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Amaral, Marília Abrahão. "Modelo RHA - Retroalimentação em Hipermídia Adaptativa." Florianópolis, SC, 2008. http://repositorio.ufsc.br/xmlui/handle/123456789/90895.
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Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-graduação em Engenharia e Gestão do ConhecimentoMade available in DSpace on 2012-10-23T16:29:30Z (GMT). No. of bitstreams: 1 255755.pdf: 1875883 bytes, checksum: 9f0f27e2e8d0618ff0e4a59173c66d38 (MD5)
A presente pesquisa tem como objetivo propor uma extensão para modelos de referência em hipermídia adaptativa de acordo com resultados de avaliação de aprendizagem e estilos cognitivos dos aprendizes. É proposto, portanto, um modelo denominado RHA, Modelo de Retroalimentação em Hipermídia Adaptativa, que utiliza os resultados obtidos com as avaliações de aprendizagem e as definições derivadas dos estilos cognitivos dos aprendizes a fim de prover a retroalimentação dos demais modelos existentes em um hipermídia adaptativo. Na concepção do RHA foi adotada a representação por meio da UML (Unified Modeling Language) com objetivo de diminuir o risco de ambigüidade, facilitar o processo de modelagem computacional, proporcionar o reaproveitamento do modelo e otimizar a implementação do mesmo. Para apoiar a criação do RHA, foi adotado o modelo de referência Munich. Este utiliza UML e apresenta uma arquitetura que contempla módulos tradicionais dos hipermídias adaptativos, tais como: modelo de domínio, de usuário e de adaptatividade; porém, como os demais modelos de referência atuais, este não contempla a definição de itens explícitos à reutilização dos dados obtidos com as avaliações de aprendizagem. O modelo RHA foi criado como uma extensão do Munich, com concepção fundamentada em duas dimensões de estilos cognitivos estabelecidos (MESSICK, 1976), que nortearam a escolha de instrumentos de avaliação de aprendizagem destinados à modalidade ensino a distância. Os instrumentos de avaliação de aprendizagem abrangem atividades definidas de acordo com um grupo de ferramentas de comunicação e interação (síncronas e assíncronas) amplamente adotadas no ensino a distância. A modelagem do RHA envolve aspectos relativos a UML, como a criação das classes com seus atributos e métodos e os relacionamentos entre as classes já existentes no Munich. Para simular a aplicação do modelo RHA, foi definido um domínio de conhecimento relacionado à área de apoio ao ensino sobre Mercado de Capitais. Este tema se mostrou adequado, pois dada à quantidade de materiais e informações disponíveis sobre o assunto, é relevante a adoção de diferentes estilos de aprendizagem com tipos particulares de conteúdos e empregos das ferramentas de comunicação e interação, visando à avaliação de aprendizagem, para públicos distintos. Atualmente o número de cursos a distância no referido domínio de conhecimento é escasso, o que o torna ainda mais relevante para exploração.
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Schurter, Brandon T. "Regulation of RHA and NIP45 function by arginine methylation /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2005. http://wwwlib.umi.com/cr/ucsd/fullcit?p3166397.
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Khalil, Roya, and roya khalil@gmail com. "Impact of the surface chemistry of rice hull ash on the properties of its composites with polypropylene." RMIT University. Civil & Chemical Engineering, 2008. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20090219.115140.
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Rice hull ash (RHA) is a by-product of the rice industry. RHA is produced when rice hulls are incinerated, for example, when they are used to power steam engines in rice milling plants. Typically, this ash is disposed of in landfill sites, which may cause environmental problems. RHA has a naturally occurring silica content that is very high, ranging from 95 to 98%. This high silica content makes RHA a potential filler for polymer products. The aim of this project was to investigate the application of RHA as a filler in polypropylene. The study used a systematic approach to characterising the RHA physiochemical properties and comparing these to another commercially available grade of silica filler. The processing conditions for mixing RHA with Polypropylene (PP) were optimised to obtain the maximum tensile modulus value. Attempts were made to improve the interaction of RHA and PP by treating the RHA surface with silane coupling agents and adding functionalised polymers to the composite. Mechanical, rheological and morphological properties of the non-silanated, silanated and coupled composites were characterised and compared to determine their structure -property relationships. Rice hull ash (RHA) has a similar chemical structure to other silicas. Like any metals and metalloids, the surface of RHA contains -OH functional groups but these are very limited in quantity. RHA and PP composites have a wide processing window and the optimised processing conditions in a small batch mixer are 12 minutes, 60 rpm and 180°C. Addition of RHA into PP increases the modulus but decreases the tensile strength of the composites, attributed to poor compatibility between RHA and PP, as RHA is hydrophilic and PP is hydrophobic. The optimum loading of RHA is 20wt%. To improve the RHA and PP composites, 2 grades of silane and maleic anhydride grafted PP (MAPP) is used. Silane treated RHA composites have improved mechanical properties, especially tensile strength, attributed to enhanced interfacial interaction. The optimum is 1.5wt% for APS and 2.wt% for MPS in this system. The optimum MAPP concentration in this system is 3wt%. Properties of the RHA / PP composites show modest improvements compared to PP. The properties are not sufficient to make RHA a commercially attractive reinforcing filler for PP for high performance composite. It has potential for a cost reduction filler for low end application composites.
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Matkovic, Roy. "Caractérisation de l'implication de l'hélicase DHX9 (RHA) dans le cycle de multiplication du virus Chikungunya." Thesis, Montpellier, 2016. http://www.theses.fr/2016MONTT007.
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Les virus sont des parasites intracellulaires obligatoires recrutant des cofacteurs cellulaires afin de détourner les différents processus biologiques leur permettant notamment de répliquer leur génome et de former d'autres particules virales. Si des cofacteurs cellulaires de la réplication du virus Semliki Forest ont été récemment identifiés, très peu d'études ont permis de révéler des partenaires de la réplication du proche Alphavirus Chikungunya (CHIKV). Nous avons découvert, au cours de cette étude, un recrutement d'Hélicases à domaine DExD/H au niveau de sites de réplication du CHIKV. Parmi elles, DHX9 ou RNA Helicase A (RHA), grâce à ses propriétés de liaison et de modulation de structures des ARNs ou de complexes de Ribonucléoprotéines, est impliquée dans diverses fonctions depuis la transcription, la traduction, la réplication de génomes et jusqu'à la production de particules infectieuses de nombreux virus. Dans le cas du virus Chikungunya, nous avons caractérisé une fonction provirale dans la traduction de protéines non-structurales et une fonction antivirale dans la réplication du génome. Cette double fonction opposée est manipulée par le CHIKV afin d'assurer une production de protéines non-structurales composant le complexe de réplication tout en maintenant sa réplication. Ces travaux révèlent un nouveau mécanisme de régulation de la traduction d'ARN génomique de CHIKV et apportent des éléments de compréhension dans la dynamique de passage du phénomène de traduction à l'étape de réplication du génome CHIKVViruses are obligate intracellular parasites recruiting cellular cofactors to divert different biological processes enabling them to replicate their genome and to form other viral particles. If cellular cofactors of Semliki Forest virus replication have recently been identified, very few studies have revealed the replication partners of the very close Alphavirus Chikungunya (CHIKV). During this study, We have discovered recruitments of several DExD/H Box Helicases at the CHIKV replication sites. Among them, DHX9 or RNA Helicase A (RHA) through its RNA binding properties and in modulating RNA secondary structures or Ribonucleoproteins complexes, is involved in various functions from transcription, translation, replication of genomes and up to production of infectious particles of many viruses. In the case of Chikungunya virus, we have characterized a proviral function in the translation of non-structural proteins and an antiviral function in the genome replication. These opposite functions are manipulated by CHIKV to ensure production nonstructural proteins, components of the CHIKV replication complex while maintaining its replication. These works reveal a new translation regulation mechanism of CHIKV genomic RNA and bring some knowledge on the passage from the translation stage to the replication step of CHIKV genome
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Pedreschi, Luciana Erthal. "O tratamento centrado no paciente e a RHA : validação da versão brasileira do PCQ-Infertilidade." Master's thesis, FEUC, 2016. http://hdl.handle.net/10316/35221.
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Dissertação de mestrado em Gestão e Economia da Saúde, apresentada à Faculdade de Economia da Universidade de Coimbra, sob a orientação de Pedro Lopes Ferreira.Objetivo: O presente estudo teve como objetivos desenvolver a validação da versão brasileira do questionário PCQ-Infertilidade, verificar a correlação entre o itens avaliados através dos instrumentos PCQ Infertilidade e o FertiQol, e investigar as características psicométricas da versão brasileira do questionário PCQ-Infertilidade, através de um grupo de pacientes de duas clínicas especializadas no Rio de Janeiro. Metodologia: Tradução do questionário de sua língua original (inglês) para o português brasileiro por dois tradutores, retradução para inglês por um tradutor com inglês nativo, comparação dos resultados, aplicação do questionário em dois ambientes clínicos diferentes, juntamente com um outro instrumento já validado e análise dos resultados para validação do instrumento. Resultados: Total de 113 respostas de mulheres inférteis em fase de diagnóstico ou tratamentos de fertilidade no Rio de Janeiro, Brasil. A versão em português – Brasil do PCQ – Infertilidade apresentou boa consistência interna e a análise fatorial demonstrou que a amostra é ideal para a realização da análise. Pode-se verificar melhores avaliações dos pacientes na instituição que oferece o serviço mais individualizado. Conclusão: A versão em português - Brasil do PCQ-Infertilidade se mostrou válida e fidedigna. Como estratégias para melhorar a perceção dos pacientes de qualidade dos tratamentos, as clínicas devem tentar trabalhar na diminuição da ansiedade dos pacientes antes, durante e depois dos ciclos de tratamento, facilitando o contato destes com profissionais para discutirem sobre preocupações, participarem das tomadas de decisão e obter informações sobre o processo.
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Hidalgo, F., F. Hidalgo, J. Saavedra, C. Fernandez, and G. Duran. "Stabilization of clayey soil for subgrade using rice husk ash (RHA) and sugarcane bagasse ash (SCBA)." Institute of Physics Publishing, 2020. http://hdl.handle.net/10757/651798.
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This document studies the stabilization of the soil used as a subgrade, by adding locally available materials such as rice husk ash (RHA) and sugarcane bagasse ash (SCBA). These aggregates were added to the soil in substitution by weight between 5%, 7.5% and 10%. By adding these, the expansiveness is reduced while the maximum dry density increases, in addition the tendency of CBR is increasing and then tends to decrease proportionally to the addition of the aforementioned aggregates. This indicates a peak in CBR and expandability. The best result obtained from CBR was 33.75% with the 5% replacement mixtures.
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Lim, So Ra. "Da imagem à palavra : medo e ousadia em Hye Seok Rha, Tarsila do Amaral e Frida Kahlo." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2005. http://hdl.handle.net/10183/4595.
