Relevant bibliographies by topics / RFLP

Academic literature on the topic 'RFLP'

Author: Grafiati

Published: 4 June 2021

Last updated: 10 March 2023

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Journal articles on the topic "RFLP"

Trevisan, Giovani, Aditi Sharma, Phillip Gauger, Karen M. Harmon, Jianqiang Zhang, Rodger Main, Michael Zeller, Leticia C. M. Linhares, and Daniel C. L. Linhares. "PRRSV2 genetic diversity defined by RFLP patterns in the United States from 2007 to 2019." Journal of Veterinary Diagnostic Investigation 33, no. 5 (June 28, 2021): 920–31. http://dx.doi.org/10.1177/10406387211027221.

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The genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) increases over time. In 1998, restriction-fragment length polymorphism (RFLP) pattern analysis was introduced to differentiate PRRSV wild-type strains from VR2332, a reference strain from which a commercial vaccine (Ingelvac PRRS MLV) was derived. We have characterized here the PRRSV genetic diversity within selected RFLP families over time and U.S. geographic space, using available ISU-VDL data from 2007 to 2019. The 40,454 ORF5 sequences recovered corresponded to 228 distinct RFLPs. Four RFLPs [2-5-2 (21.2%), 1-7-4 (15.6%), 1-4-4 (11.8%), and 1-8-4 (9.9%)] represented 58.5% of all ORF5 sequences and were used for cluster analysis. Over time, there was increased detection of RFLPs 2-5-2, 1-7-4, 1-3-4, 1-3-2, and 1-12-4; decreased detection of 1-4-2, 1-18-4, 1-18-2, and 1-2-2; and different detection trends for 1-8-4, 1-4-4, 1-26-1, 1-22-2, and 1-2-4. An over-time cluster analysis revealed a single cluster for RFLP 2-5-2, supporting that sequences within RFLP 2-5-2 are still relatively conserved. For 1-7-4, 1-4-4, and 1-8-4, there were multiple clusters. State-wise cluster analysis demonstrated 4 main clusters for RFLP 1-7-4 and 1-8-4, and 6 for RFLP 1-4-4. For the other RFLPs, there was a significant genetic difference within them, particularly between states. RFLP typing is limited in its ability to discriminate among different strains of PRRSV. Understanding the magnitude of genetic divergence within RFLPs helps develop PRRSV regional control programs, placement, herd immunization strategies, and design of appropriate animal movements across borders to minimize the risk of PRRSV transmission.

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Young, Nevin Dale. "Restriction Fragment Length Polymorphisms (RFLPS) and Crop Improvement." Experimental Agriculture 28, no. 4 (October 1992): 385–98. http://dx.doi.org/10.1017/s0014479700020093.

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SUMMARYRestriction fragment length polymorphisms (RFLPs) are genetic markers based on cloned molecules of DNA. Using RFLPs, scientists can construct high density genetic linkage maps and locate economically important genes. Once a gene for a valuable trait has been mapped with RFLPs, desirable genotypes can be selected using RFLPs rather than scoring for the trait itself. This is important when a trait is recessive, difficult to score, or obscured by other characters. RFLPs are particularly valuable in mapping genes controlling complex polygenic characters, including those fundamental to crop improvement. The first RFLP maps were developed in crop species primarily important in temperate countries. Now RFLP maps for major tropical and sub-tropical crop species are also being developed.

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Meagher, R. B., M. D. McLean, and J. Arnold. "Recombination within a subclass of restriction fragment length polymorphisms may help link classical and molecular genetics." Genetics 120, no. 3 (November 1, 1988): 809–18. http://dx.doi.org/10.1093/genetics/120.3.809.

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Abstract Restriction fragment length polymorphisms (RFLPs) are being used to construct complete linkage maps for many eukaryotic genomes. These RFLP maps can be used to predict the inheritance of important phenotypic loci and will assist in the molecular cloning of linked gene(s) which affect phenotypes of scientific, medical and agronomic importance. However, genetic linkage implies very little about the actual physical distances between loci. An assay is described which uses genetic recombinants to measure physical distance from a DNA probe to linked phenotypic loci. We have defined the subset of all RFLPs which have polymorphic restriction sites at both ends as class II RFLPs. The frequency of class II RFLPs is computed as a function of sequence divergence and total RFLP frequency for highly divergent genomes. Useful frequencies exist between organisms which differ by more than 7% in DNA sequence. Recombination within class II RFLPs will produce fragments of novel sizes which can be assayed by pulsed field electrophoresis to estimate physical distance in kilobase pairs between linked RFLP and phenotypic loci. This proposed assay should have particular applications to crop plants where highly divergent and polymorphic species are often genetically compatible and thus, where class II RFLPs will be most frequent.

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Gedil, Melaku Ayele, Crispin Wye, Simon Berry, Bart Segers, Johan Peleman, Richard Jones, Alberto Leon, Mary B. Slabaugh, and Steven J. Knapp. "An integrated restriction fragment length polymorphism - amplified fragment length polymorphism linkage map for cultivated sunflower." Genome 44, no. 2 (April 1, 2001): 213–21. http://dx.doi.org/10.1139/g00-111.

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Restriction fragment length polymorphism (RFLP) maps have been constructed for cultivated sunflower (Helianthus annuus L.) using three independent sets of RFLP probes. The aim of this research was to integrate RFLP markers from two sets with RFLP markers for resistance gene candidate (RGC) and amplified fragment length polymorphism (AFLP) markers. Genomic DNA samples of HA370 and HA372, the parents of the F2 population used to build the map, were screened for AFLPs using 42 primer combinations and RFLPs using 136 cDNA probes (RFLP analyses were performed on DNA digested with EcoRI, HindIII, EcoRV, or DraI). The AFLP primers produced 446 polymorphic and 1101 monomorphic bands between HA370 and HA372. The integrated map was built by genotyping 296 AFLP and 104 RFLP markers on 180 HA370 × HA372 F2 progeny (the AFLP marker assays were performed using 18 primer combinations). The HA370 × HA372 map comprised 17 linkage groups, presumably corresponding to the 17 haploid chromosomes of sunflower, had a mean density of 3.3 cM, and was 1326 cM long. Six RGC RFLP loci were polymorphic and mapped to three linkage groups (LG8, LG13, and LG15). AFLP markers were densely clustered on several linkage groups, and presumably reside in centromeric regions where recombination is reduced and the ratio of genetic to physical distance is low. Strategies for targeting markers to euchromatic DNA need to be tested in sunflower. The HA370 × HA372 map integrated 14 of 17 linkage groups from two independent RFLP maps. Three linkage groups were devoid of RFLP markers from one of the two maps.Key words: amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), Helianthus, sunflower, genetic map.

