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Dissertations / Theses on the topic 'Recombinant protein'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Koscky, Paier Carlos Roberto 1983. "Padronização da expressão heterologa e de modelo de ensaio de atividade para a proteina quinase humana S6K." [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314787.

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Orientador: Nilson Ivo Tonin Zanchin<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-08-14T12:40:52Z (GMT). No. of bitstreams: 1 KosckyPaier_CarlosRoberto_M.pdf: 3760581 bytes, checksum: 99331529324819b59a4360d60efd9b9a (MD5) Previous issue date: 2009<br>Resumo: A quinase de 70 kDa da proteína ribossomal S6, isoforma 1 (S6K1), é uma fosfoproteína implicada na regulação de genes relacionados ao controle da tradução em mamíferos e possui uma forma nuclear (a1) e uma citoplasmática (a2). A fosforilação do seu principal alv

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2

Tse, Muk-hei. "Investigations on recombinant Arabidopsis acyl-coenzyme A binding protein 1." View the Table of Contents & Abstract, 2005. http://sunzi.lib.hku.hk/hkuto/record/B36427664.

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3

Tse, Muk-hei, and 謝牧熙. "Investigations on recombinant Arabidopsis acyl-coenzyme A binding protein 1." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B36427664.

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4

Corgozinho, Carolina Nunes Costa. "Desenvolvimento de vacina baseada em sistema de liberação sustentada contendo proteína recombinante." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/60/60137/tde-31072009-083709/.

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No Brasil, e em outros paises de clima tropical, os carrapatos se tornaram um enorme problema economico de^$de que a industria do gado se desenvolveu. O carrapato Boophilus microplus, um dos artropodes mais importantes na veterinaria, causa efeitos direto, como suc,cao de sangue, e indireto, como a transmissao de uma grande variedade de patogenos que normalmente resulta em infec,c~es letais. As vacinas genicas contendo o antigeno Bm86, uma proteina ligada a membrana do intestino do carrapato B. microplus, representam uma alternativa atrativa aos acaricidas para controlar as infesta~oes por car

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Ndabambi, Nonkululeko. "Recombinant expression of the pRb- and p53-interacting domains from the human RBBP6 protein for in vitro binding studies." Thesis, University of the Western Cape, 2004. http://etd.uwc.ac.za/index.php?module=etd&amp.

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The aim of this thesis was to produce DNA expression constructs and use them to investigate the feasibility of recombinantly expression proteins for future interaction studies between human RBBP6 and p53 and pRb proteins.

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6

Barua, Bipasha. "Design and study of Trp-cage miniproteins /." Thesis, Connect to this title online; UW restricted, 2005. http://hdl.handle.net/1773/8533.

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7

Bleckwenn, Nicole Aleece. "Protein production development with recombinant vaccinia virus." College Park, Md. : University of Maryland, 2004. http://hdl.handle.net/1903/1416.

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Thesis (Ph. D.) -- University of Maryland, College Park, 2004.<br>Thesis research directed by: Chemical Engineering. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.

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Morreale, Giacomo. "Processing of recombinant fusion protein and peptide." Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.615060.

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9

Pesarrodona, Roches Mireia. "Supramolecular organisation and biological properties of tumor targeted, self-assembling protein nanoparticles." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/402365.

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Degut a la seva flexibilitat funcional i arquitectònica, l’ús de proteïnes recombinants formades per múltiples dominis representen una eina interesant pel desenvolupament de nanopartícules capaces de transportar fàrmacs de manera específica. Recentment, en el nostre grup, hem aplicat un nou sistema d’enginyeria pel desenvolupament de nanoparticles formades mitjançant l’oligomerització de proteïnes modulars. L’eficàcia selectiva i l’absència de toxicitat d’unes nanopartícules dirigides al càncer colorectal demostra la capacitat d’aquest enfoc biotecnològic. Un enfoc basat en l’ús de pèptids cat

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10

Comenale, Gabriela. "Expressão e purificação da proteína recombinante L2 do Papilomavírus bovino tipo-2 em sistema bacteriano." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-11102013-104819/.

