Relevant bibliographies by topics / Retrieval disruption / Journal articles

Journal articles on the topic 'Retrieval disruption'

To see the other types of publications on this topic, follow the link: Retrieval disruption.
Author: Grafiati
Published: 10 December 2022
Last updated: 29 January 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Retrieval disruption.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Kulkumatagi, Savita S. "Secure Data Retrieval for Decentralized Disruption-Tolerent Military Networks." Bonfring International Journal of Software Engineering and Soft Computing 6, Special Issue (October 31, 2016): 223–29. http://dx.doi.org/10.9756/bijsesc.8283.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Dodd, Michael D., Alan D. Castel, and Karen E. Roberts. "A strategy disruption component to retrieval-induced forgetting." Memory & Cognition 34, no. 1 (January 2006): 102–11. http://dx.doi.org/10.3758/bf03193390.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

von der Goltz, C., V. Vengeliene, F. Kiefer, and R. Spanagel. "Pharmacological Disruption of Alcohol-related Memories - Therapeutic Impact of the Theory of Memory Reconsolidation." European Psychiatry 24, S1 (January 2009): 1. http://dx.doi.org/10.1016/s0924-9338(09)70540-1.

Full text
Abstract:
Long-lasting memories that associate environmental stimuli with the effects of alcohol are known to be a main cause of relapse and are a major challenge in the treatment of alcohol addiction. It is reasonable to hypothesize that disrupting consolidated alcohol-related memories might help to prevent relapses. The reconsolidation theory states that a consolidated memory could again become labile and susceptible to disruption by protein synthesis inhibition or NMDA-antagonism after memory retrieval. This has been shown for cocaine- and morphine-associated memories in several recent studies. The aim of our investigations was to examine in an animal model for cue-induced relapse to alcohol-seeking behavior whether the behavioral impact of previously conditioned alcohol associated cues is significantly reduced by blocking the reconsolidation of learned alcohol associations. We show that reconsolidation of alcohol memories is disrupted by post-retrieval ICV-administration of the protein synthesis inhibitor anisomycin. Similarly, post-retrieval i.p.-administration of the NMDA antagonist MK-801 reduced alcohol seeking behavior during the following test day as compared to vehicle treated rats. Pharmacological disruption of reconsolidation of alcohol-associated memories may thus provide a potential therapeutic strategy for the prevention of relapse in alcohol addiction.
APA, Harvard, Vancouver, ISO, and other styles
4

Hur, Junbeom, and Kyungtae Kang. "Secure Data Retrieval for Decentralized Disruption-Tolerant Military Networks." IEEE/ACM Transactions on Networking 22, no. 1 (February 2014): 16–26. http://dx.doi.org/10.1109/tnet.2012.2210729.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Lindsey, Charles D., and H. Shanker Krishnan. "Retrieval Disruption in Collaborative Groups due to Brand Cues." Journal of Consumer Research 33, no. 4 (March 2007): 470–78. http://dx.doi.org/10.1086/510220.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Srihari, B., K. V. G. N. Naidu, and P. Nirupama. "Protect Knowledge Retrieval for Localized Disruption Tolerant Military Networks." International Journal of Computer Trends and Technology 24, no. 3 (June 25, 2015): 108–12. http://dx.doi.org/10.14445/22312803/ijctt-v24p124.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Sai, K., A. Sandeep, and B. Tarakeswara. "Secure Data Retrieval for Decentralized Disruption-Tolerant Military Networks." International Journal of Computer Applications 132, no. 17 (December 17, 2015): 15–17. http://dx.doi.org/10.5120/ijca2015907683.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Kumar, Abhilasha A., and David A. Balota. "Attempted prime retrieval is a double-edged sword: Facilitation and disruption in repeated lexical retrieval." Journal of Experimental Psychology: Learning, Memory, and Cognition 46, no. 8 (August 2020): 1505–32. http://dx.doi.org/10.1037/xlm0000827.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Whalen, John, Michael McCloskey, Ronald P. Lesser, and Barry Gordon. "Localizing Arithmetic Processes in the Brain: Evidence from a Transient Deficit During Cortical Stimulation." Journal of Cognitive Neuroscience 9, no. 3 (May 1997): 409–17. http://dx.doi.org/10.1162/jocn.1997.9.3.409.

Full text
Abstract:
Although substantial progress has been made in characterizing the cognitive processes involved in simple arithmetic, the localization of these processes in the brain is not yet well understood. In this article we consider the localization of a specific arithmetic process, the retrieval of arithmetic table facts from memory. We report a single-patient study in which cortical stimulation was used to create transient disruption of brain activity in localized regions of the cortex. We show that stimulation at a left parietal site impaired performance on simple multiplication problems and further that the impairment reflected stimulation-induced disruption of arithmetic fact retrieval. Our findings support the hypothesis (e.g., Warrington, 1982) that the left parietal lobe is implicated in the arithmetic fact retrieval process.
APA, Harvard, Vancouver, ISO, and other styles
10

Dahlström, Örjan, Henrik Danielsson, Magnus Emilsson, and Jan Andersson. "Does retrieval strategy disruption cause general and specific collaborative inhibition?" Memory 19, no. 2 (February 2011): 140–54. http://dx.doi.org/10.1080/09658211.2010.539571.

Full text
APA, Harvard, Vancouver, ISO, and other styles
11

Barber, Sarah J., and Suparna Rajaram. "Exploring the relationship between retrieval disruption from collaboration and recall." Memory 19, no. 5 (July 2011): 462–69. http://dx.doi.org/10.1080/09658211.2011.584389.

Full text
APA, Harvard, Vancouver, ISO, and other styles
12

Zhu, Liangfu, Qiuji Shao, Tianxiao Li, Jeffrey L. Saver, Li Li, Dujuan Li, Wenli Zhao, and Weijian Jiang. "Evaluation of the JRecan device for thrombus retrieval: efficacy and safety in a swine model of acute arterial occlusion." Journal of NeuroInterventional Surgery 8, no. 5 (May 20, 2015): 526–30. http://dx.doi.org/10.1136/neurintsurg-2015-011721.

Full text
Abstract:
ObjectiveTo evaluate the recanalization efficacy and safety of a novel stent retriever in a swine model of acute arterial occlusion.MethodsThe JRecan thrombectomy device, a stent retriever with a weaving stent design, was evaluated in 18 occluded cervicocerebral vessels of swine. The flow restoration effect immediately upon deployment, the reperfusion rate after retrieval, thromboembolic events, and complications were assessed. The histologic structure of the renal arteries after retriever passage was measured to further assess the safety of JRecan.ResultsImmediate flow restoration was achieved in 66.7% of occlusions (12/18). The reperfusion rate was 94.4% (two Thrombolysis in Cerebral Infarction (TICI) 2b and 15 TICI 3). Distal thromboembolic events did not occur. Microscopic examination of the arteries after retrieval showed mild degrees of endothelial loss in 96.6% (29/30), fibrin or platelet deposition in 53.3% (16/30), and disruption of the internal elastic lamina in 10% (3/30), without severe pathologic lesions.ConclusionsThe JRecan is highly effective at clot removal with a favorable safety profile and merits further development as a stent retriever for the treatment of acute ischemic stroke.
APA, Harvard, Vancouver, ISO, and other styles
13

Bachert, Collin, Tina H. Lee, and Adam D. Linstedt. "Lumenal Endosomal and Golgi-Retrieval Determinants Involved in pH-sensitive Targeting of an Early Golgi Protein." Molecular Biology of the Cell 12, no. 10 (October 2001): 3152–60. http://dx.doi.org/10.1091/mbc.12.10.3152.

Full text
Abstract:
Despite the potential importance of retrieval-based targeting, few Golgi cisternae-localized proteins have been demonstrated to be targeted by retrieval, and the putative retrieval signals remain unknown. Golgi phosphoprotein of 130 kDa (GPP130) is acis-Golgi protein that allows assay of retrieval-based targeting because it redistributes to endosomes upon treatment with agents that disrupt lumenal pH, and it undergoes endosome-to-Golgi retrieval upon drug removal. Analysis of chimeric molecules containing domains from GPP130 and the plasma membrane protein dipeptidylpeptidase IV indicated that GPP130 targeting information is contained entirely within its lumenal domain. Dissection of the lumenal domain indicated that a predicted coiled-coil stem domain adjacent to the transmembrane domain was both required and sufficient for pH-sensitive Golgi localization and endosome-to-Golgi retrieval. Further dissection of this stem domain revealed two noncontiguous stretches that each conferred Golgi localization separated by a stretch that conferred endosomal targeting. Importantly, in the absence of the endosomal determinant the Golgi targeting of constructs containing either or both of the Golgi determinants became insensitive to pH disruption by monensin. Because monensin blocks endosome-to-Golgi transport, the finding that the endosomal determinant confers monensin sensitivity suggests that the endosomal determinant causes GPP130 to traffic to endosomes from which it is normally retrieved. Thus, our observations identify Golgi and endosomal targeting determinants within a lumenal predicted coiled-coil domain that appear to act coordinately to mediate retrieval-based targeting of GPP130.
APA, Harvard, Vancouver, ISO, and other styles
14

G, Saidivya, Rajendra C, and Sreenatha sharma V. "Efficient and Secure Information Retrieval for Decentralized Disruption Tolerant Military Networks." Journal of Applied Physics and Engineering 1, no. 1 (June 30, 2016): 22–25. http://dx.doi.org/10.26524/jap4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
15

Young, Erica J., Massimiliano Aceti, Erica M. Griggs, Rita A. Fuchs, Zachary Zigmond, Gavin Rumbaugh, and Courtney A. Miller. "Selective, Retrieval-Independent Disruption of Methamphetamine-Associated Memory by Actin Depolymerization." Biological Psychiatry 75, no. 2 (January 2014): 96–104. http://dx.doi.org/10.1016/j.biopsych.2013.07.036.

Full text
APA, Harvard, Vancouver, ISO, and other styles
16

von der Goltz, C., V. Vengeliene, F. Kiefer, and R. Spanagel. "Disrupting Reconsolidation of Alcohol Memories Reduces Cue-induced Alcohol Seeking Behavior in Rats." European Psychiatry 24, S1 (January 2009): 1. http://dx.doi.org/10.1016/s0924-9338(09)70705-9.

Full text
Abstract:
In humans alcohol seeking behavior is frequently evoked by the retrieval of memories associated with an alcohol experience. Consolidated memories can become labile if reactivated by reexposure. The aim of our study was to examine whether the behavioral impact of previously conditioned alcohol associated cues is significantly reduced by blocking the reconsolidation of the previously learned alcohol associations that are retrieved by reexposition. For this purpose we applied an animal model for cue-induced relapse to alcohol-seeking behavior. We show that post-retrieval i.p.-administration of 0.1 mg/kg of the NMDA antagonist MK-801 (n=8-10 per group) significantly reduced alcohol seeking behavior during the following test day as compared to vehicle treated animals. Similarly, memory reconsolidation was disrupted by ICV administration of 400 μg of the protein synthesis inhibitor anisomycin (n=9-11 per group). Pharmacological disruption of reconsolidation of alcohol-associated memories may thus provide a potential therapeutic strategy for the prevention of relapse in alcohol addiction.
APA, Harvard, Vancouver, ISO, and other styles
17

Pezzatti, Bergé, Boccard, Codesido, Gagnebin, Viollier, González-Ruiz, and Rudaz. "Choosing an Optimal Sample Preparation in Caulobacter crescentus for Untargeted Metabolomics Approaches." Metabolites 9, no. 10 (September 20, 2019): 193. http://dx.doi.org/10.3390/metabo9100193.

Full text
Abstract:
Untargeted metabolomics aims to provide a global picture of the metabolites present in the system under study. To this end, making a careful choice of sample preparation is mandatory to obtain reliable and reproducible biological information. In this study, eight different sample preparation techniques were evaluated using Caulobacter crescentus as a model for Gram-negative bacteria. Two cell retrieval systems, two quenching and extraction solvents, and two cell disruption procedures were combined in a full factorial experimental design. To fully exploit the multivariate structure of the generated data, the ANOVA multiblock orthogonal partial least squares (AMOPLS) algorithm was employed to decompose the contribution of each factor studied and their potential interactions for a set of annotated metabolites. All main effects of the factors studied were found to have a significant contribution on the total observed variability. Cell retrieval, quenching and extraction solvent, and cell disrupting mechanism accounted respectively for 27.6%, 8.4%, and 7.0% of the total variability. The reproducibility and metabolome coverage of the sample preparation procedures were then compared and evaluated in terms of relative standard deviation (RSD) on the area for the detected metabolites. The protocol showing the best performance in terms of recovery, versatility, and variability was centrifugation for cell retrieval, using MeOH:H2O (8:2) as quenching and extraction solvent, and freeze-thaw cycles as the cell disrupting mechanism.
APA, Harvard, Vancouver, ISO, and other styles
18

Hindy, Nicholas C., Roy Hamilton, Andrea S. Houghtling, H. Branch Coslett, and Sharon L. Thompson-Schill. "Computer-Mouse Tracking Reveals TMS Disruptions of Prefrontal Function During Semantic Retrieval." Journal of Neurophysiology 102, no. 6 (December 2009): 3405–13. http://dx.doi.org/10.1152/jn.00516.2009.

Full text
Abstract:
Converging evidence from neuroimaging and neuropsychological studies is essential for understanding human frontal cortical function. We introduce a new method for studying the effects of transient disruptions of frontal activity during transcranial magnetic stimulation (TMS). Using a novel combination of TMS and computer-mouse tracking, through two experiments we tested process models of semantic competition in left ventrolateral prefrontal cortex (VLPFC). On TMS stimulation of left mid-VLPFC just after presentation of an ambiguous stimulus, participants' mouse-movement trajectories deviated more toward the incorrect target for weak associate trials than for any other trial type. This effect was extinguished when participants were simultaneously shown both target and cue stimuli. Results suggest that left mid-VLPFC is necessary to resolve semantic competition when a response is underdetermined by the stimulus and the interpretive context of the stimulus is ambiguous. Computer-mouse movements reveal the dynamics of competitive interactions as they resolve, making this technique ideally suited for studying cognitive control processes and a more sensitive index of TMS disruption than reaction time and accuracy alone.
APA, Harvard, Vancouver, ISO, and other styles
19

Esparaz, Anthony M., Robert K. Ryu, Ramona Gupta, Scott A. Resnick, Riad Salem, and Robert J. Lewandowski. "Fibrin Cap Disruption: An Adjunctive Technique for Inferior Vena Cava Filter Retrieval." Journal of Vascular and Interventional Radiology 23, no. 9 (September 2012): 1233–35. http://dx.doi.org/10.1016/j.jvir.2012.06.001.

Full text
APA, Harvard, Vancouver, ISO, and other styles
20

Karabulut, M. N., M. Hakan Akay, Ismael A. Salas de Armas, and Igor D. Gregoric. "Emergent valve replacement after circular mapping catheter entrapment in mitral valve apparatus." Interactive CardioVascular and Thoracic Surgery 33, no. 1 (June 12, 2021): 136–38. http://dx.doi.org/10.1093/icvts/ivab042.

Full text
Abstract:
Abstract We present the case of a 47-year-old male patient with a history of symptomatic paroxysmal atrial fibrillation who failed guideline-directed medical treatment and cardioversion. Electrical mapping was attempted and complicated with catheter entrapping in the mitral valve (MV) apparatus, and multiple attempts for retrieval were unsuccessful and caused severe valvular dysfunction. Emergent valve replacement was required secondary to the extensive disruption of the valvular apparatus and posterior annulus. Mapping catheter entrapment in the valvular apparatus is an uncommon complication, but when it occurs, there is a high risk of injury to the MV apparatus during retrieval. To avoid extensive MV damage, operators should use minimal force during standard manoeuvres to extract the catheter. If gentle retrieval manoeuvres fail, surgical exploration and open retrieval may prevent MV damage. Early surgical consultation and exploration could result in open catheter retrieval or MV repair versus MV replacement.
APA, Harvard, Vancouver, ISO, and other styles
21

Giordano, Anthony L., Allan E. Johnson, and Jay S. Rosenblatt. "Haloperidol-induced disruption of retrieval behavior and reversal with apomorphine in lactating rats." Physiology & Behavior 48, no. 1 (July 1990): 211–14. http://dx.doi.org/10.1016/0031-9384(90)90288-f.

Full text
APA, Harvard, Vancouver, ISO, and other styles
22

Verheggen, Inge C. M., Joost J. A. de Jong, Martin P. J. van Boxtel, Alida A. Postma, Jacobus F. A. Jansen, Frans R. J. Verhey, and Walter H. Backes. "Imaging the role of blood–brain barrier disruption in normal cognitive ageing." GeroScience 42, no. 6 (October 6, 2020): 1751–64. http://dx.doi.org/10.1007/s11357-020-00282-1.

Full text
Abstract:
AbstractTo investigate whether blood–brain barrier (BBB) disruption is a potential mechanism of usual age-related cognitive decline, we conducted dynamic contrast–enhanced (DCE) MRI to measure BBB leakage in a healthy sample, and investigated the association with longitudinal cognitive decline. In a sample of neurologically and cognitively healthy, older individuals, BBB leakage rate in the white and grey matter and hippocampus was measured using DCE MRI with pharmacokinetic modelling. Regression analysis was performed to investigate whether the leakage rate was associated with decline in cognitive performance (memory encoding, memory retrieval, executive functioning and processing speed) over 12 years. White and grey matter BBB leakages were significantly associated with decline in memory retrieval. No significant relations were found between hippocampal BBB leakage and cognitive performance. BBB disruption already being associated with usual cognitive ageing, supports that this neurovascular alteration is a possible explanation for the cognitive decline inherent to the ageing process. More insight into BBB leakage during the normal ageing process could improve estimation and interpretation of leakage rate in pathological conditions. The current results might also stimulate the search for strategies to maintain BBB integrity and help increase the proportion people experiencing successful ageing. Netherlands Trial Register number: NL6358, date of registration: 2017-03-24.
APA, Harvard, Vancouver, ISO, and other styles
23

Mendl, M., O. Burman, K. Laughlin, and E. Paul. "Animal Memory and Animal Welfare." Animal Welfare 10, S1 (February 2001): S141—S159. http://dx.doi.org/10.1017/s0962728600023587.