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Deynze, A. E. Van, B. S. Landry, and K. P. Pauls. "The identification of restriction fragment length polymorphisms linked to seed colour genes in Brassica napus." Genome 38, no. 3 (June 1, 1995): 534–42. http://dx.doi.org/10.1139/g95-069.

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Restriction fragment length polymorphisms (RFLPs) linked to genes controlling seed colour were identified in rapeseed (Brassica napus). The efficiency of the RFLP analysis was enhanced by utilizing bulked segregant analysis, DNA clones that had previously been used to construct a RFLP map of B. napus, and a doubled-haploid (DH) population segregating for seed colour. Markers for two of the three seed colour genes segregating in the DH population were identified on the basis of χ2 analyses of marker distributions among visually classified black-, brown-, and yellow-seeded DH lines as well as ANOVA and quantitative trait locus analysis of light-reflectance measurements from seeds of the DH lines. The RFLP markers linked to seed colour that were identified in the present study will allow breeding strategies based on genotype selection to be developed for seed colour in rapeseed.Key words: RFLP markers, seed colour genes, rapeseed.

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Seldin, M. F., H. C. Morse, J. P. Reeves, C. L. Scribner, R. C. LeBoeuf, and A. D. Steinberg. "Genetic analysis of autoimmune gld mice. I. Identification of a restriction fragment length polymorphism closely linked to the gld mutation within a conserved linkage group." Journal of Experimental Medicine 167, no. 2 (February 1, 1988): 688–93. http://dx.doi.org/10.1084/jem.167.2.688.

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A linkage map of distal mouse chromosome 1 was generated using restriction fragment length polymorphism (RFLP) analysis of DNA prepared from 95 [C3H-gld/gld X Mus spretus)F1 X C3H-gld/gld] backcross mice. The gene order was: (centromere) C4bp, Ren-1,2, Ly-5, [At-3/gld], Apoa-2/Ly-17, Spna-1 (telomere). All mice expressing the phenotype of gld homozygotes were homozygous for the At-3 RFLP characteristic of C3H mice and none of the mice heterozygous for At-3 RFLPs had characteristics of gld homozygotes, demonstrating close linkage between these genes. The identification of an RFLP closely linked to the gld gene provides a starting point for the identification of a genetic defect that results in abnormal T cells and autoimmune disease.

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Jena, K. K., G. S. Khush, and G. Kochert. "Comparative RFLP mapping of a wild rice, Oryza officinalis, and cultivated rice, O. sativa." Genome 37, no. 3 (June 1, 1994): 382–89. http://dx.doi.org/10.1139/g94-054.

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A comparative RFLP map was constructed in a wild rice, Oryza officinalis, by using 139 genomic and cDNA probes that had been used previously to map RFLPs in O. sativa. Nine of the 12 chromosomes of O. officinalis were highly homosequential to those of O. sativa. A major rearrangement of gene order was detected in chromosome 1 and small inversions were found in chromosomes 3 and 11. Fourteen translocated RFLP markers were found, and chromosome 11 contained a high frequency of such translocated segments. Results were consistent with meiotic and trisomie analysis, which suggested that the genomes of O. officinalis and O. sativa were similar. Applications of comparative maps in plant breeding and gene cloning are discussed.Key words: Oryza, rice, wild rice, RFLP, genetic map.

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Landry, Benoit S., Nathalie Hubert, René Crete, Morgan S. Chang, Steven E. Lincoln, and Takeomi Etoh. "A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin)." Genome 35, no. 3 (June 1, 1992): 409–20. http://dx.doi.org/10.1139/g92-061.

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F2 segregation analyses of DNA restriction fragment length polymorphisms (RFLPs) detected between a cabbage line (No. 86-16-5) resistant to race 2 of Plasmodiophora brassicae (Woronin), the fungus responsible for clubroot disease, and a rapid cycling line (CrGC No. 85) was used to construct a detailed genetic map of Brassica oleracea. RFLP markers were random and seedling-specific cDNA clones. The 201 loci so far mapped in B. oleracea covered 1112 cM. They are assembled into nine major linkage groups and four small linkage groups. Twelve loci were found unlinked to any other markers. Twenty-one loci were detected with the 18 seedling-specific cDNAs. Two dominant QTLs for resistance to race 2 of the clubroot disease causal agent were also identified. Leaf morphology and biennial flowering appeared to segregate as single Mendelian traits, but only leaf morphology could be linked to other markers. This RFLP study in B. oleracea is providing additional information on genome organization and complements current RFLP mapping effort in B. napus.Key words: genetic mapping, Brassica oleracea, Plasmodiophora brassicae, breeding, clubroot resistance, DNA markers, RFLP.

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Garcia, G. M., H. T. Stalker, and G. Kochert. "Introgression analysis of an interspecific hybrid population in peanuts (Arachis hypogaea L.) using RFLP and RAPD markers." Genome 38, no. 1 (February 1, 1995): 166–76. http://dx.doi.org/10.1139/g95-021.

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Forty-six introgression lines (F10C9) from a cross between Arachis hypogaea L. (2n = 4x = 40) and A. cardenasii Krapov. &W.C. Gregory (2n = 2x = 20) were analyzed for the introgression of A. cardenasii chromosome segments. Seventy-three RFLP probes and 70 RAPD primers, expressing from one to four A. cardenasii-specific bands, were used to evaluate the set of introgression lines. Thirty-four RFLP probes and 45 RAPD primers identified putative A. cardenasii introgressed chromosome segments in one or more lines. Introgressed segments were detected by RFLP analysis in 10 of the 11 linkage groups; the smallest introgressed fragments were detected by single RFLP markers and the largest were detected by three or four adjacent markers and represented introgressed segments of 30–40 cM. Similar results were obtained with RAPD markers, although markers detecting introgressed fragments could not be placed on the peanut linkage map. Introgression into both A. hypogaea genomes was detected and its implication in breeding for disease resistance is discussed.Key words: peanut, Arachis hypogaea, Arachis cardenasii, RFLPs, RAPDs, introgression, reciprocal recombination, translocation, alien gene transfer, wide cross.