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A papilomatose bovina é uma doença infectocontagiosa de ocorrência mundial, que assola o rebanho brasileiro, sem qualquer atitude efetiva de controle, e que tem como enfermidades associadas a tumores de bexiga hematúria enzoótica e tumores de trato digestório superior caraguatá, responsáveis por sensíveis perdas para a pecuária. Várias tentativas vacinais têm sido empreendidas com finalidades profiláticas ou terapêuticas, porém sem resultados eficazes. Esta situação se deve a aspectos relacionados à estrutura viral que dificultam uma manipulação eficiente para produção de produtos vacinais. Pa

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11

Carratalá, Tomás Jose Vicente. "Development and characterization of protein nanoformulations as alternative therapeutics to reduce antibiotic usage." Doctoral thesis, Universitat Autònoma de Barcelona, 2021. http://hdl.handle.net/10803/673321.

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L’aparició de farmacoresistència bacteriana als antibiòtics convencionals és una situació d’alarma mundial. Aquest escenari ha obligat a implementar mesures com la millora de les pràctiques d’higiene o l’administració controlada d’antibiòtics per reduir el seu ús en totes les àrees en les que s’utilitzen comunament, inclosa la medicina humana i animal i la indústria animal de producció d’aliments . Totes aquestes mesures estan destinades a disminuir l’aparició i propagació de la resistència als antibiòtics entre els bacteris, però quan es tracta de combatre als bacteris que ja presenten una o

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12

Nourizad, Nader. "Recombinant Enzymes in Pyrosequencing Technology." Doctoral thesis, KTH, Biotechnology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3765.

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<p>Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released.The released PPi is converted to ATP by ATP sulfurylase, whichprovides the energy to luciferase to oxidize luciferin andgenerate light. The excess of dNTP(s) and the ATP produced areremoved by the nucleotide degrading enzyme apyrase.</p><p>The commerci

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13

Delucchi, Anthony Benjamin. "Bacterial expression of radio-labeled recombinant proteins for studying AHR signalling." Scholarly Commons, 2001. https://scholarlycommons.pacific.edu/uop_etds/550.

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The ligand activated transcription factor Aryl Hydrocarbon Receptor (AHR) forms a DNA binding heterodimer with the Aryl Hydrocarbon Nuclear Translocator (ARNT) in response to planar aromatic hydrocarbons. In addition to AHR and ARNT there are at least three other proteins involved in AHR signaling. These proteins are the co-chaperone p23, Ara-9 and two molecules of Heat Shock Protein-90 (HSP-90). This study documents the production of Ara-9 and C∆418 (an ARNT deletion construct) in a modified thioredoxin fusion system. These proteins were expressed in a system that allowed for removal from the

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14

O'Hara, John F. "An investigation of post-translational processing in the transgenic mammary gland." Thesis, University of Kent, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365215.

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15

Teixeira, Lais Helena. "Geração e análise da imunogenicidade de proteínas recombinantes baseadas nas diferentes formas do antígeno circumsporozoíta de Plasmodium vivax visando o desenvolvimento de uma vacina universal contra malária." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/42/42133/tde-11072014-110149/.

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O P. vivax é a segunda espécie mais prevalente causadora de malária no mundo. Medidas de controle ineficientes exigem o desenvolvimento de novas estratégias de prevenção, como vacinas, novas drogas e novos inseticidas. O objetivo geral do trabalho foi gerar uma formulação vacinal universal com proteínas e adenovírus recombinantes capazes de induzir anticorpos contra as diferentes formas alélicas da proteína circumsporozoíta (CSP) do P. vivax. As proteínas foram produzidas em E. coli e purificadas por cromatografia de afinidade e troca iônica. A obtenção destas proteínas nos permitiu testar qua

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16

Adelantado, Vallvé Núria. "Lipidomics studies of recombinant Pichia pastoris for improved recombinant protein secretion through cell engineering." Doctoral thesis, Universitat Autònoma de Barcelona, 2016. http://hdl.handle.net/10803/384229.

