Dissertations / Theses on the topic 'Rfc1'
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Breitenbücher, Frank. "Allgemeine und funktionelle Untersuchungen zur grossen Untereinheit des humanen Replikationsfaktors C (RFC1), sowie initiale Untersuchungen zur Regulation der Expression von RFC1 durch das BCR-ABLp210-Onkogen." [S.l.] : [s.n.], 2005. http://archiv.ub.uni-marburg.de/diss/z2005/0114/.
Hinken, Matthias. "Gewebeverteilung und Lokalisation des Transportproteins für reduzierte Folate (RFC1) der Ratte." Doctoral thesis, Universitätsbibliothek Leipzig, 2007. http://nbn-resolving.de/urn:nbn:de:bsz:15-20071107-143055-8.
Chazelas, Pauline. "Syndrome de CANVAS – Analyse moléculaire des répétitions pentanucléotidiques du gène RFC1 et étude de leurs conséquences physiopathologiques." Electronic Thesis or Diss., Limoges, 2024. http://www.theses.fr/2024LIMO0006.
The CANVAS syndrome defines a group of clinical entities - cerebellar ataxia, sensory neuronopathy and vestibular areflexia - that together or separately are responsible for a variety of symptoms, the most common of which is a disturbance of balance. Interestingly, it has now been shown that the majority of patients present with a chronic refractory cough decades before the onset of neurological disorders. In 2019, the genetic cause of this syndrome was described. It involves the RFC1 gene, in which a biallelic expansion of AAGGG pentanucleotide repeats in intron 2 of the gene has been implicated in clinically affected patients. This large AAGGG motif replaces the AAAAG conformation, which is classically repeated 11 times in the general population. The pathophysiological cause of the disease is still unknown. Several studies have shown variability in the repeat zone, both in terms of motif conformation and number of repeats. The implications for pathology are not always clear.In this study, we focused on three aspects of this syndrome: (a) description of the variability of the repeated zone in a cohort of controls and in a cohort of patients referred for neuropathy, (b) histological study of peripheral nerve damage, particularly that of small nerve fibers, which has not been previously described, (c) study of the prevalence of biallelic expansion of the pentanucleotide AAGGG in a cohort of refractory chronic cough patients.Our results revealed novel complex repeats in both controls and patients with neuropathy. The involvement of small nerve fibers in CANVAS was objectified for the first time. Our cohort study of 68 patients with refractory chronic cough identified 16% of patients with a pathogenic AAGGG biallelic expansion, leading us to suggest that the cough of CANVAS patients is neurogenic.Taken together, the results of this thesis represent a breakthrough in the molecular and pathophysiological knowledge of CANVAS syndrome. Further work is required to understand the underlying pathophysiological mechanisms with a view to developing personalized therapies
Halwachs, Sandra. "Regulation des Reduced Folate Carrier (RFC1) in HPCT-1E3-Ratten-Hepatocytoma-Zellen durch Cytochrom P450-Induktoren vom Phenobarbital-Typ." Doctoral thesis, Universitätsbibliothek Leipzig, 2006. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-34397.
Lopes, Tairine Zara. "Avaliação de polimorfismos nos genes MTHFR, MTR, RFC1 e CßS envolvidos no metabolismo do folato em pacientes com câncer de tireoide." Faculdade de Medicina de São José do Rio Preto, 2015. http://hdl.handle.net/tede/272.
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Introduction: Thyroid cancer is the most common malignancy of the endocrine system and has been presenting continuous increase in the last years. Studies suggest that folate deficiency in the body decrease DNA repair, resulting in malignant cells changes that alter expression of genes, and may induce several kinds of cancer development. Polymorphisms in genes involved in folate pathway have been investigated as risk factors for susceptibility to cancer, among them MTHFR, MTR, RFC1 and CßS. Objectives: To investigate association of polymorphisms in the MTHFR (C677T), MTR (A2756G), RFC1 (A80G) and CßS (844ins68) genes in risk thyroid cancer in a case-control study; to evaluate the association of polymorphisms with gender, age, alcohol and tobacco consumption, body-mass index in thyroid cancer development; and to evaluated the association between polymorphisms and clinical-histopathological parameters. Methods: This study included 462 individuals (151 patients with thyroid cancer and 311 controls). The peripheral blood was collected and genomic DNA was extracted. The MTHFR (C677T), MTR (A2756G) and RFC1 (A80G) were evaluated by PCR-RFLP and CßS (844ins68) by conventional PCR without enzymatic digestion. For statistical analysis chi-square and multiple logistic regression were used. Results: The results showed that MTHFR C677T (OR=2.87, 95% CI=1.50-5.48, p< 0.01, codominant model), (OR=1.76, 95% CI=1.18-2.64, p< 0.01, dominant model), (OR=2.37, 95% CI=1.28-4.39, p< 0.01, recessive model) and RFC1 A80G (OR: 1.55; 95% CI: 1.02-2.38; p=0.04, recessive model) were associated with thyroid cancer. The alcohol (OR=1.56, 95% CI=1.36-1.89, p< 0.01) and tobacco consumption (OR=1.97, 95% CI=1.28-3.04, p< 0.01) were statistically significant, being associated with increased risk. The MTR A2756G is associated with tumor extension (OR=2.69, 95% CI=1.27-5.71, p< 0.01) and aggressiveness (OR= 4.51, 95% CI=1.67-12.1, p< 0.01). Conclusions: The MTHFR (C677T) and RFC1 (A80G) polymorphisms were involved in risk for thyroid cancer. Additionally, alcohol and tobacco consumption increase risk for disease development.
Introdução: O câncer de tireoide é a neoplasia maligna mais comum do sistema endócrino e vem apresentando contínuo aumento nos últimos anos. Estudos sugerem que a deficiência de folato no organismo diminui a reparação do DNA, resultando em alterações celulares malignas que modulam a expressão gênica, podendo levar ao desenvolvimento de vários tipos de câncer. Polimorfismos em genes envolvidos na via do folato têm sido investigados como fatores de risco para suscetibilidade ao câncer, entre eles, polimorfismos nos genes MTHFR, MTR, RFC1 e CßS. Objetivos: Investigar a associação dos polimorfismos nos genes MTHFR (C677T), MTR (A2756G), RFC1 (A80G) e CßS (844ins68) no risco de câncer de tireoide em um estudo caso-controle; Avaliar a associação dos polimorfismos com o gênero, idade, consumo de álcool e tabaco, índice de massa corpórea (IMC) no desenvolvimento do câncer de tireoide; Avaliar a associação entre os polimorfismos e os parâmetros clínico-histopatológicos do câncer de tireoide. Casuística e Método: Este estudo incluiu 462 indivíduos (151 pacientes com câncer de tireoide e 311 indivíduos controles). Foi coletado sangue periférico e extraído o DNA genômico. Os polimorfismos MTHFR (C677T), MTR (A2756G) e RFC1 (A80G) foram avaliados por meio da PCR-RFLP e o polimorfismo CßS (844ins68) foi analisado por PCR convencional sem corte enzimático. Para análise estatística utilizou-se o teste do qui-quadrado e regressão logística múltipla. Resultados: Os resultados mostraram que os polimorfismos MTHFR C677T (OR=2.87, 95% IC=1.50-5.48, p< 0.01, modelo codominante), (OR=1.76, 95% IC=1.18-2.64, p< 0.01, modelo dominante), (OR=2.37, 95% IC=1.28-4.39, p< 0.01, modelo recessivo) e RFC1 A80G (OR: 1.55; 95% IC: 1.02-2.38; p=0.04, modelo recessivo) estão associados ao câncer de tireoide. O consumo de álcool (OR=1.56, 95% IC=1.36-1.89, p< 0.01) e tabaco (OR=1.97, 95% IC=1.28-3.04, p< 0.01) foram estatisticamente significantes, sendo associados ao aumento do risco. O polimorfismo MTR A2756G está associado à extensão do tumor (OR=2.69, 95% IC=1.27-5.71, p< 0.01) e à agressividade (OR= 4.51, 95% IC=1.67-12.1, p< 0.01). Conclusões: Os polimorfismos MTHFR (C677T) e RFC1 (A80G) estão envolvidos no risco de câncer de tireoide. Adicionalmente, o consumo de álcool e tabaco aumenta o risco de desenvolvimento da doença.
Tomida, Junya. "DNA damage induced ubiquitylation of RFC2 subunit of RFC complex." Kyoto University, 2008. http://hdl.handle.net/2433/135870.
Ng, Chin Chin. "Performance analysis of the mobile IP protocol (RFC 3344 and related RFCS)." Thesis, Monterey, Calif. : Naval Postgraduate School, 2006. http://bosun.nps.edu/uhtbin/hyperion.exe/06Dec%5FNg%5FChin.pdf.
Thesis Advisor(s): George W. Dinolt, J. D. Fulp. "December 2006." Includes bibliographical references (p. 121-125). Also available in print.
Hakim, Chantal DDS MSD, Marie M. MD PhD DrSc Tolarová, and Miroslav MD PhD Tolar. "Association of Type and Severity of Nonsyndromic Orofacial Clefts with Combined Genotypes of RFC1 80 GA, MTHFR 677 CT, IRF6 (rs642961) and IRF6 (rs2235371) Gene Polymorphisms in an Indian Population." Scholarly Commons, 2020. https://scholarlycommons.pacific.edu/dugoni_etd/2.
Giusti, Kelma Cordeiro da Silva. "Associação entre polimorfismos em genes relacionados ao metabolismo de folato (RFC1, GCP2, MTHFR e MTHFD1) e alterações nas concentrações de folato, cobalamina e homocisteína em mulheres com história de abortos espontâneos recorrentes." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-08032013-115754/.
The recurrent spontaneous abortion (RSA) is characterized by the occurrence of three or more consecutive miscarriages and affects 2-4% of women of childbearing age. The etiology is associated with several risk factors such as uterine abnormalities, chromosomal aberrations, autoimmunity, thrombophilia, increased concentration of homocysteine (tHcy). About 40% of cases remains unknown cause. The units of carbon metabolism plays an essential role in the availability of the cell folate, is essential for the placental and fetal development. A deficiency of the vitamins that regulate this metabolism, like folic acid, and polymorphisms in genes encoding enzymes related to folate metabolism (MTHFR, RFC1, and GCP2 MTHFD1) may lead to decreased concentrations of this vitamin and increased concentrations of tHcy. Objective was to evaluate the association between polymorphisms in genes related to folate metabolism (RFC1, GCP2, MTHFD1 and MTHFR) and the risk of having AER, and to evaluate the association between these polymorphisms and changes in concetranções folate, cobalamin, and homocysteine. Three groups were divided: AER primary: 117 women with RSA and no viable fetus, AER secondary: 139 women with RSA and at least one viable fetus and Control: 264 women with no history of miscarriage. None of the women was pregnant at time of blood collection. Blood samples were taken for biochemical (folate, Cbl, tHcy, etc.), immunological and genomic DNA extraction. The genotyping were carried out by PCR-RFLP or real time PCR. Serum concentrations of folate and Cbl were higher in groups 1 and 2 (p <0.05). The distribution of genotypes of MTHFR c.677C> T, MTHFR c.1298A> C, MTHFD1 c.1958G> A, RFC1 c.80G>GCP2 A and c.1561C> T was similar among the three groups. The increased concentrations of serum folate (OR: 1.05, 95% CI: 1.03 - 1.07, p <0.001), Cbl (OR: 1.00, 95% CI: 1.00 to 1.00, p = 0.016), tHcy (OR: 1.03, 95% CI: 0.97 to 1.11, p = 0.033) and T4 (OR: 1.02, 95% CI: 1.00 to 1.03, p = 0.006) and the presence of ANA (1:160) (OR: 2.90, 95% CI: 1.25 - 6.75, p = 0.013) were considered risk factors primary for abortion. For secondary abortion, were considered risk factors increased the concentrations of serum folate (OR: 1.04, 95% CI: 1.02 - 1.05, p <0.001), cobalamin (OR: 1.00, 95 % CI: 1.00 to 1.00, p = 0.019) and tHcy (OR: 1.05, 95% CI: 1.00 to 1.09, p = 0.039), higher age (OR: 1.02, 95% CI: 0.98 to 1.06, p = 0.031), cigarette smoking (OR: 2.54, 95% CI: 1.41 to 4.60, p = 0.002) and had a higher BMI (OR : 1,42,95% CI: 1.07 to 1.88, p = 0.015). The studied polymorphisms were not associated with increased risk of having RSA when analyzed separately, and were not associated with changes in serum folate, Cbl and tHcy, with the exception of the MTHFR 677TT genotype, whose patients had a higher concentration of total tHcy compared with those with 677CC and 677CT genotypes in the three groups. The variable concentrations of folate, Cbl, tHcy, and T4, presence of ANA and have been associated with increased risk for miscarriage primary. The variables age, BMI, smoking, concentrations of folate, Cbl and tHcy were associated with increased risk of secondary miscarriage.
Alata, Jimenez Nagif. "La deficiencia en los transportadores de folato (FOLR1 y RFC1) causa cambios epigenéticos que afectan el desarrollo neural y de las células de la cresta neural en embriones de pollo Gallus gallus." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2019. https://hdl.handle.net/20.500.12672/10342.
Tesis
Kim, Jiyoung. "Functional analysis of RFC and RFC-like complexes in fission yeast." Thesis, University of Edinburgh, 2005. http://hdl.handle.net/1842/12375.
Silva, Alan Castro. "Implementação inicial da RFC 6897." Universidade Federal de São Carlos, 2016. https://repositorio.ufscar.br/handle/ufscar/8806.
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The Multipath TCP (MPTCP) protocol allows applications to better explore the network resources available to multi-connected devices such as mobile phones or multi-homed systems. Here, some advantages are envisioned: bandwidth aggregation, the ability to maintain the connection, if one of the network path fails and the use of multiple paths. To extend these capabilities to the application, RFC 6897 defines an API to better control each of MPTCP’s subflows, so that these can be added or removed as needed. This work presents an initial API implementation as defined in RFC 6897. We implemented some functions described in the document, such as protocol on/o, check existent subflows and add new subflows. To test the API and validate our implementation we built an HTTP application that detects elephant flows and uses the API for open new subflows using the original TCP connection. Some tests were performed in a network using a cubic topology and showed that the API utilization decreased the Flow Completion time of TCP connections.
O protocolo Multipath TCP (MPTCP) permite que as aplicações possam explorar melhor os recursos de rede disponíveis para dispositivos multiconectados como os telefones móveis ou sistemas multi-homed. Aqui, algumas vantagens são previstas: agregação de banda, a habilidade de manter a conexão estabelecida se houver falha em um dos caminhos de rede e a utilização de múltiplos caminhos. Para estender essas capacidades para a aplicação, a RFC 6897 define uma API que permite um melhor controle de cada subfluxo MPTCP, de modo que esses possam ser adicionados ou removidos conforme necessário. Este trabalho apresenta uma implementação inicial da API descrita na RFC 6897 para o protocolo MPTCP. Sendo assim, implementamos algumas das funções de manipulação do protocolo MPTCP descritas no documento, quais sejam: ligar e desligar o protocolo, verificar subfluxos existentes e adicionar novos subfluxos. Para testar a API e validar a nossa implementação, nós desenvolvemos uma aplicação HTTP que detecta fluxos elefantes e utiliza a API para abrir novos subfluxos a partir da conexão TCP original. Testes de desempenho foram realizados em uma topologia cúbica e mostraram que a utilização da API pela aplicação diminuiu o Flow Completion Time das conexões TCP.
Mankelow, Rowena Chemistry Faculty of Science UNSW. "Creating mesophorous materials by liquid crystal templating of readily available materials." Awarded by:University of New South Wales, 2003. http://handle.unsw.edu.au/1959.4/38346.
Zhao, Min Chemistry Faculty of Science UNSW. "A fill and flow channel enzyme biosensor." Awarded by:University of New South Wales, 2004. http://handle.unsw.edu.au/1959.4/38333.
Perez-Bercoff, Danielle. "L'activation des macrophages via les RFcγ inhibe la réplication du VIH-1." Paris 7, 2002. http://www.theses.fr/2002PA077144.
Siberil, Sophie. "Etude des interactions moléculaires entre RFcγ et IgG : implications fonctionnelles et thérapeutiques." Paris 6, 2005. http://www.theses.fr/2005PA066549.
Bruhns, Pierre. "Bases moleculaires des proprietes inhibitrices des rfc ii b." Paris 6, 2001. http://www.theses.fr/2001PA066039.
Bartoš, Patrik. "Testování přenosových parametrů internetového připojení." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2021. http://www.nusl.cz/ntk/nusl-442361.
Watson, Joni. "Identification of Transcription Factors GZF3, RFX1, Orf19.3928 as Being Implicated in Candida-Bacterial Interactions." Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/honors/261.
Schrader, Stephanie. "Molekularbiologische und zellbiologische Charakterisierung alternativer Splicing- Varianten des Methotrexat- Carriers (RFC)." Doctoral thesis, Universitätsbibliothek Leipzig, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:15-20090429-113312-2.
Bossard, Florian. "La mucoviscidose : correction de la mutation [delta]F508 par sur-expression de NHE-RF1 : modifications d'expression de NHE-RF1 et des récepteurs [béta]-adrénergiques dans les poumons humains." Nantes, 2007. http://archive.bu.univ-nantes.fr/pollux/show.action?id=6c13703c-0312-4f63-9879-11bdd9fd3d9a.
The present work encompasses two projects. Firstly, we studied the impact of a protein partner of CFTR, NHE-RF1, on the most frequent mutant in cystic fibrosis (CF): deltaF508. NHE-RF1 has been described up to now for its involvement in apical trafficking and membrane stability of wild-type CFTR. Our study showed that NHE-RF1 overexpression rescued the trafficking defect of deltaF508 and rehabilitated a partial channel chloride activity. Secondly, we focused on the expression of the beta-adrenergic receptors (beta-AR) and NHE-RF1 in control and CF human lungs. Indeed, the beta1 and beta2-AR are largely expressed in the lungs where they control various biological functions. But the beta-AR stimulation leads to a faded response in CF. In addition, our team has previously shown that the third beta-AR subtype, beta3-AR, can activate CFTR. But no study has shown the presence of the beta3-AR in human lungs. These data encouraged us to analyze the three beta-AR and NHE-RF1 expressions in human control and CF bronchi samples. We thus showed that: 1) beta3-AR were expressed in human lungs and 2) a remodeling was observed in CF (beta1 and beta3-AR overexpression, beta2-AR and NHE-RF1 under-expression). The physiological relevance of this remodeling remains to be defined. However, NHE-RF1 under-expression should be taken into account for the pharmacological rescue of the patients' mutants
Vehera, Vladyslav. "Přenosové technologie VDSL2 vplus a G.fast a jejich testování." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2020. http://www.nusl.cz/ntk/nusl-413049.
Silva, Dênis [UNESP]. "Purificação e caracterização bioquímica de poligalacturonases produzidas pelo fungo Penicillium viridicatum RFC3 em fermentação em estado sólido e submersa." Universidade Estadual Paulista (UNESP), 2006. http://hdl.handle.net/11449/103949.
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
A poligalacturonase (PG) é uma pectinase que degrada a molécula de pectina atuando internamente , ao acaso, liberando oligossacarídeos (Endo-PG) ou por ataque a partir da extremidade não redutora da cadeia, liberando moléculas de ácido galacturônico (Exo-PG). As pectinases, como várias outras enzimas, têm sido obtidas a partir de microrganismos, em processos fermentativos em estado sólido (FES) e fermentação submersa (FSM). A purificação e caracterização destas enzimas podem possibilitar o estudo das diferenças de suas propriedades funcionais, bem como, permitem avaliar suas possibilidades de aplicação, em diferentes condições, em processos industriais. Este trabalho teve por objetivos estudar a produção, purificação e caracterização da PG produzida por Penicillium viridicatum RFC3 através de FES e FSM. Para FSM foram testados os resíduos agroindustriais (farelo de trigo, bagaço de laranja, uma mistura de ambos) a 3% (p/v) além da água amarela (resíduo do processamento da indústria cítrica) no seu estado bruto e com diluições (2, 4 e 10x). Os pHs iniciais foram corrigidos para 4,5, 5 e 5,5 e a fermentação ocorreu por 24 - 96 h. A ANOVA mostrou que o extrato do bagaço de laranja (pH 5,5 e 96 h) foi o melhor meio para a produção da PG em FSM. A purificação da PG produzida em FSM iniciou-se pela ultrafiltração do extrato bruto (membranas de 50 e 10 kDa, repectivamente) seguida pela aplicação da amostra em uma coluna de filtração em gel Sephadex G75 e posterior aplicação em uma Q Sepharose. A fração isolada e desorvida revelou, após 2 eletroforese (PAGE-SDS) a homogeneidade da banda cuja massa molar foi estimada em 92,24 kDa e pI de 5,4. A enzima mostrou ser uma exopoligalacturonase (Exo-PG) com pH e temperatura ótimos em 5,0 e 50-55ºC.
The polygalacturonase (PG) is a pectinase that degrade pectin acting internally, releasing olygossacharide (Endo-PG) or by atack to a non reducing chain extremity, releasing galacturonic acid (Exo-PG). The pectinases, like many others enzymes, have been obtained from microorganisms by solid state fermentation (SSF) and submerged fermentation (SMF). The purification and characterization of these enzymes besides the study of differences of its functional properties permit its application on industrial process, on different conditions. This work aimed the study of production, purification and characterization of PG from Penicillium viridicatum RFC3 by SSF and SMF. In SMF they were assayed agroindustrial wastes (wheat bran, orange bagasse and a mixture of both) at 3% (w/v 1:1) and citric industry liquid waste (yellow water) in crude state and with dilutions (2, 4 and 10x). It was also evaluated the variation of the initial pH of the medium (4.5, 5.0 nd 5.5) and the time of fermentation (24 96 h). The ANOVA test revealed that the culture medium composed by orange bagasse extract, at pH 5.5 and 96 h, was the best condition for production of PG in SMF. The purification process of PG from SMF was carried out by the ultrafiltration of the crude extract (50 and kDa membranes, respectively) and by gel filtration in Sephadex G75 and Q Sepharose columns. The fraction with PG activity show by eletrophoresis (SDSPAGE), an homogeinity of a band with estimated molar mass of 92.24 KDa and pI 5.4. The enzyme revealed to be an Exo-PG with optimal pH and temperature of 5.0 and 50-55 °C. It also revealed to be Ca2+ dependent, which increased its 4 activity and stability. The Km of the enzyme was 1.30 and 1.16 mg/ml on Ca2+ presence. The Vmax was 1.76 and 2.07 ?mol/min/mg when Ca2+ was added. The better fermentation conditions in SSF was wheat bran mixed with orange bagasse (1:1 w/w) at 80% moisture as an culture medium and 336h of fermentation.
Silva, Dênis. "Purificação e caracterização bioquímica de poligalacturonases produzidas pelo fungo Penicillium viridicatum RFC3 em fermentação em estado sólido e submersa /." Rio Claro : [s.n.], 2006. http://hdl.handle.net/11449/103949.
Banca: Eleonora Cano Carmona
Banca: Rubens Monti
Banca: Iracema de Oliveira Moraes
Banca: Márcia Luzia Rizzatto
Resumo: A poligalacturonase (PG) é uma pectinase que degrada a molécula de pectina atuando internamente, ao acaso, liberando oligossacarídeos (Endo-PG) ou por ataque a partir da extremidade não redutora da cadeia, liberando moléculas de ácido galacturônico (Exo-PG). As pectinases, como várias outras enzimas, têm sido obtidas a partir de microrganismos, em processos fermentativos em estado sólido (FES) e fermentação submersa (FSM). A purificação e caracterização destas enzimas podem possibilitar o estudo das diferenças de suas propriedades funcionais, bem como, permitem avaliar suas possibilidades de aplicação, em diferentes condições, em processos industriais. Este trabalho teve por objetivos estudar a produção, purificação e caracterização da PG produzida por Penicillium viridicatum RFC3 através de FES e FSM. Para FSM foram testados os resíduos agroindustriais (farelo de trigo, bagaço de laranja, uma mistura de ambos) a 3% (p/v) além da água amarela (resíduo do processamento da indústria cítrica) no seu estado bruto e com diluições (2, 4 e 10x). Os pHs iniciais foram corrigidos para 4,5, 5 e 5,5 e a fermentação ocorreu por 24 - 96 h. A ANOVA mostrou que o extrato do bagaço de laranja (pH 5,5 e 96 h) foi o melhor meio para a produção da PG em FSM. A purificação da PG produzida em FSM iniciou-se pela ultrafiltração do extrato bruto (membranas de 50 e 10 kDa, repectivamente) seguida pela aplicação da amostra em uma coluna de filtração em gel Sephadex G75 e posterior aplicação em uma Q Sepharose. A fração isolada e desorvida revelou, após 2 eletroforese (PAGE-SDS) a homogeneidade da banda cuja massa molar foi estimada em 92,24 kDa e pI de 5,4. A enzima mostrou ser uma exopoligalacturonase (Exo-PG) com pH e temperatura ótimos em 5,0 e 50-55ºC
Abstract: The polygalacturonase (PG) is a pectinase that degrade pectin acting internally, releasing olygossacharide (Endo-PG) or by atack to a non reducing chain extremity, releasing galacturonic acid (Exo-PG). The pectinases, like many others enzymes, have been obtained from microorganisms by solid state fermentation (SSF) and submerged fermentation (SMF). The purification and characterization of these enzymes besides the study of differences of its functional properties permit its application on industrial process, on different conditions. This work aimed the study of production, purification and characterization of PG from Penicillium viridicatum RFC3 by SSF and SMF. In SMF they were assayed agroindustrial wastes (wheat bran, orange bagasse and a mixture of both) at 3% (w/v 1:1) and citric industry liquid waste (yellow water) in crude state and with dilutions (2, 4 and 10x). It was also evaluated the variation of the initial pH of the medium (4.5, 5.0 nd 5.5) and the time of fermentation (24 96 h). The ANOVA test revealed that the culture medium composed by orange bagasse extract, at pH 5.5 and 96 h, was the best condition for production of PG in SMF. The purification process of PG from SMF was carried out by the ultrafiltration of the crude extract (50 and kDa membranes, respectively) and by gel filtration in Sephadex G75 and Q Sepharose columns. The fraction with PG activity show by eletrophoresis (SDSPAGE), an homogeinity of a band with estimated molar mass of 92.24 KDa and pI 5.4. The enzyme revealed to be an Exo-PG with optimal pH and temperature of 5.0 and 50-55 °C. It also revealed to be Ca2+ dependent, which increased its 4 activity and stability. The Km of the enzyme was 1.30 and 1.16 mg/ml on Ca2+ presence. The Vmax was 1.76 and 2.07 ?mol/min/mg when Ca2+ was added. The better fermentation conditions in SSF was wheat bran mixed with orange bagasse (1:1 w/w) at 80% moisture as an culture medium and 336h of fermentation
Doutor
Walter, Aaron Joseph. "Approximate Thermal Modeling of Radiofrequency Cardiac Ablation." Diss., CLICK HERE for online access, 2005. http://contentdm.lib.byu.edu/ETD/image/etd1002.pdf.
SILVA, Rafaela Maria Seabra. "Polimorfismos em genes envolvidos no metabolismo do ácido fólico e o risco de desenvolvimento da leucemia infantil." Universidade Federal de Pernambuco, 2010. https://repositorio.ufpe.br/handle/123456789/19894.
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Leucemia é a neoplasia hematológica mais comum na infância. Sua etiologia não está elucidada sendo provável uma interação gene-ambiente. O ácido fólico é uma vitamina do complexo B transportado para a célula pela proteína carreadora de folato reduzido. Enzimas importantes no metabolismo do ácido fólico são: A metilenotetrahidrofolato redutase, metionina sintase, e timidilato sintase. Este foi um estudo exploratório com caso-controle. A população amostral foi diferente para cada um dos polimorfismos estudados sendo constituída de amostras oriundas do CEONHPE/HUOC e INCa. A detecção dos genótipos foi realizada através PCR–RFLP para os polimorfismos MTHFR 677, MS 2756 e RFC1, seguida de digestão enzimática, por PCR para as repetições em tandem no gene TS e PCR alelo-específica para o polimorfismo MTHFR 1298. Não observamos diferença estatisticamente significante na freqüência genotípica dos polimorfismos A2756G MS e nas repetições em tandem 2R/3R no promotor do gene TS. O genótipo mutante RFC 80AA foi relacionado a diminuição do risco de desenvolvimento de leucemias agudas e LLA no CEONHPE, LMA no INCa e leucemias agudas e LMA, na análise CEONHPE e INCa. A presença do alelo 677T foi associada ao aumento do risco de leucemias agudas e LLA. Enquanto a presença do genótipo MTHFR 1298CC foi associado a uma significativa redução do risco. O resultado deste estudo sugere a associação dos polimorfismos RFC G80A, MTHFR A1298C e MTHFR C677T com a susceptibilidade a LA infantil.
Leukemia is the most common hematologic malignancy in childhood. Its etiology is not elucidated and is likely a gene-environment interaction. Folic acid is a vitamin B complex transported to the cell by binding protein of reduced folate. Important enzymes in the metabolism of folic acid are: The methylenetetrahydrofolate reductase, methionine synthase, and thymidylate synthase. This was an exploratory study with case-control. The sample population was different for each of the polymorphisms studied was made up of samples from CEONHPE / HUOC and Cancer Institute. Genotype detection was performed by PCR-RFLP for the polymorphisms MTHFR 677, MS 2756 and RFC1, followed by enzymatic digestion, by PCR for the tandem repeats in the TS gene and allele-specific PCR for the MTHFR 1298 polymorphism. No statistically significant difference in genotype frequency of polymorphisms A2756G MS and tandem repeats in the 2R/3R TS gene promoter. The mutant RFC 80AA genotype was related to decreased risk of developing acute leukemia and in CEONHPE ALL, AML and INCa in acute leukemias and AML, and the analysis CEONHPE Cancer Institute. The presence of 677T allele was associated with increased risk of acute leukemia and ALL. While the presence of the MTHFR 1298CC genotype was associated with a significant reduction in risk. The result of this study suggests the association of polymorphisms G80a RFC, MTHFR A1298C and MTHFR C677T with susceptibility to AL's.
Mancardi, David. "Rôles pro-inflammatoires des RFcγIV murins, et de leurs deux équivalents fonctionnels humains, les RFcγI et les RFcεI(αγ)." Paris 6, 2009. http://www.theses.fr/2009PA066504.
LAUNAY, PIERRE. "Fonctions et signalisations alternatives du rfc alpha dependantes de l'association avec la chaine gamma (doctorat : immunologie)." Paris 5, 1999. http://www.theses.fr/1999PA05N039.
Compaleo, Joshua. "Accurate Clutter Power Modeling Technique for Very LowGrazing Angles with RFC Capable Radar Design and Demonstration." The Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1595563370860726.
Decraene, Maud. "Mise en place de la réponse immunitaire orchestrée par les cellules dendritiques humaines : caractérisation et étude fonctionnelle du rôle des RFcγ." Paris 6, 2005. http://www.theses.fr/2005PA066130.
Ibrahim, Isak Georgina. "Studies on Translation Initiation and Termination in Escherichia coli." Doctoral thesis, Stockholms universitet, Institutionen för genetik, mikrobiologi och toxikologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-69954.
At the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 2: Manuscript.
Grossetête-Pedron, Béatrice. "Role du rfc alpha (cd89) dans la physiopathologie de la maladie de berger et de l'infection a vih." Paris 5, 1999. http://www.theses.fr/1999PA05N115.
Abrisqueta, Costa Pau. "Tratamiento de primera línea con rituximab combinado con fludarabina, ciclofosfamida y mitoxantrone (RFCM) y mantenimiento con rituximab en pacientes con leucemia linfática crónica." Doctoral thesis, Universitat de Barcelona, 2014. http://hdl.handle.net/10803/283529.
Chronic lymphocytic leukemia (CLL) is a frequent malignancy composed of CD5+ B-lymphocytes, is predominant in older people, and has a variable clinical course. The median survival of patients with CLL is approximately 10 years, but the individual prognosis is extremely variable. The addition of monoclonal antibodies to chemotherapy has significantly improved treatment of CLL. Based on excellent results with the chemotherapy-only regimen fludarabine, cyclophosphamide, and mitoxantrone (FCM), we built a new chemoimmunotherapy combination—rituximab plus FCM (R-FCM). The effectiveness of R-FCM followed by rituximab maintenance therapy as first-line treatment for younger patients with CLL (age = 70) has been investigated in a phase 2 clinical trial that included an initial treatment with rituximab 500 mg/m2 on day 1 (375 mg/m2 the first cycle), fludarabine 25 mg/m2 on days 1 to 3, cyclophosphamide 200 mg/m2 on days 1 to 3, and mitoxantrone 6 mg/m2 on day 1 (R-FCM), for 6 cycles. Patients achieving response received maintenance with rituximab 375 mg/m2 every 3 months for 2 years. Eighty-one patients (median age, 60 years; range, 40 to 70 years) were enrolled in the study. The overall response, minimal residual disease (MRD)–negative complete response (CR), MRD positive CR, and partial response (PR) rates were 93%, 46%, 36%, and 11%, respectively. Severe neutropenia developed in 13% of patients. Major and minor infections were reported in 8% and 5% of cycles, respectively. Advanced clinical stage, del(17p), or increased serum ß2-microglobulin levels correlated with a lower CR rate. Sixty-seven patients having achieved CR or PR with R-FCM were given maintenance therapy. At the end of maintenance, 40.6% of patients were in CR MRD-negative, 40.6% were in CR MRD-positive, 4.8% remained in PR, and 14% were considered failures. Six of 29 patients (21%) who were in CR MRD-positive or in PR after R-FCM improved their response upon rituximab maintenance. The 4-year progression-free survival (PFS) and overall survival rates were 74.8% and 93.7%, respectively. MRD status after R-FCM induction was the strongest predictor of PFS. R-FCM is highly effective in previously untreated CLL, with an 82% CR rate and a high proportion of MRD-negative CRs (46%). Treatment toxicity is acceptable. Maintenance with rituximab after R-FCM improved the quality of the response, particularly in patients MRD-positive after initial treatment, and obtained a prolonged PFS.
Díaz, Puentes Alfredo Andrés. "Integración de SAP y Aplicaciones Legadas a Través de SOA." Tesis, Universidad de Chile, 2008. http://repositorio.uchile.cl/handle/2250/104945.
Nieke, Franziska. "Beeinflussung der intestinalen Folatresorption durch Umweltkontaminanten am Beispiel eines humanen in vitro Modells." Doctoral thesis, Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-154886.
Pujol, F. (François). "Experiments on fatty acids chain elongation and glycan flipping in the ER membrane." Doctoral thesis, University of Oulu, 2009. http://urn.fi/urn:isbn:9789514290695.
Veronesi, Junior José Ronaldo. "A eficácia da reeducação postural global através do reequilíbrio funcional corporal (RPG/RFC) na correção postural e no reequilíbrio muscular." reponame:Repositório Institucional da UnB, 2006. http://repositorio.unb.br/handle/10482/5219.
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A presente pesquisa teve como objetivo geral investigar o potencial terapêutico da Reeducação Postural Global através do Reequilíbrio Funcional Laboral RPG/RFL no reequilíbrio muscular e na correção postural em indivíduos hígidos. A amostra foi randomizada e estratificada em dois grupos – controle e tratamento - com 48 participantes cada (n=96). Os testes estatísticos demonstram que a amostra foi homogênea. No grupo tratamento foi realizada uma Reeducação Postural Global através do Reequilíbrio Funcional Corporal, com uma postura inicial e três variantes de membros superiores e seis de membros inferiores durante cinco sessões diárias. O grupo controle não recebeu nenhuma intervenção. Todos os indivíduos foram analisados através de exame de eletromiografia de superfície e fotogrametria computadorizada, antes e após intervalo de cinco dias. No grupo tratamento todos os músculos estudados reequilibraram-se, e no controle todos os músculos desequilibraram-se (p<0,001). No grupo tratamento, 47,92% melhoram todos os segmentos, 41,67% melhoraram dois segmentos, 10,4% melhoraram um segmento apenas. No grupo controle apenas 4,2% melhoraram os três segmentos, 47,92% melhoraram apenas um segmento, e 31,25% não melhoraram nenhum segmento (p<0,001). Estes resultados indicam que o tratamento com o método RPG/RFL foi eficaz no reequilíbrio neuro-muscular e na correção _________________________________________________________________________________________ ABSTRACT
The goal of this research was to investigate the potential therapeutic value of Global Postural Reeducation through re-equilibrium functional labour RPG/RFL on the muscular and the posture correction in normal individuals. The sample was randomized and separated in two groups of 48 participants (n=96). The statistic tests showed that the sample was homogeneous. The treatment group received GPR through Corporal Functional Re-equilibrium, with a first posture and three variants of superior members and six of inferior members during five daily sessions. The control group did not receive any intervention. All participants were examined using surface electromyography and computerized photogrammetry before and after treatment. In the treatment group all muscles investigated showed a re-equilibrium, whereas in the control individuos no a such difference was observed. In the treatment group 47,92% of the individuals improved all segments, 41,67% improved two segments, and 10,4% only one segment. On the other hand, in control group, only 4,2% improved the three segments, 47,92% showed only one segment improved, and 31,25% did not showed any difference. These results indicate that treatment with a Global Posture Reeducation using the Functional labour Re-equilibrium method was efficient it mussel reequilibrium and in the posture correction.
Amigorena, Sebastian. "Activation, proliferation et differenciation des lymphocytes b murins : flux ioniques, expression des rfc gamma de type deux et regulation isotypique." Paris 7, 1990. http://www.theses.fr/1990PA077004.
Martel, Velasquez Victor Ronald. "Diseño de una red de comunicación VPN sobre internet para un Distribuidor Autorizado de Claro basado en el RFC 2764." Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2019. http://hdl.handle.net/10757/625693.
The present project on VPN communication networks over the Internet shows us a guide of necessary configurations by requirements based on RFC 2764 including the functionalities of the network that will allow executing the actions required to streamline business processes through an efficient use of IT resources for the availability of information. Currently companies want to have a presence in new markets, so they expand to new branches in different districts and even in the interior of the country which causes the need to stay connected at all times in a safe, reliable, and especially at a Acceptable cost among all its premises in order to share information that allows them to make decisions in real time. The solution consists of the design of a communication network through virtual private networks between its premises in order to optimize resources and streamline the daily transactions of the business, for which the main focus of the solution is the interconnection of the headquarters principal and its branches, so that various communication solutions between the premises were considered, which will be explained and analyzed. In this analysis we will look for the solution to respond to criteria such as: scalability, data security, availability of information, and above all economy.
Tesis
Ťápal, Tomáš. "Zefektivnění analýzy počítačové sítě 10Gbit/s." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2013. http://www.nusl.cz/ntk/nusl-220194.
Pilčík, Jan. "Metody měření přenosových rychlostí na Internetu." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2008. http://www.nusl.cz/ntk/nusl-217279.
Martin-Verdeaux, Sophie. "Mécanismes moléculaires de l'exocytose mastocytaire médiée par le RFc[epsilon]I : rôle du complexe SNARE et des protéines régulatrices MUNC18 et RAB3D." Paris 5, 2002. http://www.theses.fr/2002PA05P609.
Knoll, Thomas Martin. "BGP Extended Community Attribute for QoS Marking." Universitätsbibliothek Chemnitz, 2008. http://nbn-resolving.de/urn:nbn:de:bsz:ch1-200800767.
Brunati, Silvana. "Régulation de la production des IgG par les lymphocytes T RF#c gamma positifs." Paris 6, 1990. http://www.theses.fr/1990PA066058.
Metz, Josué Claudio. "Avaliação do uso combinado de pó de aciaria elétrica (PAE) com resíduo do forno de cal (RFC) na hidratação de pastas de cimento." Universidade do Vale do Rio dos Sinos, 2016. http://www.repositorio.jesuita.org.br/handle/UNISINOS/5991.
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A concepção de desenvolvimento sustentável tem resultado no aumento de pressões ambientais para a melhoria da eficiência na utilização de recursos, e para a redução das emissões e da geração de resíduos. No processo de fabricação de aço, nas siderúrgicas com fornos elétricos a arco (FEA), ocorre a geração do resíduo sólido denominado pó de aciaria elétrica (PAE). O PAE é constituído por diferentes óxidos metálicos. Contém os elementos químicos cromo (Cr), chumbo (Pb) e cádmio (Cd) e, por isso, é classificado como resíduo classe I – Perigoso pela NBR 10004. Gerado em grandes quantidades diariamente em todo o país, o PAE ainda tem como principal destinação final o aterro industrial. A indústria de papel e celulose, outra atividade de destaque na indústria nacional, gera uma grande quantidade de resíduos em diferentes etapas do processo, entre os quais está o resíduo do forno de cal (RFC), cuja geração está associada a interrupções no funcionamento do forno de cal. A indústria da construção civil, devido a quantidade de matérias-primas consumida e variedade de materiais empregados – cimento, concreto, agregados, cerâmica, entre outros – apresenta-se como alternativa para a reciclagem de resíduos gerados em outros setores da economia. A incorporação do PAE no cimento e concreto produz retardo no tempo de pega em razão da presença de zinco (Zn) no resíduo. Estudos sugerem que em quantidades próximas a 1%, a incorporação de PAE não afeta de forma significativa as propriedades do cimento. O objetivo deste trabalho foi avaliar a influência do uso combinado de PAE e RFC no processo de endurecimento e evolução da hidratação de pastas de cimento. Para tanto, foi realizada a caracterização dos resíduos através dos ensaios de distribuição granulométrica, perda ao fogo, massa específica, área superficial específica, análise química elementar, difração de raios X com refinamento por Rietveld e microscopia eletrônica de varredura. A influência dos resíduos nas pastas de cimento foi avaliada através do ensaio de tempo de pega de acordo com a NBR NM 65 (ABNT, 2003b) e evolução da temperatura semi-adiabática das pastas em estado fresco, além de DRX, com refinamento por Rietveld, e microscopia eletrônica de varredura nas pastas de cimento com diferentes teores de substituição de resíduos (REF, 1%, 2%, 3%, 5% e 10%) em diferentes idades (1, 4 e 7 dias). Os resultados de tempo de pega e evolução da temperatura semi-adiabática indicaram a influência do PAE na hidratação das pastas de cimento. Nas pastas de cimento com substituição de 1% de PAE combinado ou não com RFC, não foram verificados retardos significativos no tempo de pega, demonstrando o potencial do uso de PAE e do RFC na produção de artefatos de cimento.