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Kennard, Wayne, Arian Dijkhuizen, Michael Havey, and Jack Staub. "PROGRESS TOWARD DEVELOPMENT OF AN RFLP MAP FOR CUCUMBER." HortScience 25, no. 9 (September 1990): 1159a—1159. http://dx.doi.org/10.21273/hortsci.25.9.1159a.

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The analysis of genetic linkage in cucumber (Cucumis sativus has primarily involved morphological and disease resistance markers. Linkage analysis in cucumber would benefit from more markers. Restriction fragment length polymorphisms (RFLPs) can occur in relatively large numbers within a single segregating family. Research is presently underway to construct an RFLP map of cucumber. Pst I partial genomic and cDNA libraries of cucumber have been constructed as sources of probes for RFLP analysis. Cucumber DNA from 16 accessions of cucumber and one accession of C. sativus var. hardwickii were digested with either of two restriction enzymes (EcoR I and Hind III). This germplasm allows for the assessment of the variability for RFLPs in cucumber and will provide the parents for map construction.

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Dissertations / Theses on the topic "RFLP"

Šuranská, Hana. "Identifikace vinných kvasinek metodou PCR-RFLP." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216494.

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This thesis deals with identification of the wine yeasts by applying the PCR-RFLP method. The identification and characteristic of the yeasts has gone through substantial changes in recent years. There have been introduced new methods of taxonomic classifying based on the molecular methods, which are oriented to easy and fast identification. One of these methods is the PCR-RFLP method. The amplification of the 5•8S-ITS rDNA sequence by the polymerase chain reaction with use of the primers ITS1 and ITS4 leads to the amplification of the specific sequence of DNA. Such multiplied DNA is after repurifying by the ethanol and drying submitted to the restriction analysis. With use of the restriction endonuklases DNA is chopped into the specific segments typical for the particular genus. The chopped fragments can be separated in the electric field in the agarose gel and subsequently evaluated. In this thesis together 63 type yeasts were used. These yeasts were analysed by applying of the seven restriction endonuklases – HaeIII, HhaI, HinfI, HpaII, TaqI, AluI a MseI. The final image of type yeasts splitting was compared to the results of splitting of already identified wine yeasts and these yeasts were subsequently taxonomically classified. Evaluation of genetic similarity was conducted by program BioNumerics and as the results the dendrograms that were created with use of Jaccard‘s coefficients are obtained.

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Olivová, Radana. "Optimalizace metody PCR-RFLP pro taxonomické zařazení kvasinek." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216560.

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This thesis deal with optimalization method PCR – RFLP for taxonomy enlistment of yeasts. Conventional identification methods for yeasts are time-consuming. Molecular biological method based on PCR are instrumental towards fast and precise identification as compared to conventional phenotypic methods. In this thesis molecular biological method PCR – RFLP was used for identification and enlistment of yeasts. This metod follow repeating spacers of ribozomal DNA of yeast, characteristic for each species and strain. By the help of PCR were amplified specific partitions of DNA. These fragments of DNA were split by restriction endonucleases and identified by horizontal electroforesis. In background of this thesis there are information about yeasts, their taxonomy and molecular biological methods.

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Wolf, Markus. "Markergestützte Vererbungsanalyse der Pollenfertilitätsrestauration bei Winterroggen (Secale cereale L.)." [S.l. : s.n.], 2001. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB9818604.

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Cavell, Jane Sarah. "Cytogenetic and RFLP analyses of somaclonal variation in Nicotiana tabacum." Thesis, University of Bath, 1989. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328517.

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Datta, Krishna. "Wide hybridisation and isozyme, RAPD and RFLP markers of #Corchorus' species." Thesis, Imperial College London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283339.

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Dreyer, Felix. "Chromosomale Lokalisation von Restorergenen argentinischer und iranischer Herkunft in F2-Populationen von Winterroggen /." Aachen : Shaker, 2000. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=009029454&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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SANTOS, Lorena Cristina. "Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110." Universidade Federal de Goiás, 2008. http://repositorio.bc.ufg.br/tede/handle/tde/1826.

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Made available in DSpace on 2014-07-29T15:30:41Z (GMT). No. of bitstreams: 1 Dissertacao Lorena Cristina.pdf: 430117 bytes, checksum: 09642b3a4726659137a90dcbf634ba90 (MD5) Previous issue date: 2008-02-29
Tuberculosis is a serious public health problem all over the world. It has been demonstrated that different M. tuberculosis strains, characterized by the RFLP-IS6110 standard technique, have different virulence properties and antibiotic resistance. The aim of this study was to characterize M. tuberculosis strains isolated from patients attending two state reference hospitals of Goiânia-Goiás, using the RFLP-IS6110 technique. Positive cultures of M. tuberculosis sampled and isolated from January 2006 to June 2007 had their DNA extracted. A total of 142 viable DNA samples were analyzed, of which 126 samples presented an RFLP-IS6110 profile. Similarities comparisons between samples, as well as with literature reported profiles, were done with Bionumerics software (version 4.0). Forty three percent of the samples could be grouped in 24 clusters, when analyzed by the RFLP method, suggesting recent transmission among individuals belonging to the same cluster, however those patients did not present any epidemiological relationship, suggesting possible casual transmission between members of the same cluster. When compared with strain profile of the MDR-TB, three samples had grouped in the clade formed by families virulent. This study strengthens the importance of determining transmission routes not only within the state of Goiás but in the entire country, since there is the possibility of resistant strains transmissions.
A tuberculose é um grave problema de saúde pública em todo o mundo. Foi demonstrado que diferentes cepas de M. tuberculosis, caracterizadas pela técnica padrão mundial, RFLP-IS6110, possuem diferentes graus de virulência e resistência a antibióticos. O objetivo deste trabalho foi caracterizar as cepas de M. tuberculosis isoladas de pacientes atendidos em dois serviços de referência na cidade de Goiânia-Goiás no período de janeiro de 2006 a junho de 2007, utilizando a técnica RFLP-IS6110. Foram processadas para cultura 175 amostras de escarro com baciloscopias positivas e um total de 142 culturas positivas para M. tuberculosis tiveram seus DNA extraídos, estes foram posteriormente submetidos a técnica de southern blotting para obtenção de seus perfis de bandas de RFLP-IS6110. Destas, 126 amostras apresentaram um perfil de RFLP-IS6110 de fácil comparação de similaridade no programa BioNumerics (versão 4.0). As amostras analisadas pelo RFLP-IS6110 permitiram o agrupamento de 43% das amostras em 24 clusters, sugerindo transmissão recente entre indivíduos agrupados, contudo estes pacientes não apresentaram nenhuma relação epidemiológica, sugerindo possíveis transmissões casuais entre membros de um mesmo cluster. Quando comparamos as amostras com linhagens MDR descritas na literatura, três se agruparam no clado formado pelas famílias virulentas. Este estudo fortaleceu a importância de se traçar cadeias de transmissões bem como a necessidade de se manter um controle de imigrantes e emigrantes visto que há a possibilidade de disseminação de cepas resistentes.

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Ip, Ka-fai. "Molecular epidemiological study of mycobacterium tuberculosis using IS6110-RFLP and MIRU typing /." View the Table of Contents & Abstract, 2005. http://sunzi.lib.hku.hk/hkuto/record/B31495473.

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Ziebell, Kim. "Evaluation of PCR and PCR-RFLP protocols to identify the Shiga toxins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0026/MQ51107.pdf.

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Ip, Ka-fai, and 葉嘉輝. "Molecular epidemiological study of mycobacterium tuberculosis using IS6110-RFLP and MIRU typing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B45010092.

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Books on the topic "RFLP"

Ramsden, Simon Christopher. The development of RFLP markers in sugar beet. Birmingham: Universityof Birmingham, 1991.

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Sabir, Adel Abdul-Qadir. Isozyme and RFLP analysis of somaclones of cultivated beets. Birmingham: University of Birmingham, 1990.

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Hering, Olaf. Charakterisierung und Differenzierung bei Fusarium Link mittels RAPD und ITS-RFLP. Berlin: Parey, 1997.

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Office, General Accounting. PBGC's premium accounting system RFP. Washington, D.C: The Office, 1992.

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Office, General Accounting. PBGC's premium accounting system RFP. Washington, D.C: The Office, 1992.

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Elliott, Wiley, ed. RFL, Reginald F. Lewis: A tribute. New York: Bookmark Publishing Corp., 1994.

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San Francisco (Calif.). Dept. of Public Health. Tobacco Free Project. Important request for proposal notice: [RFP 022-98, Fiscal sponsor for a community capacity building center : RFP 023-98, Media services : RFP 024-98, Evaluation services]. San Francisco, CA: Dept. of Public Health, 1999.

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Gibson, K. J. Ion dynamics in RFP and spheromak plasmas. Manchester: UMIST, 1993.

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Massachusetts. Executive Office for Administration and Finance. PCA research project: Request for proposals (RFP). Boston, Mass: Commonwealth of Massachusetts, Executive Office for Administration and Finance, 1993.

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Rouben, B. Overview of current RFSP-code capabilities for CANDU core analysis. Mississauga, Ont: AECL, 1996.

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Book chapters on the topic "RFLP"

Leppla, Norman C., Bastiaan M. Drees, Allan T. Showler, John L. Capinera, Jorge E. Peña, Catharine M. Mannion, F. William Howard, et al. "RFLP." In Encyclopedia of Entomology, 3178. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_3379.

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Frank, J. Howard, J. Howard Frank, Michael C. Thomas, Allan A. Yousten, F. William Howard, Robin M. Giblin-davis, John B. Heppner, et al. "PCR-RFLP." In Encyclopedia of Entomology, 2766. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_2812.

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Kochert, Gary. "RFLP technology." In Advances in Cellular and Molecular Biology of Plants, 8–38. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-1104-1_2.

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Brettschneider, Reinhold. "RFLP Analysis." In Molecular Tools for Screening Biodiversity, 85–95. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-009-0019-6_18.

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Jahoor, Ahmed. "RFLP Markers." In Molecular Tools for Screening Biodiversity, 229–36. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-009-0019-6_45.

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Kleinhofs, Andris, and Andrzej Kilian. "RFLP maps of barley." In Advances in Cellular and Molecular Biology of Plants, 163–98. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-1104-1_10.

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Coe, Edward H., and Jack M. Gardiner. "RFLP maps of maize." In Advances in Cellular and Molecular Biology of Plants, 240–45. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-1104-1_13.

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Halward, Tracy, H. Thomas Stalker, and Gary Kochert. "RFLP map of peanut." In Advances in Cellular and Molecular Biology of Plants, 246–60. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-1104-1_14.

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Shoemaker, Randy C. "RFLP map of soybean." In Advances in Cellular and Molecular Biology of Plants, 299–309. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-1104-1_19.

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Stalker, H. Thomas, Tracy Halward, and Gary Kochert. "RFLP map of peanut." In Advances in Cellular and Molecular Biology of Plants, 285–99. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-015-9815-6_16.

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Conference papers on the topic "RFLP"

Moodie, P., I. R. Peake, M. B. Liddell, and A. L. Bloom. "CARRIER DETECTION AND PRENATAL DIAGNOSIS IN HAEMOPHILIA A BY GENE ANALYSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644007.