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L’ús cada cop més extensiu de plataformes “òmiques” per a l’estudi de llevats ha generat grans quantitats d’informació relativa a la fisiologia cel·lular sota diferents condicions de cultiu, la qual pot ser utilitzada per al desenvolupament de noves estratègies d’enginyeria genètica. S’ha observat que els nivells d’expressió d’un fragment d’anticòs humà produït en el llevat metilotròfic Pichia pastoris augmenten significativament quan aquest és cultivat en nivells de baixa disponibilitat d’oxigen. Anàlisis transcriptòmics mostren que hi ha una regulació important dels gens involucrats en el me

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17

Thomas, Jeffrey G. "Molecular chaperones and the folding of recombinant proteins in Escherichia coli /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/9881.

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18

Gendel, Elizabeth Massey. "Improving recombinant membrane protein overexpression in E. coli." Diss., Restricted to subscribing institutions, 2009. http://proquest.umi.com/pqdweb?did=1997765431&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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19

Weeks, Stephen David. "Applications of fusion technologies in recombinant protein expression." Thesis, Lancaster University, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.428646.

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20

Abdullah, N. "Strategies for expanded bed purification of recombinant protein." Thesis, University of Cambridge, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.595323.

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True biospecific affinity chromatography, immobilized metal affinity chelating (IMAC) and ion exchange chromatography (IEX) strategies, were investigated for EBA purification of a model system, recombinant glutathione-S-transferase (GST). All three strategies were performed using an unclarified <i>E.coli</i> homogenate containing GST concentration at 20-25 % of the total protein, which represented a large initial degree of purity. True biospecific affinity strategy exploits the naturally occurring affinity between GST and its natural substrate, glutathione (GSH). EBA purification using STREAML

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21

Dwivedi, Gaurav Dutta. "Cloning and Expression of Streptococcal Recombinant Protein G." Thesis, Umeå universitet, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-106723.

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Recombinant Protein G (rPG), an engineered form of streptococcal protein G with a theoretical molecular weight of 22.26 kDa was successfully cloned and expressed in E.coli BL 21(DE3) cells. The albumin binding domain was removed during the gene synthesis to avoid unspecific binding. This recombinant form of protein G contains only the IgG binding domains along with the 6X histidine tag at the N terminal. The removal of non-specific domains maximizes the specificity of IgG binding through the Fc region. The recombinant protein G was purified through heat treatment and using immobilized metal af

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22

Stapelberg, Julanie. "Recombinant protein production potential of South African microalgae." Diss., University of Pretoria, 2019. http://hdl.handle.net/2263/71149.

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Therapeutic recombinant proteins such as antibodies, hormones, enzymes, and anticoagulants can be used in medicine, for food production, agriculture and bioengineering industries. Recombinant proteins are currently being produced by mammalian, plant, insect and microbial culture systems but microalgae, when used as an expression vector, would offer many benefits over existing methods. These advantages include, lower production costs, faster growth rates, easier culturing, simpler transgenic manipulation as well as modified abilities of transcription and translation. South Africa boasts one of

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23

Sun, Shijing. "Study of bacterial cellulose synthase by recombinant protein." 京都大学 (Kyoto University), 2017. http://hdl.handle.net/2433/225675.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第20450号<br>農博第2235号<br>新制||農||1050(附属図書館)<br>学位論文||H29||N5071(農学部図書室)<br>京都大学大学院農学研究科森林科学専攻<br>(主査)教授杉山淳司, 教授髙部圭司, 教授梅澤俊明<br>学位規則第4条第1項該当

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24

Barrero, Peña Juan José. "Overcoming the secretory limitations in Pichia pastoris for recombinant protein production." Doctoral thesis, TDX (Tesis Doctorals en Xarxa), 2020. http://hdl.handle.net/10803/670387.

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El llevat metilotròfic Pichia pastoris (genere Komagataella) s’ha convertit en una de les plataformes cel·lulars més populars per produir proteïnes d’interès industrial, la majoria de les quals es secreten extracel·lularment per facilitar el posterior procés de purificació. La secreció de proteïnes heteròlogues fora de la cèl·lula està mediada per la via secretora, una ruta que engloba diversos passos i té diferents orgànuls interconnectats per aconseguir una secreció eficient. Tanmateix, quan P. pastoris es veu obligat a produir proteïnes heteròlogues, el correcte funcionament de la via de se

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25

Tan, Yu Pei. "The development of Lactococcus lactis as an antimicrobial agent." Thesis, Queensland University of Technology, 2010. https://eprints.qut.edu.au/39143/1/Yu_Pei_Tan_Thesis.pdf.

