Relevant bibliographies by topics / Reproductive techniques – veterinary

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters

Academic literature on the topic 'Reproductive techniques – veterinary'

Author: Grafiati
Published: 29 July 2024
Last updated: 30 July 2024

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Journal articles on the topic "Reproductive techniques – veterinary"

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Igna, Violeta. "Artificial insemination in dog – techniques and associated problems." Practica Veterinara.ro 1, no. 1 (April 19, 2018): 58–65. http://dx.doi.org/10.26416/pv.30.1.2018.1598.

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The requirements of dog owners regarding assisted reproduction have been gradually increasing over the last period of time. Artificial insemination in dog has become a common demand in veterinary clinics nowadays. The practitioner’s knowledge of the canine reproductive physiology and pathology associated with the skills required to perform specific procedures are essential to ensure success and to avoid problems in the use of artificial insemination in dogs. The aim of this paper is to revise the current knowledge about the techniques used in artificial insemination and some problems associated to these techniques.
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Praxedes, Érica Camila Gurgel, Gislayne Christianne Xavier Peixoto, Andréia Maria da Silva, and Alexandre Rodrigues Silva. "Reproduction in agouti (Dasyprocta spp.): A review of reproductive physiology for developing assisted reproductive techniques." Animal Reproduction 15, no. 4 (2018): 1181–92. http://dx.doi.org/10.21451/1984-3143-ar2018-0058.

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Campbell, Madeleine L. H. "Ethics: use and misuse of assisted reproductive techniques across species." Reproduction and Fertility 2, no. 3 (August 2, 2021): C23—C28. http://dx.doi.org/10.1530/raf-21-0004.

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The boundaries of what we are able to do using ARTs are fast-moving. In both human and veterinary medicine, this presents a fundamental question: ‘Just because we can, should we?’ or, to rephrase the same question: ‘How can we distinguish between what is a use and a misuse of an ART, across species?’ This paper assesses the scientific evidence base for and against the use of ARTs and offers a personal opinion on how we can use such evidence to inform an ethical distinction between justifiable and unjustifiable uses of the techniques. It is argued that the law provides a necessary but insufficient basis for such distinctions. Based on the evidence about harms and benefits, ARTs may be classified into three groups: those which should be rarely used; those for which current evidence supports arguments both for and against their use and those which there is an ethical imperative to use. To which category a particular ART falls into varies depending upon the species to which it is being applied and the reason we are using it. In order to ensure that our ethical oversight keeps up with our technical prowess, the medical and veterinary professions should keep discussing and debating the moral basis of the use of ARTs, not only with each other but also with the lay public. Lay summary The use of assisted reproductive techniques (ARTs) has become commonplace in both human and veterinary medicine. Technical limitations are rapidly advancing. This raises a fundamental issue: ‘How can we distinguish between what is a use and a misuse of an ART, across species?’. ‘Misuse’ may be defined both in terms of physical and psychological harms and of moral disquiet about ‘interfering with nature’. This paper assesses the scientific evidence base for and against the use of ARTs and provides a personal opinion on how we can use such evidence to inform an ethical distinction between justifiable and unjustifiable uses of the techniques. We need to consider not only legal but also non-legal ethical justifications for their use. Based on the evidence about harms and benefits, ARTs may be classified into three groups: those which should be rarely used; those for which current evidence supports arguments both for and against their use and those for which there is an ethical imperative to use. To which category a particular ART falls into varies depending upon the species to which it is being applied and the reason we are using it. Open discussion between the medical and veterinary professions and the public is necessary to ensure that ethical oversight of the use of ARTs across species keeps up with technical developments.
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Nagashima, Jennifer B., and Nucharin Songsasen. "Canid Reproductive Biology: Norm and Unique Aspects in Strategies and Mechanisms." Animals 11, no. 3 (March 1, 2021): 653. http://dx.doi.org/10.3390/ani11030653.

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The reproductive physiology of canids is unique compared to other mammalian species. Specifically, the reproductive cycle of female canids is characterized by extended periods of proestrus and estrus followed by obligatory diestrus and protracted ovarian inactivity (anestrus). Although canid reproduction follows this general pattern, studies have shown variations in reproductive biology among species and geographic regions. Understanding of these differences is critical to the development of assisted reproductive technologies including estrus induction, gamete rescue, and embryo production techniques for canid conservation efforts. This review summarizes current knowledge of canid reproduction, including estrus cyclicity, seasonality, and seminal traits, with the emphasis on species diversity. The application of reproductive technologies in wild canid conservation will also be discussed.
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EBERHARDT, MARIA, WOJCIECH NIŻAŃSKI, WANDA OLECH, and SYLWIA PROCHOWSKA. "Assisted reproductive techniques in wisents: Achievements and further challenges." Medycyna Weterynaryjna 77, no. 06 (2021): 279–83. http://dx.doi.org/10.21521/mw.6539.

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The current population of wisents (Bison bonasus, Linnaeus 1758) originates from only 12 individuals that survived World War II in zoos and private enclosures. For better preservation of genetic variability, research is being conducted to introduce assisted reproductive techniques in this species. In this article, we describe the current state of knowledge and prospects for further development in this field. The article is divided into sections dealing with semen collection and preservation, artificial insemination, oocyte collection, in vitro fertilization and embryo transfer, and further challenges.
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Ververs, C., M. Van Zijl Langhout, J. Govaere, and A. Van Soom. "Features of reproduction and assisted reproduction in the white (Ceratotherium simum) and black (Diceros bicornis) rhinoceros." Vlaams Diergeneeskundig Tijdschrift 84, no. 4 (August 31, 2015): 175–87. http://dx.doi.org/10.21825/vdt.v84i4.16593.

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Despite the worldwide increase of rhinoceros calf numbers, the growth of the population of white and black rhinoceros is slowing down mainly due to anthropogenic causes, such as poaching and habitat loss. Assisted reproduction is one of the methods of preserving the valuable genomes of these animals from being lost, and assists in breeding them in captivity to maintain the specie(s) numbers and provide an option for possible reintroduction into the wild. Since wild rhinoceros are difficult to handle and examine clinically, most of the current information available on their reproductive characteristics has been gained from captive rhinoceros populations. Nevertheless, very little is known about rhinoceros reproduction. Since the rhinoceros belongs to the odd-toed ungulates (Perissodactyls) group, like the horse and the tapir, the horse has been proposed as a suitable model to study reproduction and artificial reproductive techniques in the rhinoceros. In this review, the current knowledge of the reproduction of the rhinoceros is summarized.
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Akhtar, Muhammad Faheem, Muhammad Shafiq, and Ilyas Ali. "Improving Gander Reproductive Efficacy in the Context of Globally Sustainable Goose Production." Animals 12, no. 1 (December 27, 2021): 44. http://dx.doi.org/10.3390/ani12010044.

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The goose is a popular poultry species, and in the past two decades the goose industry has become highly profitable across the globe. Ganders low reproductive performance remains a barrier to achieving high fertility and hatchability in subsequent flocks. To address the global demand for cheaper animal protein, various methodologies for improving avian (re)production should be explored. A large amount of literature is available on reproduction traits and techniques for commercial chicken breeder flocks, while research on improved reproduction in ganders has been carried out to a lesser extent. The present review aims to provide a comprehensive literature overview focusing on recent advancements/techniques used in improving gander reproductive efficacy in the context of ensuring a globally sustainable goose industry.
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Kochan, Joanna, Wojciech Niżański, Nei Moreira, Zalmir Silvino Cubas, Agnieszka Nowak, Sylwia Prochowska, Agnieszka Partyka, Wiesława Młodawska, and Józef Skotnicki. "ARTs in wild felid conservation programmes in Poland and in the world." Journal of Veterinary Research 63, no. 3 (September 13, 2019): 457–64. http://dx.doi.org/10.2478/jvetres-2019-0043.

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Abstract With the exception of the domestic cat, all felid species (Felidae) are currently threatened with extinction in their natural habitat. To develop effective and optimal wild cat conservation programmes with assisted reproductive technology (ART) it is necessary to combine advances from different disciplines of science, starting from the biology of the species, through research into the population and habitat, assisted reproductive technologies, establishment of gene banks, developing bioinformatic systems, and ending with biodiversity and endangered species management. In the last few years knowledge of felid reproduction has expanded considerably thanks to comparative studies utilising the domestic cat as a research model for endangered wild cats. Basic reproductive techniques utilised in both domestic cat breeding and rescuing wild felid populations that are threatened with extinction include semen collection and cryopreservation, artificial insemination, oocyte collection, in vitro maturation, in vitro fertilisation, somatic cloning, and embryo transfer. The main directions in which assisted reproductive technologies are being developed in wild cat conservation implementations and the contribution of Polish research centres in advancing these methods are presented.
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O'Connor, C., and A. M. Traas. "Advanced canine reproductive techniques: the most current approaches in breeding management." Journal of Veterinary Behavior 4, no. 6 (November 2009): 256–57. http://dx.doi.org/10.1016/j.jveb.2009.06.012.

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Rekwot, P. I., V. O. Sekoni, S. P. Mallam, E. K. Bawa, Y. U. Abubakar, and C. B. I. Alawa. "Basic principles of nuclear techniques and applications in livestock production." Nigerian Journal of Animal Production 33, no. 1 (January 16, 2021): 128–39. http://dx.doi.org/10.51791/njap.v33i1.2220.

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It is known. that many of the advances which have been made in improving the feeding, fertility and health of livestock in developed countries would not have been possible without nuclear tech-niques. Nuclear techniques have been used by scientists to study and understand those biological processes which are responsible for growth, reproduction and disease resistance in animals. Tracer tech-niques based on isotopes have been used for tagging feed constituents, amino acids and other metabolites, whereby their fate .can be fol-lowed within the digestive system and other parts of the body. This forms an integral part of the armoury used by animal nutritionists to establish the nutri-tional value of all the materials potentially available to feed animals. The development and use of highly sensi­tive immunoassay methods, to measure the minute quantities. of reproductive hormones in the blood enables us to determine when. animals are ready- for breeding, check whether animals have been inseminated at the correct time, diagnose pregnancy early enough, and devise corrective measures for reproductive disorders and improve the efficiency of artificial insemination and embryo transfer programmes. Radioisotopic methods have been used to study the immune system. to examine infec­tious agents and how they Stimulate this system, and to develop specific and sensitive methods for development of veterinary vaccines and diagnostic tests.
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Dissertations / Theses on the topic "Reproductive techniques – veterinary"

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McGee, Marcus. "The use of non-invasive monitoring techniques for profiling hormonal changes associated with stress and reproductive cyclicity in domestic and non-domestic species." Master's thesis, Mississippi State : Mississippi State University, 2009. http://library.msstate.edu/etd/show.asp?etd=etd-02102009-145608.

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Hafez, Shireen Abdelgawad. "Advanced Studies in Veterinary Anatomy: Angiogenesis in Caprine Reproductive Organs of Non-Pregnant and Pregnant Normal and Swainsonine-Treated Does." Diss., Virginia Tech, 2005. http://hdl.handle.net/10919/27091.

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The female reproductive organs are among the few adult tissues in which periodic angiogenesis normally occurs. Pathological angiogenesis can occur in various conditions, such as solid tumors. Vascular endothelial growth factor (VEGF) signaling often represents a critical rate-limiting step in physiological and pathological angiogenesis. This study utilizes development of utero-ovarian vasculature during pregnancy in goats as a model of physiological angiogenesis. Non-pregnant does and does at 4, 7, 10, 13, 16, and 18 weeks of gestation were used. Arteries of the reproductive tract were injected in situ with Microfil®. The tracts were fixed, dehydrated, and rendered transparent to reveal the paths of arteries. The ovarian artery was tortuous and lay in close apposition to the uterine tributary of the ovarian vein in all specimens studied. In non-pregnant does, this arrangement may serve as a local utero-ovarian pathway for the corpus luteum (CL) luteolysis at the end of non-fertile estrous cycle. During pregnancy, this arterio-venous arrangement may transfer luteotropic substances from uterus to ovary, which may serve in maternal recognition of pregnancy and fit the fact that the goat is CL dependent throughout gestation. In some cases of triplets, the size of the uterine branch of the ovarian artery was equal to or even larger than that of its parent artery and/or the ipsilateral uterine artery; and the vaginal artery contributed a connecting branch to the uterine artery. These physiological adaptations of the ovarian and/or vaginal arteries correlate well with the increasing nutrient demands of the growing multiple fetuses. In a second experiment, the vasculature of the uterus and ovaries was injected in situ with a mixture of Batson's No.17® and methyl methacrylate and then processed for observation by SEM. The microvasculature differed between non-pregnant and pregnant does, and with advancing gestation. We concluded that goats possess a multivillous type placenta. Capillary sinusoids and crypts on the fetal surface of the caruncle may compensate for the negative effect of the increased interhemal distance. Intussusceptive angiogenesis should be considered as equally possible and important mechanism as sprouting angiogenesis during placental development. Capillary diameters increased significantly during pregnancy especially after 4 weeks. Capillary density index was 66.8, 68.7, 55.5, 63.5, 70.1, 70.4, 64.5 percent in non-pregnant, 4, 7, 10, 13, 16, and 18 weeks of pregnancy, respectively. In the ovary, coiling of the ovarian branch of the ovarian artery around the ovarian tributary of the ovarian vein was observed. This may represent a local channel required for product transport from ovarian vein to ovarian artery and might have a role in regulating blood pressure to various ovarian structures. Immunolocalization of VEGF was performed as a third experiment. Immunostaining was observed in cyto- trophoblasts, maternal epithelial tissues, and vascular endothelium and smooth muscle, but not in binucleate giant cells or connective tissue. No apparent differences were observed in intensity and pattern of VEGF staining associated with advancing gestation. Luteal and follicular cells, and endothelium and smooth muscles of the ovarian vasculature positively stained. Patterns and intensity of staining of VEGF suggest that the fetus is directing its own survival by producing growth factors affecting fetal and maternal tissues. VEGF may have a role in growth and differentiation of cytotrophoblasts, as well as, development and maintenance of CL. In the fourth experiment, the sequential expression of VEGF and its receptors (fms-like tyrosine kinase, Flt-1 and kinase-insert domain-containing receptor, KDR) was measured using real-time quantitative PCR. Targets were detected in all studied tissues; however, levels of expression differed according to the stage of pregnancy. Expression of VEGF and its receptor mRNAs increased with advancing pregnancy, which correlates with the expansion of vasculature during pregnancy. Differences in the time-courses of the expression of Flt-1 and KDR mRNAs during pregnancy suggest that each receptor plays a different role in the angiogenic process. As an application of our model of angiogenesis, we tested the effect of swainsonine (active compound of locoweed and a potential anti-cancer drug) on the process. Does treated with swainsonine were euthanized at 7 and 18 weeks. No significant differences were found in sinusoidal diameters in treated does at 7 weeks, but a decrease in capillary density index was noted. In the ovary, focal avascular areas were observed in the corpus luteum of swainsonine-treated does at 7 weeks of pregnancy. Swainsonine caused great distortion in the uterine and ovarian vasculature at 18 weeks. A decrease in intensity of the immunoreactivity to VEGF antibody was observed in tissues from swainsonine-treated does at 7 and 18 weeks. There was no substantial effect of swainsonine on the expression of VEGF and its receptors' mRNAs in any of the studied tissues (except in the left ovary, where it had an inhibitory effect) at 7 weeks of pregnancy, but it had an inhibitory effect at 18 weeks. Demonstration of swainsonine's potential to negatively affect vascular development and suppress genes likely involved in angiogenesis at critical stages of blood vessel proliferation lends credibility to its potential as anti-cancer drug.
Ph. D.
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Bas, Santiago. "IMPROVING REPRODUCTIVE PERFORMANCE THROUGH THE DEVELOPMENT OF A DAIRY HERD INDEX, INTRAUTERINE HORMONE DELIVERY OR INSEMINATION TECHNIQUE IN LACTATING COWS." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1365425549.

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Blawut, Bryan Joseph. "Assisted Reproduction Techniques’ Effects on Sperm Physiology of the Freshwater Fish, Sauger (Sander canadensis)." The Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1607012858031537.

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Lamas, Sofia Cristiana dos Santos. "Mouse embryo rederivation and other assisted reproductive techniques and their impact on experimental results." Doctoral thesis, 2021. https://hdl.handle.net/10216/134270.

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Lamas, Sofia Cristiana dos Santos. "Mouse embryo rederivation and other assisted reproductive techniques and their impact on experimental results." Tese, 2021. https://hdl.handle.net/10216/134270.

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Books on the topic "Reproductive techniques – veterinary"

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Heide, Schatten, and Constantinescu Gheorghe M. 1932-, eds. Comparative reproductive biology. Ames, Iowa: Blackwell Pub., 2007.

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Luc, DesCôteaux, Gnemmi Giovanni, and Colloton Jill D, eds. Practical atlas of ruminant and camelid reproductive ultrasonography. Ames, Iowa: Wiley-Blackwell, 2010.

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L, Blanchard Terry, and Blanchard Terry L, eds. Manual of equine reproduction. 2nd ed. St. Louis, Mo: Mosby, 2003.

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P, Brinsko Steven, and Blanchard Terry L, eds. Manual of equine reproduction. 3rd ed. St. Louis, MO: Elsevier/Mosby, 2011.

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Chenoweth, Peter J., and Steven P. Lorton, eds. Manual of animal andrology. Wallingford: CABI, 2022. http://dx.doi.org/10.1079/9781789243505.0000.

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Abstract A succinct reference for those assessing and managing the reproductive functionality of male animals, this practical manual contains both generic and species-specific information suitable for widespread worldwide application. Chapters 1 to 5 covers all relevant aspects such as handling and restraint, physical examination, reproductive examination, important reproductive diseases, biosecurity, semen collection and its assessment, mating behaviour, and the fundamentals of semen handling and preservation for artificial breeding. A simple, concise 'go-to' for the useful techniques and procedures of animal andrology, this book also covers a wide range of species, including cattle (chapter 6), pigs (chapter 7), sheep and goats (chapter 8), camelids (chapter 9), horses (chpater 10), and dogs (chpater 11); provides normal values and ranges for important male reproductive traits, as well as guidelines for breeding soundness evaluations; includes extra supplementary illustrations, protocols and resources through accompanying website to enable further learning. With information presented in a manner that will remain useful for years to come, Manual of Animal Andrology is an essential resource for veterinarians, theriogenologists, animal breeders, and students of veterinary and animal sciences.
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Kustritz, Margaret V. Root. Clinical canine and feline reproduction: Evidence-based answers. Ames, Iowa: Wiley-Blackwell, 2010.

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1932-, Glass Robert H., and Kase Nathan G. 1930-, eds. Clinical gynecologic endocrinology and infertility. 4th ed. Baltimore: Williams & Wilkins, 1989.

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A, Fritz Marc, ed. Clinical gynecologic endocrinology and infertility. 7th ed. Philadelphia: Lippincott Williams & Wilkins, 2005.

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Gygax, Spicer Joan, and Robinson MaryAnne, eds. Managing the environment in critical care nursing. Baltimore: Williams & Wilkins, 1990.

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Speroff, Leon. Clinical gynecologic endocrinology and infertility. 6th ed. Philadelphia: Lippincott Williams & Wilkins, 1999.

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Book chapters on the topic "Reproductive techniques – veterinary"

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Sánchez Dávila, Fernando, and Gerardo Pérez Muñoz. "Reproduction in Small Ruminants (Goats)." In Animal Reproduction in Veterinary Medicine [Working Title]. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.93481.

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The exploitation of small ruminants (goat and sheep) has always been linked to the development of human civilizations, where they have mainly fed on their derived products such as milk and meat. Currently, the sheep population is around 1 billion head concentrated above 50% in three countries, China, Australia, and New Zealand, contrary to goats with around 720 million heads, distributed mainly in Asia, Africa, and South America. Both species have similar characteristics in some anatomical aspects (a pair of nipples), gestation period (150 days), and presence of seasonal anestrus, differing in terms of magnitude and depth and presence of the male effect. However, they are completely different in feeding habits, nutrient needs, and grazing systems, with differences in terms of the female’s reproductive tract, among other characteristics. Currently, the study of reproduction has intensified over the years in the goats and its counterpart that is the buck. Therefore, in the following topics, the importance of global reproduction of the goat will be discussed, considering that progress has been made today in the application of third generation reproductive techniques and that today they are already consolidated and developed in the bovine species.
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S. Aljaser, Feda. "Cryopreservation Methods and Frontiers in the Art of Freezing Life in Animal Models." In Veterinary Medicine and Science. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.101750.

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The development in cryobiology in animal breeding had revolutionized the field of reproductive medicine. The main objective to preserve animal germplasm stems from variety of reasons such as conservation of endangered animal species, animal diversity, and an increased demand of animal models and/or genetically modified animals for research involving animal and human diseases. Cryopreservation has emerged as promising technique for fertility preservation and assisted reproduction techniques (ART) for production of animal breeds and genetically engineered animal species for research. Slow rate freezing and rapid freezing/vitrification are the two main methods of cryopreservation. Slow freezing is characterized by the phase transition (liquid turning into solid) when reducing the temperature below freezing point. Vitrification, on the other hand, is a phenomenon in which liquid solidifies without the formation of ice crystals, thus the process is referred to as a glass transition or ice-free cryopreservation. The vitrification protocol applies high concentrations of cryoprotective agents (CPA) used to avoid cryoinjury. This chapter provides a brief overview of fundamentals of cryopreservation and established methods adopted in cryopreservation. Strategies involved in cryopreserving germ cells (sperm and egg freezing) are included in this chapter. Last section describes the frontiers and advancement of cryopreservation in some of the important animal models like rodents (mouse and rats) and in few large animals (sheep, cow etc).
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Silva Costa Ferreira, Camila, and Rita de Cássia Lima Morais. "Doppler Ultrasound in the Reproduction of Mares." In Veterinary Medicine and Science. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.98951.

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Doppler ultrasonographic (US) is a method that provides real-time information on vascular architecture and hemodynamic aspects of blood vessels. It can determine the presence, direction, and speed of blood flow, being subdivided into the categories of color Doppler (color flow and power flow) and pulsed Doppler. The objective of this chapter was to compile data from several studies addressing the use of US Doppler correlated with pathophysiological phenomena of equine reproduction. Initially we decided to describe the technique, advantages, and disadvantages of each Doppler mode. Then the applicability of US Doppler in mares related to equine reproduction. Thus, within this chapter, you will find the form of use and descriptions of studies carried out on vascular perfusion of the follicular dynamics, the corpus luteum, the uterine segments, which we have divided into post-insemination evaluation, endometritis diagnosis and pregnancy diagnosis. So, we hope that this chapter will expand the knowledge about US Doppler and increase the number of veterinarians who will introduce the technique into their practical routine.
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Lottus Mphaphathi, Masindi, Mamonene Angelinah Thema, Mahlatsana Ramaesela Ledwaba, Maleke Dimpho Sebopela, and Thabang Luther Mashilo. "Perspective Chapter: Wild and Domestic Cats Semen Cryopreservation – Main Aspects and Perspectives." In Veterinary Medicine and Science. IntechOpen, 2024. http://dx.doi.org/10.5772/intechopen.112035.

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Wild and domestic cats can benefit effectively from the use of cryopreserved semen. Different breeding techniques have been applied using cryopreserved semen to produce offspring in wild and domestic cats. To date, the success of these techniques in cats is still inadequate. The majority of sperm tend to lose motility and viability following thawing. Semen must be handled carefully, cryopreserved, and thawed by improving current protocols in order to produce post-thaw sperm of acceptable quality and to result in conception after insemination. The quality of sperm obtained following semen cryopreservation process might be beneficial to the success of reproduction performance. To enhance post-thaw recovery of sperm and fertility, it is crucial to have a thorough understanding of the sperm physiology of the species. These factors include the right diluent, sperm dilution, cooling, and thawing rate. Cryopreservation of semen across cat species often results in poor post-thawed sperm quality, including a decrease in motility and acrosomal integrity of up to 30–50% and a rapid decrease in progressive motility within hours of thawing. Due to the low number of sperm and compromised post-thawed sperm quality, laparoscopic-assisted reproduction is frequently used for artificial insemination procedures in Felids.
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Fernandes Pereira, Alexsandra, Lhara Ricarliany Medeiros de Oliveira, Leonardo Vitorino Costa de Aquino, João Vitor da Silva Viana, and Luanna Lorenna Vieira Rodrigues. "Strategies for the Establishment of Fibroblastic Lines for the Conservation of Wild Mammals." In Veterinary Medicine and Science. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.114028.

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The loss of wild biodiversity has encouraged the development of fibroblastic lines, mainly fibroblasts derived from skin, which can be interesting tools for the conservation of wild mammals. These biological samples, when properly well-established, are essential elements for the reproduction of species through their use in cloning by somatic cell nuclear transfer and induction of cells to pluripotency. In general, the establishment of fibroblastic lines involves the following strategies: (i) cell isolation techniques and identification of fibroblasts; (ii) conditions for in vitro culture of fibroblasts; (iii) conditions for cryopreservation of fibroblasts; and (iv) nuclear reprogramming studies. At each stage, species-specific factors are involved, and determining these lines in the species of interest represents the first step toward its successful use for animal conservation. Therefore, this chapter discusses the stages and parameters involved in the strategies for establishing fibroblastic lines, delving into the main technical aspects and results obtained from the use of these cells in recent years in wild mammals.
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