Full text
Abstract:
AbstractRetrieved memories of emotionally laden events are likely to influence the ongoing emotional state and behaviour of animals. If animals consciously experience memories and/or associated emotions, then recall of aversive or pleasurable events will affect their welfare. Even if they do not, retrieval of these (non-conscious/implicit) memories may result in behaviour, such as attempts to escape, that could lead to injury and damage. There is growing evidence that emotionally laden events are more readily stored in memory than neutral ones, and that the neurophysiological basis of this, involving acute elevations of the classic stress hormones and the action of the amygdala, is similar in humans and other vertebrate species. Thus, in humans and animals, emotional memories are likely to be stored as priority information and may readily be retrieved in the presence of relevant cues. If so, an important practical goal is to minimize the chances of negative emotional memories being cued inappropriately, especially for animals in captivity. Disruption of memory formation and retrieval is also important in an animal welfare context. Chronic or very high elevations of stress hormones appear to have both short- and long-term effects on brain structure and function that can interfere with efficient storage of information. Environmental disturbances, including common husbandry procedures, can also disrupt memory formation through retroactive interference effects. Elevated stress levels may both increase the chances of retrieval of negative information while hampering the retrieval of positive or neutral information. These effects may lead to poor learning abilities, selective or disrupted memory retrieval, and consequent inappropriate behaviour with adverse welfare consequences. If we understand them, we may be able to recommend housing or husbandry procedures that minimize the likelihood of their occurrence.
APA, Harvard, Vancouver, ISO, and other styles
24

Ostadi, Bakhtiar, Mohammad Mahdi Seifi, and Ali Husseinzadeh Kashan. "A multi-objective model for resource allocation in disaster situations to enhance the organizational resilience and maximize the value of business continuity with considering events interactions." Proceedings of the Institution of Mechanical Engineers, Part O: Journal of Risk and Reliability 235, no. 5 (February 5, 2021): 814–30. http://dx.doi.org/10.1177/1748006x21991027.

Full text
Abstract:
Industry-related risks are increasing due to the growing expansion of industries. Each of these risks can have a natural origin (such as earthquakes, flood, and fire) or human origin (such as terrorist attacks, contagious diseases, and intentional or inadvertent mistakes of staff). In this regards, business Continuity Management (BCM) is a comprehensive method to identify risks and reduce the consequent impacts on the organizations’ activities. Essential aspects of BCM includes retrieval of products after disruptive events and resource allocation. Obviously, in each organization, the fundamental objective is to allocate the least amount of resources for retrieving operations and minimizing costs, as well as, restoring activities to the tolerance threshold, so that the disruptive events do not stop the critical activities. In this paper, a quantitative model is presented to allocate resources in the shortest possible time capable of minimizing the loss of organizations’ resilience and maximizing the Business Continuity Value (BCV), simultaneously. In case of facing disruption, the interaction of disruptive events and reactive actions is an underlying assumption which is also applied in the current proposed model. After solving the model with a numerical experiment, the results of the model were described, and it was found that disruptive events were recovered before the threshold of tolerance. Also, the results of the model were compared in the two following scenarios: (1) without considering the interaction of disruptive events and (2) with considering the interaction of disruptive events, respectively. In the second scenario, it was found that the activities were later retrieved and the possibility of stop activities was a higher amount.
APA, Harvard, Vancouver, ISO, and other styles
25

Gagnon, Stephanie A., Michael L. Waskom, Thackery I. Brown, and Anthony D. Wagner. "Stress Impairs Episodic Retrieval by Disrupting Hippocampal and Cortical Mechanisms of Remembering." Cerebral Cortex 29, no. 7 (July 27, 2018): 2947–64. http://dx.doi.org/10.1093/cercor/bhy162.

Full text
Abstract:
Abstract Despite decades of science investigating the neural underpinnings of episodic memory retrieval, a critical question remains: how does stress influence remembering and the neural mechanisms of recollection in humans? Here, we used functional magnetic resonance imaging and multivariate pattern analyses to examine the effects of acute stress during retrieval. We report that stress reduced the probability of recollecting the details of past experience, and that this impairment was driven, in part, by a disruption of the relationship between hippocampal activation, cortical reinstatement, and memory performance. Moreover, even memories expressed with high confidence were less accurate under stress, and this stress-induced decline in accuracy was explained by reduced posterior hippocampal engagement despite similar levels of category-level cortical reinstatement. Finally, stress degraded the relationship between the engagement of frontoparietal control networks and retrieval decision uncertainty. Collectively, these findings demonstrate the widespread consequences of acute stress on the neural systems of remembering.
APA, Harvard, Vancouver, ISO, and other styles
26

Dubnau, Josh, Lori Grady, Toshi Kitamoto, and Tim Tully. "Disruption of neurotransmission in Drosophila mushroom body blocks retrieval but not acquisition of memory." Nature 411, no. 6836 (May 2001): 476–80. http://dx.doi.org/10.1038/35078077.

Full text
APA, Harvard, Vancouver, ISO, and other styles
27

Blumen, Helena M., and Suparna Rajaram. "Influence of re-exposure and retrieval disruption during group collaboration on later individual recall." Memory 16, no. 3 (April 2008): 231–44. http://dx.doi.org/10.1080/09658210701804495.

Full text
APA, Harvard, Vancouver, ISO, and other styles
28

Savhira D.L., Risma, Alaika M. Bagus Kurnia PS Alaika, and Dhomirotul Firdaus. "Called Al-Qur’an Digital Perspective; Answer To Raising Hoax In Era Of Disruption." Tribakti: Jurnal Pemikiran Keislaman 31, no. 2 (July 20, 2020): 237–52. http://dx.doi.org/10.33367/tribakti.v31i2.1063.

Full text
Abstract:
Disruption provides a myriad of benefits to people's lives. However, on the other hand it cannot be denied that the era of disruption has brought quite dangerous obstacles. Due to the lack of a person's moral standing in responding to the era of disruption, resulting in various problems arise. One of the big problems born in the era of disruption is the phenomenon of hoaxes. Hoax is triggered by a lack of attention to ethics in communicating with high social media user numbers. The use of this research writing method takes the form of a conceptual framework and is qualitative in nature. Some literature and observations taken from several media issues related to hoax news as data retrieval, and using a phenomenological analysis approach. This paper produces a digital piety concept that can be implemented through tabayyun attitude Qs review. al-H{ujura>t verses 6-8, politeness in language review Qs. T{aha> verse 44, and local wisdom in the language review Qs. al-Hujurat verse 12 obtained from the study of the contents of the Qur'an.
APA, Harvard, Vancouver, ISO, and other styles
29

Elahi, Hajira, Veronica Hong, and Jonathan E. Ploski. "Electroconvulsive Shock Does Not Impair the Reconsolidation of Cued and Contextual Pavlovian Threat Memory." International Journal of Molecular Sciences 21, no. 19 (September 25, 2020): 7072. http://dx.doi.org/10.3390/ijms21197072.

