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Dissertations / Theses on the topic 'Retinoid pathway gene expression'

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Published: 4 June 2021

Last updated: 8 February 2022

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1

Sokolova, Natalia Valerievna. "The role of vitamin A in embryonic lung development in mice." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320687.

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2

Hörnberg, Maria. "Effects of retinoic acid in the mouse olfactory sensory systems /." Umeå : Univ, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1371.

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3

Wang, Xiao Elston Timothy C. "Mathematical modeling of signaling pathway dynamics and stochastic gene expression." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2006. http://dc.lib.unc.edu/u?/etd,367.

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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2006.<br>Title from electronic title page (viewed Oct. 10, 2007). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Statistics and Operations Research." Discipline: Statistics and Operations Research; Department/School: Statistics and Operations Research.

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4

Wright, Alan. "Bayesian pathway analysis in epigenetics." Thesis, University of Plymouth, 2013. http://hdl.handle.net/10026.1/1286.

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A typical gene expression data set consists of measurements of a large number of gene expressions, on a relatively small number of subjects, classified according to two or more outcomes, for example cancer or non-cancer. The identification of associations between gene expressions and outcome is a huge multiple testing problem. Early approaches to this problem involved the application of thousands of univariate tests with corrections for multiplicity. Over the past decade, numerous studies have demonstrated that analyzing gene expression data structured into predefined gene sets can produce ben

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5

Georgiades, Pantelis. "Studies of the expression and function of the retinoid-X-receptor y gene in rodent embryonic development." Thesis, University College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244677.

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6

Livingstone, Julie. "Gene expression and bioinformatics analysis of the isoflavonoid pathway in soybean." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=66992.

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The phenylpropanoid pathway is highly researched due to t he putative nutraceutical benefits of its secondary metabolites. The enzymes of this pathway are member of gene families, but the exact number of gene homologues has to date been unknown. In this study, expressed sequence tags (ESTs) were used to identify all homologues in the isoflavonoid pathway of soybean (Glycine max L. Merr.). Gene expression of all homologues in whole tissues, and at a cellular level in the pod was also investigated using laser capture microdissection (LCM) and real time reverse-transcription pol

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7

Stenico, Verena <1983&gt. "Genus Bifidobacterium: taxonomy studies and gene expression analysis on folate pathway." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amsdottorato.unibo.it/6604/.

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Folates (vitamin B9) are essential water soluble vitamins, whose deficiency in humans may contribute to the onset of several diseases, such as anaemia, cancer, cardiovascular diseases, neurological problems as well as defects in embryonic development. Human and other mammals are unable to synthesize ex novo folate obtaining it from exogenous sources, via intestinal absorption. Recently the gut microbiota has been identified as an important source of folates and the selection and use of folate producing microorganisms represents an innovative strategy to increase human folate levels. The aim

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8

Stelios, Pavlidis. "Pathway based microarray analysis based on multi-membership gene regulation." Thesis, Brunel University, 2012. http://bura.brunel.ac.uk/handle/2438/6968.

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Recent developments in automation and novel experimental techniques have led to the accumulation of vast amounts of biological data and the emergence of numerous databases to store the wealth of information. Consequentially, bioinformatics have drawn considerable attention, accompanied by the development of a plethora of tools for the analysis of biological data. DNA microarrays constitute a prominent example of a high-throughput experimental technique that has required substantial contribution of bioinformatics tools. Following its popularity there is an on-going effort to integrate gene expr

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9

Newton, Sherylanne. "Noise-induced hearing loss : changes in gene expression in the auditory pathway." Thesis, University of Leicester, 2017. http://hdl.handle.net/2381/40895.

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Noise induced hearing loss is classically divided into permanent or temporary forms. Individuals with permanent threshold shifts (PTS) will permanently lose auditory sensitivity, whereas individuals with temporary threshold shifts (TTS) will experience elevated hearing thresholds immediately following noise exposure, which resolves over several weeks. TTS causes little lasting damage to the hair cell, stereocilia or supporting structures which form the organ of Corti within the inner ear, and was therefore considered “no harm, no foul”. However, recent evidence suggests that TTS and PTS also l

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10

Shen, Ying. "Regulation of EphA4 expression through the APC-mediated ubiquitin-proteasome pathway /." View abstract or full-text, 2007. http://library.ust.hk/cgi/db/thesis.pl?BICH%202007%20SHEN.

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11

Chan, Ming Hang (Stanley), and stanley chan@baker edu au. "Investigation of The Intracellular Signalling Pathway for Interleukin-6 Gene Expression in Skeletal Muscle." RMIT University. Medical Sciences, 2007. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080206.091026.

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12

Figueira, Edwin C. Medical Sciences Faculty of Medicine UNSW. "???Stem Cell Pathway??? gene expression in human foetal limbus and cadaveric human limbal epithelium." Awarded by:University of New South Wales. School of Medical Sciences, 2006. http://handle.unsw.edu.au/1959.4/27455.

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Stem cells play a vital role in the turn over of permanently self-renewing tissues (e.g. corneal epithelium, epidermis, bone marrow). Stratified cornea epithelia serve, as ideal tissue models for studying ???stemness???, as the site of cells in the different stages of differentiation are anatomically easily identifiable. Studies targeting limbal stem cell maintenance, in-vitro gene expression during storage and differentiation will benefit future therapeutic applications (e.g. corneal transplantation). Knowledge of stem cell behaviour will help explain the pathophysiology of corneal related oc

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13

Negrete-Urtasun, Susana. "Aspects of the PH signal transduction pathway in the filamentous fungus Aspergillus nidulans." Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286883.

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14

Huang, Norman Jason. "Graph-based Support Vector Machines for Patient Response Prediction Using Pathway and Gene Expression Data." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:11072.

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Over the past decade, multiple function genomic datasets studying chromosomal aberrations and their downstream implications on gene expression have accumulated across a variety of cancer types. With the majority being paired copy number/gene expression profiles originating from the same patient groups, this time frame has also induced a wealth of integrative attempts in hope that the concurrent analysis between both genomic structures will result in optimized downstream results. Borrowing the concept, this dissertation presents a novel contribution to the development of statistical methodology

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15

Brannon, Mark K. "Wnt pathway-mediated transcriptional regulation of the Xenopus dorsoanterior organizing gene siamois /." Thesis, Connect to this title online; UW restricted, 1999. http://hdl.handle.net/1773/9256.

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16

Masserdotti, Giacomo. "ZFP423 coordinates Notch Pathway, Bmp Signaling and EBF/COE transcription factors to regulate Hes5 Gene Expression." Thesis, Open University, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.527455.

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17

Qian, Shuiming. "Study of retrovirus and host interplay: RNA helicase A and microRNA pathway modulate viral gene expression." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1236621870.

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18

Guo, Hong. "Molecular therapy for peritoneal fibrosis targeting the TGF-b/Smad signaling pathway /." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39557509.

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19

Guo, Hong, and 郭紅. "Molecular therapy for peritoneal fibrosis: targeting the TGF-{221}/Smad signaling pathway." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39557509.

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20

Ikram, Mohammed S. "Analysis of Sonic Hedgehog signalling pathway gene expression in Basal Cell Carcinoma and in GLII induced systems." Thesis, Queen Mary, University of London, 2007. http://qmro.qmul.ac.uk/xmlui/handle/123456789/1527.

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Embryonic development is regulated by a number of signalling pathways, which are critical for normal growth. Many of these genes then continue to play an important role in the regulation of cell growth and differentiation in adult. One such pathway is the sonic hedgehog (SHH) pathway; SHH protein is secreted which binds to its receptor patched (PTCH), leading to the activation and repression of target genes via zinc-finger GLI family transcription factors. Deregulation of this pathway, leads to a number of human birth defects and diseases such as Basal Cell Carcinoma (BCC) of the skin. In tran

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21

Ponnampalam, S. N. "A blood-based gene expression and signalling pathway analysis to differentiate between high and low grade gliomas." Thesis, University College London (University of London), 2016. http://discovery.ucl.ac.uk/1485644/.

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Introduction: Brain tumours are the 17th most common cancer worldwide. Gliomas are the most common of the primary brain tumours and are highly malignant. / Objectives: (a) To undertake gene expression profiling of the blood of glioma patients to determine key genetic components of signalling pathways (b) To develop a panel of genes that could be used as a potential blood-based biomarker to differentiate between high and low grade gliomas, non-glioma and control samples. / Methods: Blood samples were obtained from glioma patients, non-glioma and control subjects. Ten samples each were obtained

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22

Jabeen, Mehnaz. "Exploring the cAMP/PKA pathway in the corn smut pathogen Ustilago maydis through serial analysis of gene expression." Thesis, University of British Columbia, 2007. http://hdl.handle.net/2429/31354.

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Ustilago maydis is the causative agent of corn smut disease. The ability of the fungus to switch from budding to filamentous growth is required for pathogenesis. Mating type genes and signaling pathways (cAMP and MAPK) regulate morphogenesis and pathogenesis in U. maydis. A novel protein Hgll appears to be a downstream component of the cAMP pathway that influences cell morphology and sporulation during infection. The focus of this work was to further explore the role of the hgll gene in U. maydis through the construction of Serial Analysis of Gene Expression (SAGE) libraries to compare

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23

Karuppanan, Senniappan S. "The role of MTOR pathway and growth factors as revealed by gene expression profiling in diffuse congenital hyperinsulinism." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1421065/.

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Congenital hyperinsulinism (CHI) is the commonest cause of recurrent and persistent hypoglycaemia in infants. Histologically there are two distinct types: focal and diffuse. The medical therapy for diffuse CHI involves diazoxide, glucagon and octreotide. The patients who are unresponsive to medical therapy require a near total pancreatectomy. However, this often fails to provide the best outcome as some patients continue to have recurrent hypoglycaemia whilst others develop diabetes mellitus in the long term. This study aimed to understand the gene expression pattern in the pancreatic tissues

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24

Batarseh, Amani Musa. "Regulation of translocator protein 18-kDa (TSPO) gene expression through a protein kinase C (PKC) epsilon signal transduction pathway." Connect to Electronic Thesis (CONTENTdm), 2010. http://worldcat.org/oclc/642828809/viewonline.

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25

Ferdous, Amin J. "Cloning and Expression of a Tobacco Stearoyl-ACP Desaturase Gene SBIP24 and its Interaction with SABP2 in SA pathway." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etd/2429.

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Salicylic acid binding protein 2 (SABP2) that converts methyl salicylate to salicylic acid (SA) plays an obligatory role in the SA-mediated disease resistance pathway in plants. SABP2 interacts with SBIP24 in a yeast two-hybrid screening. SBIP24 belongs to the stearoyl-acyl carrier protein-desaturase protein family. To biochemically characterize the SBIP24, it was cloned from tobacco leaves using RT-PCR and expressed in E. coli. Recombinant SBIP24 was affinity purified using Ni-NTA chromatography. RT-PCR was performed to determine the role of SABP2 in modulating the expression SBIP24. TMV infe

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26

Griffitts, Amanda Aline. "Characterization of Host Plant Defense Responses to Parasitization by Orobanche aegyptiaca." Thesis, Virginia Tech, 2001. http://hdl.handle.net/10919/42763.

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<I>Orobanche</I> (spp.) are parasitic plants that attack the roots of many important crops. <I>O. aegyptiaca</I> penetrates the host root (aided by digestive enzymes) and forms connections to the host vascular tissue, from which it will withdraw all of its water and nutrient requirements. In order to control this weed, it is important to understand the relationship between the host and the parasite. To investigate how parasitism effects host defense pathways, we are studying the patterns of expression of host genes known to be involved in various aspects of plant defense responses. With r

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27

Budnjo, Almir. "Gene expression of MAP2K1 and Cyclin D1 in BDII rat model of Endometrial cancer." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-12869.

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Endometrial adenocarcinoma (EAC) is the most frequently diagnosed gynecological cancer of the female genital tract in the Western world. Research studies in EC is difficult to conduct on human tumor samples due to the complex nature of tumor arousal and genetic heterogeneousness in the human population. Therefore, inbred animal models can be promising tools to use in EC research due to similar histopathology and pathogenesis as humans. Studies performed on MAP2K1 and CCND1 has shown that their altered expression play a crucial role in carcinogenesis. CCND1 has been demonstrated to have oncogen

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28

Roberts, Kim G. "Exploration of the ErbB/Ras/MAPK signaling pathway in cancer by gene expression profiling with isoform-specific assay development and microarray technology." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 169 p, 2007. http://proquest.umi.com/pqdweb?did=1362532051&sid=1&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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29

Imbriolo, Jamie. "Increased flux through the hexosamine biosynthetic pathway leads to the induction of acetol-CoA caboxylase gene expression in the heart." Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/21459.

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Thesis (MSc)--Stellenbosch University, 2008.<br>ENGLISH ABSTRACT: Gene expression of the cardiac isoform of acetyl-CoA carboxylase (ACCb) is induced in a glucose-dependent manner. ACCb produces malonyl-CoA, a potent inhibitor of mitochondrial fatty acid uptake. Previous studies show that increased flux through the hexosamine biosynthetic pathway (HBP) under hyperglycaemic conditions may contribute to the development of insulin resistance. In light of this, we hypothesised that increased HBP flux induces cardiac ACCb gene expression thereby contributing to the onset of insulin resistance.

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30

Talerico, Cassandra. "Temporal Activation of the JAK-STAT Pathway in Relation to Cardiac Gene Expression in a Mouse Model of Cardiac Dysfunction." Cleveland State University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=csu1197055735.

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31

Smith, David M. "A role for adenylyl cyclase and the CREB/CRE transctiptional pathway in mammalian behavior /." Thesis, Connect to this title online; UW restricted, 2000. http://hdl.handle.net/1773/6296.

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32

Weir, Kenneth. "A refinement to the semi-quantitative RT-PCR method and defense related, stress resistance and insulin pathway gene expression during Sarcophaga crassipalpis diapause." Diss., Online access via UMI:, 2008.

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33

Ting, Kin Lai. "A calcitonin gene-related peptide-induced signaling pathway directs the synaptic expression of collagen-tail subunit (ColQ) of acetylcholinesterase in muscle /." View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202005%20TING.

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34

Angus, Liselotte. "Willin as a novel 4.1 ezrin radixin moesin (FERM) domain protein in the mammalian Hippo signalling pathway." Thesis, University of St Andrews, 2011. http://hdl.handle.net/10023/2489.

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The Salvador/Warts/Hippo (Hippo) pathway defines a novel signalling cascade regulating cell contact inhibition, organ size control, cell growth, proliferation, apoptosis and cancer development in mammals. The Hippo pathway was initially utilised in D. melanogaster, where the Expanded protein acts in the Hippo signalling cascade to control organ size. Willin is the proposed human orthologue of Expanded and the aim of this thesis is to investigate whether willin can activate the mammalian Hippo signalling pathway. Ectopic willin expression causes an increase in phosphorylation of the core Hippo

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35

Burckin, Todd A. "Probing the integration of steps in the gene expression pathway through analysis of the SPT4-SPT5 transcription elongation complex in Saccharomyces cervisiae /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2004. http://uclibs.org/PID/11984.

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36

Pfister, Anna. "Outcomes of Myosin 1C Gene Expression Depletion on Cancer-related Pathways, in Vitro and in Clinical Samples." Licentiate thesis, Sahlgrenska Academy at University of Gothenburg, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-12981.

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The unconventional myosin IC has previously been suggested to be a haploinsufficient tumour suppressor. The mechanism for this action has hitherto been unknown, however, and hence we decided to attempt to elucidate the genes involved. The first study involved knock-down of MYO1C using siRNA technology followed by whole transcriptiome microarray analysis performed on samples taken at different time points post transfection. This revealed a cornucopia of differential expressions compared to the negative control, among them we found an early up-regulation of the PI3K/AKT pathway and the pathway f

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37

Cheng, Lulu. "Statistical Methods for Genetic Pathway-Based Data Analysis." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/52039.

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The wide application of the genomic microarray technology triggers a tremendous need in the development of the high dimensional genetic data analysis. Many statistical methods for the microarray data analysis consider one gene at a time, but they may miss subtle changes at the single gene level. This limitation may be overcome by considering a set of genes simultaneously where the gene sets are derived from the prior biological knowledge and are called "pathways". We have made contributions on two specific research topics related to the high dimensional genetic pathway data. One is to propose

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38

Wang, Ying. "The role of the hypoxia-inducible factor pathway in bone development and repair." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2007. https://www.mhsl.uab.edu/dt/2009r/wang.pdf.

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39

Bailey, Kathryn Lafaye. "The Use of Microarrays in the Detection of the Gene Expression of Ribulose- 1,5- Bisphosphate Carboxylase/Oxygenase (RubisCO) in the Marine Environment." [Tampa, Fla.] : University of South Florida, 2007. http://purl.fcla.edu/usf/dc/et/SFE0002106.

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40

Rahman-Roblick, Rubaiyat. "The P53 pathway: role of telomerase and identification of novel targets : acts of a master regulator of tumor suppression /." Stockholm, 2007. http://diss.kib.ki.se/2007/978-91-7357-184-5/.

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41

Dean, Phillip T. "Characterization of a Putative Phospholipase D ´ Like Gene as a Lipid Signaling Modulator and Its Role in Salicylic Acid Mediated Defense Pathway in Nicotiana tabacum." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etd/2464.

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Plants are in a perpetual evolutionary arms race with a wide range of pathogens. Their sessile nature has led plants to evolve defense mechanisms that can quickly recognize a unique stressor and deploy a resistance tailored for a specific attack. The salicylic acid (SA) mediated defense pathway has been shown to be one of the major defense tactics plants can initiate to defend themselves against microbial pathogens. Following a pathogen attack high levels of methyl salicylate (MeSA) are produced that can be converted to SA by the enzyme salicylic acid binding protein 2 (SABP2). A yeast two-hyb

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42

Clifton, Rachel. "The alternative oxidase gene family in arabidopsis : insights from a transcriptomic study." University of Western Australia. Biochemistry and Molecular Biology Discipline Group, 2006. http://theses.library.uwa.edu.au/adt-WU2006.0004.

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[Truncated abstract] Mitochondria play an essential role in diverse metabolic pathways in plants. Their primary roles are the oxidation of organic acids via the tricarboxylic acid cycle and the synthesis of ATP coupled to the transfer of electrons from reduced NAD+ to oxygen via the electron transport chain. Plant mitochondria also contain nonphosphorylating bypasses of the respiratory chain, catalysed by the alternative oxidase (AOX), type II NAD(P)H dehydrogenases (NDHs) and uncoupling proteins (UCPs). Each of these components bypasses energy conservation by either circumventing the formatio

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43

Hennes, Thomas Verfasser], Jürgen [Akademischer Betreuer] Bernhagen, and Joost T. van [Akademischer Betreuer] [Dongen. "Gene expression analysis identifies theActivin/Inhibin signaling pathway as a target ofCSN5/JAB1 in colorectal epithelial cancer cells / Thomas Hennes ; Jürgen Bernhagen, Joost Thomas van Dongen." Aachen : Universitätsbibliothek der RWTH Aachen, 2017. http://d-nb.info/1162451289/34.

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May, Patrick, Jan-Ole Christian, Stefan Kempa, and Dirk Walther. "ChlamyCyc : an integrative systems biology database and web-portal for Chlamydomonas reinhardtii." Universität Potsdam, 2009. http://opus.kobv.de/ubp/volltexte/2010/4494/.

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Background: The unicellular green alga Chlamydomonas reinhardtii is an important eukaryotic model organism for the study of photosynthesis and plant growth. In the era of modern highthroughput technologies there is an imperative need to integrate large-scale data sets from highthroughput experimental techniques using computational methods and database resources to provide comprehensive information about the molecular and cellular organization of a single organism. Results: In the framework of the German Systems Biology initiative GoFORSYS, a pathway database and web-portal for Chlamydomonas

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45

Krampis, Konstantinos. "Systems View Of The Soybean Genetic Mechanisms Involved In The Response To Plant Pathogen Infection." Diss., Virginia Tech, 2009. http://hdl.handle.net/10919/37672.

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This thesis involves the important crop plant soybean (Glycine max), and provides a rich information resource for breeders and geneticists working towards improving traits for pathogen resistance.Results reported here provide a systemic view at both the genetic and biochemical level, and were generated by dataÂ-mining gene expression data from soybean cultivars inoculated with plant pathogens and also recombinant inbred line (RIL) populations.The genome variability based on Single Feature Polymorphisms (SFPs) was measured for the first time in soybean, using a genetically diverse set of

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Momin, Amin Altaf. "Application of bioinformatics in studies of sphingolipid biosynthesis." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/34842.

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The studies in this dissertation demonstrate that the gene expression pathway maps are useful tools to notice alteration in different branches of sphingolipid biosynthesis pathway based on microarray and other transcriptomic analysis. To facilitate the integrative analysis of gene expression and sphingolipid amounts, updated pathway maps were prepared using an open access visualization tool, Pathvisio v1.1. The datasets were formatted using Perl scripts and visualized with the aid of color coded pathway diagrams. Comparative analysis of transcriptomics and sphingolipid alterations from experim

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47

Xu, Yaomin. "New Clustering and Feature Selection Procedures with Applications to Gene Microarray Data." Case Western Reserve University School of Graduate Studies / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1196144281.

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Al-Dhaheri, Mariam Hamad. "Molecular Mechanisms of Protein Kinase A Signaling Pathway on Estrogen Receptor Action in Breast Cancer." University of Toledo Health Science Campus / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=mco1174316210.

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49

Luo, Xuebin. "Structural Analysis of the Genes Encoding the Oxalocrotonate Branch of the Pseudomonas putida TOL Plasmid pDKI meta-cleavage Pathway and the Expression of the xy1G Gene Product in Escherichia coli." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc500659/.

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Three overlapping DNA fragments from the lower operon of Pseudomonas putida TOL plasmid pDK1, covering the xy1IH genes and downstream flanking region, were cloned into pUC19. They include a 2.8 kbp XhoI fragment, a 2.7 kbp PstI fragment and a 2.0 kbp EcoRI-HindIII fragment. They were subjected to DNA sequence analysis. The xy1I (4-oxalocrotonate decarboxylase) and xy1H (4-oxalocrotonate tautomerase) genes were found to possess coding regions of 792 and 189 nucleotides, respectively. A possible transcriptional terminator resembling E. coli rho-independent terminators was identified downstream o

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50

Legoedec, Jocelyne. "Le muscle squelettique humain : une source locale de complément. Analyse in vitro de la biosynthèse et de l'activation des protéines du complément par les myoblastes humains." Rouen, 1997. http://www.theses.fr/1997ROUES025.

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Ce mémoire de thèse présente l'étude de l'expression des protéines d'activation et de régulation du système du C par les myoblastes humains normaux et tumoraux en culture. Différentes approches expérimentales ont été utilisées pour l'analyse protéique dans les surnageants de culture (western-blot, marquage biosynthétique et immunoprécipitation, immunofluorescence, dosages ELISA) et pour l'analyse de l'expression des ARNm (RT-PCR). La régulation de cette synthèse par différentes cytokines est envisagée afin d'analyser les variations dans un contexte inflammatoire. Nous montrons que les myoblast

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