foram submetidos à análise estatística Kruskal-Wallis e Dunn\'s Multiple, os dados histológicos ao teste de análise de variância (ANOVA) e Tukey. O resultado encontrado para a análise radiográfica no tempo de 6 semanas demonstrou que os grupos tratados com rhBMP-2 independente da utilização do carreador e do etanol apresentaram maior neoformação óssea (p<0,05), para o tempo de 4 semanas não foi encontrada diferença estatística. Para a análise imunoistoquímica, a ostecalcina (OC) e sialoproteína óssea (BPS) foram predominantes nos grupos tratados com rhBMP-2. Para a análise histológica, a quantificação de fibras colágenas tipo I apresentou diferença estatística entre os grupos (p<0,05), com aumento destas fibras principalmente nos grupos tradados com rhBMP-2 associado a esponja de colágeno; e na análise quantitativa por estereologia, o volume de tecido ósseo neoformado foi maior para os grupos tratados com rhBMP-2 pura ou associada ao carreador, porém para os grupos tratados com etanol, houve maior neoformação óssea para o grupo tratado com rhBMP-2 associado ao carreador nos períodos de 4 e 6 semanas (p<0,001). Concluiu-se neste modelo experimental que, o carreador foi efetivo na bioliberação da rhBMP-2, mesmo na presença do etanol.
Alcoholism is considered a reducer for new bone formation, may leading to osteometabolic disturbance, considering that the rhBMP-2 is a morphogenetic protein known to play an important role in the bone healing processes. The aim of this study was to investigate the action of alcoholism and its effect on the repair of bone defects (DO) performed in rat calvaria after the rhBMP-2 application, pure or combined with a collagen matrix. We used 80 rats divided into eight groups, each one with a waiting period for sacrifice of 4 and 6 weeks after the bone defect (5mm) delineation in the rat skull. The groups were divided into: G1) water \"ad libitum\" + DO, G2) alcohol \"ad libitum\" + DO, G3) water + DO + 5μg pure rhBMP-2, G4) alcohol + DO + 5μg pure rhBMP-2, G5 ) DO + water + collagen sponge carrier, G6) alcohol + DO + collagen sponge carrier, G7) and water + DO + 5μg rhBMP-2/collagen sponge, G8) alcohol + DO + 5μg of rhBMP-2/collagen sponge. The radiographic data were submitted to statistical analysis Kruskal-Wallis and Dunn\'s Multiple, histological data to analysis of variance (ANOVA) and Tukey test. Radiographically, it was found after 6 weeks that the groups treated with rhBMP-2, independently of the carrier use and ethanol administration, more new bone formation (p<0.05), at 4 weeks it was not found statistical difference. For immunohistochemical analysis, ostecalcin (OC), and bone sialoprotein (BPS) were predominant in groups treated with rhBMP-2. For histological quantification of collagen type I fibers, statistical difference between groups was found (p<0.05) with the increasing of these fibers in the groups treated with rhBMP-2 combined with collagen sponge; and quantitative by stereology, aiming to calculate the volume of new bone, it was found higher values for the groups treated with rhBMP-2 pure or combined to the carrier; but for the groups treated with ethanol, it was found higher bone formation in the groups treated with rhBMP-2 associated to the carrier in the periods of 4 and 6 weeks (p <0.001). It was concluded in this experimental model that the carrier was effective for rhBMP-2 delivery, even in the presence of ethanol.
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Izadpanah, Ali. "Effect of rhBMP-7 dosing during distraction osteogenesis in wild type mice." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=103541.

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Distraction osteogenesis (DO) is a common surgical technique for bone lengthening in treatment of limb discrepancies, congenital deformities, traumatic bone loss, infections, and malignancies. It is made of three main phases: the latency phase, the distraction phase, and the consolidation phase. DO is not short of drawbacks. The main drawback of DO is the long consolidation period when the patient is required to keep on the fixator in place for the regenerate bone to consolidate. Thus, any attempt to shorten this process would be greatly beneficial. Bone morphogenetic proteins (BMPs) are crucial in bone and cartilage development. BMP-2, -4, -6, and -7 have been described as the most osteogenic BMPs. BMP-7 has been recently demonstrated to be beneficial in bone formation during distraction osteogenesis in a rat model. However, there are no studies investigating the optimal dose of BMP-7 administration for accelerating bone formation during distraction osteogenesis. Hence, we sought to determine the optimal dose of exogenous BMP-7 administration during DO. For this purpose, DO was performed on the right tibia of 79 wild type male mice (C57BL/6) using a miniature version of the Ilizarov fixator. Mice underwent a latency period of 5 days, followed by a distraction phase of 12 days (0.2 mm every twelve hours) and a consolidation phase of 34 days. Animals were randomized into two major groups according to the time of sacrifice: mid-consolidation (day 34) and end of consolidation (day 51). Each major group was further sub-divided according to the administered dose of BMP-7. Three different doses of BMP-7 were injected at the osteotomy site on the first day of distraction (0.05µg, 0.10µg, 0.50µg corresponding to 2µg/kg, 4µg/kg, and 20µg/kg of BMP-7). Control groups (6 mice for mid-consolidation and 4 mice for end of consolidation) were injected with ultra-pure water. Collected samples were studied using Faxitron X-ray, µCT, static histomorphometry, biomechanical testing, immunohistochemistry, and histology. We saw a reducing effect in bone formation during distraction osteogenesis of wild type mice upon increasing the dose of BMP-7 at mid-consolidation. µCT analysis and static histomorphometry parameters revealed a statistically significant decrease in bone volume/tissue volume ratio (BV/TV) and new regenerate BV upon increasing the BMP-7 dose. However, all groups had greater BV/TV and new regenerate bone volume compared to the control group. Biomechanical evaluation at 34 days showed the greatest stiffness and ultimate work to fracture at the lowest administrated dose. On the other hand, at the end of consolidation, the µCT and static histomorphometry results demonstrated the greatest BV/TV at medium dose (30.09±4.076, 38.73±1.533, 27.02±2.684, and 17.68±3.808 for 2µkg, 4µg/kg, 20µg/kg, and control respectively). Similarly, the new regenerate bone formation had comparable results (4.062±0.4874, 6.969±1.123, and 4.946±0.6362 for 2µkg, 4µg/kg, and 20µg/kg respectively). Biomechanical testing of collected samples at 51 days (end of consolidation) demonstrated a gradual decrease in stiffness (63.72±35.03, 45.56±8.045, 28.99±11.22, and 35.49±17.00 for 2µkg, 4µg/kg, 20µg/kg, and control respectively) and ultimate force to fracture (13.69±5.149, 13.12±2.086, 8.815±2.972, and 8.188±3.334 for 2µkg, 4µg/kg, 20µg/kg, and control respectively) upon increasing the administered dose of BMP-7. In conclusion, results from this study suggest that BMP-7 exerts an important physiological role in new bone regeneration during distraction osteogenesis in wild type mice. Additionally, we demonstrated that upon increasing the administered BMP-7 dosing, the amount of new regenerate bone formation and BV/TV has a decreasing trend. These results were comparable with the biomechanical evaluations obtained at mid-consolidation and end of consolidation. Thus, the dose-response curve does plateau and proceeds downward at higher dosages of exogenous rhBMP-7.