Full text
Abstract:
Existing memories, when retrieved under certain circumstances, can undergo modification through the protein synthesis-dependent process of reconsolidation. Disruption of this process can lead to the weakening of a memory trace, an approach which is being examined as a potential treatment for disorders characterized by pathological memories, such as Post-Traumatic Stress Disorder. The success of this approach relies upon the ability to robustly attenuate reconsolidation; however, the available literature brings into question the reliability of the various drugs used to achieve such a blockade. The identification of a drug or intervention that can reliably disrupt reconsolidation without requiring intracranial access for administration would be extremely useful. Electroconvulsive shock (ECS) delivered after memory retrieval has been demonstrated in some studies to disrupt memory reconsolidation; however, there exists a paucity of literature characterizing its effects on Pavlovian fear memory. Considering this, we chose to examine ECS as an inexpensive and facile means to impair reconsolidation in rats. Here we show that electroconvulsive seizure induction, when administered after memory retrieval, (immediately, after 30 min, or after 1 h), does not impair the reconsolidation of cued or contextual Pavlovian fear memories. On the contrary, ECS administration immediately after extinction training may modestly impair the consolidation of fear extinction memory.
APA, Harvard, Vancouver, ISO, and other styles
30

Putri, Niki Rian. "HUBUNGAN ANTARA PENYALAHGUNAAN JENIS NARKOBA DENGAN GANGGUAN KESEHATAN REPRODUKSI DI LEMBAGA PEMASYARAKATAN KOTA BENGKULU TAHUN 2012." JURNAL MEDIA KESEHATAN 5, no. 2 (November 12, 2018): 119–26. http://dx.doi.org/10.33088/jmk.v5i2.187.

Full text
Abstract:
Drug cases in Bengkulu in 2011 are 246 cases, 6 of 10 abusers in Bengkulu City Correctional Prison have reproductive health disruption. Abuse, among others, may affect the reproductive hormones interfere with testosterone or estrogen hormone metabolism. The aim of this research is to determine the relationship of the drug abuse with reproductive health disorders in Bengkulu City Correctional Prison in 2012. This study uses analytic survey with cross sectional approach. The population of this study are drug abuser in Bengkulu City Prison with 134 samples (total sampling). Retrieval of data by distributing questionnaires then performed univariate analysis with frequency distributions and bivariate analysis with Chi-square.The results of this study indicate that the majority (59.7%) of respondents misuse psychotropic drug, and almost all events (76.2%) of respondents experienced reproductive health disruption. Chi-square test results obtained ρ<0,05, which means there is a significant association between drug abuse with reproductive health disruption with a value of OR = 2.384.Officers are expected to do the treatment for detainees and inmates who have reproductive health disruption by reactivating health care center and doing regular urine tests.
APA, Harvard, Vancouver, ISO, and other styles
31

Putri, Niki Rian. "HUBUNGAN ANTARA PENYALAHGUNAAN JENIS NARKOBA DENGAN GANGGUAN KESEHATAN REPRODUKSI DI LEMBAGA PEMASYARAKATAN KOTA BENGKULU TAHUN 2012." JURNAL MEDIA KESEHATAN 5, no. 2 (November 12, 2018): 119–26. http://dx.doi.org/10.33088/jmk.v5i2.187.

Full text
Abstract:
Drug cases in Bengkulu in 2011 are 246 cases, 6 of 10 abusers in Bengkulu City Correctional Prison have reproductive health disruption. Abuse, among others, may affect the reproductive hormones interfere with testosterone or estrogen hormone metabolism. The aim of this research is to determine the relationship of the drug abuse with reproductive health disorders in Bengkulu City Correctional Prison in 2012. This study uses analytic survey with cross sectional approach. The population of this study are drug abuser in Bengkulu City Prison with 134 samples (total sampling). Retrieval of data by distributing questionnaires then performed univariate analysis with frequency distributions and bivariate analysis with Chi-square.The results of this study indicate that the majority (59.7%) of respondents misuse psychotropic drug, and almost all events (76.2%) of respondents experienced reproductive health disruption. Chi-square test results obtained ρ<0,05, which means there is a significant association between drug abuse with reproductive health disruption with a value of OR = 2.384.Officers are expected to do the treatment for detainees and inmates who have reproductive health disruption by reactivating health care center and doing regular urine tests.
APA, Harvard, Vancouver, ISO, and other styles
32

Martin-Ordas, Gema, and Josep Call. "Memory processing in great apes: the effect of time and sleep." Biology Letters 7, no. 6 (June 2011): 829–32. http://dx.doi.org/10.1098/rsbl.2011.0437.

Full text
Abstract:
Following encoding, memory remains temporarily vulnerable to disruption. Consolidation refers to offline time-dependent processes that continue after encoding and stabilize, transform or enhance the memory trace. Memory consolidation resulting from sleep has been reported for declarative and non-declarative memories in humans. We first investigated the temporal course of memory retrieval in chimpanzees, bonobos and orangutans. We found that the amount of retrieved information was time dependent: apes' performance degraded after 1 and 2 h, stabilized after 4 h, started to increase after 8 and 12 h and fully recovered after 24 h. Second, we show that although memories during wakefulness were highly vulnerable to interference from events similar to those witnessed during the original encoding event, an intervening period of sleep not only stabilized apes' memories into more permanent ones but also protected them against interference.
APA, Harvard, Vancouver, ISO, and other styles
33

Barber, Sarah J., and Suparna Rajaram. "Collaborative memory and part-set cueing impairments: The role of executive depletion in modulating retrieval disruption." Memory 19, no. 4 (May 2011): 378–97. http://dx.doi.org/10.1080/09658211.2011.575787.

Full text
APA, Harvard, Vancouver, ISO, and other styles
34

Katoh, Kazuo. "Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining." International Journal of Cell Biology 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/7076910.

Full text
Abstract:
Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions. Minimizing the incubation time in fixative reduces disruption of tissue morphology, and reducing the incubation time in staining solution or antibody solution decreases nonspecific labeling. Reduction of incubation time in staining solution also decreases the level of background noise. Microwave-assisted tissue preparation is applicable for tissue fixation, decalcification of bone tissues, treatment of adipose tissues, antigen retrieval, and other special staining of tissues. Microwave-assisted tissue fixation and staining are useful tools for histological analyses. This review describes the protocols using microwave irradiation for several essential procedures in histochemical studies, and these techniques are applicable to other protocols for tissue fixation and immunostaining in the field of cell biology.
APA, Harvard, Vancouver, ISO, and other styles
35

Hardwicke, Tom E., Mahdi Taqi, and David R. Shanks. "Postretrieval new learning does not reliably induce human memory updating via reconsolidation." Proceedings of the National Academy of Sciences 113, no. 19 (April 25, 2016): 5206–11. http://dx.doi.org/10.1073/pnas.1601440113.

Full text
Abstract:
Reconsolidation theory proposes that retrieval can destabilize an existing memory trace, opening a time-dependent window during which that trace is amenable to modification. Support for the theory is largely drawn from nonhuman animal studies that use invasive pharmacological or electroconvulsive interventions to disrupt a putative postretrieval restabilization (“reconsolidation”) process. In human reconsolidation studies, however, it is often claimed that postretrieval new learning can be used as a means of “updating” or “rewriting” existing memory traces. This proposal warrants close scrutiny because the ability to modify information stored in the memory system has profound theoretical, clinical, and ethical implications. The present study aimed to replicate and extend a prominent 3-day motor-sequence learning study [Walker MP, Brakefield T, Hobson JA, Stickgold R (2003) Nature 425(6958):616–620] that is widely cited as a convincing demonstration of human reconsolidation. However, in four direct replication attempts (n = 64), we did not observe the critical impairment effect that has previously been taken to indicate disruption of an existing motor memory trace. In three additional conceptual replications (n = 48), we explored the broader validity of reconsolidation-updating theory by using a declarative recall task and sequences similar to phone numbers or computer passwords. Rather than inducing vulnerability to interference, memory retrieval appeared to aid the preservation of existing sequence knowledge relative to a no-retrieval control group. These findings suggest that memory retrieval followed by new learning does not reliably induce human memory updating via reconsolidation.
APA, Harvard, Vancouver, ISO, and other styles
36

Haggbloom, Steven J., and Vickie R. Brewer. "Behavioral disruption versus signal-generated memory retrieval as determinants of the signal-generated partial reinforcement extinction effect." Bulletin of the Psychonomic Society 27, no. 2 (February 1989): 99–102. http://dx.doi.org/10.3758/bf03329908.

Full text
APA, Harvard, Vancouver, ISO, and other styles
37

Offiong, Umoh Bassey, and M. B. Mukesh krishnan. "Securing Data Retrieval for Decentralized Disruption- Tolerant Military Networks (DTNs) using Cipher text- Policy Attribute-Based Encryption." International Journal of Engineering Trends and Technology 26, no. 5 (August 25, 2015): 276–78. http://dx.doi.org/10.14445/22315381/ijett-v26p248.

Full text
APA, Harvard, Vancouver, ISO, and other styles
38

Qi, Lin, Ya P. Liu, Nan N. Zhang, and Ying C. Su. "Predictors of testicular sperm retrieval in patients with non-obstructive azoospermia: a review." Journal of International Medical Research 49, no. 4 (April 2021): 030006052110027. http://dx.doi.org/10.1177/03000605211002703.

Full text
Abstract:
Azoospermia is divided into two categories of obstructive azoospermia and non-obstructive azoospermia. Before 1995, couples with a male partner diagnosed with non-obstructive azoospermia had to choose sperm donation or adoption to have a child. Currently, testicular sperm aspiration or micro-dissection testicular sperm extraction combined with intracytoplasmic sperm injection allows patients with non-obstructive azoospermia to have biological offspring. The sperm retrieval rate is significantly higher in micro-dissection testicular sperm extraction compared with testicular sperm aspiration. Additionally, micro-dissection testicular sperm extraction has the advantages of minimal invasion, safety, limited disruption of testicular function, a low risk of postoperative intratesticular bleeding, and low serum testosterone concentrations. Failed micro-dissection testicular sperm extraction has significant emotional and financial implications on the involved couples. Testicular sperm aspiration and micro-dissection testicular sperm extraction have the possibility of failure. Therefore, predicting the sperm retrieval rate before surgery is important. This narrative review summarizes the existing data on testicular sperm aspiration and micro-dissection testicular sperm extraction to identify the possible factor(s) that can predict the presence of sperm to guide clinical practice. The predictors of surgical sperm retrieval in patients with non-obstructive azoospermia have been widely studied, but there is no consensus.
APA, Harvard, Vancouver, ISO, and other styles
39

Ohlerth, Ann-Katrin, Roelien Bastiaanse, Chiara Negwer, Nico Sollmann, Severin Schramm, Axel Schröder, and Sandro M. Krieg. "Bihemispheric Navigated Transcranial Magnetic Stimulation Mapping for Action Naming Compared to Object Naming in Sentence Context." Brain Sciences 11, no. 9 (September 10, 2021): 1190. http://dx.doi.org/10.3390/brainsci11091190.

Full text
Abstract:
Preoperative language mapping with navigated transcranial magnetic stimulation (nTMS) is currently based on the disruption of performance during object naming. The resulting cortical language maps, however, lack accuracy when compared to intraoperative mapping. The question arises whether nTMS results can be improved, when another language task is considered, involving verb retrieval in sentence context. Twenty healthy German speakers were tested with object naming and a novel action naming task during nTMS language mapping. Error rates and categories in both hemispheres were compared. Action naming showed a significantly higher error rate than object naming in both hemispheres. Error category comparison revealed that this discrepancy stems from more lexico-semantic errors during action naming, indicating lexico-semantic retrieval of the verb being more affected than noun retrieval. In an area-wise comparison, higher error rates surfaced in multiple right-hemisphere areas, but only trends in the left ventral postcentral gyrus and middle superior temporal gyrus. Hesitation errors contributed significantly to the error count, but did not dull the mapping results. Inclusion of action naming coupled with a detailed error analysis may be favorable for nTMS mapping and ultimately improve accuracy in preoperative planning. Moreover, the results stress the recruitment of both left- and right-hemispheric areas during naming.
APA, Harvard, Vancouver, ISO, and other styles
40

Nordahl, Christine Wu, Charan Ranganath, Andrew P. Yonelinas, Charles DeCarli, Evan Fletcher, and William J. Jagust. "White Matter Changes Compromise Prefrontal Cortex Function in Healthy Elderly Individuals." Journal of Cognitive Neuroscience 18, no. 3 (March 1, 2006): 418–29. http://dx.doi.org/10.1162/jocn.2006.18.3.418.

Full text
Abstract:
Changes in memory function in elderly individuals are often attributed to dysfunction of the prefrontal cortex (PFC). One mechanism for this dysfunction may be disruption of white matter tracts that connect the PFC with its anatomical targets. Here, we tested the hypothesis that white matter degeneration is associated with reduced prefrontal activation. We used white matter hyperintensities (WMH), a magnetic resonance imaging (MRI) finding associated with cerebrovascular disease in elderly individuals, as a marker for white matter degeneration. Specifically, we used structural MRI to quantify the extent of WMH in a group of cognitively normal elderly individuals and tested whether these measures were predictive of the magnitude of prefrontal activity (fMRI) observed during performance of an episodic retrieval task and a verbal working memory task. We also examined the effects of WMH located in the dorsolateral frontal regions with the hypothesis that dorsal PFC WMH would be strongly associated with not only PFC function, but also with areas that are anatomically and functionally linked to the PFC in a task-dependent manner. Results showed that increases in both global and regional dorsal PFC WMH volume were associated with decreases in PFC activity. In addition, dorsal PFC WMH volume was associated with decreased activity in medial temporal and anterior cingulate regions during episodic retrieval and decreased activity in the posterior parietal and anterior cingulate cortex during working memory performance. These results suggest that disruption of white matter tracts, especially within the PFC, may be a mechanism for age-related changes in memory functioning.
APA, Harvard, Vancouver, ISO, and other styles
41

Demicheva, E. I., K. Shinwari, K. S. Ushenin, and M. A. Bolkov. "Additional Pathogenic Pathways in RBCK1 Deficiency." Mathematical Biology and Bioinformatics 17, no. 2 (August 9, 2022): 174–87. http://dx.doi.org/10.17537/2022.17.174.

Full text
Abstract:
RBCK1 deficiency is a rare congenital autoinflammatory disease that causes inflammatory disruption on the molecular level. This deficiency has three major clinical manifestations: increased sensitivity to bacterial infections, autoinflammation syndrome, and the accumulation of amylopectin in skeletal muscle. The amylopectinosis causes myopathy and cardiomyopathy. The pathogenesis of the disease is poorly investigated and may include unnoticed relationships. We performed gene expression analysis on patients with RBCK1 deficiency and three other autoinflammatory diseases. The identification of differentially expressed genes revealed a large number of downregulated genes that are involved in the activation of essential metabolic and immune pathways, including NF-kB and Pi3k-Akt-mTOR. Signaling pathways were analysed using the KEGG (Kyoto Encyclopedia of Genes and Genomes) and Gene Ontology resource. Predicted protein-protein interactions were retrieved from the STRING (Search Tool for the Retrieval of Interacting proteins database). Besides the primary involvement of RBCK1 in disease pathology, several downregulated pathways aggravate symptoms of myopathy, cardiomyopathy, and bacterial disease. The studied pathways may serve as new targets for the development of compensatory therapies for patients with RBCK1 deficiency.
APA, Harvard, Vancouver, ISO, and other styles
42

Cohn, Leah A., Melissa R. Stoll, Keith R. Branson, Alice D. Roudabush, Marie E. Kerl, Paige F. Langdon, and Chad M. Johannes. "Fatal Hemothorax Following Management of an Esophageal Foreign Body." Journal of the American Animal Hospital Association 39, no. 3 (May 1, 2003): 251–56. http://dx.doi.org/10.5326/0390251.

Full text
Abstract:
A 10.8-year-old, spayed female toy poodle presented with an esophageal foreign body. The foreign body was removed endoscopically, and a gastrostomy tube was placed to provide nutritional support during esophageal healing. The gastrostomy tube was later removed by endoscopic retrieval of the bulb through the esophagus. Immediately afterward, the dog developed hemothorax and eventually died. It was determined that many small arterial branches were avulsed from the aorta. The involved sections of aorta histopathogically evidenced medial necrosis, which was believed to be related to a prior disruption of blood flow through the vasa vasorum.
APA, Harvard, Vancouver, ISO, and other styles
43

Montefinese, Maria, Glyn Hallam, Hannah Elizabeth Thompson, and Elizabeth Jefferies. "The interplay between control processes and feature relevance: Evidence from dual-task methodology." Quarterly Journal of Experimental Psychology 73, no. 3 (September 25, 2019): 384–95. http://dx.doi.org/10.1177/1747021819877163.

Full text
Abstract:
Neuropsychological studies suggest a distinction between (a) semantic knowledge and (b) control processes that shape the retrieval of conceptual information to suit the task or context. These aspects of semantic cognition are specifically impaired in patients with semantic dementia and semantic aphasia, respectively. However, interactions between the structure of knowledge and control processes that are expected during semantic retrieval have not been fully characterised. In particular, domain-general executive resources may not have equal relevance for the capacity to promote weak yet task relevant features (i.e., “controlled retrieval) and to ignore or suppress distracting information (i.e., “selection”). Here, using a feature selection task, we tested the contribution of featural relevance to semantic performance in healthy participants under conditions of divided attention. Healthy participants showed greater dual-task disruption as the relevance value of the distractor feature linearly increased, supporting the emerging view that semantic relevance is one of the organising principles of the structure of semantic representation. Moreover, word frequency, and inter-correlational strength affected overall performance, but they did not show an interaction with dual-task conditions. These results suggest that domain-general control processes, disrupted by divided attention, are more important to the capacity to efficiently avoid distracting information during semantic decision-making than to the promotion of weak target features. The present study therefore provides novel information about the nature of the interaction between structured conceptual knowledge and control processes that support the retrieval of appropriate information and relates these results to a new theoretical framework, termed controlled semantic cognition.
APA, Harvard, Vancouver, ISO, and other styles
44

Monnat, Jean, Eva M. Neuhaus, Marius S. Pop, David M. Ferrari, Barbara Kramer, and Thierry Soldati. "Identification of a Novel Saturable Endoplasmic Reticulum Localization Mechanism Mediated by the C-Terminus of aDictyostelium Protein Disulfide Isomerase." Molecular Biology of the Cell 11, no. 10 (October 2000): 3469–84. http://dx.doi.org/10.1091/mbc.11.10.3469.

Full text
Abstract:
Localization of soluble endoplasmic reticulum (ER) resident proteins is likely achieved by the complementary action of retrieval and retention mechanisms. Whereas the machinery involving the H/KDEL and related retrieval signals in targeting escapees back to the ER is well characterized, other mechanisms including retention are still poorly understood. We have identified a protein disulfide isomerase (Dd-PDI) lacking the HDEL retrieval signal normally found at the C terminus of ER residents in Dictyostelium discoideum. Here we demonstrate that its 57 residue C-terminal domain is necessary for intracellular retention of Dd-PDI and sufficient to localize a green fluorescent protein (GFP) chimera to the ER, especially to the nuclear envelope. Dd-PDI and GFP-PDI57 are recovered in similar cation-dependent complexes. The overexpression of GFP-PDI57 leads to disruption of endogenous PDI complexes and induces the secretion of PDI, whereas overexpression of a GFP-HDEL chimera induces the secretion of endogenous calreticulin, revealing the presence of two independent and saturable mechanisms. Finally, low-level expression of Dd-PDI but not of PDI truncated of its 57 C-terminal residues complements the otherwise lethal yeast TRG1/PDI1 null mutation, demonstrating functional disulfide isomerase activity and ER localization. Altogether, these results indicate that the PDI57 peptide contains ER localization determinants recognized by a conserved machinery present in D. discoideum and Saccharomyces cerevisiae.
APA, Harvard, Vancouver, ISO, and other styles
45

John, Thomas, and Alp Aslan. "Age differences in the persistence of part-list cuing impairment: The role of retrieval inhibition and strategy disruption." Journal of Experimental Child Psychology 191 (March 2020): 104746. http://dx.doi.org/10.1016/j.jecp.2019.104746.

Full text
APA, Harvard, Vancouver, ISO, and other styles
46

Li, Chao, Huimei Lu, Yong Xiang, and Rui Gao. "Geo-DMP: A DTN-Based Mobile Prototype for Geospatial Data Retrieval." ISPRS International Journal of Geo-Information 9, no. 1 (December 20, 2019): 8. http://dx.doi.org/10.3390/ijgi9010008.

Full text
Abstract:
Geospatial information is gaining immense interest and importance as we enter the era of highly developed transportation and communication. Despite the proliferation of cellular network and WiFi, on some occasions, users still face barriers to accessing geospatial data. In this paper, we design and implement a distributed prototype system with a delay/disruption tolerant network (DTN), named Geo-DMP, for cooperatively and opportunistically sharing and exchanging named geospatial contents in a device-to-device fashion. First of all, we construct a lightweight “content agent” module to bridge the gap between the application layer and the underlying DTN protocol stack. Afterwards, to profile the mobility history of users in practical geospatial environments, we present a map segmentation scheme based on road network and administrative subdivision information. Subsequently, we associate the regional movement history information with the content retrieval process to devise a hierarchical and region-oriented DTN routing scheme for both requests and responses. Finally, we conduct extensive experiments with real-world trajectories and complete implementations on the emulation platform composed of virtual machines. The experiments corroborate that Geo-DMP has the capability of successfully retrieving geospatial contents for users for most of the time under mobile circumstances with episodic connectivity. Moreover, en-route caches can be efficiently exploited to provision contents from multiple sources with less network resource consumption and shorter user-perceived latencies.
APA, Harvard, Vancouver, ISO, and other styles
47

Bernardi, R. E., A. E. Ryabinin, S. P. Berger, and K. M. Lattal. "Post-retrieval disruption of a cocaine conditioned place preference by systemic and intrabasolateral amygdala 2- and 1-adrenergic antagonists." Learning & Memory 16, no. 12 (November 25, 2009): 777–89. http://dx.doi.org/10.1101/lm.1648509.

Full text
APA, Harvard, Vancouver, ISO, and other styles
48

Marion, Stéphanie B., and Craig Thorley. "A meta-analytic review of collaborative inhibition and postcollaborative memory: Testing the predictions of the retrieval strategy disruption hypothesis." Psychological Bulletin 142, no. 11 (November 2016): 1141–64. http://dx.doi.org/10.1037/bul0000071.

Full text
APA, Harvard, Vancouver, ISO, and other styles
49

Cardebat, D., C. Bezy-Vié, J. L. Nespoulous, and M. Puel. "Disruption of retrieval and/or checking-off processes of lexical-phonological representation in confrontation naming by an anomic patient." Journal of Neurolinguistics 5, no. 1 (January 1990): 15–30. http://dx.doi.org/10.1016/0911-6044(90)90029-x.

Full text
APA, Harvard, Vancouver, ISO, and other styles
50

Robinson, Micah, Pak Phi Poon, Christina Schindler, Lois E. Murray, Rachel Kama, Galina Gabriely, Richard A. Singer, Anne Spang, Gerald C. Johnston, and Jeffrey E. Gerst. "The Gcs1 Arf-GAP Mediates Snc1,2 v-SNARE Retrieval to the Golgi in Yeast." Molecular Biology of the Cell 17, no. 4 (April 2006): 1845–58. http://dx.doi.org/10.1091/mbc.e05-09-0832.

Full text
Abstract:
Gcs1 is an Arf GTPase-activating protein (Arf-GAP) that mediates Golgi-ER and post-Golgi vesicle transport in yeast. Here we show that the Snc1,2 v-SNAREs, which mediate endocytosis and exocytosis, interact physically and genetically with Gcs1. Moreover, Gcs1 and the Snc v-SNAREs colocalize to subcellular structures that correspond to the trans-Golgi and endosomal compartments. Studies performed in vitro demonstrate that the Snc-Gcs1 interaction results in the efficient binding of recombinant Arf1Δ17N-Q71L to the v-SNARE and the recruitment of purified coatomer. In contrast, the presence of Snc had no effect on Gcs1 Arf-GAP activity in vitro, suggesting that v-SNARE binding does not attenuate Arf1 function. Disruption of both the SNC and GCS1 genes results in synthetic lethality, whereas overexpression of either SNC gene inhibits the growth of a distinct subset of COPI mutants. We show that GFP-Snc1 recycling to the trans-Golgi is impaired in gcs1Δ cells and these COPI mutants. Together, these results suggest that Gcs1 facilitates the incorporation of the Snc v-SNAREs into COPI recycling vesicles and subsequent endosome-Golgi sorting in yeast.
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography