Relevant bibliographies by topics / Recombinase mediated cassette exchange

Academic literature on the topic 'Recombinase mediated cassette exchange'

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Published: 11 March 2023

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Journal articles on the topic "Recombinase mediated cassette exchange"

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Soares, Hugo R., Ana I. Almeida, Hélio A. Tomás, Paula M. Alves, and Ana S. Coroadinha. "Flexible pseudotyping of retrovirus using recombinase-mediated cassette exchange." Biotechnology Letters 40, no. 4 (January 20, 2018): 633–39. http://dx.doi.org/10.1007/s10529-018-2515-6.

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Nakano, M. "Production of viral vectors using recombinase-mediated cassette exchange." Nucleic Acids Research 33, no. 8 (April 28, 2005): e76-e76. http://dx.doi.org/10.1093/nar/gni074.

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Premsrirut, Prem K., Lukas E. Dow, Youngkyu Park, Gregory J. Hannon, and Scott W. Lowe. "Creating Transgenic shRNA Mice by Recombinase-Mediated Cassette Exchange." Cold Spring Harbor Protocols 2013, no. 9 (September 2013): pdb.prot077057. http://dx.doi.org/10.1101/pdb.prot077057.

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Louwerse, Jeanine D., Miranda C. M. van Lier, Dirk M. van der Steen, Clementine M. T. de Vlaam, Paul J. J. Hooykaas, and Annette C. Vergunst. "Stable Recombinase-Mediated Cassette Exchange in Arabidopsis Using Agrobacterium tumefaciens." Plant Physiology 145, no. 4 (October 5, 2007): 1282–93. http://dx.doi.org/10.1104/pp.107.108092.

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Murray, Johanne M., Adam T. Watson, and Antony M. Carr. "Identifying Products of Recombinase-Mediated Cassette Exchange (RMCE) inSchizosaccharomyces pombe." Cold Spring Harbor Protocols 2016, no. 5 (May 2016): pdb.prot090944. http://dx.doi.org/10.1101/pdb.prot090944.

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Turan, Soeren, Melanie Galla, Ellen Ernst, Junhua Qiao, Christine Voelkel, Bernhard Schiedlmeier, Christoph Zehe, and Juergen Bode. "Recombinase-Mediated Cassette Exchange (RMCE): Traditional Concepts and Current Challenges." Journal of Molecular Biology 407, no. 2 (March 2011): 193–221. http://dx.doi.org/10.1016/j.jmb.2011.01.004.

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Nonet, Michael L. "Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange." Genetics 215, no. 4 (June 8, 2020): 903–21. http://dx.doi.org/10.1534/genetics.120.303388.

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The application of CRISPR technology has greatly facilitated the creation of transgenic Caenorhabditis elegans lines. However, methods to insert multi-kilobase DNA constructs remain laborious even with these advances. Here, I describe a new approach for introducing large DNA constructs into the C. elegans genome at specific sites using a combination of Flp and Cre recombinases. The system utilizes specialized integrated landing sites that express GFP ubiquitously flanked by single loxP, FRT, and FRT3 sites. DNA sequences of interest are inserted into an integration vector that contains a sqt-1 self-excising cassette and FRT and FRT3 sites. Plasmid DNA is injected into the germline of landing site animals. Transgenic animals are identified as Rol progeny, and the sqt-1 marker is subsequently excised with heat shock Cre expression. Integration events were obtained at a rate of approximately one integration per three injected F0 animals—a rate substantially higher than any current approach. To demonstrate the robustness of the approach, I compared the efficiency of the Gal4/UAS, QF (and QF2)/QUAS, tetR(and rtetR)/tetO, and LexA/lexO bipartite expression systems by assessing expression levels in combinations of driver and reporter GFP constructs and a direct promoter GFP fusion each integrated at multiple sites in the genome. My data demonstrate that all four bipartite systems are functional in C. elegans. Although the new integration system has several limitations, it greatly reduces the effort required to create single-copy insertions at defined sites in the C. elegans genome.
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Sorrell, David A., Claire J. Robinson, Jo-Ann Smith, and Andreas F. Kolb. "Recombinase mediated cassette exchange into genomic targets using an adenovirus vector." Nucleic Acids Research 38, no. 11 (April 5, 2010): e123-e123. http://dx.doi.org/10.1093/nar/gkq192.

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Sun, Florence F., Justine E. Johnson, Martin P. Zeidler, and Jack R. Bateman. "Simplified Insertion of Transgenes Onto Balancer Chromosomes via Recombinase-Mediated Cassette Exchange." G3: Genes|Genomes|Genetics 2, no. 5 (May 2012): 551–53. http://dx.doi.org/10.1534/g3.112.002097.

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Cesari, Francesca, Verena Rennekampff, Kristina Vintersten, Lam Giang Vuong, Jost Seibler, J�rgen Bode, Franziska F. Wiebel, and Alfred Nordheim. "Elk-1 knock-out mice engineered by Flp recombinase-mediated cassette exchange." genesis 38, no. 2 (2004): 87–92. http://dx.doi.org/10.1002/gene.20003.

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Dissertations / Theses on the topic "Recombinase mediated cassette exchange"

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Penfold, Catherine. "The development of a large interval recombinase mediated cassette exchange (RMCE) strategy." Thesis, University of Edinburgh, 2005. http://hdl.handle.net/1842/12256.

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Murine embryonic stem (ES) cells have provided researchers with a useful tool to investigate genome function and the consequences of genome mutation. One mutational approach is gene-targeting, this involves the introduction of DNA sequences of choice, precisely, to almost any location in the target genome by homologous recombination. At present, most gene-targeting strategies introduce DNA constructs that derive from plasmids. Plasmids can stably propagate up to approximately 30 kb of DNA. Therefore, this size limit may place a restriction on the range of mutations that may be made to a genome using a single plasmid-derived gene-targeting construct alone. To overcome this limitation, multiple rounds of sequential gene-targeting experiments may be performed, however such an approach may be too lengthy to be practicable. In order to address this current limitation with gene-targeting a novel strategy was tested, implementing Cre-lox site-specific recombination (SSR) technology and the bacterial artificial chromosome (BAC) vector system. Two sequential gene-targeting events in murine E14Tg2a ES cells (HPRT) were performed at separate locations to chromosome 11. The aim of gene-targeting was to create an interval on chromosome 11 that included a single copy of the murine alpha-globin locus, between the hetero-specific lox sites, loxP and lox511, an interval of approximately 64 kb. To this end the first targeting event delivered lox511 /hygromycin/I See Illox51 J sequences and the second event frt/I See I/5'hprt//oxP/neomycin sequences. ES cells that were confirmed to have correctly undergone the two desired targeting events (double-targeted) were then assessed to determine whether these events had occurred to the same chromosome 11 (in eis ), as desired, or to the alternate copies of chromosome 11 (in trans). This assessment involved restricting DNA from the double-targeted ES cell lines with the rare-cutting restriction endonuclease I See I and resolving the products of this restriction by pulsed field gel electrophoresis. This analysis identified two in cis lines (CAT-A3 and CAT-B3) and an in trans line (CATCIO). The double-targeted ES cell lines were then further characterised to determine whether the hetero-specific lox sites they harboured would participate in ere-mediated SSR. The positive result of this analysis was the generation of ES cell clones that were hemizygous for the alpha-globin locus, a deletion of 64 kb. Hemizygous ES cell clones were obtained from the CAT-A3 and CAT-B3 ES cell lines, as predicted, but not from the CAT-C 10 line, although all the lines tested showed evidence of SSR occurring. In parallel to achieving the interval between loxP and lox51 l in ES cells, a BAC, harbouring the alpha-globin locus, was similarly modified with lox sites using recombination-mediated cloning. The aim of the BAC modification was to create an interval between lox sites in the BAC identical to that achieved in the ES cells. The BAC was targeted sequentially with two separate constructs, lox511/k.anamycin/lox511/HSVtk and then blasticidin/loxP/3'hprt/I See 11.frt. The correct targeting of the BAC was verified by restricting its DNA with a panel restriction endonucleases. The lox sites were then tested in an in vitro analysis with purified Cre recombinase and found to be competent to participate in SSR reactions. The modified BAC was co-electroporated with a Cre expression plasmid into the CAT-A3 and CAT-B3 ES cell lines, previously characterised as targeted in eis, with the aim of exchanging the interval sequences in the ES cell with those of the BAC. The ultimate aim of such an exchange would be to deliver any combination of mutations that would be previously engineered to the BAC interval, to that of the ES cell, by a single SSR event. This experimental approach should expedite and facilitate the mutational analysis of gene loci. To generate comparative data the result of SSR between the modified BAC and an in trans targeted ES cell line (CAT-CI 0) was also assessed. The selection for the desired exchange involved reconstruction of an Hprt minigene and exclusion of a thymidine kinase gene, cells which haboured these events could therefore be selected for in HAT and ganciclovir supplemented media respectively. ES cell clones generated from both of the in cis lines tested (CAT-A3 and CAT-B3) had the correct selection resistance profiles, thus indicating that the desired exchange had been achieved in these clones. Additionally, Southern blot analysis from the DNA from these clones was consistent with the achievement of the desired exchange. However, the results obtained from clones generated from the in trans line (CAT-CI 0) were not consistent with their predicted genetic arrangement following SSR with the modified BAC. Thus far similar experimental approaches have been implemented to exchange smaller intervals of I to 5 kb and have been termed recombinase mediated cassette exchange (RMCE). However the experiments described within this thesis are the first test whether the same rationale may be applied to larger intervals. The strategy described and tested in this thesis has therefore been termed large interval RMCE (liRMCE).
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Messineo, Stefania. "Development of a gene targeting strategy (Recombinase-Mediated CAssette Exchange) to generate cellular models of MYH9-related disease." Doctoral thesis, Università degli studi di Trieste, 2011. http://hdl.handle.net/10077/4603.

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2009/2010
La malattia MYH9-correlata (MYH9-RD) è una malattia autosomica dominante, caratterizzata da trombocitopenia congenita con piastrine di grandi dimensioni, aggregati nei neutrofili, sordità progressiva, cataratta e nefropatia. La MYH9-RD è causata da mutazioni nel gene MYH9 che codifica per la catena pesante della miosina non muscolare di classe II (miosina-9). I meccanismi patogenetici che causano questa malattia non sono ancora stati chiaramente identificati e il loro studio è complicato dalla mancanza di adeguati modelli cellulari e animali. Lo scopo di questo progetto è stato di generare un modello in vitro per studiare la funzione della miosina-9 e il ruolo di due mutazioni che incorrono nel gene MYH9: la R702C e la R1933X, che correlano rispettivamente con un fenotipo grave e lieve. Per questo motivo abbiamo deciso di manipolare le cellule staminali embrionali murine (ES), che sono pluripotenti e possono essere differenziate in diversi linee cellulari, compresa la linea megacariocitica. Per ingegnerizzare queste cellule ad alta efficienza abbiamo messo a punto una strategia nota come "scambio di cassette mediato da ricombinasi" (RMCE). Dopo l'integrazione di una cassetta fiancheggiata da siti FRT (sequenze di riconoscimento per l'enzima flippasi), il sistema ci ha permesso di scambiare diverse sequenze di DNA in presenza dell'enzima flippasi. Quindi il primo esone codificante del gene Myh9 è stato distrutto dall'inserimento, mediante ricombinazione omologa, di una cassetta fiancheggiata da due siti FRT contenente il gene reporter Beta-galattosidasi. Successivamente abbiamo scambiato questa cassetta con altre tre contenenti il cDNA Myh9 murino wild-type e i due mutati, generando i cloni ES che esprimono queste sequenze esogene sotto il controllo del promotore Myh9 endogeno. La caratterizzazione a livello dell'RNA e delle proteine di questi cloni ci ha portato a stabilire che gli alleli mutati e wild-type sono espressi allo stesso livello, suggerendo che le manipolazioni genetiche non interferiscono con i corretti meccanismi fisiologici di trascrizione e traduzione del gene Myh9. Tuttavia, mediante Western Blot abbiamo mostrato che la proteina miosina-9 è espressa a livello inferiore nei cloni mutati rispetto ai wild-type. Le analisi di immunofluorescenza per indagare la presenza di aggregati di miosina-9, che sono sempre presenti nei neutrofili di pazienti, non hanno rilevato alcuna variazione nella distribuzione della miosina-9, fatta eccezione per un segnale di intensità minore. Questi risultati indicano che, nonostante l'espressione dell'allele ingegnerizzato sia normale, la proteina mutata sembra essere degradata, almeno nelle cellule ES murine, determinando un effetto di aploinsufficienza delle mutazioni R702C e R1933X. Per accertare la loro pluripotenza, abbiamo differenziato dei cloni ES in corpi embrioidi e cardiomiociti, senza rivelare alcuna differenza tra i cloni mutanti e i wild-type. Dal momento che una caratteristica congenita dei pazienti MYH9-RD è la macrotrombocitopenia, abbiamo sviluppato un protocollo per differenziare i cloni mutati ES in megacariociti per indagare come le mutazioni in MYH9 portino a una impropria produzione di piastrine. In conclusione, per studiare la MYH9-RD abbiamo sviluppato una strategia che ci ha permesso di esprimere sequenze di interesse in cellule ES di topo sotto il controllo del promotore Myh9 endogeno. La differenziazione in vitro di queste cellule ci permetterà di studiare l'effetto delle mutazioni nel corso della megacariocitopoiesi. Inoltre, poiché le cellule ES possono anche essere usate per generare modelli animali, questa strategia ci permetterà di testare diverse ipotesi patogenetiche in vitro, prima di passare a studi in vivo.
XXIII Ciclo
1982
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Turan, Sören [Verfasser], Jürgen [Akademischer Betreuer] Bode, and Beate [Akademischer Betreuer] Sodeik. "Versatile extensions of the Flp-recombinase-mediated cassette exchange technology : RMCE multiplexing approaches meet the needs for predictable genome engineering / Sören Turan. Institut Experimentelle Hämatologie der Medizinische Hochschule Hannover. Betreuer: Jürgen Bode ; Beate Sodeik." Hannover : Bibliothek der Medizinischen Hochschule Hannover, 2011. http://d-nb.info/1012969827/34.

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Schinkowski, Christian In-Shu Verfasser], and Ralf-Rainer [Akademischer Betreuer] [Mendel. "Establishing site-specific recombination mediated cassette exchange in Pichia pastoris and development of an optimized process for the production of mouse Tmprss2 / Christian In-Shu Schinkowski ; Betreuer: Ralf-Rainer Mendel." Braunschweig : Technische Universität Braunschweig, 2017. http://d-nb.info/1175817430/34.

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Fernandes, Fabiana Carreira. "Establishment and evaluation of flexible insect cell lines for rapid production of recombinant proteins." Doctoral thesis, 2015. http://hdl.handle.net/10362/15270.

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Book chapters on the topic "Recombinase mediated cassette exchange"

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Kolb, Andreas F., Christopher Knowles, Patrikas Pultinevicius, Jennifer A. Harbottle, Linda Petrie, Claire Robinson, and David A. Sorrell. "Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors." In Methods in Molecular Biology, 127–50. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7169-5_9.

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Voziyanova, Eugenia, Rachelle P. Anderson, and Yuri Voziyanov. "Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases." In Methods in Molecular Biology, 53–67. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7169-5_4.

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Ebinuma, Hiroyasu, Kazuya Nanto, Saori Kasahara, and Atsushi Komamine. "Marker-Free Gene Targeting by Recombinase-Mediated Cassette Exchange." In Methods in Molecular Biology, 379–90. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-558-9_30.

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Roebroek, Anton J. M., and Bart Van Gool. "Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE)." In Methods in Molecular Biology, 63–76. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1215-5_4.

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Vidigal, João, Fabiana Fernandes, Ana S. Coroadinha, Ana P. Teixeira, and Paula M. Alves. "Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology." In Animal Cell Biotechnology, 15–27. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-733-4_2.

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"RMCE (recombinase mediated cassette exchange)." In Encyclopedia of Genetics, Genomics, Proteomics and Informatics, 1720. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6754-9_14727.

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