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Partindo da noção de interdisciplinaridade, este trabalho se centra na questão de gênero comparando formas diferentes do discurso narrativo, particularmente, em imagens e em textos autobiográficos produzidos por três mulheres artistas de diversas procedências, como a coreana Hye Seok Rha, a brasileira Tarsila do Amaral, e a mexicana Frida Kahlo. O trabalho investiga aspectos em comum e divergentes da linguagem utilizada pelas mulheres artistas, enquanto procura responder questões sobre a auto-representação feminina e as narrativas de cunho pessoal de autoria feminina. Sob esse ângulo, faz-se incursões teóricas em duas direções: uma relacionada com a questão dos gêneros artísticos, e a outra, com o ato de criar das mulheres como possibilidade de saírem da invisibilidade histórica a que foram submetidas durante séculos, tanto no terreno das artes plásticas quanto no da literatura. Desta forma, evidencia-se a existência de relações interdisciplinares que situam o presente trabalho no terreno da literatura comparada.
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Fritz, Sarah E. "Molecular basis of the DExH-box RNA helicase RNA helicase A (RHA/DHX9) in eukaryotic protein synthesis." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1437413252.
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Díaz, Morán Sira. "Temerosidad en ratas heterogéneas (N/NIH-HS) y romanas (RHA/RLA): estudios hormonales y de expresión génica diferencial." Doctoral thesis, Universitat Autònoma de Barcelona, 2012. http://hdl.handle.net/10803/133270.
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La presente Tesis tuvo como objetivos, estudiar la diferencia en ansiedad y otros rasgos conductuales relacionados (p. ej. miedo, susceptibilidad al estrés), a través de una batería de tests conductuales, en tres cepas o stocks de ratas diferentes (RHA-I -poco ansiosas-, RLA-I –muy ansiosas- y N/Nih-HS –ratas genéticamente heterogéneas-), y relacionar dichos perfiles conductuales/psicológicos con la expresión diferencial de genes en estructuras/circuitos cerebrales de conocida importancia en tales fenotipos (p. ej. amígdala e hipocampo).
Para alcanzar tales objetivos, se realizaron dos estudios (Estudio 1, con 128 ratas macho de los tres stocks/cepas mencionados; Estudio 2, con 114 ratas macho), que incluyeron las siguientes pruebas conductuales: Laberinto Circular Elevado (ansiedad incondicionada), test de actividad en situación de novedad (“timidez” y habituación en una “Caja nueva”), test de Campo Abierto, miedo condicionado a un contexto (respuesta de petrificación condicionada), adquisición de evitación activa en dos sentidos en la “Shuttle-box” (ansiedad debida a un conflicto de doble evitación –pasiva y activa- condicionada) y test de natación forzada (como modelo de síntomas relacionados con la depresión y los efectos de los antidepresivos). En el Estudio 1 se obtuvieron también medidas hormonales (ACTH, corticosterona y prolactina) en respuesta al estrés de exponer los animales a una ambiente nuevo y desconocido durante 20 minutos. Tras el análisis conductual y hormonal de estos dos estudios, en el Estudio 3 se llevó a cabo un análisis de expresión génica en amígdala e hipocampo (análisis de microarray y qRT-PCR) de subgrupos de ratas N/Nih-HS seleccionadas (a partir del Estudio 1) por presentar valores extremos en ansiedad (subgrupos de “Baja ansiedad” o “Alta ansiedad”). A partir de este análisis se pudo concluir que: a) las ratas N/Nih-HS presentan un perfil de comportamiento defensivo y hormonal cercano al estilo de afrontamiento pasivo característico de las ratas RLA-I; b) la heterogeneidad genética de las ratas N/Nih-HS y los resultados obtenidos en el análisis de microarray, permiten que sea un modelo animal útil para el estudio de las bases genéticas de la ansiedad y el miedo; c) los análisis de microarray han detectado expresión diferencial (entre dichos subgrupos de ratas extremas en ansiedad) de genes implicados en la regulación de funciones neuroendocrinas, como la prolactina, arginina-vasopresina y la oxitocina, que podrían modular las respuestas de ansiedad y miedo condicionado en amígdala e hipocampo. d) También se ha observado expresión diferencial (entre ambos subgrupos de ratas extremas en ansiedad) de genes reguladores de receptores sensoriales (sobretodo olfativos), genes de receptores opioides (como Oprd1 y Oprl1), genes que participan en funciones inmunológicas (como el gen H2-M9) y genes relacionados con la función tiroidea, como el gen TRH, Tg en hipocampo o el gen Duox2 en amígdala.The main aim of the present Thesis was to study differences in anxiety and related behavioral dimensions (e.g. fear, susceptibitilty to stress) in three different rat strains/stocks (i.e. the RHA-I –low anxious-, RLA-I –high anxious- and the N/Nih-HS –genetically heterogeneous- rat stock), as well as to study the associations among these behavioural profiles and differential gene expression in anxiety/fear/stress-related brain areas (i.e. amygdala and hippocampus). We conducted two studies (Study 1, using 128 male rats from those three strains/stocks; Study 2, using 114 male rats) to achieve those objectives, using a behavioural test battery which included: Elevated zero-maze test (unconditioned anxiety), novelty-induced exploratory activity (unconditioned fearfulness or “timidity” in a “Novel cage”), Open field test, context-conditioned fear (freezing), acquisition of two-way active avoidance in a shuttle box (instrumental aversive learning mediated by conditioned anxiety/fear, due to the double “passive avoidance/active avoidance” conflict) and forced swimming test (“depressive-related” symptoms: a model for the screening of antidepressant drugs). Baseline and post-stress (i.e. 20-min exposure to a novel cage) hormone measurements (ACTH, corticosterone and prolactin) were also obtained in Study 1. Following the behavioural and hormonal analyses of both studies a gene expression study (Study 3) was carried out. Thus, in Study 3, differential gene expression was assessed (through microarray analysis and qRT-PCR) in amygdala and hippocampus from subgroups of N/Nih-HS rats selected (from Study 1) according to their “low anxiety” or “high anxiety” behavioural profiles. From the analyses of the different studies we can conclude that: a) regarding their anxiety/fearfulness, passive coping style, “depressive-like” and stress-induced hormonal responses, the N/Nih-HS rats resemble the phenotype profiles of the relatively high-anxious and stress-prone RLA-I rat strain; b) the characteristic genetic heterogeneity of the N/Nih-HS rat stock, and the microarray gene expression results, point to that rat stock as an useful animal model to study the genetic basis of anxiety and fear; c) microarray analysis detected differential expression (between the above mentioned subgroups of N/Nih-HS rats with extreme low or high anxiety) of genes involved in the regulation of neuroendocrine functions, such as prolactin, arginine-vasopressin and oxytocin, which could participate in the modulation on anxiety and fear responses. d) Microarray analysis also detected differential expression of sensory receptor genes, opioid receptor genes (i.e. Oprd1 and Oprl1), immunity-related genes (like H2-M9) and thyroid regulatory genes, like TRH and Tg (hippocampus) and Duox 2 (amygdala).
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Qian, Shuiming. "Study of retrovirus and host interplay: RNA helicase A and microRNA pathway modulate viral gene expression." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1236621870.
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Fröberg, Linn, and Josefin Ravn-Holm. "A study of language development among children with hearing disabilities in pre-school." Thesis, Malmö högskola, Lärarutbildningen (LUT), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-33664.
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Syftet med studien är att få en förståelse för hur pedagoger arbetar med hörselskadade barns språkutveckling och att synliggöra pedagogernas tankar kring ämnet. En intressant aspekt är att se hur de arbetar och förhåller sig för att alla barn ska utvecklas i språket, både enskilt och i grupp. En annan betydande fråga är hur de gör och förhåller sig för att barnen ska bli trygga i det svenska språket när många av barnen har olika hörselskador på sina öron, samt olika modersmål. Studien har utgått ifrån frågeställningarna – hur förhåller sig pedagogerna till de tvåspråkiga hörselskadade barnen? Hur ser kommunikationen ut mellan hörselskadade barn? Vilka arbetsmetoder använder pedagogerna för att främja hörselskadade barns talade och tecknade språk?. För att få svar på frågeställningarna har observationer och intervjuer gjorts och detta i en form av en kvalitativ metod. För att sedan kunna analysera resultatet har relevant forskning studeras och används. Mycket tyder på att hörselskadade barn utvecklas likadant som hörande barn men att det tar längre tid och att barnen behöver tydlighet, upprepningar och olika arbetssätt för att utveckla språket. Slutsatsen visar på att pedagogernas förhållningssätt har en stor inverkan på hur barnen väljer att försöka utrycka sig, men också hur mycket tålamod, tydlighet och upprepningar som en pedagog måste ha för att kunna främja barnens språk. Kommunikationen barnen emellan visar att det kan behövas en vuxen närvarande för att barnen ska förstå istället för att missförstå.
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Martinez, Urtecho Francisco Alfredo, and Gallo Luis Julio Oyanguren. "Diseño y evaluación de concreto especial con cenizas de cáscara de arroz (RHA) para aumentar la durabilidad de las estructuras frente a la exposición al ambiente marino." Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2019. http://hdl.handle.net/10757/628220.
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El presente estudio se realizó con la finalidad de diseñar y analizar un concreto con reemplazo parcial de ceniza de cáscara de arroz (RHA) más resistente que el convencional frente a la exposición a los ambientes marinos. Esta ceniza, resulta de la combustión de cascarillas de arroz que genera una empresa encargada de elaborar pulitón en el departamento de La Libertad – Perú. La ceniza resultante de dicho proceso posee características similares a las del cemento, empezando por el alto contenido de sílice, haciéndolo apto para ser utilizado como adición. Este componente se utilizó para reemplazar en 5, 10 y 15% al cemento tipo V dentro de la mezcla de concreto con la finalidad de estudiar su reactividad puzolánica. Adicionalmente, se realizaron ensayos de resistencia a la compresión de probetas cilíndricas a los 28 días de curado en agua. Asimismo, se optó por realizar ensayos de durabilidad a los agregados grueso y fino según la Norma C88 utilizando sulfato de magnesio (MgSO4). Finalmente, se obtuvo el porcentaje de RHA más óptimo para conseguir los mejores resultados en cuanto a la resistencia a la compresión y al ataque de sulfatos.The present study was carried out in order to design and analyze a concrete with partial replacement of rice husk ash (RHA) more resistant than the conventional one against exposure to marine environments. This ash, is the result of the combustion of rice husks that generates a company responsible for making pulitón in the department of La Libertad - Peru. The ash resulting from this process has characteristics similar to those of cement, starting with the high silica content, making it suitable for use as an addition. This component was used to replace the type V cement in the concrete mix in 5, 10 and 15% in order to study its pozzolanic reactivity. Additionally, tests of compressive strength of cylindrical specimens were carried out after 28 days of curing in water. Likewise, it was decided to perform durability tests on coarse and fine aggregates according to Standard C88 using magnesium sulfate (MgSO4). Finally, the most optimal percentage of RHA was obtained to achieve the best results in terms of resistance to compression and sulfate attack.
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Soliman, Hesham. "The RhoA/Rho kinase pathway in diabetic cardiomyopathy." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/43439.