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Restriction fragment length polymorphism (RFLP) analysis has been used to perform family studies, including prenatal diagnosis, in 21 haemophilia A kindred.Two intragenomic RFLPs were studied in conjunction with one linked RFLP. The intragenomic BgII RFLP,situation 3' to exon 26 was detected with cDNA probe C (Genetics Institute) giving bands of 20kb (17% of X chromosomes) and 5kb (83%), and the intragenomic Bell RFLP, situated 3' to exon 18, was detected with the genomic DNA probe pi 14.12 from Genentech. The frequency of this RFLP in the local population was 23% (1.1 kb allele) and 77% (0.88kb allele). The linked probe DXS15 (DX13) was used to detect a Bglll RFLPwith alleles of 5.8kb (45%) and 2.8kkb (55%). A recombination rate of approximately 5% has been estimated between the factor VIII and DXS15 lociCarrier studies were performed in 15 kindreds. 25 obligate carriers were identified and of these, 20 were potentially informative (heterozygous and phase known) for at least 1 RFLP (9 for Bgll, 9 for Bell and 7 for BgIII). 34 possible carriers were studied, of which 13 were diagnosed as normal (6 by BgII, 6 by Bell and 5 by BgIII). 17 were diagnosed as carriers (2 by Bgll, 12 by Bell and 10 by Bglll) and diagnosis was not possible in a further 4 cases. Of these diagnosed as carriers 3 were non-informative for all RFLPs, and 14informative for at least one RFLP (3 by Bgll, 8 by Bell and 8 with BgIII).Prenatal diagnosis was attempted by analysis of DNA extracted by chorionic villussampling in 6 cases of male fetuses at risk of havinghaemophilia A. 1 fetus was diagnosed as being affected (Bell) and was electively terminated. Three otherfetuses were diagnosed as normal by the BgIII/DXS15 RFLP, but the two intragenomic RFLPs were non-informative. Because of the possibility of a crossover allthree patients opted for mid-trimester fetoscopy andmeasurement of fetal factor VIII at Kings College Hospital, London (Dr Reuben Mibashan), where the diagnoses were confirmed. In the 4th case a normal fetus was diagnosed by the Bgll RFLP analysis, but a spontaneous abortion at 12 weeks prevented confirmation of this result. In the final case of twin male fetuses, none of the RFLPs was informative and both were diagnosed as normal by fetal blood sampling at fetoscopy.

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Lillicrap, D., A. R. Giles, J. J. A. Holden, and B. N. White. "THE RELATIVE EFFICACY OF GENETIC ANALYSIS AND COAGULATION TESTING IN THE DIAGNOSIS OF CARRIERS OF HEMOPHILIA A." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644010.

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This study has assessed the relative benefits of restriction fragment length polymorphism (RFLP) linkage and coagulation testing in the diagnosis of carriers of hemophilia A. 221 samples from 55 families have been studied for intragenic and flanking RFLPs. All samples were tested for the Factor VIII intragenic Bell RFLP and for the flanking marker St 14. 83% of obligate carrier females were heterozygous at oneor both of these two polymorphicsites. However, only38% of these women were heterozygous at the intragenic site and might safely be offered prenatal diagnosis using this marker for the hemophilia mutation. Carrier diagnosis was obtained in 52% of 81 potential carriers tested. Diagnosis wasbased on intragenic RFLP information in only 48% of these cases. Genetic diagnosis was possible in 27 atrisk women from families with no prior history of hemophilia. Four of these women were diagnosed as carriers on the basis of a gross Factor VIII gene deletion and the remaining 23 women were identified as non-carriers by the Bell (11) and Stl4 (12) RFLP data. 39 women remained undiagnosed after gene analysis studies. 23 of these women were female relatives of sporadic hemophiliacs and thus RFLP segregation analysis was inappropriate. A further 9 potential carriers were undiagnosed because of homozygosity in key individuals in their families. In 31 potential carriers we have quantitated Factor VIII:C (one stage assay) and vWf:Ag (Laurell and ELISA) and derived probabilities for carrier status. In 3 women there was conflicting genetic and coagulation data. Meanwhile, in 12 undiagnosed women from sporadic families, carrier diagnostic probabilities of > 0.9 were obtained. These studies indicate that optimal carrier detection for hemophilia A requires more intragenic and closely linked RFLPs and the continuance of coagulation testing to assist women from sporadic families.

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Bernardi, F., G. Marchetti, F. Vannini, L. Felloni, F. Panicucci, and F. Conconi. "SPORADISM INVESTIGATION AND CARRIER DETECTION IN HAEMOPHILIA A BY RFLP ANALYSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644011.

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Restriction fragment lenght polymorphisms (RFLPs)analysis has been employed for carrier detection andfor sporadism study in Haemophilia A. Three RFLPs, one intragenic in FC8 (647/BcII) and two with close linkage to Haemophilia A at DXS52 (Stl4/Taql) and DXS15 (DX13/BgIII), were used.In 20 families 29 carrier status determinations havebeen performed.In order to investigate sporadicity and to estimate the sex ratio of mutation rates directely, 17 families with isolated cases of haemophilia A were studied.In eight out of the 17 families the RFLPsanalysis excluded the carrier status of the maternalgrandmothers.Since by hemostatic studies the eight mothers of the propositi were shown to be haemophilia carriers, the origin of the newly mutated genes was inferred from the RFLP patterns: six haemophilic genes derive from the normal maternal grandfathers and two from the maternal grandmothers.Possible recombinations between FVIII locus and the extragenic RFLPs loci have to be considered; however the intragenic Bell RFLP is informative in five out of the eight families and the DXl3 and Stl4 patterns are concordant.The data indicate a higher mutation rate in males than in females gametes as previously suggested, althought not unanimously, by segregation analysis and coagulation studies. The RFLP analysis in a large number of families with isolated cases of haemophilia isnecessary to define the precise ratio of sex mutation rate for this disease.Work supported by P.F. Ing. Gen. Basi Mol. Mai. Ered. Contratto CNR N 8400877.

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Liddell, M. B., D. S. Anson, D. P. Lillicrap, and I. R. Peake. "SEARCH FOR AND USE OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLPs) IN AND AROUND THE HUMAN FACTOR IX GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644078.