L'ostéogenèse par distraction (DO) est une technique courante de chirurgie pour l'allongement des os dans le traitement des anomalies des membres, des malformations congénitales, de la perte osseuse traumatique, des infections et des tumeurs malignes. Elle est composée de trois phases principales: la phase de latence, la phase de distraction, et la phase de consolidation. DO amène cependant plusieurs inconvénients. Le principal inconvénient de l'ostéogenèse par distraction est la longue période de solidification. Ainsi, toute tentative visant à raccourcir ce processus serait grandement bénéfique. Les protéines morphogénétiques osseuses (BMP) sont cruciales au développement des os et du cartilage. Il a récemment été démontré que la BMP-7 a un effet bénéfique sur la formation osseuse au cours de distraction osseuse sur des rats. Cependant, il n'existe pas d'études pour trouver la dose optimale d'injection de BMP-7. Par conséquent, nous avons cherché à déterminer la dose optimale d'administration de BMP-7 exogène au cours de DO. À cette fin, DO a été effectuée sur le tibia droit de 79 souris mâle sauvages (C57BL / 6) en utilisant une version miniature du fixateur Ilizarov. Les animaux ont été séparés au hasard en deux groupes principaux en fonction du moment d'euthanasie; 45 souris ont été euthanasiées à la mi-solidification et 34 ont été euthanasiées à la fin de la solidification. Chaque groupe a ensuite été subdivisé en fonction de la dose administrée de BMP-7. Trois doses différentes de BMP-7 ont été injectées sur le site de l'ostéotomie au premier jour de la distraction (0.05μg, 0.10μg, 0.50μg correspondant à 2μg/kg, 4μg/kg et 20μg/kg de BMP-7). Les groupes de contrôle ont été injectés avec de l'eau pure. Des échantillons ont été collectés à deux points dans le temps: 34 jours (mi-solidification) et 51 jours (fin de la solidification). Les échantillons prélevés ont été étudiés à l'aide d'un rayon-X Faxitron, d'un μCT, de l'histomorphométrie statique, d'essais biomécaniques, de l'immunohistochimie, et de l'histologie. Les résultats ont démontré qu'il y a un effet réducteur pour accélérer la formation osseuse au cours de distraction osseuse avant l'augmentation de la dose de BMP-7 au mi-solidification. L'analyse μCT et les paramètres histomorphométrie statiques ont révélé une diminution statistiquement significative du volume osseux/volume des tissus (BV/TV) et de la régénération osseuse avant l'augmentation de la dose de BMP-7. L'évaluation biomécanique au 34ième jours a également démontré une plus grande rigidité et de travail ultime avant rupture à la plus faible dose administrée de BMP-7. D'autre part, au fin de la solidification, les résultats histomorphométriques statiques et μCT ont démontré la plus grande BV/TV à la dose moyenne d'administration de 4μg/kg (30.09 ± 4,076, 1,533 ± 38,73, 27,02 ± 2,684 et 17,68 ± 3,808 pour 2μkg, 4μg/kg, 20μg/kg, et le contrôle, respectivement). De même, la formation de osseuse avait des résultats comparables (4,062 ± 0,4874, 6,969 ± 1,123, 4,946 et 0,6362 ± pour 2μkg, 4μg/kg et 20μg/kg respectivement). Les essais biomécaniques sur les échantillons prélevés au fin de la solidification ont démontré une diminution progressive de la rigidité (63,72 ± 35,03, 45,56 ± 8,045, 28,99 ± 11,22 et 35,49 ± 17,00 pour 2μkg, 4μg/kg, 20μg/kg, et le contrôle, respectivement) et la force ultime avant rupture (13,69 ± 5,149, 2,086 ± 13,12, 8,815 ± 2,972, 8,188 et 3,334 ± pour 2μkg, 4μg/kg, 20μg/kg, et le contrôle, respectivement) après l'augmentation de la dose. En conclusion, les résultats de cette étude suggèrent que la BMP-7 exerce un rôle physiologique important dans la régénération osseuse au cours de la DO. Également, nous avons démontré qu'avec l'augmentation de la dose administrée, la quantité de formation osseuse et de BV sont à la baisse. Ces résultats sont comparables avec les évaluations biomécaniques obtenus à mi-solidification et fin de la solidification.
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Schwartz, Daniel. "Development of an Aqueous Suspension of Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2)." Diss., lmu, 2005. http://nbn-resolving.de/urn:nbn:de:bvb:19-44316.

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9

Johnson, Trenton. "Effect of guided bone regeneration with rhBMP-2 on bone quality surrounding dental implants." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu152147382891412.

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Lucia, Conrado Ingraci de. "Análise quantitativa dos níveis de cálcio, Colagenase A e B durante o reparo ósseo em calvárias de ratos sob o modelo experimental de defeito ósseo." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/58/58137/tde-03042013-163708/.

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O osso é um tipo especializado de tecido com alto teor mineral e desempenha variadas funções no organismo, como a reserva de cálcio, proteção de estruturas vitais e alavanca para a movimentação dos musculos. Constantemente o osso passa por processos de remodelação, o que mantém sua estrutura funcional e repara pequenas fraturas que ocorrem normalmente devido ao estresse do uso contínuo. O sistema de reparo funciona em perfeita sincronia mediante células que produzem os componentes ésseos e células que os reabsorvem permitindo a organização do tecido. Esse sistema de manuteção depende da interação entre estas células bem como dos sinais enviados por mediadores e moduladores. Varias proteínas funcionam como indutores de formação óssea, mas também no sentido de facilitar essa reconstrução. Dentre estas proteínas se encontram as BMPs, que possuem grande potencial osteoindutor, e MMPs, que atuam em diversas fases da construção e manutenção deste tecido. Particularmente a BMP-2 tem mostrado um potencial significativo em termos de indução e sua forma recombinante a rhBMP-2, produzida por engenharia recombinante, foi liberada para comercialização e utilizacao clínica. Quanto às MMPs, há importante função das MMP-2 e MMP-9 neste tecido. A primeira estruturando a matriz e modulando o processo de reabsorção nos processos inflamatórios inerentes ao reparo; a segunda atuando desde fases iniciais às tardias, produzida principalmente por osteoclastos e utilizada na remodelação do osso novo. Porém, esta capacidade de reparo do osso é limitada e defeitos ósseos de grande extensão exigem muito do organismo, podendo levar a um reparo que não se estrutura devidamente. Assim, várias técnicas foram propostas para estimular o desenvolvimento ósseo e a utilizacao de enxertos se mostrou eficaz para fornecer um arcabouço de crescimento, facilitando a implantacao do osso neofomado e protegendo o leito do defeito durante todo o extenso período de recuperação. O presente estudo enfocou três diferentes tipos de enxerto ósseo (autólogo, homólogo e heterólogo) e suas associações com a proteína rhBMP-2, verificando sob o aspecto bioquímico a relação de cada um com a quantidade de MMP-2 e MMP-9 em dois tempos de reparo diferentes. De maneira geral, verificou-se que no primeiro momento há maior produção de MMP-2 e os níveis de MMP-9 se mantém de forma relativamente constante nos dois tempos cirúrgicos. O enxerto autólogo apresenta melhores resultados, seguido dos obtidos no enxerto homólogo e heterólogo respectivamente, entretanto a adição de rhBMP-2 a estes enxertos não parece influenciar nos níveis de MMP-2 e MMP-9 nos dois períodos. A dosagem de cálcio revelou que se apresentavam mais mineralizados os grupos de enxerto autólogo e homólogo, os demais grupos além de apresentar menores niveis de cálcio, ainda decresceram nestes níveis no segundo período do experimento.
Bone is a special tissue with a high mineral content and performs various functions in the body, such as calcium reserves, protection of vital structures and muscles lever during the movement. Bone constantly undergoes remodeling processes, which keeps its functional structure and repair small fractures that commonly occur due to the stress of continuous use. The repair system works perfectly synchronized by the cells that produce bone components and resorbing cells, allowing the perfect tissue organization. This maintenance system depends on the interaction between these cells and the signals sent by mediators and modulators. Several proteins operate to induce bone formation, but also to facilitate the reconstruction. Among these proteins are the BMPs, which have great osteoinductive potential, and MMPs that act at different stages of construction and maintenance of this tissue. Particularly BMP-2 has shown significant potential in terms of induction and its recombinant form, rhBMP-2, produced by recombinant engineering, has been released for clinical use and commercialization. In relation to MMPs, there are important functions of MMP-2 and MMP-9 in this tissue. First, structuring the matrix and modulating the resorption during inflammatory processes inherent to repair; second, acting at early to later stages, produced mainly by osteoclasts and used during bone remodeling. However, this repair capacity is limited and large bone defects require a lot of strength of the body, may leading to a bone repair not well structured. Thus, several proposed techniques to stimulate the development and use of bone grafts were effective to provide a framework for growth, facilitating the implementation of new bone and protecting the defect bed throughout the extended recovery period. This study focused on three different types of bone graft (autologous, homologous and heterologous) and their association with rhBMP-2 protein, evaluating the biochemical aspects according to the amount of MMP-2 and MMP-9 in two different periods of time. In general, it was found that firstly, there is an increased production of MMP-2, and MMP-9 levels remain relatively constant in both considered periods of time. The autologous graft presented the best results followed by homologous and heterologous, respectively; however the addition of rhBMP-2 in these grafts did not seem to influence the MMP-2 and MMP-9 levels, in both periods of time. The calcium dosage revealed more mineralization at the autologous and homologous groups, the other groups, besides having lower calcium levels, decreased these levels at the second period of this experiment.
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Books on the topic "Rhbmp2"

1

McKay, William F. The rhBMP-2 reference guide. St. Louis, Mo: Quality Medical Pub., 2002.

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Book chapters on the topic "Rhbmp2"

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Voggenreiter, G., K. Hartl, S. Assenmacher, M. Chatzinikolaidou, and H. P. Jennissen. "Biologische Beschichtung von Implantaten mit rhBMP-2." In Deutsche Gesellschaft für Chirurgie, 453–55. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-56698-1_117.

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Herford, Alan, and Isaac Lowe. "The Role of rhBMP-2 in Oral and Maxillofacial Reconstruction." In Regenerative Strategies for Maxillary and Mandibular Reconstruction, 33–41. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-319-93668-0_4.

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Subach, B. R., R. W. Haid, Jr., G. E. Rodts, Jr., and C. A. Petraglia. "Bone Morphogenetic Protein (rhBMP-2): Experimental Review and Clinical Update." In Advances in Spinal Stabilization, 1–13. Basel: KARGER, 2003. http://dx.doi.org/10.1159/000072627.

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Peel, Sean A. F., Aileen J. J. Zhou, Hanje Chen, and Cameron M. L. Clokie. "Development of URIST™ a Multiphasic rhBMP-2 Bone Graft Substitute." In Clinical Applications of Biomaterials, 383–410. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-56059-5_12.

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Alonso, Nivaldo, and Julia Amundson. "Bone Substitute: Alveolar Bone Grafting (ABG) with rhBMP-2 (Recombinant Bone Morphogenic Protein-2)." In Cleft Lip and Palate Treatment, 263–68. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-63290-2_17.

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Um, In Woong. "Demineralized Dentin Matrix (DDM) As a Carrier for Recombinant Human Bone Morphogenetic Proteins (rhBMP-2)." In Advances in Experimental Medicine and Biology, 487–99. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-0947-2_26.

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Yao, Shaohua, Ling Li Zhang, Steven Y. Cheng, and Xing Dong Zhang. "Refolding and Purification of rhBMP-2 Expressed as Inclusion Bodies in E.COLI with Hydroxyapatite Chromatography." In Advanced Biomaterials VI, 661–64. Stafa: Trans Tech Publications Ltd., 2005. http://dx.doi.org/10.4028/0-87849-967-9.661.

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Duan, Bin, William W. Lu, and Min Wang. "Selective Laser Sintered Ca-P/PHBV Nanocomposite Scaffolds with Sustained Release of rhBMP-2 for Bone Tissue Engineering." In Advances in Bioceramics and Porous Ceramics IV, 37–48. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9781118095263.ch5.

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Ruhé, P. Q., O. C. Boerman, F. G. M. Russel, P. H. M. Spauwen, Antonious G. Mikos, and John A. Jansen. "Controlled Release of rhBMP-2 Loaded Poly(DL-lactic-co-glycolic Acid)/ Calcium Phosphate Cement Composites In Vivo." In Bioceramics 18, 973–76. Stafa: Trans Tech Publications Ltd., 2006. http://dx.doi.org/10.4028/0-87849-992-x.973.

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McKay, William F., Steven M. Peckham, and Jeffrey M. Badura. "Development of a novel compression-resistant carrier for recombinant human bone morphogenetic protein-2 (rhBMP-2) and preliminary clinical results." In Bone Morphogenetic Proteins: From Local to Systemic Therapeutics, 7–23. Basel: Birkhäuser Basel, 2008. http://dx.doi.org/10.1007/978-3-7643-8552-1_2.

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Conference papers on the topic "Rhbmp2"

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Piperdi, Sajida, David Geller, Amy Park, So Hak Chung, and Richard Gorlick. "Abstract 1630: Effects of rhBMP-2 on osteosarcoma tumorigenesis." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-1630.

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McKay, B. "Local sustained delivery of recombinant human bone morphogenetic protein-2 (rhBMP-2)." In 2009 Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2009. http://dx.doi.org/10.1109/iembs.2009.5332518.

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Inoue, M., J. Tan, H. Tsujigiwa, Y. Kitamura, K. Hibi, Y. J. Lee, S. Kan, et al. "LONG-TERM OBSERVATION OF HETEROTOPIC BONE FORMATION INDUCED BY rhBMP-2 AND IBM COMPOSITES." In Proceedings of the 12th International Symposium on Ceramics in Medicine. WORLD SCIENTIFIC, 1999. http://dx.doi.org/10.1142/9789814291064_0063.

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Kuboki, Y., H. Li, L. J. Bi, H. Kato, and H. Itoh. "TOOTH EXTRACTION SOCKETS EFFECTIVELY RECOVERED AFTER APPLICATION OF rhBMP-2 COMBINED WITH POROUS PARTICLES OF HYDROXYAPATITE." In Proceedings of the 12th International Symposium on Ceramics in Medicine. WORLD SCIENTIFIC, 1999. http://dx.doi.org/10.1142/9789814291064_0056.

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Ohura, K., N. Matsuda, C. Hamanishi, and S. Tanaka. "BONE DEFECT REPAIR USING SUBCUTANEOUSLY INDUCED BONE BY A BIORESORBABLE CALCIUM PHOSPHATE CEMENT COMBINED WITH rhBMP-2." In Proceedings of the 12th International Symposium on Ceramics in Medicine. WORLD SCIENTIFIC, 1999. http://dx.doi.org/10.1142/9789814291064_0057.

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Geller, David S., So Hak Chung, Wendong Zhang, Sajida Piperdi, Carrie Freeman, Bang Hoang, Rui Yang, Michael Roth, Jonathan Gill, and Richard Gorlick. "Abstract 645: The effect of rhBMP-2 on in vitro osteosarcoma tumorigenesis and on pulmonary growth and development." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-645.

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Ishimoto, Takatsugu, Keisuke Miyake, Masakazu Yashiro, Tsugio Eto, Daisuke Izumi, Kota Arima, Yoshifumi Baba, et al. "Abstract 4340: RHBDF2 in stromal fibroblasts mediates TGF-β signaling and enhances gastric cancer cell invasion via intercellular crosstalk." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-4340.

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Bonello, Philip, and Pham Minh Hai. "Computational Studies of the Unbalance Response of a Whole Aero-Engine Model With Squeeze-Film Bearings." In ASME Turbo Expo 2009: Power for Land, Sea, and Air. ASMEDC, 2009. http://dx.doi.org/10.1115/gt2009-59687.

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The computation of the unbalance vibration response of aero-engine assemblies fitted with nonlinear bearings requires the retention of a very large number of modes for reliable results. This renders most previously proposed nonlinear solvers unsuitable for this application. This paper presents three methods for the efficient solution of the problem. The first method is the recently developed impulsive receptance method (IRM). The second method is a reformulation of the Newmark-Beta method. In addition to these two time-domain methods, a whole-engine receptance harmonic balance method (RHBM) is introduced that allows, for the first time, the frequency domain calculation of the periodic vibration response of a real engine. All three methods use modal data calculated from a one-off analysis of the linear part of the engine at zero speed. Simulations on a realistically-sized representative twin-spool engine model with squeeze-film damper bearings provide evidence that the popular Newmark-Beta method can be unreliable for large order nonlinear systems. The excellent correlation between the IRM and RHBM results demonstrates the efficacy of these two complementary tools in the computational analysis of realistic whole-engine models.
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Hai, Pham Minh, and Philip Bonello. "A Computational Parametric Analysis of the Vibration of a Three-Spool Aero-Engine Under Multi-Frequency Unbalance Excitation." In ASME Turbo Expo 2010: Power for Land, Sea, and Air. ASMEDC, 2010. http://dx.doi.org/10.1115/gt2010-22801.

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The integration of squeeze-film dampers (SFDs) in aero-engine assemblies is a highly cost-effective means of introducing damping in an otherwise lightly damped structure. However, their deployment requires careful unbalance response calculations that take due account of the SFDs’ nonlinearity, particularly when they are unsupported by a centralising spring. Until recently, such calculations were prohibitive due to the large number of assembly modes that typically need to be considered. This problem has been overcome by the authors through the novel Impulsive Receptance Method (IRM) and the Receptance Harmonic Balance Method (RHBM), which efficiently solve the nonlinear problem in the time and frequency domains respectively. These methods have been illustrated on a realistic twin-spool engine and have been shown to be effective for both single frequency unbalance (SFU) excitation (unbalance on a single rotor) and multi-frequency unbalance (MFU) excitation (unbalance on both rotors). In the present paper, the methods are applied to a realistic three-spool engine and the aims are two fold: i) to present some preliminary results of a parametric study into a three-spool aero-engine assembly; ii) to propose a technique that makes use of both IRM and RHBM in producing the speed responses under MFU excitation (from all three rotors), with a realistic speed relation between the rotors. The latter technique is necessary since the speed ratio will vary along a realistic speed characteristic and the authors have previously solved the twin-spool MFU problem under a constant speed ratio condition. The approach used here is to approximate the speed characteristic by one in which the speed ratios are ratios of low integers, enabling the use of RHBM to finish off (to steady state) time-transient solutions obtained through IRM. The parameter study shows that the application of simple bump-spring supports to selected, otherwise unsupported, SFDs, along with slight sealing, should have a beneficial effect on the dynamic response of aero-engines with heavy rotors.
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Lacunza, Ezequiel, Maria V. Croce, Ariel Zwenger, Amada Segal-Eiras, and Martín C. Abba. "Abstract 3801: Rhomboid domain containing 2 (RHBDD2) over-expression is associated to colorectal cancer progression and drug sensitivity to 5-FU treatment." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-3801.

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