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Diabetes mellitus leads to a unique pathological entity termed diabetic cardiomyopathy, the mechanisms of which have not been fully defined. In diabetic rat hearts, RhoA expression is increased and the RhoA/ROCK pathway is activated, while ROCK inhibition acutely improves contractile function of diabetic hearts. The mechanisms underlying this improvement and those responsible for the detrimental activation of RhoA/ROCK were investigated here.
Nitric oxide (NO) has been reported to upregulate RhoA expression in smooth muscle, and previous reports showed that iNOS expression is increased in diabetic rat hearts. In the first part of this thesis, the hypothesis that in diabetic cardiomyopathy, iNOS induction is responsible for increased RhoA expression was investigated. The results demonstrate that increased NO production from iNOS induction leads to RhoA upregulation in the diabetic heart and in isolated cardiomyocytes, contributing to the RhoA/ROCK mediated contractile dysfunction by increasing the total pool of RhoA available for activation.
In diabetic rat hearts, PKCβ₂ activation induces iNOS expression, leading to increased nitrosative/oxidative stress. This suggests that PKCβ2 might positively regulate RhoA expression, although in preliminary experiments inhibition of ROCK itself reduced RhoA expression. Therefore, in the second part, the hypothesis that PKCβ₂/iNOS and RhoA/ROCK interact together to form a positive feedback loop was tested. The results show that RhoA/ROCK overactivation is sustained by a positive feedback loop that involves PKCβ₂ activation and iNOS induction. This feedback loop requires an intact actin cytoskeleton and plays a key role in elevating superoxide production in diabetic rat hearts.
In the third part, the hypothesis that ROCK inhibition augments contraction by improving Ca²⁺ signaling was tested. Inhibition of ROCK improved contractile function and abolished the diabetes-induced delayed aftercontractions in isolated cardiomyocytes, in association with an improvement in Ca²⁺ transients.
Overall, the results show that in diabetic cardiomyopathy, overactivation of RhoA/ROCK contributes to contractile dysfunction by sustaining PKCβ2 activation, iNOS induction and superoxide production via a positive feedback loop that leads to impaired intracellular Ca²⁺ homeostasis. Inhibition of ROCK disrupts the loop, resulting in decreased oxidative stress, and improved Ca²⁺ handling and cardiomyocyte contraction, suggesting that ROCK inhibition might be a novel approach in treating diabetic cardiomyopathy.
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Ormeño, Moquillaza Eduardo André, and Vicente Neptalí Eduardo Rivas. "Estudio experimental para determinar la influencia de la aplicación de Cenizas de Cáscara de Arroz (RHA) en la estabilización de una subrasante de suelo arcilloso de baja plasticidad en Chota- Cajamarca." Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2020. http://hdl.handle.net/10757/653974.
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La construcción de las obras viales han sido siempre un reto para la ingeniería, más aún en zonas donde las condiciones demográficas y el tipo de suelo no son los más convenientes para la ejecución de este tipo de proyectos. El presente estudio tiene como objetivo primordial establecer la influencia que tiene la adición de ceniza de cáscara de arroz, como estabilizante, en la sub rasante de una carretera no pavimentada conformada por un suelo arcilloso, de baja resistencia, ubicado en el caserío de Callampampa, en Chota - Cajamarca. Durante las pruebas iniciales, se determinó que la arcilla es una CL, según clasificación SUCS, y además se obtuvo un valor de CBR (California Bearing Ratio - ASTM D 1883) de 4.30% para este suelo arcilloso. La ceniza de cáscara de arroz al ser un material de desecho y contaminante para el medio ambiente se considera como una alternativa económica y, a la vez, ecológica para una estabilización de un suelo. De este modo, se realizaron diferentes ensayos de laboratorio donde se pudo determinar que el valor del CBR incrementó de 4.30% a 20.70%, al añadir una dosis de 20% de cenizas de cáscara de arroz a la muestra; logrando alcanzar su valor óptimo para ser considerado una subrasante muy buena según el “Manual de diseño de carreteras no pavimentadas de bajo volumen de tránsito” del Ministerio de Transportes y Comunicaciones. Es así, que se puede determinar y concluir que la adición de ceniza de cáscara de arroz incrementa considerablemente las diversas propiedades mecánicas del suelo, convirtiendo un suelo deficiente en uno muy bueno.The construction of road works in the world has always been a challenge for engineering, especially in areas where the conditions and types of soil are not adequate for the execution of this type of projects. The main objective of the present study is to establish the influence that has the rice husk ash (RHA) to stabilize the subgrade layer of a no pavemented road, composed of a low resistance clayey soil located in the Callampampa hamlet, in Chota - Cajamarca. During the initial tests, it was determined that the clay is a CL, according to the SUCS classification, and also a CBR (California Bearing Ratio – ASTM D 1883) value of 4.30% was obtained for this clayey soil. RHA is a waste and pollutant material for the environment, is considered an economical and, at the same time, ecological alternative for stabilizing a soil. Thus, several tests were carried out where it determined the value of CBR increased from 4.30% to 20.70%, by adding a 20% RHA dosage, achieving its optimum value to be considered a very good subgrade according to the “Design Manual for unpaved roads with low traffic volume” of the Ministry of Transport and Communications. In this way, it can be concluded that the addition of RHA considerably increases the geotechnical properties of the soil, turning a poor soil into a very good one.
Tesis
15
Hossain, Md Kamal. "Targeting Fc Receptors for More Effective Cancer Vaccines." University of Toledo Health Science Campus / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=mco1544800037742347.
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Wilson, William. "Bétons durables à base de cendres d'écorces de riz." Mémoire, Université de Sherbrooke, 2012. http://hdl.handle.net/11143/6218.
Full textAbstract:
De nos jours, le développement durable est devenu une nécessité dans l'ensemble des sphères d'activité de notre monde, et particulièrement dans le domaine du béton. En effet, le développement des sociétés passe inévitablement par l'augmentation des infrastructures; le béton est le matériau principalement utilisé et son empreinte environnementale considérable gagnerait à être diminuée. L'industrie et la recherche sont très actives à ce niveau et la science des bétons durables est en pleine expansion. Dans ce contexte, une piste de solution est l'utilisation de résidus industriels ou agricoles comme ajouts cimentaires, ce qui permet de remplacer partiellement le ciment très polluant, tout en produisant des bétons avec une meilleure durabilité. Les cendres d'écorce de riz (RHA) présentent ainsi un potentiel cimentaire similaire aux meilleurs ajouts cimentaires actuellement utilisés, mais les applications concrètes de ce nouveau matériau demeurent peu développées à ce jour. Le présent projet a donc été conçu afin d'illustrer le potentiel des RHA d'une part dans les pays industrialisés pour améliorer la durabilité des bétons hautes performances (BHP) et pour améliorer les propriétés à l'état frais des bétons autoplaçants (BAP); et d'autre part, dans les pays en développement pour démocratiser les bétons durables produits avec des technologies adaptées aux réalités locales. Une première phase réalisée avec des RHA de haute qualité (RHAI) a ainsi été consacrée aux applications en pays industrialisés. La caractérisation des RHAI a indiqué une composition de 90% de silice amorphe, des particules légèrement plus grossières que le ciment, et une microstructure très poreuse et absorbante. Afin de pallier à cette absorption d'eau, l'optimisation du type de superplastifiant a permis de déterminer que l'utilisation du Plastol 5000 ou de l'Adva 405 avec les RHAI favorise un équilibre optimal entre dispersion initiale, rétention d'affaissement et résistances mécaniques. L'optimisation du dosage en RHAI a démontré que 10 ou 15% en remplacement du ciment améliore significativement la durabilité, que les résistances mécaniques sont peu affectées par le dosage en RHAI, et que 20% (ou plus) de RHAI entraine des problèmes de maniabilité associés à une consistance rhéo-épaississante. Suite à ces optimisations, le bon potentiel des RHAI pour améliorer la durabilité des BHP a été déterminé en comparaison avec la fumée de silice (FS) et le métakaolin : le retrait endogène a été diminué par un effet de cure interne, les perméabilités aux chlorures ont été diminuées au même niveau que par la FS, et la résistance à l'écaillage a été améliorée possiblement par diminution du ressuage de surface. Finalement, l'augmentation de la viscosité par les RHAI a été utilisée avantageusement dans les BAP ou un dosage de 10% a permis d'éviter la ségrégation sans utiliser d'adjuvant modificateur de viscosité très couteux. Une deuxième phase a consisté à optimiser l'utilisation sur bétons de RHA produites avec un four à écorces de riz artisanal fabriqué selon un modèle actuellement utilisé au Burkina Faso (RHAG). Ces RHAG ont été broyées à la même granulométrie que les RHAI et des performances mécaniques similaires ont été obtenues. Une méthode de caractérisation simplifiée adaptée aux réalités du terrain a aussi été développée : elle permet d'optimiser le broyage en limitant les outils nécessaires à un tamis de 45 tm et à une balance. Un béton pour dalle a par la suite été développé à partir d'une recette typique de béton du Burkina Faso : ce béton avec RHAG a développé des résistances similaires à un contrôle, tout en permettant des économies de 23% sur le ciment. En conclusion, ce projet a réussi à illustrer le potentiel des RHA pour favoriser le développement durable dans différents types de bétons, dans différents contextes et avec des méthodes adaptées aux technologies disponibles. Suite à ce bref résumé, je vous invite fortement à poursuivre votre lecture pour en apprendre encore davantage.
17
Kullman, Nicholas Allen. "Metallurgical Characterization of Armor Alloys for the Development and Optimization of Induction Bending Procedures." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1316013868.
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Bolinger, Cheryl Giles. "Study of translation control by a RNA helicase A-responsive post-transcriptional control element in Retroviridae." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1226513076.
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Huppertz, Tilman [Verfasser]. "Charakterisierung der Rekrutierungsdomäne der kleinen Rho-GTPasen RhoA und RhoC / Tilman Huppertz." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2009. http://d-nb.info/1023697491/34.
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Bibert, Alexandre. "Les relations syndicales franco-allemandes (France, RFA, RDA) de 1945 à 1973." Thesis, Strasbourg, 2015. http://www.theses.fr/2015STRAG048.
Full textAbstract:
La Seconde Guerre mondiale a profondément perturbé les sociétés européennes. À son issue, le champ est laissé libre à des dynamiques de recomposition passant par des rapprochements ou, au contraire, par des clivages puissants. Les populations françaises et allemandes ont été affectées au premier chef par ces évolutions. Or, comme les organisations syndicales de travailleurs constituent les principales organisations de masses, elles offrent un angle d’approche particulièrement intéressant pour appréhender le dialogue développé dans un contexte marqué par la partition de l’Allemagne, par la Guerre Froide, et par la construction européenne dont l’interpénétration des économies est un des principaux aspects. Cette thèse considère, à la croisée de la question de la réconciliation et de celle d’une coopération à venir, la mise en place des échanges syndicaux entre la France et les deux Allemagnes, examine la pratique des échanges et éclaire les phénomènes de convergence et de crispationThe Second World War profoundly disrupted European societies. When the war came to an end, a dynamic of recomposition, based either on reconciliation or on sharp divisions, took hold of the continent. The French and German populations were the first to bear the brunt of these evolutions. Because of their numerous adherents, trade unions constituted the most important mass organizations of their time, and consequently offer a particularly interesting perspective on the Franco-German dialogue, especially against the backdrop of the division of Germany into two states, the Cold War, and the construction of Europe, of which economic interpenetration was a main aspect. This thesis considers, at the crossroads of a process of reconciliation and of future cooperation, the establishment and structuring of trade unions exchanges between France and Germany, examines exchange practices, and highlights adjoining convergence and tension phenomena
21
Srivastava, Kirtiman. "Pathophysiological role of RhoA/Rho-kinase under oxygen-glucose deprivation/reperfusion and hyperglycaemia." Thesis, University of Nottingham, 2013. http://eprints.nottingham.ac.uk/13533/.
Full textAbstract:
Introduction: Oxygen-glucose deprivation (OGD)±reperfusion and hyperglycaemia exacerbate the ischaemic cerebral injuries during or after a stroke. The key biochemical events associated with these pathologies include excessive cytoskeletal remodelling, modulation of tight junction proteins and the induction of oxidative stress. Recently, the overactivities of protein kinase C (PKC), RhoA/Rho-kinase, and pro-oxidant NADPH oxidase have been shown to account for the development of these events and the consequent disruption of human blood-brain barrier (BBB) integrity. Objectives: This thesis focused on the putative roles of RhoA/Rho-kinase signalling in OGD and OGD+reperfusion-evoked modulation of cytoskeletal remodelling, tight junction proteins and oxidative stress in human brain microvascular endothelial cells (HBMEC). The effects of hyperglycaemia-mediated PKC overactivities in modulating the RhoA/Rho-kinase pathway with reference to the aforementioned parameters i.e. cytoskeletal remodelling and tight junction protein expression and localisation have also been the focus of this thesis. Methods: For the OGD studies, the HBMEC were exposed to normoxia (controls), OGD (4, 20 hours) alone and followed by reperfusion (20 hours). The HBMEC-human astrocyte (HA) cocultures were established to mimic human BBB before exposing them to the experimental conditions. The integrity and function of HBMEC-HA cocultures were measured by transendothelial electrical resistance (TEER) and flux of permeability markers sodium fluorescein (NaF) and Evan’s blue-labelled albumin (EBA), respectively. For the hyperglycaemia studies, the HBMEC monolayers and the cocultures were exposed to normoglycaemia (5.5 mM D-glucose), hyperglycaemia (25 mM D-glucose), and hyperglycaemia with inhibitors of Rho-kinase, PKC, PKC-α, PKC-β, PKC-βII, PKC-δ; and the BBB integrity and function were measured by the TEER and flux studies, respectively. Fold differences in the protein expression or activity of RhoA, Rho-kinase-2, mono- and di-phosphorylated myosin light chain-2 (MLC2), total MLC2, gp91-phox (a pivotal NADPH oxidase subunit), catalase, occludin, claudin-5, zonula occludens-1 (ZO-1), β-catenin, and vinculin were either measured by in-cell or ordinary Western analyses. Results from the OGD studies: OGD compromised the barrier integrity as observed by decreases in TEER values and concomitant increases in flux of EBA and NaF across the cocultures. Transfection of HBMEC with constitutively active RhoA also decreased the TEER and increased the NaF paracellular permeability, whereas inactivation of RhoA by anti-RhoA-IgG electroporation exerted barrier protective effects. Moreover, OGD alone and after constitutively active RhoA transfection introduced stress fibres in HBMEC, which were abrogated by inactivation of RhoA and the specific inhibition of its main effector Rho-kinase by Y-27632. In addition, dramatic increases in the protein expressions of RhoA-GTP, Rho-kinase-2, gp91-phox, and antioxidant catalase were observed in HBMEC exposed to OGD+reperfusion conditions. These along with increases in the NADPH oxidase activity and total superoxide anion levels confirmed the oxidative stress in HBMEC under these experimental conditions. A marked rise in the protein expressions of claudin-5 and β-catenin observed after OGD (20 hours) alone and followed by reperfusion may represent the effects of oxidative stress on tight and adherens junction proteins stability, respectively. These results also concurred with marked decreases in TEER and concomitant increases in the flux of EBA across the in vitro models of human BBB exposed to OGD±reperfusion conditions when compared with the controls. Cotreatment with Y-27632 under OGD±reperfusion normalised the protein expressions of RhoA, Rho-kinase-2, gp91-phox, claudin-5, catalase; activities of RhoA and NADPH oxidase; and total superoxide anions levels, alongside improving the expression of occludin and the coculture integrity under the OGD±reperfusion conditions. Results from the hyperglycaemia studies: Hyperglycaemia also increased RhoA-GTP, Rho-kinase-2, mono- and di-phosphorylated MLC2 protein levels and total PKC activity. These changes were consistent with the actin stress fibre formations, ZO-1 and occludin redistribution from HBMEC periphery. Hyperglycaemia-mediated endothelial-barrier dysfunction was further characterised by reduction in TEER and elevation in flux of EBA. Glucose normalisation, RhoA neutralisation by anti-RhoA-IgG electroporation and Rho-kinase-2 inhibition by Y-27632 normalised all abovementioned protein expressions, restored actin and tight junction protein localisations and barrier integrity. Cotreatment of HBMEC with hyperglycaemia and a general PKC inhibitor namely, bisindolylmaleimide-I normalised the Rho-kinase-2, mono- and di-phosphorylated MLC2 levels. Moreover, specific inhibitors of PKC-α (Ro-32-0432), PKC-β (LY333531), PKC-βII (CGP53353) attenuated the PKC overactivity, normalised all protein expressions, restored actin localisation and improved barrier integrity. In addition, the PKC-α and PKC-β siRNA transfections mimicked the effects of the specific inhibitors and attenuated the hyperglycaemia-evoked RhoA-GTP, mono- and di-phosphorylated MLC2 protein levels and stress fibre formations. Conclusions: The RhoA/Rho-kinase overactivities compromise the endothelial-barrier integrity, in part, by modulating the cytoskeletal remodelling and inducing the NADPH oxidase-evoked oxidative stress under OGD±reperfusion pathology. Moreover, hyperglycaemia-mediated increases in PKC-α and PKC-β activities exacerbate the endothelial-barrier dysfunction by modulating RhoA/Rho-kinase signalling pathway. Summary: These findings support the hypothesis that OGD±reperfusion and hyperglycaemia perturb BBB integrity through regulation of RhoA/Rho-kinase activity and modulation of cytoskeletal reorganisation, oxidative stress and tight junction protein expressions or localisations.
22
Turner, Stephanie J. "RhoA, B, and C in cancer study of statin-induced changes in Rho signaling, and identification of isoform-specific Rho effectors /." Diss., [La Jolla] : University of California, San Diego, 2009. http://wwwlib.umi.com/cr/ucsd/fullcit?p3359512.
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Thesis (Ph. D.)--University of California, San Diego, 2009.Title from first page of PDF file (viewed July 7, 2009). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 77-106).
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Bougherara-Souidi, Nassima. "L'Algérie vue par la presse de la RFA et de la RDA : 1962-1978." Paris 8, 1987. http://www.theses.fr/1987PA080150.
Full textAbstract:
Cette thése de doctorat porte sur le dépouillement et l'etude de sept journaux : "Die Welt", "Die Frankfurter allgemeine Zeitung", "Die frankfurter Rundschau", "Der Spiegel", "Die Zeit" (RFA), "Neues Deutschland" et "Horizont" (RDA), publies de mille neuf cent soixante-deux a mille neuf cent soixante-dix-huit. Ce travail de recherche a consiste a faire ressortir une certaine image de l'algerie independante, telle qu'elle se presentait dans cette presse, a analyser et a interpreter la maniere dont l'evolution politique, economique, sociale et culturelle algerienne a ete vecue, perçue et commentée par les journalistes allemands. Nous avons, dans une premiere partie, expliqué les raisons du choix de notre sujet, expose la methode de recherche, presenté une analyse générale des événements algériens, rèdigé une présentation generale de la presse allemande, puis celle plus particuliere de la presse ouest-allemande et est-allemande, analyse les principales etapes de l'evolution des rapports algero-ouest-est-allemands et procede a une évaluation quantitative de l'information globale consacree par ces journaux a l'algerie. Au cours d'une seconde partie, nous avons applique les methodes de l'analyse de contenu : nous avons effectue la quantification de toute l'information publiee par une etude systematique et detaillee de sa frequence et de sa mise en valeurThis research thesis is a focus on the german illustration of Algeria by the west and east press from nineteen-sixty-two to nineteen-seventy eight. The research is based on the analysis of seven newspapers published in germany during that period : "Die Welt", "Die frankfurter allgemeine Zeitung", "Die frankfurter Rundschau", "Der Spiegel", "Die Zeit" (FRG), "Neues Deutschland" and "Horizont" (DRG). The purpose of the study is to evaluate the way how the political, economic, social and cultural evolution of algeria was perceived and commented in west and east german newspapers. The first part gives explanations about the choice of the matter. It presents also the methodology used for researching and analyzing the events which characterized this algerian period of development. A general presentation of the german press and that particular of west and east parts. The principal stages of the evolution of the relations between algeria and the east and west parts are analyzed. The global informations about algeria established by german newspapers is evaluated on a quantitative bases. The second part of this study is based on a content analysis. The informations are quantified thanks to the use of a systematic study of their frequency in each
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Agard, Christian. "Nouvelles approches pharmacologiques expérimentales ciblant la voie RhoA/Rho kinase dans l'hypertension artérielle pulmonaire." Nantes, 2009. http://www.theses.fr/2009NANT2091.
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L’hypertension artérielle pulmonaire (HTAP) est une maladie rare mais grave. Idiopathique, ou associée à certaines conditions, l’HTAP se caractérise par une obstruction progressive des artères pulmonaires distales. La physiopathologie de l’HTAP est complexe : dysfonction endothéliale, vasoconstriction artérielle, prolifération des cellules musculaires lisses (CML) de la média, thromboses in situ, fibrose et inflammation. Les mécanismes moléculaires de ces processus sont multiples et encore mal compris. La petite protéine G RhoA et son effecteur Rho kinase sont impliqués dans la pathogénie de l’HTAP car elles régulent les propriétés de contraction et de prolifération de la CML vasculaire. La sérotonine (5-HT), agent mitogénique et constricteur de la CML d’artère pulmonaire, et son transporteur (5-HTT) sont également impliqués dans l’HTAP. Cette thèse décrit l’intéraction entre la voie 5-HT/5-HTT et la voie RhoA/Rho kinase dans l’HTAP chez l’homme. Dans les poumons de patients avec HTAP, l’activation de RhoA/Rho kinase ainsi que la sérotonylation de RhoA, par transamination, ont été observées. In vitro, la prolifération, induite par la 5-HT, de CML d’artère pulmonaire met en jeu le 5-HTT et la voie RhoA/Rho kinase. Par ailleurs, nous avons testé la metformine, antidiabétique oral de la famille des biguanides, dans l’hypertension pulmonaire expérimentale chez le rat. En traitement préventif et curatif, la metformine a amélioré les paramètres hémodynamiques et les lésions de remodelage de l’hypertension pulmonaire. L’origine de l’effet thérapeutique est multiple : amélioration de la fonction endothéliale, inhibition de la prolifération des CML d’artère pulmonaire, inhibition de la vasoconstriction. Les effets s’expliquent au moins en partie par une inhibition de la voie RhoA/Rho kinase, possiblement médiée par l’activation de l’AMPK. Nos travaux ont contribué à l’analyse du rôle et de la régulation de la voie de signalisation RhoA/Rho kinase dans l’HTAP et ont permis d’identifier une nouvelle approche pharmacologique dans cette maladie
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Will, Laura Christine [Verfasser]. "Charakterisierung der Interaktion von p120ctn mit den Rho-GTPasen RhoA und RhoC / Laura Christine Will." Gießen : Universitätsbibliothek, 2019. http://d-nb.info/1178320073/34.
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Weber, Pierre-Frédéric. "Le Triangle RFA-RDA-Pologne (1961-1975) : guerre froide et normalisation des rapports germanos-polonais." Paris 3, 2006. http://www.theses.fr/2006PA030163.
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La normalisation germano-polonaise après la Seconde Guerre mondiale, surtout entre 1961 et 1975, s’apparente à un changement idéel à travers l’interaction dans le micro-système triangulaire formé par les deux Allemagne (RFA, RDA) et la Pologne dans le macro-système de la guerre froide. Vu le rôle actif d’acteurs non-étatiques dans ces États et à partir de plateformes transnationales (Églises, ONG), on essaie d’aller au-delà de l’histoire diplomatique traditionnelle par une évaluation de l’influence de ces acteurs sur les relations germano-polonaises depuis une position dite « nano-systèmique ». Les enjeux majeurs (parmi lesquels la ligne Oder-Neisse, la question européenne et la sécurité européenne) sont traités de façon chronologique et thématique. La « normalisation » se définit comme le résultat de modifications des identités et des intérêts des acteurs collectifs par l’interaction inter-systèmique – un modèle constructiviste applicable aux rapports germano-est-européens en généralGerman-Polish normalization after World War II and specifically between 1961 and 1975 can be seen as a mainly ideational change through interaction in the triadic microsystem formed by both German states (FRG and GDR) and Poland in the macrosystem of the Cold War. Given the active role of non-state actors in those three states as well as on transnational platforms (Churches, NGO’s), this study tries to go farther than traditional diplomatic history. Thus it also includes an appreciation of those non-state actors’ influence on German-Polish relations out of a so called “nanosystemic” position. The main issues (among which: the Oder-Neisse line, the German question and European security) are addressed both chronologically and thematically. “Normalization” is defined as the result of shifts in the corporate actors’ identities and interests through intersystemic interaction – an constructivist explanation that could be extended to other case studies of German-East-European relations
27
SECOUE, MARINETTE. "Epitaxie "par jets moleculaires sur gaas des composes du rhodium rh2 : :(a)s et rhga"." Rennes 1, 1987. http://www.theses.fr/1987REN10068.
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Deposition dans un bati d'epitaxie par jet moleculaire de rh::(2) as sur ga as. Rh::(2) as peut etre obtenu en couche mince, mais l'epitaxie n'est pas realisee selon les directions souhaitees et le compose n'est pas thermiquement stable jusqu'a 600c. Etude du diagramme ternaire rh-ga-asd afin de trouver un compose thermiquement stable. Le compose rh ga apparait stable jusqu'a 600c, il a donc ete depose sur ga as. L'accord de maille n'est cependant pas suffisamment bon pour permettre d'envisager une reepitaxie de bonne qualite du gaas sur rhga
28
Will, Laura [Verfasser]. "Charakterisierung der Interaktion von p120ctn mit den Rho-GTPasen RhoA und RhoC / Laura Christine Will." Gießen : Universitätsbibliothek, 2019. http://d-nb.info/1178320073/34.
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Fitoz, Hatice Eda. "Response Of Asymmetric Isolated Buildings Under Bi-directionalexcitations Of Near-fault Ground Motions." Master's thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614121/index.pdf.
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Isolator displacements, floor accelerations, roof displacements, base shear and
torsional moments are basic parameters that are considered in the design of
seismically isolated structures. The aim of this study is to evaluate the effects of bidirectional
earthquake excitations of near fault records on the response of base
isolated structures in terms of basic parameters mentioned above. These parameters
computed from nonlinear response history analysis (RHA) and they are compared
with the parameters computed from equivalent lateral force procedure (ELF). Effect
of asymmetry in superstructure is also examined considering mass eccentricity at
each floor level. Torsional amplifications in isolator displacements, floor
accelerations, roof displacements and base shear are compared for different level of
eccentricities. Two buildings with different story heights are used in the analyses.The building systems are modeled in structural analysis program SAP2000. The
scaling of ground motion data are taken from the study of &ldquoResponse of Isolated Structures Under Bi-directional Excitations of Near-fault ground Motions&rdquo
(Ozdemir, 2010). Each ground motion set (fault normal and fault parallel) are applied simultaneously for different range of effective damping of lead rubber bearing (LRB) and for different isolation periods.
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Stirling, Lee. "Dual Roles for Rhoa/Rho-Kinase in the Regulated Trafficking of a Voltage-Sensitive Potassium Channel." ScholarWorks @ UVM, 2009. http://scholarworks.uvm.edu/graddis/223.
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Kv1.2 is a member of the Shaker family of voltage-sensitive potassium channels and contributes to regulation of membrane excitability. The electrophysiological activity of Kv1.2 undergoes tyrosine kinase-dependent suppression in a process involving RhoA. We report that RhoA elicits suppression of Kv1.2 ionic current by modulating channel endocytosis. This occurs through two distinct pathways, one clathrin-dependent and the other cholesterol-dependent. Activation of RhoA downstream effectors Rho-kinase (ROCK) or protein kinase N (PKN) via the lysophosphatidic acid (LPA) receptor elicits clathrin-dependent Kv1.2 endocytosis and consequent attenuation of its ionic current. LPA-induced channel endocytosis is blocked by ROCK inhibition , dominant negative PKN, or by clathrin RNAi. In contrast, steady-state endocytosis of Kv1.2 in un-stimulated cells is cholesterol-dependent. Inhibition of basal ROCK with Y27632 or basal PKN with HA1077 increases steady-state surface Kv1.2. The Y27632-induced increase persists in the presence of clathrin RNAi and, in the presence of the sterol-binding agent filipin, does not elicit an additive effect. Temperature block experiments in conjunction with studies that perturb trafficking of newly synthesized proteins from the Golgi demonstrate that basal ROCK affects cholesterol-dependent trafficking by modulating the recycling of constitutively endocytosed Kv1.2 back to the plasma membrane. Both receptor-stimulated and steady-state Kv1.2 trafficking modulated by RhoA/ROCK require the activation of dynamin as well as the ROCK effector LIM kinase, indicating a key role for actin remodeling in RhoA-dependent Kv1.2 regulation.
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Krause, Sven Matthias. "Small Rho-GTPase function in myelinating cells of the vertebrate peripheral nervous system with focus on RhoA /." Zürich : ETH, 2008. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17732.
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Sadeghi-Haghighi, Nazanin [Verfasser]. "Vergleich der RDA- und REA-Werte von humanen und bovinen Zahnhartsubstanzproben mit verschiedenen Kunststoffproben / Nazanin Sadeghi-Haghighi." Ulm : Universität Ulm, 2019. http://d-nb.info/1190727307/34.
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Duart, Marcelo Adriano. "ESTUDO DA MICROESTRUTURA DO CONCRETO COM ADIÇÃO DE CINZA DE CASCA DE ARROZ RESIDUAL SEM BENEFICIAMENTO." Universidade Federal de Santa Maria, 2008. http://repositorio.ufsm.br/handle/1/7693.
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The use of mineral adition in concrete is a widely studied theme for several researchers that looking for features
like: save cement for reducing costs, durability improvement to increase mechanical strenght and use of
industrial wastes for reducing environmental pollution.
This study intend to analize the microstructure of concretes mixtures with ungrounded residual rice husk ash
addition without burn control unground (RHAN), like a partial cement substitute in conventional concrete for
reducing costs, since cement is the most expensive component, and the use RHAN avoiding dumping on
environment like a ground and water pollutant. Five different concrete mixtures proportions: 2 mixes of RHAN,
with 15% and 25% of partial cement substitution, 2 mixes of ground RHA (RHAM), with 15% and 25% of partial cement substitution, for comparison, and a control design, withouth adition. All concrete disigns were developed for 3 water/binder relationships (0,45, 0,55 e 0,65), 15 differents concrete design in total, for ages 28
and 91 days, were done. Tests like: axial compressive strenght, percentual of chemically combined water, mercury intrusion porosimetry,X-ray difraction and image analysis by scanning eletronic microscope (SEM) combined energy
dispersive spectroscopy (EDS) were done. Tests results proved that RHA use is feasible for 15% of substitution
of cement by RHAN, since concretes had values of compressive strenght few smaller than control concrete
values, at 28 days, however higher than it at 91 days.
Because of pozolanic reaction and filler efect combination, of RHAN and RHAM particles, concrete with RHA
adition had microstructural changes like: size pore refinement, total porosity reduction and calcium silicate
hydrate (C-S-H) formation through pozolanic reaction. RHA Inert particles created nucleation sites improving
hydrate crystals formation.O uso de adições minerais em concretos é um tema bastante estudado por vários pesquisadores que buscam características como: redução do consumo de cimento para redução de custos, aumento da durabilidade, aumento da resistência mecânica e aproveitamento de resíduos industriais para redução da poluição ambiental. Este estudo pretende analisar a microestrutura dos concretos dosados com adição de cinza de casca de arroz residual sem controle de queima e sem moagem (CCAN), em substituição parcial de cimento em concretos convencionais com a finalidade de redução de custos, já que o cimento é o componente mais caro do concreto e também aproveitar a CCAN evitando assim que seja descartada no ambiente como um poluente de água e solo. Foram estudados diferentes traços de concreto: 2 traços com CCAN em teores de 15 e 25% de substituição parcial de cimento, 2 traços com CCA moída (CCAM) em teores de 15 e 25% de substituição parcial de cimento, para comparação, e um traço de referência, ou seja sem substituição de cimento. Cada traço foi desenvolvido para 3 relações a/ag (0,45, 0,55 e 0,65), totalizando então 15 diferentes traços, que foram estudados para as idades de 28 e 91 dias. Foram realizados ensaios de resistência à compressão axial, teor de água quimicamente combinada, porosidade por intrusão de mercúrio, difração de raios x e análise de imagens no microscópio eletrônico de varredura (MEV) combinado com a espectrometria de dispersão de raios X (EDS). Os resultados demonstraram que a utilização da CCAN é viável para teores de 15% de substituição de cimento, já que produziu concretos com valores de resistência à compressão pouco inferiores aos valores do concreto de referência, aos 28 dias, porém superiores a este quando a idade analisada é 91 dias. Devido à combinação da reação pozolânica e efeito filler das partículas de CCAN e CCAM, os concretos com adição de CCA apresentaram modificações da microestrutura como: refinamento dos poros, redução da porosidade total, formação de silicatos de cálcio hidratados (C-S-H) a partir da reação pozolânica. Partículas inertes de CCA criaram também espaços para a nucleação aumentando a formação de cristais hidratados.