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5 previously described RFLPs within the factor IX gene have been used for family studies (carrier detection) in 10 haemophilia B kindred. In all DNA from 91 individuals, including 25 obligate or possible carriers, was analysed by digestion with TaqI and XmnI and probing with the intragenomic probe VIII (all probes were provided by Professor G. G. Brownlee, Oxford). When noninformative, additional RFLPs (DdeI;probe XIII and MspI;probe II) were used. Of 12 possible carriers, 11 were diagnosed (6 as carriers, 5 normal). Of the confirmed carriers (6 diagnosed, 13 obligate) 15 were informative (heterozygous and phase known), and the overall incidence of heterozygosity was 72%. The recently reported BamHI RFLP was not found to be useful ( <1.0% frequency).Further RFLPs in and flanking the factor IX gene were sought by two procedures. Firstly cosmid pCHIXα, containing a 40kb insert including the 3' end of the factor IX gene and stretching some 35kb 3' to the gene was used as a large probe, with repetitive sequences being blocked by preannealing the probe with an excess of sonicated, denatured human DNA (Litt and White, PNAS 82, 6206). Results with 25 restriction enzymes (covering an estimated 1038 nucleotides) and DNA from 7 unrelated females were obtained, but only one low frequency PvuII RFLP (frequency about 1%) was identified. Similar experiments with further cosmid probes 3' to the gene are underway. The second technique was developed to analyse small DNA fragments (<1.0kb) generated by frequently cutting restriction enzymes. These fragments were separated on 3.5% polyacrylamide/0.5% agarose composite gels and then electroblotted onto hybond-N. Fragments of 150bp were readily visualised by this procedure. 3 frequently cutting enzymes have been used (Hinfl, Rsal and Mbol), and the blots probed with a factor IX c-DNA probe, or a unique sequence subclone of cosmid pCHIXα. To date no RFLPs have been identified. This search for further useful RFLP has illustrated the paucity of detectable sequence variation within this region of the X-chromosome.

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Iris, Grasler, Wiechel Dominik, and Oleff Christian. "Extended RFLP for complex technical systems." In 2022 IEEE International Symposium on Systems Engineering (ISSE). IEEE, 2022. http://dx.doi.org/10.1109/isse54508.2022.10005424.

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Marchetti, G., S. Guerra, G. Ballerini, P. Patracchini, S. Volinia, and F. Bernardi. "A FREQUENT TAQI RFLP AND A GENE LESION OR RARE TAQI RFLP IN THE VON WILLEBRAND FACTOR GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642834.

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A cDNA for vonWi11ebrand factor (vWf) has been used to investigate lesions and RFLPs in the vWf gene.In hybridizations with the 3'cDNA portion (a 2 Kb SacI fragment) a frequent polymorphism has been found with TaqI restriction enzyme. The alleles are 3.3 Kb and 2.6 Kb ; the frequencies of 0.51 and 0.49 respectively enable to investigate an appreciable portion of vW disease (vWd) families.In a patient with type III vWd an abnormal TaqI pattern has been observed. A 4.5 Kb band is absent and an additional band of 2.3 Kb is present.This pattern has been inherited from the consanguineous heterozygous parents and has been traced in several members of this large family. The presence of the abnormal gene pattern is related to total or partial vWf deficiency in the family and has not been found in several normal subjects. The BamHI and BglII restriction patterns are normal and suggest a small mutation originating a new TaqI site.These findings are compatible with a gene lesion or a rare RFLP.Work supported by Ricerca Sanitaria Finalizzata Regione Emilia Romagna.

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Chuang, Li-Yeh, Yu-Da Lin, Hsueh-Wei Chang, and Cheng-Hong Yang. "An Improved Natural PCR-RFLP Primer Design Method." In Bioengineering (BIBE). IEEE, 2011. http://dx.doi.org/10.1109/bibe.2011.21.

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Horvath, Laszlo, and Imre J. Rudas. "Content driven generation of RFLP structured Engineering System representation." In 2016 World Automation Congress (WAC). IEEE, 2016. http://dx.doi.org/10.1109/wac.2016.7582958.

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Maramis, Christos, and Anastasios Delopoulos. "Efficient Quantitative Information Extraction from PCR-RFLP Gel Electrophoresis Images." In 2010 20th International Conference on Pattern Recognition (ICPR). IEEE, 2010. http://dx.doi.org/10.1109/icpr.2010.627.

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Llerena, S. Espezua, and C. D. Maciel. "Exploratory Visualization of RFLP-PCR Genomic Data Using Multidimensional Scaling." In 2008 XXI Brazilian Symposium on Computer Graphics and Image Processing (SIBGRAPI). IEEE, 2008. http://dx.doi.org/10.1109/sibgrapi.2008.42.

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Reports on the topic "RFLP"

Tanksley, Steven, and Dani Zamir. Tagging Plant Genes with Tightly-Linked RFLP Markers. United States Department of Agriculture, January 1992. http://dx.doi.org/10.32747/1992.7603794.bard.

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Kistler, Harold Corby, Talma Katan, and Dani Zamir. Molecular Karyotypes of Pathogeic Strains of Fusarium oxysporum. United States Department of Agriculture, June 1995. http://dx.doi.org/10.32747/1995.7604927.bard.

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Genetic diversity of pathogenic strains of the fungus Fusarium oxysporum was determied by analysis of electrophoretic karyotype, as well as by DNA variation detected by Restriction Fragment Length Polymorphisms (RFLPs) and Random Amplified Polymorphic DNAs (RAPDs). The electrophoretic karyotypes for 130 isolates of the fungus pathogenic to tomato, melon, and banana were analyzed. Electrophoretic karyotype variation, reflected in differences in apparent chromosome number and genome size, was observed even among isolates from the same host and sub specific category. Sub specific categories studied were forma specialis, vegetative compatibility group (VCG) and race. Chromosome number and genome size variation was less for isolates within the same VCG than for the collection of isolates as a whole. RFLP and RAPD analysis were performed on 62 isolates of F. oxysporum from tomato and melon. Polygenetic trees were constructed from genetic diversity data. The results support the hypothesis that isolates belonging to the same VCG originate from a single ancestor compared to other isolates. The results do not support the hypothesis that all isolates belonging to the same forma specialis originate from a common ancestor. These conclusions have profound implication for breeding resistance to diseases caused by particular formae speciales of F. oxysporum.