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Non-pathogenic lactic acid bacteria are economically important Gram-positive bacteria used extensively in the food industry. Due to their “generally regarded as safe” status, certain species from the genera Lactobacillus and Lactococcus are also considered desirable as candidates for the production and secretion of recombinant proteins, particular those with therapeutic applications. The hypothesis examined by this thesis is that Lactococcus lactis can be modified to be an effective antimicrobial agent. Therefore, the aims of this thesis were to investigate the optimisation of the express

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26

Rossi, Merja. "Investigating cell type specific metabolism using GFP as a reporter protein." Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:0c418362-63e7-496d-9ff6-584a0c54c127.

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Metabolic flux analysis (MFA) is a powerful technique for quantifying the intracellular fluxes in central carbon metabolism. It relies on detection of stable isotope labelling from metabolites such as amino acids derived from protein. Current standard techniques are, however, unable to distinguish between different cell types in heterogeneous tissue. The aim of the thesis was to address this problem by developing and validating a strategy using green fluorescent protein (GFP) with cell type specific expression as a reporter protein for investigating the fluxes in specific cell types in the Ara

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Protopopova, Marina. "Modulation of activity of the tumour suppressor p53 by small molecules and damaged DNA /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-926-9/.

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28

Ridsdale, Ross Allan. "Studies of myelin basic protein, recombinant upstream binding factor and surfactant protein A." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ31864.pdf.

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29

Stenberg, Leisa M. "Expression and characterization of a recombinant human factor X/protein C chimeric protein." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape8/PQDD_0015/NQ46432.pdf.

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30

Bea, Joo-eun. "Generation of Baculovirus-Brucella Abortus Heat Shock Protein Recombinants; Mice Immune Responses Against the Recombinants, and B. Abortus Superoxide Dismutase and L7/L12 Recombinant Proteins." Diss., Virginia Tech, 1999. http://hdl.handle.net/10919/26244.

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<i>Brucella abortus</i> is capable of resisting the microbicidal mechanisms of phagocytic cells and growing within phagocytic cells, usually macrophages. <I>B. abortus</i>, like several other intracellular bacteria responds to the hostile environment in macrophages by producing heat shock proteins (HSPs) which are induced by environmental stresses. Bacterial HSPs are very immunogenic, eliciting both cellular and humoral immune responses in the infected host. The significance of host cellular and protective immune responses directed against these proteins is currently unresolved. Baculovirus re

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31

Garrigós, Martínez Javier. "Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris." Doctoral thesis, Universitat Autònoma de Barcelona, 2021. http://hdl.handle.net/10803/671119.

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La present tesi doctoral es centra en la caracterització de sistemes d’expressió utilitzats per a la producció de proteïnes recombinants (RPP) en el llevat metilotròfic Pichia pastoris. Al llarg de tot aquest treball s’integren els resultats de diferents camps -enginyeria de bioprocessos i regulació génica- per tal d’ampliar la informació sobre els sistemes d’expressió analitzats i, així, reduir la incertesa a l’dissenyar un bioprocés RPP. En el primer capítol de la tesi, s’estudia el sistema clàssic d’expressió basat en el PAOX1. En un primer pas, es van cultivar en quimiòstat dos clons pr

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32

Zhang, Zhigen. "Bioreactor studies of heterologous protein production by recombinant yeast." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq21404.pdf.

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33

Cornvik, Tobias. "Novel Technologies for Recombinant Protein Overexpression in Escherichia coli." Doctoral thesis, Stockholm : Dept. of Biochemistry and Biophysics, Stockholm university, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-861.

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34

Alfasi, Sara Nuri. "Physiological aspects underpinning recombinant protein production in Escherichia coli." Thesis, University of Birmingham, 2011. http://etheses.bham.ac.uk//id/eprint/1384/.

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Many biopharmaceutical projects require the production of recombinant protein in a bacterial host. Conventional procedures used for recombinant protein production (RPP) involve the rapid synthesis of the target protein. This results in the accumulation of unfolded protein, the induction of the heat shock stress response and bacterial growth arrest. More importantly, the target protein accumulates in inclusion bodies and hence is useless to determine its structure. The immediate impact of this is that both the yield and quality of the target protein are compromised. This thesis reports two gene

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Braun, Galleani S. C. "Exploring the potential for recombinant protein production in microalgae." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1458032/.

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Microalgae are considered as attractive platforms for the synthesis of high-value heterologous proteins due to their many beneficial attributes including ease of cultivation, lack of pathogenic agents, and low-cost downstream processing. However, recombinant protein levels are low compared to microbial platforms and commercial production is not a reality yet. Promising research using the model microalga Chlamydomonas reinhardtii has highlighted this potential, particularly for transgene expression in the chloroplast. The objective of this research was to study different strategies for expressi

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Holler, Christopher J. "Purification of an acidic recombinant protein from transgenic tobacco." Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/32379.

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Tobacco has been studied as a host for producing recombinant therapeutic proteins on a large-scale, commercial basis. However, the proteins expressed in tobacco usually need to be purified to high yield and purity from large amounts of biomass in order for their production to be commercially viable. The methods needed to purify proteins from tobacco are very challenging and not well studied. The objective of this research was to develop a process for the purification of the acidic model protein, recombinant Î²-glucuronidase (rGUS), from transgenic tobacco leaf tissue to high yield and purit

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Wangsa-Wirawan, Norbertus Djajasantosa. "Physicochemical properties of protein inclusion bodies." Title page, contents and introduction only, 1999. http://web4.library.adelaide.edu.au/theses/09PH/09phw2465.pdf.

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Bibliography: leaves 182-198. Improvements in the current production system of inclusion bodies and the downstream processing sequence are essential to maintain a competitive advantage in the market place. Optimisation of fermentation is considered to improve production yield; then flotation as a possible inclusion body recovery method.

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Buswell, Walter Scott. "Expression of recombinant porcine preprorelaxin in Nicotiana tabacum." Thesis, Virginia Tech, 2006. http://hdl.handle.net/10919/32803.

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Relaxin is a small peptide hormone that has demonstrated potential therapeutic actions for cardiovascular disease and fibrosis. Additionally, relaxin has demonstrated the ability to protect the heart from injuries caused by ischemia and reperfusion, promote the healing of ischemic ulcers, and counteract allergic responses. The objective of this research was to express fully processed porcine relaxin in transgenic tobacco plants, as an alternative to current methods of producing relaxin. <p> Two recombinant relaxin genes were constructed that contained the patatin signal peptide cDNA fused in

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Tomàs, Gamisans Màrius. "Developing strategies for systems metabolic engineering of Pichia pastoris." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/458538.

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Pichia pastoris s’ha convertit en una de les plataformes cel·lulars més utilitzades per a la producció de proteïnes recombinants i metabòlits d’alt valor afegit. En els darrers anys s’han aconseguit fites importants en l’anàlisi quantitativa a nivell de sistemes de la seva fisiologia. Aquesta gran quantitat d’informació ha permès desenvolupar models metabòlics a escala genòmica, que permeten el desenvolupament de noves estratègies per l’enginyeria de soques i de bioprocés. Amb anterioritat a aquest estudi s’havien publicat tres models metabòlics a escala genòmica per a P. pastoris. No obstant

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Teng, Weibing. "Characterization and Biomedical Applications of Recombinant Silk-Elastinlike Protein Polymers." Diss., The University of Arizona, 2012. http://hdl.handle.net/10150/242475.

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Biomaterials requirements nowadays are becoming more and more specialized to meet increasingly demanding needs for biomedical applications such as matrices for tissue scaffolds. Among various useful classes of biomaterials, protein-based materials have been extensively pursued as they can offer a wide range of material properties to accommodate a broader spectrum of functional and performance requirements. The advent of genetic engineering and recombinant DNA technology has enabled the production of new protein-based biopolymers with precisely controlled amino acid sequence. As an example, sil

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Lima, e. Castro Paula Maria. "Optimisation of CHO cell growth and recombinant interferon-γ production". Thesis, University College London (University of London), 1993. http://discovery.ucl.ac.uk/1317969/.

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The optimisation of recombinant protein production by animal cell cultures is important for the economic feasibility of these processes. Simultaneously with product yield, product authenticity is a crucial aspect to consider as it may per se affect the therapeutic value of such proteins. More defined culture media are being developed, particularly to ensure batch product consistency. A Chinese Hamster Ovary cell line (CHO 320) producing human interferon-γ (IFN-γ), a glycosylated protein, was chosen to investigate the effects of the culture environment on (I) cell growth, (2) product yield and

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Mejia, Lara Adrian Alberto. "Generation and isolation of recombinant DNase II enzyme." To access this resource online via ProQuest Dissertations and Theses @ UTEP, 2007. http://0-proquest.umi.com.lib.utep.edu/login?COPT=REJTPTU0YmImSU5UPTAmVkVSPTI=&clientId=2515.

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Robinson, Joanne Claire. "Structure and functional studies of the short consensus repeats of the human complement receptor type 1." Thesis, University of Southampton, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342847.

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Briggs, Geoffrey Shaw. "Folding and stability studies on papain and the effect of recombinant papain pro fragment." Thesis, University of Kent, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.281755.

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Díaz, Ocaña Raquel. "Recombinant self-assembling nanoparticles for cancer therapy based on toxin and venom compounds." Doctoral thesis, Universitat Autònoma de Barcelona, 2020. http://hdl.handle.net/10803/670483.

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La plataforma desenvolupada d’enginyeria de proteïnes auto-acoblables permet dissenyar nanopartícules únicament proteiques (NPs) capaces d’atacar i actuar selectivament sobre les cèl.lules canceroses mitjançant la interacció amb receptors que es sobreexpressen. Les estructures esfèriques estables de les NPs desenvolupades i la seva mida adequada, en combinació amb els pèptids d’orientació , milloren la seva especificitat. A més, la incorporació de segments de toxina i verí ha millorat els efectes terapèutics d’aquestes estructures que són totalment biocompatibles i que no tenen cap portador ex

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46

Hemminki, Ari. "Development of recombinant antibodies for diagnostic applications by protein engineering /." Espoo : Technical Research Centre of Finland, 1998. http://www.vtt.fi/inf/pdf/publications/1998/P365.pdf.

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Monforte, Mercado Sergi. "Systems metabolic engineering for recombinant protein production in Pichia pastoris." Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/669385.

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El llevat metilotròfic Pichia pastoris (Komagataella sp.) és un dels sistemes d’expressió més atractius per a la producció de proteïna recombinant, mercat contínuament en expansió. El fort promotor del gen de l’alcohol oxidasa 1 (PAOX1), induït per metanol però reprimit per glucosa, glicerol o etanol, és un dels més emprats per aquest propòsit. No obstant, existeixen encara diversos colls d’ampolla fisiològics que limitant el procés. En aquest context, diferents estratègies han estat proposades i provades per tal de millorar la producció heteròloga de molts tipus diferents de proteïna. Les ap

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McKenna, Tomás. "Oxidative stress on mammalian cell cultures during recombinant protein expression." Licentiate thesis, Linköping University, Linköping University, Biotechnology, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-51823.

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<p>When the cell is under stress arising from oxidation, heat, infection, toxic contamination or any other stressful condition, proteins may unfold and expose residues in their structure that under normal physiological conditions are hidden and shielded from chemical reactions.</p><p>In this licentiate thesis the effects of general oxidative stress on the production of recombinant protein by mammalian cells are considered.</p><p>The work consisted of a broad literary review focused on oxidative stress and cellular response, cross-protection, gene regulation in response to oxidative stress and

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Luther, Kelvin B. "Expression and purification of recombinant HIV-1 BH10 Tat protein." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/MQ62787.pdf.

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Ahuja, Sanjay. "Development of a recombinant protein vaccine against Plasmodium falciparum malaria /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-788-X/.

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