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Aurenche, Emmanuelle. "Le Vaterbuch (1971-1988) : Les écrivains de RFA, de RDA et d'Autriche et le passé nazi de leurs parents." Paris 3, 1992. http://www.theses.fr/1993PA030030.
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Pour des raisons compexes, apparait dans les annees 70-80, en rfa, en rda et en autriche, un genre specifique cree par une generation d'ecrivains nes apres l'effondrement du reich hitlerien, qui s'interroge sur le passe nazi de ses parentsFor different complex reasons a new literary genre appears in the 70-80s in frg, gdr and austria. This genre has been created by a generation of writers,who where born after the collapse of the hitlerian reich. These writers reflect the nazi past of their parents
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Gonçalves, Ana Rosária Oliveira. "Procedimentos de licenciamento de utilizações de água nas regiões hidrográficas do Sado e Mira (RH6) e do Guadiana (RH7)." Master's thesis, Universidade de Évora, 2012. http://hdl.handle.net/10174/18244.
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Actualmente a água é um valor patrimonial ecológico e social que satisfaz funções em detrimento do seu uso. Face a este modelo, é necessário efetuar uma gestão integrada de recursos naturais, onde a água desempenha um papel decisivo. O presente quadro legal e institucional português, assente na Directiva Quadro da Água, aponta claramente para uma visão de protecção e utilização sustentável das massas de águas subterrâneas e de superfície, quer sejam interiores, estuarinas ou costeiras. Nesta perspectiva, visando a melhoria contínua dos serviços prestados, apostando na modernização e simplificação administrativa para uma utilização eficiente e sustentável das águas, procede-se à organização metódica, coerente e integrada dos procedimentos de licenciamento no âmbito da utilização dos recursos hídricos para captação de águas existentes nas regiões hidrográficas do Sado e Mira (RH6) e do Guadiana (RH7), afectas à Administração da Região Hidrográfica do Alentejo, I.P. Esta estrutura de procedimentos sistematiza os trâmites associados aos procedimentos legais e técnicos, desde a formalização da instrução de pedido, à decisão e consequente emissão de título. Adoptando uma perspectiva de consistência na tramitação dos processos de licenciamento, pretende-se formalizar nesta metodologia, uma ferramenta, para utilizadores e técnicos superiores da administração pública, que possibilite maior celeridade e eficácia na apreciação processual, bem como minimizar constrangimentos ao desenvolvimento das actividades associadas e/ou dependentes de captações de água; ABSTRACT: Nowadays water is an ecological and social heritage with functions determined by its use. This model requires natural resources integrated management, where freshwater plays a decisive role. The portuguese present institutional and legislative framework, regulations that transpose the Water Framework Directive, lead to protection and sustainable use of inland surface waters, transitional waters, coastal waters and groundwater. Hence, in order to improve official services for a simpler and more modern administration, aiming efficient and sustainable use of water, this procedures establishes a coherent and methodical organization of water use permit procedures for Sado and Mira (RH6) and Guadiana (RH7) river basin districts, in the Administração de Região Hidrográfica do Alentejo, I.P. area. This methodology gathers water permit legal and technical procedures, from the request to permit issuance. This guide to both water users and permit writers will ensure consistency in procedures in order to create a faster and effective review instrument and minimize water abstraction dependent activities constraints.
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Bei, Yihua. "Implications de la voie RhoA/Rho-kinases dans la physiopathologie des atteintes vasculaires et interstitielles pulmonaires des maladies respiratoires chroniques : études humaines et expérimentales chez la souris." Thesis, Paris 5, 2013. http://www.theses.fr/2013PA05T011/document.
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La voie RhoA/Rho-kinases (ROCK) joue un rôle important dans la physiopathologie de l’hypertension pulmonaire (HTP) par son implication dans le dysfonctionnement endothélial, la constriction et le remodelage des vaisseaux pulmonaires. Selon les classifications internationales, la bronchopneumopathie chronique obstructive (BPCO) et la pneumopathie infiltrante diffuse (PID) sont deux causes fréquentes d’HTP ayant en commun plusieurs mécanismes physiopathologiques dont le dysfonctionnement endothélial, le remodelage vasculaire et la fibrose parenchymateuse. Les objectifs de ce travail étaient d’étudier le rôle de la voie RhoA/ROCK dans la physiopathologie de la BPCO et de la PID avec ou sans HTP et de préciser les anomalies moléculaires liées à la perturbation de la signalisation de cette voie dans chacune de ces situations.Le dysfonctionnement endothélial est un événement essentiel dans l’initiation et la progression de la BPCO. L’activation de la voie RhoA/ROCK dans le dysfonctionnement endothélial systémique et pulmonaire a été mise en évidence chez les tabagiques avec ou sans BPCO. Les résultats de notre première étude montrent l’existence d’une activation de la voie RhoA/ROCK au niveau des artères pulmonaires chez les patients BPCO ayant un dysfonctionnement endothélial, et une corrélation entre l’activité de la RhoA et l’expression génique et l’activité de la NO synthase endothéliale (NOS-3).L’HTP est une complication grave des PID. Nous avons montré dans notre deuxième étude l’implication de la voie RhoA/ROCK dans la réponse inflammatoire et la fibrose pulmonaire (FP) dans un modèle murin de PID induite par injection intratrachéale de bléomycine (BLM). Nous avons ensuite testé l’effet préventif du fasudil, un inhibiteur des ROCK, sur l’apparition de la FP et l’HTP expérimentales induites par la BLM. Les résultats de cette deuxième étude montrent que la FP et l’HTP sont associées à une activation de la voie RhoA/ROCK dans ce modèle murin et que le fasudil inhibe la réponse inflammatoire, la FP et l’HTP, via l’inhibition de la phosphorylation de Smad2/3 de la voie de signalisation par le TGF-β1.La FP et l’HTP représentent deux causes principales de mortalité liée à la sclérodermie systémique (ScS). Nous avons étudié le rôle de la voie RhoA/ROCK dans la physiopathologie de la fibrose cutanée et l’atteinte pulmonaire dans un modèle murin de ScS induite par injection intradermique d’acide hypochloreux (HOCl). Les résultats de cette troisième étude montrent l’association entre la fibrose cutanée induite par l’HOCl et l’activation de la voie RhoA/ROCK au niveau de la peau, et l’effet préventif du fasudil sur la fibrose cutanée et pulmonaire, en partie via l’inhibition de la phosphorylation de Smad2/3 et de l’activation des protéines ERK1/2. Ces résultats suggèrent l’implication de la voie RhoA/ROCK dans la physiopathologie de la BPCO et de la PID avec ou sans HTP. La voie RhoA/ROCK pourrait de ce fait représenter une nouvelle cible thérapeutique dans la BPCO et la PID avec ou sans HTP.Mots-clés : RhoA, Rho-kinases, fasudil, BPCO, fibrose pulmonaire, hypertension pulmonaireThe RhoA/Rho-kinases (ROCK) pathway plays a pivotal role in the pathophysiology of pulmonary hypertension (PH) as its abnormal activation leads to endothelial dysfunction, sustained vasoconstriction and pulmonary vascular remodeling. According to the international classification of PH, chronic obstructive pulmonary disease (COPD) and interstitial lung disease (ILD) represent two main causes of PH associated with chronic respiratory diseases. These two causes have in common major pathophysiological mechanisms such as endothelial dysfunction, vascular remodeling and interstitial fibrosis. The aims of the present study were to investigate the role of the RhoA/ROCK pathway in the pathophysiology of lung vascular and interstitial injuries in COPD and ILD with or without development of PH, and to study the molecular mechanisms associated with regulation of the RhoA/ROCK pathway in each of these situations.The pulmonary endothelial dysfunction is an essential event in the initiation and progression of COPD. Although the role of the RhoA/Rho-kinase pathway in pulmonary endothelial dysfunction has been demonstrated in smokers with normal lung function, little is known about its role in patients with COPD. The results of our first study demonstrated an increase in RhoA and ROCK activity in pulmonary arteries of patients with COPD, simultaneously with an altered pulmonary endothelial-dependent vasodilation. The increased RhoA activity in patients with COPD was correlated with an impairment of the gene expression and activity of endothelial NO synthase (eNOS).PH associated with pulmonary fibrosis (PF) considerably worsens prognosis of ILD. The results of our second study showed an activation of the RhoA/ROCK pathway in lung tissues of mice intoxicated by intratracheal instillation of bleomycin (BLM). BLM induced severe PF and PH in mice, associated with an increased RhoA and ROCK activity in the lung. We further demonstrated that long-term treatment with fasudil, a selective ROCK inhibitor, reduced BLM-induced lung inflammation, lung fibrosis and PH in mice, at least in part, via inhibition of Smad2/3 phosphorylation in TGF-β1 signaling.PF and PH represent two leading causes of death in patients with systemic sclerosis (SSc). In our third study, we investigated the role of the RhoA/ROCK pathway in the pathophysiology of skin fibrosis and lung injuries in a murine model of SSc induced by intradermal injection of hypochlorous acid (HOCl). We demonstrated that HOCl-induced skin fibrosis was associated with an activation of the RhoA/ROCK pathway in the fibrotic skin, and that long-term treatment with fasudil reduced both skin and lung fibrosis through inhibition of the phosphorylation of Smad2/3 and ERK1/2 in the fibrotic skin.These results suggest the implications of the RhoA/ROCK pathway in the pathophysiology of lung vascular and interstitial injuries in COPD and ILD with and without development of PH. The RhoA/ROCK pathway might be a promising therapeutic target for patients with COPD or ILD with and without PH
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Meir, Michael [Verfasser], and Nicolas [Akademischer Betreuer] Schlegel. "Bedeutung der desmosomalen Adhäsion und Rolle der Rho-GTPasen RhoA, Rac1 und Cdc42 für die Regulation der Darmbarriere / Michael Meir. Betreuer: Nicolas Schlegel." Würzburg : Universität Würzburg, 2013. http://d-nb.info/1107802563/34.
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Rodrigues, Artemis Socorro do Nascimento. "Caracterização molecular dos antigenos RhD, (RhD fraco e RhD parcial) e sua aplicação na pratica transfusional." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310418.