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Michel Jr., Frederick C., Harry A. J. Hoitink, Yitzhak Hadar, and Dror Minz. Microbial Communities Active in Soil-Induced Systemic Plant Disease Resistance. United States Department of Agriculture, January 2005. http://dx.doi.org/10.32747/2005.7586476.bard.

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Induced Systemic Resistance (ISR) is a highly variable property that can be induced by compost amendment of potting media and soils. For example, previous studies showed that only 1 of 79 potting mixes prepared with different batches of mature composts produced from several different types of solid wastes were able to suppress the severity of bacterial leaf spot of radish caused by Xanthomonas campestris pv. armoraciae compared with disease on plants produced in a nonamended sphagnum peat mix. In this project, microbial consortia in the rhizosphere of plants grown in ISR-active compost-amended substrates were characterized. The plants used included primarily cucumber but also tomato and radish. Rhizosphere microbial consortia were characterized using multiple molecular tools including DGGE (Israel) and T -RFLP (Ohio) in both ISR-active field plots and potting media. Universal as well as population-specific bacterial and fungal PCR primers were utilized. T -RFLP analyses using universal bacterial primers showed few significant differences in overall bacterial community composition in ISR-active and inactive substrates (Ohio). In addition, the community members which were significantly different varied when different ISR-activecomposts were used (Ohio). To better characterize the shifts in microbial community structure during the development of ISR, population specific molecular tools were developed (Israel, Ohio).-PCR primers were designed to detect and quantify bacterial groups including Pyrenomycetes, Bacillus, Pan toea, Pseudomonas, Xanthomonas and Streptomyces as well as Trichoderma and Fusarium; two groups of fungi that harbor isolates which are ISR active (Isreal and Ohio). Bacterial consortia associated with cucumber plants grown in compost-amended potting mixtures were shown to be dominated by the phylogenetic taxon Bacteroidetes, including members of the genus Chryseobacterium, which in some cases have been shown to be involved in biocontrol (Israel). Nested-PCR-DGGE analyses coupled with long l6S rDNA sequencing, demonstrated that the Chryseobacteriumspp. detected on seed and the root in compost-amended treatments were derived from the compost itself. The most effective ISR inducing rhizobacterial strains were identified as Bacillus sp. based on partial sequencing of l6S rDNA. However, these strains were significantly less effective in reducing the severity of disease than Trichoderma hamatum382 (T382). A procedure was developed for inoculation of a compost-amended substrate with T -382 which consistently induced ISR in cucumber against Phytophthora blight caused by Phytophthora capsiciand in radish against bacterial spot (Ohio). Inoculation of compost-amended potting mixes with biocontrol agents such as T -382 and other microbes that induce systemic resistance in plants significantly increased the frequency of systemic disease control obtained with natural compost amendments.

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Perl-Treves, Rafael, M. Kyle, and Esra Galun. Development and Application of a Molecular Genetic Map for Melon (Cucumis melo). United States Department of Agriculture, October 1993. http://dx.doi.org/10.32747/1993.7568094.bard.

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This project has generated a systematic survey of DNA polymorphism in Cucumis melo. An RFLP and RAPD survey of the major cultivar groups and botanical varieties of this species has been conducted, with the purpose of assessing the degree of molecular variation and phylogenetic relationships within the melon germplasm and, at the same time, develop sets of markets suitable for mapping the melon genome. Additional activities regarding variation in the melon germplasm in fruit traits and regeneration ability have been initiated as well. The necessary populations required for the development of a molecular map of the C. melo genome have been prepared. An F2 that segregated for 4 viral resistances, powdery mildew resitance and sex type has been derived from a PI 414723 x Topmark cross, and a RILs population has been prepared from it. We have confirmed the resistances in the population and have analyzed the genetic relationships between these resistances. Progress toward the construction of a molecular map of C. melo and the development of markers linked to those traits is described. We have so far screened the first few tens of markers in the F2 population, and many additional ones were screened in DNA bulks prepared from such population.

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Sink, Ken, Shamay Izhar, and Abraham Nachmias. Asymmetric Somatic Hybridization: Developing a Gene Transfer System for Solanaceous Vegetable Crops. United States Department of Agriculture, February 1996. http://dx.doi.org/10.32747/1996.7613010.bard.

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Highly asymmetric somatic hybrid plants were obtained by PEG/DMSO fusion of gamma irradiated (100, 250, 7500 and 1000 Gy) protoplasts of a (KmR-) interspecific hybrid Lycopersicon esculentum x L. pennellii (EP) with protoplasts of eggplant (E). Somatic hybrid calli were selected based on kanamycin resistance and verified by PCR of the NptII gene, RAPD's and Southern's using potato rDNA pTHG2 probes. Flow cytometry indicated all hybrid calli that did not regenerate shoots were 5-9n. Three asymmetric plants regenerated only from callus close to 4n and such calli oly occurred when EP received 100 Gy. The asymmetric plants had eggplant morphology and regenerated from one hybrid callus with 6.29 average size tomato chromosomes. Limited amounts of EP DNA were found in the three somatic hybrid plants H18-1 to -3 by dot-blot hybridization with probe pTHG2, to be equivalent to 6.23, 5.41, and 5.95 % EP, respectively. RFLP analysis of Lycopersicon esculentum and L. pennellii specific chromosomes revealed that only fragments of 8 to 10 out of the 24 EP chromosomes are present in the asymmetric plants. Transgenic plants 2-3, 2-4 and 10-3 were found resistant to verticillium; suggesting successful transfer of the Ve complex from S. torvum to eggplant.

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Dubcovsky, Jorge, Tzion Fahima, and Ann Blechl. Positional cloning of a gene responsible for high grain protein content in tetraploid wheat. United States Department of Agriculture, September 2003. http://dx.doi.org/10.32747/2003.7695875.bard.

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High Grain Protein Content (GPC) is a desirable trait in breadmaking and pasta wheat varieties because of its positive effects on quality and nutritional value. However, selection for GPC is limited by our poor understanding of the genes involved in the accumulation of protein in the grain. The long-term goal of this project is to provide a better understanding of the genes controlling GPC in wheat. The specific objectives of this project were: a) to develop a high-density genetic map of the GPC gene in tetraploid wheat, b) to construct a T. turgidum Bacterial Artificial Chromosome (BAC) library, c) to construct a physical map of the GPC gene and identify a candidate for the GPC gene. A gene with a large effect on GPC was detected in Triticum turgidum var. dicoccoides and was previously mapped in the short arm of chromosome 6B. To define better the position of the Gpc-B1 locus we developed homozygous recombinant lines with recombination events within the QTL region. Except for the 30-cM region of the QTL these RSLs were isogenic for the rest of the genome minimizing the genetic variability. To minimize the environmental variability the RSLs were characterized using 10 replications in field experiments organized in a Randomized Complete Block Design, which were repeated three times. Using this strategy, we were able to map this QTL as a single Mendelian locus (Gpc-B1) on a 2.6-cM region flanked by RFLP markers Xcdo365 and Xucw67. All three experiments showed that the lines carrying the DIC allele had an average absolute increase in GPC of 14 g/kg. Using the RFLP flanking markers, we established the microcolinearity between a 2.l-cM region including the Gpc-B1 gene in wheat chromosome 6BS and a 350-kb region on rice chromosome 2. Rice genes from this region were used to screen the Triticeae EST collection, and these ESTs were used to saturate the Gpc-B1 region with molecular markers. With these new markers we were able to map the Gpc-B1 locus within a 0.3-cM region flanked by PCR markers Xucw83 and Xucw71. These flanking markers defined a 36-kb colinear region with rice, including one gene that is a potential candidate for the Gpc-B1 gene. To develop a physical map of the Gpc-B1 region in wheat we first constructed a BAC library of tetraploid wheat, from RSL#65 including the high Gpc-B1 allele. We generated half- million clones with an average size of l3l-kb (5.1 X genome equivalents for each of the two genomes). This coverage provides a 99.4% probability of recovering any gene from durum wheat. We used the Gpc-BI flanking markers to screen this BAC library and then completed the physical map by chromosome walking. The physical map included two overlapping BACs covering a region of approximately 250-kb, including two flanking markers and the Gpc-B1 gene. Efforts are underway to sequence these two BACs to determine if additional wheat genes are present in this region. Weare also developing new RSLs to further dissect this region. We developed PCR markers for flanking loci Xucw79andXucw71 to facilitate the introgression of this gene in commercial varieties by marker assisted selection (httQ://maswheat.ucdavis.edu/ orotocols/HGPC/index.hlm). Using these markers we introgressed the Gpc-B1 gene in numerous pasta and common wheat breeding lines.

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Reisch, Bruce, Pinhas Spiegel-Roy, Norman Weeden, Gozal Ben-Hayyim, and Jacques Beckmann. Genetic Analysis in vitis Using Molecular Markers. United States Department of Agriculture, April 1995. http://dx.doi.org/10.32747/1995.7613014.bard.

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Genetic analysis and mapping in grapes has been difficult because of the long generation period and paucity of genetic markers. In the present work, chromosome linkage maps were developed with RAPD, RFLP and isozyme loci in interspecific hybrid cultivars, and RAPD markers were produced in a V. vinifera population. In three cultivars, there were 19 linkage groups as expected for a species with 38 somatic chromosomes. These maps were used to locate chromosome regions with linkages to important genes, including those influencing powdery mildew and botrytis bunch rot resistance; flower sex; and berry shape. In V. vinifera, the occurrence of specific markers was correlated with seedlessness, muscat flavor and fruit color. Polymorphic RAPD bands included single copy as well as repetitive DNA. Mapping procedures were improved by optimizing PCR parameters with grape DNA; by the development of an efficient DNA extraction protocol; and with the use of long (17- to 24-mer) primers which amplify more polymorphic loci per primer. DNA fingerprint analysis with RAPD markers indicated that vinifera cultivars could be separated readily with RAPD profiles. Pinot gris, thought to be a sort of Pinot noir, differed by 12 bands from Pinot noir. This suggests that while Pinot gris may be related to Pinot noir, it is not likely to be a clone. The techniques developed in this project are now being further refined to use marker-assisted selection in breeding programs for the early selection of elite seedlings. Furthermore, the stage has been set for future attempts to clone genes from grapes based upon map locations.

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Katzir, Nurit, Rafael Perl-Treves, and Jack E. Staub. Map Merging and Homology Studies in Cucumis Species. United States Department of Agriculture, September 2000. http://dx.doi.org/10.32747/2000.7575276.bard.

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List of original objectives (1) Construct a saturated map of melon, using RFLP, SSR, RAPD and Inter-SSR genetic markers. (2) Study the homology between the genomes of cucumber and melon. (3) Add to the Cucumis map, biologically important genes that had been cloned in other plant systems. Background Cucumber and melon are important vegetable crops in Israel and the US. Genome analysis of these crops has lagged behind the major plant crops, but in the last few years genetic maps with molecular markers have been developed. The groups that participated in this program were all involved in initial mapping of cucurbit crops. This grant was meant to contribute to this trend and promote some of the more advanced applications of genome analysis, i.e., map saturation and comparative mapping between cucurbit species. Major achievements The main achievements of the research were (a) the construction of melon maps that include important horticultural traits and Resistance Gene Homologues, (b) the development of approximately 200 SSR markers of melon and cucumber, (c) the preliminary map merging of melon maps and of comparative mapping between melon and cucumber. Implications As a result of this program, we have a good estimate of the applicability of different types or markers developed in one cucurbit species to genetic mapping in other species. Since the linkage groups of melon and cucumber can now be related to each other, future identification of important genes in the two crops will be facilitated. Moreover, the further saturation of the maps with additional markers will now allow us to target several disease resistance loci, horticultural traits for marker-assisted selection, fine mapping and positional cloning.

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Aldrich, Susan. Product Search Solution RFP Template. Boston, MA: Patricia Seybold Group, March 2004. http://dx.doi.org/10.1571/ii3-18-04cc.

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Lees, Matthew. Online Community Platform RFP Template. Boston, MA: Patricia Seybold Group, July 2009. http://dx.doi.org/10.1571/rfp07-16-09cc.

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