The sustainable development conception has resulted in increased environmental pressures to improve the efficient use of resources and reduction of emissions and waste generation. In the steelmaking process through electric arc furnaces (EAF), dust generation occurs which is called electric arc furnace dust (EAFD). EAFD is composed by different metal oxides. Some common elements those constitute this dust is chromium, lead and cadmium, and therefore, waste is classified as class I – hazardous by NBR 10004. EAFD has been generated in large quantities every day across the country and it still has mainly landfilled. The pulp and paper industry, another important activity in the domestic industry, generates a lot of waste in different stages of the process, among which is the lime kiln waste (LKW), whose generation is associated with disruptions in the lime oven. Due to expressive amount of raw materials consumed in civil construction industry and also the variety of products available - cement, concrete, aggregates, ceramics, among others - this seems to be an alternative for waste recycling generated in other sectors. The incorporation of EAFD in the cement and concrete produces delayed setting time due to the presence of zinc (Zn) in the waste. Studies suggest that in amounts up to 1%, EAFD incorporation does not affect significantly cement properties. The aim of this work was to evaluate the influence of the combined use of EAFD and LKW in the process of hardening and evolution of the cement pastes hydration. Therefore, were evaluated the characterization of the waste performed through the grain size distribution, loss on ignition, specific gravity, specific surface area, elemental chemical analysis, pH, X ray powder diffraction with refinement by Rietveld and scanning electron microscopy.. The influence of the waste on the cement pastes was evaluated through the setting time tests in accordance with the NBR NM 65 (ABNT, 2003b) and evolution of semi-adiabatic temperature in fresh conditions, XRD with refinement by Rietveld, and SEM in cement pastes with different levels of waste substitution (REF, 1%, 2%, 3%, 5% e 10%) at different ages (1, 4 and 7 days). The setting time results and evolution of semi-adiabatic temperature indicated an increase in the setting time with the increase of the EAFD in cement pastes. In cement pastes with replacement of 1% EAFD combined or not with LKW, significant delays were not verified in the setting time, demonstrating the potential use of EAFD in the production of cement artifacts.
Dutertre, Charles-Antoine. "De l'importance de la caractérisation des différentes isoformes de RFC gamma des cellules NK pour l'optimisation fonctionnelle des anticorps monoclonaux à usage thérapeutique." Paris 7, 2008. http://www.theses.fr/2008PA077132.
Important efforts have been devoted to the optimization of cytotoxic therapeutic monoclonal antibodies (mAb), in particular to the improvement of their capacity to engage the activating RFcγIIIA (CD16) expressed on NK cells that are capable of antibody-dependent cellular cytotoxicity (ADCC). In the present work, we examined the expression of FcγRIl by human NK cells, as well as their role in the control of ADCC, which made it possible to define a novel NK cell subpopulation, CD56dim/CD3ˉ/NKp46⁺/RFcγlllAbright/RFcγllbright that strongly expresses inhibitory FcyRIlB. We showed that FcYRMBbright NK cells, detected in all the donors tested, exhibit an expression profile of NK cell receptors (NKR) different from that of NK RFcγll'°/ˉ cells (that predominantly express the activating FcγRIIC), as well as a reduced degranulation following FcγR-dependent activation. The second part of our work allowed the demonstration that an anti-CD20 EMAB-6 antibody developed by the Laboratoire français du Fractionnement et des Biotechnologies, presents a much higher cytotoxic activity against B cell chronic lymphocytic leukemia (B-CLL) cells in vitro than rituximab, a marketed anti-CD20 therapeutic mAb. This increased activity was linked to a much higher binding capacity to FcyRIIIA as compared to its non-optimized counterpart or to rituximab. Our studies also confirmed that the low fucose level of the EMAB-6 mAb is essential to its increased FcγRIIIA binding and efficacy. Finally, the better cytotoxic activity of this antibody as compared to rituximab was found even increased when the density of CD20 on target cells was lower
Malbec, Odile. "La trans-inhibition : une nouvelle propriété inhibitrice des RFcγIIB." Phd thesis, Université Pierre et Marie Curie - Paris VI, 2012. http://tel.archives-ouvertes.fr/tel-00833016.
Fornůsek, Jan. "Návrh softwarového prostředí pro zpracování dat z měření telekomunikačních sítí." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2012. http://www.nusl.cz/ntk/nusl-219465.
Horváth, Tomáš. "Simulace a měření služeb Triple Play v sítích FTTx." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2013. http://www.nusl.cz/ntk/nusl-220307.
Nwup, Emineimo Kennedy, and Adesola Idris Akande. "Evaluation of the pre IEEE 802.11s RFC : Aspects of the Design and Implementation of the Mesh Station with RA-OLSR in the C-Core." Thesis, Blekinge Tekniska Högskola, Avdelningen för telekommunikationssystem, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-3883.
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