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Orientador: Lilian Maria de CastilhoTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: Considerando a imunogenicidade e importância clínica do antígeno RhD bem como o grande número de variantes RhD identificadas, estudos que possam esclarecer sua expressão e mecanismos moleculares envolvidos são importantes para a padronização de técnicas moleculares e sorológicas em diferentes populações. Assim foram nossos objetivos: padronizar técnicas moleculares para realização da genotipagem RHD fraco e determinar sua ocorrência na população brasileira; associar os tipos de RhD fracos encontrados com os haplótipos Rh presentes; e avaliar a aplicação da determinação do antígeno RhD na prática transfusional. Estudamos 503 amostras de DNA de doadores voluntários de sangue fenotipados como RhD ftaco. Destas amostras de DNA estudadas, 415 (82,5%) foram caracterizadas como RhD ftaco, 65 (12,9%) como RhD parcial, 15 (3%) apresentaram associações de RhD parcial e RhD ftaco e 8 (1,6%) foram RhD normal. I Os antígenos RhD fraco tipos 1, 3 e 4 foram os mais fteqüentes em nossa população. Como estes três tipos de RhD fraco não apresentam risco de aloimunização anti-D, pacientes assim classificadospodem ser transfundidos com sangue RhD-positivo. Nossos resultados demonstraram que 12,~A>das amostras fenotipadas como RhD fraco eram na verdade RhD parcial. Os antígenos RhD parciais encontrados em nosso estudo foram D~ DHMi e DVI. Quarenta (7,9%) amostras de DNA foram caracterizadas como D~ 16 (3,2%) como DHMi e 9 (1,8%) como DVI. A caracterização dos antígenos RhD parciais que reagem sorologicamente como RhD ftaco, tais como D DHMi e RhD categoria VI pode ser de grande auxilio na prevenção da aloimunização anti-D em pacientes politransfundidos e gestantes. A freqüência dos antígenos RhD parciais D~ DHMi e DVI encontrada em nossas amostras sugere um elevado risco de aloimunização ao antígeno RhD em pacientes fenotipados como RhD ftaco. - Das 503 amostras estudadas, 15 apresentaram mutações responsáveis pela expressão do antígeno RhD fraco e ao mesmo tempo mutações características de antígenos RhD parciais, ou seja, estas amostras possuíam os antígenos RhD fraco e RhD parcial associados. Estudamos quatro amostras de DNA de pacientes fenotipados como RhD :fraco que apresentavam anti-D. Nosso estudo demonstrou que a aloimunização anti-D nestes pacientes estava relacionada à presença de um antígeno RhD parcial e não a um antígeno RhD :fraco como diagnosticado sorologicamente. Duas amostras foram classificadascomo RhD parcial DAR, 1 como RhD parcial DHMi e 1 como DVI. Os resuhados demonstraram que os tipos de RhD fraco 1, 2, 3 e 4 que foram detectados à TA ou à 3'te e apresentaram grau de aglutinação superior a 1+ na AGH podem ser considerados como RhD positivo, pois não foram associados ao antígeno RhD parcial. Apesar deste trabalho ter sido o único que relacionou os tipos de RhD ftaco com o grau de aglutinação, a literatura revela que ainda não foi demonstrada aloimunização anti-D em pacientes portadores dos antígenos RhD fraco tipos 1,2 e 3. De acordo com os nossos resultados pode-se concluir que: 1. A transfusão com sangue RhD-positivo pode ser recomendada para todos os pacientes que apresentam os tipos do antígeno RhD :ftaco 1, 3 e 4 identificados por técnicas moleculares e para aqueles que apresentarem grau de aglutinação superior a 1+ na fenotipagem RhD. 2. A utilização de métodos de fenotipagem mais sensíveis em combinação com reagentes anti-D de alta afinidade é recomendada na detecção de antígenos RhD ftaco com baixa densidade antigênica em doadores de sangue; 3. Há necessidade da utilização de dois anti-soros monoc1onais (IgM e IgG) na determinação do antígeno RhD :ftacoem pacientes; 4. As genotipagens RHD, RHD ftaco e RHD parcial devem ser rea1i73dasquando os resuhados sorológicos não forem claros ou quando o paciente for politransfundido. 5. A biologia molecular associada à hemaglutinação pode aumentar consideravelmente a segurança transfusional pela mellior caracterização dos antígenos RhD em nossa população
Abstract: The purpose of this study was to characterize by molecular studies theRhD antigens (weak D and partial D) in Brazilian blood donors. DNA samples ftom 503 blood donors phenotyped as weak D were tested by two different sequence-specific primers (pCR-SSP) assays to determine the presence or absence of RHD gene (PCR-SSP intron 4 and exon 10) and to detect the common weak D types. Ofthe 503 weak D samples studied, 415 (82,5%) were identified as weak D, 65 (12,9%) as partial D, 15 (3%) showed association ofweak D and partial D and 8 (1,6%) were normal D. Weak D types 1, 3 and 4 contributed more than 85% of alI molecular weak D types. For these 3 types, D-positive transfusion can be considered safe because no immunization events have been documented yet. These findings show for the first time the frequency of weak D types in Brazilians. Molecular analysis showed that 12,9% of the weak D phenotype samples studied carried a partia! D alIele. The partial Ds found in our study were DAR, DVI and DHMi. Forty (7,9%) DNA samples were characterized as DAR, 16 (3,8%) as DHMi and 9 (1,8%) as DVI. The characterization of the partia! D antigens DAR, DHMi and DVI may avoid alIoimmunization in patients phenotyped as weak D. Fiffeteen patients showed mutations to weak D and partia! D showing that these samples had the weak D and partia! D antigens associated. We also studied 4 DNA samples of patients phenotyped as weak D who had developed anti-D. Our study showed that anti-D alIoimmunization in these patients was associated with the presence of partia! D antigens. Two samples were classified as partia!, D DAR, 1 as DHMi and 1 was DVI.AlI the weak D types identified in our study were associated with the intensity of agglutination obtained at room temperature (RT), 3'fC and AGH. The sensitivity of detecting weak D depends on the anti-D reagent and on the exact conditions of the methods. Our results showed that the weak D types 1, 2, 3 and 4 were frequently detected at RT and 3'fC and therefore could be considered as D-positive for transfusion. According to our results we could recommend the use ofmonoclonal anti-DIgM with high avidity to detect weak D antigen with low antigen density in blood donors and two monoclonals, one IgM and one IgG in combination with AGT to detect the weak D antigen in patients
Doutorado
Ciencias Basicas
Doutor em Clínica Médica
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Ghiaur, Gabriel. "The role of Rho GTPases in hematopoietic stem cell biology RhoA GTPase regulates adult HSC engraftment and Rac1 GTPases is important for embryonic HSC /." Cincinnati, Ohio : University of Cincinnati, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1204374567.
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Müller, Olga [Verfasser], and Perihan [Akademischer Betreuer] Nalbant. "Die Rolle der Rho-GTPase RhoA in der Transkriptionskontrolle der Matrix Metalloproteinase 9 (MMP9) in U-2 OS Zellen / Olga Müller ; Betreuer: Perihan Nalbant." Duisburg, 2016. http://d-nb.info/1116941910/34.
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Sissia, Julie. "Regards sur les deux Allemagnes : la place de la RFA et de la RDA dans les discours sur l’art contemporain en France, 1959-1989." Thesis, Paris, Institut d'études politiques, 2015. http://www.theses.fr/2015IEPP0043.
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Cette thèse propose d’étudier la place des deux Allemagnes, c’est-à-dire de la RFA et de la RDA, dans les discours sur l’art contemporain en France entre 1959 et 1989. Il s’agit d’aborder de manière nouvelle l’histoire de l’art en France en considérant la place qu’y occupent ces deux pays. Leur confrontation met en relief la manière dont le regard sur les deux Allemagnes nourrit les débats et les questionnements spécifiques au contexte donné de la France. L’appréhension par le biais des deux Allemagnes permet de retracer une histoire des discours sur l’art contemporain en France dans un contexte d’antagonismes idéologiques. Les enjeux esthétiques et politiques sont indissociables dans la perception de chacune des deux scènes artistiques. Ils déterminent l’écriture de l’histoire de l’art sur le vif à laquelle se livrent les protagonistes des échanges. Dans la filiation des transferts culturels et de l’histoire croisée, ainsi que des études historiographiques récentes sur l’art contemporain, cette étude met en évidence la diversité des regards sur l’art des deux Allemagnes et en souligne les points aveuglesThis PhD thesis analyses the gaze cast on divided Germany which is at stake in the narratives on contemporary art in France between 1959 and 1989. It aims at considering French art history in a new way, paying attention to the discourses developed by French art critics and historians about the GDR and the FRG. The unequal reception of those two concurrent German art scenes during the cold war reveals the political antagonisms which French discourses about art are based on. This dissertation is inspired by historical methodologies provided by cultural transfers and crossed history, as well as by recent historiographical research on contemporary art. It points out the diverse perceptions of the two Germanies and underlines their blind spots
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Miranda, Auristela Carla de. "S?ntese e impregna??o de peneiras moleculares Fe MCM-41 derivada de s?lica da casca do arroz." Universidade Federal do Rio Grande do Norte, 2011. http://repositorio.ufrn.br:8080/jspui/handle/123456789/12727.
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Previous issue date: 2011-11-28Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior
The mesoporous molecular sieves of the MCM-41 and FeMCM-41 type are considered promissory as support for metals used as catalysts in oil-based materials refine processes and as adsorbents for environmental protection proposes. In this work MCM-41 and FeMCM41 were synthesized using rice husk ash - RHA as alternative to the conventional silica source. Hydrothermal synthesis was the method chosen to prepare the materials. Pre-defined synthesis parameters were 100?C for 168 hours, later the precursor was calcinated at 550?C for 2 hours under nitrogen and air flow. The sieves containing different proportions of iron were produced by two routes: introduction of iron salt direct synthesis; and a modification post synthesis consisting in iron salt 1 % and 5% impregnation in the material followed by thermal decomposition. The molecular sieves were characterized by X ray diffraction XRD, Fourier transform infrared spectroscopy FT-IR, X ray fluorescence spectroscopy XFR, scanning electronic microscopy SEM, specific surface area using the BET method, Termogravimetry TG. The kinetic model of Flynn Wall was used with the aim of determining the apparent activation energy of the surfactant remove (CTMABr) in the MCM- 41 porous. The analysis made possible the morphology characterization, identifying the presence of hexagonal structure typical for mesoporous materials, as well as observation of the MCM41 and iron of characteristic bands.
As peneiras moleculares mesoporosas do tipo MCM-41 e FeMCM-41, s?o consideradas promissoras como suporte para metais, usadas como catalisadores em processos de refino de materiais a base de petr?leo e como adsorventes com o prop?sito de prote??o ambiental. Neste trabalho MCM-41 e FeMCM-41 foram sintetizadas utilizando a cinza da casca de arroz CCA, como alternativa para a fonte de s?lica. O m?todo escolhido foi a s?ntese hidrotermal para o preparo dos materiais, a temperatura de 100 ?C por 168 horas em estufa, posteriormente foi calcinado a 550 ?C por 2 horas sob fluxo de nitrog?nio e ar. As peneiras moleculares contendo diferentes propor??es de ferro foram produzidas por duas rotas: introdu??o do sal de ferro no gel de s?ntese e impregna??o de 1% e 5% de ferro no MCM-41 sintetizado. As peneiras moleculares foram caracterizadas por difra??o de raios X DRX, espectroscopia no infravermelho por transformada de Fourier (Fourier transform infrared spectroscopy FT-IR), espectroscopia de fluoresc?ncia de raios X FRX, microscopia eletr?nica de varredura MEV, ?rea superficial espec?fica atrav?s do m?todo BET e Termogravimetria TG. O modelo cin?tico de Flynn Wall foi utilizado com o objetivo de determinar a energia de ativa??o aparente da remo??o do surfactante (CTMABr) nos poros do MCM-41. As an?lises possibilitaram a caracteriza??o morfol?gica, identificando a presen?a da estrutura hexagonal t?pica dos materiais mesoporosos, assim como das bandas caracter?sticas do MCM41 e do ferro.
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Ard, Ryan. "Regulation of RhoA Activation and Actin Reorganization by Diacylglycerol Kinase." Thesis, Université d'Ottawa / University of Ottawa, 2012. http://hdl.handle.net/10393/22669.
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Rho GTPases are critical regulators of actin cytoskeletal dynamics. The three most well characterized Rho GTPases, Rac1, RhoA and Cdc42 share a common inhibitor, RhoGDI. It is only recently becoming clear how upstream signals cause the selective release of individual Rho GTPases from RhoGDI. For example, our laboratory showed that diacylglycerol kinase zeta (DGKz), which converts diacylglycerol (DAG) to phosphatidic acid (PA), activates PAK1-mediated RhoGDI phosphorylation on Ser-101/174, causing selective Rac1 release and activation. Phosphorylation of RhoGDI on Ser-34 by PKCa has recently been demonstrated to selectively release RhoA, promoting RhoA activation. Here, I show DGKz is required for optimal RhoA activation and RhoGDI Ser-34 phosphorylation. Both were substantially reduced in DGKz-null fibroblasts and occurred independently of DGKz activity, but required a function DGKz PDZ-binding motif. In contrast, Rac1 activation required DGKz-derived PA, but not PDZ-interactions, indicating DGKz regulates these Rho GTPases by two distinct regulatory complexes. Interestingly, RhoA bound directly to the DGKz C1A domain, the same region known to bind Rac1. By direct interactions with RhoA and PKCa, DGKz was required for the efficient co-precipitation of these proteins, suggesting it is important to assemble a signalling complex that functions as a RhoA-specific RhoGDI dissociation complex. Consequently, cells lacking DGKz exhibited decreased RhoA signalling downstream and disrupted stress fibers. Moreover, DGKz loss resulted in decreased stress fiber formation following the expression of a constitutively active RhoA mutant, suggesting it is also important for RhoA function following activation. This is consistent with the ability of DGKz to bind both active and inactive RhoA conformations. Collectively, these findings suggest DGKz is central to two distinct Rho GTPase activation complexes, each having different requirements for DGKz activity and PDZ interactions, and might regulate the balance of Rac1 and RhoA activity during dynamic changes to the actin cytoskeleton.
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Axelsson, Lena. "Karakterisering av blodgruppsgenen RHD hos patienter med svagt RhD-antigenuttryck." Thesis, Malmö högskola, Fakulteten för hälsa och samhälle (HS), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-24168.
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Rh-blodgruppssystemet är mycket komplext med 54 blodgruppsantigen som kodas av två nära varandra belägna gener på kromosom 1 – RHD och RHCE. RHD-genen kodar för RhD-proteinet, ett membranbundet protein på erytrocyter vars antigen utgör de kliniskt viktigaste och mest immunogena efter ABOsystemets, och som kan ge upphov till transfusionskomplikationer och hemolytisk sjukdom hos foster och nyfödda. Vissa individer har varianter av RhD-protein som uttrycks svagare än normalt (”svaga D”), eller där vissa epitoper saknas (”partiella D”), och för vilka serologiska metoder inte kan ge enhetliga resultat. Detta orsakar problem vid blodtransfusion, graviditet och bloddonation, och leder ofta till användning av det redan knappa lagret av RhD-negativa blodenheter för att skydda patienten. I detta projekt har åtta prover med svaga RhD-antigenuttryck sekvenserats med avseende på RHD-genen i syfte att fastställa individernas RhDfenotyp. I sex av proverna hittades sex nukleotidpolymorfismer och två deletioner, som alla är sällsynta men dock är kända sedan tidigare. I två prover kunde inga mutationer i exon eller intilliggande intron påvisas som förklaring till de svaga uttrycken av RhD hos dessa individer.The Rh blood group system is very complex with 54 blood group antigens encoded by two adjacent genes on chromosome 1 – RHD and RHCE. The RHD gene encodes the RhD protein, a membrane bound protein on erythrocytes whose antigens are the most clinically important and immunogenic after those of the ABO system, and which can result in transfusion complications and haemolytic disease of the fetus and newborn. Some individuals have variants of the RhD proteins that are expressed more weakly than normal (“weak D”), or have some of the epitopes missing (“partial D”), and for which serological methods cannot give a uniform result. This provides a problem in blood transfusion, pregnancy, and blood donation, and often results in the use of the already sparse supply of RhDnegative blood units for the safety of the patient. In this project, eight samples with weak RhD antigen expression have been sequenced with regard to the RHD gene in order to determine the RhD phenotype of the individuals. In six of the samples, six single nucleotide polymorphisms and two deletions were found, all of which are rare but are previously known. For two of the samples, no mutations in exons or adjacent introns could be detected to explain the weak expression of RhD in those individuals.
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Fauré, Julien. "Régulation des GTPases de la famille RHO par RHO-GDI." Université Joseph Fourier (Grenoble), 1999. http://www.theses.fr/1999GRE10006.
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Les membres de la famille RHO de GTPpases monomériques sont des interrupteurs moléculaires impliqués dans la transduction de nombreux signaux aboutissant notamment à la motilité cellulaire et à la transcription de gènes. Notre travail a porté sur le complexe formé entre la protéine G RHO et RHO-GDI, son régulateur naturel. Les deux protéines sont associées dans le cytosol mais il est nécessaire qu'elles soient séparées pour que RHO puisse exercer sa fonction. Nous nous sommes intéressés aux stimuli permettant d'activer leur protéine G à partir de son complexe avec RHO-GDI. Nous avons d'abord mis au point dans un système d'expression eucaryote la production de complexes formés in vivo entre les GTPases de la famille RHO et RHO-GDI. La purification de ces complexes a abouti à la production de cristaux et devrait permettre l'étude de leurs structures tridimensionnelles. Nous avons utilisé ces complexes pour étudier l'effet des phosphoïnositides sur l'état d'association de RHO avec RHO-GDI. Nous avons montré que ces lipides induisaient une conformation pré-activée du complexe, sans toutefois dissocier les deux partenaires. La méthode double-hybride nous a permis de cloner par interaction avec RHO-GDI l'ensemble des membres de la famille RHO ainsi que plusieurs régulateurs potentiels. Cette methode a ensuite servi à étudier les zones de RHO susceptibles d'interagir avec RHO-GDI grâce à la production de mutants de RHOA. L'interaction entre la GTPase RHO et RHO-GDI met en oeuvre un résidu isoprène lié à l'extrémité c-terminale de RHO. Les mutants de RHOA ne portant pas ce résidu sont cependant toujours capables d'interagir avec RHO-GDI, vraissemblablement grâce à la zone d'insertion de RHO. Enfin, une technique d'overlay a été utilisée pour mettre en évidence des partenaires membranaires du complexe RHO/RHO-GDI activé par les phosphoïnositides. Une protéine de 32kda, lâchement attachée à la membrane, a ainsi été isolée comme régulateur potentiel du complexe RHO/RHO-GDI.
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Pellizzari, Gabriele <1992>. "RCA in Europa; RCA con premi basati sull’entità del sinistro." Master's Degree Thesis, Università Ca' Foscari Venezia, 2017. http://hdl.handle.net/10579/11729.
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Guérin, Patrice. "Étude des voies de signalisation dans le phénomène de resténose intra-stent : implication de la protéine G monomérique RhoA et de son effecteur Rho-kinase." Nantes, 2005. http://www.theses.fr/2005NANT2105.
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La resténose intra-stent est une nouvelle pathologie induite par l'implantation d'une endoprothèse (stent). Elle résulte d'une prolifération des cellules musculaires lisses (CML), une migration et une production excessive de matrice extracellulaire. Le but de ce travail est d'analyser (i) les conséquences histologiques de l'implantation du stent dans un modèle de culture d'artère mammaires "ex-vivo" ; (ii) l'activité de RhoA, régulateur majeur de la prolifération et la migration des CML, après implantation du stent et de déterminer son rôle dans la formation de la néointima ; (iii) l'impact du mode d'implantation du stent sur l'épaississement néointimal dans le segment stenté et dans la zone bordante. Notre étude montre que le modèle d'artères mammaires humaines est fiable pour l'étude des conséquences histologiques de l'implantation d'un stent. Le stent induit une activation de RhoA, et l'effet inhibiteur de la rapamycine sur l'expression de RhoA a un rôle clé dans son effet limitant de la resténose. Par l'utilisation de stent actif libérant la rapamycine, nous démontrons l'intérêt de l'implantation directe du stent pour limiter l'effet de bordIn-stent restenosis is a novel pathobiologic process resulting from vascular smooth muscle cell (VSMC) proliferation, migration and excessive matrix production. The aims of this study were to analyze (i) the histological consequences of the healing process following stent implantation in an "ex-vivo" model of human internal mammary arteries; (ii) the activity of RhoA, a major regulator of VSMC proliferation and migration, after stenting and to determine its role in the neointimal formation; (iii) the influence of the stent insertion procedure on neointimal thickening in the stented segment of the artery and at the stent edge. Our study shows that the artery culture model can provide valuable information regarding histological consequences and healing process after balloon dilatation and stent implantation. Stent implantation induces maintained RhoA activation and inhibition of Rho-kinase reduces neointimal thickening. Rapamycin (sirolimus) decreases RhoA expression and this effect plays a key role in its antirestenotic action. Finally, by the use of sirolimus eluting stent, we show the benefit of direct stenting to limit the edge effect
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Lazarus, Jens. "RDA und GND." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2010. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-39043.
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Zur Zukunft der Katalogisierung werden zur Zeit zwei große Themenkomplexe diskutiert. Einmal der Aufbau einer Gemeinsamen Normdatei (GND) und die Einführung eines neuen Regelwerks, „Resource Description and Access (RDA)“. Das Regelwerk soll die bislang geltenden RAK ablösen und nicht nur für Bibliotheken, sondern auch für Archive und Museen zur Verfügung stehen. Da die verschiedenen Normdateien auf unterschiedlichen Datenmodellen und Formaten basieren, wird an einer Zusammenführung der Normdatenbanken in eine einheitliche Normdatei gearbeitet.
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Bucic, Ida. "Pollard's rho method." Thesis, Linnéuniversitetet, Institutionen för matematik (MA), 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-85886.
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In this work we are going to investigate a factorization method that was invented by John Pollard. It makes possible to factorize medium large integers into a product of prime numbers. We will run a C++ program and test how do different parameters affect the results. There will be a connection drawn between the Pollard's rho method, the Birthday paradox and the Floyd's cycle finding algorithm. In results we will find a polynomial function that has the best effectiveness and performance for Pollard's rho method.
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Vanhoefer, Pit. "Study of B0->rho rho decays with the belle experiment." Diss., Ludwig-Maximilians-Universität München, 2015. http://nbn-resolving.de/urn:nbn:de:bvb:19-183537.
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