Relevant bibliographies by topics / Regenerative engineering

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Regenerative engineering'

Author: Grafiati
Published: 6 September 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Regenerative engineering.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Regenerative engineering"

1

Alibardi, Lorenzo. "Regeneration or Scarring Derive from Specific Evolutionary Environmental Adaptations of the Life Cycles in Different Animals." Biology 12, no. 5 (May 17, 2023): 733. http://dx.doi.org/10.3390/biology12050733.

Full text
Abstract:
The ability to heal or even regenerate large injuries in different animals derives from the evolution of their specific life cycles during geological times. The present, new hypothesis tries to explain the distribution of organ regeneration among animals. Only invertebrates and vertebrates that include larval and intense metamorphic transformations can broadly regenerate as adults. Basically, regeneration competent animals are aquatic while terrestrial species have largely or completely lost most of the regeneration ability. Although genomes of terrestrial species still contain numerous genes that in aquatic species allow a broad regeneration (“regenerative genes”), the evolution of terrestrial species has variably modified the genetic networks linking these genes to the others that evolved during land adaptation, resulting in the inhibition of regeneration. Loss of regeneration took place by the elimination of intermediate larval phases and metamorphic transformations in the life cycles of land invertebrates and vertebrates. Once the evolution along a specific lineage generated species that could no longer regenerate, this outcome could not change anymore. It is therefore likely that what we learn from regenerative species will explain their mechanisms of regeneration but cannot or only partly be applied to non-regenerative species. Attempts to introduce “regenerative genes” in non-regenerative species most likely would disorder the entire genetic networks of the latter, determining death, teratomas and cancer. This awareness indicates the difficulty to introduce regenerative genes and their activation pathways in species that evolved genetic networks suppressing organ regeneration. Organ regeneration in non-regenerating animals such as humans should move to bio-engineering interventions in addition to “localized regenerative gene therapies” in order to replace lost tissues or organs.
APA, Harvard, Vancouver, ISO, and other styles
2

Esdaille, Caldon J., Kenyatta S. Washington, and Cato T. Laurencin. "Regenerative engineering: a review of recent advances and future directions." Regenerative Medicine 16, no. 5 (May 2021): 495–512. http://dx.doi.org/10.2217/rme-2021-0016.

Full text
Abstract:
Regenerative engineering is defined as the convergence of the disciplines of advanced material science, stem cell science, physics, developmental biology and clinical translation for the regeneration of complex tissues and organ systems. It is an expansion of tissue engineering, which was first developed as a method of repair and restoration of human tissue. In the past three decades, advances in regenerative engineering have made it possible to treat a variety of clinical challenges by utilizing cutting-edge technology currently available to harness the body’s healing and regenerative abilities. The emergence of new information in developmental biology, stem cell science, advanced material science and nanotechnology have provided promising concepts and approaches to regenerate complex tissues and structures.
APA, Harvard, Vancouver, ISO, and other styles
3

Laurencin, Cato, and Naveen Nagiah. "Regenerative Engineering-The Convergence Quest." MRS Advances 3, no. 30 (2018): 1665–70. http://dx.doi.org/10.1557/adv.2018.56.

Full text
Abstract:
ABSTRACTWe define Regenerative Engineering as a Convergence of Advanced Materials Science, Stem Cell Science, Physics, Developmental Biology, and Clinical Translation. We believe that an “un-siloed’ technology approach will be important in the future to realize grand challenges such as limb and organ regeneration. We also believe that biomaterials will play a key role in achieving overall translational goals. Through convergence of a number of technologies, with advanced materials science playing an important role, we believe the prospect of engaging future grand challenges is possible. Regenerative Engineering as a field is particularly suited for solving clinical problems that are relevant today. The paradigms utilized can be applied to the regeneration of tissue in the shoulder where tendon and muscle currently have low levels of regenerative capability, and the consequences, especially in alternative surgical solutions for massive tendon and muscle loss at the shoulder have demonstrated significant morbidity. Polymer, polymer-cell, and polymer biological factor, and polymer-physical systems can be utilized to propose a range of solutions to shoulder tissue regeneration. The approaches, possibilities, limitations and future strategies, allow for a variety of clinical solutions in musculoskeletal disease treatment.
APA, Harvard, Vancouver, ISO, and other styles
4

Li, Yan, Lungen Lu, and Xiaobo Cai. "Liver Regeneration and Cell Transplantation for End-Stage Liver Disease." Biomolecules 11, no. 12 (December 20, 2021): 1907. http://dx.doi.org/10.3390/biom11121907.

Full text
Abstract:
Liver transplantation is the only curative option for end-stage liver disease; however, the limitations of liver transplantation require further research into other alternatives. Considering that liver regeneration is prevalent in liver injury settings, regenerative medicine is suggested as a promising therapeutic strategy for end-stage liver disease. Upon the source of regenerating hepatocytes, liver regeneration could be divided into two categories: hepatocyte-driven liver regeneration (typical regeneration) and liver progenitor cell-driven liver regeneration (alternative regeneration). Due to the massive loss of hepatocytes, the alternative regeneration plays a vital role in end-stage liver disease. Advances in knowledge of liver regeneration and tissue engineering have accelerated the progress of regenerative medicine strategies for end-stage liver disease. In this article, we generally reviewed the recent findings and current knowledge of liver regeneration, mainly regarding aspects of the histological basis of regeneration, histogenesis and mechanisms of hepatocytes’ regeneration. In addition, this review provides an update on the regenerative medicine strategies for end-stage liver disease. We conclude that regenerative medicine is a promising therapeutic strategy for end-stage liver disease. However, further studies are still required.
APA, Harvard, Vancouver, ISO, and other styles
5

Laurencin, C. T., and Y. Khan. "Regenerative Engineering." Science Translational Medicine 4, no. 160 (November 14, 2012): 160ed9. http://dx.doi.org/10.1126/scitranslmed.3004467.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Wu, David T., Jose G. Munguia-Lopez, Ye Won Cho, Xiaolu Ma, Vivian Song, Zhiyue Zhu, and Simon D. Tran. "Polymeric Scaffolds for Dental, Oral, and Craniofacial Regenerative Medicine." Molecules 26, no. 22 (November 22, 2021): 7043. http://dx.doi.org/10.3390/molecules26227043.

Full text
Abstract:
Dental, oral, and craniofacial (DOC) regenerative medicine aims to repair or regenerate DOC tissues including teeth, dental pulp, periodontal tissues, salivary gland, temporomandibular joint (TMJ), hard (bone, cartilage), and soft (muscle, nerve, skin) tissues of the craniofacial complex. Polymeric materials have a broad range of applications in biomedical engineering and regenerative medicine functioning as tissue engineering scaffolds, carriers for cell-based therapies, and biomedical devices for delivery of drugs and biologics. The focus of this review is to discuss the properties and clinical indications of polymeric scaffold materials and extracellular matrix technologies for DOC regenerative medicine. More specifically, this review outlines the key properties, advantages and drawbacks of natural polymers including alginate, cellulose, chitosan, silk, collagen, gelatin, fibrin, laminin, decellularized extracellular matrix, and hyaluronic acid, as well as synthetic polymers including polylactic acid (PLA), polyglycolic acid (PGA), polycaprolactone (PCL), poly (ethylene glycol) (PEG), and Zwitterionic polymers. This review highlights key clinical applications of polymeric scaffolding materials to repair and/or regenerate various DOC tissues. Particularly, polymeric materials used in clinical procedures are discussed including alveolar ridge preservation, vertical and horizontal ridge augmentation, maxillary sinus augmentation, TMJ reconstruction, periodontal regeneration, periodontal/peri-implant plastic surgery, regenerative endodontics. In addition, polymeric scaffolds application in whole tooth and salivary gland regeneration are discussed.
APA, Harvard, Vancouver, ISO, and other styles
7

Hosseini, F. S., L. S. Nair, and C. T. Laurencin. "Inductive Materials for Regenerative Engineering." Journal of Dental Research 100, no. 10 (April 27, 2021): 1011–19. http://dx.doi.org/10.1177/00220345211010436.

Full text
Abstract:
Regenerative engineering has pioneered several novel biomaterials to treat critical-sized bone injuries. However, despite significant improvement in synthetic materials research, some limitations still exist. The constraints correlated with the current grafting methods signify a treatment paradigm shift to osteoinductive regenerative engineering approaches. Because of their intrinsic potential, inductive biomaterials may represent alternative approaches to treating critical bone injuries. Osteoinductive scaffolds stimulate stem cell differentiation into the osteoblastic lineage, enhancing bone regeneration. Inductive biomaterials comprise polymers, calcium phosphate ceramics, metals, and graphene family materials. This review will assess the cellular behavior toward properties of inductive materials.
APA, Harvard, Vancouver, ISO, and other styles
8

Dzobo, Kevin, Nicholas Ekow Thomford, Dimakatso Alice Senthebane, Hendrina Shipanga, Arielle Rowe, Collet Dandara, Michael Pillay, and Keolebogile Shirley Caroline M. Motaung. "Advances in Regenerative Medicine and Tissue Engineering: Innovation and Transformation of Medicine." Stem Cells International 2018 (July 30, 2018): 1–24. http://dx.doi.org/10.1155/2018/2495848.

Full text
Abstract:
Humans and animals lose tissues and organs due to congenital defects, trauma, and diseases. The human body has a low regenerative potential as opposed to the urodele amphibians commonly referred to as salamanders. Globally, millions of people would benefit immensely if tissues and organs can be replaced on demand. Traditionally, transplantation of intact tissues and organs has been the bedrock to replace damaged and diseased parts of the body. The sole reliance on transplantation has created a waiting list of people requiring donated tissues and organs, and generally, supply cannot meet the demand. The total cost to society in terms of caring for patients with failing organs and debilitating diseases is enormous. Scientists and clinicians, motivated by the need to develop safe and reliable sources of tissues and organs, have been improving therapies and technologies that can regenerate tissues and in some cases create new tissues altogether. Tissue engineering and/or regenerative medicine are fields of life science employing both engineering and biological principles to create new tissues and organs and to promote the regeneration of damaged or diseased tissues and organs. Major advances and innovations are being made in the fields of tissue engineering and regenerative medicine and have a huge impact on three-dimensional bioprinting (3D bioprinting) of tissues and organs. 3D bioprinting holds great promise for artificial tissue and organ bioprinting, thereby revolutionizing the field of regenerative medicine. This review discusses how recent advances in the field of regenerative medicine and tissue engineering can improve 3D bioprinting and vice versa. Several challenges must be overcome in the application of 3D bioprinting before this disruptive technology is widely used to create organotypic constructs for regenerative medicine.
APA, Harvard, Vancouver, ISO, and other styles
9

Laurencin, Cato T., and Roshan James. "Composites and Structures for Regenerative Engineering." MRS Proceedings 1621 (2014): 3–15. http://dx.doi.org/10.1557/opl.2014.4.

Full text
Abstract:
ABSTRACTRegenerative engineering was conceptualized by bridging the lessons learned in developmental biology and stem cell science with biomaterial constructs and engineering principles to ultimately generate de novo tissue. We seek to incorporate our understanding of natural tissue development to design tissue-inducing biomaterials, structures and composites than can stimulate the regeneration of complex tissues, organs, and organ systems through location-specific topographies and physico-chemical cues incorporated into a continuous phase. This combination of classical top-down tissue engineering approach with bottom-up strategies used in regenerative biology represents a new multidisciplinary paradigm. Advanced surface topographies and material scales are used to control cell fate and the consequent regenerative capacity.Musculoskeletal tissues are critical to the normal functioning of an individual and following damage or degeneration they show extremely limited endogenous regenerative capacity. The increasing demand for biologically compatible donor tissue and organ transplants far outstrips the availability leading to an acute shortage. We have developed several biomimetic structures using various biomaterial platforms to combine optimal mechanical properties, porosity, bioactivity, and functionality to effect repair and regeneration of hard tissues such as bone, and soft tissues such as ligament and tendon. Starting with simple structures, we have developed composite and multi-scale systems that very closely mimic the native tissue architecture and material composition. Ultimately, we aim to modulate the regenerative potential, including proliferation, phenotype maturation, matrix production, and apoptosis through cell-scaffold and host –scaffold interactions developing complex tissues and organ systems.
APA, Harvard, Vancouver, ISO, and other styles
10

McInnes, Adam D., Michael A. J. Moser, and Xiongbiao Chen. "Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering." Journal of Functional Biomaterials 13, no. 4 (November 14, 2022): 240. http://dx.doi.org/10.3390/jfb13040240.

Full text
Abstract:
The multidisciplinary fields of tissue engineering and regenerative medicine have the potential to revolutionize the practise of medicine through the abilities to repair, regenerate, or replace tissues and organs with functional engineered constructs. To this end, tissue engineering combines scaffolding materials with cells and biologically active molecules into constructs with the appropriate structures and properties for tissue/organ regeneration, where scaffolding materials and biomolecules are the keys to mimic the native extracellular matrix (ECM). For this, one emerging way is to decellularize the native ECM into the materials suitable for, directly or in combination with other materials, creating functional constructs. Over the past decade, decellularized ECM (or dECM) has greatly facilitated the advance of tissue engineering and regenerative medicine, while being challenged in many ways. This article reviews the recent development of dECM for tissue engineering and regenerative medicine, with a focus on the preparation of dECM along with its influence on cell culture, the modification of dECM for use as a scaffolding material, and the novel techniques and emerging trends in processing dECM into functional constructs. We highlight the success of dECM and constructs in the in vitro, in vivo, and clinical applications and further identify the key issues and challenges involved, along with a discussion of future research directions.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Regenerative engineering"

1

Tan, Richard Philip. "Developing Translational Tissue Engineering Solutions for Regenerative Medicine." Thesis, The University of Sydney, 2018. http://hdl.handle.net/2123/20200.

Full text
Abstract:
Regenerative medicine is an emerging field that aims to treat injury and disease by harnessing and augmenting the body’s innate capacity for tissue regeneration. Many of the strategies developed in this field have relied extensively on the principles of tissue engineering, a set of methods that bring together cells, cellular signals and material scaffolds to repair or replace biological tissue. While the number of novel tissue engineering strategies continues to rapidly expand, the innovations underlying these solutions often fail to consider the key technical, manufacturing, and regulatory barriers that prohibit these technologies from suitable use in humans. As a result, the field of tissue engineering has one of the lowest rates of clinical translation amongst medical research. To address this, this thesis examines each of the prominent components of the tissue engineering practice and develops tools and strategies that enable the development of solutions with high translational potential. The collective findings of these works propose tools and solutions applicable within the major facets of tissue engineering that may help to lay the groundwork for future therapies with high clinical probability in a number of regenerative medicine applications.
APA, Harvard, Vancouver, ISO, and other styles
2

George, Julian H. S. "Engineering of fibrous scaffolds for use in regenerative medicine." Thesis, Imperial College London, 2009. http://hdl.handle.net/10044/1/5298.

Full text
Abstract:
Tissue engineering with fibrous scaffolds is emerging as a major research area in the field of regenerative medicine. The major themes pursued in this thesis are the study of the cellular response to nanofibrous constructs, the role of nanofibres in the engineering of synthetic scaffolds, and the development of technology to facilitate the fabrication of nanofibrous scaffolds with controlled architectures. Cells cultured on multi‐walled carbon nanotubes displayed reduced proliferation and altered cytoskeletal development, thought to be due to the undermining of the maturation of focal adhesions. Development of an electrospinning chamber enabled the creation of poly(methyl methacrylate), poly(lactic acid) and poly(caprolactone) fibres for the study of cellular response to nano‐ and macro‐fibrous scaffolds. Cell attachment and organisation on the electrospun fibres was visualised using scanning electron microscopy, oblique microscopy and live cell microscopy. It was found that the incorporation of nanofibres into scaffolds restricts the maturation of focal adhesions which modulates cytoskeletal formation. This can be used to restrict the migration and the proliferation of attachment dependant cells such as osteoblasts or maintain the differentiation of cells such as chodrocytes. To scale up electrospun fibre production, use of rotating collectors, multi‐jet spinning and secondary electrodes to focus the spinning were investigated. Further to this, development of an array of focusing electrodes to control, stabilise and deflect the jet was also investigated towards the creation of a rapid‐prototype electrospinning system. The secondary electrode array was found to reduce the spreading of the jet to a spot diameter of 10mm and charged deflection plates successfully redirecting the position of the jet as it arrived at the collector.
APA, Harvard, Vancouver, ISO, and other styles
3

Gebhardt, Matthew. "Evaluation of tissue engineering scaffolds for regenerative endodontic treatment." Thesis, NSUWorks, 2008. https://nsuworks.nova.edu/hpd_cdm_stuetd/10.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Speccher, Alessandra. "Tissue engineering approaches for brain injury applications." Doctoral thesis, Università degli studi di Trento, 2020. http://hdl.handle.net/11572/262798.

Full text
Abstract:
Due to the limited regenerative capacity of the central nervous system (CNS) upon injury, regenerative medicine and tissue engineering strategies show great promise for treatment. These aim to restore tissue functions by combining principles of cell biology and engineering, using biomaterial scaffolds which can help in recapitulating the 3D environment of the brain and improving cell survival after grafting. Stroke and TBI are severe forms of disruptions of brain architecture, and two of the leading causes of mortality and morbidity worldwide, as no effective treatments are available. Several studies report how neural stem cells (NSCs) are able to improve functional recovery upon transplantation. However, the efficacy of these treatments is limited because of the mortality these cells are subject to after transplantation. In this context, the transplantation of mesenchymal cells (MSCs) has shown beneficial effects by secreting molecules and factors that help in the healing process. In this study, we tested alginate-based hydrogels as candidates to support human NSCs and MSCs transplantation into the brain, in the view of exploiting the beneficial effects of both and analyzing whether their combined use could have a synergistic effect. In the first part, we studied the suitability of alginate-based scaffolds for the three-dimensional encapsulation and culture of hNSCs and hMSCs. We analyzed their ability to support cell survival, and we evaluated whether changes in their concentration or modifications with ECM molecules could influence cell viability. We showed that the best survival conditions are found when using an RGDs-functionalized alginate scaffold at a low concentration (0.5% w/v). We then worked on the identification of the best conditions for MSCs culture and the definition of coculture conditions. Since serum is necessary for MSCs, but it is reported to induce glial differentiation of NSCs, we explored two different experimental setups. On one hand, we investigated the feasibility to exploit biomaterials to create "compartmentalized" cocultures that would at least partially retain serum. In parallel, we positively observed that MSCs can survive, proliferate and maintain their stemness even in absence of serum, supporting the hypothesis that the use of “compartmentalized” coculture systems would likely be exploitable for MSCs culture. Finally, we tested the reported beneficial effects of MSCs in our 3D culture system, in which NSCs do not show a great viability. Encapsulated NSCs were cultured on an MSCs monolayer, and we analyzed cell survival, proliferation, differentiation and stemness retention. Gene expression analyses highlighted that NSCs maintain stemness characteristics, but we were not able to observe any improvement in NSCs survival in coculture, with respect to standard culture. In the last part of the project we decided to test our system for tissue engineering approaches, exploiting axotomized brain organotypic slices (OSCs). We evaluated the presence of cells 7 days after transplantation, their integration in the OSCs and glial response. Preliminary results suggest that the biomaterial does not cause activation of glial cells, although stem cells do not seem to migrate out of scaffold and integrate into the brain slice.
APA, Harvard, Vancouver, ISO, and other styles
5

Speccher, Alessandra. "Tissue engineering approaches for brain injury applications." Doctoral thesis, Università degli studi di Trento, 2020. http://hdl.handle.net/11572/262798.

Full text
Abstract:
Due to the limited regenerative capacity of the central nervous system (CNS) upon injury, regenerative medicine and tissue engineering strategies show great promise for treatment. These aim to restore tissue functions by combining principles of cell biology and engineering, using biomaterial scaffolds which can help in recapitulating the 3D environment of the brain and improving cell survival after grafting. Stroke and TBI are severe forms of disruptions of brain architecture, and two of the leading causes of mortality and morbidity worldwide, as no effective treatments are available. Several studies report how neural stem cells (NSCs) are able to improve functional recovery upon transplantation. However, the efficacy of these treatments is limited because of the mortality these cells are subject to after transplantation. In this context, the transplantation of mesenchymal cells (MSCs) has shown beneficial effects by secreting molecules and factors that help in the healing process. In this study, we tested alginate-based hydrogels as candidates to support human NSCs and MSCs transplantation into the brain, in the view of exploiting the beneficial effects of both and analyzing whether their combined use could have a synergistic effect. In the first part, we studied the suitability of alginate-based scaffolds for the three-dimensional encapsulation and culture of hNSCs and hMSCs. We analyzed their ability to support cell survival, and we evaluated whether changes in their concentration or modifications with ECM molecules could influence cell viability. We showed that the best survival conditions are found when using an RGDs-functionalized alginate scaffold at a low concentration (0.5% w/v). We then worked on the identification of the best conditions for MSCs culture and the definition of coculture conditions. Since serum is necessary for MSCs, but it is reported to induce glial differentiation of NSCs, we explored two different experimental setups. On one hand, we investigated the feasibility to exploit biomaterials to create "compartmentalized" cocultures that would at least partially retain serum. In parallel, we positively observed that MSCs can survive, proliferate and maintain their stemness even in absence of serum, supporting the hypothesis that the use of “compartmentalized” coculture systems would likely be exploitable for MSCs culture. Finally, we tested the reported beneficial effects of MSCs in our 3D culture system, in which NSCs do not show a great viability. Encapsulated NSCs were cultured on an MSCs monolayer, and we analyzed cell survival, proliferation, differentiation and stemness retention. Gene expression analyses highlighted that NSCs maintain stemness characteristics, but we were not able to observe any improvement in NSCs survival in coculture, with respect to standard culture. In the last part of the project we decided to test our system for tissue engineering approaches, exploiting axotomized brain organotypic slices (OSCs). We evaluated the presence of cells 7 days after transplantation, their integration in the OSCs and glial response. Preliminary results suggest that the biomaterial does not cause activation of glial cells, although stem cells do not seem to migrate out of scaffold and integrate into the brain slice.
APA, Harvard, Vancouver, ISO, and other styles
6

Chong, Cassandra. "Improving 3D Scaffolds for Skin Tissue Engineering using Advanced Biotechnology." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/16551.

Full text
Abstract:
Existing, dermal, regenerative scaffolds facilitate dermal repair and wound healing of severe burn injuries; however, new tissue is often functionally, mechanically and aesthetically abnormal due to irregular deposition of new extracellular matrix. In the present study two novel, elastin-containing scaffolds were developed, characterised and examined both in vitro and in vivo aiming to minimise wound contraction, improve scar appearance and increase skin elasticity post-healing. The first types of scaffolds were electrospun from a triple polymer solution of collagen, elastin and poly(ϵ-caprolactone) (CEP). Two scaffolds were chosen for characterisation: CEP 1 was fabricated using a 1.5 % (w/v) collagen, 12 % (w/v) elastin and 1.5 % (w/v) poly(ϵ-caprolactone) (PCL) solution, a flow rate of 3 mL/h, an air gap of 15 cm and an applied electric potential of 25 kV; and CEP 2 was electrospun using a 2 % (w/v) collagen, 12 % (w/v) elastin and 1 % (w/v) PCL solution at 1 mL/h, 20 cm and 20 kV. In vitro cell studies using human, dermal fibroblasts (HDFs) and immortalised, human keratinocytes (HaCaTs) revealed CEP 1 and CEP 2 supported cell-seeding and cell proliferation with significantly higher proliferation of both cell types on CEP 1. Additionally, subcutaneous implant studies in mice revealed minimal inflammation in response to both scaffolds with CEP 1 vascularised by week 2 post-surgery. However, CEP 1 was rapidly biodegraded after 2 weeks. Collagen deposition was observed in encapsulating tissue and new tissue with consistent collagen expression over 24 weeks. The second type of scaffold investigated was an elastin-modified version of the commercial, dermal substitute Integra Dermal Regeneration Template (IDRT). Elastin-IDRT (EDRT) was developed by inclusion of 10% human tropoelastin and then investigated in comparison with IDRT. Morphological analysis by scanning electron microscope and mechanical characterisation revealed EDRT had significantly enlarged pores, higher porosity and increased deformability. Higher cell seeding efficiency of HaCaTs on EDRT was observed compared to IDRT but cell proliferation rate was found to be similar over 28 days. HDFs displayed increased cell growth rate on EDRT over 28 days compared to IDRT. Enhanced and accelerated HDF infiltration of EDRT was also visualised with complete infiltration by day 14 post-seeding. An in vivo, mouse, subcutaneous implant model showed that EDRT induced minimal inflammation. Gene expression of mouse collagen was consistent over 24 weeks with non-significant increases in elastin expression from weeks 2 and 4. One-step grafting demonstrated similar contraction between EDRT-, IDRT- and autografted wounds with final contraction around 40 % compared to 100 % in open wounds. EDRT displayed significantly accelerated, early-stage angiogenesis with higher vascularisation than IDRT-grafted, autografted or open wounds 2 weeks post grafting. By week 4 EDRT- and IDRT-grafted wounds had similar levels of vascularisation which were higher than autografted and open wounds. EDRT showed improved mechanical performance, supported enhanced cell interactions in vitro and accelerated angiogenesis in vivo. In summary, investigated scaffolds demonstrated properties that could potentially improve burn wound healing. The inclusion of elastin in scaffolds produced by either electrospinning or lyophilisation improved HDF infiltration and supported formation of a confluent layer of HaCaTs which could result in increased pliability of new skin and accelerated wound healing. In EDRT elastin improved scaffold porosity, pore size and accelerated angiogenesis in vivo indicating EDRT can facilitate and improve wound remodelling. Further investigation of both scaffolds is warranted especially due to the vascular inductive effects of EDRT and the synchronous spatial and temporal biodegradation of CEP 2 observed in vivo.
APA, Harvard, Vancouver, ISO, and other styles
7

Sharma, Aman. "Regenerative and biomimetic strategies in spinal surgery." Thesis, University of Oxford, 2015. http://ora.ox.ac.uk/objects/uuid:5eb692c3-1cad-43ff-aeb4-ff74382ee976.

Full text
Abstract:
Degenerative conditions of the spine are a major public health problem, leading to severe back pain, reduced quality of life and chronic disablement in a proportion of sufferers. For some of these patients, spinal fusion surgery is a treatment that can alleviate back pain and restore normal function. However, limitations in the availability of graft material mean that alternative grafts are needed and tissue-engineering approaches have been employed. Using a novel self-organising collagen scaffold combined with nano-hydroxyapatite and chondroitin sulphate and by employing the latest materials techniques, I have studied the osteogenic capability of a biomimetic graft for use in spinal fusion surgery. The mineralised collagen scaffold has compressive strength comparable to human cancellous bone and can support the proliferation of viable human mesenchymal stem cells. This porous scaffold can be combined with human mesenchymal stem cells to further promote bone growth, as evidenced by an upregulation in the levels of bone-forming genes and mineralisation of the scaffold. This scaffold can act as a carrier system for BMP-2, with wider application for other growth factors or drugs, providing sustained release when fabricated as a layer-by-layer scaffold. An alternative bone substitute for use in spinal surgery has been designed and characterised, with exciting potential for use in vivo.
APA, Harvard, Vancouver, ISO, and other styles
8

MUSCOLINO, Emanuela. "Polysaccharide hydrogels for regenerative medicine applications." Doctoral thesis, Università degli Studi di Palermo, 2022. http://hdl.handle.net/10447/535885.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Prajaneh, Saengsome. "Effect of cellular positional identity on bone regenerative capacity for tissue engineering." Thesis, King's College London (University of London), 2013. https://kclpure.kcl.ac.uk/portal/en/theses/effect-of-cellular-positional-identity-on-bone-regenerative-capacity-for-tissue-engineering(270579b0-278b-4a3d-9f4a-721f4d38e76e).html.

Full text
Abstract:
The aim of this study was to investigate the stability of positional identity markers and phenotypic differences in isolated osteoblasts from distinct anatomic regions. In addition, the ability of heterotypic co-cultures to reprogramme site-specific Hoxa gene expression also tested. Rat osteoblastic cells from femurs and calvariae were harvested as matched pairs of cultures from 4 male rats. Cells were expanded extensively in medium supplemented with FGF-2, and were shown to maintain their osteoblastic phenotype as characterised by alkaline phosphatase (ALP) staining, osteopontin (OPN), osteocalcin (OCN) expression and osteoblast-associated gene expression in long term culture. Gene expression of cells was determined by quantitative RT-PCR. Differences in Hoxa gene expression as markers of positional identity were maintained for up to at least 10 passages, with calvarial cells remaining Hoxa-ve throughout. The transcription factors Msx2 and Irx5 were consistently more highly expressed in calvarial cells, whereas Tbx3 expression was elevated in femoral cells. Expression of the osteoblast-associated genes Bglap and Sppl were elevated in femoral cells, and also associated with increased osteopontin secretion and bone nodule formation. Runx2 was elevated in calvarial cells. Cells were also pre-labelled with fluorescent vital staining and co-cultured for 7 days prior to separating by fluorescence activated cell sorter to investigate the possibility of re-programming of Hoxa negative cells by direct contact with Hoxa +ve cells. However no evidence was seen of modulation of positional identity genes and phenotypes in these heterotypic cultures. In conclusion, the results demonstrate persistence of expression of positional identity gene markers and phenotypic differences between femoral and calvarial osteoblasts for prolonged periods in culture. These data suggest that these differences in regionally defined osteoblasts are inherently programmed in the cells as a result of their embryological position. The results may have considerable implications when considering the transplantation of autologous cells in tissue engineering.
APA, Harvard, Vancouver, ISO, and other styles
10

Ueda, Yuichiro. "Application of Tissue Engineering with Xenogenic Cells and Tissues for Regenerative Medicine." 京都大学 (Kyoto University), 2004. http://hdl.handle.net/2433/147657.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "Regenerative engineering"

1

Khademhosseini, Ali. 3D Bioprinting in Regenerative Engineering. Boca Raton : Taylor & Francis, 2018.: CRC Press, 2018. http://dx.doi.org/10.1201/b21916.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Bernstein, Harold S., ed. Tissue Engineering in Regenerative Medicine. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-322-6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Pham, Phuc Van, ed. Tissue Engineering and Regenerative Medicine. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-19857-2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Bhatia, Sujata K., ed. Engineering Biomaterials for Regenerative Medicine. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4614-1080-5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Nair, Lakshmi S. Injectable hydrogels for regenerative engineering. Hackensack, NJ: Imperial College Press, 2016.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

Tissue engineering in regenerative medicine. New York: Humana Press, 2011.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

Cryogenic regenerative heat exchangers. New York: Plenum Press, 1997.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

1958-, Atala Anthony, ed. Principles of regenerative medicine. Amsterdam: Academic Press, 2008.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
9

NATO Advanced Research Workshop on Nanoengineered Systems for Regenerative Medicine (2007 Varna, Bulgaria). Advances in regenerative medicine: Role of nanotechnology and engineering principles. Dordrecht: Springer, 2010.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

Bhaskar, Birru, Parcha Sreenivasa Rao, Naresh Kasoju, Vasagiri Nagarjuna, and Rama Raju Baadhe, eds. Biomaterials in Tissue Engineering and Regenerative Medicine. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-0002-9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "Regenerative engineering"

1

Marei, Mona, Mohamad Nageeb, Rania M. Elbackly, Manal M. Saad, Ahmad Rashad, and Samer H. Zaky. "Tissue Engineering Alveolar Bone." In Regenerative Dentistry, 19–81. Cham: Springer International Publishing, 2010. http://dx.doi.org/10.1007/978-3-031-02581-5_2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Ripamonti, Ugo, Jean-Claude Petit, and June Teare. "Tissue Engineering of the Periodontal Tissues." In Regenerative Dentistry, 83–109. Cham: Springer International Publishing, 2010. http://dx.doi.org/10.1007/978-3-031-02581-5_3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Hansda, Anita, Sayan Mukherjee, Krishna Dixit, Santanu Dhara, and Gayatri Mukherjee. "Immunological Perspectives Involved in Tissue Engineering." In Regenerative Medicine, 37–55. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-6008-6_3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Dadhich, Prabhash, Parveen Kumar, Anirban Roy, and Khalil N. Bitar. "Advances in 3D Printing Technology for Tissue Engineering." In Regenerative Medicine, 181–206. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-6008-6_9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Tziafas, Dimitrios. "Dynamics for Pulp-Dentin Tissue Engineering in Operative Dentistry." In Regenerative Dentistry, 111–58. Cham: Springer International Publishing, 2010. http://dx.doi.org/10.1007/978-3-031-02581-5_4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Helsen, Jozef A., and Yannis Missirlis. "Tissue Engineering: Regenerative Medicine." In Biological and Medical Physics, Biomedical Engineering, 269–89. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-12532-4_13.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Hill, Michael J., Morteza Mahmoudi, and Parisa P. S. S. Abadi. "Nanobiomaterial Advances in Cardiovascular Tissue Engineering." In Cardiovascular Regenerative Medicine, 79–106. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-20047-3_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Reichert, Johannes C., A. Berner, Siamak Saifzadeh, and Dietmar W. Hutmacher. "Preclinical Animal Models for Segmental Bone Defect Research and Tissue Engineering." In Regenerative Medicine, 845–81. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9075-1_36.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Reichert, Johannes C., Arne Berner, Siamak Saifzadeh, and Dietmar W. Hutmacher. "Preclinical Animal Models for Segmental Bone Defect Research and Tissue Engineering." In Regenerative Medicine, 1023–64. Dordrecht: Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-5690-8_40.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Guzman, Gildardo, Muhammad Rafaqut, Sungreol Park, and Paul Y. Choi. "Regenerative Neural Electrodes." In Neural Interface Engineering, 281–98. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-41854-0_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Regenerative engineering"

1

Okada, Yohji, and Hideyuki Harada. "Active and Regenerative Control of Electrodynamic Vibration Damper." In ASME 1995 Design Engineering Technical Conferences collocated with the ASME 1995 15th International Computers in Engineering Conference and the ASME 1995 9th Annual Engineering Database Symposium. American Society of Mechanical Engineers, 1995. http://dx.doi.org/10.1115/detc1995-0621.

Full text
Abstract:
Abstract A new method of regenerating electric power from the vibration energy is presented. An electro-dynamic actuator is controlled by a relay controller to regenerate electric power during the highspeed motion of the actuator. For the lowspeed motion, an active control algorithm is applied to the same actuator to achieve good damping performance. This idea can be applied to a vehicle suspension system which is composed of electrodynamic actuator and spring. The system is simulated on a computer to evaluate the vibration damping property and energy regenerative capability. A simple experimental apparatus is made to confirm its capability.
APA, Harvard, Vancouver, ISO, and other styles
2

Si, Junping, Mingyan Tong, Wenhua Yang, and Gang Huang. "Study on Thermal Characteristics of the Regenerative Heat Exchanger." In 2016 24th International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/icone24-60380.

Full text
Abstract:
The regenerative heat exchanger is widely used in nuclear power plants and research reactors. It is composed of the regeneration section and the cooling section. The heat transfer mainly occurs at the cooling section, while the regeneration section is designed to reduce the temperature difference between the hot and cold fluids and weaken the damage to the heat exchanger due to the existence of thermal stress. Meanwhile, some heat is also can be recovered through the regeneration section. This paper mainly aims to analyze the thermal characteristics of the regenerative heat exchanger according to its structure properties, and provides some suggestions for regenerative heat exchanger design based on the influence of some key factors on thermal characteristics. The results show that improving the outlet temperature in the regeneration section primary side can both reduce the heat exchange areas of the regeneration section and the cooling section, but this will rise thermal shock and increase the operation safety risk. The baffles arrangement will enhance heat exchange capacity, and the heat exchange area decreases with the baffle gap height increasing. With the heat exchange area margin of the regeneration section improvement, the actual power will gradually reduce. The measures, including increasing secondary water flow or taking a corresponding margin about 52.8%∼59.2% that of the regeneration section for the cooling section heat exchange area, can be taken to overcome the adverse effects of the margin on the regenerative heat exchanger. More heat exchange areas of the regeneration section and the cooling section are required to satisfy the rated power with the fouling thermal resistance of the primary water increasing. Moreover, adopting a lower fouling coefficient favors the generative heat exchanger running under the design power.
APA, Harvard, Vancouver, ISO, and other styles
3

Kim, Sang-Soo, and Yohji Okada. "Energy Regenerative Damper Using Pulse Width Modulated (PWM) Step-up Chopper." In ASME 1999 Design Engineering Technical Conferences. American Society of Mechanical Engineers, 1999. http://dx.doi.org/10.1115/detc99/movic-8415.

Full text
Abstract:
Abstract This paper describes a new technique of improving the efficiency of an energy regenerative damper. It is intended for an electro-dynamic type vibration damper to regenerate vibration energy efficiently. Normally regenerative damper can regenerate vibration energy only at high speed motion. For low speed motion, the damper has nonlinear characteristics with dead zone and the energy is not regenerated. In order to overcome the problem of dead zone, a step-up chopper is introduced between the actuator and the charging circuit. The energy is regenerated from the low speed and low voltage actuator to high voltage charging circuit. This paper also proposes a new control technique to the step-up chopper by using pulse width modulated (PWM) signal. The proposed damper is applied to a dynamic damper system and is simulated on a computer. A simple experiment is performed to confirm the proposed technique. The results show an improvement in performance than the usual regenerative damper.
APA, Harvard, Vancouver, ISO, and other styles
4

Zou, Zhongyue, Junyi Cao, Chengbin Ma, and Huarong Zhang. "A Measurement System for Electric Vehicle Powered by Supercapacitors." In ASME 2011 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/detc2011-47138.

Full text
Abstract:
Due to the high power density of supercapacitors, it is adopted in electric vehicles to essentially guarantee to recover more regenerative energy. However, there is still not a valid set of measurement methods to gauge the energy absorbed from regeneration system, the measurement and evaluation system for regenerative energy require to be investigated in order to estimate the performance of electric vehicles. Based on the analysis of the regenerative braking energy system of a supercapacitor truck, a measurement and evaluation system for recycling energy in the braking process is established. Meanwhile, the experiments of supercapacitor vehicle under various braking condition are carried out. The results show the effectiveness of the proposed braking energy recycling measurement method.
APA, Harvard, Vancouver, ISO, and other styles
5

Sinha, Ashish, Yogendra Joshi, and Bruce H. Storm. "Thermoelectric Cooler Based Regenerative Adsorption Refrigeration System for High Temperature Electronics." In ASME 2007 InterPACK Conference collocated with the ASME/JSME 2007 Thermal Engineering Heat Transfer Summer Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/ipack2007-33235.

Full text
Abstract:
This paper presents the analytical study of a thermoelectric cooler (TEC) based two-stage regenerative adsorption cycle with evaporator temperature ranging from 80°C to 180°C and heat rejection temperature ranging from 200°C to 230°C. This proposed cycle restricts the highest temperature at which the TEC regenerates heat by reducing the maximum bed temperature during the desorption phase, which leads to the possibility of the system being realized with commercially available TECs and a reasonable TEC coefficient of performance (COP∼ 0.4). The low COP (<1) of the TEC results in excess heat at the desorption bed during heat regeneration. If the excess heat is rejected to the environment, COP gains arising from regeneration are reduced. Using the TEC to regenerate only part of the heat helps to mitigate this problem. The adsorption cycle in conjunction with TEC can pump heat through larger temperature differences with system efficiency much greater than that of a TEC used alone under identical conditions. The study aims to extend the limits of the adsorption refrigeration systems to provide compact cooling devices for harsh environments.
APA, Harvard, Vancouver, ISO, and other styles
6

Swette, Larry L., Nancy D. Kackley, and Anthony B. LaConti. "Regenerative Fuel Cells." In 27th Intersociety Energy Conversion Engineering Conference (1992). 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 1992. http://dx.doi.org/10.4271/929087.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Zhao, Huyue, and Kornel F. Ehmann. "Regenerative Chatter in High-Speed Tandem Rolling Mills." In ASME 2006 International Manufacturing Science and Engineering Conference. ASMEDC, 2006. http://dx.doi.org/10.1115/msec2006-21014.

Full text
Abstract:
Third-octave-mode chatter, the most detrimental form of rolling chatter, is generated by means of negative damping, mode coupling, and regeneration. While mechanisms that include negative damping, and mode coupling have been thoroughly investigated, those associated with the regenerative effect remain elusive. In this paper, the mechanisms that may lead to regenerative chatter are studied through a state-space representation of a multi-stand mill that is constructed by coupling a homogenous dynamic rolling process model with a structural model for the mill stands in a high-speed tandem mill configuration. Stability analysis, by using the integral criterion for the stability of systems described by delay differential equations, is carried out for the regenerative mechanism in order to better understand the effects of rolling parameters on a single stand as well as the overall system. Preliminary simulation results, based on the proposed chatter model, are presented to demonstrate the feasibility and the accuracy of the chatter model, as well as to investigate chatter phenomena too complex to be studied analytically.
APA, Harvard, Vancouver, ISO, and other styles
8

Golchert, B., S. L. Chang, C. Q. Zhou, and J. Wang. "Modeling of Regenerative Furnace Ports." In ASME 2003 International Mechanical Engineering Congress and Exposition. ASMEDC, 2003. http://dx.doi.org/10.1115/imece2003-42321.

Full text
Abstract:
In order to increase overall efficiency, many industrial glass furnaces are regenerative; that is, the heat from the exhaust gases is used to preheat in the in-coming combustion air. The ports on these furnaces inject stream(s) of fuel into the preheated air stream and then combustion occurs inside the combustion chamber. Modeling of the exact detail of these furnace ports in addition to modeling the combustion space proper becomes computationally burdensome since many of these furnaces are extremely large. This paper presents an engineering approach using computational fluid dynamics to model both the major effects of the furnace ports in addition to calculating the detailed flow field in the combustion space. This approximation has been incorporated into a complete (combustion space/glass melt) furnace simulation. This engineering approach significantly reduces run time while still maintaining results that represent the conditions seen in the furnace. This paper will present this approach as well as some preliminary comparisons with actual furnace data/observations.
APA, Harvard, Vancouver, ISO, and other styles
9

Insperger, Ta´mas, Ga´bor Ste´pa´n, Ferenc Hartung, and Janos Turi. "State Dependent Regenerative Delay in Milling Processes." In ASME 2005 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2005. http://dx.doi.org/10.1115/detc2005-85282.

Full text
Abstract:
Traditional models of regenerative machine tool chatter use constant time delays assuming that the period between two subsequent cuts is a constant determined definitely by the spindle speed. These models result in delay-differential equations with constant time delay. If the vibrations of the tool relative to the workpiece are also included in the surface regeneration model, then the resulted time delay is not constant, but it depends on the actual and a delayed position of the tool. In this case, the governing equation is a delay-differential equation with state dependent time delay. Equations with state dependent delays can not be linearized in the traditional sense, but there exists linear equations that can be associated to them. This way, the local behavior of the system with state dependent delays can be investigated. In this study, a two degree of freedom model is presented for milling process. A thorough modeling of the regeneration effect results in the governing delay-differential equation with state dependent time delay. It is shown through the linearization of the nonlinear equation that an additional term arises in the linearized equation of motion due to the state-dependency of the time delay.
APA, Harvard, Vancouver, ISO, and other styles
10

Wyczalek, Floyd A., and Tsih C. Wang. "Electric Vehicle Regenerative Braking." In 27th Intersociety Energy Conversion Engineering Conference (1992). 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 1992. http://dx.doi.org/10.4271/929139.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "Regenerative engineering"

1

Research, Gratis. Regenerative Medicine: A Breakthrough in the Branch of Medicine. Gratis Research, November 2020. http://dx.doi.org/10.47496/gr.blog.04.

Full text
Abstract:
Regenerative medicine, being an interdisciplinary field, applies the principle of engineering and life science to promote regeneration. Regenerative medicine supports the treatment of chronic diseases and acute insults
APA, Harvard, Vancouver, ISO, and other styles
2

Michael A. Inbody, Rodney L. Borup, James C. Hedstrom, Jose Tafoya, Byron Morton, Lois Zook, and Nicholas E. Vanderborgh. Regenerative fuel cell engineering - FY99. Office of Scientific and Technical Information (OSTI), January 2000. http://dx.doi.org/10.2172/752398.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Drummond, Colin K., Mark S. Rice, and Paul Bode. Engineering Design Study for the Adaptation, Assembly, and Installation of a Regenerative Mechanical Differential Steer Unit for Tracked Amphibious Vehicle. Fort Belvoir, VA: Defense Technical Information Center, February 1987. http://dx.doi.org/10.21236/ada204910.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Handa, Avtar K., Yuval Eshdat, Avichai Perl, Bruce A. Watkins, Doron Holland, and David Levy. Enhancing Quality Attributes of Potato and Tomato by Modifying and Controlling their Oxidative Stress Outcome. United States Department of Agriculture, May 2004. http://dx.doi.org/10.32747/2004.7586532.bard.

Full text
Abstract:
General The final goal and overall objective of the current research has been to modify lipid hydroperoxidation in order to create desirable phenotypes in two important crops, potato and tomato, which normally are exposed to abiotic stress associated with such oxidation. The specific original objectives were: (i) the roles of lipoxygenase (LOX) and phospholipids hydroperoxide glutathione peroxidase (PHGPx) in regulating endogenous levels of lipid peroxidation in plant tissues; (ii) the effect of modified lipid peroxidation on fruit ripening, tuber quality, crop productivity and abiotic stress tolerance; (iii) the effect of simultaneous reduction of LOX and increase of PHGPx activities on fruit ripening and tuber quality; and (iv) the role of lipid peroxidation on expression of specific genes. We proposed to accomplish the research goal by genetic engineering of the metabolic activities of LOX and PHGPx using regulatable and tissue specific promoters, and study of the relationships between these two consecutive enzymes in the metabolism and catabolism of phospholipids hydroperoxides. USA Significant progress was made in accomplishing all objectives of proposed research. Due to inability to regenerate tomato plants after transforming with 35S-PHGPx chimeric gene construct, the role of low catalase induced oxidative stress instead of PHGPx was evaluated on agronomical performance of tomato plant and fruit quality attributes. Effects of polyamine, that protects DNA from oxidative stress, were also evaluated. The transgenic plants under expressing lipoxygenase (LOX-sup) were crossed with catalase antisense (CAT-anti) plants or polyamine over producing plants (SAM-over) and the lines homozygous for the two transgenes were selected. Agronomical performance of these line showed that low catalase induced oxidative stress negatively affected growth and development of tomato plants and resulted in a massive change in fruit gene expression. These effects of low catalase activity induced oxidative stress, including the massive shift in gene expression, were greatly overcome by the low lipoxygenase activity. Collectively results show that oxidative stress plays significant role in plant growth including the fruit growth. These results also for the first time indicated that a crosstalk between oxidative stress and lipoxygenase regulated processes determine the outcome during plant growth and development. Israel Regarding PHGPx, most of the study has concentrated on the first and the last specific objectives, since it became evident that plant transformation with this gene is not obvious. Following inability to achieve efficient transformation of potato and tomato using a variety of promoters, model plant systems (tobacco and potato cell cultures, tobacco calli and plantlets, and Arabidopsis) were used to establish the factors and to study the obstacles which prohibited the regeneration of plants carrying the genetic machinery for overproduction of PHGPx. Our results clearly demonstrate that while genetic transformation and over-expression of PHGPx occurs in pre-developmental tissue stage (cell culture, calli clusters) or in completed plant (Arabidopsis), it is likely that over-expression of this enzyme before tissue differentiation is leading to a halt of the regeneration process. To support this assumption, experiments, in which genetic engineering of a point-mutated PHGPx gene enable transformation and over-expression in plants of PhSPY modified in its catalytic site and thus inactive enzymatically, were successfully carried out. These combined results strongly suggest, that if in fact, like in animals and as we established in vitro, the plant PHGPx exhibits PH peroxidase activity, these peroxides are vital for the organisms developmental process.
APA, Harvard, Vancouver, ISO, and other styles
5

Perl, Avichai, Bruce I. Reisch, and Ofra Lotan. Transgenic Endochitinase Producing Grapevine for the Improvement of Resistance to Powdery Mildew (Uncinula necator). United States Department of Agriculture, January 1994. http://dx.doi.org/10.32747/1994.7568766.bard.

Full text
Abstract:
The original objectives are listed below: 1. Design vectors for constitutive expression of endochitinase from Trichoderma harzianum strain P1. Design vectors with signal peptides to target gene expression. 2. Extend transformation/regeneration technology to other cultivars of importance in the U.S. and Israel. 3. Transform cultivars with the endochitinase constructs developed as part of objective 1. A. Characterize foliar powdery mildew resistance in transgenic plants. Background of the topic Conventional breeding of grapevines is a slow and imprecise process. The long generation cycle, large space requirements and poor understanding of grapevine genetics prevent rapid progress. There remains great need to improve existing important cultivars without the loss of identity that follows from hybridization. Powdery mildew (Uncinula necator) is the most important fungal pathogen of grapevines, causing economic losses around the world. Genetic control of powdery mildew would reduce the requirement for chemical or cultural control of the disease. Yet, since the trait is under polygenic control, it is difficult to manipulate through hybridization and breeding. Also, because grapevines are heterozygous and vegetatively propagated cultivar identity is lost in the breeding process. Therefore, there is great need for techniques to produce transgenic versions of established cultivars with heterologous genes conferring disease resistance. Such a gene is now available for control of powdery mildew of grapevines. The protein coded by the Endochitinase gene, derived from Trichoderma harzianum, is very effective in suppressing U. necator growth. The goal of this proposal is to develop transgenic grapevines with this antifungal gene, and to test the effect of this gene on resistance to powdery mildew. Conclusions, achievements and implications Gene transfer technology for grape was developed using commercial cultivars for both wine and table grapes. It paved the way for a new tool in grapevine genetic studies enabling the alteration of specific important traits while maintaining the essential features of existing elite cultivars. Regeneration and transformation technologies were developed and are currently at an advanced stage for USA wine and Israeli seedless cultivars, representing the cutting edge of grape genetic engineering studies worldwide. Transgenic plants produced are tested for powdery mildew resistance in greenhouse and field experiments at both locations. It is our ultimate goal to develop transgenic grapes which will be more efficient and economical for growers to produce, while also providing consumers with familiar products grown with reduced chemical inputs.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Regenerative engineering' for particular source types:
Journal articles Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography